scholarly journals Influence of different concentrations of Sildenafil Citrate on histological parameters of mice epididymis

2011 ◽  
Vol 5 (2) ◽  
pp. 55-60
Author(s):  
Usama I. M. Al-Zubaidi ◽  
Muhammad-Baqir M-R. Fakhrildin
Keyword(s):  
Sildenafil Citrate ◽  
Laboratory Animals ◽  
Control Group ◽  
Epithelial Layer ◽  
Male Mice ◽  
Sexual Performance ◽  
Histological Changes ◽  
Treatment Groups ◽  
And Control ◽  
Different Doses

sildenafil citrate (SC) is widely used for the treatment of erectile dysfunction and maintains a sufficient erection for satisfactory sexual performance. Very limited literatures were published about effects of SC on histological changes of epididymes in human or laboratory animals. The aim of this study was to investigate the influence of different concentrations of SC for different administration periods on some histological parameters of mice epididymes. Seventy five male mice (7-8) weeks old, weighing (23-25)g were used in the present study. The possible effect of SC was tested through the treatment groups of mice with different doses of SC (2, 4, 8, 16)mg/Kg body weight, and for different periods (1, 3, 5) weeks. Histological examination of epididymes were carried out and compared with those belong to untreated group. Results revealed no significant differences (P>0.05) in epididymal weight, tubules diameters and thickness of epithelial layer between treated groups and control group. Conclusion in this study, SC has no effect on epididymis histology

The Effects of Prednisolone and Serum Malondialdehyde Levels in Puppies with Experimentally Induced Meconium Aspiration Syndrome

2003 ◽  
Vol 31 (2) ◽  
pp. 113-122 ◽  
Author(s):  
E Kirimi ◽  
O Tuncer ◽  
M Kösem ◽  
E Ceylan ◽  
A Tas ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Standard Dose ◽  
Meconium Aspiration Syndrome ◽  
The Other ◽  
Control Group ◽  
Histological Changes ◽  
Meconium Aspiration ◽  
Treatment Groups ◽  
Different Doses ◽  
Experimentally Induced

The aim of this study was to investigate the effect of different doses of prednisolone in puppies experimentally induced with meconium aspiration syndrome (MAS). Meconium was collected from human babies in the first day of life and was released into the trachea of 11 newborn puppies to induce MAS. Puppies were treated with 2 mg/kg prednisolone (standard dose), 30 mg/kg prednisolone (megadose) or 0.9% saline, all administered intravenously. The study ended 20 h after meconium aspiration and the lungs were then scored for histopathology. Animals not treated with prednisolone deteriorated after 8 h while respiration rate, oxygenation, pH and partial pressure of carbon dioxide values were better in the prednisolone-treated groups. Histopathology scores were better in the treatment groups compared with the control group, with megadose giving the best result. At the end of the study, serum malondialdehyde levels were significantly higher in the megadose prednisolone group compared with the other two groups. In conclusion, we determined that prednisolone reduced physiological and histological changes in puppies with MAS and that a 30 mg/kg dose was more effective than 2 mg/kg.

Efficacy of Ovsynch and Ovsynch Plus Protocol for Improvement of Fertility in Postpartum Sahiwal Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
J. P. Lakher ◽  
M. K. Awasthi ◽  
J. R. Khan ◽  
M. R. Poyam
Keyword(s):  
Fixed Time ◽  
Conception Rate ◽  
Control Group ◽  
Group Iii ◽  
Treatment Groups ◽  
Group I ◽  
Early Postpartum ◽  
The Status ◽  
Sahiwal Cows ◽  
And Control

