Aktivitas antihiperurisemia teh asam daun tin (Ficus carica) terhadap tikus putih (Rattus norvegicus)

<div class="WordSection1">AbstrakTin (Ficus carica) merupakan tanaman yang memiliki aktivitas antioksidan sehingga dapat digunakan sebagai alternatif pengobatan penyakit degeneratif salah satunya yaitu hiperurisemia. Aktivitas antioksidan tersebut dapat ditingkatkan melalui proses fermentasi. Hasil dari proses fermentasi tersebut berupa teh asam. Tujuan penelitian ini adalah untuk mengetahui aktivitas antihiperurisemia teh asam daun tin pada tikus putih (Rattus norvegicus). Pengujian dilakukan terhadap 5 kelompok kontrol negatif (Pakan tinggi purin 10,8 ml/kgBB), kontrol positif (Allopurinol 9 ml/kgBB), teh asam daun tin dosis 20, 25 dan 30 ml/kgBB. Pemberian sediaan uji setiap hari selama 8 hari. Pengukuran kadar asam urat dengan menggunakan metode fotometrik enzimatis. Pengambilan sampel darah melalui vena jantung pada hari ke 22. Hasil pengukuran kadar asam urat dengan pemberian teh asam daun tin dengan dosis 30 ml/kgBB menunjukkan kadar asam urat paling rendah dibandingkan dengan kontrol positif. Dapat disimpulkan bahwa teh asam daun tin dengan dosis 30 mL/kgBB memiliki aktivitas antihiperurisemia terhadap hewan uji. Kata Kunci: antihiperurisemia, fermentasi kombucha, teh asam daun tin.Antihyperuricemic activity of fig (Ficus carica) sour tea on male rat (Rattus norvegicus) AbstractFig (Ficus carica) are known to have antioxidant activity, thus it can be used as an alternative treatment of degenerative diseases, such as hyperuricemia. The antioxidant activity of Fig can be increased by the fermentation process. The result of the fermentation process is sour tea. This study aimed to investigate the antihyperuricemic activity of Fig (Ficus carica) sour tea on male rat (Rattus norvegicus). This research was a True experiment study with the design of Post Test Control Group. Research samples were divided into 5 groups, in which there were 3 rat in each group. Group 1 was the positive control (Allopurinol 1,8 ml/200grBW), and Group II was the negative control (High purin diet 2,16 ml/grBW). In addition, the Group III, IV, and V were the experimental group with different dose of sour tea of Fig; 4ml/200grBW/day, 5ml/200grBW/day, and 6ml/200grBW/day for 8 days. Identification of uric acid levels was performed using enzymatic photometric method. Meanwhile, the serum samples were taken from the heart vein on the 22nd day of experiment. The results the best dose of sour tea of Fig in lowering the level of uric acid in male rat (Rattus norvegicus) was 6 ml/200grBW/day (3,87 mg/dl) compared to the positive controls which had the average level of 4,2 mg/dl. In addition, the result of One Way ANOVA demonstrated the significant difference in the probability value of 0,05. Therefore, it can be concluded that sour tea of Fig (Ficus carica) can effectively lower the uric acid level of male (Rattus norvegicus). Keywords: antihyperuricemic, kombucha fermentation, sour tea of Fig leaves.</div>

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Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

Jurnal Profesi Medika Jurnal Kedokteran dan Kesehatan ◽

10.33533/jpm.v14i1.1591 ◽

2020 ◽

Vol 14 (1) ◽

Author(s):

Ni Made Ridla Parwata

Keyword(s):

Body Weight ◽

Physical Exercise ◽

Rattus Norvegicus ◽

Research Method ◽

Male Rat ◽

Control Group ◽

Adult Male Rat ◽

The Subject ◽

Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

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ASSOCIATION BETWEEN SERUM URIC ACID AND METABOLIC SYNDROME

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i10.27233 ◽

2018 ◽

Vol 11 (10) ◽

pp. 400 ◽

Cited By ~ 1

Author(s):

Thaslima Nandhini Js ◽

Savitha Basker G ◽

Vishnupriya V

Keyword(s):

