Comparison between Ocimum Sanctum Hepatoprotector Extract and Curcuma Xanthorrhiza on the Histological Structure of Aspartame-Induced Wistar Rats

The use of aspartame is still controversial, because there are studies stating that aspartame is safe to use, and there are studies suggesting aspartame has the potential to damage the liver, but aspartame has been approved by the FDA and BPOM in Indonesia with a daily dose of 50 mg / kg / day, the level of public knowledge we are still low to allow this dose to be overtaken, coupled with the presence of several food products that do not include the content of aspartame, basil leaves have been known to have hepatoprotector effect, but the dosage is still varied, and no researchers have compared curcuma Xanthoriza which is herbal medicine that has been quite well accepted in the community, Objective: to compare the hepatoprotector effect of basil leaf extract with xanthoriza curkuma, Method: Laboratory experimental study with posttest only with control group design. Wistar rats were divided into 5 groups and treated for 30 days. This study analyzed the histopathology of liver using paraffin histotechnics blocks, with HE staining and light microscopy. Analysis of degeneration degree using Kruskal-walis analysis post Hoc Mann-Whitney Results showed an increase in the degree of degeneration in the aspartame group at a dose of 100 mg / kgbb / day (p <0.05) compared to the normal group and treatment. Use of aspartame past the ADI dose damaged the liver, kurkuma and basil leaf extract at a dose of 300 mg / kg has the same protective effect on aspartame-induced rat liver, conclusions: aspartame is toxic for the liver, the use of basil leaf extract at a dose of 300 mg / kg / day or kurkuma xhantoriza extract can reduce the toxicity of aspartame.

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THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

Dentino : Jurnal Kedokteran Gigi ◽

10.20527/dentino.v6i2.11999 ◽

2021 ◽

Vol 6 (2) ◽

pp. 159

Author(s):

Nabilah Nabilah ◽

Irham Taufiqurrahman ◽

Nolista Indah Rasyid

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Total Sample ◽

Control Group ◽

Control Group Design ◽

Gel Effect ◽

Index Measurement ◽

Male Wistar Rats ◽

Collagen Density ◽

Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

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THE EFFECT OF RAMANIA LEAF (Bouea macrophylla Griff) EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN INCISIONAL WOUND OF MALE WISTAR RATS

Dentino : Jurnal Kedokteran Gigi ◽

10.20527/dentino.v6i1.10648 ◽

2021 ◽

Vol 6 (1) ◽

pp. 96

Author(s):

Dinda Rizkia ◽

Irham Taufiqurrahman ◽

Renie Kumala Dewi

Keyword(s):

Wistar Rats ◽

Leaf Extract ◽

Collagen Fibers ◽

The Body ◽

Control Group ◽

Control Group Design ◽

Treatment Groups ◽

Significant Difference ◽

Male Wistar Rats ◽

Post Hoc

ABSTRACTBackground: Ramania leaf (Bouea macrophylla Griff) extract gel has secondary metabolites in the form of flavonoids, steroids, phenols and terpenoids which have a role as antioxidant. They will protect the body from excessive production of reactive oxygen species (ROS) by increasing endogenous antioxidants SOD, CAT and GPX, so that wound healing will not be inhibited and the process of collagen synthesis can run smoothly. Objective: To analyze the effect of ramania leaf extract gel that was applied topically with 5%, 10% and 15% concentration on collagen fibers density in incisional wound of male Wistar rats (Rattus norvegicus) on the 7th and the 14th day. Method: This research is a pure experimental study with a posttest-only control group design, using 24 rats which were divided into 4 groups: the treatment groups given ramania leaf extract gel of 5%, 10%, 15%, and the control group given placebo gel. The application of the extract gel was done once within 24 hours. The collagen level was measured with a spectrophotometer on the 7th and the 14th day. Results: Two-Way ANOVA test results on the 7th and the 14th day of each group showed a significant difference with p=0.000 (p<0.05). The Bonferroni Post-hoc Test showed a significant difference with p<0.05 between the placebo gel group and the groups of ramania leaf extract gel of 5%, 10%, 15% on the 7th and the 14th day. Conclusion: There is an effect of ramania leaf extract gel on collagen fibers density with the most effective concentration of 15%.Keywords: Antioxidant, , Collagen, Ramania Leaf Extract Gel

