scholarly journals The effect of noni fruit (Morinda citrifolia L.) extract on interleukin 4 and interleukin 10 in Wistar rats, induced by pyrazinamide, ethambutol and levofloxacin

2019 ◽  
Author(s):  
Thontowi Djauhari Nur Subchi ◽  
Merryana Andriani
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Cell Damage ◽  
Interleukin 10 ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Interleukin 4 ◽  
Test Results ◽  
Fruit Extract

Pyrazinamide, Ethambutol and Levofloxacin are part of the anti-tuberculosis SLD that have many side effects. This response causes an inflammatory reaction that affects pro-inflammatory cytokines interleukin 4 and interleukin 10. Morinda citrifolia L. produces anti-inflammatory activity that affects cytokines and provides protection against cell damage. A dose therapy was administered to wistar rats for two months, grouped in K- (negative control), K+ (positive control), MI (drug + Morinda citrifolia L. extract, dose of 100 mg/kg body weight), MII (drug + noni fruit extract, dose of 200 mg/kg body weight) and MIII (drug + noni fruit extract, dose of 400 mg/kg body weight). Results were examined using Rat IL-4 and IL-10 immunoassay Quantikine ELISA kits. The ANOVA (Analysis of Variance) test results showed that there was an overall IL-4 difference (sig<0.05); for IL-10 there was no great difference (sig>0.05); it was decrease trend was shown in MI and MII. However, by giving Morinda citrifolia L. extracts can significantly influence the IL-4 and IL-10, with a decreasing trend in MI and MII.

Effect of Topical Noni (Morinda citrifolia L.) Leaf Extract Paste in Carrageenan-induced Paw Edema on Wistar Rats

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Indah Puti Rahmayani Sabirin ◽  
Euis Reni Yuslianti
Keyword(s):  
Inflammatory Response ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Morinda Citrifolia ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The inflammatory response is one of natural process in the body to protect itself following tissue injury, but it can cause discomfort. Noni (Morinda citrifolia L.) leaf known as a traditional medicament to help reduce the inflammatory effect. The leaves empirically applied as a wrapping on fever or wound. The purpose of this study was to identify the anti-inflammatory effect of topical noni leaf extract paste in 5% and 10% concentration by examination of Wistar rat paw edema induced by λ-carrageenan. Twenty-four Wistar rats divided into four groups, which were negative control, positive control with diclofenac sodium 1% gel, 5% noni leaf, and 10% noni leaf paste groups. Paw edema was induced by intraplantar injection of 1% λ-carrageenan to every rat. Every treatment subsequently applied in the plantar area before injection, and the changed paw volume measured with plethysmometer at minutes 0, 30, 60, and 90. This study was at the Animal Laboratory, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi city in October–December 2017. The result displayed that the minimum volume after 90 minutes was on 5% and 10% noni leaf paste group, which is 1.00 mL. Kruskal-Wallis test result of inflammatory percentage was significantly different among every group in each examination time (p<0.05). Post-hoc test showed that inflammatory reduction on paw edema with noni leaf paste application on both concentrations were significantly different compared to the negative control. However, it was not different from the positive control group. This study showed that application of noni leaf paste in 5% and 10% concentration could help reduce inflammatory response on skin possibly by the active anti-inflammatory ingredients of noni leaf. EFEK PASTA EKSTRAK DAUN MENGKUDU (MORINDA CITRIFOLIA L.) TOPIKAL TERHADAP EDEMA KAKI TIKUS GALUR WISTAR YANG DIINDUKSI KARAGENANInflamasi adalah proses alami tubuh untuk melindunginya setelah cedera, namun hal tersebut dapat menyebabkan ketidaknyamanan. Daun mengkudu (Morinda citrifolia L.) dikenal sebagai obat tradisional untuk menurunkan efek inflamasi yang secara empiris digunakan untuk mengobati demam dan luka. Tujuan penelitian ini adalah mengetahui efek anti-inflamasi pasta ekstrak daun mengkudu melalui pemeriksaan edema kaki tikus yang diinduksi karagenan-λ. Dua puluh empat tikus galur Wistar dibagi menjadi 4 kelompok, yaitu kontrol negatif dengan akuades, kontrol positif (aplikasi gel Na diklofenak 1%), serta perlakuan pasta daun mengkudu 5% dan 10%. Edema dibuat dengan menginjeksi intraplantar tikus dengan 1% karagenan-λ pada tiap kelompok. Tiap-tiap perlakuan diaplikasikan sebelum tikus diinjeksi dan perubahan volume kaki tikus diukur dengan pletismometer di menit ke-0, 30, 60, dan 90. Penelitian ini dilakukan di Laboratorium Hewan, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Kota Cimahi pada Oktober–Desember 2017. Hasil pengukuran memperlihatkan penurunan volume edema kaki terkecil setelah 90 menit pada kelompok pasta daun mengkudu 10%, yaitu 1,00 mL. Hasil Uji Kruskal-Wallis terhadap persentase inflamasi berbeda nyata pada tiap kelompok dan tiap waktu pengamatan (p<0,05). Hasil uji beda menunjukkan bahwa penurunan inflamasi kaki tikus pada perlakuan pasta daun mengkudu kedua konsentrasi berbeda nyata dibanding dengan kontrol negatif, tetapi tidak berbeda dengan kontrol positif. Penelitian ini menunjukkan bahwa pasta daun mengkudu 5% dan 10% dapat membantu menurunkan reaksi inflamasi kulit dan efeknya sejalan dengan Na diklofenak karena zat aktif yang bersifat anti-inflamasi dalam daun mengkudu.