Metabolic Syndrome ◽

Uric Acid ◽

Venous Blood ◽

Fasting Blood Glucose ◽

Control Group ◽

Serum Triglycerides ◽

Significant Difference ◽

Disease Condition ◽

Student’S T ◽

Student’S T Test

Objective: Metabolic syndrome is a cluster of disease condition characterized by truncal obesity, hypertriglyceridemia, elevated blood pressure, and insulin resistance. An excessive circulating uric acid (UA) level even within normal range is always comorbid with metabolic syndrome and its components. The aim of the current study was to investigate the association between metabolic syndrome and serum UA level.Methods: A total of 60 subjects were divided into two groups of healthy (30 individuals) and metabolic syndrome patients (30 individuals) from dental outpatient department of Saveetha Dental College and Hospitals. 5 ml of fasting venous blood was collected in the plain collection tubes and centrifuged, and then serum was separated. Then, the serum was used to analyze the fasting blood glucose, serum triglycerides (TGLs), and serum UA by GOD-POD, enzymatic colorimetric, and uricase method, respectively. A statistical analysis was performed using Student’s t-test. p<0.05 was considered to be statistically significant.Result: Mean body mass index (BMI), fasting blood sugar (FBS), TGL, and UA level of control group were 23.36±1.81, 84.45±13.1, 110.9±22.6, and 3.48±1.21 respectively. Mean BMI, FBS, TGL, and UA level of study group were 35.24±3.04, 122.85±23.3, 212.1±39.6 and 9.08±2.63 respectively. There is a significant difference between these two groups with p<0.0001.Conclusion: This study showed that those individuals with metabolic syndrome have higher UA level that indicates hyperuricemia which is a significant predictor of metabolic syndrome.

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PENGARUH GELATIN IKAN PATIN (PANGASIUS DJAMBAL) TERHADAP JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI TIKUS PUTIH (RATTUS NORVEGICUS)

ODONTO Dental Journal ◽

10.30659/odj.6.1.1-5 ◽

2019 ◽

Vol 6 (1) ◽

pp. 1

Author(s):

Fredy Mardiyantoro ◽

Fidya Fidya ◽

Dena Savira Andriani

Keyword(s):

Rattus Norvegicus ◽

Tooth Extraction ◽

Control Group ◽

Fish Gelatin ◽

Tukey Test ◽

White Rats ◽

Significant Difference ◽

Post Hoc ◽

Hematoxylin Eosin ◽

Test Result

Background: Gelatin has been used as a hemostatic agent to stop bleeding and it can be absorbed by human body within 6 hours. The patin fish (Pangasius djambal) gelatin has a high amount of amino acid, especially glutamine which has an important role in increasing the proliferation of fibroblast in wounds. The purpose of this study was to determine the effect of patin fish gelatin to the number of fibroblast in wound after tooth extraction.Method: Rat’s tooth extraction is done on the lower left incisive tooth. Thirty white rats were divided into two groups randomly with 3 time series. Control group was the rats without given of patin fish gelatin and treatment group was the rats given 1cc of patin fish gelatin with pipette. Socket taken for histologically processed by Hematoxylin Eosin staining.Result: It showed there were significant differences in the number of fibroblasts between groups. The Post Hoc Tukey test result showed there was a significant difference between the control group and the treatment group.Conclusion: Patin fish gelatin has effect increasing the number of fibroblast inwound after white rat’s (Rattus norvegicus) tooth extraction

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The Relationship between Serum Levels of Vitamin C, Uric Acid and Antioxidant Condition with Coronary Artery Diseases: a Case-Control Study

10.21203/rs.2.15439/v1 ◽

2019 ◽

Author(s):

Aida Torkzaban ◽

Seyed Amir Mansour Alavi Naeini ◽

Akbar Hassanzadeh ◽

Mehrdad Namdari

Keyword(s):