The present study was conducted to investigate the efficacy of Ovsynch and Ovsynch plus protocol in postpartum (day 60) Sahiwal cows (n=18). Animals were randomly divided into three equal groups, viz., Ovsynch group Ovsynch plus group and Control group. Animals of group I (n = 6) were treated with traditional Ovsynch protocol. The animals (n = 6) of group II were treated with Ovsynch plus protocol which consisted of an initial intramuscular injection of eCG (Folligon) @ 250 IU on day 60 postpartum followed 3 days later by GPG (Ovsynch) protocol. In group-III Control, no treatment was given to animals (n = 6). Treated animals were inseminated at a fixed time between 14 and 20 hrs after second GnRH injection, irrespective of estrus detection. Blood samples were collected from each animal on days 50 and 60 postpartum to determine the status of cyclicity in animals based on serum concentrations of progesterone (P4). A third blood sample was collected on the day of prostaglandin treatment to determine the response of first GnRH injection. Four animals each were cyclic, and two were acyclic in both treatment groups. Four animals each responded to first GnRH treatment in both treatment groups. Similarly, two animals each got conceived giving conception rate of 50% (2/4) in each treatment. In the control group, one out of 6 animals got conceived yielding 16.66 % conception rate (1/6) during the study period. It may be thus concluded that Ovsynch and Ovsynch plus protocol may be used during the early postpartum period to improve the reproductive efficiency in postpartum Sahiwal cows.

scholarly journals Safety Study on Ketoprofen in Pigs: Evaluating the Effects of Different Dosing and Treatment Scheme on Hematological, Hepatic, and Renal Parameters

2021 ◽  
Vol 8 (2) ◽  
pp. 30
Author(s):  
Luis Emilio Fazzio ◽  
Santiago José Raggio ◽  
Juan Facundo Romero ◽  
Juver Membrebe ◽  
Antonio Humberto Hamad Minervino
Keyword(s):  
Body Condition Score ◽  
Clinical Signs ◽  
Study Groups ◽  
Control Group ◽  
Safety Study ◽  
Treatment Groups ◽  
Treatment Scheme ◽  
Condition Score ◽  
Biochemical Evaluation ◽  
Different Doses

A safety study on ketoprofen 10% was carried out on pigs using a different dosing and treatment scheme. Forty healthy crossbreed pigs with similar age, weight, and body condition score were distributed into five treatment groups. The pigs were intramuscularly injected once with different doses of ketoprofen: 3 mg/kg (group 1X), 6 mg/kg (group 2X), 9 mg/kg (group 3X). In addition, the 3 mg/kg dosis was administered on three consecutive days (group 1X ext.). Intramuscular injections of saline solution were used in control group (CTL). The pigs were clinically examined throughout the trial and blood samples were taken for hematological and biochemical evaluation on days −4 (before treatment), +3, +7, and +14 (the end of the trial). Any unusual behaviour or clinical signs were reported as potential toxic effects of ketoprofen. Serum measurements showed that none of the ketoprofen doses produced changes in renal or hepatic biochemical parameters, liver enzymes, or total bilirubin. Likewise, hematological assessment indicated no altered parameters or hematocrit percentage in the study groups. These results demonstrate that ketoprofen has no adverse effects in pigs when the doses and scheme evaluated in this study are applied.

Significance of graded doses of aqueous seed extract of Moringa oleifera (L) on live body weight, gonadal, extragonadal dimensions and sperm reserves of Yankasa rams

2021 ◽  
pp. 33-40
Keyword(s):  
Body Weight ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
The Body ◽  
Control Group ◽  
Live Body Weight ◽  
And Control ◽  
Standard Techniques ◽  
Different Doses ◽  
Apparently Healthy

The aim of the study was to investigate the effects of Moringa oleifera aqueous seed extract on live body weight, gonadal and extragonadal dimensions and sperm reserves of Yankasa rams. Twenty five apparently healthy Yankasa rams aged 1-2 years and weighing 19.0 ± 2.1 Kg were used for the study. The rams were randomly selected into five groups: A, B, C, D and E with five rams in each group as treatment and control groups respectively. Groups A - D were given oral dose of Moringa oleifera aqueous seed extract at a dose rate of 1000, 2000, 3000, 4000 (mg/kg), respectively while group E was given 10 ml/kg water orally, daily for five months. Live body weight, gonadal and extragonadal reserves were determined according to standard techniques. The results showed a significant increase in live body weight in the months of April to June among rams treated with different doses of Moringa oleifera aqueous seed extract compared with the control group. The control group showed no significant differences in the body weight, gonadal and extragonadal dimensions and sperm reserves. In conclusion, the treatment of Yankasa rams with Moringa oleifera aqueous seed extract increased live body weight, but had no significant effects on gonadal and extragonadal dimensions and sperm reserves in Yankasa rams. Therefore, it is recommended that M. oleifera aqueous seed extract can be used at doses of 2000mg/kg to 3000mg/kg in Yankasa rams for optimum gain in live body weight.

