scholarly journals Effect of Vasodilator And Immunosuppressive Therapy On The Endothelial Dysfunction In Patients With Systemic Sclerosis

2021 ◽  
Author(s):  
Dipanjan Bhattacharjee ◽  
Sumantro Mondal ◽  
Ayindrila Saha ◽  
Sanchaita Misra ◽  
Sudipta Chatterjee ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Endothelial Dysfunction ◽  
Unmet Need ◽  
Serum Levels ◽  
Circulating Endothelial Cells ◽  
Gene Expressions ◽  
Group Iii ◽  
Group I ◽  
Treatment Naïve ◽  
Healthy Control

Abstract Background: A comparative analysis of flow-mediated-vasodilation (FMD), vasoactive angiogenic, and fibrogenic mediators between treatment-naive and treated Systemic sclerosis (SSc) patients is an unmet need.Aim and Objective: 1) To assess the FMD and different pathogenic mediators in SSc patients [treatment naïve (Group-I, n=24) on vasodilator (Group-II, n=10), on vasodilator + immunosuppressive (Group-III, n=22)], and in healthy control (HC), n= 20]. To assess the proportion of circulating Endothelial cells in Group-I.Materials and method: FMD was measured in all the participants. Serum levels of NO, ET1, NO/ET1, sVCAM, sICAM, TGFβ, IL-6, VEGF were measured along with the gene expressions of eNOS, iNOS, ET-1, TGFβ. CEC was measured in Group-I and HC. Results: FMD was significantly decreased in all SSc patients though receiving treatment.Upregulation of serum NO and ET concentrations were noted post-treatment with an unaltered NO/ET1 ratio. NO was positively correlated with FMD (r= 0.6), and negatively with TGFβ (r=-0.5). ET-1 showed less correlation with TGFβ (r=-0.5) but no significant correlation with FMD. Circulating endothelial cell (CEC) was significantly higher in Group-I (3.2%) than HC (0.8%) (p = 0.002), and it showed a good correlation with NO (r=-0.7, p=0.0001) and NO/ET1 (r=-0.6,p=0.007).Conclusion: Endothelial dysfunction was seen in SSc patients irrespective of treatment which might be due to the unaltered NO/ET1. NO might be the major driving molecule in the vascular pathophysiology of SSc.

scholarly journals The Use of Antifibrotic Recombinant nAG Protein in a Rat Liver Fibrosis Model

2019 ◽  
Vol 2019 ◽  
pp. 1-5
Author(s):  
Maha M. Arafah ◽  
Mohammad M. Al-Qattan ◽  
Durria A. Abdulmaged-Ahmed ◽  
Ghada A. Al-Nafesah ◽  
Nessrin Y. Jadu ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Rat Liver ◽  
Serum Levels ◽  
Senior Author ◽  
Control Group ◽  
Protein Markers ◽  
Group Iii ◽  
Group I ◽  
Cord Injury ◽  
Rat Liver Fibrosis

Objectives. The “nAG” protein is the key protein mediating the regeneration of amputated limbs in salamanders. The senior author (MMA) developed the original hypothesis that since “nAG” is a “regenerative” protein, it must be also an “antifibrotic’ protein. The antifibrotic properties were later confirmed in a rabbit skin hypertrophic scar model as well as in a rat spinal cord injury model. The aim of this study is to evaluate the potential therapeutic properties of the nAG protein in a rat liver fibrosis model. Methodology. Liver fibrosis was induced using intraperitoneal injections of carbon tetrachloride (CCL4). A total of 45 rats were divided equally into 3 groups: Group I (the control group) received normal saline injections for 8 weeks, Group II received CCL4 for 8 weeks, and Group III received CCL4 and nAG for 8 weeks. At the end of the experiment, the serum levels of 6 proteins (hyaluronic acid, PDGF-AB, TIMP-1, laminin, procollagen III N-terminal peptide, and collagen IV-alpha 1 chain) were measured. Liver biopsies were also taken and the stages of live fibrosis were assessed histologically. Results. The CCL4 treatment resulted in a significant increase in the serum levels of all 6 measured proteins. The nAG treatment significantly reduced these high levels. The degree of liver fibrosis was also significantly reduced in the CCL4/nAG group compared to the CCL4 group. Conclusions. nAG treatment was able to significantly reduce the serum levels of several protein markers of liver fibrosis and also significantly reduced the histological degree of liver fibrosis.

