scholarly journals Aktivitas Antibakteri Ekstrak dan Fraksi dari Ekstrak Metanol Daun Manggis (Garcinia mangostana) pada Bakteri Penyebab Ulkus Diabetik

2021 ◽  
Vol 7 (1) ◽  
pp. 31-38
Author(s):  
Vivi Rosalina ◽  
R Mahendra
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
The Body ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Diabetic Ulcer ◽  
Diabetic Ulcers ◽  
Concentration Series

Diabetes mellitus (DM) is a degenerative disease due to metabolic disorders with acute to chronic hyperglycemic conditions. The complication of this disease is peripheral neuropathy exacerbated by bacterial infection, causing ulcers (sores) on the feet, hands, knees, back, and other parts of the body. The mangosteen plant (Garcinia mangostana) is empirically believed to have antidiabetic and cure diabetic ulcers. This study aims to compare the antibacterial activity of extracts and fractions of methanol extract of mangosteen leaves at concentrations of 20%, 40%, 60%, 80%, and 100% against the bacteria causing diabetic ulcers in vitro. The research began with the maceration of mangosteen leaf powder extraction using methanol as a solvent. The fractions were obtained by the vacuum liquid chromatography (KCV) method. The bacteria that cause diabetic ulcers are obtained from ulcer specimens of diabetic ulcer patients. Antibacterial activity of extracts and fractions at 5 concentration series was tested using the disc diffusion method with 10% DMSO negative control and positive controls, namely Amoxicillin, Ampicillin, Ciprofloxacin, Cefotaxime, Gentamycin, Erythromycin, and Doxycycline. Antibacterial activity was expressed by the large area of the inhibition zone on the media inoculated with ulcer bacteria and incubated for 24 hours at 370 C. The results showed that there were 5 dominant bacteria found in the diabetic ulcer specimens, namely Escherichia spp, Klebsiella spp, Staphylococcus spp, salmonella spp, and Shigella spp. The results of the antibacterial sensitivity test showed that ciprofloxacin had the largest area of inhibition zone compared to Amoxicillin, Ampicillin, Cefotaxime, Gentamycin, Erythromycin, and Doxycycline so that Ciprofloxacin could be used as a positive control. The results of the antibacterial activity test for the methanol extract of mangosteen leaves with a concentration of 100% produced the largest inhibition zone, namely 27.10 mm ± 0.1 compared to methanol extract, fractions A, B, and C in all concentration series. However, the inhibition zone of the 100% concentration of mangosteen leaves methanol extract was smaller than the positive control of ciprofloxacin 5 μg / disk, which was 35.01 mm ± 0.1.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi

2020 ◽  
Vol 8 (3) ◽  
pp. 177
Author(s):  
Achmad Syarifudin Noor ◽  
Bawon Triatmoko ◽  
Nuri Nuri
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction ◽  
Zone Diameter

The leaves of kenikir are known to have many benefits, one of which can be used as medicine. Kenikir leaves are reported to have antibacterial activity against the bacteria Salmonella typhi (S.typhi). S. typhi is a bacterium that causes typhoid fever. Typhoid fever is still an endemic disease in Asian countries, especially Indonesia. Typhoid fever became the third largest disease in Indonesia in 2010. The study related to the antibacterial activity of kenikir leaf fraction to S. typhi has never been reported. Therefore, it is necessary to conduct research related to the antibacterial activity of extracts and leaf fractions of kenikir to compare their potential against S. typhi. The study was conducted using the disk diffusion method using 5 series of concentrations namely 5%, 10%, 15%, 20%, and 30%. Tests were carried out on methanol extract, hexane, ethyl acetate, and methanol-water fraction. The results showed that highest inhibition zone diameter is 7,75 mm which is reached by methanol extract. The order of the highest inhibiting zone diameter are extract, hexane, ethyl acetate, and methanol-water fraction.

scholarly journals CRUDE EXTRACTS OF MAGNOLIA ALBA FLOWERS USING N-HEXANE AND METHANOL SOLVENTS AND THEIR ANTI-ACNE POTENTIAL

2021 ◽  
Vol 10 (6) ◽  
pp. 3789-3793
Author(s):  
Sofia Munira
Keyword(s):  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Positive Control ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Bacterial Inhibition

This study aimed to test the antibacterial activity of n-hexane and methanol extracts of white cempaka flowers (Magnolia alba) against Propionibacterium acnes bacteria. The extract was obtained by using the maceration method for 72 hours. Each extract was tested for its antibacterial properties using the disc diffusion method with the variant concentration of the extract used was 0.05%; 2%; 4%; 6%; and 8%. The results showed that the methanol extract had a higher inhibition zone than the n-hexane extract. In n-hexane extract, the lowest concentration of 0.05% showed an inhibition zone of 6.6 mm and the highest concentration of 8% had an inhibition zone of 9.6 mm. Whereas in methanol extract, the inhibition zone obtained was 7.3 mm at a low concentration of 0.05%, but at the highest concentration of 8% the inhibition zone obtained was 11.6 mm. The effect of extract concentration on the diameter of the inhibition zone formed is very significant (directly proportional), where the higher the concentration used, the larger the diameter of the inhibition zone produced. Antibacterial testing for both extracts showed a weak (< 10 mm) to moderate (> 10 mm) bacterial inhibition, which was similar with the inhibition zone obtained from the positive control (11-11.3 mm; moderate bacterial inhibition).

