scholarly journals Nil Tilapyası, Oreochromis niloticus (Linnaeus, 1758) Karaciğer Dokusunda Kurşunun Neden Olduğu Oksidatif Strese Karşı Humik Maddelerin Koruyucu Etkisi

2018 ◽  
Vol 6 (8) ◽  
pp. 1015
Author(s):  
Ferbal Özkan Yılmaz ◽  
Zülfiye Su ◽  
Arzu Özlüer-Hunt ◽  
Metin Yıldırım ◽  
Serap Yalın
Keyword(s):  
Lipid Peroxidation ◽  
Humic Substance ◽  
Oreochromis Niloticus ◽  
Liver Tissue ◽  
Lead Nitrate ◽  
Control Group ◽  
Fish Feed ◽  
Sod Activity ◽  
Antioxidant Enzyme System ◽  
Pb Concentration

In this study, the protective effect of humic substance in the fish feed was investigated against the effects of subletal lead (Pb) concentration on the antioxidant enzyme system in Oreochromis niloticus. For this purpose, the fish which were affected by 1.5 mg/L lead nitrate Pb(NO3)2 medium concentration were fed with feed containing 0.2% of humic substance. Liver samples were taken at 4th and 10th days in this study, which included control group, humic substance (HM), Pb, and Pb + HM groups. The enzyme activity of liver tissue catalase (CAT) and superoxide dismutase (SOD) of O. niloticus and the level of malondialdehyde (MDA), a product of lipid peroxidation, increased significantly at 4th and 10th days in the group with subletal lead concentration compared with the control group. Compared with Pb and HM+Pb groups, it was determined that humic substance application decreased Pb toxicity on lipid peroxidation of liver tissue and did not cause a significant change in CAT and SOD activity. According to the results of the experiment, 0.2% humic substance added to the feed reduced toxicity of subletal Pb concentration on liver tissue lipid peroxidation.

scholarly journals The effect of bee venom and hyperthermia on the lipoperoxidation and antioxidant activity of the blood of tumor-bearing animals

2021 ◽  
pp. 25-33
Author(s):  
M.A. Shabalin ◽  
◽  
A.V. Deryugina ◽  
V.V. Nazarova ◽  
E.A. Gracheva ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Malignant Tumors ◽  
Combined Treatment ◽  
Bee Venom ◽  
Control Group ◽  
Sod Activity ◽  
Tumor Bearing ◽  
Prolonged Effect ◽  
Control Tumor

Introduction. Nowadays, it has been shown that one of the possible ways to increase the effectiveness of the treatment of malignant tumors is the use of combined treatment methods. Aim. To study the intensity of lipid peroxidation (LPO) and the activity of superoxide dismutase (SOD) in hyperthermia against the background of bee venom in the blood of tumor-bearing rats. Materials and methods. The experimental animals were divided into 5 groups: 1st — intact; 2nd — control (tumor-bearing animals (PS-1 tumor strain) with intraperitoneal (IP) administration of saline solution); 3rd, 4th and 5th groups — tumor-bearing animals with IP administration of 0.5 ml of bee venom against the background of hyperthermia 42, 43 and 44°C respectively. The content of diene and triene conjugates, Schiff bases (SB) and SOD activity in the blood of animals was determined. Results. From the 1st day after the end of the experiment, a statistically significant decrease in triene conjugates was recorded in all experimental groups compared to the control group. SB decreased on the 1st–7th day after the end of the experiment with the action of hyperthermia 42°C and bee venom, on the 7th–28th day — with the action of hyperthermia 43°C and bee venom, and on the 28th day — with the action of bee venom and hyperthermia 44°C, which was accompanied by an increase in SOD activity from the 7th day in all experimental groups compared to the control group. Conclusion. Hyperthermia in combination with the action of bee venom causes a decrease in lipid peroxidation products and an increase in antioxidant activity in the blood of tumor-bearing rats. The most effective action, in our opinion, is the use of bee venom against the background of hyperthermia of 43°C, at which a prolonged effect is recorded both with respect to a decrease in the concentration of SB and an increase in the activity of SOD.