scholarly journals CD4 and CXCR5 in Patients with Diabetic Nephropathy

2021 ◽  
pp. 211-218
Author(s):  
Sara Zaghloul Abdelhameed ◽  
Wesam Salah Mohamed ◽  
Kamal Mohamed Okasha ◽  
Mohamed Kamal Zahra
Keyword(s):  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
Diabetic Patients ◽  
T Follicular Helper Cells ◽  
Group Iii ◽  
Tfh Cells ◽  
Helper Cells ◽  
Follicular Helper ◽  
Group I ◽  
Healthy Control

Background: Diabetes is a metabolic condition characterized by hyperglycemia caused by defects in insulin secretion, insulin activity, or both. Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus especially type 2 diabetes. Blood CXCR5+CD4+ T cells represent three subsets: T follicular helper 1 (Tfh1), Tfh2 and Tfh17 cells. Increasing the numbers of circulating Tfh cells and also stimulation of Tfh cells have been linked with autoimmune diseases and may have a role in pathogenesis of diabetic nephropathy. Aim of the Work: Was to investigate CD4 and CXCR5 in diabetic nephropathy patients and assess their role in the pathogenesis of diabetic nephropathy. Subject and Methods: This study was conducted on 20 diabetic patients without nephropathy (Group I), 20 diabetic nephropathy patients (Group II) and 20 apparently healthy control subjects (Group III). Complete history taking and full clinical examination were done. Assessments of CD4 and CXCR5 were done by flowcytometry (FACS caliber, BD, CA, USA). Results: The present study revealed a statistically significant difference between diabetic patients (Group I), diabetic nephropathy patients (Group II) and healthy control (Group III) regarding CD4+CXCR5+T follicular helper cells level. CD4+CXCR5+T follicular helper cells levels were significantly higher in diabetic nephropathy patients than in diabetic patients without nephropathy and control subjects. CD4+CXCR5+T follicular helper cells level positively correlated significantly with fasting blood glucose, postprandial blood glucose, hemoglobin A1c, creatinine, urea and urinary protein level, and negatively correlated with estimated GFR in diabetic nephropathy patients. Conclusions: This study showed that CD4+CXCR5+T follicular helper cells level might be a useful marker for prediction and diagnosis of diabetic nephropathy patients.

scholarly journals The usefulness of melatonin supplementation in postmenopausal women with Helicobacter pylori-associated dyspepsia

2020 ◽  
Author(s):  
Cezary Chojnacki ◽  
Marta Mędrek-Socha ◽  
Paulina Konrad ◽  
Jan Chojnacki ◽  
Aleksandra Błońska
Keyword(s):  
Serum Level ◽  
Postmenopausal Women ◽  
Epigastric Pain ◽  
Serum Levels ◽  
Duodenal Mucosa ◽  
Urinary Concentration ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
H Pylori

