scholarly journals Tinjauan Pustaka : Teknik Pengujian Toksisitas Teratogenik Pada Obat Herbal

2020 ◽  
pp. 31
Author(s):  
Tri Mulyani ◽  
Cinta Ida Julianti ◽  
Rikkit Sihombing
Keyword(s):  
Toxicity Test ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Toxicity Testing ◽  
Toxicity Tests ◽  
Melia Azedarach ◽  
Testing Technique ◽  
General Toxicity ◽  
Bougainvillea Glabra ◽  
Dark Pink

Toxicity testing of natural ingredients used in raw materials for making herbal medicines needs to be done to ensure its safety. In general, toxicity tests are divided into 2 types namely general toxicity and specific toxicity. One specific toxicity test is the teratogenic test. Teratogenic testing is part of a special toxicity test that is intended to determine the toxic effect of a compound on the fetus or fetus. The writing of this journal aims to explain the teratogenic toxicity testing technique by referring to the Organization for Economic Co-operation and Development (OECD) 414 and OECD 236 on herbal medicines. Some examples of herbs used for testing teratogenic toxicity as studied by Lu E. The et al. On Bougainvillea glabra (Paper Flower) with the results of a study of water extracts in purple and dark pink, B. glabra bract is not toxic to embryos, except the pink type with LC 50 85.51 ?g / mL. then there is Mindi leaf (Melia azedarach L) which was investigated by Adisti erlina sutomo et al with the results of extracting at a dose of 3.22 mg / kg bw already causing teratogenic toxicity. And there are still some herbs that are used for teratogenic toxicity testing with mixed results.

scholarly journals WASTE TO PRODUCT: BISOLUM-BRICKS, INCORPORATION OF WWTP SLUDGE OF TEXTILE INDUSTRY INTO BRICKS FOR WALL PAIRS

2019 ◽  
Vol 10 (2) ◽  
pp. 29-35
Author(s):  
Armas Arifin Arbunowo ◽  
P. Purwanto Purwanto ◽  
M. Arief Budihardjo
Keyword(s):  
Toxicity Test ◽  
Textile Industry ◽  
Raw Materials ◽  
Quality Standards ◽  
Toxicity Tests ◽  
Acute Toxicity Test ◽  
Technical Requirements ◽  
The Republic ◽  
Industry Wastewater

The disposal of WWTP sludge is one of problems in textiles industry, which requires serious attention to find a way out. Utilization of sludge from the textile industry wastewater treatment, according to the Republic of Indonesia Goverment Regulation No.110 year 2014, can be used as a mixture of brick raw materials, must consider the availability of technology, meet environmental quality standards and meet technical requirements for use.Environmental feasibility refers to Goverment Regulation No.110 year 2014, carried out with TCLP toxicity tests on raw materials for soil, WWTP  sludge and brick products. Acute toxicity test LD 50, carried out on brick products in which  using a mixture of WWTP Sludge. Technical feasibility is carried out by testing the quality of brick products in accordance with solid brick Nasional Indonesian Standard (SNI) for wall pairs.Research results prove the toxicity test on raw materials and brick products with a mixture of up to 60% of waste, still meets the requirements of TCLP and LD.50 according to GR No.110 year 2014. Test the quality of bricks at the use of 40% and 60% mixture of sludge still meet Nasional Indonesian Standard (SNI 15-2094-2000) solid red brick for wall pairs.

Regulatory ecotoxicology testing in Canada – activities and influence of the Inter-Governmental Ecotoxicological Testing Group

2013 ◽  
Vol 48 (1) ◽  
pp. 14-29 ◽  
Author(s):  
Lisa N. Taylor ◽  
Kenneth G. Doe ◽  
Richard P. Scroggins ◽  
Peter G. Wells
Keyword(s):  
Toxicity Test ◽  
Ad Hoc ◽  
Toxicity Testing ◽  
Test Methods ◽  
Toxicity Tests ◽  
Environmental Programs ◽  
Ecotoxicity Testing ◽  
Guidance Documents ◽  
Government Laboratories ◽  
Testing Group

