scholarly journals Perbedaan metode pembuatan VCO antibakteria terhadap sifat Fisikokimia, dan uji organoleptik, Antibakteri VCO terhadap Bakteri Staphylococcus Aureus

Jurnal Gizi ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 124
Author(s):  
Octo Zulkarnain ◽  
Suci Ferdiana
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Lauric Acid ◽  
Chronic Wounds ◽  
Bacterial Cells ◽  
Test Results ◽  
Test Analysis ◽  
Gram Positive Bacteria ◽  
Bacterial Content ◽  
Barrier Zone

Wounds have slowed healing due to the formation of biofilms, which are the surface formation of bacterial cells that are covered by the polymeric substance. Staphylococcus aureus is a gram-positive bacteria that play a role in producingmultilayer in biofilms and is very easy to resistant to antibiotics. VCO has the ability as ana anti-bacterial content based on lauric acid and kaprat acid. This study used experimental Quasy with a mixed Methode method. VCO is made with 3 methods, fishing, heating and mixing on physicochemical test. Physicochemical test results show, the highest water content is found in the VCO heating, the same three-type weight, the highest peroxide number is in the VCO mixing and the highest free fatty acids on the VCO fishing. The results of antibacterial test with Kirby-Baur method There is no barrier zone from the 3 types of  VCO. VCO used as a topical is VCO fishing.The results of a test analysis of the results of observations in patients as much as 15 people for 9 days in 3 treatments with chronic wounds obtained p < 0.05 (0.000) which means during the treatment that the wound condition is better. Keyword : Organoleptic, Staphylococcus aureus, Physicochemical, VCO

scholarly journals Wall Teichoic Acid Protects Staphylococcus aureus against Antimicrobial Fatty Acids from Human Skin

2009 ◽  
Vol 191 (13) ◽  
pp. 4482-4484 ◽  
Author(s):  
Thomas Kohler ◽  
Christopher Weidenmaier ◽  
Andreas Peschel
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Fatty Acid ◽  
Teichoic Acid ◽  
Sebaceous Glands ◽  
Wall Teichoic Acid ◽  
Fatty Acid Binding ◽  
Gram Positive Bacteria ◽  
Skin Colonization ◽  
Wall Teichoic Acids

ABSTRACT Skin-colonizing gram-positive bacteria produce wall teichoic acids (WTAs) or related glycopolymers for unclear reasons. Using a WTA-deficient Staphylococcus aureus mutant, we demonstrated that WTA confers resistance to antimicrobial fatty acids from human sebaceous glands by preventing fatty acid binding. Thus, WTA is probably important for bacterial skin colonization.

scholarly journals Unsaturated Fatty Acids Control Biofilm Formation of Staphylococcus aureus and Other Gram-Positive Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 788
Author(s):  
Kamila Tomoko Yuyama ◽  
Manfred Rohde ◽  
Gabriella Molinari ◽  
Marc Stadler ◽  
Wolf-Rainer Abraham
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Linoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Gram Positive ◽  
Biofilm Inhibition ◽  
Gram Positive Bacteria ◽  
Long Chain ◽  
Chain Fatty Acids

Infections involving biofilms are difficult to treat due to increased resistances against antibiotics and the immune system. Hence, there is an urgent demand for novel drugs against biofilm infections. During our search for novel biofilm inhibitors from fungi, we isolated linoleic acid from the ascomycete Hypoxylon fragiforme which showed biofilm inhibition of several bacteria at sub-MIC concentrations. Many fatty acids possess antimicrobial activities, but their minimum inhibitory concentrations (MIC) are high and reports on biofilm interferences are scarce. We demonstrated that not only linoleic acid but several unsaturated long-chain fatty acids inhibited biofilms at sub-MIC concentrations. The antibiofilm activity exerted by long-chain fatty acids was mainly against Gram-positive bacteria, especially against Staphylococcus aureus. Micrographs of treated S. aureus biofilms revealed a reduction in the extracellular polymeric substances, pointing to a possible mode of action of fatty acids on S. aureus biofilms. The fatty acids had a strong species specificity. Poly-unsaturated fatty acids had higher activities than saturated ones, but no obvious rule could be found for the optimal length and desaturation for maximal activity. As free fatty acids are non-toxic and ubiquitous in food, they may offer a novel tool, especially in combination with antibiotics, for the control of biofilm infections.

