EFFECT OF NICKEL AND ITS INSOLUBLE INORGANIC COMPOUNDS ON REPRODUCTIVE FUNCTION OF WHITE RATS

2021 ◽  
Author(s):  
L.V. Lukovnikova ◽  
◽  
L.A. Lelbiks ◽  
E.E. Lesiovskaya
Keyword(s):  
Average Length ◽  
Inorganic Compounds ◽  
Experimental Studies ◽  
Reproductive Function ◽  
Mature Female ◽  
Intrauterine Death ◽  
Breathing Zone ◽  
The European Union ◽  
White Rats ◽  
Nickel Compounds

Abstract. Introduction. The problem of women's health protection in enterprises producing and using nickel and its compounds is particularly relevant, since women make up a significant contingent of workers at some stages of production. Nickel and its compounds are used in the production of high-alloy steels, alloys with copper, chromium, aluminum, as a catalyst in the processes of hydrogenation of fats, in the production of batteries, nickel-plating of metal products. In case of violations of the technological process, labor protection conditions, workers may be exposed to nickel aerosol and its oxides. Purpose. To study the effect of nickel and its insoluble compounds on the reproductive function of white rats. Method of research. The studies were carried out on sexually mature female white rats weighing 180-200 g with a stable estrous cycle lasting 4-6 days. Experimental studies were conducted in accordance with national and international regulatory requirements ensuring humane treatment of animals used in experiments: Directive 2010/63/EU of the European Parliament and of the Council of the European Union of September 22, 2010 on the protection of animals used in scientific purposes, Order of the Ministry of Health of the Russian Federation of 01.04.2016 No. 199n «On approval of the Rules of good laboratory Practice». The industrial conditions for the action of aerosol of insoluble nickel compounds were simulated in a special chamber with an individual intake of aerosol into the breathing zone at a concentration of 0.2 mg / m3 daily for 4 hours during the entire gestation period. The animals were assessed for the dynamics of body weight on the 1st, 8th, 14th and 20th days of pregnancy. On the 20th day of pregnancy, the number of yellow bodies of pregnancy, placentas and fetuses was determined, pre-, post-implantation and total intrauterine death, average length and weight of fetuses, and average placenta weight were calculated. The total number of pregnant females and fetuses was recorded with the subsequent calculation of the number of fetuses per female. Results. The action of an aerosol of insoluble nickel compounds at a concentration of 0.2 mg / m3 in this mode did not lead to a violation of the reproductive function of white rats in all the studied parameters. Conclusion. The experimental data obtained indicate that insoluble nickel compounds at the level of the maximum permissible concentrations for the air of the working area will not pose a risk of developing reproductive health pathology in working women.

EVALUATION OF TOXIOLOGICAL INDICATORS OF MONTHMORRILLONITE CLAY FOR THE PURPOSE OF USING THEM AS ALUMOSILICATE SOBENT AGAINST THE NEGATIVE EFFECT OF MYCOTOXINS ON THE BODY OF ANIMALS AND BIRDS

2021 ◽  
Vol 1 (1) ◽  
pp. 72-78
Keyword(s):  
Experimental Studies ◽  
The Body ◽  
Montmorillonite Clay ◽  
Test Substance ◽  
Negative Effects ◽  
Experimental Animals ◽  
Eyelid Edema ◽  
White Rats ◽  
Mucous Membranes ◽  
Negative Effect

The article presents the results of laboratory studies to assess the toxicological parameters of montmorillonite clay from the Pogadaevskoye deposit in the West Kazakhstan region in order to use them as an aluminosilicate sorbent in the composition of feeds that reduce the negative effects of mycotoxins on the body of animals and birds. The relevance of research is associated with the cultivation of healthy and highly productive animals and poultry in order to ensure the food security of the Republic of Kazakhstan.The studies carried out to assess the toxicological parameters of montmorillonite clay in order to use them as an aluminosilicate sorbent in experimental animals (rabbits and white rats) allowed the following results to be obtained: Visual study of the intensity of erythema when exposed to the test substance on the skin of rabbits showed their absence (0 points). The study of the intensity of edema (an increase in the thickness of the skin clutch of rabbits) when exposed to the test substance on the skin of rabbits showed no reaction (0 points). Studies evaluating the irritating effect of the test substances on the mucous membranes of the eyes of rabbits by symptoms of damage showed the absence of hyperemia (0 points). Weak eyelid edema (1 point), the minimum amount of discharge in the corner of the eye (1 point). The results of studies on the classification assessment of the test substance for the severity of the irritant effect on the eyes of rabbits showed that the average total score of the severity of the irritative effect corresponds to 1 point. A comprehensive analysis of the results obtained on the basis of scientific and experimental studies to assess the toxicological indicators of montmorillonite clay from the Pogadaevskoye deposit in relation to irritating effects on the skin and mucous membranes of experimental animals (rabbits) showed their harmlessness.

