scholarly journals Evaluation of some Biochemical and Hematological parameters in Pulmonary Tuberculosis patient in Al-Qadisiyah Province

2019 ◽  
Vol 10 (4) ◽  
pp. 3775-3781
Author(s):  
Ali Abdul-Ameer Al-anbaki
Keyword(s):  
Total Protein ◽  
Solid Phase ◽  
Hematological Parameters ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Cross Sectional ◽  
Hematological Analysis ◽  
Wbc Count ◽  
The Impact ◽  
Tuberculosis Disease

The experiment was designed to investigate the impact of tuberculosis in patients with the disease. This cross-sectional study was performed in Al-Qadisiyah province, Iraq. This examination was conducted with 60 males that age ranges from 25-55 years, which divided into 30 males with Tuberculosis disease and 30 voluntaries healthy as a control group. Assay of study evaluation of parameters levels that include: Bodyweight Albumin, total protein, AST, ALT,  urea and creatinine, ESR, Hb, and WBC count. Also, the study included Zn estimation by using solid-phase enzyme-linked immunosorbent assay.  The results reported a significant (P<0.05) decreased in biochemical investigation in this study (Bodyweight, albumin, total protein,  and  ALT)  while  the  hematological  analysis  showed substantial decreased  in  Hb  but  exhibited  a  significant (P<0.05) increases in ESR and WBC count in male's patients with Tuberculosis disease as compared with healthy groups. Although, the result showed significant (P<0.05) decreased in Zn level in a patient with Tuberculosis compared with control, While AST doesn't show significant (P<0.05) compared with control. Urea and Creatinine levels were significantly decreased in patients Tuberculosis disease compared with control healthy.  The current study concluded that tuberculosis disease affected the liver and kidney by decreasing the protein and enzyme produced in the liver, and the kidney also causes a defect in hematological parameters in patients with the disease. 

scholarly journals The impact of smoke from grilled fish on the hematological parameters of Indonesian grilled fish sellers

2019 ◽  
Vol 34 (2) ◽  
pp. 160-167
Author(s):  
Dwi Purbayanti ◽  
Rinny Ardina ◽  
Syahrida Dian Ardhany ◽  
Rudi Gunawan ◽  
Mohammad Rizki Fadhil Pratama
Keyword(s):  
Hematological Parameters ◽  
Test Group ◽  
Mean Value ◽  
Control Group ◽  
Long Term Effects ◽  
Mixed Cell ◽  
Content Type ◽  
Cell Parameters ◽  
Hematological Analysis ◽  
The Impact

Purpose Fish processing by grilling can produce emissions that contain toxic compounds that can have short- and long-term effects on human health. Another study reported that exposure to air pollutants is hematotoxic. The purpose of this paper is to determine the effect of smoke exposure on fish grill results on hematological parameters. Design/methodology/approach The subjects of this study were 90 grilled fish sellers, with 32 processed food sellers who did not sell grill food as a control. The hematological analysis was performed using the Hematology Analyzers KX300 instrument. Findings The results showed that the mean value of hematological parameters in the test group was higher than the control group except for the number of lymphocytes and mixed cell parameters. Originality/value The content of harmful compounds contained in fish grill smoke can increase hematological value in the blood of exposed individuals, which has the potential for health problems and disease progression.

The effect of ethanol on some hematological parameters of the australian red claw crayfish (Cherax quadricarinatus)

2022 ◽  
pp. 85-91
Author(s):  
D. Skafar ◽  
D. Shumeyko
Keyword(s):  
Body Weight ◽  
Total Protein ◽  
Hematological Parameters ◽  
Group Versus ◽  
Control Group ◽  
Total Protein Content ◽  
Cherax Quadricarinatus ◽  
Total Hemocyte Count ◽  
Red Claw Crayfish ◽  
Experimental Group