scholarly journals ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПОЛ ТА АКТИВНІСТЬ СИСТЕМИ АНТИОКСИДАНТНОГО ЗАХИСТУ У КРОВІ КОРОПІВ ЗА ДІЇ РІЗНОГО РІВНЯ ВІТАМІНУ Е І СЕЛЕНУ В РАЦІОНІ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 201-204
Author(s):  
S.V. Yurchak ◽  
O.V. Derenj ◽  
O.I. Vishchur ◽  
Yu.M. Zabytivskyi
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin E ◽  
Control Group ◽  
Fish Feed ◽  
Dose Dependent Decrease ◽  
Fat Soluble Vitamins ◽  
And Control ◽  
Dose Dependent ◽  
Experimental Group ◽  
Different Levels

The article consist data about effect of different levels of vitamin E and selenium in the diet of carps during their growing and also informationabout the influence on processes of lipid peroxidation and activity of antioxidant protection in their body.The experiment conducted in three experimental ponds. After wintering there were placed four ears mature females and males carp, six individuals in each group. Supplements of vitamin E administered at a rate of 25 mg/kg and drug «Sel–Plex» the rate of selenium – 0.3 mg/kg were added to further basic diet(BD) of female and male carp first experimental group (EG1). The second experimental group (EG2) received (BD) and vitamin E supplements in an amount of 75 mg / kg, and just as in EG1 – selenium – 0.3 mg/kg of feed.The control group received fish feed without additives vitamins and minerals.Feeding lasted for 30 days, due to the physiological needs of the fish feed. After spawning in fish of research and control group swere taken blood samples for biochemical research.The study led to a dose–dependent decrease (p < 0.01 – 0.001) content of TBA–active products and hydroperoxidase of lipids, but did not significantly effect onsuperoxide dismutase and glutathione peroxidase activity of blood So, the reduction of lipid peroxidation products in the carp’s blood of experimental groups probably was caused by the growth of non–enzymatic level, which is associated with fat–soluble vitamins. 

Protective effects of erdosteine on rotenone-induced oxidant injury in liver tissue

2004 ◽  
Vol 20 (6-10) ◽  
pp. 141-147 ◽  
Author(s):  
Alpaslan Terzi ◽  
Mustafa Iraz ◽  
Semsettin Sahin ◽  
Atilla Ilhan ◽  
Nuri Idiz ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Liver Tissue ◽  
Antioxidant Properties ◽  
Albino Rats ◽  
Protective Agent ◽  
Protective Effects ◽  
Oxidant Injury ◽  
Sod Activity ◽  
Significant Difference ◽  
Wistar Albino Rats

Rotenone, an insecticide of botanical origin, causes toxicity through inhibition of complex I of the respiratory chain in mitochondria. This study was undertaken to determine whether rotenone-induced liver oxidant injury is prevented by erdosteine, a mucolytic agent showing antioxidant properties. There were four groups of Male Wistar Albino rats: group one was untreated as control; the other groups were treated with erdosteine (50 mg/kg per day, orally), rotenone (2.5 mg/mL once and 1 mL/kg per day for 60 days, i.p.) or rotenone plus erdosteine, respectively. Rotenone treatment without erdosteine increased xanthine oxidase (XO) enzyme activity and also increased lipid peroxidation in liver tissue P < 0.05). The rats treated with rotenone plus erdosteine produced a significant decrease in lipid peroxidation and XO activities in comparison with rotenone group PB / 0.05). Erdosteine treatment with rotenone led to an increase in catalase (CAT) and superoxide dismutase (SOD) activities in comparison with the rotenone group PB / 0.05). There was no significant difference in nitric oxide (NO) level between groups. There were negative correlations between CAT activity and malondialdehyde (MDA) level (r= -0.934, P <0.05) with between CAT and SOD activities (r= -0.714, P <0.05), and a positive correlation between SOD activity and MDA level (r= 0.828, P <0.05) in rotenone group. In the rotenone plus erdosteine group, there was a negative correlation between XO activity and NO level in liver tissue (r= -0.833, P -0.05). In the light of these findings, erdosteine may be a protective agent for rotenone-induced liver oxidative injury in rats.