scholarly journals Antimalaria assay of fruit extract of Morinda citrifolia and activity of mice (Mus musculus) macrophage after infecting it with Plasmodium berghei

2005 ◽  
Vol 3 (2) ◽  
pp. 61-69
Author(s):  
RAHADI HUTOMO ◽  
SUTARNO SUTARNO ◽  
WIEN WINARNO ◽  
KUSMARDI KUSMARDI
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
World Wide ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Distilled Water ◽  
Inhibition Activity ◽  
Fruit Extract ◽  
Macrophage Activity

Malaria is a world wide disease. Death resulting from the disease was caused by the parasite’s resistance to the malaria drugs and the problem of immune system. The aims of the research were to know the effect of M. citrifolia fruit extract to Plasmodium berghei on total red blood cells of mice, and to know the effect of the extract on the number of intraperitoneal macrophage phagocytosing latex after infected by P. berghei. Three doses of fruit extract, 200, 150, 100 mg/kg BB were used in this study. Fansidar was used as positive control, while distilled water was used as negative control. The result of this research indicated that dose of 200 mg/kg BB could reduce number of parasitemia to 3.576%, dose of 150 mg/kg BB to 4.107%, and dose of 100 mg/kg BB to 13.331% on day 5, and could not reduce any number of parasitemia on the next day. Inhibition by Fansidar reached 0.201%, while distilled water did not show any inhibition activity. Different macrophage activity on phagocytosing latex was taken place. The average of macrophage activity on phagocytosing latex at dose of 200 mg/kg BB was 3.8x106 cell, at dose of 150 mg/kg BB was 2.53x106 cell/mL, while at dose 100 mg/kg BB was 1.5x106 cell/mL, and 2.43x106 cell/mL for the control. Based on the results of the study, it can be concluded that the reduction of the number of parasitemia taken place at dose 200 and 150 mg/kg BB, although its activity is much lower than malaria drug of Fansidar. Macrophage activities increased at dose of 200 mg/kg BB.

scholarly journals The Effect of Vernonia amygdalina on Chloroquine-induced Cardiotoxicity in Adult Wistar Rats

2019 ◽  
pp. 1-9
Author(s):  
Rebecca O. Adebayo ◽  
Peterson M. S. Atiba ◽  
Gabriel G. Akunna ◽  
Olaleye O. Olabiyi
Keyword(s):  
Body Weight ◽  
Cardiac Muscle ◽  
Wistar Rats ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Sod Activity ◽  
Male Adult

Background: Cardiotoxicity could result from chemotherapeutic drugs or other medications used in disease control such as antimalarial drugs. Chloroquine (C.Q), an antimalarial agent has also been used in the treatment of rheumatoid arthritis, giardiasis, extra-intestinal amebiasis and lupus erythematous. However, its cardiotoxic roles have been documented. Vernonia amygdalina del. (V.A) has been reported to exhibit antioxidant and cytoprotective activities. These ameliorative and protect effects have been attributed to the presence of flavonoids. There is a paucity of data to support the cardioprotective potentials of this important neutraceutical. We aimed to evaluate the possible effects of Vernonia amygdalina on Chloroquine-induced cardiotoxicity in Wistar models. Materials and Methods: Twenty-four male adult Wistar rats were randomized into four groups of six rats each: I, Control: given normal feed and water ad libitum for 28 days; II, administered 30 mg/kg body weight chloroquine orally for 28 days; III, administered 30 mg/kg body weight of chloroquine orally for 28 days and with 400mg/kg bodyweight of Vernonia amygdalina for another 14 days; VI, administered Vernonia amygdalina 400mg/kg body weight for 28 days. Antioxidant parameters [malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD)], and histology of rat cardiac muscles were examined in the different groups. Results: There was a significantly (p<0.0.05) increase in MDA level, reduced GSH level, increased SOD activity, and altered microanatomy of the rat cardiac muscle in the positive control group when compared with those of the negative control group. The changes in MDA and GSH concentration and SOD activity parameters were significantly (p<0.0.05) mitigated in rats co-treated with V.A when compared with the positive control rats. Similarly, co-administration of V.A with C.Q inhibited chloroquine induced-cardiotoxicity by reducing the altered microanatomy of the cardiac muscle of the rat. Conclusion: It was concluded that V.A ameliorated chloroquine-induced cardiotoxicity in rats via its antioxidant property.