scholarly journals Single Clove Garlic (Allium sativum) Essential Oil as an Inhibitor of Staphylococcus aureus Bacteria

2019 ◽  
Vol 11 (1) ◽  
pp. 77-83
Author(s):  
Abdul Gofur ◽  
Ida Wulandari ◽  
M. Fitri Athoillah ◽  
Agung Witjoro ◽  
Sri Rahayu Lestari
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Morphological Structure ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Bacterial Cells ◽  
Test Analysis ◽  
Garlic Essential Oil

Staphylococcus aureus is gram-positive bacteria that often infect the skin. S. aureus has been experiencing resistance to several antibiotics. One of the solutions to overcome the resistance is by using garlic that is believed by the society can overcome bacterial infection. The study aimed to find out the influence of the variation in concentration of local single clove garlic essential oil used on the inhibition and damage of morphological structure of S. aureus bacteria. The inhibition test was conducted using disc diffusion method. The experiment groups consisted of  1% DMSO as negative control, vancomycin 30µg/ml as positive control and single clove garlic essential oil (25 mg/ml, 50 mg/ml, 75 mg/ml and100 mg/ml) as treatment groups. The diameter of inhibition zone was measured using calipers. The morphological damage of the bacterial cells can be seen using Scanning Electron Microscopy (SEM) with magnification of 25000x. The result of Kruskal-Wallis test analysis indicated that the extract of local garlic essential oil has inhibitory activities against S. aureus bacteria (P<0.05). The damage to the morphological structure of bacterial cells with the administration of 30 µg/ml vancomycin was equal to 100 mg/ml single clove garlic essential oil extract. Single clove garlic essential oil can be used as an alternative treatment for skin infection diseases by inhibiting S. aureus growth.

scholarly journals UJI AKTIVITAS ANTIBAKTERI KRIM ASAM LAURAT TERHADAP Staphylococcus aureus ATCC 25923 DAN Pseudomonas aeruginosa ATCC 27853

2016 ◽  
Vol 2 (2) ◽  
pp. 59-67
Author(s):  
Evi Sulastri ◽  
Mappiratu Mappiratu ◽  
Annisa Kartika Sari
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Acid Concentration ◽  
Lauric Acid ◽  
Storage Time ◽  
Test Results ◽  
Concentration Variance ◽  
The Stability ◽  
Dispersive Power

Lauric acid is a saturated fatty acid found in fatty/vegetable oils, especially in palm oil. Lauric acid was reported to posses antiviral and antibacterial property. This study aims to determine the effect of the concentration variance of lauric acid in cream on the stability of the cream physical quality and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. In this research, lauric acid concentration in cream was varied in 10%, 20%, and 30% and then tested for antibacterial activity using hole plate technique. The results of the evaluation of lauric acid cream preparation of Formula 1, Formula 2 and Formula 3 showed that the organoleptic and homogeneity tests of all formula was no difference during storage time, but the result of pH, viscosity, and dispersive power of all formula has difference during storage time (28 days). The antibacterial test results showed that the highest inhibition diameter was found in the use of lauric acid concentration of 30% for S.aureus (21.589 mm ± 0.18) and P.aeruginosa (14.25 ± 0.37 mm). Based on statistical analysis of the cream produced from the three concentrations of lauric acid, they were not stable because there are significant differences in pH, viscosity, and dispersive power during storage time (28 days). Keywords: lauric acid, antibacterial cream, Staphylococcus aureus, Pseudomonas aeruginosa.

scholarly journals The antibacterial activity of Tembelekan leaf (Lantana camara L.) and Kopasanda leaf (Chromolaena odorata L.) extracts against Staphylococcus aureus

2020 ◽  
Author(s):  
Sri Anggarini Rasyid ◽  
Sugireng ◽  
Ridwan Adi Surya ◽  
Sanatang ◽  
Rosdarni ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Lantana Camara ◽  
Test Results ◽  
Chromolaena Odorata ◽  
Leaf Extracts ◽  
Average Value ◽  
Gram Positive Bacteria ◽  
Significant Difference

