scholarly journals Characterization of the Chemical Components of the Extracted Carduus Pycnocephalus L, and Assessment of Its Potential Novel Antioxidant, Antibacterial, and Anticancer Activities

2021 ◽  
Author(s):  
Luay El Hayyany ◽  
El‐Sayed El‐Halawany ◽  
Hoda Soliman ◽  
Yasser El-Amier
Keyword(s):  
Medicinal Plant ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Tumor Cell Line ◽  
Antibacterial Activities ◽  
Extraction Process ◽  
Cytotoxic Agents ◽  
Chemical Components ◽  
Anticancer Activities ◽  
Biological Potency

Abstract Carduus pycnocephalus L, which is related to the Astraceae family, was well-known as a privileged medicinal plant that has innumerable respected biological potency. The current research aims to identify the chemical constitutes of the essential oils of the extracted C. pycnocephalus by Gas Chromatography-Mass Spectroscopic analysis (GC-MS) and to assess the biological profiles of the plant and its botanical ingredients as a precise antioxidant, and anticancer, as well as, antimicrobial agents. The extraction process of the medicinal plant by methanol provided a possibility to extract and identify the polar chemical constitutes that have the most effective categories of components. The DPPH antioxidant potency of the botanical ingredients of the plant indicated that the flower extract is the most potent with IC50 = 30.69 mg/L followed by leaves (IC50= 32.78 mg/L), stem (IC50= 41.31 mg/L), and root (IC50= 46.84 mg/L). The antibacterial activities of the root, stem, leaf, and flower extracts of C. pycnocephalus exhibited remarkable potency to kill or inhibit the growth of the bacterial species. Leaf, and flower extracts revealed the most potent activities than the antibiotic standards against E. coli, S. typhimurium, and B. cereus species with inhibition zones ranged from 20-26 mm. Also, the extracted C. pycnocephalus revealed a moderate cytotoxic effect against hepatocellular carcinoma (HepG2) tumor cell line using MTT assay with IC50= 46.2 µg/mL. The experimental interpretations inveterate the potential of C. pycnocephalus extract indicated its biological impacts as antioxidant, antibacterial, and moderate cytotoxic agents that provided the ease of using it in cancer therapy.

Phytochemical profiles and antimicrobial activities of garlic bulb extracts: Traditional medicinal plant in Maiduguri, Borno State

2021 ◽  
Vol 1 (1) ◽  
pp. 008-011
Author(s):  
Fatima Adamu Lawan ◽  
Ibrahim Yusuf Ngoshe ◽  
Binta Baba Shehu ◽  
Ali AG Benisheikh ◽  
Abdullahi Abdulkadir ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Bacterial Species ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Antibacterial Drug ◽  
Garlic Bulb ◽  
Phytochemical Profile ◽  
Phytochemical Profiles

The aim of present study was to assess phytochemical profile and antibacterial activities of some selected bacterial species. All the bacteria species tested showed higher inhibition zone varying from 20mm-27mm with 100mg/ml of ethyl acetate, methanol, Hexane and chloroform respectively. Moreover, the phytochemical profile revealed the presence of steroids, alkaloids, flavonoids and tannin in the extracts. Thus, result of this study showed that Garlic bulb extract could be a good candidate for production of synthetic antibacterial drug for the treatment of infections caused by the organisms used in this study.

scholarly journals In Vitro Antibacterial Activities of Various Ethanolic Medicinal Plant Extracts Against Some Human Pathogenic Bacteria

2020 ◽  
Vol 8 (6) ◽  
pp. 1272-1276
Author(s):  
Samir K. Ali ◽  
Ghorbat S. Ali ◽  
Berivan Abdulrahman Abdullah
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Nigella Sativa ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Foeniculum Vulgare ◽  
Medicinal Plant Extracts

