scholarly journals A comparative study of the virulence factors of Klebsiella spp. Isolated from different environments: دراسة مقارنة لعوامل الضراوة الخاصة ببكتيريا الكليبسيلا Klebsiella spp. المعزولة من بيئات مختلفة

2016 ◽  
Vol 2 (8) ◽  
Author(s):  
Laith Mosleh Naguib , Nagham Mued Hamdy
Keyword(s):  
Comparative Study ◽  
Virulence Factors ◽  
Environmental Samples ◽  
Bacterial Infections ◽  
Bacterial Culture ◽  
Antibiotic Sensitivity ◽  
Bacterial Isolates ◽  
Agar Media ◽  
Different Sources ◽  
Klebsiella Spp

The Current study conducted to investigate of different virulence factors for bacterial isolates resistant to antibiotics and isolated from different environments. where it was collected 247 clinical and environmental samples from different sources isolated on Nutrient agar media.    The results of bacterial culture have shown that 46 isolation dating back to the bacterium Klebsiella spp , isolates  also showed mixed results towards  antibiotics . and antibiotic sensitivity results were selected 18 bacterial isolates resistant to antibiotics to study some of the virulence factors ant to investigate the genes responsible for the kinship between the isolates using (PCR), and the results showed the overall rate of similarity of isolates sick and environmental by 64%, which shows that source of bacterial infections maybe caused by contact with water or soil.

scholarly journals Etiologyand occurrence of acute bacterial meningitis in children in Benghazi Libyan Arab Jamahiriya

1998 ◽  
Vol 4 (1) ◽  
pp. 50-57
Author(s):  
Bandaru Narasinga Rao ◽  
Ibrahim Mahdi Kashbur ◽  
Nuri Mohamed Shembesh ◽  
Suliman Mohamed El Bargathy
Keyword(s):  
Bacterial Meningitis ◽  
Case Fatality ◽  
Antibiotic Sensitivity ◽  
Acute Bacterial Meningitis ◽  
Bacterial Isolates ◽  
Presumptive Diagnosis ◽  
Female Ratio ◽  
Meningitis In Children ◽  
Male To Female ◽  
Klebsiella Spp

Over a 14-month period, 77 children with a presumptive diagnosis of acute bacterial meningitis were investigated. The incidence of acute bacterial meningitis was 0.8%, with a case fatality rate of 13.0%. Children pound 1 year of age were more affected [64.9%]. The total male to female ratio was 1.2:1. Gram stain detected more cases [85.7%] than culture [66.2%]. A total of 48 isolates were identified by culture and their antibiotic sensitivity was determined. Haemophilus influenzae [33.8%] was the predominant organism identified, followed by Streptococcus pneumoniae [26.0%], Klebsiella spp. [6.5%] and Neisseria meningitidis [2.6%]. Many of the bacterial isolates were sensitive to gentamicin, cefotaxime and ceftriaxone and least sensitive to tetracycline and ampicillin

scholarly journals Determination of some Virulence Factors of Pasteurella multocia isolated from Human and Farm Animals infections

2011 ◽  
Vol 5 (3) ◽  
pp. 15-23
Author(s):  
Safaa Abdul-Hadi ◽  
Ismail K. Shobar ◽  
Sawsan S. AL-jubori
Keyword(s):  
Virulence Factors ◽  
Pasteurella Multocida ◽  
Human Lymphocytes ◽  
Antibiotic Sensitivity ◽  
Positive Control ◽  
Farm Animals ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Poultry Tissues

Twenty one Isolates of Pasteurella multocida were obtained from different clinical specimens of human and farm animals' infections. Human specimens included wounds swabs taken after cats and dog's bits beside the urine and sputum samples. The animal samples were nasal swabs and blood samples taken from chattels, also the poultry tissues of infected chicken with fowl cholera were collected. Bacterial isolates were isolated using Pasteurella multocida selective agar (PMSA) then identified doing different morphological, biochemical tests followed by api 20E diagnosis. The ability of the bacterial isolates to produce different virulence factors were studied, 18 isolates 85.7% were able to produce Lipase enzyme. Results of pathogenicity study on Lab. animals (mice) showed that there were 9 highly virulent isolates among the 21 (42.8%). The killing time was in between (10-24) hrs after injected the mice’s intraperitoneally. Some of the isolates showed their ability to produce the dermonecrotic toxin and the positive result appeared as highly purulent, dermonecrotic lesions after injection Genia pig intradermally. Results of antibiotic sensitivity test revealed that there were considerable variations in isolates susceptibility. Some of the isolates were highly resisted most of the used antibiotics while others were not. The crude bacterial extract from PMA20 isolate was tested to determine its ability to stimulate human lymphocytes division in vitro, results showed that the extract was able to stimulate cells division when the mitotic index was 1.2% as compared with the positive control.