Abstract Background Dyspeptic syndrome in the form of epigastric pain are particularly frequent in postmenopausal women. The aim of the study was to assess the role of melatonin in chronic dyspepsia in this group of women, with consideration of Helicobacter infection.Methods The study comprised 152 subjects Including 30 healthy women (group I), 60 women with asymptomatic H.pylori infection (group II), and 64 women H. pylori infected with chronic dyspepsia (group III). Endoscopic examination, histological assessment of gastric end duodenal mucosa, urease breath test (UBT-13C), and serum levels of 17-β-estradiol, follicle stimulating hormone, melatonin and urinary concentration of 6-sulfatoxymelatonin were determined by immunoenzymatic method. In group III – 14-day antibacterial treatment was introduced with pantoprazole, amoxicillin and levofloxacin. Afterward, in 32 women was administered placebo(group IIIa), and in 32 women (group IIIb) melatonin at a dose 1 m/morning and 3 mg/at bedtime, for six months.Results No significant differences were assessed between serum level of female hormone. Serum level of melatonin in group I and in group II was similar – 12,5 ± 2,72pg/ml and 10,5±3,73 pg/ml (p>0,05), whereas in group III was lesser – 5,72±1,42 pg/ml (p<0,001). Eradication of H.pylori was obtained in 75,0% women in group IIIa, and in 84,3% in group IIIb (p>0,05). After 6 months dyspeptic symptoms resolved in 43,7% patients in group IIIa and in 84,3% in group IIIb (p<0,001). Conclusion Melatonin supplementation is useful to complex therapy of H. pylori-associated dyspepsia, particularly in postmenopausal women at whom secretion of this hormone is decreased.

Evaluation of prognostic factors in gene expression and clinicopathologic characteristics in patients with adjuvant chemotherapy for advanced gastric cancer.

2011 ◽  
Vol 29 (4_suppl) ◽  
pp. 61-61
Author(s):  
M. Azuma ◽  
K. Ishido ◽  
A. Takeuchi ◽  
A. Naruke ◽  
K. Higuchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Adjuvant Chemotherapy ◽  
Prognostic Factor ◽  
Intestinal Type ◽  
Diffuse Type ◽  
Gene Expressions ◽  
Group Iii ◽  
Type Group ◽  
Group I

61 Background: We reported TS expression is an independent prognostic factor in patients with gastric cancer who received postoperative adjuvant chemotherapy with S-1 at 2010 ASCO GI (abstract 32). These pharmacogenomic finding will help for choosing chemotherapeutic agents for personalized therapy in the future. Our aim was to indicate association of TS expression with clinicopathological characteristics in the same patient population. Methods: 39 patients with stage II or III advanced gastric cancer who underwent gastrectomy were analyzed. These patients received adjuvant chemotherapy with S-1 after surgery. Formalin-fixed, paraffin-embedded tumor tissues were dissected by the laser-captured microdissection technique and analyzed for target gene expressions using a quantitative real-time PCR. Results: There were no significant differences between stage II and III in TS gene expressions. TS expression (low ≤ 0.72, high > 0.72) and histological type (intestinal and diffuse) are evaluated for PFS and OS. Patients were classified as Group I (n = 5); low TS and intestinal type, Group II (n = 14); low TS and diffuse type, Group III (n = 13); high TS and diffuse type and Group IV (n = 7); high TS and intestinal type. There were significant differences between these four groups (Kaplan-Meier survival analysis, log-rank test, PFS: p = 0.0112 OS: p = 0.0128). The survival curve showed longer survival both PFS and OS in Groups 1 > 2 > 3 > 4. In low TS situation (responders as Group I and II), there is a trend in patients with intestinal type had a longer survival compared to diffuse type (Group I > II). In high TS situation (non-responders as Group III and IV), the result are opposite, there is a trend in patients with diffuse type had a longer survival compared to Intestinal type (Group III > IV). Conclusions: These data suggest that TS gene expression levels may be molecular markers of prognostic factor for patients with adjuvant chemotherapy for resectable gastric cancer. S-1 might be effect different behavior by both histological type and TS expression. Prospective studies are needed to validate these preliminary findings. No significant financial relationships to disclose.

scholarly journals Serum level of il-6 in patients of type-ii diabetes mellitus with and without retinopathy: a comparative study

2017 ◽  
Vol 16 (4) ◽  
pp. 525-529
Author(s):  
Nadeem Afzal ◽  
Rasheed Anjum ◽  
Ahmed Nadeem ◽  
Khursheed Javed ◽  
Faheem Shahzad ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Serum Level ◽  
Medical Science ◽  
Serum Levels ◽  
Cross Sectional ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Duration Of Disease ◽  
Group Ii

