scholarly journals UJI SINERGIS KONSORSIA BAKTERI INDIGEN LCN DENGAN KONSORSIA BAKTERI TANAH DI KEBUN PERCOBAAN UNIVERSITAS MUHAMMADIYAH METRO SEBAGAI SUMBER BELAJAR BIOLOGI

BIOLOVA ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. 87-95
Author(s):  
M Rustam - Rifai ◽  
Hening Widowati ◽  
Agus Sutanto

Abstract: Antagonistic bacteria in the activities of one organism with other organisms compete with each other in fighting over places, air, water, food (nutrients).  If the bacteria do not compete with each other but those bacteria can interact and synergize, and share the same nutritional sources and behave cooperatively between bacteria in their habitat. It is said to be synergisticThe goal is to find out the 15 consortia of LCN indigenous bacteria with soil bacteria can be synergistic so, it can be consortified. To utilize the synergistic test results of the consortia of LCN indigen bacteria with soil bacteria as a source of learning biology. The research design is a quantitative experiment . Synergistic test using Streak Plate Method on Nutrient Agar medium. The test results show that X2value 56.86> X2table 23.68 with α 0.05 in the chi-square table, so it can be concluded that there is a synergy between LCN Indigenous Bacteria and Soil Bacteria. The validation results show that the microbiology practicum guide with 96% results has a very good qualification so, it is suitable as a biology learning source in the form of a microbiology practicum guide based on scientific approach.  

2018 ◽  
Vol 6 (2) ◽  
pp. 47-52
Author(s):  
Suprapto Surapto ◽  
Tri Gunaedi ◽  
Basa T. Rumahorbo

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and  its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all  isolates were quite high: which were 35 577, 18 903,  32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.


2020 ◽  
Vol 5 (3) ◽  
pp. 875
Author(s):  
Asfri Sri Rahmadeni

<p class="Normal1"><em>Commitment is the attitude or behavior of likes or dislikes shown by someone against the organization at work. Indonesia is recorded as a nurse who has a low organizational commitment (76%) so it harms services. This phenomenon in the hospital can be seen from the indicator of the high turnover rate of nurses which reaches 30.9% which exceeds the standard &lt;5%. Many factors affect organizational commitment. The purpose of this study was to analyze the Personal Factors Associated with Organizational Commitment of Hospital Nurses. This study is a cross-sectional analytic study and purposive sampling technique with a sample of 59 nurses. Chi-Square test results obtained personal factors that have a relationship with organizational commitment Nurse Hospital is age with p = 0,000 and education with p = 0,000, personal factors not related to organizational commitment are gender p = 1,053 and years of service with p = 1,147. Hospitals should conduct research/surveys on Organizational Commitment periodically as an evaluation material in the context of the organization's development and development in the future</em>.</p>


