scholarly journals CHANGES IN ULTRASTRUCTURE OF CALF MUSCLE AND MYOCARDIUM UNDER PHYSICAL LODADING IN EXPERIMENT

2020 ◽  
Vol 20 (2) ◽  
pp. 114-118
Author(s):  
V. L. Voitenko ◽  
L.M. Gunina
Keyword(s):  
Functional State ◽  
Structural Changes ◽  
Circulatory System ◽  
Physical Exertion ◽  
The Body ◽  
Calf Muscle ◽  
Male Rats ◽  
Tissue Samples ◽  
Physical Loading ◽  
Detailed Assessment

Long-term physical activity causes restructuring in the various functions and systems of the body, which are, as a rule, adaptive in nature and occur at all levels of the structural organization of the body. The question of changes in the functional state of the cardiovascular system and, especially, the microcirculatory section of the circulatory system in normal and under the physical exertion and their relationship with ultrastructural remodelling in tissues, cells and cellular organelles is still remaining unclear. The aim of the study was to assess structural changes in the tissues of the calf muscle and myocardium that cause the development of adaptive mechanisms in response to prolonged physical exertion. We studied the structural components of tissue samples of calf muscle and the apex of the heart taken from mature Wistar male rats weighing 220-250 g in mass exposed to prolonged physical exertion by using electron microscopy. It has been shown that a long and intense physical loading causes the development of a number of compensatory-adaptive reactions directed at improving microcirculation processes, oxygen supply of skeletal muscles and myocardium, as well as maintaining an adequate level of metabolic processes. A detailed assessment of the functional state of the tissues studied is necessary for the early detection of signs of maladjustment under the constant loading in sports and the prevention of pathological states.

Histopathological Changes of Organs (Lungs, Liver, Kidney, and Brain) After Using Two Types of AgiCoat and Acticoat Nanosilver Dressings on Deep Second-Degree Burn in Rat

2019 ◽  
Vol 41 (1) ◽  
pp. 141-150 ◽  
Author(s):  
Hamid Karimi ◽  
Noor-Ahmad Latifi ◽  
Ali Zare Mehrjerdi ◽  
Babak Jafarnejad ◽  
Ali-Mohammad Karimi
Keyword(s):  
Wound Healing ◽  
Silver Ions ◽  
The Body ◽  
Male Rats ◽  
Histopathological Changes ◽  
Sprague Dawley ◽  
Tissue Samples ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Prevention of infections is a very important issue in treating the burn wounds. The nanosilver dressings have many promising advantages, but absorption of silver ions and its adverse effects to the body were always a question. The aim of this study was to compare Silver serum levels and acute toxic effects of nanosilver on histopathology of organs (lungs, liver, kidney, spleen, and brain) in two types of AgiCoat and Acticoat (nanosilver) dressings on second-degree deep burn in rat. This is an experimental study conducted in our animal laboratory. We divided 24 Sprague–Dawley male rats weighing 300 to 350 randomly into two groups. After anesthesia, a second deep-degree burn was made over dorsal skins of rats by standard method. For group A, Agicoat and, for group B, Acticoat dressings were used. The dressings were changed every 3 days with AgiCoat and Acticoat, respectively. After 14 days, we got blood samples and tissue samples taken from heart, liver, kidneys, spleen, lungs, and brain and a sample from dorsal skin of the rat for histopathological examinations. The results showed that the levels of serum silver in both groups were significantly higher than the standard level (1.22 part per million (PM); AgiCoat, P = .017; Acticoat, P = .000), but there was no significant difference between the groups (P = .551). Examination of the relationship between the level of serum silver and histopathological changes in liver showed that hepatotoxicity of AgiCoat was higher compared with Acticoat and the difference was significant (P = .002). There were no pathological changes in brain, kidneys, spleen, heart, and lungs. Wound healing was faster in Acticoat group. The nanosilver dressings can cause toxicity in liver but not in kidney, brain, spleen, heart, and lungs. Liver pathology and hepatotoxicity were more prominent in AgiCoat group. Wound healing was faster in Acticoat group.

scholarly journals THE INFLUENCE OF TRAINING SESSIONS OF NORTHERN (SCANDINAVIAN) WALKING ON THE PARAMETERS OF THE FUNCTIONAL STATE OF MEDICAL UNIVERSITY STUDENTS