The widespread use of antibiotics often causes increase in the bacterial drugs resistance and causes many side effects in humans. Medical plants have antimicrobial effects against most pathogenic bacteria and can serve as harmless replacement to antibiotics. The aim of this study was to assess the antibacterial effect of five medicinal plant prevailed in Kurdistan region namely; Cinnamon (Cinnamomum cassia (L.) D.Don), Nigella (Nigella sativa L.), Allium (Allium hirtifolium Boiss.), Carrot (Daucus carota L.), and Fennel (Foeniculum vulgare Mill.) against pathogenic Gram positive bacteria (Staphylococcus aureus), and pathogenic Gram negative bacteria (Salmonella typhi and Escherichia coli). The evaluation of antibacterial activity for these plant extracts was carried out using agar-well diffusion method. Results showed that minimum inhibitory concentration (MIC) of A. hirtifolium against tested bacterial isolates were (25 mg/mL), the lowest MIC values for S. aureus were (25 mg/mL) observed with C. cassia, N. sativa and F. vulgare ethanolic extracts and the lowest MIC of D. carota against bacterial isolates were (50 mg/mL), Also, it was observed that S. aureus was more sensitive than S. typhi and E. coli to plant extracts. The ethanol plant extracts had potential antibacterial activities. However, further studies are required to identify the active compounds which could be used for the preparation of new antimicrobial agents and control the bacterial infectious diseases.

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

2019 ◽  
Vol 12 (5) ◽  
pp. 4644-4647
Author(s):  
K.K. Gupta ◽  
Neha Kumari ◽  
Neha Sinha ◽  
Akruti Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Bacterial Species ◽  
Antimicrobial Property ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Light Yellow ◽  
Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

Antimicrobial properties of actively purified secondary metabolites isolated from different marine organisms

2020 ◽  
Vol 21 ◽  
Author(s):  
Nilushi Indika Bamunu Arachchige ◽  
Fazlurrahman Khan ◽  
Young-Mog Kim
Keyword(s):  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Cost Effective ◽  
Resistance Mechanisms ◽  
Marine Organisms ◽  
Antimicrobial Compounds

Background: The treatment of infection caused by pathogenic bacteria becomes one of the serious concerns globally. The failure in the treatment was found due to the exhibition of multiple resistance mechanisms against the antimicrobial agents. Emergence of resistant bacterial species has also been observed due to prolong treatment using conventional antibiotics. To combat these problems, several alternative strategies have been employed using biological and chemically synthesized compounds as antibacterial agents. Marine organisms considered as one of the potential sources for the isolation of bioactive compounds due to the easily available, cost-effective, and eco-friendly. Methods: The online search methodology was adapted for the collection of information related to the antimicrobial properties of marine-derived compounds. These compound has been isolated and purified by different purification techniques, and their structure also characterized. Furthermore, the antibacterial activities have been reported by using broth microdilution as well as disc diffusion assays. Results: The present review paper describes the antimicrobial effect of diverse secondary metabolites which are isolated and purified from the different marine organisms. The structural elucidation of each secondary metabolite has also been done in the present paper, which will help for the in silico designing of the novel and potent antimicrobial compounds. Conclusion: A thorough literature search has been made and summarizes the list of antimicrobial compounds that are isolated from both prokaryotic and eukaryotic marine organisms. The information obtained from the present paper will be helpful for the application of marine compounds as antimicrobial agents against different antibiotic-resistant human pathogenic bacteria.

Isolation and HPLC Determination of the Chemical Components of Gentianella acuta (Michx.) Hulten

2018 ◽  
Vol 15 (1) ◽  
pp. 21-33
Author(s):  
Ying Wei ◽  
Yongqiao Liu ◽  
Yifan Hele ◽  
Weiwei Sun ◽  
Yang Wang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
High Speed ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gradient Elution ◽  
Chemical Components ◽  
Isolation And Characterization ◽  
Major Chemical ◽  
Main Components ◽  
First Time