scholarly journals EVALUATION OF TOXIC EFFECTS OF MAGNETIC CONTRAST DIAGNOSTIC GADOLINIUM-CONTAINING NANOCOMPOSITE

2019 ◽  
Vol 98 (10) ◽  
pp. 1161-1165
Author(s):  
Larisa M. Sosedova ◽  
E. A. Titov ◽  
M. A. Novikov ◽  
V. A. Vokina ◽  
V. S. Rukavishnikov
Keyword(s):  
Nervous Tissue ◽  
Chemical Elements ◽  
Biological Effects ◽  
Experimental Studies ◽  
Biological Response ◽  
Immunohistochemical Method ◽  
Ether Anesthesia ◽  
Compensatory Response ◽  
White Rats ◽  
Liver And Kidney

Introduction. In recent years, magnetic nanoparticles, which can simultaneously have a therapeutic effect on the pathological focus, are used to magnify contrast enhancement and increase diagnostic sensitivity during magnetic resonance therapy (MRT). The last is carried out by the effective capture of neutrons, which among all the chemical elements is most pronounced in gadolinium. The use of gadolinium nanoparticles encapsulated in a polymeric matrix allows increasing the bioavailability of nanoparticles, reduces the possible toxicity of drugs. Aim. Evaluation of impact of new nanocomposite magnetically active metal complex gadolinium system on the morphofunctional state of the nervous tissue, liver, and kidney of rats. Material and methods. Experimental studies of biological effects of gadolinium-arabinogalactan nanocomposite (Gd-AG) were carried out on rats that were injected intraperitoneally for 10 days at the dose of 500 μg/kg in 0.5 ml of saline. Animals were sacrificed by decapitation under light ether anesthesia the next day after the end of exposure. To perform pathological studies, frontal sections of the temporal-parietal zone of the sensorimotor cortex, liver and kidney tissues were stained on ordinary histological glass slides with hematoxylin and eosin for viewing microscopic picture. The immunohistochemical method was used to determine the activity of the bcl-2, caspase-3 and hsp70 modulatory protein in apoptosis of white rats in brain neurons and to study the biological response of the organism at the subcellular level. Results. Histological analysis of tissues revealed a pronounced compensatory response of liver, a violation of the functional activity of kidneys. A decrease in the total number of normal neurons per unit area in brain tissue and an increase in the number of acts of neuronophagy indicate the initial stage of neurodegenerative process. Evaluation of the intracellular metabolism of neurons has not established the presence of signs characteristic of apoptotic process. Conclusion. The subacute effect of Gd-AG in a dose of 500 µg/kg causes a disturbance of morphofunctional state of liver, kidneys and nervous tissue, as well as modulation of cellular proteomics.

Experimental Study of Air Distribution Characteristics in Room of Stratum Ventilation under Two Kinds of Air Change Times

2013 ◽  
Vol 409-410 ◽  
pp. 668-672
Author(s):  
Yong Mei Xu ◽  
Jian Tang ◽  
Jun Han ◽  
Chu Qin Lin
Keyword(s):  
Experimental Study ◽  
Thermal Comfort ◽  
Experimental Studies ◽  
Air Distribution ◽  
Distribution Characteristics ◽  
Breathing Zone ◽  
Wind Speeds ◽  
Pollutant Concentrations ◽  
New Type ◽  
Better Than

Aimed at a new type of ventilation - stratum ventilation, air distributions at a breathing-zone in a model office were measured under kinds of air changes, the measure parameters in the experimental studies included temperatures, wind speeds and pollutant concentrations, based on which the thermal comfort at a breathing-zone were studied. Experimental results show that, the temperature, pollutant concentration and wind speeds in a breathing-zone under 5 times air changes are better than those under 6 times air changes. The calculating results of PMV and PPD indicate that the thermal comfort at a breathing-zone under 5 times air changes is better. The experimental study is instructive for the development of the ventilation.