Uric Acid ◽

Vitamin C ◽

Case Control Study ◽

Demographic Data ◽

Systolic Pressure ◽

Case Control ◽

Control Group ◽

Case Group ◽

Significant Difference ◽

Control Study

Abstract Background Coronary hearth diseases are among the main causes of death in adults. Increase of oxidative stress and defects in antioxidant defense play a major role in endothelium performance and are an effective factor in progress of atherosclerosis. Some studies have also reported different malondialdehyde and total antioxidant capacity among the atherosclerosis patients.Methods In this case-control study, 44 atherosclerosis patients referring to Shahid Madani treatment-education center were considered as the case group; while 44 healthy peoples were placed in the control group. Demographic data and anthropometric indices were measured. Food frequency questionnaire and international physical activity questionnaires were also completed. After 12 hours of fasting, 10 ml blood was sampled from the participants. Uric acid, vitamin C, TAC and MDA were also measured. The data were finally analyzed by SPSS Ver 22 software.Results A significant difference was observed between the two groups in terms of uric acid (P<0.001) and vitamin C (P<0.03). However, mean MDA and TAC showed no significant difference between the two groups. The two groups’ difference in terms of vitamin A, E and beta carotene, zinc and selenium intake was not significant. A significant difference was however detected between the two groups in terms of vitamin C (P<0.047). A significant relationship was also observed between the systolic pressure and CHD (P<0.028).Conclusion Results of this study indicated that the uric acid and vitamin C levels of atherosclerosis patients had significant increase and decrease in comparison with the healthy subjects, respectively. Mean TAC and antioxidant levels of their diets (except for vitamin C) showed no significant difference. Systolic blood pressure of the patients was significantly higher than the controls.

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Examining Metabolic Profiles in Opioid-‎Dependent Patients ‎

International Journal of Medical Toxicology and Forensic Medicine ◽

10.32598/ijmtfm.v10i3.28681 ◽

2020 ◽

Vol 10 (3) ◽

pp. 28681

Author(s):

Nader Molavi ◽

Amir Ghaderi ◽

Hamid Reza Banafshe

Keyword(s):

Control Group ◽

Study Group ◽

Metabolic Profiles ◽

Opioid Use ◽

Serum Samples ◽

Social Burden ◽

Study Results ◽

Significant Difference ◽

Hs Crp ◽

Opioid Users

Background: Drug abuse is a social burden and a public health disorder. Previous evidence suggested numerous illicit substances (e.g., opioids, amphetamines, cocaine, & cannabis) affect immune system functions, oxidative stress mechanisms, inflammatory cytokines, and reactive oxygen species production. This study aimed to determine the extent of these metabolic parameters in opioid-dependent patients. We also compared these patients with a healthy control group. Methods: This study was conducted in Amirie Clinic, Kashan, Iran. Plasma and serum samples from 50 illicit opioid users (study group) and 50 non-opioid users (control group) were studied. Metabolic levels for MDA, NO, TAC, GSH, Insulin, HOMA-IR, and hs-CRP were assessed in both research groups (N=100). Results: There was a significant difference in the status of MDA (P=0.003), NO (P=0.01), TAC (P=0.003), GSH (P=0.001), insulin (P=0.04), HOMA-IR (P=0.02), and hs-CRP (P=0.001) between the study and control groups. Furthermore, there was a significant correlation among the duration of illicit opioid use and MDA concentrations (r=-0.424, P=0.002), as well as TAC levels (r=0.314, P=0.02). Conclusion: The study results suggested metabolic profiles were impaired in the study group, compared to the controls.

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Can IL-2R Alpha be a Valuable Marker along with Ca 19–9 in the Diagnosis of Chronic Pancreatitis and Pancreatic Cancer?

The International Journal of Biological Markers ◽

10.1177/172460080401900304 ◽

2004 ◽

Vol 19 (3) ◽

pp. 196-202

Author(s):

B. Kayhan ◽

M. Akdoğ;an

Keyword(s):