The Inter-Governmental Ecotoxicological Testing Group (IGETG) is an ad hoc group of government scientists, technologists, data users, and scientific advisors that has been active in the development and application of ecotoxicological testing in Canada. Membership includes representatives from government laboratories that conduct toxicity testing for research and development purposes, monitor effluent discharge for compliance with regulations, and/or perform exploratory monitoring of non-regulated sectors. The original focus of the group was to support the development and application of standardized toxicity test methods under the Fisheries Act but as the group matured it broadened its focus to five goals: (1) to promote the use of ecotoxicity testing; (2) to disseminate and harmonize new knowledge and understanding of issues related to ecotoxicity testing; (3) to provide scientific support to environmental programs; (4) to develop, validate and publish toxicological test methods; and (5) to establish and implement quality assurance practices in toxicology laboratories. Since 1990, IGETG has assisted Environment Canada in standardizing 22 toxicity test methods and in developing eight guidance documents. In this context, we briefly outline the history and future of applied ecotoxicological testing in Canada illustrated by specific examples wherein standard toxicity tests are useful. This paper commemorates IGETG's 35th anniversary.

Assessment of diclofenac LC50 reference values in juvenile and embryonic stages of the zebrafish (Danio rerio)

2011 ◽  
Vol 14 (4) ◽  
pp. 545-549 ◽  
Author(s):  
E. Praskova ◽  
E. Voslarova ◽  
Z. Siroka ◽  
L. Plhalova ◽  
S. Macova ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Reference Values ◽  
Danio Rerio ◽  
Toxicity Test ◽  
Statistical Significance ◽  
Juvenile Fish ◽  
Toxicity Testing ◽  
Probit Analysis ◽  
Model Organisms ◽  
Toxicity Tests

Assessment of diclofenac LC50 reference values in juvenile and embryonic stages of the zebrafish (Danio rerio) The aim of the study was to compare the acute toxicity of diclofenac to juvenile and embryonic stages of the zebrafish (Danio rerio). Acute toxicity tests were performed on the aquarium fish Danio rerio, which is one of the model organisms most commonly used in toxicity testing. The tests were performed using a semi-static method according to OECD guideline No. 203 (Fish, acute toxicity test). Embryo toxicity tests were performed in zebrafish embryos (Danio rerio) in compliance with OECD No. 212 methodology (Fish, short-term toxicity test on embryo and sac-fry stages). The results were subjected to a probit analysis using the EKO-TOX 5.2 programme to determine 96hLC50 and 144hLC50 (median lethal concentration, 50% mortality after a 96 h or 144 h interval, respectively) values of diclofenac. The statistical significance of the difference between LC50 values in juvenile and embryonic stages of Danio rerio was tested using the Mann-Whitney non-parametric test implemented in the Unistat 5.1 programme. The LC50 mean value of diclofenac was 166.6 ± 9.8 mg/L in juvenile Danio rerio, and 6.11 ± 2.48 mg/L in embryonic stages of Danio rerio. The study demonstrated a statistically higher sensitivity to diclofenac (P<0.05) in embryonic stages compared to the juvenile fish.

Acute Toxicity Testing with Juvenile White Sturgeon (Acipenser transmontanus)

1998 ◽  
Vol 33 (1) ◽  
pp. 95-110 ◽  
Author(s):  
W.R. Bennett ◽  
A.P. Farrell
Keyword(s):  
Acute Toxicity ◽  
Swimming Performance ◽  
Juvenile Fish ◽  
Toxicity Testing ◽  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Toxicity Tests ◽  
Fraser River ◽  
Acute Toxicity Tests ◽  
Acute Toxicity Testing