scholarly journals BACTERIOLOGICAL STUDY OF EAR DISCHARGE IN BIR HOSPITAL

2003 ◽  
Vol 41 (142) ◽  
pp. 318-322 ◽  
Author(s):  
Charu Arjyal ◽  
S Adhikari ◽  
J Shrestha
Keyword(s):  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Disc Diffusion Method ◽  
Gram Positive ◽  
Bacteriological Study ◽  
Sensitivity Pattern ◽  
Culture Sensitivity ◽  
Acinetobacter Spp ◽  
Klebsiella Spp

Discharge from the ear is one of the commonest symptoms of infections of the ear.With a view to study the major strains of bacteria encountered in ear discharges, thisstudy was conducted in Microbiology Laboratory, Bir Hospital. The study wasconducted for a period of 6 months from January 2000 - July 2000. A total of 224samples of aural discharges of the outpatients were collected and analysed for bacterialgrowth. These samples were cultured with the use of standard microbiologicaltechniques and the antibiotic sensitivity pattern of the isolates was also studied invitro with the use of disc diffusion method. Among 224 patients, 50.9% were male and49.1% were female and the age of the patients varied from 6 months to 76 years.Majority of the patients with discharging ear belonged to the age group 11-20 years.Bacterial growth was found in 82.6%. The bacterial isolates were of 13 differentspecies. Staphylococcus aureus was the most frequently isolated organism (49.4%)followed by Pseudomonas aeruginosa(20.9%). Other organisms isolated wereCoagulase negative Staphylococci (CONS), Streptococcus spp., Bacillus sps., Escherichiaspp., Proteus spp., Enterobacter spp., Klebsiella spp., Acinetobacter spp., Edwardsiellaspp.The prevalence of gram positive bacterial isolates was higher than gram negativebacterial isolates. 58.0% of samples showed positive correlation with direct smeargram stain and culture results. Among the antibiotics used, Ciprofloxacin was foundto be the most effective (80.2%) for overall bacterial isolates followed by Gentamicin(68.9%). The result of antibiotic sensitivity was almost similar to gram positive aswell as gram negative isolates.Key Words: Aural discharge, Gram stain, culture, sensitivity.

scholarly journals Central line-related bloodstream infections and microbiological study in an Egyptian Ministry of Health Hospital

2020 ◽  
Vol 20 (1) ◽  
pp. 158-167
Author(s):  
Khalil Shaaban Nahla ◽  
Ismaeel Sayed Manal ◽  
Mohamed Abdnaby Gehan
Keyword(s):  
Central Venous Catheter ◽  
Bacterial Infections ◽  
Blood Stream Infection ◽  
Antibiotic Sensitivity ◽  
Bloodstream Infections ◽  
Venous Catheter ◽  
Central Line ◽  
Bacterial Isolates ◽  
Central Venous ◽  
Staphylococcus Epidermis

Background: Central line-associated bloodstream infections continue to be one of the most important health problems in ICU. Objectives: To identify the central line-related bloodstream infections and their microbiological characteristics Specific objectives to the identify the incidence, predisposing factors of central line-related bloodstream Infections and study the antibiotic sensitivity patterns of the isolated organisms. Material and Methods: Patients’ demographic, clinical data, central venous catheter related data,and blood culture specimen collection data were collected utilizing a descriptive exploratory design from a purposive sample of 120 ICU patients under- went insertion central venous catheter. Results: out of 120 central line catheters sent for culture from patients immediately and seven days after insertion., 13( 10.83%) were positive seven days after insertion. Of the 13 infection cases, 10 cases were Staphylococcus epidermis and the others were Staphylococcus aureus. In addition, most infected patients were overweight and obese, diabetic, had sub-clavian route, and their length of hospital stayed more than7 days. Conclusion: 10.83 % developed bacterial blood stream infection 7 days after insertion of central venous catheters, and most of bacterial isolates were Staphylococcus epidermis and were sensitive to Vancomycin and Amikain. Keywords: Bacterial infections; bacterial isolates; antibiotic resistance.