Pancreatic Cancer ◽

Chronic Pancreatitis ◽

Interleukin 2 ◽

Carbohydrate Antigen ◽

Control Group ◽

Serum Samples ◽

Cancer Group ◽

Abdominal Symptoms ◽

Significant Difference ◽

Inflammatory Processes

Background Pancreatic cancer is characterized initially by non-specific abdominal symptoms followed by rapid tumor progression. Although chronic pancreatitis is a benign disorder, it can be one of the causative factors of pancreatic cancer. The level of the tumor marker carbohydrate antigen 19–9 (CA 19–9) in pancreatic cancer does not correlate with the stage of the neoplasm. Soluble interleukin 2 receptor (sIL-2R) is a cytokine that shows increased levels during some inflammatory processes and malignant disorders. Aim Our aim in this study was to investigate whether sIL-2Rα levels can be used in association with CA 19–9 in the early diagnosis of pancreatic cancer and chronic pancreatitis. Patients Serum samples were obtained from the blood of 21 pancreatic cancer patients without distant metastasis who were deemed inoperable, 16 chronic pancreatitis patients and 20 normal volunteers. Results We did not find any significant differences in CA 19–9 levels between normal controls and patients with chronic pancreatitis. There was a significant difference in the levels between the control group and the pancreatic cancer group (p=0.003) and between patients with chronic pancreatitis and those with pancreatic cancer (p=0.004). Although there was no significant difference in sIL-2Rα levels between the control group and the patient groups, we found a slight correlation between sIL-2Rα and CA 19–9 levels in the pancreatic cancer group (p=0.003, r=0.623) and a more marked correlation in the chronic pancreatitis group (p<0.01, r=0.751). Conclusion According to our results, sIL-2Rα alone is not a good candidate marker in the diagnosis of pancreatic cancer; it can, however, be used in association with CA 19–9 for this purpose.

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Folate-receptor 1 level in periodontal disease: a pilot study

BMC Oral Health ◽

10.1186/s12903-019-0909-z ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 1

Author(s):

Duygu Alkan ◽

Berrak Guven ◽

Cigdem Coskun Turer ◽

Umut Balli ◽

Murat Can

Keyword(s):

Periodontal Disease ◽

Gingival Crevicular Fluid ◽

Folate Receptor ◽

Serum Folate ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Serum Samples ◽

Pocket Depth ◽

Probing Pocket Depth ◽

Significant Difference

Abstract Background The purpose of this study was to investigate gingival crevicular fluid (GCF) and serum folate-receptor 1 (FOLR1) levels in subjects with different periodontal status. Methods The study consists of three groups: Healthy group (n = 15), gingivitis group (n = 15) and chronic periodontitis group (n = 15). Clinical periodontal parameters including probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI) and bleeding on probing (BOP) were assessed. GCF and serum samples were collected from each patient and were analyzed FOLR1 levels by enzyme-linked immunosorbent assay. Results The values of FOLR1 in GCF were higher in gingivitis and periodontitis groups than among patient in control group (p < 0.016). Serum FOLR1 levels showed no significant difference between the groups. A significant correlation was observed between FOLR1 levels of GCF and BOP (p < 0.05). Conclusions Our preliminary data suggest that FOLR1 is not useful in monitoring the periodontal disease. Further studies are necessary to clarify the role, regulation and function of folate and it’s receptors in the pathogenesis of periodontal disease.

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Association of the waist-hip ratio with plasma adiponectin related to coronary artery disease

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v17i2.35886 ◽

2018 ◽

Vol 17 (2) ◽

pp. 290-295

Author(s):

Premtim Rashiti ◽

Ibrahim Behluli ◽

Albiona Bytyçi

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Medical Science ◽

Control Group ◽

Serum Samples ◽

Waist Hip Ratio ◽

Significant Difference ◽

Artery Disease ◽

A Prospective Study ◽

And Control

Objective: By enrolling a prospective study of 82 patients that underwent non-urgent coronary angiography for coronary artery disease (CAD), it is aimed to investigate the correlation between adiponectin and waist-hip-ratio with severity of CAD.Materials and methods: The results of the angiography, divided the patients into two groups, patients admitted with a diagnosis of CAD and non-CAD. In the conducted hospital based research, two groups were involved: the study group with documented angiographically CAD and control group without angiographic evidence of CAD. Some of the baseline adiponectin levels in stored serum samples of all patients, anthropometric and biochemical risk factors were assessed in both groups.Result and discussion: As the result, we have seen the presence of CAD that was associated with current smoking, male gender, waist–hip ratio (WHR).While, no significant difference between median adiponectin levels at baseline were observed between cases and controls.Conclusion: There is a significant positive correlation between waist - hip ratio and presence of severity of coronary artery disease.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.290-295

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Hepatitis C Virus Infection and B-Cell Non-Hodgkin’s Lymphoma.