2021 ◽  
pp. 47-53
Author(s):  
I. V. Bocharin ◽  
M. S. Guryanov ◽  
A. K. Martusevich
Keyword(s):  
Comparative Analysis ◽  
Functional State ◽  
Physical Exertion ◽  
The State ◽  
The Body ◽  
Dynamic Changes ◽  
Adaptive Capabilities ◽  
Second Year ◽  
Medical University ◽  
And Training

In this article, the purpose of the study was a comparative analysis of the parameters of the functional state of the body of students under the systematic influence of moderate physical exertion of northern (Scandinavian) walking. The study included 85 second-year students of the Volga Research Medical University. To visualize the indicators of the state of the body, the system of sports testing “MedicalSoft” was used. The functional status of the students' organism was studied before the start of Nordic (Scandinavian) walking classes, and after the completion of a two-month course of exposure to metered physical exertion. The analysis of the data obtained was carried out in accordance with the age standards that were formed by the developers of the equipment. It is established that systematic health-improving and training loads favorably affect the state of the body of the subjects, increasing its adaptive capabilities, which is confirmed by dynamic changes in the values of integral indicators.

scholarly journals Structural changes in skeletal muscles in hypokinesia and physical loading in the posthypokinetic period of recovery of rats’ organisms

2017 ◽  
Vol 8 (2) ◽  
pp. 118-123 ◽  
Author(s):  
S. L. Popel’
Keyword(s):  
Skeletal Muscles ◽  
Structural Changes ◽  
Aerobic Power ◽  
Muscle Fibers ◽  
Male Rats ◽  
Ultrastructural Changes ◽  
Muscle Fibres ◽  
Physical Loading ◽  
Nervous Apparatus ◽  
Sexually Mature

This article reports the study of histo-ultrastructural changes of different structural components of the direct muscle of the thigh of sexually mature male rats over a prolonged period of hypokinesia and subsequent application of physical loading of average aerobic power. Using a light optical, electron microscope (for the exposure of structural transformations of muscle components) and histochemical (for determination of activity of succinate dehydrogenase according to the Nahlas method to identify muscle fibers with different phenotypes) methods, we studied the structural manifestations of adaptation of muscle fibres under prolonged (240 day) hypokinesia and 15–30 episodes of physical loading of average aerobic power in the posthypokinetic period among 55 sexually mature rats. Under prolonged hypokinesis we primarily observed changes in the intramuscular network and morphometric changes in the blood vessels. These data closely correlate with the progression of changes of the subcellular components responsible for energetic and flexible balance of muscle fibres. We found that fast oxygen-glycolytic muscle fibers and their peripheral nervous apparatus are the most sensitive to prolonged hypokinesia. As a result of application of physical loading of average aerobic power, reparative regeneration is intensified, which substantially shortens the period of recovery of structural-functional properties of skeletal muscles in the conditions of hypokinetic disorders. Thus, in prolonged hypokinesia, changes primarily affect the sources of blood supply to skeletal muscles, with the secondary development of reverse processes in muscle fibers and peripheral nervous apparatus with certain morphometric signs. 

scholarly journals INFLUENCE OF COMBINED INJECTION OF THYROXIN AND PROPYLTHIOURACILUM ON SCTRUCTURAL INDICATORS OF RENAL PARENCHYMA

2017 ◽  
Vol 21 (1) ◽  
pp. 57-67
Author(s):  
S. I. Dolomatov ◽  
V. G. Sipovski ◽  
N. Y. Novikov ◽  
I. N. Kasich ◽  
I. V. Myshko ◽  
...  
Keyword(s):  
Body Weight ◽  
Structural Changes ◽  
White Male ◽  
Kidney Tissue ◽  
Renal Parenchyma ◽  
Male Rats ◽  
Intragastric Administration ◽  
Tissue Samples ◽  
Hematoxylin And Eosin

THE AIM: to study of the dynamics of structural changes in renal parenchyma of rats exposed to long-term combined effects of thyroxine and propylthiouracilum (PTU). MATHERIAL AND METHODS – studies were performed on mongrel white male rats weighing 250-300g. Hyperthyroidism was caused by daily intragastric administration of thyroxine (T4) in amount of 50g per 100g of body weight over 30 days. On the first day of the experiment animals were divided into 2 groups. Animals of the first group (n = 25) received only T4. The rats of the second group (n = 25) were administrated propylthiouracilum and T4 daily. PTU was administered intragastric in amount of 1 mg per 100g of body weight. Kidney tissue samples were collected on the 10th, 20th and 30th days of the experiment. In addition, there were collected kidney tissue samples of the animals treated with only T4 after 20 days after cessation of hormone. Obtained tissue samples were fixed and treated by the usual method, followed by filling in paraffin. Sections were stained with hematoxylin and eosin. RESULTS – it was established that course of experimental hyperthyroidism leads to significant structural abnormalities of the renal parenchyma. Leading features of kidneys pathology at a hyperthyroidism are rough structural damages of the nephron tubular epithelium. CONCLUSIONS – combined administration in rats of thyroxin and propylthiouracilum has weakly expressed beneficial effect by limiting the development of structural damages to the renal parenchyma and clot formation. 