Author(s):  
Y. Widyastuti Y. Widyastuti

  ABSTRACT [In advanced countries the number of permanent contraception option ( kontap ) approximately 10 % of all national family planning methods , while in Indonesia reached approximately 5.7 % . Based on data obtained from medical records Bhayangkara Hospital Palembang in 2010 the number of achievement when seen from the percentage of contraceptive use is only 9.10 % The purpose of this study is known age and parity relationship with the selection of contraceptive methods Surgery Women ( MOW ) in Palembang RS.Bhayangkara obstetrics clinic in 2012 . This study uses the analytic survey with cross sectional approach . The population in this study were women who become new acceptors were recorded in medical records at the hospital . Palembang Bhayangkara Hospitals January 1, 2012 to December 31 in 2012 . The sample in this study is part of a whole number of new acceptors ( either using contraception methods MOW and other family planning methods ) were registered in the hospital . Palembang Police Hospitals of January 1, 2012 to December 31 years of data 2012.Analisa univariate and bivariate statistical Chi - Square test with significance level α = 0.05 . The results showed there were 80 respondents ( 46.3 % ) who became MOW contraceptive acceptors , acceptors who have old age by 45 respondents ( 56.3 % ) and who has a young age by 35 respondents ( 43.8 % ) . acceptors which have high parity were 44 respondents ( 55 % ) who had low parity and a total of 36 respondents ( 45 % ) . From the results of Chi-square test value obtained ρ = 0.00 is smaller than α = 0.05 . There is a significant correlation between age and the selection of MOW Chi square test results obtained ρ value = 0.00 and there was a significant association between parity with the election MOW Chi square test results obtained ρ value = 0 , . From these results , it is expected to power health, especially family planning services at the hospital . Palembang Police Hospitals in order to improve the quality of family planning services in an effective, efficient , and safe are considered particularly suitable for users of contraception OperatingMethodWomen(MOW).                                      ABSTRAK Di negara sudah maju jumlah pilihan kontrasepsi mantap (kontap) sekitar 10% dari semua metode KB nasional, sedangkan di Indonesia baru mencapai sekitar 5,7 %. Berdasarkan data yang diperoleh dari Rekam medik  Rumah Sakit Bhayangkara Palembang tahun 2010 Jumlah pencapain tersebut jika dilihat dari persentasi penggunaan kontrasepsi MOW hanya  9,10% Tujuan penelitian ini adalah diketahuinya hubungan umur dan paritas dengan pemilihan alat kontrasepsi Metode Operasi Wanita (MOW) di poliklinik kebidanan RS.Bhayangkara Palembang Tahun 2012. Penelitian ini menggunakan metode survey analitik dengan pendekatan cross sectional. Populasi dalam penelitian ini adalah wanita yang menjadi akseptor KB Baru yang tercatat di rekam medik  di RS. Bhayangkara Palembang tanggal 1 januari 2012 sampai dengan 31 Desember  tahun 2012. Sampel dalam penelitian ini adalah sebagian dari seluruh jumlah akseptor KB Baru (baik yang menggunakan alat kontrasepsi Metode MOW maupun Metode KB lain) yang teregister di RS. Bhayangkara Palembang dari tanggal 1 januari 2012 sampai dengan 31 Desember  tahun 2012.Analisa data dilakukan secara univariat dan bivariat dengan uji statistik Chi-Square dengan tingkat kemaknaan α = 0,05. Hasil penelitian menunjukkan dari 80 responden terdapat (46,3%) yang menjadi akseptor kontrasepsi MOW, akseptor yang memiliki umur tua sebanyak 45 responden (56,3%) dan yang memiliki  umur muda sebanyak 35 responden (43,8%). akseptor yang memiliki paritas tinggi sebanyak 44 responden  (55%) dan yang memiliki  paritas rendah sebanyak 36 responden (45%). Dari hasil uji Chi square didapatkan ρ value = 0,00 lebih kecil dari α = 0,05. Ada hubungan yang bermakna antara umur dengan pemilihan MOW dari hasil uji Chi square didapatkan ρ value = 0,00 dan ada hubungan yang bermakna antara paritas dengan pemilihan MOW hasil uji Chi square didapatkan ρ value = 0,.Dari hasil penelitian ini, diharapkan kepada tenaga kesehatan khususnya pelayanan KB  di RS. Bhayangkara Palembang  agar dapat meningkatkan kualitas pelayanan KB secara efektif, efisien, dan aman yang dianggap cocok bagi penggunanya khususnya mengenai kontrasepsi Metode Operasi Wanita (MOW).    


2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Andi Sitti Nur Afiah ◽  
Fera The

Tuberculosis (TB) is a pulmonary disease caused by Mycobacterium tuberculosis. Globally in 2018 theestimated number of people affected by TB was estimated at 10.0 million population and 484,000 cases ofmultidrug-resistant TB (MDR-TB). This study aims to determine the correlation between microscopic testresults with RMT on TB and MDR-TB patients at RSUD Dr. H Chasan Boesoirie Ternate. This type of researchis analytical research using a retrospective approach. The sample in this study were patients with suspected TBand MDR-TB who had performed microscopic tests and TCM in February – April at 2020 in the ClinicalPathology Laboratory of RSUD Dr. H Chasan Boesoirie Ternate, who met the inclusion and exclusion criteria.Data were analyzed using the SPSS program analysis was carried out in stages, namely by univariate andbivariate using chi-square test. From 100 samples, the results of RMT examination with TB suspects were 30rifampicin sensitive samples with 2 rifampicin resistance and 5 rifampin sensitive samples for TB MDR-TBsuspects RMT examination results. Chi-square test results obtained the value of p = 000 (p <0.05). There is stilla significant difference between the microscopic test results with RMT in TB and MDR-TB suspect patients atRSUD Dr. H Chasan Boesoirie Ternate.