Blood ◽

10.1182/blood.v106.11.4668.4668 ◽

2005 ◽

Vol 106 (11) ◽

pp. 4668-4668

Author(s):

Janet G. Grudeva

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

B Cell ◽

Hcv Infection ◽

Control Group ◽

Cell Infection ◽

Serum Samples ◽

Pathogenetic Role ◽

Significant Difference

Backgroud: An increasing number of bacterial and viral infections have been linked with specific subtypes of lymphoma. Preliminary evidence suggests that hepatitis C virus (HCV) might play a pathogenetic role in autoimmune-related, non-malignant B-cell lymphoproliferation, as well as a subset of B-cell non-Hodgkin, s lymphomas (B-NHL), often with extranodal localization. Design and methods: The study was conducted in the Department of Hematology and consisted 149 (86 male, 63 female) untreated patients with a new diagnosis of B-NHL for 5-years period (2000–2004). HCV infection was investigated by testing for HCV antibodies in serum samples. The controls were 587 patients (without intravenous drug users) in other departments of the same hospital. Results: HCV infection was documented in 13 cases (8,4%) with NHL. The infected patients were not clinically relevant cryoglobulinemic activity, increased rate of autoimmune disorders and extranodal localizations prevalence. There was statistically significant difference between the NHL and control group (p<0,01) and no statistically significant difference between man/women carriers (p>0,05) into the NHL group. Overall, the clinical outcome of HCV-positive NHL does not seem to be different from that of NHL patients without HCV infection. However, the evidence of a significant liver injury may predict a worse prognosis in these cases. Conclusions: Our date suggest that HCV infection may be associated with B-NHL. With regard to the mechanism(s) by which HCV might favor B-cell expansion and malignant transformation, most date support an indirect pathogenetic role of the virus as an exogenous trigger. A direct oncogenetic role of HCV by direct cell infection and deregulation has only been hypothesized on the basis of the lymphotropism of the virus.

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Cytokine expression profiles of patients with acute myelogenous leukemia (AML) and non-Hodgkin lymphoma (NHL) detected by multiplex assay

Journal of Clinical Oncology ◽

10.1200/jco.2007.25.18_suppl.18530 ◽

2007 ◽

Vol 25 (18_suppl) ◽

pp. 18530-18530

Author(s):

V. B. Reddy ◽

D. K. Oelschlager ◽

J. S. Nolan ◽

K. L. Taylor ◽

J. Post ◽

...

Keyword(s):

Expression Profiles ◽

Recovery Phase ◽

Cytokine Expression ◽

Multiplex Assay ◽

Myelogenous Leukemia ◽

Control Group ◽

Serum Samples ◽

Expression Levels ◽

Significant Difference ◽

Low Levels

18530 Background: To determine the cytokine expression profiles of patients with AML and NHL using a sensitive bead-based Luminex multiplex assay in a routine clinical diagnostic setting. Methods: Blood (plasma/serum) samples were collected from ten AML and five NHL patients. Six control samples from patients diagnosed as non-neoplastic/non-autoimmune/non-inflammatory were also analyzed for comparison. All samples were frozen prior to analysis. Using a bead-based Luminex assay (Human Cytokine 8-Plex Assay, Bio- Rad, Hercules, CA) we analyzed these samples for a panel of cytokines (IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, and TNF-alpha). This assay uses polystyrene microspheres, which provides simultaneous quantitation of these cytokines in a single sample. The expression levels were presented in picograms/mL. Average values for each of these markers were obtained for each group of patients (AML versus NHL versus Controls), and their expression levels were compared using χ2 analysis. Results: Overall, there was a significant difference in the expression profiles of all these cytokines among three patients groups (χ2, P < 0.001). All cytokines were consistently expressed at low levels in NHL patients as compared to control group. However, the levels of IL-6 and IL-8 were increased by 2.7 and 5.8 times, respectively in AML patients as compared to controls. Conclusions: The low levels of cytokines in NHL and AML patients suggest suppressed immune system in these two disease conditions; however, these findings warrant further studies to explore the underlying mechanisms for the increased levels of IL-6 and IL-8 in AML patients. Currently, studies are in progress to compare the levels of cytokines measured by Luminiex assay in different stages of leukemias and lymphomas (initial, post treatment and recovery phase etc.). These studies are partially funded by grants from the National Institute of Health/National Cancer Institute (RO1-CA98932–01 and U24-CA086359). No significant financial relationships to disclose.

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