The pig producer's perspective

1999 ◽  
Vol 23 ◽  
pp. 1-5
Author(s):  
J. Guise
Keyword(s):  
Management Practices ◽  
Control Measure ◽  
Experimental Studies ◽  
Effective Control ◽  
Committee Report ◽  
The European Union ◽  
Tail Biting ◽  
Farming Community ◽  
Tail Docking ◽  
The Uk

AbstractThis paper is designed to demonstrate that practical, applied research, which is popular with the farming community, is an important part of the decision-making process. Three recent or current projects are described in areas where strategic and basic science has made an important contribution to the debate but has not provided solutions. Confinement at farrowing, tail biting and fully slatted finishing systems are all areas of concern to producers and legislators alike and these concerns are not confined to the UK. The results of surveys of industrial practice have suggested that: (a) non-confined systems may perform as well as farrowing crates, (b) tail-docking appears an effective control measure for tail biting but more importantly, the relative influence of different management practices is measurable using the techniques described and could provide solutions to the tail-biting problem, and (c) experimental studies of finishing systems have produced ambivalent results and a new approach may be of value. The recent Scientific Veterinary Committee Report of the European Union is used extensively as the basis for the discussion.

scholarly journals Pengaruh Daun Tapak Kambing (Ipomoae pes-caprae) Terhadap Konsentrasi Spermatozoa Tikus Putih (Rattus norvegicus)

2019 ◽  
Vol 11 (2) ◽  
pp. 95
Author(s):  
Kaspul Kaspul
Keyword(s):  
Randomized Trial ◽  
Rattus Norvegicus ◽  
Trial Design ◽  
Reproductive Function ◽  
Reproductive Capacity ◽  
Antioxidant Compounds ◽  
Reproductive Disorders ◽  
Treatment Groups ◽  
White Rats ◽  
All Treatment

Reproductive continuity is an important problem for humans and animals, but sometimes reproductive disorders occur which results in decreased reproductive capacity. Goat’s foot creeper leaves have active compounds that act as antioxidants. Antioxidant compounds can maintain reproductive function. This study aims to determine the effect of Goat’s foot creeper leaves on the spermatozoa concentration of white rats. Twenty white rats male (4-week-old) were treated with a completely randomized trial design. There were 4 treatment groups with 5 replications: (1) no treatment; (2) treatment with 750 mg goat’s foot creeper infusion per day; (3) treatment with 1500 mggfoot creeper leaves per day. All treatment groups were administered for 30 days orally. The results showed an increase in spermatozoa concentration of white rats.

scholarly journals Heterozygous Eif4nif1 Stop Gain Mice Replicate the Primary Ovarian Insufficiency Phenotype

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A774-A775
Author(s):  
Mika Moriwaki ◽  
Corrine Kolka Welt
Keyword(s):  
Mouse Model ◽  
Litter Size ◽  
Average Length ◽  
Reproductive Function ◽  
Protein Translation ◽  
Antral Follicle ◽  
Primary Ovarian Insufficiency ◽  
Primary Follicle ◽  
Ovarian Insufficiency ◽  
Exon 10

Abstract We identified a stop-gain mutation in eIF4ENIF1 in a family in which multiple women developed primary ovarian insufficiency (POI) at approximately age 30 years. We hypothesized that the same mutation in a mouse model would replicate POI. Methods: The Eif4enif1 C57/Bl6 transgenic mouse model contains a floxed exon 10-19 cassette and a conditional knock-in cassette containing exon 10 with the c.1286C>G stop-gain mutation causing familial POI and WT exons 11-19 (Eif4enif1WT/flx). The hybrid offspring of CMV-Cre mice with Eif4enif1WT/flx mice were designated Eif4enif1WT/Δ for simplicity. Follicles were counted in fixed H&E stained ovaries from mice age days 1-5 (primordial and primary follicles), day 10, day 22 (first wave of growing follicles from small preantral to small antral follicles), week 20 (peak fertility), then every 2 months from 10 months to 26 months (follicle exhaustion). Litter frequency, pup number and genotype were recorded. Serum FSH levels were measured by the University of Virginia Ligand Assay and Analysis Core. Results: The heterozygotes have no outward or internal phenotypic differences compared to WT (Eif4enif1WT/flx), with the exception of reproductive organs in females and males. A subset of female heterozygotes (Eif4enif1WT/Δ) had no litters for 20 weeks (2 of 18; 11%). In those with litters, the average length of time between litters was not different but the final litter was earlier (5.6±2.7 vs. 10.5±0.7 months; p=0.02). Heterozygous breeding pair (Eif4enif1WT/Δx Eif4enif1WT/Δ) litter size was 60% of WT litter size (3.9±2.3 vs. 7.2±2.1 pups/litter; 0<0.001). The genotypes were 35% Eif4enif1WT/flx and 65% Eif4enif1WT/Δ, with no homozygotes. The number of follicles in ovaries from Eif4enif1WT/Δ mice was lower starting at the primordial (499±290 vs. 1445±381) and primary follicle stage (1069±346 vs. 1450±193) on day 10 (p<0.05). The preantral follicle number was lower starting on day 21 (213±86 vs. 522±227; p<0.01) and the antral follicle count was lower starting on week 20 (78±38 vs. 119±18; p<0.01). The FSH level in 12-month old mice during estrus was higher in a heterozygote compared to WT (25.0 vs. 12.1 ng/mL). Conclusions: Heterozygous Eif4enif1 stop-gain mutants have follicle loss documented by day 10, decreased pup number with no homozygotes, earlier end of reproductive function and elevated FSH levels. These mice replicate the POI phenotype in women. eIF4ENIF1 regulates protein translation by binding and storing eIF4E bound mRNA. Therefore, the unique mouse model provides a platform to study temporal and spatial regulation of protein translation across oocyte and embryo development in mammals. Further studies will determine whether follicle loss results from premature protein translation in oocytes.