2019 ◽  
Vol 11 (1) ◽  
pp. 3-11
Author(s):  
Dr. Swarooparanil Patil ◽  
Dr. B.S. Keshava Prasad

The aim of the study was to evaluate the effect of different concentrations of cetrimide with or without EDTA solution on the microhardness and surface changes in root dentin. Single rooted human mandibular premolar teeth were decoronated and sectioned longitudinally. The root segments were horizontally embedded in autopolymerizing resin. These specimens were randomly divided into 6 experimental groups according to the irrigating solution used. The irrigating solutions used were 5% EDTA, 5%EDTA + 0.25% Cetrimide, 5%EDTA + 0.50% Cetrimide, 0.25% Cetrimide, 0.50% Cetrimide and distilled water. Each group was further divided into two subgroups. First subgroup specimens were employed for microhardness testing using vicker’s indenter. Second subgroup specimens were used to evaluate the surface changes of root dentin under stereomicroscope. Comparison of the mean microhardness of test groups was done using ANOVA followed by post hoc Tukey’s test. Pre- and post- treatment hardness values were compared using student paired t test. The data obtained from surface erosion scoring was subjected to statistical analysis using Chi square test. Results of this study showed significant reduction in the microhardness of dentin for all the groups except for the distilled water (p<0.001). The greatest reduction in dentin microhardness was observed with Group-3 (5% EDTA + 0.50% CTR). Specimens in 5% EDTA group showed greater erosion than other groups. Within the limitations of this study it was concluded that all the tested irrigating solutions reduced the microhardness of root dentin except distilled water. Maximum reduction in microhardness was observed with addition of 0.50% cetrimide to EDTA. EDTA increased the surface roughness of root dentin irrespective of cetrimide association. Use of cetrimide at concentrations higher than 0.25% would be detrimental to the microhardness of dentin.


1963 ◽  
Vol 9 (2) ◽  
pp. 187-197 ◽  
Author(s):  
E. C. S. Chan ◽  
H. Katznelson ◽  
J. W. Rouatt

These studies are concerned with the growth interrelationships of mixed cultures of five soil organisms in soil extract and root extracts of 2-, 4-, and 8-week-old oats, soybeans, and wheat. Population changes of Agrobacterium radiobacter, Arthrobacter citreus, Azotobacter chroococcum, Bacillus cereus, and a Pseudomonas sp. in pure and mixed culture were followed by plating on selective media. B. cereus and A. chroococcum grew poorly alone or in mixed culture in the extracts. In soil extract, A. citreus predominated over, or was nearly equal in number to, the Gram-negative forms (Pseudomonas and Agrobacterium). In root extracts, Pseudomonas sp. always predominated over A. citreus in mixed culture. A. radiobacter was inhibited in mature root extracts (8-week-old plants) although in pure culture it recovered after a period. An antagonistic effect of Pseudomonas sp. on A. chroococcum plated on nitrogen-free agar medium was found to be related to the kind of agar used.