Background: Gentianella acuta (Michx.) Hulten is an important type of medicinal plant found in several Chinese provinces. It has been widely used in folk medicine to treat various illnesses. However, there is not enough detailed information about the chemical constituents of this plant or methods for their content determination. Objective: The focus of this work is the isolation and characterization of the major chemical constituents of Gentianella acuta, and developing an analytical method for their determination. Methods: The components of Gentianella acuta were isolated using (1) ethanol extraction and adsorption on macroporous resin. (2) and ethyl acetate extraction and high speed countercurrent chromatography. A HPLC-DAD method was developed using a C18 column and water-acetonitrile as the mobile phase. Based on compound polarities, both isocratic and gradient elution methods were developed. Results: A total of 29 compounds were isolated from this plant, of which 17 compounds were isolated from this genus for the first time. The main components in this plant were found to be xanthones. The HPLC-DAD method was developed and validated for their determination, and found to show good sensitivity and reliability. Conclusion: The results of this work add to the limited body of work available on this important medicinal plant. The findings will be useful for further investigation and development of Gentianella acuta for its valuable medicinal properties.

Effect of Silver Ions on the Structural and Antibacterial Properties of Bone Replacement Material

2013 ◽  
Vol 795 ◽  
pp. 692-696 ◽  
Author(s):  
Nida Iqbal ◽  
Mohammed Rafiq Abdul Kadir ◽  
Nasrul Humaimi Bin Mahmood ◽  
Micheal Moses ◽  
Mashitah Binti Mad Salim ◽  
...  
Keyword(s):  
Calcium Phosphates ◽  
Bacterial Species ◽  
Calcium Nitrate ◽  
Antibacterial Properties ◽  
Silver Ions ◽  
Antibacterial Activities ◽  
Silver Addition ◽  
Wide Range ◽  
Microbial Infections ◽  
Inorganic Mineral

Antibacterial materials based on calcium phosphates have wide range of biomedical applications in the prevention of microbial infections. The synthesis of inorganic mineral component of bone i.e. hydroxyapatite was done with the addition of silver (Ag) (5-15 wt %) as antibacterial agent. The wet precipitation synthesis was carried out using diammonium hydrogen phosphate and calcium nitrate as P and Ca precursors. The presence and effect of silver addition on the structure was studied using Fourier Transform-Infrared (FTIR) spectroscopy and Energy Dispersive X-ray (EDX) techniques. The antibacterial properties of all samples were evaluated using Disc Diffusion Technique (DDT) againstS. aureus,B. subtilis, P. aeruginosaandE. coli. Antibacterial activities of samples were found to vary depending on the bacterial species and Ag loading percentage. The antibacterial assay suggested that the addition of Ag ions within hydroxyapatite can be effectively provided the required level of antibacterial activity against bacteria.

Isolation and Identification of Multidrug-Resistant Isolates of Uropathogenic Klebsiella spp in Dogs

2021 ◽  
Vol 3 (1) ◽  
pp. 6-12
Author(s):  
M Mustapha ◽  
P Goel
Keyword(s):  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Multidrug Resistant ◽  
Urine Samples ◽  
Penicillin G ◽  
Isolation And Identification ◽  
Sensitivity Testing ◽  
Bacterial Organism ◽  
Tract Infections ◽  
Klebsiella Spp

The most widespread ailments in dogs are urinary tract infections (UTIs) caused by bacterial species. It is necessary to recognize the prevailing bacterial pathogens and their susceptibility to antimicrobial agents to effectively treat UTIs. The present study aimed to classify the bacterial organism that causes UTIs in dogs and their patterns of antimicrobial resistance. A total of 141 urine samples were collected from diseased dogs in Veterinary Clinical Complex LUVAS in Hisar, India. Culture, biochemical and sensitivity testing were performed for each of the urine samples based on standard method. Of the total 141 urine samples from dogs, 21 (14.9%) isolates were identified as Klebsiella spp. The isolates were found to be highly resistant to ampicillin (100%), penicillin G (100%), oxytetracycline (100%), enrofloxacin (85.7%), chloramphenicol (80.6%), ceftriaxone (76.2%) and cloxacillin (71.4%), while susceptibility was observed against gentamicin (100%), amikacin (100%) and neomycin (90.5%). In the current study, 19 out of 21 identified isolates were found to be multidrug-resistant. This study indicates that dogs in the study area are found to harbor highly resistant Klebsiella spp. Therefore, when deciding on the antibiotic regimen for UTIs cases, Vets should consider resistance profile of chosen antibacterial agents before usage in order to discourage dissemination of resistant organisms in the study area.