scholarly journals The potency of ethanolic extract from corn silk as natural antibiotics for acne-related bacteria: A preliminary study

2022 ◽  
Vol 21 (1) ◽  
pp. 84-89
Author(s):  
Fitri A Nurani ◽  
Nadia RS Rejeki ◽  
Tanti Setyoputri ◽  
Putri K Wardani ◽  
Fatkhan B Ridwan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Acne Vulgaris ◽  
Medical Science ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Traditional Medicines ◽  
Corn Silk

Introduction: Corn silk or known as herb name stigma maydis is an important medicinal botanical in many traditional medicines worldwide, including jamu, an Indonesia traditional medicine. The exploration of corn silk to treat acne vulgaris is still lacking, therefore the current research was conducted to analyze the activity of ethanolic extract from corn silk (EECS) against 3 acne-related bacteria, Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus. Materials and Methods: The antibacterial activity of EECS at concentration range of 10 to 100% v/v was evaluated using the disk diffusion method. As comparation, distilled water was used as a solvent control, while 1% clindamycin was used as a positive control. Results: Shinoda’s test showed that flavonoid was detected in the EECS. The higher concentration of EECS exhibited higher diameter of inhibition zone indicating higher antibacterial activity on P. acnes, while the antibacterial activity of S. epidermidis was not increased at similar concentrations of EECS. The antibacterial activity of EECS against S. aureus decreased at the higher EECS concentration (>70%). Conclusion: Taken together, EECS is a potential as a bioactive source to inhibit the growth of acne-related bacteria P. acne, S. epidermidis and S. aureus. Further investigation is needed to explore the corn silk or stigma maydis as a medicinal botanical in jamu targeted to treat acne vulgaris. Bangladesh Journal of Medical Science Vol. 21(1) 2022 Page : 84-89

scholarly journals GEL ANTI JERAWAT EKSTRAK DAUN BUTA-BUTA (Excoecaria agallocha L.) DAN PENGUJIAN ANTIBAKTERI Staphylococcus epidermidis

2015 ◽  
Vol 1 (2) ◽  
pp. 65-72
Author(s):  
Ika Olivia Borman ◽  
Yusriadi Yusriadi ◽  
Evi Sulastri
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Diffusion Method ◽  
Activity Data ◽  
Excoecaria Agallocha ◽  
Significant Difference ◽  
The Stability

Buta-Buta (Excoecaria agallocha L.) leaf is a type of mangrove plants that has antibacterial activity againtsbacteriacausing acne, Staphylococcus epidermidis,. This study aims to formulate buta-buta leaf extract with the concentration of 12.5 % into anti-acne gel using two gels, namely HPMC 8% (Formula 1) and Carbopol 940 2%(Formula 2). Antibacterial activity test was carried out by agar diffusion method using well. The stability and antibacterial activity data during 28 days were analyzed statistically using unpaired t-student with a degree of confidence of 95% (α = 0.05). The evaluation results of anti-acne gel form of buta-buta leaf extract in Formula 2 showedbetter characteristics than Formula 1, which include organoleptic examination, homogenity, pH and viscosity during 28 days. The antibacterial test showed the diameter of inhibition zone of Formula 2 is 11.03 mm ±0,01. The statistic analysis showed a significant difference between Formula 2 with a positive control (klindamisin gel).

scholarly journals Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 34-38
Author(s):  
Nurul Cholifah ◽  
Ahmad Ridhay ◽  
Pasjan Satrimafitrah ◽  
Ruslan ◽  
Hardi Ys
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Methanol Extract ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Bark Extract ◽  
Moringa Oleifera Lam

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli

scholarly journals FORMULASI SEDIAAN GEL PEEL-OFF EKSTRAK BUAH LIMPASU (Baccaurea lanceolate (Miq) Mull.Arg.) SEBAGAI ANTIBAKTERI

2021 ◽  
Vol 6 (2) ◽  
pp. 361-367
Author(s):  
Deni Setiawan ◽  
◽  
Nur Mahdi ◽  
Muhammad Riki Shindi Praristiya
Keyword(s):  
Antibacterial Activity ◽  
Protective Layer ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
The Body ◽  
Premature Aging ◽  
Fruit Peel ◽  
Fruit Extract ◽  
Preliminary Identification

Skin is a protective layer of the body as well as an aesthetic factor to protect from exposure foreign substances and sunlight. Some problems that may arise on the skin include premature aging and acne. The use of natural ingredients as cosmetics is increasing, one of which is the limpasu fruit peel-off gel mask. This study aims to determine the antibacterial activity of the ethanol extract of limpasu fruit extract. The formulation was made with three concentrations of ethanol extract of limpasu fruit (Baccaurea lanceolate) 4, 6 and 8 grams. The formula was tested on E.coli. The yield of limpasu fruit extract was 30.49 grams or 7.81%. Results of preliminary identification of ethanol extract containing saponins and flavonoids. The results of the antibacterial test formulas 1 to 3 had inhibition zones 12, 53; 7.94 and 9.97 mm with a positive control of tetracycline which had an average inhibition zone of 28.87 mm. The peel-off gel formulation had antibacterial activity because the average inhibition zone was more than 6 mm.