2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Shubhangi S. Dere ◽  
Dr. Bindoo S. Jadhav ◽  
Dr. Dipak C. Patil ◽  
Dr. Hemangi S. Dhavale

Background: Schizophrenia is a clinical syndrome of variable, but profoundly disruptive, psychopathology that involves cognition, emotion, perception and behaviour. Caregiver burden refers to negative response that occurs when the caregiver assumes an unpaid and unanticipated responsibility for the person with disabling mental health problems. The burden varies with socio-demographic parameters of patients and caregivers. Aims & Objectives: To study socio demographic details of schizophrenia patients and caregivers. To assess perceived level of burden in caregivers and it’s association with socio demographic profile of patients and caregivers. Materials & Methods: Ethics committee approval and informed consent was taken. 100 consecutive patients with their primary caregiver, presenting to psychiatry OPD of general hospital, satisfying inclusion criteria, were included in the study. Socio-demographic details of caregiver were collected using semi-structured proforma. Caregiver’s perceived burden was assessed with Burden Assessment Schedule. Data thus obtained, was pooled & analyzed by using SPSS software, descriptive statistics, mean, percentage, Chi- Square test. Results: The sample consisted of 52 female & 48 male patients. Prevalence of burden in caregivers was found to be 52%. The perceived burden was found to be high in caregivers of female patients, patients of older age, less educated, single, and unemployed patients. Majority of caregivers were female and of older age. The burden perceived was found to be significantly high in female caregivers, especially mother.


Cocoons of earthworm Eudrilus eugeniae were collected from vermiculture bed and found that it had antibacterial activity. The size of zone of inhibition was directly proportional to the size of cocoons examined. Along with nutritious fluid and embryos, culturable bacterial community was found inside the cocoons. Bacterial colonies were isolated from the trails of newly hatched, juvenile worms in the nutrient agar medium and examined. Gram negative, rod shaped bacterium was found to be abundant in the trails of juvenile earthworms. Polymerase chain reaction was performed from this bacterium to amplify the gene of 16S rRNA and analyzed. Subsequent bi-directional DNA sequencing revealed that this abundant bacterium is highly related to 16S rRNA gene sequence of a strain, Alcaligenes faecalis. Based on available literature, we hypothesize that this bacterium could be symbiotically associated with cocoons of earthworms.


2021 ◽  
Vol 5 (1) ◽  
pp. 018-021
Author(s):  
Gómez Elena ◽  
Guzmán Marianela ◽  
Torres Oriol Yuguero

Introduction: Psychiatric emergencies constitute between 1% - 10% of general emergencies in Spain, of which a quarter will end up being hospital admissions. There is little literature on patients who are referred from general to specialized hospitals. Methods: Cross-Sectional study of all the patients referred in 2018. Sociodemographic variables and clinical variables, such as diagnosis and discharge destination, were analyzed. An analysis was performed by comparing means with the Chi Square test. Results: 433 derived emergencies were analyzed. Most of the patients were women. The main reason for derivation were suicide attempts. Of those patients, 40% required hospitalizations. More than 50% of the derived emergencies were referred to the home and followed up on an outpatient basis. Conclusion: Most derived emergencies are referred to the home after evaluation. This implies that most are not life-threatening and that with adequate tools for evaluating suicide risk, we could avoid transfers and improve the resolution of these cases.


2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Rahayu Setyaningsih ◽  
Kristiani Eka Prasetyo Wat ◽  
Asri Utami

AbstractIntroduction: Infant massage is a touch therapy oldest known to the public. Infant massage is one of the communication between mother and child is communication through touch. Infant massage has been known to man for a long time and reduced down - generations. Age 1-12 months is a period of rapid growth that is traversed by the child, including motoric development. Infant massage can provide a stimulus to promote weight and to increase motoric development.The purpose: Of this study was to correlate of infant massage and motoric development in infants aged 1-12 months in District Pundungsari Bulu Sukoharjo.The subjects: Were mother with infants aged 1-12 months, in the District Pundungsari. Sampling is done with total sampling technique. They are 33 sample in this research.Methods: This study was a non-experimental studies, correlation approach. Data obtained by the method of questionare, which is mother has filled the questionare about baby massage in infants aged 1-12 months and motoric development. The data have been collected and analyzed by chi square with p= 0.05. The research found that respondents do baby massage with continue and not continue and motoric development found delayed, normal and advance. After the test results obtained chi square p 0.000 to p <0.05, which means Ha accepted and Ho rejected.The conclusion: Of this study is infant massage correlate with motoric development in infants aged 1-12 months in District Pundungsari Bulu Sukoharjo.Keywords: Infant massage, motoric development


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