scholarly journals 136 EVIDENCE OF A DIRECT EFFECT OF P4 ON IVF-DERIVED BOVINE 8-CELL EMBRYOS

2005 ◽  
Vol 17 (2) ◽  
pp. 219 ◽  
Author(s):  
C.E. Ferguson ◽  
T.R. Davidson ◽  
M.R.B. Mello ◽  
A.S. Lima ◽  
D.J. Kesler ◽  
...  
Keyword(s):  
Embryo Development ◽  
Direct Effect ◽  
Blastocyst Stage ◽  
Control Group ◽  
Bovine Embryo ◽  
Bovine Embryos ◽  
Direct Role ◽  
Treatment Groups ◽  
And Control

There has been much debate over a direct role for progesterone (P4) in early bovine embryo development. While previous attempts to supplement bovine embryos in vitro with P4 produced results that vary and are often contradictory, this may be a response of administering P4 at inappropriate times. Therefore, the objective of these experiments was to determine if P4 could exert a direct effect on developing IVF-derived bovine embryos when administered at an appropriate time of embryo development. In Exp. I, IVF-derived bovine 8-cell embryos were randomly allotted to treatments: (1) control, CR1aa medium (n = 168); (2) vehicle, CR1aa + ETOH (0.01%) (n = 170); and (3) P4, CR1aa + ETOH + P4 (20 ng/mL in 50-μL droplet) (n = 173). In Exp. II, IVF-derived bovine 8-cell embryos were randomly allotted to treatments: (1) control, CR1aa medium (n = 160); (2) vehicle, CR1aa + DMSO (0.01%) (n = 180); and (3) P4, CR1aa + DMSO (0.01%) + P4 (20 ng/mL in 50-μL droplet) (n = 170). All embryos were evaluated on Days 6 to 9 post-insemination and rates calculated from 8-cell embryos. In Exp. I, ETOH tended to have a detrimental effect with significantly fewer (P < 0.05) embryos (53%) developing to the blastocyst stage on Day 7 compared with the control (62%) and P4 (71%) groups. At Day 7, significantly more embryos cultured in P4 (71%) developed to the blastocyst stage compared with the control group (62%). P4 treatment significantly increased the number of Grade 1 blastocysts (25%) on Day 7 compared with vehicle (15%) and control (17%) groups. At the end of culture, there were also significantly more Day 9 hatched blastocysts in the P4 group (33%) compared with vehicle (22%) and control (21%) groups. Supplementing P4 in the culture medium increased the rate of development, resulting in significantly more blastocysts (8%) on Day 6 and hatched blastocysts (21%) on Day 8 compared with vehicle (3% and 12%) and control (0% and 8%) groups, respectively. In Exp. II, there were no significant differences between treatment groups for Day 7 blastocysts (control 54%, DMSO 61%, P4 57%) and Day 9 hatched blastocysts (control 46%, DMSO 51%, P4 46%). However, there were significantly more Grade 1 blastocysts in the P4 group (22% and 36%) on Days 6 and 8 compared with vehicle (11% and 23%) and control (13% and 23%) groups, respectively. The lack of improvement in Day 7 blastocysts and Day 9 hatched blastocysts rates leads to further uncertainty in understanding the P4 vehicle interactions. In conclusion, the results of these two experiments indicate that P4 can exert a direct effect on the developing IVF-derived bovine embryo; however, due to P4 vehicle interactions; other inert vehicles need to be explored to further evaluate the direct effects of P4 on the developing bovine embryo.