scholarly journals Effect of lipopolysaccharide (LPS) on the antioxidant enzyme system activity of erythrocytes and phagocytes of carps in in vivo studies

2018 ◽  
Vol 74 (1) ◽  
pp. 54-58
Author(s):  
ANTONINA SOPIŃSKA ◽  
ANNA GROCHOŁA
Keyword(s):  
Antioxidant Enzyme ◽  
Catalase Activity ◽  
In Vivo Studies ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Phagocytic Cells ◽  
Sod Activity ◽  
Antioxidant Enzyme System ◽  
Entire Experiment

The purpose of this experiment was to estimate the activity of antioxidant enzymes (SOD, GPx, CAT) in erythrocytes and phagocytes of carps (Cyprinus carpio L.) after intraperitoneal injection of LPS at a dose of 75 μg/100 g b.w. Enzyme activity was determined 3, 6 and 12 hours, as well as 3, 7, 14 and 28 days after LPS administration. After 3 and 6 hours, SOD in erythrocytes increased, respectively, to 188% and 142% of its control group level, and after 12 hours, SOD activity was significantly reduced (117%) and remained unchanged until the end of the experiment. From 12 hours after LPS administration until the end of the study, the PGx level was statistically significantly lower than in the control group, whereas the catalase activity was statistically significantly lower than in the control group in all study periods. In kidney phagocytic cells, SOD activity after 12 hours and 3 days was similar to that in the control group, and in the following study periods it amounted to 66-78% of the control values. Until the 14th day of observation, PGx activity was statistically significantly lower than it was in the control group. Catalase activity in kidney leukocytes was statistically significantly lower than in the control group during the entire experiment, and the lowest in the first study days, amounting to 48-42% of the control group value. The results indicate a long-term decrease in antioxidant enzyme activities in the experimental fish (lasting 14 or 28 days)...

Effect of Soil Pb Pollution on Physiological-Biochemical Characteristics of Young Tilia amurensis Rupr.

2011 ◽  
Vol 183-185 ◽  
pp. 1395-1399
Author(s):  
Wen Yuan Xu ◽  
Li Qiang Mu ◽  
Long Sun ◽  
Zhi Xin Wang
Keyword(s):  
Chlorophyll Content ◽  
Peroxidase Activity ◽  
Enzyme System ◽  
Small Degree ◽  
Control Group ◽  
Chloroplast Structure ◽  
Sod Activity ◽  
Biochemical Characteristics ◽  
Tilia Amurensis ◽  
Pb Concentration

Three-year old Tilia amerensis Rupr. was taken as study subject in a pot experiment. Chlorophyll content, REC, peroxidase activity, SOD activity, and other indices of Tilia amerensis Rupr. leaves were assayed. The experimental results show that Pb contaminated and destroyed the chloroplast structure of Tilia amerensis Rupr. leaves and impeded the synthesis of chlorophyll in the leaves. When Pb concentration was 10 mg•kg-1–100 mg•kg-1, REC of the Tilia extract was roughly equal to that of the control group, and Tilia amerensis Rupr. leaves were contaminated by Pb to a small degree. When Pb concentration reached a higher level, REC increased rapidly and Tilia amerensis Rupr. leaves were contaminated substantially. Low Pb concentration may lower the POD activity of the leaves. However, when Pb concentration was high, the POD activity elevated and Tilia amerensis Rupr. could exhibit various reactions to instinctively defend itself. The SOD activity of the Pb-contaminated Tilia amerensis Rupr. was slightly higher than that of the control group, and the ROS content remained low. The SOD enzyme system was undamaged.

scholarly journals The effect of curcumin on lipid peroxidation and selected antioxidants in irradiated rats