Bacteriology and antibiotic sensitivity pattern of ear discharge in patients with chronic otitis media; A cross sectional study conducted in tertiary care hospital of Peshawar.

2021 ◽  
Vol 28 (05) ◽  
pp. 671-676
Author(s):  
Mubassir Ullah ◽  
Muhammad Saleh Faisal ◽  
Ali Zaki ◽  
Azam Khan ◽  
Mamoor Khan ◽  
...  
Keyword(s):  
Otitis Media ◽  
Proteus Mirabilis ◽  
Chronic Otitis Media ◽  
Antibiotic Sensitivity ◽  
Cross Sectional Study ◽  
Bacterial Isolates ◽  
Sectional Study ◽  
Cross Sectional ◽  
Sensitivity Pattern ◽  
Klebsiella Spp

Objective: To determine the frequency of common bacteria and antibiotic sensitivity pattern of ear discharge in patients with chronic otitis media. Study Design: Descriptive Cross Sectional study. Setting: Department of ENT, Northwest General Hospital & Research Centre, Peshawar. Period: February to September 2019. Material & Methods: A total of 196 patients of both gender and age between 13 to 60 years with clinical diagnosis of active chronic otitis media were recruited through consecutive sampling technique. After detailed history and relevant examination, demographic data was recorded and pus specimens were collected from the infected ear on cotton swabs. They were sent to pathology laboratory of hospital for culture of common bacteria (E.coli, Pseudomonas aueroginosa, Proteus mirabilis, Staphylococcus aureus and Klebsiella spp) and their respective antibiotic sensitivity (Co-trimoxazole, Co-Amoxiclav, Ciprofloxacin, Ampicillin, Imipenem, Ceftriaxone and Cefixime). Results: Bacterial isolates were analyzed as 48% Staph aureus, 28% Pseudomonas, 12% Proteus mirabilis, 8% E. coli and 4% Klebsiella spp. Imipenem was sensitive to 94%, ceftriaxone 84%, ciprofloxacin 80%, co-trimoxazole 66%, ampicillin 63%, cefixime 45% and co-amoxiclav against 43% of total bacterial isolates. Conclusion: The common causative organism for active chronic otitis media was Staph aureus followed by Pseudomonas. Imipenem was the most sensitive antibiotic against majority of bacterial isolates followed by ceftriaxone and ciprofloxacin.

scholarly journals The Role of Plasmids in Pathogenicity of Locally Isolated Plesiomonas shigelloides

2011 ◽  
Vol 5 (2) ◽  
pp. 46-54
Author(s):  
M. H. Al-Gelawi ◽  
M. R. Al-Jeboory
Keyword(s):  
Virulence Factors ◽  
Plasmid Dna ◽  
Environmental Samples ◽  
Plasmid Profile ◽  
Bacterial Isolates ◽  
Small Plasmid ◽  
Plesiomonas Shigelloides ◽  
Fish Samples ◽  
Resistance Trait

one hundred and twenty diarrhea samples were collected from different hospitals in Baghdad, four isolates 3.3% (P1, P2, P3, P4) were obtained and identified as Plesiomonas shigelloides from these samples. Four isolates were also obtained and identified as the same bacteria from the environmental samples, two isolates 10% from twenty water samples (termed W1, W2) and two isolates 10% from twenty fish samples (termed F1, F2). Investigation of antibiotic susceptibility was done for all isolates. Some of the virulence factors were determined in this bacterium, like its ability to produce some enzymes. Ability of bacterial isolates to adhesion on epithelial cells was studied as one of important virulence factors; it was found that all isolates have the ability to adhesion on living and non-living surfaces. The plasmid profile for all isolates was studied, and the results showed that the plasmid profile was similar for all isolates. All isolates contained two small plasmid DNA bands approximately in the same sizes. The role of plasmids in pathogenicity of P. shigelloides P1 were studied, throughout cure this isolate using SDS. These results indicated that the trait of β-lactamas, haemolysin and proteinase production in addition to resistance trait for tetracycline and streptomycin were plasmids born. While the lipase and lecithinase production trait were chromosomal born in Plesiomonas shigelloides P1.