Purpose: to study the effect of ethanol on the parameters of THC, the percentage of granulocytes and total protein in the hemolymph of the Red claw crayfish (Cherax quadricarinatus).Materials and methods. The object of this experiment was 26 males of the Australian red-clawed crayfish (Cherax quadricarinatus) weighing from 23 to 83 g. The individuals were evenly divided into two experimental groups - with an injection of ethanol and a control group without an injection of 13 crayfish for each group. The injection dose was 2515 mg per 100 g of body weight. A day after the introduction of ethanol, hemolymph was taken with a syringe from the ventral sinus, the syringe was pre-washed with a 4% EDTA-Na2 solution. Three parameters were determined: the total hemocyte count (THC), percent granulocytes and percent total protein content. Counting of hemocytes and determination of granulocytes were performed in a Goryaev chamber under a light microscope. The total protein was determined by the refractometric method.Results. Differences in THC and total protein between the groups were statistically unreliable (p>0,05). THC in the experimental group is 36% more than in the control group. The total protein after the introduction of ethanol actually increased by 0,7%, and relatively by 14%. There were statistically different indicators of the proportion of granulocytes (p<0,05) - the average value of 33,1% in the experimental group versus 24,5% in the control group. A reliable (p<0,05) strong feedback was revealed between the total protein and the mass of individuals in both experimental groups, while in the experimental group there is a visible shift in the values of dependent hemolymph indicators towards an increase in smaller individuals.Conclusion. A single injection of ethyl alcohol with a dosage of 2515 mg per 100 g of body weight into the hemolymph of C. quadricarinatus does not cause significant changes in the THC and total protein after 24 hours. At the same time, the proportion of granulocytes actually increases by 9%, relative to 37%. This may indicate that granulocytes are involved in the formation of cancer defense mechanisms when exposed to toxic substances. The effect of different dosages of ethanol injections and the duration of its effect on hematological parameters requires additional consideration. It is necessary to investigate its effect on other indicators, such as the pH and buffer capacity of the hemolymph, the concentration of hemocyanin, glucose, lactates and calcium.

scholarly journals Subchronic toxicity of aqueous extract of Alstonia boonei de wild. (apocynaceae) stem bark in normal rats

2015 ◽  
Vol 3 (1) ◽  
pp. 5 ◽  
Author(s):  
Barnabé Lucien Nkono Ya Nkono ◽  
Selestin Dongmo Sokeng ◽  
Paul Désiré Dzeufiet Djomeni ◽  
Frida Longo ◽  
Pierre Kamtchouing
Keyword(s):  
Aqueous Extract ◽  
Biochemical Study ◽  
Female Rats ◽  
Male Rats ◽  
Control Group ◽  
Control Male ◽  
Control Female ◽  
Hematological Analysis ◽  
Wbc Count ◽  
Dose Dependent

<p><strong>Methodology:</strong> Wistar rats were randomly assigned into eight groups of five animals each: four male groups and four female groups. Each sex group had a control group receiving distilled water and three test groups receiving 200, 500 and 1000mg/kg respectively. Animal’s body weights were recorded on the first day and once a week for the four experiment weeks. The hematological analysis included total WBC count, total RBC count, Hb, %HCT, MCV, MCH and MCHC. Biochemical/serum profile studies include TG, TC, ALT, AST, urea and TP. Tissue specimens of the liver, kidney and lung were subjected to histological examination using standard hematoxylin-eosin staining.</p><p><strong>Results:</strong> In male rats, aqueous extract showed significant decreases in relative weight of liver with extreme significance P&lt;0.001 at a dose of 200mg/kg (vs. control group), P&lt;0.001 of lung at all the doses, P&lt;0.05 (200 and 500mg/kg) and P&lt;0.01 (1000mg/kg) in heart weight. In relative kidney weight, only the dose of 1000mg/kg showed a significant increase vs. normal control male rats. Unlike male rats, only relative kidney weight in female rats was significantly different from the control group in a dose-dependent manner. The aqueous extract treated male groups showed significant increases P&lt;0.001 (1000mg/kg) of total WBC count and MCHC, significant decreases of %HTC (dose response manner), P&lt;0.05 total RBC count (at doses of 500 and 1000mg/kg) and Hb P&lt;0.01 (500mg/kg) vs. normal male rats. In female rats, the haematological study showed significant increase P&lt;0.01 of total WBC count (at the doses of 500 and 1000mg/kg), significant decreases P&lt;0.05 and P&lt;0.01 of total RBC respectively at the doses of 200 and 1000mg/kg, significant decrease of Hb with extreme significance P&lt;0.001 at the dose 1000mg/kg, %HTC also decrease dose response manner vs. control female rats. Biochemical study showed in male rats significant decreases in level of TG P&lt;0.001 (at the doses of 200 and 500mg/kg) and urea, although it showed any dose-dependent effect vs. control male rats. AST also decreases (P&lt;0.05) in male rats at the dose of 200mg/kg but significantly increase P&lt;0.001 at the dose of 500mg/kg. In the female rats, biochemical study revealed significant increases in level of TG P&lt;0.001 and urea P&lt;0.01 at the dose of 200mg/kg and significant decreases in level of TG P&lt;0.01, AST P&lt;0.05 and urea P&lt;0.05 at the dose of 500mg/kg (vs. control female rats). Microscopically, there were mild hepatic and renal tissue injuries supporting the hematological analysis.</p><p><strong>Conclusion:</strong> The results indicated that aqueous extract of <em>Alstonia boonei</em> De Wild is toxic in high doses.</p>

scholarly journals Analysis of Endocan Levels in Hypertensive Patients as Risk Factors of Chronic Kidney Disease