2018 ◽  
Vol 87 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Mehtap Ozcelik ◽  
Mine Erişir ◽  
Osman Guler ◽  
Murat Baykara ◽  
Esra Kirman
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Body Weight ◽  
Glutathione Peroxidase ◽  
Control Group ◽  
Sod Activity ◽  
Irradiation Group ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The effect of irradiation on oxidants and antioxidants in selected tissues and a possible protective effect of curcumin on these indices were investigated. A total of 28 rats were divided into 3 groups; group 1 was control; group 2 was the irradiation group, saline was administered intraperiotenally (i.p.) for three days and then, 9 Gy gamma irradiation was applied; group 3 was the irradiation + curcumin group: curcumin was given i.p. for three days at 200 mg/kg body weight and then the same dose of irradiation was applied. A significant increase in malondialdehyde (MDA) was detected in the liver, kidney, and brain tissues of the rats as a result of irradiation (P< 0.01). Glutathione peroxidase (GSH-Px) activity in all the tissues (except for kidneys) decreased (P< 0.01), liver SOD (superoxide dismutase) activity decreased (P< 0.05), and GSH (glutathione) levels in kidney and ovary tissues (P< 0.001) significantly increased. While curcumin administration returned the increased MDA levels in the kidneys and brain in result of irradiation to normal (P< 0.01), it did not return the increased MDA levels in the liver tissue to normal (P< 0.001) despite significantly reducing them. While decreased GSH-Px and SOD activity in the liver in result of irradiation increased with the addition of curcumin (P< 0.05), increased GSH levels in the kidneys and ovaries returned to control levels (P< 0.001). When MDA values were examined, it was found that the addition of curcumin protected the liver, kidneys and brain from the oxidative damage caused by irradiation.

scholarly journals Pengaruh pemberian kecambah kacang hijau (Phaseolus radiatus (L.)) terhadap kadar malondealdehid (MDA) plasma dan jaringan hati tikus Sprague Dawley yang diberi pakan lemak tinggi

2016 ◽  
Vol 13 (2) ◽  
pp. 82
Author(s):  
Novidiyanto Novidiyanto ◽  
Arta Farmawati ◽  
Lily Arsanti Lestari
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Level ◽  
High Fat Diet ◽  
Mung Bean ◽  
Liver Tissue ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

Background: High-fat diet increases the levels of fat, especially cholesterol and triglycerides that cause hyperlipidemia. Polyunsaturated fatty acids are very easily oxidized by free radicals reactive oxygens species (ROS) called lipid peroxidation, to produce compounds that malondialdehyde (MDA), is toxic and can damage plasma membrane and liver tissue. Mung bean sprouts are known to contain antioxidants which act to inhibit lipid peroxidation process.Objective: Determine the effect of mung bean sprouts to the level of plasma MDA and liver tissue of rats with high-fat diets.Method: This research is an experimental study with an only post-test design with the control group. Thirty-five male Sprague-Dawley rats were divided into 5 groups. Group K1 received fed standard; K2 received high-fat diet without mung bean sprouts. Group P1 received high-fat diet and mung bean sprouts dose of  0.5 mL/gBW. Group P2 received high-fat diet and mung bean sprouts dose of 1 mL/gBW. Group P3 received high-fat diet and vitamin E doses of 23 IU. All group were treated for 28 days. Analysis of MDA plasma and liver tissue performed after treatment.Results: Group K1 has plasma MDA levels of 1.17 ± 0.14 nmol/mL Mean MDA plasma level in the group K1 Group of K1 Mean MDA plasma level in the group K2 (39.7±0.19 nmol/mL) was higher than the group of K1 (1,17±0,14 nmol/mL), group P1 (2.94±0.09 nmol/mL), group P2 (1.73±0.08 nmol/mL) and group P3 (1.53±0.07 nmol/mL). Group K1 has liver tissue MDA Levels of 1,64±0,11 nmol/g. Mean MDA liver tissue level in the group of K2 (4.90±0.29 nmol/g) was higher than the group of P1 (3.68±0.45 nmol/g), group P2 (2.27±0.10 nmol/g), and group P3 (2.01±0.11 nmol/g).Conclusion: Level of MDA plasma and liver tissue of rat in group P1 and P2 was lower than a level of MDA plasma and liver tissue of rat in group K1, but higher than the level of MDA plasma and liver tissue of rat in group P3. There was a very strong correlation between the level of MDA plasma and liver tissue of rat was given the mung bean sprouts and high-fat diet.