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ANTIBACTERIAL EFFECTIVITY OF KASTURI LEAF EXTRACT (Mangifera casturi) AGAINST THE GROWTH OF Streptococcus sanguinis BACTERIA

Dentino : Jurnal Kedokteran Gigi ◽

10.20527/dentino.v5i1.8118 ◽

2020 ◽

Vol 5 (1) ◽

pp. 33

Author(s):

Dita Puspita Sari ◽

Didit Aspriyanto ◽

Irham Taufiqurrahman

Keyword(s):

Leaf Extract ◽

Periodontal Diseases ◽

Tooth Surface ◽

Control Group ◽

Dilution Method ◽

Control Group Design ◽

Streptococcus Sanguinis ◽

Significant Difference ◽

Post Hoc ◽

One Way Anova

Background: Caries is a disease that occurs because of the fermentation carbohydrates process by microorganisms in the oral cavity. One of the bacteria that causes caries is Streptococcus sanguinis. These bacteria will colonize on the tooth surface, then form dental plaques and contribute to the causes of caries and other periodontal diseases. Kasturi leaf extract (Mangifera casturi) has various compounds such as tannins, terpenoids, alkaloids, and flavonoids that have antimicrobial substances. Purpose: The aim of this study was to determine antibacterial effectivity of kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis bacteria. Method: This research was an experimental method laboratory (true experimental), with a randomized pre test and post test with control group design using 5 treatments: kasturi leaf extract (concentration: 20 mg/ml, 25 mg/ml, and 30 mg/ml); and two groups of control: positive control and negative control. Each treatment was repeated 5 times. Antibacterial activity testing used a liquid dilution method. Measurement of minimum inhibitory concentration (MIC) used a Uv-Vis Spectrophotometer and measurement of the minimum bactericidal concentration (MBC) used a colony counter. The MIC data were analyzed using One Way Anova and continued with the Dunnet Post Hoc test. MBC data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney Post Hoc test. Result: One-Way Anova test showed that MIC had a significant difference, and the Kruskal-Wallis test showed that MBC also had significant differences. MIC was obtained at the concentration of 20 mg/ml and MBC was obtained at the concentration of 30 mg / ml. Conclusion: There is antibacterial effectiveness in kasturi leaf extract (Mangifera casturi) against the growth of Streptococcus sanguinis.

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THE EFFECT OF SEMBUNG LEAF EXTRACT (BLUMEA BALSAMIFERA) ON THE NUMBER AND DIAMETER OF RATS LEYDIG CELLS INDUCED BY HIGH-FAT DIET

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no1.049 ◽

2021 ◽

Vol 21 (no 1) ◽

Author(s):

Gede Widhiantara ◽

Anak Agung Ayu Putri Permatasari ◽

Putu Angga Wiradana

Keyword(s):

Treatment Group ◽

Leydig Cells ◽

Wistar Rats ◽

Leaf Extract ◽

Control Group ◽

Control Group Design ◽

Core Diameter ◽

Post Test ◽

Male Wistar Rats ◽

The Mean

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.

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Effectiveness of Spirulina platensis Extract on Wound Area and TNF-a Levels on Blood: Experimental Studies In Wistar Rats Made Artificially by Vulnus Scissum and Infected by Staphylococcus aureus

Indonesian Journal of Environmental Management and Sustainability ◽

10.26554/ijems.2020.4.2.55-58 ◽

2020 ◽

Vol 4 (2) ◽

Author(s):

Olvaria Misfa ◽

Renni Yuniarti ◽

Yan Wisnu Prajoko

Keyword(s):