Aim: Pakistan has 6.9 million people with diabetes mellitus (DM) that will be doubled by 2025. A study was designed to determine serum levels of IL-6 in type 2 diabetes mellitus (T2DM) patients.Methods: It was a cross-sectional case-control study of 212 subjects. Group-I included 30 subjects without DM, Group-II had 30 T2DM without retinopathy and Group-III had 152 T2DM with retinopathy. IL-6 was determined by ELISA technique. Data was analysed using SPSS 17.0.Results: More females were in Group-II (83%) and Group-III (66%) compared to Group-I (30%). Higher age was in Group-II (49yrs) and Group-III (50yrs) compared to Group-I (34yrs). Mean duration of disease (in years) was more in Group-III (10.51) than Group-II (7.76). Highest mean level of IL-6 was in Group-II, followed by Group-I and Group-III. On comparison, gender, age, duration of disease and the level of IL-6, there was a significant difference while there was no significant difference between percentages of HbA1c. The logistic regression model suggested low levels of IL-6 in patients of diabetic retinopathy was an independent predictor of retinopathy in patients with T2DM.Conclusion: Serum level of IL-6 was low in patients of diabetic retinopathopathy as compared to patients with T2DM without retinopathy.Bangladesh Journal of Medical Science Vol.16(4) 2017 p.525-529

scholarly journals The Impacts ofChrysanthemum indicumExtract on Oxidative Stress and Inflammatory Responses in Adjuvant-Induced Arthritic Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Mei Dong ◽  
Dongsheng Yu ◽  
Naif Abdullah Al-Dhabi ◽  
Veeramuthu Duraipandiyan
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Responses ◽  
Onset Time ◽  
Basal Diet ◽  
Serum Levels ◽  
Diet Group ◽  
Group Iii ◽  
Group I ◽  
Chrysanthemum Indicum ◽  
Clinical Arthritis

Chrysanthemum indicumhas been used as a therapeutic agent against inflammation, hypertension, and respiratory conditions for many years. This research’s aim has been to examine the antioxidant impacts thatChrysanthemum indicumextract (CIE) has on the oxidative stress and inflammatory responses in adjuvant-induced arthritic (AA) rats. 40 rats were categorised into 4 groups according to a completely randomized approach: Group I involved normal control rats (CTRL) that received a basal diet; Group II involved arthritic control rats (CTRL-AA) that received the same diet; Group III involved rats that received a basal diet and 30 mg/kg CIE; and Group IV involved arthritic rats with the same diet as Group III rats (CIE-AA). After injection with complete Freund’s adjuvant, body weight, arthritis score, and the serum levels of TNF-α, IL-1β, IL-6, myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were assessed. The results demonstrated that CIE delayed the onset time of arthritis and decreased the clinical arthritis severity score (P<0.05). Observations of CIE-AA and CTRL-AA rats demonstrated that CIE alleviates oxidative stress and inflammatory responses in CIE-AA group. In conclusion, CIE alleviated oxidative stress and inflammatory responses, thereby highlighting its potential use as a candidate for clinical treatments of rheumatoid arthritis.

scholarly journals Protective effect of Phyllanthus fraternus against cyclophosphamide-induced nephrotoxicity in rats

2017 ◽  
Vol 6 (4) ◽  
pp. 984
Author(s):  
Rakesh Singh Moirangthem ◽  
Ngangom Gunindro ◽  
Dipdeba Singh Takhellambam ◽  
Sucheta Devi Khuraijam ◽  
N. Meena ◽  
...  
Keyword(s):  
Protective Effect ◽  
Aqueous Extract ◽  
Serum Levels ◽  
Albino Rats ◽  
Standard Diet ◽  
Solid Organ ◽  
Group Iii ◽  
Group I ◽  
Ad Libitum ◽  
Phyllanthus Fraternus