scholarly journals The protective effect of crocin on cisplatin-induced testicular impairment in rats

BMC Urology ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Behzad Mesbahzadeh ◽  
Mohammadmehdi Hassanzadeh-Taheri ◽  
Mohadese-sadat Aliparast ◽  
Pardis Baniasadi ◽  
Mehran Hosseini
Keyword(s):  
Lipid Peroxidation ◽  
Testosterone Level ◽  
Control Group ◽  
Testis Weight ◽  
Testicular Toxicity ◽  
Germinal Layer ◽  
Sod Activity ◽  
Beneficial Effects ◽  
Male Wistar Rats ◽  
Testicular Impairment

Abstract Background Side effects of cisplatin (CIS) such as testicular toxicity restrict its clinical use. Instead, evidence indicates that crocin (CR) has synergistic anti-cancer potential with CIS and exhibited beneficial effects on CIS-induced hepatorenal damage. The aim of this study was to investigate the protective potential of CR against CIS-induced testicular toxicity in rats. Methods Fifty adult male Wistar rats randomly assigned to five equal groups including control, CIS, and CIS plus CR at doses of 6.25 mg/kg (CIS + CR6.25), 25 mg/kg (CIS + CR25), and 100 mg/kg (CIS + CR100). CIS and CIS + CR groups received a single intraperitoneally (i.p.) injection of CIS (7 mg/kg). CR (6.25–100 mg/kg i.p.) injections were started three days before the CIS injection and continued once a day for up to 13 days. On the 14th day, all animals were sacrificed and their blood samples and testes were removed for biochemical and histological analyses. Results Compared to the control group, CIS significantly decreased relative testis weight (0.28 vs. 0.39, p < 0.001), testosterone level (0.3 vs. 2.31 ng/mL, p < 0.001), germinal layer area (25,886 vs. 35,320 µm2, p < 0.001), superoxide dismutase (SOD) (0.9 vs.1.73 U/mg, p < 0.001) and increased testicular lipid peroxidation (3.05 vs. 15.35 nmol/mg, p < 0.001). CR at 25 mg/kg ameliorated testicular lipid peroxidation and enhanced SOD activity compared to CIS group (p < 0.05). Besides, CR treatment at the maximum dose (100 mg/kg) resulted in reversing CIS effects on testis weight, testosterone level, SOD, lipid peroxidation, and germinal layer area. Conclusions These findings demonstrated that CR co-treatment could prevent CIS-induced testicular toxicity in rats.

Evaluation of selected parameters of oxidative stress in patients with psoriasis

2016 ◽  
Vol 52 (2) ◽  
pp. 101-106
Author(s):  
Marta Pawłowska ◽  
Celestyna Mila-Kierzenkowska ◽  
Agnieszka Kwiatkowska ◽  
Jarosław Paprocki ◽  
Paweł Sutkowy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Venous Blood ◽  
Plasma Concentrations ◽  
Thiobarbituric Acid ◽  
Significant Degree ◽  
The Body ◽  
Control Group ◽  
Secondary Products ◽  
Sod Activity