Staphylococcus aureus (S. aureus) is a round-shaped Gram-positive bacteria that caused infection in the human body. S. aureus infection can be inhibited by the use of antibiotics. However, these bacteria displayed resistance to some antibiotics. The purpose of this study is to determine the effects of Tembelekan (Lantana camara L.) and Kopasanda (Chromolaena odorata L.) leaf extracts against S. aureus. The method used in this study is the disc method to determine the inhibition zone. The test results showed there was no significant difference in the sensitivity of Tembelekan leaf extract (L. camara L.) and Kopasanda leaf extract (C. odorata L.) against S. aureus (P0.8>α0.05). However, based on the average value of the inhibition zone, Kopasanda leaf extract was showed higher inhibition zone compared to Tembelekan leaf extract. In conclusion, Tembelekan leaf and Kopasanda leaf at a concentration of 100% were produced inhibition zones of 0.6 cm and 0.8 cm respectively against S. aureus.

scholarly journals Unraveling the Molecular Mechanism of Selective Antimicrobial Activity of 2(5H)-Furanone Derivative against Staphylococcus aureus

2019 ◽  
Vol 20 (3) ◽  
pp. 694 ◽  
Author(s):  
Irshad Sharafutdinov ◽  
Anna Pavlova ◽  
Farida Akhatova ◽  
Alsu Khabibrakhmanova ◽  
Elvira Rozhina ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Molecular Mechanism ◽  
Rapid Development ◽  
Direct Interaction ◽  
Bacterial Cells ◽  
Cell Integrity ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Life Threatening

Staphylococcus aureus causes various infectious diseases, from skin impetigo to life-threatening bacteremia and sepsis, thus appearing an important target for antimicrobial therapeutics. In turn, the rapid development of antibiotic resistance and biofilm formation makes it extremely robust against treatment. Here, we unravel the molecular mechanism of the antimicrobial activity of the recently unveiled F105 consisting of three pharmacophores: chlorinated 2(5H)-furanone, sulfone, and l-menthol moieties. F105 demonstrates highly selective activity against Gram-positive bacteria and biofilm-embedded S. aureus and exhibits low risk of resistance development. We show explicitly that the fluorescent analogue of F105 rapidly penetrates into Gram-positive bacteria independently of their cell integrity and viability and accumulates there. By contrast, Gram-negative bacteria remain impermeable and, therefore, insusceptible to F105. Apparently, in bacterial cells, F105 induces reactive oxygen species (ROS) formation and nonspecifically interacts with a number of proteins, including ROS-utilizing ones. Using native and 2D PAGE, we confirm that F105 changes the charge of some proteins by either oxidation or direct interaction with them. Therefore, it seems justified to conclude that being simultaneously a ROS inducer and damaging proteins responsible for ROS utilization, F105 impairs the cellular anti-ROS defense representing a prospective ROS-inducing antibacterial agent.

scholarly journals Packed Like Sardines – How Surface Crowdedness Impacts Accessibility to Peptidoglycan of Staphylococcus aureus

2020 ◽  
Author(s):  
Noel J. Ferraro ◽  
Marcos M. Pires
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Cell ◽  
Dense Layer ◽  
Bacterial Cells ◽  
Gram Positive ◽  
Teichoic Acids ◽  
Gram Positive Bacteria ◽  
Wide Range ◽  
Small Molecule Therapeutics ◽  
Live Bacterial Cells

AbstractBacterial cell walls are essential barriers that protect bacteria against the onslaught of potentially lethal molecules from the outside. Small molecule therapeutics, proteins from bacterial foes, and host immune proteins must navigate past a dense layer of bacterial biomacromolecules (e.g., capsular proteins, teichoic acids, and anchored proteins) to reach the peptidoglycan (PG) layer of Gram-positive bacteria. A subclass of molecules (e.g., antibiotics with intracellular targets) must also permeate through the PG (in a molecular sieving manner) to reach the cytoplasmic membrane. In the case of Staphylococcus aureus (S. aureus), teichoic acids are the major biopolymers that decorate bacterial cell surfaces. Despite the biological and therapeutic importance of surface accessibility, systematic analyses in live bacterial cells have been lacking. We describe a novel live cell fluorescence assay that reports on the permeability of molecules to and within the PG scaffold. The assay has robust reproducibility, is readily adoptable to any Gram-positive organism, and is compatible with high-throughput sample processing. Analysis of the factors controlling permeability to S. aureus and the methicillin resistant MRSA revealed that molecular flexibility plays a central role in molecular permeability. Moreover, teichoic acids impeded permeability of molecules of a wide range of sizes and chemical composition.