Wistar Rats ◽

Experimental Studies ◽

Serum Levels ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Wound Area ◽

Post Hoc

This research uses randomizedpost-test only control group design. Thirty-two (32) male Wistar rats with incised skin and infected with S. aureusdivided into 4 groups, namely the group given S.platensisextract at a dose of 500 mg / kgBB / day (X1) and a dose of 750 mg / kgBB / day (X2 ), the negative control group was given saline solution (C1), and the positive control group was given amoxicillin 150 mg / kg orally(C2). Wound area measurements were taken on day 14 and serum TNF-? levels were examined on day 14 using the ELISA method. Data analysis was performed with one way ANOVAtestand continued with Post Hoc Test LSD.The results showed mean size of wound closure on the 14th day in groups X1, X2, C1 and C2 is 8095.74; 6270.98;15502.69; 11475.20micrometer with p <0.001. The mean TNF-? serum levels in the 14th day of X1, X2, C1, and C2 groups were 270.75; 222.83; 1730.33; 385.75pg / ml with p <0.001. Post Hoc Test of wound area showed significant differences between groups. Post Hoc Test TNF-? levels showed significant differences between treatment groups X1 and X2 with group C1.Spirulina platensisextract 500mg / kgBB / day and 750 mg /kgBB/ day have the smallest wound area significantly and reduce TNF-? levels on blood.

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EFEK ANTI HISTAMIN EKSTRAK DAUN JAMBU BIJI PADA TIKUS PUTIH YANG DIINDUKSI OVALBUMIN DINILAI DARI JUMLAH EOSINOFIL JARINGAN KULIT

Smart Medical Journal ◽

10.13057/smj.v2i2.33074 ◽

2020 ◽

Vol 2 (2) ◽

pp. 66

Author(s):

RENDRA RISTIAN WIBOWO ◽

RATIH DEWI YUDHANI ◽

NOVAN ADI SETYAWAN

Keyword(s):

Leaf Extract ◽

Allergic Diseases ◽

Psidium Guajava ◽

Skin Tissue ◽

Control Group ◽

Control Group Design ◽

White Rats ◽

Quasi Experimental ◽

Guava Leaf ◽

Post Hoc

ABSTRAKPendahuluan: Prevalensi dan keparahan penyakit alergi terus meningkat secara global, terutama di negara dengan pendapatan rendah sampai menengah. Terapi alergi saat ini seperti kortikosteroid banyak memberikan efek samping dalam penggunaan jangka panjang. Ekstrak daun jambu biji (Psidium guajava) berpotensi sebagai antihistamin yang dapat menjadi terapi alternatif penyakit alergi. Penelitian ini bertujuan untuk mengetahui efek antihistamin ekstrak daun jambu biji (Psidium guajava) pada tikus putih (Rattus novergicus) yang diinduksi ovalbumin dinilai dari jumlah eosinofil jaringan kulit. Metode: Penelitian ini merupakan penelitian quasi experimental design dengan rancangan post test only with control group design. Subjek penelitian tikus putih jantan, umur 3-4 bulan, dan berat badan 200-300 gram. Sampel 24 ekor tikus putih dibagi secara random menjadi enam kelompok. Kelompok kontrol normal (K) tanpa perlakuan, kontrol negatif (KN) hanya diinduksi ovalbumin, kontrol positif (KP) diberikan metilprednisolon, dan kelompok