Abstract The primary goal of this study was to investigate the possibility of using early life stages of white sturgeon (Acipenser transmontanus) (eggs, larvae and fry) as a species relevant to the Fraser River, B.C., for the acute and sublethal toxico-logical testing of forest industry effluents. Here we report the first successful acute toxicity tests for 8-day-old larvae and 42-day-old fry exposed to several chemicals known to be released into the Fraser River (i.e., 6 monochlorovanillin [6 MVAN], 4,5 dichloroguaiacol [4,5 DCG], 4,5 dichlorocatechol [4,5 DCAT], pentachlorophenol [PCP], and didecyldimethylammonium chloride [DDAC]). In most cases, white sturgeon fry were at the lower end of the range for acute toxicity values for chlorinated phenolic compounds, when compared with other juvenile fish species, and they were extremely sensitive to DDAC. The larval stage was usually more sensitive than the fry stage. Acute toxicity tests with fertilized eggs were unsuccessful. A postexposure growth study was inconclusive because neither control nor toxicant-exposed larvae and fry withstood the additional handling used for measuring body mass. At 62-days-old, fry were more tolerant of handling. This allowed measurement of their swimming performance. Although we have concerns about the reliability of using larvae for acute toxicity testing at this time, 60-day-old white sturgeon fry would appear to be both a sensitive and relevant species for assessing environmental impacts relevant to the Fraser River.

Phamacognostical and Pharmaceutical Evaluation of Chandrashakaladi Vataka - A herbal formulation

2016 ◽  
Vol 1 (3) ◽  
Author(s):  
Dr. Akash S Changole ◽  
Mandip Goyal ◽  
Harish CR
Keyword(s):  
Classical Method ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Vital Role ◽  
Published Data ◽  
Herbal Formulation ◽  
Physico Chemical ◽  
Good Manufacturing Practices ◽  
Solvent Systems

Background: Quality control and the standardization of herbal medicines involve steps like standard source and quality of raw materials, good manufacturing practices and adequate analytical screening. These practices play a vital role in guaranting the quality and stability of herbal preparations. Chandrashakaladi Vataka is an Ayurvedic herbal formulation mentioned to be beneficial in Kushtha. Till date no published data is available on its analytical profile. Aim: To develop the Pharmacognostical and Phyto-chemical profile of Chandrashakaladi Vataka. Material and Methods: Chandrashakaladi Vataka was prepared as per classical method and analytical findings were recorded. Samples were subjected to organoleptic analysis, physico-chemical analysis and HPTLC examination by optimizing the solvent systems. Results and Conclusions: Pharmacognostical profile of Chandrashakaladi Vataka was established. Loss on drying, Ash value, Acid insoluble extract, Methanol soluble extract, Chandrashakaladi Vataka was found within prescribed limits. HPTLC profile of Chandrashakaladi Vataka revealed 12 spots at 254 nm and 13 spots at 366 nm.

scholarly journals Potential Use of the Benthic Foraminifers Bulimina denudata and Eggerelloides advenus in Marine Sediment Toxicity Testing

Water ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 775
Author(s):  
Mary McGann
Keyword(s):  
Sea Urchin ◽  
Marine Sediment ◽  
Sediment Toxicity ◽  
Toxicity Testing ◽  
Fertilization Success ◽  
Toxicity Tests ◽  
Sediment Chemistry ◽  
Benthic Foraminifers ◽  
Sediment Toxicity Tests ◽  
Unionized Ammonia

The benthic foraminifers Bulimina denudata and Eggerelloides advenus are commonly abundant in offshore regions in the Pacific Ocean, especially in waste-discharge sites. The relationship between their abundance and standard macrofaunal sediment toxicity tests (amphipod survival and sea urchin fertilization) as well as sediment chemistry analyte measurements were determined for sediments collected in 1997 in Santa Monica Bay, California, USA, an area impacted by historical sewage input from the Hyperion Outfall primarily since the late 1950s. Very few surface samples proved to be contaminated based on either toxicity or chemistry tests and the abundance of B. denudata did not correlate with any of these. The abundance of E. advenus also did not correlate with toxicity, but positively correlated with total solids and negatively correlated with arsenic, beryllium, chromium, lead, mercury, nickel, zinc, iron, and TOC. In contrast, several downcore samples proved to be contaminated as indicated by both toxicity and chemistry data. The abundance of B.denudata positively correlated with amphipod survival and negatively correlated with arsenic, cadmium, unionized ammonia, and TOC; E. advenus negatively correlated with sea urchin fertilization success as well as beryllium, cadmium, and total PCBs. As B. denudata and E. advenus are tolerant of polluted sediments and their relative abundances appear to track those of macrofaunal toxicity tests, their use as cost- and time-effective marine sediment toxicity tests may have validity and should be further investigated.

