scholarly journals Primers application with the Tso31 gene target in the molecular identification of Taenia solium

2021 ◽  
Vol 2 (1) ◽  
pp. 35
Author(s):  
Nur Habibah ◽  
Heri Setiyo Bekti ◽  
Ni Wayan Rika Kumara Dewi ◽  
Luh Putu Rinawati ◽  
Burhannuddin Burhannuddin ◽  
...  

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.

2019 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Harfaina Harfaina ◽  
Suharyo Hadisaputro ◽  
Djoko Trihadi Lukmono ◽  
Mateus Sakundarno

Filariasis adalah penyakit infeksi yang disebabkan oleh cacing Wuchereria Bancrofti, Brugia Malayi, dan Brugia Timori yang menyebabkan cairan limfe tidak dapat tersalurkan dengan baik sehingga menyebabkan pembengkakan pada tungkai dan lengan. Meskipun tidak ada penyebab kematian tetapi menyebabakan cacat permanen dan stigma sosial. Eliminasi Filariasis dilakukan dengan Program Pengobatan Massal ke seluruh penduduk di daerah endemis setahun sekali selama 5 tahun. Keberhasilan program ini memerlukan kepatuhan minum obat pencegahan filariasis. Tujuan penelitian ini untuk mengetahui faktor-faktor yang mempengaruhi ketidakpatuhan minum obat sebagai upaya pencegahan filariasis. Penelitian ini Populasi dalam penelitian ini adalah penduduk berusia 15-65 tahun di dua kelurahan endemis yaitu kelurahan kuripan kertoharjo dan kelurahan jenggot selama mei-juli 2018. Sampel dalam penelitian ini 80 kasus dan 80 kontrol dengan teknik cluster random sampling. Variabel yang terbukti berpengaruh yaitu persepsi kerentanan negatif (OR=4,093) 95%CI=1,356-12,350 dan self efficacy negatif (OR=30,298) 95%CI=8,986-102,156. Persepsi kerentanan negatif dan self efficacy negatif merupakan faktor perilaku yang mempengaruhi ketidakpatuhan minum obat pencegahan filariasis. Diharapkan ada penelitian lanjutan tentang ketidakpatuhan minum obat pencegahan filariasis bukan berwujud persepsi tetapi dengan pengukuran faktor lingkungan sosial secara objektif dengan melakukan intervensi berupa perubahan perilaku.   Kata kunci : Filariasis, Ketidakpatuhan, Minum Obat, Mix Method   FACTORS THAT INFLUENCE DRINKING DRUG PREVENTION NON COMPLIANCE OF FILARIASIS IN PEKALONGAN CITY   ABSTRACT Filariasis is an infectious disease caused by worms Wuchereria Bancrofti, Brugia Malayi, and Brugia Timori, adult worm lives and damage reulting in blockage of lymph channels, causing swelling of the legs and arms. Although no cause of death but causes permanent disability and social stigma. Filariasis elimination done with the Mass Treatment Program to the entire population in endemic areas a year for 5 year. Succesfully this program required a medication adherence. The purpose of this study was to determine the factors that influence drug disobedience as an effort to prevent filariasis. This study uses a mix method. The population in this study were residents aged 15-65 years in two endemic villages, namely kuripan kertoharjo and jenggot villages during May-July 2018. Samples in this study were 80 cases and 80 controls with cluster random sampling technique. Variables that proved influential were perceptions of negative vulnerability (OR = 4,093) 95% CI = 1,356-12,350 and negative self efficacy (OR = 30,298) 95% CI = 8,986-102,156. Negative vulnerability perceptions and negative self efficacy are behavioral factors that influence non-compliance with filariasis prevention drugs. It is expected that further research on non-compliance with taking drugs to prevent filariasis is not a form of perception but objective measurement of social environmental factors by intervening in the form of behavior change.   Keywords: Filariasis, Noncompliance, Medication, Mix Method


2020 ◽  
Vol 3 (1) ◽  
pp. 11-20
Author(s):  
Siska Oktavia ◽  
Wahyu Adi ◽  
Aditya Pamungkas

This study aims to analyze the value of the density of marine debris, perceptions and participation in Temberan beach and Pasir Padi beach, as well as determine the relationship of perception and participation to the density of marine debris. This research is a type of research that is descriptive with a mixed approach (quantitative and qualitative). The study was conducted at Temberan beach in Bangka Regency and Pasir Pasir Beach Pangkal Pinang in October 2019. The sampling technique used was random sampling and purposive sampling. The data collection technique was carried out using observation technique namely sampling and questionnaire. The validity test uses the Pearson Product Moment formula and the reliability test uses the Cronbach’s Alpha formula. The results showed that the density of debris in the Temberan beach was more dominant at 10.92 pieces/meter2, while at Temberan beach 3 pieces/meter2. The results of perception and participation are different, with the Temberan beach occupying more complex waste problems. The relationship of perception and participation in the density of marine debris have a relationship that affects each other.


