scholarly journals POTENSI EKSTRAK BUNGA ROSELLA (Hibiscus sabdariffa L.) SEBAGAI PEWARNA DAN PENGAWET ALAMI PADA JELLY JAJANAN ANAK

Heuristic ◽  
2016 ◽  
Vol 11 (02) ◽  
Author(s):  
Kejora Handarini
Keyword(s):  
Antibacterial Activity ◽  
Natural Dye ◽  
Plate Count ◽  
Hibiscus Sabdariffa ◽  
Food Grade ◽  
Total Coliforms ◽  
A Value ◽  
Total Plate Count ◽  
Best Value ◽  
Microbial Contaminants

Jelly is a highly preferred snack of children, but was reported that jelly products sometimes containing a non food grade dyes and harmful preservatives, whict is’s used for textile processed usually. Rosella flower extracts was containing antibacterial activity such as alkaloid, saponin tanin, and high anthocyanin that could be nature dye. The purpose of this study is to determine how the optimal concentration of roselle flowers extracts can be applied to jelly as a natural dye and preservatives. The methods were: a. extraction of rosella flower. b. application of an extract rosella flower onto the jelly product (the treatments are A0: 0 %A1 : 0.5 % , A2 : 1 % , A3 : 1.5 %) and then storage at refrigeration temperature for 0, 5, and 10 days .Parameters measured were the Total Plate Count (TPC), total mold-yeasts, total coliforms, and product color observations. The results showed for the storaging in 0 to 5 days, roselle jelly with various concentrations have indigenous microbial contaminants (the TPC, mold- yeasts and coliforms) is smaller than the control ( A0: 0% ). While the 10-day storaged, the TPC values , molds - yeasts and coliforms in jelly with roselle extracted was lower than controls, but for all treatments had an exceeding value than the microbiological standards. The results of colour with organoleptic test showed for 0 to 5 days of storage refrigerator, showed that roselle jelly A3 ( 1.5 % ) has the best value compared to other treatments, the value of a little love to love ( and its value is higher than controls). On the 10th day of storaged, for the treatment of roselle jelly A1, and A2 has a value of color under control, while the A3 is equal to the control. Based on the microbiological and organoleptic tests, the best roselle jelly is A3 sample and 5 days storaged in the refrigerator.Kata Kunci: Rosella, pengawet alami, pewarna alami, jelly

scholarly journals KUALITAS PERMEN JELLY SARI BUAH PEPAYA (Carica papaya L.) DENGAN PENAMBAHAN EKSTRAK KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 26
Author(s):  
Livia Adriana ◽  
Franciscus Sinung Pranata ◽  
Yuliana Reni Swasti
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Carica Papaya ◽  
Plate Count ◽  
Hibiscus Sabdariffa ◽  
Total Anthocyanin ◽  
Total Plate Count ◽  
Randomized Design ◽  
Completely Randomized Design

Jelly candy is a favorite snack of various ages from children to adults because it has an attractive color, delicious aroma and taste, and a chewy texture. In this study, papaya juice (Carica papaya L.) was used as a base ingredient for making jelly candy because papaya is a fruit that is very easy to find and has a relatively cheap price. In order to improve the quality of the papaya jelly candy, roselle calyx extract (Hibiscus sabdariffa L.) was also added. Roselle calyx has been known to have high antioxidants because they contain anthocyanin pigments. The purpose of this study was to determine the effect of variations in the addition of roselle calyx extract to the papaya jelly candy. The experimental design in this study was a completely randomized design with variations in the addition of roselle calyx extract by 0% (control), 15%, 30%, and 45%. The parameters tested in this study include chemical, physical, microbiological, and organoleptic qualities. The results of the research that have been carried out show the addition of 45% roselle calyx extract can produce the best papaya jelly candy based on water content, total titrated acid, antioxidant activity, total anthocyanin, texture, color, and microbiological tests which include total plate count and numbers of yeast molds. Keywords : jelly candy, papaya juice, roselle calyx extract

Microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with food grade commercial chemicals