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Suryani Jamal ◽  
Uleng Bahrun ◽  
Ibrahim Abdul Samad ◽  
Fitriani Mangarengi ◽  
Hasyim Kasim ◽  
...  
Keyword(s):  
Renal Disease ◽  
End Stage Renal Disease ◽  
Stage Ii ◽  
University Hospital ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Elisa Method ◽  
Cross Sectional ◽  
Stage Renal Disease ◽  
End Stage

This study aimed to analyze endocan levels as a marker of endothelial dysfunction in the control group, patients withstage I hypertension, stage II hypertension, and patients with end-stage renal disease. Endocan levels were measured withESM-1 (endocan) kit by Enzyme-Linked Immunosorbent Assay (ELISA) method. This study used a cross-sectional methodand was conducted in Dr. Wahidin Sudirohusodo Hospital, Makassar and Hasanuddin University Hospital from Septemberto October 2017. There were 83 samples in this study, consisting of 12 samples in the control group, 22 samples of stage Ihypertension, 28 samples of stage II hypertension, and 21 samples of end-stage renal disease aged 20-90 years old. Thisstudy showed significantly higher endocan levels in patients with stage II hypertension and end-stage renal disease(p< 0.05). Endocan levels were significantly higher (p<0.05) in patients with end-stage renal disease compared with thecontrol group and patients with stage I hypertension; but not significantly higher (p > 0.05) compared to patients with stageII hypertension. Also, the median of endocan levels in patients with the end-stage renal disease was higher (309,850 ng/L)compared to patients with stage II hypertension (273,050 ng/L).

scholarly journals Profile of Estrogen Metabolism in Endometriosis Patients

2016 ◽  
Author(s):  
Tirsa Verani ◽  
 Kanadi Sumapradja
Keyword(s):  
Blood Level ◽  
Obstet Gynecol ◽  
Statistical Significance ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Estrogen Metabolism ◽  
Control Group ◽  
Cross Sectional ◽  
Keywords Endometriosis ◽  
Test Result

Objective: To assess the estrone (E1), estradiol (E2) and estriol (E3) blood level and its ratio (E2:E1, E2:E3 and E1:E3) between women with and without endometriosis. Method: We performed an analytical cross sectional study with 27 women with endometriosis and 27 women without endometriosis who met the inclusion criteria. The samples were recruited in Dr. Cipto Mangunkusumo hospital and other satellite hospitals from October 2012 to April 2013. The blood level of estrogen metabolites was examined by enzyme-linked immunosorbent assay (ELISA). Comparison between the two groups was analyzed by using Mann- Whitney test. Result: The level of estrone was found to be lower in endometriosis group compared to that in the control group (54.66 pg/ml vs 73.52 pg/ml, p=0.229). Similarly, the levels of estradiol and estriol were lower in endometriosis group (29 pg/ml vs 35 pg/ml, p=0.815 and 1.11 pg/ml vs 1.67 pg/ml, p=0.095, consecutively). The E2:E1 ratio was higher in endometriosis group (0.51 pg/ml vs 0.38 pg/ml, p=0.164), as well as E2:E3 ratio (26.53 pg/ml vs 21.11 pg/ml, p=0.223) and the E1:E3 ratio (58.55 pg/ml vs 50.28 pg/ml, p=0.684). However, all those differences were not statistically significant. Conclusion: The estrone, estradiol and estriol levels in women with endometriosis were lower compared to those in women without endometriosis. The ratio of E2:E1, E2:E3 and E1:E3 were higher in endometriosis group. However, all those differences failed to reach statistical significance. [Indones J Obstet Gynecol 2014; 3: 127-131] Keywords: endometriosis, estradiol, estriol, estrogen, estrone

scholarly journals THE IMPACT OF ASTHMAAND ITS MEDICATIONS ON DENTAL CARIES STATUS AND STREPTOCOCCUS MUTANS COUNT IN CHILDREN: A CROSS-SECTIONAL STUDY