scholarly journals Prevalence, Distribution and Antimicrobial Susceptibility Pattern of Bacterial Isolates from a Tertiary Hospital in Malawi

2020 ◽  
Author(s):  
Master Chisale ◽  
Pizga Kumwenda ◽  
Emmanuel C. Adukwu ◽  
Ebot S. Tabe ◽  
Victor C Ujor ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Bacterial Infections ◽  
Antibiotic Sensitivity ◽  
Tertiary Hospital ◽  
Sub Saharan Africa ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Management Options ◽  
Subsequent Increase ◽  
Antimicrobial Susceptibility Pattern

Abstract BackgroundBacterial infections are a significant cause of sickness and death in sub-Saharan Africa. This study aimed at establishing the prevalence, distribution and antimicrobial susceptibility pattern of major bacterial isolates from patients attending a tertiary hospital in Malawi. MethodsWe retrospectively reviewed bacteria culture and antibiotic sensitivity records for 4,617 patients from 2002 to 2014 at Mzuzu Central Hospital (MCH). No inclusion and exclusion criteria were followed. Data was analysed using excel and SPSS programs. ResultsThe most prevalent isolates were S. aureus (34.7%, n= 783), Klebsiella species (17.4%, n= 393) and Proteus species (11.4%, n = 256). Most pathogens were isolated from adults (88.3%, n=3889) and pus was the main source (69.3%, n = 1224). S. pneumoniae was predominantly isolated from cerebrospinal fluid (60.3%, n = 44) largely collected from children (88.2%, n =64). Overall, most bacteria exhibited high resistant to all regularly used antibiotics excluding ciprofloxacin. ConclusionsOur report demonstrates an increase in bacteria burden in sites other than the blood stream and subsequent increase in prevalence of antimicrobial resistance for all major isolates. Creating an epidemiological survey unit at MCH will be essential to help inform better treatment and management options for patients with bacterial infections.

scholarly journals Characterization of Bacteria and Inducible Phages in an Intensive Care Unit

2019 ◽  
Vol 8 (9) ◽  
pp. 1433 ◽  
Author(s):  
Pacífico ◽  
Hilbert ◽  
Sofka ◽  
Dinhopl ◽  
Pap ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intensive Care ◽  
Environmental Samples ◽  
Multidrug Resistant ◽  
Antimicrobial Treatment ◽  
Bacterial Isolates ◽  
Surface Samples ◽  
Klebsiella Spp ◽  
Synergistic Relationship

Intensive care units (ICUs) are critical locations for the transmission of pathogenic and opportunistic microorganisms. Bacteria may develop a synergistic relationship with bacteriophages and more effectively resist various stresses, enabling them to persist despite disinfection and antimicrobial treatment. We collected 77 environmental samples from the surroundings of 12 patients with infection/colonizations by Escherichia coli, Staphylococcus aureus or Klebsiella spp in an ICU in Austria. Surface swabs were tested for lytic phages and bacterial isolates for mitomycin C-inducible prophages. No lytic bacteriophages were detected, but S. aureus was isolated from the surroundings of all patients. About 85% of the colonies isolated from surface samples were resistant to antimicrobials, with 94% of them multidrug resistant. Two inducible temperate bacteriophages—myovirus vB_EcoM_P5 and siphovirus vB_SauS_P9—were recovered from two clinical isolates. Staphylococci phage vB_SauS_P9 lysed S. aureus isolates from the surface swabs collected from the surroundings of three patients. No transductants were obtained on propagation in phage-sensitive antimicrobial-resistant isolates. The two phages were sensitive to 0.25% (v/v) of the disinfectant TPH Protect, which eliminated viable phages after 15 min. Coliphage vB_EcoM_P5 was inactivated at 70 °C and staphylococci phage vB_SauS_P9 at 60 °C after 60 min.

scholarly journals Comparison the Antibacterial Activity of Vitamin D2 and D3

2017 ◽  
Vol 27 (4) ◽  
Author(s):  
Intesar N. Khelkal
Keyword(s):  
Vitamin D ◽  
Antibacterial Activity ◽  
Antibiotic Sensitivity ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Plating Method ◽  
High Turbidity ◽  
Inhibition Zones ◽  
Different Sources