Study on the Safety of Mineral and Synthetic Oil Added Commonly Used Extreme Pressure Additives

2012 ◽  
Vol 430-432 ◽  
pp. 1386-1389
Author(s):  
Zhuo Jun Chen ◽  
Long Long Feng ◽  
Bao Liang Li ◽  
Jin Jin Yue ◽  
Ying Liang Wu ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Toxicity Test ◽  
Mineral Oil ◽  
Stimulation Test ◽  
Toxicity Tests ◽  
Acute Toxicity Test ◽  
Base Oil ◽  
Synthetic Oil ◽  
Four Levels ◽  
Oral Acute Toxicity

This article use the Sulphide Isobutene (T321), Five Sufides Dialkyl(RC2540) and Star of Phosphorus(P110) as the additives,Neopentyl Polyol Ester(NPE) and mineral oil N32 as base oil. Compound above additives and base oil for the four levels. A sample: adding 4% T321 additive in NPE. B sample: adding 4% T321 additive in N32. C sample: adding 4% RC2540 additive in NPE. D sample: adding RC2540, T321 and P110 additives in NPE (all is mass fraction). The oral acute toxicity test, eye mucous stimulation test, skin hypersensitive test, soaking tail toxicity tests were conducted in above samples. The test results show that. The mineral oil, it’s not only toxic then synthetic oil but also has a poor lubricating ability compare with the same percent additive in synthetic oil. In oral acute toxicity test, eye mucous stimulation test, skin hypersensitive test, soaking tail toxicity tests, Toxic reaction of mineral N32+4%wt Sulphide Isobutene (T321) obviously from other oil samples.

scholarly journals Survey and Documentation of Ethnobotanicals used in the Traditional Medicines System of Tribal Communities of Chirang District of Assam Against Helminthiasis

2019 ◽  
Vol 12 (04) ◽  
pp. 1923-1935
Author(s):  
Ananta Swargiary ◽  
Mritunjoy Kumar Roy ◽  
Manita Daimari
Keyword(s):  
Sex Education ◽  
Raw Materials ◽  
Herbal Medicines ◽  
Ananas Comosus ◽  
Asparagus Racemosus ◽  
Traditional Medicines ◽  
Face To Face ◽  
Plant Parts ◽  
Tribal Communities ◽  
Ancient Times

Ethnobotanical knowledge has been the backbone of rural healthcare since ancient times. Many diseases including helminthiasis are cured by traditional medicine in many parts of the world. The present study aims at exploring the ethnobotanicals used as anthelmintic medicines by the tribal communities of Chirang district of Assam. The present study was conducted in different villages under Chirang district of Assam, India. A face-to-face interview was carried out during survey work along with readymade questionnaire. In our survey work, 20 neighbouring villages were taken as a single cluster and one sample informant was collected. Information regarding the plant and plant parts used, methodology of use as well as informant demography such as age, sex, education was also collected. A total of 20 villages were surveyed and information was gathered from 27 informants, 23 kaviraja and 4 elderly people, 15 male and 12 female. The information collected revealed 43 medicinal plants belonging to 27 families. Lamiaceae was found to be most common family followed by Cucurbitaceae, Fabaceae, Zutaceae and Zingiberaceae. The most highly cited plants were Ananas comosus, Andrographis paniculata, Asparagus racemosus, Alstonia scholaris and Leucas aspera. Leaves, fruits and tubers were found to be the most commonly used plant parts. Except few, most of the herbal medicines were prepared as raw materials and are consumed orally. Documentation of important ethnomedicinal information from the remote areas of Assam will help scientific investigators to look into its scientific aspect leading to the development of new medicines against helminthiasis and many other diseases.