scholarly journals ESTIMATION OF REPRODUCTIVE TOXICITY OF THE COMPLEX PLANT REMEDY �TIREOTON�

2016 ◽  
Vol 1 (2) ◽  
pp. 0-0
Author(s):  
������ ◽  
Iraida Ubeeva ◽  
�������� ◽  
Sergey Nikolaev ◽  
��������� ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Wistar Rats ◽  
Reproductive Toxicity ◽  
Reproductive Function ◽  
Negative Influence ◽  
Laboratory Animals ◽  
White Rats ◽  
Embryo Toxicity ◽  
Probable Occurrence

To study the probable negative influence of the complex remedy �Tireoton� on the reproductive function of the laboratory animals the following tests have been carried out, namely the probable occurrence of embryo-toxicity, teratogenicity, foetotoxicity and its influence on the postnatal development of the laboratory animals progeny. The experiments have been carried out of the white Wistar rats. It has been established that the �Tireoton� at the doses of 75 and 750 mg/ kg has no embryotoxic, teratogenic and fetotoxical effect. The introduction of the �Tireoton� at the dose of 750 mg/kg since 6th to 19th day of pregnancy does not influence on the postnatal development of white rats progeny.

scholarly journals STUDY OF IRRITANT AND SKIN-RESORPTIVE ACTION OF SODIUM-CONTAINING DRUG

2020 ◽  
Vol 21 (2) ◽  
pp. 105-109
Author(s):  
O. Malyk ◽  
Ya. Protsyk ◽  
N. Shkodyak ◽  
T. Yurynets ◽  
S. Martynyk ◽  
...  
Keyword(s):  
Experimental Studies ◽  
Sodium Content ◽  
Test Tube ◽  
Control Group ◽  
Potassium Sodium ◽  
Normal Limits ◽  
White Rats ◽  
Group I ◽  
Wide Range ◽  
Reliable Increase

Sodium-containing preparations have antimicrobial, anti-inflammatory and wound-healing activity, which determines their use in veterinary medicine. This effect is due to a wide range of physiological effects of sodium ions on animals metabolism. The article presents the results of experimental studies of the irritant effect of sodium-containing drugs: known – 2 % glycetinate, its analogues – 2 % ovocid, 3 % ovocid, 1.5 % potassium-sodium drug (Na / K, 1.5%). Studies of irritant and skin-resorptive action of drugs were performed by immersing the tail of animals in a test tube with the substance on white rats weighing 250–270 g. Four groups of rats were formed with 3 animals each: rats of I group (control) tails were immersed in test tubes with 2 % glycetinate, animals of II group – in test tubes with 2% oocide, III group – in test tubes with 3 % oocide, and in IV group rats – with Na / K, 1.5% drug. The exposure time was 4 hours. The animals were subjected to clinical observations, assessing their general condition and the reaction of the tail’s skin. The effect of drugs on the morphological and biochemical parameters of the rats blood was studied. The study of the local irritant effect of the studied drugs in rats from I, II and III groups showed a moderate local irritant reaction, as evidenced by the appearance of redness and thickening of the skin, and in group IV animals appeared only slight redness on the skin of the tails. This indicates a weaker local irritant effect of potassium-sodium-containing drug (1.5% K, Na), compared with other studied drugs. According to hematological studies, in the rats’s blood of groups III and IV, a reliable increase, within normal limits, the number of leukocytes and a decrease in hemoglobin content, compared with those of animals of group I. It was found that at 4 hours of the experiment under the action of the studied potassium-sodium drugs increases the permeability of Sodium and Potassium through the rats’s skin into the blood. In particular, in serum of animals from groups II and IV there was a reliable increase of Potassium content by 2.1 times, respectively, and a significant increase in Sodium content in the blood of animals from groups III and IV, compared with control group. In animals of groups II and IV there was a reliable increase of serum aspartate aminotransferase activity, and in rats of group III – increase of serum alanine aminotransferase, alkaline phosphatase activity and in triacylglycerol’s content, compared with rats of group I, which indicates the effect of 2 % glycecin analogues on metabolic processes in animals.