Antioxidant and anticancer activities of an Aporphine alkaloid isolated from Alphonsea sclerocarp

2018 ◽  
Vol 7 (1) ◽  
pp. 51-55
Author(s):  
Suman Joshi DSD ◽  
◽  
Chander Amgoth ◽  
Surya Narayana S ◽  
Padmavathi CH ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plant ◽  
Chronic Myeloid Leukaemia ◽  
Mtt Assay ◽  
Preparative Hplc ◽  
Spectroscopic Techniques ◽  
Anticancer Activities ◽  
Frap Assay ◽  
Aporphine Alkaloid ◽  
Dose Dependent

Herein, we report on the anticancer and antioxidant activity of an Aporphine alkaloid isolated from medicinal plant Alphonsea sclerocarpa. Conventional column chromatography and preparative HPLC methods were used to isolate and purify the alkaloid. Based on NMR, FTIR and MS/MS spectroscopic techniques the compound was identified as Crebanine. This is the first report on the presence of Crebanine from the leaves of Alphonsea sclerocarpa. Antioxidant activity was evaluated using DPPH and FRAP assay. The antioxidant activity was dose-dependent and activity increased with the increase in the concentration. The cytotoxicity assay was carried out using MTT assay. The results showed remarkable cytotoxic activity against K562 (CML-chronic myeloid Leukaemia blood cancer) cells with a % inhibition of 37.545 at 500µm concentration after an incubation of 24h

scholarly journals Screening Of Antibacterial Activity Of Nepeta Ciliaris Benth. Against Respiratory Tract Pathogens

1970 ◽  
Vol 8 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Gautam Shiv Shankar ◽  
M Navneet ◽  
Kumar Sanjay ◽  
M Prabhat
Keyword(s):  
Respiratory Tract ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Bacterial Species ◽  
Positive Control ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Aerial Parts ◽  
Antibacterial Potential

The aim of present study was to evaluate the antibacterial potential of various extracts (petroleum ether, acetone, methanol and aqueous) of Nepeta ciliaris against selected respiratory tract pathogens. The extracts from the aerial parts of N. ciliaris at concentration of 200 mg/ml were screened against three gram-positive (Staphylococcus aureus MTCC 1144, Streptococcus pneumoniae MTCC 655 and Streptococcus pyogenes MTCC 442) and one gram-negative (Pseudomonas aeruginosa MTCC 2474) bacterial pathogens. The agar well diffusion method was adopted to examine antibacterial and minimum inhibitory concentration (MIC) values of most effective extracts against the susceptible bacteria. Erythromycin was used as positive control to determine the sensitivity of the strains. Out of the four bacterial species tested, S. pneumoniae was the most susceptible. The acetone extract exhibited maximum activity against all the tested microorganisms while methanol extract showed activity against P. aeruginosa. The MIC values ranged from 40 to 50 mg/ml for all the organisms. The N. ciliaris is potentially a good source of antimicrobial agents. DOI: http://dx.doi.org/10.3126/kuset.v8i1.6049 KUSET 2012; 8(1): 100-103

scholarly journals An ethno medicinal plant: Antibacterial activities of Stachys lavandulifolia Vahl aqueous extract against common pathogens

2021 ◽  
Vol 3 (1) ◽  
pp. 0-0
Author(s):  
Hori Ghaneialvar ◽  
Naser Abbasi ◽  
Sania Saneei ◽  
akram Zangeneh ◽  
Mohammad Mahdi Zangeneh ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Antibacterial Activities
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