2020 ◽  
pp. 20-23
Author(s):  
Sneha Upadhyay ◽  
Jyoti Bhavthankar ◽  
Mandakini Mandale ◽  
Nivedita Kaorey
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Cross Sectional Study ◽  
Control Group ◽  
Healthy Children ◽  
Sectional Study ◽  
Cross Sectional ◽  
Asthmatic Children ◽  
Significant Difference ◽  
The Impact

Background: Asthma and its medications have been linked to oral diseases in asthmatic children. Aim: Assessment of the dental caries status, salivary Streptococcus mutans count and S. mutans colony score in children receiving inhaled anti asthmatic medications and their comparison in healthy children Material and Method: A cross-sectional study was performed on 40 asthmatic children and 40 healthy children in the age group of 6-14 years. DMFT/deft indices were calculated and saliva samples were collected. Diluted saliva was inoculated on MSB agar plates. S. mutans count and colony score were analysed after 24-48 hours of inoculation. Results: Statistically significant difference was observed in the mean DMFT/deft index, salivary S. mutans load and S. mutans colony score in children of the asthmatic group and control group. Conclusion: Prevalence of dental caries and cariogenic bacteria is higher in asthmatic children.

scholarly journals Impact of the Neuregulin rs35753505 C/T Polymorphisms on Neuregulin 1 Levels in Preterm Infants

2019 ◽  
Vol 7 (12) ◽  
pp. 1931-1934
Author(s):  
Bugis Mardina Lubis ◽  
Sjarif Hidajat Effendi ◽  
Ratna Akbari Ganie ◽  
Oke Rina Ramayani
Keyword(s):  
Preterm Infants ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
P Value ◽  
Nucleotide Polymorphisms ◽  
Gene Encoding ◽  
Cross Sectional ◽  
Neuregulin 1 ◽  
Organ Systems ◽  
The Impact

BACKGROUND: Neuregulin (NRG) 1 plays an important role in the development of various organ systems in human. Single nucleotide polymorphisms rs35753505 C/Tof the gene encoding NRG1 evident as allele C and T with genotypes of CT, CC, and TT are believed to have an impact on NRG1 levels.AIM: To determine the impact of the NRGrs35753505 C/T polymorphisms on NRG1 levels in preterm infants.METHODS: A cross-sectional study was conducted from February to December 2018, whereas 48 eligible preterm infants with a gestational age of 32- < 37 weeks were enrolled. An umbilical cord blood specimen was collected for determination of NRG1 levels with enzyme-linked immunosorbent assay (ELISA) and NRG1 polymorphisms with polymerase chain reaction (PCR). Statistical analysis was performed with 95%CI and P value of < 0.05 was considered statistically significant.RESULTS: Median value of NRG1 levels (174.4 pg/ml) served as a cut off value. NRG 1 polymorphisms composed distribution of CC (31%), CT (42%), TT (27%) genotypes and distribution of C and T alleles were 52% and 48%. The median NRG1 levels in CC and CT genotypes were significantly lower compared to TT genotype (151.1 pg/ml vs 407.2 pg/ml, P = 0.005 and 159.1 pg/ml vs 407.2 pg/ml, P = 0.009). Subjects with C allele had significantly lower median NRG1 levels than T allele (151.1 pg/ml vs 407.2 pg/ml, P = 0.002). Subjects with CC and CT genotypes had higher risk to develop lower NRG1 levels compared to TT genotype (OR = 8.25, P = 0.016 and OR = 10.74, P = 0.005, respectively).CONCLUSION: Allele C is associated with lower NRG1 levels. Preterm infants with CC and CT genotypes pose a higher risk to have lower NRG1 levels.

scholarly journals Acute and sublethal intoxication of malathion in an Indian major carp, Labeo rohita: haematological and biochemical responses.

2021 ◽  
Vol 36 (3) ◽  
pp. e2021016
Author(s):  
Juginu Mankuzhiyil Sivanandan ◽  
Binukumari
Keyword(s):  
Sublethal Effects ◽  
Labeo Rohita ◽  
Hematological Parameters ◽  
Organophosphorus Pesticide ◽  
Control Group ◽  
Indian Major Carp ◽  
Biochemical Responses ◽  
Lc50 Value ◽  
Hematological And Biochemical Parameters ◽  
The Impact