scholarly journals Effects of Biased Media on Foreign Policy Preferences: Evidence from a Natural Experiment

2021 ◽  
Author(s):  
Nora Kirkizh
Keyword(s):  
European Union ◽  
Foreign Policy ◽  
Natural Experiment ◽  
Experimental Studies ◽  
Policy Issue ◽  
The European Union ◽  
Public Response ◽  
The Public ◽  
Foreign Politics ◽  
Foreign Policy Issue

The expansion of globalization has made a country's prosperity increasingly dependant on foreign politics. This article shows how Russian television's inflammatory coverage of European politics affected the vote during the European Union membership referendum in Latvia. For identification, I use plausibly exogenous variation of the signal from Russian analog TV towers that was available during the referendum to Latvian counties located close to the Russian border. The analysis of the electoral data showed that in counties with the Russian television reception, votes "for" outperformed votes "against" joining the European Union compared to counties without the reception. Moreover, the effect of Russian television persisted even in counties densely populated by ethnic Russians. Contrary to previous experimental studies, the evidence suggests that specifically in the context of foreign politics, foreign biased media increase the salience of foreign policy issue but is unable to shift the direction of the public response.

scholarly journals The Effect of Black Garlic (Allium sativum Linn) on Cardiac and Aortic Histopathology in Experimental Studies in Obesity Rats

2021 ◽  
Vol 7 (2) ◽  
pp. 62-68
Author(s):  
Andreas Arie Setiawan ◽  
Fairuz Azmila Purnomo ◽  
Vega Karlowee ◽  
Noor Wijayahadi
Keyword(s):  
Body Weight ◽  
Wall Thickness ◽  
Experimental Studies ◽  
Optimal Dose ◽  
The Body ◽  
Heart Weight ◽  
Control Group ◽  
Sprague Dawley ◽  
Control Group Design ◽  
White Rats

ABSTRACTBackground: Obesity is a disorder or disease characterized by the accumulation of excess fat in the body due to an imbalance in energy intake that is used for a long time. Accumulation of fat can reduce adiponectin, causing cardiac hypertrophy, endothelial vasodilation, and other cardiovascular diseases. Black garlic have high antioxidants in the form of S-Allylcysteine(SAC) which functions to increase adiponectin. Objective: To determine the effect of Black garlic on the histopathological picture of the heart and aorta of obese rats. Methods: This study was an experimental study with a randomized post-test only design with control group design with 5 groups of male white rats Sprague Dawley (Rattus novergicus) fed High Fat Fructose. Diet (HFFD) enriched with 1.25% cholesterol and 0.5% cholic acid for 8 weeks and was given black garlic intervention at doses of 450 mg / 200BW, 900mg / 200BW and 1350mg200 / BW for 4 weeks. Results: Giving black garlic significantly reduced body weight of rats (p = 0.001), and the results did not significantly reduce heart weight (p = 0.147), aortic weight (p = 0.061), histopathological changes in heart wall thickness (p = 0.423) and aortic wall thickness (p = 0.802). The effective doses of black garlic in this study were 450 mg / 200 grams BW, 900 mg / 200 grams rat BW and 1350 mg / 200 grams BW of rats. The optimal dose is 900 mg / 200 grams BW. Conclusion: Black garlic gave a significant reduction in body weight of rats and no significant reduction in heart weight, aortic weight, cardiac and aortic histopathological features. 

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