scholarly journals Functional state of the adrenal glands with combination of their morphological reorganization at different stages of a posthypothermic period

2019 ◽  
Vol 23 (1) ◽  
pp. 41-45
Author(s):  
T.V. Knyazevich-Chorna ◽  
I.O. Mikhailyuk ◽  
A.N. Rudyak ◽  
N.R. Tarasevych
Keyword(s):  
Functional State ◽  
Adrenal Glands ◽  
Arithmetic Mean ◽  
Deep Hypothermia ◽  
The Body ◽  
Male Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Submicroscopic Structure ◽  
Characteristic Structure ◽  
The Mean

The problem of human adaptation to extreme environmental factors, in particular to the cold, which is one of the most common, is relevant from general biological and medical points of view. In this case, morphological reorganization takes place in various organs and tissues, and sometimes the changes become irreversible. That’s why from a scientific and practical point of view it is important to assess the role of the cold factor, the influence of which is experienced by most living beings, in particular on the adrenal glands, which are among the first to react to changes in the internal environment of the body. The purpose of the study is to follow the dependence and to study the morphological and functional state of the adrenal glands at various stages after the action of general deep hypothermia. The morphological and functional state of the adrenal glands of white outbred adult male rats was studied using histological, electromicroscopic, enzyme-linked immunosorbent assay, fluorometric, and statistical methods of research in various periods after exposure to general deep hypothermia. The morphometric analysis of the thickness of the adrenal cortex zones, the diameter of the microvessels, the area of cells and their cores, both cortical and brain, was performed using the software VIAVISION Version 2. The average arithmetic mean (M) and the mean arithmetic mean (m) error using the software were determined. “Statistica-5,0”. The probability of the difference between the mean values and their errors was determined by the criterion of the Student. It has been established that at the height of the cold action, the levels of cortisol and adrenaline increase, along with hypertrophy of the cells of the fasciculate zone and the adrenal medulla. At the stages of the posthypothermic period, one more rise of corticosteroids is observed on the 7th day, while the concentration of catecholamines decreases. The hypertrophy of the adrenocorticocyte of the fasciculate zone persists until the 30th day, and the adrenocyte of the medulla lasts up to 7 days after the action of general deep hypothermia. On the 14th day, in the structural components of the hemomicrocirculatory bed, next to intracellular compensatory-adaptive phenomena, single microvessels with destructive signs of endothelial cells are noted. Until the 30th day, the vast majority of microvessels acquire the characteristic structure of the norm. These changes indicate a close relationship of the sympathoadrenal and hypothalamic-pituitary-adrenocortical systems. The results of the conducted research expand modern ideas about the features of angioarchitecture and the submicroscopic structure of the adrenal parenchyma, reveal the structural and functional bases of their restructuring after exposure to general deep hypothermia.

scholarly journals The influence of cycling on the functional state of young athletes 14-15 years old

2020 ◽  
pp. 148-152
Author(s):  
Maryna Prudnikova
Keyword(s):  
Heart Rate ◽  
Functional State ◽  
Vital Capacity ◽  
Physical Exertion ◽  
The Body ◽  
Primary Data ◽  
Mountain Bike ◽  
Young Athletes ◽  
Significant Difference ◽  
Respiratory Systems

At present, due to the constant growth of sports results and intensified competition at competitions of various levels in cycling (MTB), the problem of adaptation of the body to specific physical loads is becoming urgent As you know, the most violent processes in the body of a young man occur in the period from 13 to 15 years old, where all the functions and systems of a young body under the influence of physical exertion are of particular importance. Many works have shown that at a young age it is impossible to predict sports results in a chosen sport without taking into account functional characteristics. The article presents the indicators of the cardiovascular and respiratory systems of the body of young athletes 14-15 years old, who specialize in mountain bike. Under the influence of specific physical loads, changes in the functional state of young athletes of 14-15 years old in the basic, pre-competitive and restorative mesocycles were determined. The aim of the work was to determine the changes in the work of the cardiovascular and respiratory systems of the organism of young athletes 14-15 years old, specializing in mountain bike. A significant difference was found in the indicators of heart rate, maximum oxygen consumption, vital capacity of the lungs in the recovery and maintenance microcycles of the recovery mesocycle. The statistical difference was determined in terms of systolic and diastolic blood pressure (t=2.61; t=2.73; p<0.05), heart rate (t=2.51; p<0.05), vital capacity of the lungs (t=2.28; p<0.05) young athletes 14-15 years old in the recovery and maintenance microcycles of the recovery mesocycle relative to the primary data.