perlakuan (KP1, KP2, KP3) diberikan ekstrak daun jambu biji dengan dosis berikut 27 mg/200 gramBB, 54 mg/200 gramBB, dan 108 mg/200 gramBB. Efek antihistamin dinilai melalui gambaran histopatologi jaringan kulit berupa rata-rata jumlah infiltrasi eosinofil dengan menggunakan mikroskop cahaya. Data dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan uji Post Hoc Mann Whitney (α= 0,05).Hasil: Uji Kruskal Wallis menunjukkan perbedaan signifikan di antara enam kelompok perlakuan (p=0,00). Uji Post Hoc Mann Whitney menunjukkan perbedaan signifikan di antara semua pasangan kelompok (p=<0,05). KP3 menunjukkan rerata jumlah eosinofil lebih rendah daripada KP dan signifikan (p=0,021). Kesimpulan: Ekstrak daun jambu biji (Psidium guajava) memiliki efek antihistamin dengan menurunkan infiltrasi eosinofil pada jaringan kulit tikus putih (Rattus norvegicus) yang dinduksi ovalbumin.Kata Kunci: Ekstrak daun jambu biji, eosinofil, tikus putih, histopatologi kulit. ABSTRACTIntroduction: The prevalence and severity of allergic diseases continues to increase globally, especially in countries with low to middle income. Current allergic therapies such as corticosteroids have many side effects in long-term use. Guava leaf extract (Psidium guajava) has the potential as an antihistamine which can be an alternative therapy for allergic diseases. This study aims to determine the effect of antihistamine extract of guava leaves (Psidium guajava) on white rats (Rattus novergicus) induced by ovalbumin assessed from the number of eosinophils of skin tissue. Methods: This research was a quasi experimental design with a post test only design with control group design. The research subjects were male white rats, aged 3-4 months, and weighing 200-300 grams. Samples of 24 white rats were randomly divided into six groups. Normal (K) control group without treatment, negative control (KN) was only induced by ovalbumin, positive control (KP) was given methylprednisolone, and treatment groups (KP1, KP2, KP3) were given guava leaf extract with the following dosage 27 mg / 200 gram BW, 54 mg / 200 gram BW, and 108 mg / 200 gram BW. The effect of antihistamines was assessed through histopathology of skin tissue in the form of an average number of eosinophil infiltrations using a light microscope. Data were analyzed using the Kruskal Wallis test and followed by the Mann Whitney Post Hoc test (α = 0.05).Result: The Kruskal Wallis test showed significant differences between the six treatment groups (p = 0.00). The Mann Whitney Post Hoc test showed significant differences between all group pairs (p = <0.05). KP3 shows the average number of eosinophils lower than KP and significant (p = 0.021). Conclusion: Guava leaf extract (Psidium guajava) has an antihistamine effect by reducing eosinophil infiltration in ovalbumin-induced white rat (Rattus norvegicus) skin tissue.Keywords: Guava leaf extract; eosinophil; skin histopathology