Background: Cyclophosphamide is commonly used against malignancies, such as leukemia and solid organ cancers. It can induce nephrotoxicity in cancer patients thereby complicating the initiation of chemotherapy. The study is to evaluate the effect of Phylllanthus fraternus against cyclophosphamide induced nephrotoxicity in albino rats.Methods: In this study, a total of 20 albino rats were divided into four groups of five each. Group I (normal control) received i.p. injection of normal saline. While, group II, III and IV received single dose (200 mg/kg b/w) of cyclophosphamide i.p. on day 1. Group III and IV received 200 mg/kg and 400 mg/kg of aqueous extract of Phyllanthus fraternus respectively p.o. daily for 10 days. All the groups were fed with standard diet and water ad libitum. Serum levels of creatinine, urea and albumin were estimated. Histopathology of renal tissues was compared among the groups.Results: The renal parameters significantly improved in groups III and IV after 10 days of treatment with the extract. The histopathology study also supported the finding.Conclusions: The aqueous extract of Phyllanthus fraternus possesses protective effect against cyclophosphamide induced nephrotoxicity.

scholarly journals The Effects of Asthma on the Stress Oxidative, Inflammation, and Endothelial Dysfunction Characteristics in Children with Severe Community-Acquired Pneumonia

2021 ◽  
Author(s):  
Ali Arjmand Shabestari ◽  
Pegah Mohaghegh ◽  
Habibeh kiyanrad ◽  
fatemeh imanparast
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Community Acquired Pneumonia ◽  
Healthy Children ◽  
Group Iii ◽  
Group I ◽  
Tnf Α ◽  
Group Ii ◽  
Stress Oxidative ◽  
Pai 1

Abstract Background: Pulmonary vascular endothelial activation, inflammation, and stress oxidative have been implicated in adverse clinical outcomes of community-acquired pneumonia (CAP). Although chronic lung problems such as asthma may affect the consequences of pneumonia, the exact mechanism of this effect remains unclear. The present study aimed to assess the effects of asthma on the oxidative stress, inflammation, and endothelial dysfunction biomarkers in children pneumonia.Methods: This cross-sectional study was performed at Amir Kabir Hospital affiliated to Arak University of Medical Sciences, Arak, Iran. Participants were 25 children with severe CAP and asthma (group I), 25 children with severe CAP (group II), and 25 healthy children (group III) with 2 to 6 years of age. Fasting blood samples were taken to the assay of serum malondialdehyde (MDA), total antioxidant capacity (TAC), tumor necrosis factor-alpha (TNF-α), soluble vascular cell adhesion molecule-1 (sVCAM-1), and Plasminogen activator inhibitor-1 (PAI-1).Results: We observed a significant reduction in TAC in groups I and II (0.997±0.22 and 1.23±0.21 mmol/l, respectively) compared with group III (1.46±0.19 mmol/l). This reduction was significantly higher in group I than in group II. Also, we observed a significant increase in MDA and TNF-α in groups I (2.57±0.40 µmol/l, 6.94±1.61 pg/ml, respectively) and II (6.94±1.61µmol/l, 5.54±1.84 pg/ml, respectively) compared with group III (1.89 ±0.27µmol/l, 3.42±1.32 pg/ml, respectively). The increase in MDA was significantly higher in group I than in group II. VCAM-1 and PAI-1 as endothelial dysfunction biomarkers increased significantly in group I (1.5 ±0.62 mmol/l and 10.52±3.2 AU/ml, respectively) compared with groups II (1.06±0.53 mmol/l and 8.23±3.4 AU/ml, respectively) and III (0.6± 0.35 mmol/l and 2.39 ± 0.83 AU/ml, respectively). Also, VCAM-1 and PAI-1 increased significantly in group II compared with groups III.Conclusions: Asthma can exacerbate the consequences of pneumonia in children by increasing oxidative stress, inflammation, and endothelial dysfunction.

scholarly journals Hepatoprotective potential of Trigonella foenum graecum in deltamethrin induced albino rats