2015 ◽  
Author(s):  
Vikas Kumar ◽  
D. Sukumar ◽  
M. Muruganantham
Keyword(s):  
Quality Evaluation ◽  
Microbiological Quality ◽  
Plate Count ◽  
Microbial Quality ◽  
Food Grade ◽  
E Coli ◽  
Total Plate Count ◽  
Sepioteuthis Lessoniana ◽  
Better Than

Squids perish rapidly like other fishes and require some treatment to maintain the quality for export. The microbial quality of frozen squid (Sepioteuthis lessoniana, Lesson 1830) treated with the commercial food grade chemicals imported from Spain was therefore studied. Dressed squids were treated with the chemicals Hidratech_4A (0.4%) and Whitech_3 (0.25%) dissolved in chilled freshwater (STCF) and chilled saltwater (STCS). Chilled squids not treated served as control (SNTC). Squids were quick frozen at -40°C in contact plate freezer and stored at-20±1°C. Samples were tested raw, after pre-processing, treatments and freezing and during storage at monthly intervals for 7 months. The microbial quality evaluation included Total Plate Count (TPC), Escherichia coli, Staphylococcus aureus, Vibrio cholerae and Salmonella. Study revealed a better quality of treated samples than control. Microbiological quality of STCS was better than STCF and SNTC. E. coli counts decreased after treatment. Salmonella and V. cholerae were absent.

scholarly journals A Study on Standard of Buffalo Meat Hygiene in Dharan

2014 ◽  
Vol 7 ◽  
pp. 99-101 ◽  
Author(s):  
Bhaskar Mani Adhikari ◽  
Rajendra Prasad Subedi ◽  
Dilip Subba
Keyword(s):  
Staphylococcus Aureus ◽  
Field Survey ◽  
Plate Count ◽  
Survey Method ◽  
Total Coliforms ◽  
E Coli ◽  
Buffalo Meat ◽  
Total Plate Count ◽  
Microbiological Analyses

Hygiene standard of buffalo meat in Dharan was assessed by microbiological analyses and field survey method. Ten samples of buffalo meat, knives, chopping board and hands of butchers were examined for total plate count (TPC), total coliforms, E. coli, Staphylococcus aureus, Salmonella and Shigella. Average of TPC, Coliforms, E. coli and S. aureus counts in meat were 3.59×107, 2.06×104, 1.69×103 and 9.67×103 cfu/g respectively. Salmonella was detected in 80% samples and all samples were Shigella positive. The average TPC count of chopping board, knives and hands were found to be 3.15×104, 3.47×103 and 2.01×104 cfu/ cm2 respectively. The average Coliform, E. coli and S. aureus counts of chopping board were found to be 1.11×103, 9.8× 101 and 6.2×102 cfu/cm2. The average Coliform, E. coli and S. aureus counts of knives were found to be 1.31×103, 1.66×102 and 2.83×102 cfu/cm2. The average Coliform, E. coli and S. aureus counts of the palms of butchers were found to be 1.95×103, 1.66×102 and 1.77×102 cfu/cm2. Two chopping boards, three knives and three hands were found Salmonella free. Five chopping boards, three knives and two hands were detected for Shigella. The field survey of 31 meat shops showed that the hygiene condition of meat sold in Dharan was found unsatisfactory. DOI: http://dx.doi.org/10.3126/jfstn.v7i0.10617   J. Food Sci. Technol. Nepal, Vol. 7 (99-101), 2012  

scholarly journals The Effect of Turmeric Extract (Curcuma Domestica Val) Against the Durability of Yellow Rice Storage

2019 ◽  
Vol 2 (1) ◽  
pp. 30-36
Author(s):  
Hetty Lendora Maha ◽  
Erly Sitompul ◽  
Jansen Silalahi
Keyword(s):  
Antibacterial Activity ◽  
Shelf Life ◽  
Microbial Growth ◽  
Plate Count ◽  
Traditional Food ◽  
Extract Concentration ◽  
Cooked Rice ◽  
Total Plate Count ◽  
Turmeric Extract ◽  
Plate Count Method