This study aimed to determine the median lethal concentration (96-h LC50), acute and sublethal effects of malathion, an organophosphorus pesticide on hematological and biochemical responses in an Indian major carp, Labeo rohita. In this study, the LC50 value of malathion for 96 h was found to be 3.4 ppm. During acute (3.4 ppm) and sublethal [1/10th of 96 h LC50 value (0.34 ppm) studies, all the hematological parameters except WBC were significantly decreased (p<0.05). Besides, when compared with the control group, a significant (p<0.05) decrease in biochemical activity was also observed in malathion treated fish during acute and sublethal exposure periods. These results suggest that the tested concentrations of malathion could have significant adverse effects on the hematological and biochemical parameters of fish, Labeo rohita. The changes in the parameters can be effectively used to determine the impact of malathion in the aquatic ecosystem.

scholarly journals Effect of β-glucan on behavioral, biochemical and hematological parameters against toxicity of copper sulfate in common carp Cyprinus carpio L.

2014 ◽  
Vol 38 (2) ◽  
pp. 128-137
Author(s):  
Jamal K. Al-faragi,
Keyword(s):  
Common Carp ◽  
Copper Sulfate ◽  
Cyprinus Carpio ◽  
Hematological Parameters ◽  
Control Group ◽  
Enzymes Activity ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Wbc Count ◽  
Hb Concentration

The aim of the present study was to assess the effect of a commercial ß-glucan against acute toxic effect of copper sulfate on common carp Cyprinus carpio L. behavioral, hematological parameters and biochemical tests. A total of 250 Fish (150 ± 2 g) were fed a basal control diet or the basal diet plus a ß-glucan (1 g kg-1 diet) for 60 days. LC50 was calculated for 72 h which was 10.83 mg L-1.  At the end of the trial, CuSO4.5H2O was added to all treatments  at a dose  of  5 mgL-1  for  T1  and  T2, 7mg L-1  for  T3  and  T4, 9  mgL-1 for T5  and  T6   for  96h. Fish behavioral changes were recorded during 96h of exposure to different Cu concentrations with and without β-glucan, which showed abnormalities such as increase swimming activity, jerky movement, loss of equilibrium, respiratory stress, increase operculum movement, frequent jumping, erratic swimming and swimming near the water surface. On the other hand, ß-glucan supplemented groups showed significant increase (P≤0.05) in Hb concentration, PCV%, RBC and WBC count in comparison with groups without ß-glucan. The results of dietary groups without ß-glucan showed a significant decrease (P≤ 0.05) in Hb concentration, hematocrit value, RBC count, but there were significant increase (P≤ 0.05) in WBC count with an increase of Cu concentration compared to control groups. Results showed significant increase (P≤ 0.05) in dietary groups without ß-glucan in values of ALT, AST and ALP enzymes activity with elevation of Cu levels compared to control group. But, ß-glucan supplemented groups showed significant decrease (P ≤ 0.05) in values of ALT, AST and ALP enzymes activity in comparison with dietary groups without ß-glucan. These results suggested that β-glucan has protective effect against CuSO4 toxicity and future studies should be pursued particularly immune response and molecular studies

scholarly journals Relationship between CBC and ESR and duration of disease in patients with systemic lupus erythematosus (SLE)

2018 ◽  
Vol 13 (2) ◽  
pp. 41-46
Author(s):  
Mahmuda Abira ◽  
Qazi Shamima Akhter
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Complete Blood Count ◽  
Hematological Parameters ◽  
Significant Negative Correlation ◽  
Control Group ◽  
Systemic Lupus ◽  
Initial Manifestation ◽  
Cross Sectional ◽  
Duration Of Disease

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease and is associated with considerable morbidity. Hematological abnormalities may be the initial manifestation. Duration may have impact on their hematological parameters. Objective: To observe the relationship of Complete Blood Count (CBC) and Erythrocyte sedimentation Rate(ESR) with duration of disease in patients with SLE. Methods: This cross sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2016 to June 2017. Total 60 subjects including both sexes were selected with age ranging from 18 to 55 years. Among them, 30 subjects with SLE were study group and 30 age matched healthy subjects were control group. Total count of RBC, WBC, Platelet and ESR were estimated by automated hematology analyzer. Statistical analysis was done by unpaired Students æt” test, Chi Square test and Pearson’s correlation coefficient (r) test. Results: In this study, ESR was significantly (p<0.001) higher and RBC,WBC and platelet counts were significantly (p<0.001) lower in SLE patients in comparison to that of control. On correlation analysis, RBC and WBC showed significant negative correlation with duration of disease in patients with SLE. Conclusion: This study concludes that CBC and ESR were altered in SLE patients and it was related to duration of disease. J Bangladesh Soc Physiol. 2018, December; 13(2): 41-46

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