scholarly journals ANALISIS KANDUNGAN SAPONIN PADA DAUN DAN TANGKAI DAUN Carica pubescens Lenne & K. Koch

el–Hayah ◽  
2016 ◽  
Vol 5 (4) ◽  
pp. 143 ◽  
Author(s):  
Eko Budi Minarno
Keyword(s):  
Raw Materials ◽  
Herbal Medicines ◽  
The Body ◽  
Raw Material ◽  
Ring Test ◽  
Tropical Species ◽  
Color Test ◽  
Natural Medicine ◽  
Chemical Content ◽  
Carica Pubescens

<p><em>Carica pubescens Lenne &amp; K. Koch is a tropical species that adapt to the plateau environment and low temperatures. In East Java, the plant is found in Cangar and Bromo region. Morphological, chemical content, and analysis of protein banding pattern on C. pubescens has been done, but more on the analysis of active compounds for pharmaceutical raw materials and its accumulation in the body of the plant has not been widely studied. Saponins on C. pubescens potential as a raw material of natural medicine in the treatment of Diabetes Mellitus (DM). This study aims to determine the content of saponin in leaf and petiole of C. pubescens in terms of absorbance values. Saponins were analyzed by qualitative form the foam test, color test, Thin Layer Chromatography (TLC) analytical and preparative. Quantitative test in the form of UV-Vis spectrophotometry results preparative TLC. This research was done at the Laboratory of Department of Biology and Chemistry UIN Maulana Malik Ibrahim Malang. The results showed that the leaf and petiole of C. pubescens positive for the saponins with the formation of stable foam for 60 seconds at 1.5 cm - 1.7 cm. The third positive samples containing saponins triterpene the ring test produces a brownish color. Isolation saponin by TLC shows the best ratio of eluent chloroform: methanol: water (14: 6: 1) compounds can be separated perfectly. Saponin absorbance values obtained three samples as follows: petiole samples from the region Cangar amounted to 0.852, leaf samples from the region Cangar amounted to 0.686, and leaf samples from Bromo region amounted to 0,629. The highest saponins found in organs petiole. Thus the petiole of C. pubescens has the potential to be used as a source of triterpene saponins which can be developed into a commercial herbal medicines.</em></p>

scholarly journals AN EXHAUSTIVE REVIEW ON HERBS USED IN THE MANAGEMENT OF DIABETES MELLITUS TYPE 2

2019 ◽  
pp. 5-11
Author(s):  
Salman Mau ◽  
Shakir Saleem ◽  
Vishwadeepak Kimothi ◽  
Vineet Joshi ◽  
Sanjay Singh
Keyword(s):  
Diabetes Mellitus ◽  
Raw Materials ◽  
Nigella Sativa ◽  
Low Cost ◽  
Herbal Medicines ◽  
Analytical Techniques ◽  
Herbal Drugs ◽  
Herbal Products ◽  
High Blood Glucose ◽  
Glucose Levels

Diabetes mellitus is one of the most common metabolic disorders associated with disturbed hormonal secretion. Diabetes is characterized by high blood glucose levels over a prolonged period of time. High sugar levels are due to abnormal metabolism of carbohydrates and lipids which is caused by absolute or relative insulin deficiency. Herbal medicines have been the highly esteemed source of medicine throughout the human history. Herbs are becoming more popular today because of their least side effects, holistic beliefs, easy availability and low cost. Individual herbal products and formulations are gaining popularity because of their quality manufacturing using modern analytical techniques and standardized raw materials. Herbal drugs are widely used for the treatment of diabetes worldwide in various dosage forms. India has a long list of native herbal drugs with scientifically proven blood sugar lowering properties. The seeds of Nigella sativa, Olea europaea, fruits of Aegle marmelos, Momordica charantia, Coccinia indica, Nigella sativa,Gymnema sylvestre leaves,whole plant of Pterocarpus marsupium, Syzygium cumini fruits, Swertia punicea, Urtica dioica, gum of Ferula assa-foetida and seeds of Trigonella foenum graecum were discussed along with their reported mechanisms of action. In this review paper an attempt has been made to give an overview of certain Indian plants which have shown their anti-diabetic activity in various pre-clinical studies.

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