Yellow cooked rice is a traditional food in some regions in Indonesia. Yellow cooked rice with the addition of turmeric and flavoring which can longer shelf life of savings because it has antibacterial activity and flavor. The purpose of this study was to determine the effect of turmeric extract (Curcuma domestica Val) to extend shelf life of yellow cooked rice. The samples used were made of yellow cooked rice with handmade and without any seasoning flavored with turmeric extract concentration of 1.8% and 2.4%. Organoleptic testing were conducted every 3 hours, test of microbial growth daily and inhibition of turmeric extract with a concentration of 1.8% and 2.4% of fungi isolated from yellow cooked rice with a total plate count method. Research showed that turmeric extract concentration and the addition of  herbs affect the longer shelf life of yellow cooked rice store. High more and more  concentration of the turmeric extract can made longer the shelf life of yellow cooked rice either through organoleptic and also total plate count. Yellow cooked rice without seasoning with turmeric extract concentration of 1.8% and 2.4% were 30 and 33 hours respectively, while the yellow cooked rice was flavored with the same concentration of turmeric extract  were 36 and 39 hours respectively.

scholarly journals Antioxidant Activity and Consumer Preference of Brown Algae Sargassum hystrix Juice as a Functional Drink

2021 ◽  
Vol 41 (3) ◽  
pp. 231
Author(s):  
Punky Kusuma Damayanti ◽  
Amir Husni ◽  
Siti Ari Budhiyanti
Keyword(s):  
Antioxidant Activity ◽  
Consumer Preference ◽  
Total Sugar ◽  
Activity Level ◽  
Plate Count ◽  
Total Phenolic ◽  
A Value ◽  
Total Plate Count ◽  
Phenol Water ◽  
Functional Drink

Sargassum hystrix juice is a functional beverage made from S. hystrix through the immersion process, crushing, filtering, and pasteurization. Therefore, this study aims to determine the effect of S. hystrix concentration on antioxidant activity, level of consumer acceptance, and chemical composition of the juice. The fresh S. hystrix used were washed, immersed for three days, and blended at a concentration of 10, 20, 30, 40, and 50%, respectively. Meanwhile, the qualities of juice that were observed include yield, antioxidant activity, total phenol, water, ash, protein, and fiber content, formol number, total sugar, total plate count, and sensory analysis. The results showed that the S. hystrix juice had a value of yield 43.00-85.67%, pH 7.5, antioxidant activity 20.50-44.57%, total phenolic 32.78-293.11 mg GAE/g, water 93.03-95.39%, ash 0.36-6.34%, protein 0.88-4.96%, crude fiber 0.41-5.04%, formol number 15-21.67 mL N NaOH/100 mL, total sugar 3.10-4.61%, total plate count 2.3x103-4.3x103CFU/mL, sensory of color 1.83-2.95, flavor 2.49-3.29, and taste 2.29-3.50.

scholarly journals Antibacterial activity of taro (Colocasia esculenta L.) leaves extracts against Staphylococcus aureus and Vibrio parahaemolyticus and its effect on microbial population in sardine (Sardinella longiceps Val.)

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 88-97
Author(s):  
K.N.M. Padzil ◽  
N.M. Ayob ◽  
A.M. Alzabt ◽  
Y. Rukayadi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Vibrio Parahaemolyticus ◽  
Microbial Population ◽  
Inhibition Zone ◽  
Colocasia Esculenta ◽  
Plate Count ◽  
Time Curve ◽  
Total Plate Count ◽  
Diffusion Assay

Colocasia esculenta L. which is popularly known in Malaysia as ‘‘keladi’’ has extensive traditional uses for certain of the plant part such as the leaves, corm and stem. An earlier study has reported on the analysis of its pharmacological properties such as antiinflammatory and antibacterial activity. This study aimed to determine the antibacterial activity of C. esculenta L. leaves extracts against Staphylococcus aureus ATCC 29737 and Vibrio parahaemolyticus ATCC 17802 and its effect on the microbial population of sardine. The extract was tested for antibacterial activity against S. aureus and V. parahaemolyticus in term of disc diffusion assay (DDA), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and killing time curve by using Clinical and Laboratory Standard Institute (CLSI) methods. DDA assay of the extract resulted in 8.40±0.10 mm and 8.20±0.16 mm of inhibition zone of the extract on S. aureus and V. parahaemolyticus, respectively. The extract can inhibit the growth of S. aureus and V. parahaemolyticus with MICs values of 1.25±0.00 and 0.31±0.00 mg/mL and can kill the bacteria with MBCs values of 2.50±0.00 and 0.63±0.00 mg/mL, respectively. The killing time analyses showed that the S. aureus and V. parahaemolyticus can be killed completely for 4 hrs at 2 × MIC (25.0 mg/mL) and 2 × MIC (6.25 mg/mL), respectively. Application of the extract on sardine samples shows significant reduction in total plate count (TPC), S. aureus and V. parahaemolyticus count after treated with 5.00% of the extract and stored at 4˚C for 30 mins. Based on the findings, it indicates that C. esculenta L. can be listed as one of the alternatives natural sanitiser or preservative agent to reduce the bacterial loading of raw sardine prior to cooking