2014 ◽  
Vol 2 (04) ◽  
pp. 01-08
Author(s):  
Uma Nath Tripathi ◽  
Deepak Chandra
Keyword(s):  
Serum Levels ◽  
Hepatic Tissue ◽  
Albino Rats ◽  
Transferase Activity ◽  
Glutathione S Transferase ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Trigonella Foenum Graecum ◽  
Group I

Objective: Aim of investigation focuses attention on hepatoprotective and antioxidative effect of aqueous extract of Trigonella foenum graecum (TFG) in hepatic tissue of deltamethrin fed rats. Methods: In a 45 days treatment, rats were divided into six groups (IVI) of six animals in each, experiments were repeated thrice. Group I served as control rats; Group II received TFG dose 1 (9 g seed powder/kg b. wt./day); Group III received TFG dose 2 (45 g seed powder/kg b. wt./day); Group IV received deltamethrin; Group V received both deltamethrin and TFG (9 g seed powder/kg b. wt./day) and Group VI received both deltamethrin and TFG (9 g seed powder/kg b. wt./day). Results: In the present study, higher dose of TGF did not affect the levels of hepatic marker enzymes, which suggests that this dose had no toxic effect on normal rats. Significant increases in the serum levels of hepatic markers enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AP) were observed in deltamethrin treated rats. Furthermore, antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) activity and reduced glutathione (GSH) content were decreased in hepatic tissue of deltamethrin treated rats. Additionally, serum cholesterol and hepatic lipid peroxidation were significantly enhanced. Co-administration of TFG and vitamin C to the group V and VI restored all the parameters cited above to near-normal values. Conclusion: The result obtained from present study revealed that TFG appeared to be a promising agent for protection against deltamethrin induced hepatotoxicity.

scholarly journals The Effects of Asthma on the Stress Oxidative, Inflammation, and Endothelial Dysfunction Characteristics in Children with Severe Community-Acquired Pneumonia

2021 ◽  
Author(s):  
ali Arjmand Shabestari ◽  
fatemeh imanparast ◽  
pegah mohaghegh ◽  
habibeh kiyanrad
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Community Acquired Pneumonia ◽  
Healthy Children ◽  
Group Iii ◽  
Group I ◽  
Tnf Α ◽  
Group Ii ◽  
Stress Oxidative ◽  
Pai 1

Background: Pulmonary vascular endothelial activation, inflammation, and stress oxidative have been implicated in adverse clinical outcomes of community-acquired pneumonia (CAP). Chronic lung problems such as asthma may affect the consequences of pneumonia.The present study aimed to assess the effects of asthma on the oxidative stress, inflammation, and endothelial dysfunction biomarkers in children pneumonia. Methods: This cross-sectional study was performed at Amir Kabir Hospital affiliated to Arak University of Medical Sciences, Arak, Iran. Participants were 25 children with severe CAP and asthma (group I), 25 children with severe CAP (group II), and 25 healthy children (group III) with 2 to 6 years of age. Fasting blood samples were taken to the assay of serum malondialdehyde (MDA), total antioxidant capacity (TAC), tumor necrosis factor-alpha (TNF-α), soluble vascular cell adhesion molecule-1 (sVCAM-1), and Plasminogen activator inhibitor-1 (PAI-1). Results: We observed a significant reduction in TAC in groups I and II compared with group III. This reduction was significantly higher in group I than in group II. Also, we observed a significant increase in MDA and TNF-α in groups I and II compared with group III. The increase in MDA was significantly higher in group I than in group II. VCAM-1 and PAI-1 as endothelial dysfunction biomarkers increased significantly in group I compared with groups II and III. Also, VCAM-1 and PAI-1 increased significantly in group II compared with groups III. Conclusions: Asthma can exacerbate the consequences of pneumonia in children by increasing oxidative stress, inflammation, and endothelial dysfunction.

Sign in / Sign up

Export Citation Format

Share Document