scholarly journals ANTIBACTERIAL ACTIVITY of COMBINATION COLD PLASMA and PARIJOTO (Medinilla speciosa) against Staphylococcus aureus and Pseudomonas aeruginosa on DIABETIC ULCER

el–Hayah ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 133-138
Author(s):  
Fajar Farra Anastasia ◽  
Itsna Risqii Aziz ◽  
Vanny Oktaviola ◽  
Arya Iswara
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Cold Plasma ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Diabetic Ulcer ◽  
Inhibition Zones ◽  
Dominant Bacteria

This study aims to determine the potential of cold plasma (cold plasma) as an antibacterial if combined with Parijoto (Medinilla speciosa) extract against the dominant bacteria in diabetic ulcer infection, namely Gram-positive Staphylococcus aureus and Gram-negative bacteria Pseudomonas aeruginosa with a concentration of 25%, 50 %, 75%, and 100%. This study was an experimental test in vitro using the well diffusion method. The well diffusion method uses an MHA given a 5mm diameter and inserted a 100uL sample then incubates 35 ± 2 ° C for 16-18 hours. The results showed antibacterial activity from Cold Plasma with a 10 mm shot distance for 3 minutes with inhibitory zones against S. Aureus 3 mm and against P. aeruginosa 3.35 mm, a combination of Cold Plasma antibacterial activity with parijoto extract with a zone of inhibition of S. aureus 11.7 mm (25%), 14.1 (50%), 16.5 (75%), 17.1 (100%) and against P. aeruginosa 7.7 mm (25%), 9.3 mm (50%), 10.1 mm (75%), 11.7 (100%). the results of the Cold Plasma inhibition zone and Cold Plasma combination with parijoto extract 100% concentration of S. aureus were greater than the inhibition zone for P. aeruginosa. Cold Plasma combination inhibition zones with starch extracts against S. aureus compared to Vancomicin (VA) antibiotics with a 19.9 mm inhibition zone included in the intermediate category, Cold Plasma combination inhibition zone with parijoto juice against P. aeruginosa bacteria compared with Meropenem antibiotics ( MRP) with a 9.7mm inhibition zone included in the sensitive category

scholarly journals Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 201 ◽  
Author(s):  
Suryadi Budi Utomo ◽  
Mita Fujiyanti ◽  
Warih Puji Lestari ◽  
Sri Mulyani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Hexadecyltrimethylammonium Bromide ◽  
E Coli ◽  
Escherichia Coli Bacteria

<p>This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the <em>S.</em><em> </em><em>aureus</em> bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against <em>E. coli</em>.</p>

scholarly journals Pengujian Aktivitas Antibakteri Hidrogel Ekstrak Etanol Daun Tagetes erecta L

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
Hosea Jaya Edy ◽  
Marchaban Marchaban ◽  
Subagus Wahyuono ◽  
Agung Endro Nugroho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Tagetes Erecta L

Kandungan kimia flavonoid dan fenolik dalam ekstrak daun T.erecta diketahui memiliki aktivitas antibakteri yang baik. Pengunaan ekstrak daun T.erecta sebagai zat aktif sediaan hidrogel akan sangat mempermudah pemanfaatan dalam proses terapi oleh masyarakat. Tujuan dalam penelitian ini adalah melihat kemampuan aktivitas antibakteri sediaan hidrogel dengan zat aktif ekstrak daun T.erecta. Dalam penelitian ini digunakan 6 variasi konsentrasi ekstrak yang terkandung pada sediaan hidrogel yaitu 1.5; 2.0; 2.5; 3.0; 3.5; dan 4.0 %. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas antibakteri terbesar ditunjukkan oleh sediaan hidrogel dengan konsentrasi ekstrak daun T.erecta 2.5%. Diameter zona hambat yang terbentuk terhadap pertumbuhan E.coli adalah 15.33 mm sedangkan diameter zona hambat terhadap S.aureus adalah 17.87 mm. Untuk kontrol positif digunakan bioplacenton® dan untuk kontrol negatif digunakan basis hidrogel tanpa kandungan ekstrak T.erectaFlavonoid and phenolic on ethanolic extract of T.erecta L has been antibacterial activity. The aim of this research was to determinate of antibacterial from hydrogel with ethanolic extract of T.erecta L. This research was begun making hydrogels with six variations of ethanolic extract of T.erecta L were 1.5; ; 2.0; 2.5; 3.0; 3.5; and 4.0 %.  Antibacterial activity used hole diffusion method against Staphylococcus aureus and Escherichia coli. Hydrogel containing 2.5 % ethanolic extract of T.erecta L was shown the highest antibacterial activity. The diameters of the inhibition zone against E.coli was 15.33 mm and against S.aureus was 17.87 mm. Positive control used bioplacenton® and negative control was hydrogel without ethanolic extract of T.erecta content

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