scholarly journals Implementasi Higiene Sanitasi pada RPH Kategori I sebagai Syarat Produksi Daging Sehat

2021 ◽  
Vol 9 (3) ◽  
pp. 138-143
Author(s):  
M. Fitri ◽  
H. Nuraini ◽  
R. Priyanto ◽  
Y. C. Endrawati
Keyword(s):  
Water Quality ◽  
Quality Assurance ◽  
Assessment Method ◽  
Personal Hygiene ◽  
Plate Count ◽  
E Coli ◽  
A Value ◽  
Total Plate Count ◽  
Sanitation And Hygiene ◽  
Quality Testing

Abbatoirs have to produce meat that complied requisite of safety, healthy, wholeness and halal. Animalorigin product have to requisite basic safety product which implementated of hygine sanitation asproduction processed. The research aim was to evaluate implementation of hygine sanitation systemon 1st categrory abbatoir at Bogor Regency to complied safety and quality assurance of product.Observations on the implementation of hygienic sanitation were carried out using an evaluation matrixof the suitability of physical requirements with the criterion parameter assessment method based onthe Regulation of the Minister of Agriculture Number 13/2010. The best suitability value (NK) of 3 wasobtained on the environmental hygiene indicator and the absence of the use of chemicals, while theother sanitation hygiene indicators were worth 2 (less appropriate). The results of testing on meat forthe content of Salmonella sp. is negative and the Total Plate Count test is between 7.1 x 103 - 5.2 x 104cfu/g below the SNI threshold (1.0 x 106 cfu/g). The test for E. coli in meat was above the threshold ofsuspected contamination from the water used. Validation of hygiene sanitation practices was carried outby means of Colliform swabs on knives, palms hand and clothing of officers. Colliform swab test of theofficer’s palm hand, which is 5.4 x 102 cfu/g, shows a value above the allowable threshold. The results ofthe evaluation of the application of sanitation and hygiene in the abattoir are quite good but still needimprovement. Priority improvements are the equipment of cleaning facilities, water quality testing andimprovement of personal hygiene understanding.

Pengaruh Suhu Penyimpanan terhadap Organoleptik, Derajat Keasaman dan Pertumbuhan Bakteri Coliform pada Susu Pasteurisasi

2016 ◽  
Vol 10 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Agustina Arianita Cahyaningtyas ◽  
Wiwik Pudjiastuti ◽  
Ilham Ramdhan
Keyword(s):  
Storage Temperature ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Pasteurized Milk ◽  
Initial Analysis ◽  
Total Plate Count ◽  
Plate Count Method ◽  
Pathogenic Microbes ◽  
Total Coliform Bacteria