scholarly journals Pengaruh Terapi AIUEO terhadap Kemampuan Bicara Pasien Stroke yang Mengalami Afasia Motorik

2019 ◽  
Vol 1 (2) ◽  
pp. 226-235
Author(s):  
Afnijar Wahyu ◽  
Liza Wati ◽  
Murad Fajri
Keyword(s):  
Wilcoxon Test ◽  
Control Group ◽  
Stroke Patients ◽  
Control Groups ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Motor Aphasia ◽  
A Value ◽  
And Control

The purpose of this study was to determine the effect of AIUEO therapy on the speech ability of stroke patients who have motor aphasia in Raja Ahmad Thabib Hospital Tanjungpinang. The research design used was quasi experiment with the Nonequivalent Control Group Design approach to 9 respondents who were divided into 9 treatment groups and 9 control groups. The results showed that there were significant differences in the functional ability of communication between the control and treatment groups with a value of p <0.05 (p = 0.007 at a = 0.05) using the Wilcoxon Test statistical test. Conclusion, the influence of AIUEO therapy on the speech ability of stroke patients with motor aphasia in the treatment and control groups at Ahmad Thabib Hospital Tanjungpinang.   Keywords: Speech Ability, Motor Aphasia Stroke, AIUEO Therapy

scholarly journals Penggunaan Musik Daerah Untuk Meningkatkan Kompetensi Kecepatan Dan Ketepatan Mengetik Mahasiswa

2017 ◽  
Vol 2 (3) ◽  
pp. 12
Author(s):  
Maya Setiawardani ◽  
Tintin Suhaeni
Keyword(s):  
Instrumental Music ◽  
Traditional Music ◽  
Individual Performance ◽  
Control Group ◽  
Treatment Groups ◽  
Average Value ◽  
Typing Skills ◽  
Regional Music ◽  
Almost All ◽  
And Control

Mastery of keyboarding being part urgent to communicate through the computer, and very important, especially students who study in the field of business. On the other side, individual performance also influenced by the work environment, and music is one form the environment that could effect keyboarding performance. A model that can describe this is the stimulus-responsible model of mehrabian-russell which explains that music can affect the performance of individual writing. Almost all of Indonesia has traditional music. Therefore, research is needed to identify the effects of traditional music to enrich the area of innovation in strategies and techniques of learning keyboarding skills. In this study conducted an experiment on two groups, the treatment groups(traditional music) and control group (without traditional) and questionnaire to strengthen the experimental results. In preliminary tests of typing, both groups had a mean AWPM identical or similar. Furthermore, tests carried one, where the treatment group listened to five kinds of music Java, Sunda, and Bali. The test result and the result of the questionnaire showed that (typing skills shown by the average value of AWPM) will increase significantly used of traditional music as the background. So, the genre of traditional music can be stimulant to improve average performance of AWPM. There are three traditional kinds of music that can improve student typing speed and accuracy significantly, a regional music cublek-cublek suweng, kebiar bali, and oleg tambuliningan, but cublek-cublek suweng is a genre of traditional music that has the best effect. Therefore, the process of learning courses keyboarding skills needs to use instrumental traditional music, especially instrumental music that has a fast and cheerfully tempo.

scholarly journals The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Salman MO ◽  
Al-Wasiti EA ◽  
Thamir KA ◽  
Al-Ani IM ◽  
Al-Salihi AR
Keyword(s):  
Single Cell ◽  
Dna Fragmentation ◽  
Gel Electrophoresis ◽  
Testicular Tissue ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Sham Operation ◽  
Male Mice ◽  
Histological Changes ◽  
Single Cell Gel Electrophoresis

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

scholarly journals The safety of tea agarwood (Aquilaria malaccensis) from tree induction through test of toxicity subchronic oral 90 days