An attempt has been made to determine the antimicrobial activity of vitamin D; D3 & D2 against clinical bacterial isolates as well as perform a comparative analytical study between the effects of both forms of vitamin. The ability of vitamin D (both D2 ergocalciferol& D3 cholecalciferol )  to  inhibit bacterial growth of some clinical isolates have been tested . Forty - three pathogenic bacterial isolates (Gr+ ,Gr- ) have been identified  from fifty - five specimen  was collected from  different sources ; 24 urine, 17 sputum , 9 blood , 5 skin at Al-Kindey hospital for a period of two months . Antibiotic sensitivity was carried out towards 12 different antibiotic discs. The most resistant isolates have been chosen to be tested in the study. Two bacterial suspensions of the selected isolates have been prepared; the first was adjusted to McFarland standard No. 0.5 (1 ×10 8 CFU /mL); the second = 1×1010 CFU /mL. Three concentrations of both vitamins have been prepared; 50,000, 70,000 and 90,000 IU/mL as well as the control ( solvent only ). Antibacterial activity has been examined by using agar diffusion (pore plating method) to determine the most effective concentration among the three concentrations of the two forms of vitamin D. Results were suggesting the important role of vitamin D specially D3 as antibacterial agent .The third concentration (90,000) IU/mL was causing the largest inhibition zone with all tested isolates even with the high turbidity culture (1010CFU/mL) ,followed by the second one (70,000) IU/mL, the lower inhibitor concentration was (50,000) IU/mL. Significant differences have been appeared among the measurements of the diameters of inhibition zones towards three vitamin concentrations when compared one to another and to control.

scholarly journals Isolation and Identification of bacterial isolates from ear infection and their sensitivity to usual antibiotics in human and dogs

2011 ◽  
Vol 35 (1) ◽  
pp. 159-166
Author(s):  
AL- Nassry B. S.
Keyword(s):  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Ear Infection ◽  
E Coli ◽  
Antibiotics Sensitivity ◽  
Zoonotic Bacteria ◽  
Klebsiella Spp

Bacterial examination was done on 50 dogs suffering from ear infection. Dogs examined include both sexes and their ages were between 5 months to 5 years, In the other hand 35 ear smears from male and female human in ages between 7-40 years were also taken . Isolation and identification of bacterial isolates were done to determine species of zoonotic bacteria in human and dogs, also study of antibiotics sensitivity to these isolates. We identified 137 bacterial isolates from dogs ear belonge to 9 bacterial spp. which are : S. aureus( 27.7%) , Klebsiella spp. ( 13.8%) , E. coli ( 13.1%) , Streptococcus spp. ( 12.4%) ,P. haemolytica ( 8.2%) , P. vulgaris ( 7.2%) , S. epidermidis ( 6.5%) , Salmonella spp. ( 5.8%) , P. mirabilis ( 5.1%) . In human ear smears 95 isolates were identified to 11 bacterial spp. as follows : S. aureus( 26.3%) , Streptococcus spp. ( 15.7%) , S. epidermidis ( 13.6%) , Citrobacter spp. ( 9.4%) , P. mirabilis ( 8.4%) , Klebsiella spp. ( 6.3%) , P. vulgaris ( 5.2%) , Salmonella spp. ( 5.2%) , P. aeruginosa ( 4.2%) , Shigella spp. ( 3.1%) , E. coli ( 2.1%) . Antibiotic sensitivity to dog isolates show high sensitivity to Imipenem (IPM) then Streptomycin ( S) then Rifampin ( RA) then Clindamycin ( DA) and lowest to Erythromycin ( E) respectively . but most isolates were resistant to Cefotaxime( CTX) then Ampicillin ( AM) then Vancomycin ( VA) and lowest to Trimethoprim ( TMP) respectively . The human isolates show high sensitivity to Imipenem ( IPM ) then Streptomycin ( S ) then Erythromycin ( E ) then Rifampin ( RA) then Clindamycin ( DA) finally Vancomycin ( VA) . but most isolates were resistant to Cefotaxime ( CTX ) then Ampicillin ( AM) finally Trimethoprim (TMP). P. aeruginosa was resistant to all antibiotics except Imipenem ( IPM) , Streptomycin ( S) and Erythromycin ( E)

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