Author(s):  
Muhamad Afandi ◽  
Sari Yustiana

The purpose of this study is mainly to describe the performance of primary school teachersin the unit of education technical implementation in Banyumanik, Semarang. This researchwas conducted in January- August 2017. The sample of this research is 162 primaryschool teachers selected by random sampling technique in order to get the same opportunityas sample. The instruments used in this study were the assessment sheets, the RatingSheet consisted of 64 items of question, and 3 indicators of Primary School TeacherPerformance. The results show that each of Implementation of lesson plans is categorizedvery well (90, 9%), the execution of the learning is 83.4%, assessment of learning is good(82.8%). The Overall performance of primary school teachers is categorized good( 85 , 7 % ). In other words, the primary school teachers of the unit of education technicalimplementation in Banyumanik Semarang can arrange the lesson plans, carry out learningand teaching process and assess the learning process in elementary school.


Jurnal Ecogen ◽  
2019 ◽  
Vol 2 (4) ◽  
pp. 788
Author(s):  
Syawirda Syawirda ◽  
Yulhendri Yulhendri

The research aims to determine the effect of learning intensity and social capital to result of learning of students at state vocational high school 2 of Pariaman in academic year 2019/2020. The population in this research was all students of class XI until XII. The sample use are 107 with proportional random sampling technique. The analytical method used is multiple regression analysis using the SPSS version 20 program. This research use asosiatif method with deskriptive analysis. The data is analyted by using multiple regression. The result show that 1) There were a learning intensity and social capital to results of  learning of students (F sig = 0,000 < dari 0,05). 2) There was a effect between learning intensity to result of  learning (sig = 0,001< 0,05). 3) There was a effect between social capital to result of  learning (sig = 0,000< 0,05).   Keywords: intensity of learning, social capital, result of learning


2019 ◽  
Vol 118 (7) ◽  
pp. 82-94
Author(s):  
DR.C. KATHIRAVAN ◽  
DR.M. MANIVANNAN ◽  
E.CHANDRA MOULI ◽  
A. RAJASEKAR

The data were collected using personal interview method and a total of 455 employees who were in some aspect knowledge management in banks and identified through multistage random sampling method. Multistage random sampling technique is a probability sampling type where available study topics employ future topics from among their contacts. The study was limited to Chennai city of Tamil Nadu. The analysis found that banks employees moderately perceived towards factors of knowledge management such as knowledge utilization, information technology, knowledge motivation, knowledge storage, knowledge sharing enablers and knowledge creation. Hence, it is concluded that training program is important for the survival of knowledge management. It is also imperative for effective of employees’ job performance.


Author(s):  
Abdul Basith ◽  
Rosmaiyadi Rosmaiyadi ◽  
Susan Neni Triani ◽  
Fitri Fitri

The aim of this research is; 1) investigating the level of online learning satisfaction among students during COVID 19; 2) analyzing the influence of differences in gender, years of study, major in determining online learning satisfaction among students during COVID 19; 3) to analyze the relationship between online learning satisfaction and student academic achievement during COVID 19. The population was 656 students at STKIP Singkawang, and then a sample of 357 students (87 males and 270 females) was taken using a simple random sampling technique. The instrument in this study was adapted from Aman's Satisfaction instrument, which was then used to collect research data. Data analysis using SPSS with descriptive statistical techniques, MANOVA, and correlation. The results showed that online learning satisfaction was at a high level, meaning that students were satisfied with the online learning that had been implemented. The major differences have a significant effect on determining online learning satisfaction. Intercorrelation shows that there is a significant relationship on each indicator of online learning satisfaction with academic achievement, meaning that the higher the satisfaction felt by students in online learning, the student's academic achievement will increase.