scholarly journals Volatiles and Lipoidal Composition: Antimicrobial Activity of Flowering Aerial Parts of Lavandula pubescens Decne

2017 ◽  
Vol 9 (08) ◽  
Author(s):  
Gouda B. ◽  
Mousa O. ◽  
Salama M. ◽  
Kassem H.
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Unsaponifiable Matter ◽  
Volatile Oil ◽  
Gram Positive ◽  
Gram Negative ◽  
Butanol Fraction ◽  
Gram Positive Bacteria ◽  
Aerial Parts

The hydro-distilled volatile oil of the flowering aerial parts of Lavandula pubescens Decne. was analyzed using gas chromatography-mass spectrometry (GC/MS). Twenty eight components were identified representing 87.39% of the total oil. Carvacrol (22.39 %), cis-β-Farnesene (13.25 %) and β-Bisabolene (12.9 %) were the major constituents. Lipoids were detected in the n-hexane extract. Unsaponifiable lipoids (USL) and fatty acids methyl esters (FAME) of the n-hexane extract were analyzed by GC/MS. The percentage of the total identified unsaponifiable matter was 83.51%, while that of fatty acids was 40.83%. 5-Hydroxy1,3,4-trimethoxy-7-methyl-6-propar-naphthalene was the major identified component in the unsaponifiable matter representing 36.64 %, followed by Hentriacontane (8.09 %). Octadecenoic acid was the major fatty acid identified representing 12.72 %.The antimicrobial potential of the methanol extract and its fractions (n-hexane, methylene chloride, ethyl acetate and n-butanol) as well as the hydrodistilled volatile oil were assessed. All the tested samples except the n-butanol fraction exhibited broad spectrum activity against the tested Gram-positive bacteria;Bacillus subtilis, Staphylococcus aureus and Streptococcus faecalisas well as Gram-negative bacteria; Escherichia coli, Pseudomonas aeruginosa and Neisseria gonorrhea. The n-butanol fraction showed antimicrobial activity against all tested Gram-negative and Gram-positive bacteria except Staphylococcus aureus. The growth of Candida albicans and Aspergillus flavus was not affected by any of the tested samples.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Experience of using atraumatic ointment bandages based on peruvian balsam in the complex treatment of patients with chronic wound defects of the lower limbs

2020 ◽  
pp. 43-57
Author(s):  
Anna Tabuika
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Chronic Wounds ◽  
Chronic Wound ◽  
Local Treatment ◽  
Lower Limbs ◽  
Complex Treatment ◽  
Average Area ◽  
Arterial Insufficiency ◽  
Significant Activation

The article reflects the results of a retrospective non-comparative study, the objects of which were 34 outpatient comorbid patients (15 of which are over 60 years old) with chronic wounds of the lower limbs developed against the background of varicosity, post-thrombotic disease, chronic arterial insufficiency of the lower limbs, diabetes mellitus or their combination. Their local treatment was carried out using atraumatic ointment dressing «Branolind N» containing Peruvian balsam. There were 23 women (67.6 %) and 11 men (32.4 %). In microbiological study prior to the beginning of treatment in 31 patients the growth of a pathogen of wound infection was revealed; in 19 patients — Staphylococcus aureus in monoculture and in various associations, in 6 patients — Pseudomonas aeruginosa in monoculture, in other cases — other pathogens. In 3 patients the pathogen was not detected. The average wound size was 34 cm2 . The phase of the wound process was additionally confirmed by cytological studies. After treatment the average area of the wound defect decreased by 10 cm2 and made 24 cm2 on average. Full healing of the wound defect occurred in 11 patients, the others had granulation and active marginal epithelization. There was also a decrease in bacterial semination of wounds, a change in composition of infection agents to less aggressive monoflora, and cytologically — a decrease in signs of inflammation against the background of significant activation of reparative processes.

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