scholarly journals Enzyme-Treated Soybean Meal Replacing Extruded Full-Fat Soybean Affects Nitrogen Digestibility, Cecal Fermentation Characteristics and Bacterial Community of Newly Weaned Piglets

2021 ◽  
Vol 8 ◽  
Author(s):  
Hao Li ◽  
Jie Yin ◽  
Xi He ◽  
Zhiqing Li ◽  
Bie Tan ◽  
...  

The study investigated the impact of soybean protein from different processing on the performance, dietary nitrogen digestibility, cecal fermentation characteristics, and bacterial community in newly weaned piglets. The piglets were allocated to two dietary treatment and fed with the extruded full-fat soybean diet (EFS group) and enzyme-treated soybean meal diet (ESBM group), respectively. The piglets in ESBM group showed greater nitrogen digestibility and feed efficiency, and lower diarrhea rate in comparison to piglets in EFS group (P < 0.05). Cecal samples from piglets in ESBM group contained greater concentration of acetate, propionate and total SCFAs (P < 0.05), and lower contents of isobutyrate, isovalerate, total BCFAs, NH3-N and putrescine (P < 0.05) than cecal samples from piglets in the EFS group. The cecal samples from piglets in ESBM group contained greater abundances of g_Blautia, g_Coprococcus_3, g_Fusicatenibacter, and g_Bifidobacterium than the cecal sample from piglets in the EFS group, which could promote to protect intestinal health. In summary, enzyme-treated soybean meal may enhance the growth performance of weaned piglets via increasing the dietary nitrogen digestibility, preventing protein fermentation in the hindgut, which shed light on the mechanism in regulating gut health of dietary protein.

Author(s):  
Anca GHEORGHE ◽  
Mihaela HĂBEANU ◽  
Nicoleta Aurelia LEFTER ◽  
Daniela Mihaela GRIGORE

The effects of dietary extruded linseed (ELS):walnut meal (WM) mixture (8:1) on performance and plasma protein profile in weaned piglets was evaluated for 21 d. Topigs piglets (n=40; BW=8.02±0.82 kg), age 30±3 days, were allotted into 2 groups and fed 2 diets: control [C, based on corn-triticale-soybean meal (SBM)] and experimental (ELS:WM, where the ELS:WM mixture (8:1) partially replace SBM). Blood samples were collected at 7d and 21d after weaning. The plasma protein profile (total protein, total bilirubin, albumin, creatinine, uric acid, urea nitrogen-BUN) were determined by a chemistry analyser. Dietary ELS:WM mixture improve the BW (P=0.047) and ADG (P=0.036) of piglets at 21d after weaning vs C, whereas ADFI and F:G ratio were increase (P>0.05). The plasma protein profile of piglets fed dietary mixture was not affected at 7d and 21d after weaning, except plasma BUN concentration that was decrease (P=0.027) at 21d after weaning. Lower BUN concentration indicated higher availability of dietary nitrogen reflected in a higher deposition of protein. We concluded that dietary ELS:WM mixture improve piglets performance and positively afect plasma protein profile, especially BUN in weaning period.


2020 ◽  
Author(s):  
Wenxiu Zhang ◽  
Chengling Bao ◽  
Jian Wang ◽  
Jianjun Zang ◽  
Yunhe Cao

Abstract Background: Probiotics seem to be an alternative to antibiotics for improving animal's health and intestinal development. Saccharomyces boulardii ( S. boulardii ) is a well-known probiotic. However, only few studies have been performed examining the effects of S. boulardii on weaned piglets . Therefore, this study was conducted to investigate the effects of dietary S. boulardii mafic-1701 on growth performance, antioxidant parameters, inflammation and intestinal microbiota in weaned piglets, using aureomycin as positive control. One hundred and eight piglets were randomly divided into three dietary treatment groups: (1) basal diet (CON); (2) basal diet supplemented with 75 mg/kg aureomycin (ANT); (3) basal diet supplemented with 1 × 10 8 CFU/kg S. boulardii mafic-1701 (SB). Results: Compared to CON, the supplementation with S. boulardii mafic-1701 improved feed efficiency over the entire 28 days ( P < 0.01) and decreased the rate of diarrhea during the first week ( P < 0.05). Total superoxide dismutase concentration was markedly increased in piglets with S. boulardii mafic-1701 ( P < 0.01). Moreover, compared with CON, SB increased the concentration of interleukin-4 in ileum ( P < 0.05), while the level of pro-inflammatory cytokines interleukin-6 ( P < 0.01) and tumor necrosis factor ( P < 0.01) were decreased in jejunum. SB increased the abundance of Bacillus and Ruminococcaceae ( P < 0.05), whereas the population of Clostridiaceae were decreased ( P < 0.05). Furthermore, the analysis of microbiota metabolites showed that S. boulardii mafic-1701 administration increased the concentration of formate and isobutyrate in cecum to maintain a stable microbiota and gut health ( P < 0.05). Conclusion: This study indicated that S. boulardii mafic-1701 supplementation could improve growth performance, alleviate the severity of diarrhea in weaned piglets, which may be associated with S. boulardii mafic-1701 promoted antioxidant activity, anti-inflammatory responses and microbi al ecology of piglets.