2017 ◽  
Vol 14 (2) ◽  
pp. 69-76
Author(s):  
RIDWANTI BATUBARA ◽  
SURJANTO SURJANTO ◽  
TAHAN MANGARANAP SIHOMBING ◽  
HERAWATY GINTING
Keyword(s):  
Clinical Symptoms ◽  
Laboratory Animals ◽  
Control Group ◽  
Male Mice ◽  
Tea Leaves ◽  
Subchronic Toxicity ◽  
Female Mice ◽  
Aquilaria Malaccensis ◽  
Repeated Doses ◽  
Histopathological Results

Batubara R, Surjanto, Sihombing TM, Ginting H. 2016. The safety of tea agarwood (Aquilaria malaccensis) from tree induction throuht test of toxicity subcronic oral 90 days. Biofarmasi 14: 69-76. Subchronic toxicity test is a test to detect the toxic effect that arises after the administration of the test reparation with repeated doses were given orally to the tested animal for 28 or 90 days. Leaves agarwood (Aquilaria malaccensis Lamk) is a tree from a tribe Thymeleaceae, already started popular used the farmer agarwood in Langkat as a drink that in pour. The result of an interview with the farmer agarwood explained that consume tea from the leaves agarwood of this kind of have many benefits include improve canal. To that was done the research security against the tea leaves agarwood induction taken from agriculture agarwood in Langkat, Sumatera North through test toxic subchronic oral. This study aims to determine the symptoms of toxic posed by product tea agarwood induction. This study used laboratory animals that male mice and female mice were divided into 5 groups, namely the 130, 260, 390, 520 mg/kgBW and the control group. The observation of clinical symptoms indicate the presence of toxic symptoms of weakness, changes in fur and agitated at doses of 390 and 520 mg/kgBW in male mice and female mice, the observation macropathology organs alloxan still normal the red-brown, the surface of slippery and consistency chewy. Histopathological results showed hemoglia and dilation of the blood vessels in all groups. Results showed that mice were given tea steeping agarwood induction doses ranging from 130, 260,390 and 520 mg/kgBW there are no mice died, so it can be concluded that the administration of agarwood tea steeping in mice does not cause toxic symptoms and safe for consumption.

scholarly journals NI-11 PATHOLOGICAL ASSESSMENT IN THE IMPACT OF ONE-SHOT BEVACIZUMAB ON UPTAKE OF 11C-METHIONINE

2019 ◽  
Vol 1 (Supplement_2) ◽  
pp. ii27-ii28
Author(s):  
Takaaki Beppu ◽  
Yuichi Sato ◽  
Kuniaki Ogasawara
Keyword(s):  
Amino Acid ◽  
Microvascular Density ◽  
Control Group ◽  
Tumor Removal ◽  
Histological Changes ◽  
Positron Emission ◽  
Newly Diagnosed Glioblastoma ◽  
And Control ◽  
The Impact ◽  
Single Intravenous Administration

Abstract BACKGROUND This study aimed to clarify how one-time administration of bevacizumab (BEV) changes histological features in glioblastoma, and how histological changes affect the uptake of 11C-methyl-L-methionine (11C-met) as an amino-acid tracer. MATERIALS AND METHODS Subjects were 18 patients with newly diagnosed glioblastoma who were assigned to 2 groups: BEV group, single intravenous administration of BEV before surgical tumor removal; and control group, surgical tumor removal alone. After surgery, we compared the densities of tumor cells and microvessels, and microvascular structures including vascular pericytes and L-type amino acid transporter-1 (LAT1), between the BEV and control groups. Correlations between 11C-met uptake on positron emission tomography before surgery, microvascular density, and LAT1 expression were assessed in each group. RESULTS BEV induced significant reductions in microvascular density, while tumor cell density and proliferation were retained in the BEV group. Percentages of vessels with pericytes and vascular endothelium with LAT1 expression were lower in the BEV group than in controls. Uptake of 11C-met correlated significantly with microvascular density in the BEV group, but not with LAT1expression. CONCLUSIONS The present study showed that even one course of BEV administration induced reductions in microvessels, vascular pericytes, and LAT1 expression in glioblastomas. One course of BEV therapy also reduced 11C-met uptake, which might have been largely attributed to reductions in microvessels rather than reductions in LAT1 expression, in addition to reduction of vascular permeability.

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