scholarly journals Peculiarities of structural reorganization of the thyroid gland vessels in dynamics after experimental thermal trauma

2018 ◽  
Vol 22 (4) ◽  
pp. 610-615
Author(s):  
V.G. Korytskyi
Keyword(s):  
Thyroid Gland ◽  
Structural Changes ◽  
Burn Injury ◽  
White Male ◽  
The Body ◽  
Male Rats ◽  
Time Interval ◽  
Thermal Trauma ◽  
Vascular Bed ◽  
Ketamine Anesthesia

Thermal trauma and burn disease, which develops from deep, large lesions, are accompanied by significant morphological and functional changes not only in the skin, but also in organs of all systems of the body, including thyroid gland. The purpose of the study was to establish a microscopic reorganization of the animal thyroid gland in dynamics after an experimental thermal trauma. Burns of the third degree were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97–100 °С. The affected area corresponded 18–20% of the surface of the rat’s body. Experimental study of structural changes in the vascular bed of the thyroid gland after severe thermal trauma was carried out on laboratory white male rats weighing 160–180 g. Euthanasia of rats was performed after ketamine anesthesia, by decapitation. Structural changes in the thyroid gland were studied after 1, 7, 14 and 21 days from the beginning of the experiment. Histologic sections of 5–6 µm thickness were stained with hematoxylin-eosin. For the study of the connective tissue condition and its main substance, silver impregnation was performed by using the Gordon-Sweet's method (to detect reticular and immature collagen fibers), staining with 3-colored MSB — selective coloring method for intravascular coagulation syndrome. It has been established that in the early stages after the thermal trauma (1–7 days of the experiment, the stage of shock and early toxemia) there are adaptive-compensatory changes and initial signs of destruction of the vascular bed structural components of the organ. In late terms (14–21 days of the experiment, the stages of late toxemia and septicotoxemia) significant destructive-degenerative changes of the thyroid gland arteries, veins and blood vessels of the hemocirculatory bed were detected. Therefore, a severe burn injury causes deep histological modification of the thyroid gland vessels, the degree of intensity of which depends on the time interval of the experiment, which adversely affects on the functioning of the organ and transvascular metabolic processes. In further researches it is planned to study the course of morphological changes of thyroid gland vessels during thermal trauma with combined application of corrective methods.  

Physiologically assessing the functional state of the body of doctors in children's medical organizations

2020 ◽  
Vol 60 (11) ◽  
pp. 775-777
Author(s):  
Anna V. Zayitceva ◽  
Khistina T. Oniani
Keyword(s):  
Cardiovascular System ◽  
Shift Work ◽  
Functional State ◽  
Night Shift ◽  
Circulatory System ◽  
Holter Monitoring ◽  
The Body ◽  
Pronounced Increase ◽  
Functional Capabilities ◽  
Shift Work Schedule

Introduction. Increased nervous and emotional loads in doctors of children's medical organizations are caused by frequent stressful effects, shift work mode. The study of the features of the formation of the functional state of the body of surgeons will make it possible to scientifically justify measures to prevent overstrain to preserve the health of employees. The aim of study is to research the functional state of the body of doctors of children's medical organizations with a shift work schedule to justify and develop measures for physiological and hygienic optimization of work. Materials and methods. Complex physiological and hygienic studies included occupational analysis of labor activity, physiological studies of the state of the Central nervous system (CNS) and the cardiovascular system with the study of the features of response to functional loads (ECG recording on a 12-channel electrocardiograph and continuously using Holter monitoring). Results. The study of the features and degree of adequacy of the cardiovascular system's response to dosed physical activity revealed the tension of the regulatory mechanisms of the circulatory system with a pronounced increase in heart activity in surgeons during night hours. Conclusions. Stable indicators of CNS functions (concentration of attention, etc.) were established during various shifts, due to the requirements of the work itself, adverse changes in the cardiovascular system, more pronounced during the night shift, deterioration of ECG changes to functional load, which indicates a decrease in the functional capabilities of the myocardium.