Author(s):  
Khaulah Afifah ◽  
Lala M Kolopaking ◽  
Zessy Ardinal Barlan

Head of a village election with e-voting system is a new thing for community The success level of e-voting system can be reached by fulfil several principles in order to the implementation going effective and the result of the election can be accepted by all. The objectives of this research is to analyze the relation between the success level of e-voting system with social capital of the community. This research is carried out with the quantitative approach and supported by qualitative data. This research takes 60 respondents using simple random sampling technique. The results showed that the success level of e-voting has a correlation with the level of social capital of the community. Based on the field study, the social capital of the community is classified as high. The high social capital makes the implementation of e-voting successful and the success level is also high, because in the election ten years ago occurred a conflict. The community considers e-voting easier and more practical, cost effective and time-saving, and the results of e-voting are also reliable. A practical and fast of e-voting system can be a solution especially for “rural-urban” community who are busy or work outside the village.Keywords: E-voting, the success level of the system, social capital Pemilihan kepala desa dengan sistem e-voting merupakan hal yang baru bagi masyarakat. Keberhasilan penerapan sistem e-voting dilihat dari terpenuhinya beberapa prinsip agar penerapannya berlangsung efektif dan hasilnya dapat diterima oleh seluruh masyarakat. Penelitian ini bertujuan untuk menganalisis hubungan tingkat keberhasilan sistem e-voting dalam pemilihan kepala desa dengan tingkat modal sosial masyarakat. Bentuk penelitian ini adalah penelitian kuantitatif yang didukung oleh analisis data kualitatif. Penelitian ini mengambil enam puluh responden dengan teknik simple random sampling. Hasil penelitian menunjukkan bahwa tingkat keberhasilan e-voting memiliki hubungan dengan tingkat modal sosial masyarakat. Berdasarkan kajian di lapang, modal sosial masyarakat tergolong tinggi. Tingginya modal sosial tersebut membuat pelaksanaan e-voting berhasil dan tingkat keberhasilannya juga tergolong tinggi karena pada pemilihan sepuluh tahun silam sempat terjadi konflik. Masyarakat menganggap sistem evoting lebih mudah dan praktis, hemat dalam segi biaya dan waktu, serta hasil dari pemilihan juga dapat dipertanggungjawabkan. Sistem e-voting yang praktis dan cepat dapat menjadi solusi khususnya bagi masyarakat daerah “desa-kota” yang memiliki kesibukan atau pekerjaan di luar desa.Kata Kunci: E-voting, keberhasilan sistem, modal sosial. 


Author(s):  
Nyimas Ayu Dillashandy ◽  
Nurmala K Panjaitan

Mount Merapi eruption has occurred several times in Indonesia and the biggest eruption that last occurred in 2010. The community were suffered losses and were affected by eruptions. The purposes of this research are to analyze community resilience, to analyze the level of vulnerability, and to analize the community adaptive capacity. The research using a quantitative approach supported by qualitative data. Simple random sampling technique is used as the sampling method and the informant was taken purposively. The results of this research showed that when the eruption occurred the community has a high vulnerability. The adaptive capacity is also high with innovative learning based on institutional memory and supported by the connectedness. Communities achieve resilience and can adapt to changes with high adaptive capacity.  Keywords: adaptive capacity, community resilience, eruption, vulnerability ABSTRAK Erupsi Gunung Merapi sudah terjadi beberapa kali di Indonesia dan erupsi terbesar yang terjadi terakhir kalinya yaitu pada tahun 2010. Komunitas mengalami berbagai kerugian dan terkena dampak dari erupsi. Tujuan dari penelitian ini adalah untuk menganalisis resiliensi komunitas, menganalisis tingkat kerentanan komunitas, dan menganalisis kapasitas adaptasi komunitas. Penelitian ini dilaksanakan dengan menggunakan pendekatan kuantitatif yang didukung oleh data kualitatif. Pemilihan responden dilakukan dengan teknik sampel acak sederhana sedangkan pemilihan terhadap informan dilakukan secara sengaja. Hasil penelitian ini menunjukkan bahwa saat erupsi terjadi komunitas memiliki kerentanan yang tinggi. Kapasitas adaptasi komunitas tinggi dengan adanya innovative learning yang didasari oleh pengetahuan dan pengalaman dan didukung oleh jaringan yang dimiliki. Komunitas berhasil mencapai resiliensi dan dapat beradaptasi dengan perubahan-perubahan dengan kapasitas adaptasi yang tinggi.Kata kunci : kapasitas adaptasi, kerentanan, erupsi, resiliensi komunitas


Author(s):  
Supardi Supardi

<p>The purpose of this study was to determine the influence of learning discipline <br />and logical mathematical intelligence on mathematics achievement. This study is a survey design. This research did in student class XI IPA SMA Negeri 98 Jakarta semester 2012/2013. The sample taken by simple random sampling technique, involved 40 students. The instrument were in the forms of questionnaires and tests. The data were analyzed first by test requirements, the normality test, linearity and multicollinearity. Based on the fulfillment of the criteria in terms of the tes analyzed, inferential analysis to the test the research hypothesis. The data were analyzed using correlation and multiple regression. The findings showed that (1) learning discipline and logical mathematical intelligence had a positive and significant simultaneous impact on mathematics achievement. (2) learning discipline had a positive and significant impact on mathematics achievement. (3) logical mathematical intelligence had a positive and significant impact on mathematics achievement. </p>


2020 ◽  
Author(s):  
Pahrizal

The research is proposed to test the influence of Leadership and Motivation Work on Public Service Quality. The population in this study was the Kantor Kementerian Agama Sungai Penuh who had received the last 1 year service at the Kantor Kementerian Agama Sungai Penuh, amounting to 260 people. Furthermore, by using the Slovin formula, a sample size of 158 people was chosen with a sampling technique based on Simple Random Sampling. This study uses primary data and secondary data. Data analysis techniques used are path analysis and hypothesis testing using t test with data analysis using SPSS version 20. The results of the study based on showing that partially the Leadership and motivation work have a positive and significant effect on Public Service Quality. The results also show that motivation work variables act as intervening variables between Leadership and Public Service Quality.


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