scholarly journals The Use of Torbangun Leaf Powder (Coleus amboinicus L) in Increasing Prolactin in Breastfeedings Wistar Rats and Bodyweight of the Litters

2021 ◽  
Vol 3 (1) ◽  
pp. 26-33
Author(s):  
Stella T Hasianna ◽  
July Ivone ◽  
Sifa D Shinta ◽  
R Zahra Nadhira
Keyword(s):  
Body Weight ◽  
Breast Milk ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Breast Milk Production ◽  
Significant Difference ◽  
Leaf Powder ◽  
Coleus Amboinicus

Research on local herbs that have the effect of increasing breast milk production isneeded to help mothers with insufficient breastmilk. This research was conducted to determinethe effect of torbangun (Coleus amboinicus L) leaf powder in increasing prolactin inbreastfeeding Wistar rats and the bodyweight of the litters. Thirty breastfeeding Wistar rats weredivided into 5 groups with 5 litters each. The treatments given were 108 mg, 216 mg, and 432 mgtorbangun leaf powder (TLP), with domperidone as the positive control, and CMC 10% as thenegative control. The treatment was given 3 times/day for 14 days. Tukey's HSD means testshowed a significant difference in prolactin levels between the negative control (NC), TLP1, andTLP2 groups and the TLP3 and positive control (PC) groups with all of them having p = 0.000.There was no difference in the increase in body weight of litters between treatment groups (p =0.866). In conclusion, torbangun leaves increase the prolactin levels in breastfeeding rats buthave no difference in the increase in body weight of litters between treatment groups. Keywords: torbangun leaves; prolactin; bodyweight; breastfeeding

scholarly journals Uji Anti Jamur Ekstrak Buah Mengkudu (Morinda citrifolia L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 92-102
Author(s):  
Ririen Hardani ◽  
I Kadek Adi Krisna ◽  
Baharuddin Hamzah ◽  
Muhammad Fakhrul Hardani
Keyword(s):  
Candida Albicans ◽  
Measurement Data ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Fruit Extract ◽  
Different Types ◽  
The Media ◽  
Inhibitory Power

Noni is a plant that has the potential to be used as traditional medicinal ingredients. This study aims to determine the inhibition of noni extracts (Morinda citrifolia L.) in inhibiting the growth of the fungus Candida albicans. This type of research is experimental. A total of 150 grams of dried noni fruit that has been mashed, macerated with three different types of solvents namely aquades, acetone and n-hexane to obtain thick extract. Preparation of the fungus suspension was Candida albicans made by mixing the media Nutrient Broth with 5 ose test fungus that were equated with turbidity of a standard MC. Farland. The fungus suspension made was implanted on the Sabouroud Dextrose agar to solidify and divided into 7 sterile petri dishes. Testing the inhibition of fungi using the method of wells. Each cup is made of 1 hole diameter of 3 cm in the center. Tests were carried out by adding non-solvent aquades fruit extract into the 1 cup, acetone noni fruit extract into the cup 2, n-hexane solvent noni fruit extract into the cup 3, for 3 other plates added with three solvents as negative control, while for 1 cup added to the cup fungal infection drug is nystatin as a positive control, each as much as 1 mL. Measurement data were analyzed using the formula of the percentage of inhibition. Inhibitory power of noni fruit extract using distilled water is 3.55%, noni fruit extract using acetone solvent is 50.15%, noni fruit extract using n-hexane solvent that is equal to 38.83%. negative control of aquades, acetone and n-hexane solvent has no inhibitory power while positive control of nystatin is 9.73%. Noni fruit extract using acetone solvent has the strongest inhibitory ability. It can be concluded that the noni fruit extract has fungal inhibitory effect on the growth of the fungus Candida albicans

scholarly journals Antidiabetic Potential Screening of Ulin Fruit Extract (Eusideroxylon zwageri) Against Streptozotocin-Induced Diabetic Rats

