The Integrated Analysis of Differential Long Non-Coding RNAs Expression Profiles in Extracellular Vesicles Derived From Chemoradioresistant and Parental Nasopharyngeal Carcinoma Cells
Abstract The patients with nasopharyngeal carcinoma (NPC) suffer from poor outcomes after chemoradiotherapy. Extracellular vesicles (EVs) play crucial roles in regulating cancer progression and chemoradioresistance. To investigate the functional role of chemoradioresistant cell-derived EVs for parental cells, and long non-coding RNAs (lncRNAs) expression profiles in EVs secreted by chemoradioresistant cells. The effect of chemoradioresistant NPC cell-derived EVs on migration and invasion abilities of parental cells were detected by Transwell assays. Human cancer lncRNA PCR array was performed on EVs released from chemoradioresistant NPC cells (CNE1R and CNE2R) and parental cells (CNE1 and CNE2). The concurrent chemoradioresistance of CNE1R and CNE2R was significant higher than that of CNE1 and CNE2. Transmission Electron Microscopy (TEM), Nanoparticle Tracking Analysis (NTA), and western blotting results showed the CNE1, CNE2, CNE1R and CNE2R -derived EVs were successfully obtained. The chemoradioresistant cell-derived EVs remarkably promoted migration and invasion ability of parental cells with different differentiation. Notably, a total of 91 differentially expressed (DE) lncRNAs were regulated in EVs by chemoradiotherapy. Among them, the 4 upregulated (MALAT1, FAM212B-AS1, LINC-PINT and H19) and 7 down-regulated (SNHG16, CDKN2B-AS1, ZFAS1, CCAT1, SNHG6, GAPLINC and TUG1) DE lncRNAs associated with chemoradioresistance, were identified in EVs derived from chemoradioresistant cells with different differentiation. We found the EVs derived from chemoradioresistant NPC cells regulated an aggressive phenotype of NPC cells, and identified an altered lncRNAs expression pattern in chemoradioresistant NPC cell- derived EVs.