Reactive oxygen species (ROS) are essential for proper functioning of the body. It is important to strike a balance between the production of ROS and their removal by the action of the antioxidant system. The deterioration of this balance may lead to oxidative stress, which causes damage to cell components of all – of lipids, proteins and DNA. Lipid peroxidation is a chain and free-radical process of lipid oxidation, in the course of which are involved ROS. The lipid peroxidation may play an important role in the pathogenesis of psoriasis. The aim of the study was to determine the concentration of thiobarbituric acid reactive substances, malondialdehyde and conjugated dienes, and the activity of superoxide dismutase in patients with psoriasis. Material and methods: The study group consisted of 12 women and 24 men with psoriasis – patients of the Department of Dermatology, Sexually Transmitted Infections and Immunodermatology CM UMK. The control group consisted of 16 healthy volunteers. The material for the study was venous blood. The concentration of TBARS and CD was determined in plasma and erythrocytes. The concentration of MDA was determined in plasma and SOD activity in the erythrocytes. Results: Statistically significantly higher concentration of MDA and statistically significantly higher concentration of TBARS was revaled in patients with psoriasis as compared to control group. There were no statistically significant differences in plasma concentrations of CD among patients with psoriasis and control group. In erythrocytes of patients concentration of CD was twice as high. There were no statistically significant differences in the activity of SOD between the patient group and the control group. Conclusions: The most important product of lipid peroxidation responsible for the formation of psoriatic lesions is MDA. Lack of changes in the concentration of the primary products of lipid peroxidation, with significant changes in the concentration of secondary products of the process in blood of studied subjects can provide a significant degree of severity of damage to the cells.

scholarly journals Gender Features of Radical Oxidation of Lipids in Menopausal Women and Men in Andropause

2016 ◽  
Vol 71 (3) ◽  
Author(s):  
L. I. Kolesnikova ◽  
I. M. Madaeva ◽  
N. V. Semenova ◽  
E. V. Osipova ◽  
M. A. Darenskaya
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Postmenopausal Women ◽  
Control Group ◽  
Double Bonds ◽  
Sod Activity ◽  
Radical Oxidation ◽  
Menopausal Women ◽  
Study Results ◽  
And Control

Aims: Our aim was to assess lipid peroxidation ― antioxidant protection in menopausal women and men in andropause and to compare these processes in different gender and age groups. Materials and methods:74 women and 37 men were examined. This study was a prospective, randomized cohort study. Women were divided into perimenopausal group (n=22, mean age 49.03±3.13), postmenopausal group (n=15, mean age 54.43±4.54) and control (n=37, mean age 34±1.2). Men were divided into a group of andropause (n=20, mean age 50.38±2.63) and control (n=17, mean age 35.21±4.75). Body mass index in the main and control groups was comparable. Questionnaires, clinical examination, assessment of the lipid peroxidation-antioxidant defense system, and the calculation of oxidative stress ratio were conducted to all participants of the study. Results: In women from the reproductive phase transition to its extinction increases content of compounds with conjugated double bonds by 22% (p0.05) in perimenopause and by 27% (p0.05) in postmenopause, increases content of the ketodienes and coupled trienes by 21% (p0.05) in perimenopause relative to the control group and reduced by 27% (p0.05) in postmenopausal women relative to the group of perimenopause. The antioxidant system in women observed the following changes: decrease in the α-tocopherol levels in postmenopausal women by 37% relative to control and by 22% (p0.05) to compare perimenopause; reduction of retinol level by 29% (p0.05) in the perimenopause and by 39% (p0.05) in postmenopause relative to control, increasing of the content of GSSG by 18% (p0.05) in postmenopause to compare control. When comparing groups of men statistically significant differences were not found. When comparing the groups according to gender, we revealed in men the increased content of compounds with conjugated double bonds by 38% (p0.05), the GSSG by 13% (p0.05), reduced content of the ketodienes and coupled trienes by 43% (p0,05), α-tocopherol by 24% (p0.05), SOD activity by 9% (p0.05).Coefficient oxidative stress in perimenopausal women was 2,5, in postmenopausal ― 3,48, in andropause ― 0,97.Conclusions: Expressed lipid peroxidation activity is more physiological in andropause than in menopause. Men in andropause have large functional reserves and adaptive capacity than menopausal women.

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