One attempt to reduce the number of pathogenic microbes in milk is through the pasteurization process. This research aims to determine the effect of storage temperature on the organoleptic, acidity (pH) and growth of coliform bacteria in pasteurized milk. Pasteurized milk is stored at the varies of temperature  4°C (observed for 14 days), 10°C-15°C (observed for 14 days) and 25°C-27°C (observed for 22 hours), as well as also conducted an initial analysis pasteurized milk. The parameters were observed among other organoleptic (smell, taste, color, texture), pH and total coliform bacteria. Testing acidity using pH paper, while the growth of coliform bacteria testing done using Total Plate Count method based on ISO 2897 in 2008. The results of this study indicate that storage at 4°C for 14 days, organoleptic pasteurized milk is still good until the day ke- 8, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 3100x101 CFU / ml. Storage at 10°C-15°C for 14 days, organoleptic pasteurized milk is still good until the 6th day, the pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 5729x101 CFU / ml. Storage at 25°C-27°C for 22 days, organoleptic pasteurized milk is still good until the 9th, pH progressively decreases, and the growth of coliform bacteria obtained the highest score of 4.3 x106 CFU / ml.ABSTRAKSalah satu usaha untuk mengurangi jumlah mikroba patogen pada susu adalah melalui proses pasteurisasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap organoleptik, derajat keasaman (pH) dan pertumbuhan bakteri Coliform pada susu pasteurisasi. Susu pasteurisasi disimpan pada suhu yang bervariasi yaitu suhu 4°C (diamati selama 14 hari), suhu 10°C-15°C (diamati selama 14 hari) dan suhu 25°C-27°C (diamati selama 22 jam), serta dilakukan pula analisa awal susu pasteurisasi. Parameter yang diamati antara lain organoleptik (bau, rasa, warna, tekstur), pH dan jumlah bakteri Coliform. Pengujian derajat keasaman menggunakan kertas pH, sedangkan pengujian pertumbuhan bakteri Coliform dilakukan dengan menggunakan metode Total Plate Count berdasarkan SNI 2897 Tahun 2008. Hasil penelitian ini menunjukkan bahwa penyimpanan pada suhu 4°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai dengan hari ke-8, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 3100x101 Cfu/ml. Penyimpanan pada suhu 10°C-15°C selama 14 hari, organoleptik susu pasteurisasi masih baik sampai hari ke-6, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 5729x101 Cfu/ml. Penyimpanan pada suhu 25°C-27°C selama 22 hari, organoleptik susu pasteurisasi masih baik sampai jam ke-9, pH semakin lama semakin menurun, dan pertumbuhan bakteri Coliform didapatkan nilai tertinggi sebesar 4,3 x106 Cfu/ml.Kata kunci : bakteri coliform, derajat keasaman, suhu penyimpanan, organoleptik, susu pasteurisasi

Analisis Kandungan Mikroba, Formalin, dan Timbal (Pb) pada Tahu Sumedang yang Dijual Di Daerah Macet Cicurug, Ciawi, dan Cisarua Jawa Barat

2020 ◽  
Vol 6 (1) ◽  
pp. 087
Author(s):  
Rosy Hutami ◽  
M Fakih Kurniawan ◽  
Henna Khoerunnisa
Keyword(s):  
Content Analysis ◽  
Microbial Contamination ◽  
Atomic Absorption Spectrophotometry ◽  
Plate Count ◽  
Traffic Jam ◽  
Absorption Spectrophotometry ◽  
Traffic Jams ◽  
Total Plate Count ◽  
Microbial Analysis ◽  
Aas Method

Sumedang tofu is one of favorite foods for Indonesian society. But many sellers or producers are not aware to the food safety of sumedang tofu. The aims of this study were to analyze the microbial, formalin, and lead (Pb) contents in ready-to-eat sumedang tofu which were sold in traffic jams area in Cicurug, Ciawi, and Cisarua. The analysis were carried out by Total Plate Count (TPC) testing for microbial analysis, potassium permanganate reaction (KMnO4) testing for formaldehyde analysis, and atomic absorption spectrophotometry (AAS) method for lead content analysis in the samples. The results obtained for the microbial analysis were sumedang tofu that were sold in the traffic jam areas of Cicurug, Ciawi, and Cisarua contained contaminant above the treshold (1.4 x 105 colonies / gram to 2.2 x 105 colonies / gram of microbes). All of the samples of sumedang tofu were positive containing formaldehyde. Otherwise, there were no lead (Pb) content in all samples regarding to AAS analysis. This study concluded that the ready-to-eat sumedang tofu those were sold in traffic jam area in the Cicurug, Ciawi, and Cisarua were not suitable for consumption because it contained exceed microbial contamination and formalin which are harmful for human health.Keywords : formalin, microbes, sumedang tofu, lead, traffic jam

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