2022 ◽  
Vol 1 ◽  
pp. 1
Author(s):  
Muhammad Rizani Faisal ◽  
Nada Widiasmira Amanda Oktia ◽  
Diana Aulia Mursidah ◽  
Dede Mahdiyah ◽  
Bayu Hari Mukti
Keyword(s):  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Test Results ◽  
Fruit Extract ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Average Body ◽  
Eusideroxylon Zwageri ◽  
Average Body Weight

Empirical evidence of complementary medicine from one community in Kalimantan often uses Ulin fruit (Eusideroxylon zwageri) for medical therapy or as herbal drinks by people with diabetes mellitus. This research aims to identify the antidiabetic compound of Ulin fruit extract against Streptozotocin (STZ) induced diabetic rats. Extraction was performed by maceration method using 96% ethanol, phytochemical test of extracts, extract toxicity test on mice (50 mg/BW to 200 mg/BW), test antidiabetic extract in diabetic rats induced by STZ as much as 70 mg/kgBW with each extract dose of 150, 300, and 600 mg given for 14 days, positive control is Glibenclamide 0.25 mg/kg/day, and the negative control is CMC 1%. Phytochemical test results showed that the extract of Ulin contains alkaloids, tannins, flavonoids, phenolics, terpenoids, and steroids. Ulin fruit extract was not toxic to rats at 50, 150, and 200 mg for three days. Based on the Mann-Whitney test, there was an effect of giving the extract on changes in the average body weight and body temperature of rats during three days of administration (p = 0.000). The results showed novelty that the extract of Ulin fruit affected reducing blood glucose levels in diabetic rats during 14 days of administration (p=0.000).Keywords: Antidiabetic, Eusideroxylon zwageri, flavonoids, streprozotocin, diabetic rats

Cocos nucifera L. oil alleviates lead acetate-induced reproductive toxicity in sexually-matured male Wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Olugbemi T. Olaniyan ◽  
Olakunle A. Ojewale ◽  
Ayobami Dare ◽  
Olufemi Adebayo ◽  
Joseph E. Enyojo ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Cocos Nucifera ◽  
Reproductive Toxicity ◽  
Positive Control ◽  
Negative Control ◽  
Male Wistar Rats ◽  
Cocos Nucifera L

Abstract Objectives Lead primarily affects male reproductive functions via hormonal imbalance and morphological damage to the testicular tissue with significant alteration in sperm profile and oxidative markers. Though, different studies have reported that Cocos nucifera L. oil has a wide range of biological effects, this study aimed at investigating the effect of Cocos nucifera L. oil on lead acetate-induced reproductive toxicity in male Wistar rats. Methods Twenty (20) sexually matured male Wistar rats (55–65 days) were randomly distributed into four groups (n=5). Group I (negative control)—distilled water orally for 56 days, Group II (positive control)—5 mg/kg bwt lead acetate intraperitoneally (i.p.) for 14 days, Group III—6.7 mL/kg bwt Cocos nucifera L. oil orally for 56 days and Group IV—lead acetate intraperitoneally (i.p.) for 14 days and Cocos nucifera L. oil for orally for 56 days. Rats were sacrificed by diethyl ether, after which the serum, testis and epididymis were collected and used for semen analysis, biochemical and histological analysis. Results The lead acetate significantly increases (p<0.05) testicular and epididymal malondialdehyde (MDA) levels, while a significant reduction (p<0.05) in sperm parameters, organ weight, testosterone and luteinizing hormone was observed when compared with the negative control. The coadministration of Cocos nucifera oil with lead acetate significantly increases (p<0.05) testosterone, luteinizing hormone, sperm parameters and organ weight, with a significant decrease (p<0.05) in MDA levels compared with positive control. Histological analysis showed that lead acetate distorts testicular cytoarchitecture and germ cell integrity while this was normalized in the cotreated group. Conclusions Cocos nucifera oil attenuates the deleterious effects of lead acetate in male Wistar rats, which could be attributed to its polyphenol content and antioxidant properties.

scholarly journals Growth Performance, Serum Biochemical Indices, Duodenal Histomorphology, and Cecal Microbiota of Broiler Chickens Fed on Diets Supplemented with Cinnamon Bark Powder at Prestarter and Starter Phases

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  
Keyword(s):  
Body Weight ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Positive Control ◽  
Negative Control ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Serum Globulin ◽  
Maximum Improvement

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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