scholarly journals THE MORPHOMETRIC RESEARCH IN OF THE SPORT

2020 ◽  
Vol 19 (4) ◽  
pp. 65-71
Author(s):  
S. Karatieieva ◽  
O. Slobodian ◽  
H. Honchar ◽  
S. Penzay ◽  
A. Karatieieva
Keyword(s):  
Structural Changes ◽  
Practical Importance ◽  
Morphological Characteristics ◽  
Physical Exertion ◽  
Point Of View ◽  
The Body ◽  
Mass Composition ◽  
Sports Performance ◽  
Biometric Data ◽  
The Creation

The main problem in training athletes is adequate selection and sports affiliation. Solving the problems of selection involves the creation of a model of the athlete of this sport, a certain set of characteristics that determine athletic performance. This requires anthropometric assessment, morphometric and biometric data to track physical and physiological parameters, information to assess performance and recovery in sports, modification of training regimes to prevent injuries, provide guidance on regulating the use of technologies that used in professional sports, as well as to research and make recommendations for the proper collection, storage and exchange of the health information. One of the important problems of modern morphology is the study of the transformations that occur in the body under the influence of various factors. This problem has acquired particular importance in connection with the development of sports. High sports performance associated with significant loads on the athlete's body encourage scientists, doctors, coaches to search for the physiological reserves of the body and search for optimal modes during training. From this point of view, the study of the transformations that take place in individual organs, systems and in the body as a whole, under the influence of physical loads of varying intensity and nature, is relevant and is of great practical importance. Studies have shown changes in the basic physiological and biochemical parameters in athletes, such as adaptation of the muscular, cardiovascular, and respiratory systems, the nature of muscle energy supply, and tissue metabolism processes. There are also scientific studies that reflect structural changes that appear in the body under the influence of physical exertion. The level of results in modern sports is so great that in order to achieve them, athletes need to have appropriate morphological and functional data, as well as excellent physical and mental abilities. Therefore, the main problem of training athletes is adequate selection and sports orientation. Solving the selection problems involves the creation of a model of an athlete of a given specialization, that is, a certain set of features that determine sports performance. The set of features and the order in which they are listed is different for different sports. In the conditions of sports selection, such morphological characteristics as total body dimensions (length), body proportions, body mass composition are taken into account.

scholarly journals Effects of Regular Physical Activity on the Visual Analyzer and the General Condition of Children and Adolescents

2018 ◽  
Vol 15 (3) ◽  
pp. 303-308
Author(s):  
O. V. Yurova ◽  
D. V. Andzhelova ◽  
A. A. Chayka
Keyword(s):  
Physical Activity ◽  
Visual Acuity ◽  
Children And Adolescents ◽  
Functional State ◽  
Musculoskeletal System ◽  
Physical Exertion ◽  
The Body ◽  
Regular Physical Activity ◽  
Visual Analyzer ◽  
Excessive Body Weight

Purpose.to study the effect of physical exertion on the visual analyzer and the general functional state in children and adolescents who regularly engage in sports.Material and Methods.The study was conducted in a group of 600 children and adolescents aged from 8 to 17 years who were divided into two groups of 300 people: the I group — “athletes” (regularly engaged in sports), the second group — “schoolchildren” (those engaged in sports less than 3 hours per week). Each group was divided into two subgroups of 150 people — “emmetrops” and “myopes”. The parameters of visual acuity, axial eye length, indices of regional eye hemodynamics were evaluated.Results.After 12 months, the group “athletes” showed a statistically significant decrease in refraction on average by 0.14 ± 0.18 on the background of stable visual acuity. In the group “schoolchildren” there was a significant reduction in visual acuity without correction by 0.06 ± 0.19 (p = 0.004) and refractive indices by 0.35 ± 0.61 (p < 0.001) on the average. After 12 months, among the emmetrops, myopia was diagnosed in 6 % of cases in the “athletes” group and 10 % in the “schoolchildren” group. Among the “myopes” in both groups there was a significant increase in the body mass index and the number of persons with impaired functional state of the musculoskeletal system in the form of a violation of posture and shape of the foot in comparison with the “emmetrops”.Сonclusion.Regular physical activity can be considered as one of the factors preventing the development and progression of myopia, and functional disorders of the musculoskeletal system in the form of violation of posture and shape of the foot and excessive body weight — as possible risk factors for the development of myopia.

Sign in / Sign up

Export Citation Format

Share Document