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Pemberian Ekstrak Daun Cincau (Mesona palustris BL) Meningkatkan Kadar Superoksida Dismutase (SOD) Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

JURNAL BIOMEDIK (JBM) ◽

10.35790/jbm.10.1.2018.18995 ◽

2018 ◽

Vol 10 (1) ◽

Cited By ~ 1

Author(s):

Karnia Tarnajaya ◽

Alex Pangkahila ◽

Wimpie Pangkahila ◽

Ferbian M. Siswanto

Keyword(s):

Physical Training ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Leaf Extract ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Male Wistar Rats ◽

The Mean

Abstract: This study was aimed to prove that administration of black cincau (Mesona palustris BL) leaf extract increased superoxide dismutase (SOD) levels of overtraining-induced male Wistar rats (Rattus norvegicus). This was a true experimental study using the randomized pretest-posttest control group design. Subjects were 16 male rats (Rattus norvegicus), 6-month old, Wistar strain, weighing 180-200 g, SOD level <39.3 U/mL (normal SOD level), divided into two groups: the control group (P0) and the treatment group (P1). The P0 group was given excessive physical training and aquadest for 14 days, while the P1 group was given excessive physical training and black cincau leaf extract of 54 mg/200 g BW daily for 14 days. The results showed that the mean SOD level before treatment (pretest) in the P0 group was 30.63±2.26 U/mL while in the P1 group was 31.31±2.52 U/mL (P >0.05). After 14-day treatment (posttest), the mean SOD level in the P0 group was 29.36±2.83 U/mL while in the P1 group was 41.31±2.35 U/mL (P <0.01). Further analysis showed that there was no change of SOD levels in the P0 group (P > 0.05), but in the P1 group there was a significant increase of SOD levels from 31.31±2.52U/mL to 41.31±2.35U/mL (P <0.01). Conclusion: Administration of black cincau leaf extract of 54 mg per 200 g of body weight increased SOD level of overtraining-induced male Wistar rats (Rattus norvegicus).Keywords: black cincau leaves, SOD, excessive physical activityAbstrak: Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun cincau (Mesona palustris BL) dapat meningkatkan kadar superoksida dismutase (SOD) tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih. Jenis penelitian ialah eksperimental murni menggunakan randomized pretest-posttest control group design. Subjek penelitian ialah 16 ekor tikus putih jantan (Rattus norvegicus) galur Wistar berumur 6 bulan, sehat, berat badan 180-200 gr, dan kadar SOD <39,3 U/mL (kadar SOD normal), yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (P0) dan kelompok perlakuan (P1), masing-masing berjumlah 8 ekor tikus. Kelompok P0 diberikan pelatihan fisik berlebih dan akuades secara sonde selama 14 hari, dan kelompok P1 diberikan pelatihan fisik berlebih dan ekstrak daun cincau dosis 54 mg/200 gr BB tikus selama 14 hari. Hasil penelitian menunjukkan bahwa rerata kadar SOD sebelum perlakuan (pretest) pada kelompok P0 ialah 30,63±2,26 U/mL, sedangkan pada kelompok P1 ialah 31,31±2,52 U/mL (P >0,05). Setelah perlakuan selama 14 hari (posttest), rerata kadar SOD pada kelompok P0 ialah 29,36±2,83 U/mL, dan pada kelompok P1 ialah 41,31±2,35 U/mL (P <0,01). Hasil analisis efek perlakuan menunjukkan bahwa pada kelompok P0 tidak terjadi perubahan kadar SOD (P >0,05), namun pada kelompok P1 terjadi peningkatan bermakna kadar SOD dari 31,31±2,52 U/mL menjadi 41,31±2,35U/mL (P <0,01). Simpulan: Ekstrak daun cincau dosis 54 mg/200 gr BB tikus dapat meningkatkan kadar SOD tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih.Kata kunci: daun cincau, SOD, aktivitas fisik berlebih

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Particular Matter of Motor Vehicle Exposure and High-Fat Diet Effects on Kidney Histopathology, Creatinine, and Malondialdehyde (MDA) Levels in Wistar Rats

Indonesian Journal of Environmental Management and Sustainability ◽

10.26554/ijems.2021.5.3.124-128 ◽

2021 ◽

Vol 5 (3) ◽

pp. 124-128

Author(s):

Rizka Veni ◽

Awal Prasetyo ◽

Muflihatul Muniroh

Keyword(s):

High Fat Diet ◽

Wistar Rats ◽

Motor Vehicle ◽

Histopathological Change ◽

Normal Diet ◽

Control Group ◽

High Fat ◽

Control Group Design ◽

Treatment Groups ◽

Significant Difference

This study aims to analyze the effect of combination of motor vehicle particular matter exposure and high-fat diet in kidney histopathology, creatinine levels, and MDA levels in Wistar rats. This study used a posttest-only control group design. Eighteen healthy male Wistar rats were divided into three groups. The intervention groups received motor vehicle fume exposure for 100 s with normal diet (X1) or high-fat diet (X2), and the control group received no exposure (C). Data analysis was processed with a SPSS 25.0 computer program by using the one-way ANOVA test followed by post hoc LSD. The degree of kidney histopathological damage showed significant differences between the X1 and X2 groups when compared with the control group (p < 0.05). The results of the creatinine level examination found a significant difference between the X2 and C groups (p < 0.05) and the treatment groups X1 and X2 (p < 0.05). The results of kidney MDA level examination showed a significant difference between the treatment groups (X1 and X2) and the control group (p < 0.05). The combination of particular matter of motor vehicle fumes exposure and high-fat diet could induce kidney damage through histopathological change and increased creatinine levels and kidney MDA levels in Wistar rats.

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

ODONTO Dental Journal ◽

10.30659/odj.1.1.25-28 ◽

2015 ◽

Vol 1 (1) ◽

pp. 25

Author(s):

Laila Fitrotuz Zahroh ◽

Rahmawati Sri Praptiningsih ◽

Moh. Baehaqi

Keyword(s):

Oral Mucosa ◽

Wistar Rats ◽

Healing Process ◽

Research Result ◽

Aloe Vera ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

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