Author(s):  
A. Biswas ◽  
R. Messam ◽  
M. Kumawat ◽  
M. Namit ◽  
A.B. Mandal ◽  
...  

A feeding trial was conducted to assess the effect of prebiotics on intestinal histo-morphometry and gut health status of broiler chickens. A total number of 240 day-old broiler chicks were randomly assigned to six dietary treatment groups having five replicates each with eight birds per replicate for six weeks. A basal corn-soybean diet was formulated that served as control (T1). The rest five test dietary treatments were prepared by adding antibiotic (bacitracin methylene di-salicylate-BMD at 0.002% of diet (T2), prebiotics i.e., mannan oligosaccharides (MOS) at 0.1% or 0.2% (T3 and T4) and fructo oligosaccharides (FOS) at 0.1% or 0.2% (T5, T6 respectively) with basal diet. Total anaerobes counts were significantly (P LESS THAN 0.05) reduced at 21 and 42 d at ileum in T2, T3 and T4 groups whereas, coliforms count were reduced (P LESS THAN 0.05) in T2 and T3 groups. However, lactic acid bacteria (LAB) count were significantly (P Less than 0.05) increased in T3 and T4 groups but lactobacillus count were increased (P less than 0.05) in only T3 group at 21 and 42 d in ileum. Villus height (VH) and width (VW) were significantly increased (P less than 0.05) at 21d and 42 d in T3 and T4 groups, whereas, at 21 d, crypt depth (CD) were increased (P less than 0.05) in T3 and T4 groups but at 42 d, CD were significantly improved in T3 and T4 or T5 and T6 groups. No significant (P>0.05) differences were observed in ratio between VH and CD at 21d or 42 d. Thus, it might he concluded that 0.1% or 0.2% MOS inclusion in basal diet improved intestinal histo-morphometry and gut health in broiler chickens and it is a better alternate feed additive to replacement of antibiotic growth promoter.


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 215-216
Author(s):  
Payton L Dahmer ◽  
Tamra Kott ◽  
Alison Crane ◽  
James Lattimer ◽  
Cassandra K Jones

Abstract Two experiments were conducted to evaluate corn dried distiller’s grains with solubles (DDGS) and corn gluten feed (CGF) as replacements for soybean meal (SBM) and determine the impact of an ionophore on Boer goat growth performance and carcass characteristics. In Exp. 1, 75 Boer-goat kids (initial BW 26.9 ± kg) were allotted to one of 5 dietary treatments: 1) Negative control (100% SBM, 0% DDGS and 0% CGF; 100SBM); 2) Positive control (100% DDGS, 0% CGF and 0% SBM; 100DDGS); 3) 66% DDGS, 33% CGF and 0% SBM (66DDGS/33CGF); 4) 66% CGF, 33% DDGS and 0% SBM (33DDGS/66 CGF); and 5) 100% CGF, 0% DDGS and 0% SBM (0DDGS/100CGF). Dietary treatment did not impact (P ≤ 0.21) growth. In Exp. 2, 72 Boer-goat kids (initial BW 21.7 ± kg) were allotted to one of 4 dietary treatments in a 2 X 2 factorial with 2 protein sources and 2 ionophore inclusions: 1) SBM/no ionophore (SBM-NI); Diet 2) SBM with ionophore (SBM-I); Diet 3) DDGS/no ionophore (DDGS-NI); and Diet 4) DDGS with ionophore (DDGS-I). On d 21, goats were split into 2 groups according to their protein source and fed an additional d 10 (Group 1: DDGS-I; Group 2: SBM-I). The 15 heaviest goats from each group were harvested and carcass data collected. The protein source X ionophore interaction was not significant (P ≥ 0.15) for any measured response criteria. Goats fed a diet with SBM had significantly increased (P = 0.04) ADG compared to goats fed DDGS. No evidence of difference was observed (P ³ 0.10) for ADFI or G:F. Dietary treatment did not impact (P &gt; 0.05) carcass characteristics. In summary, these data suggest that corn co-products can be economically included in Boer-goat diets, however their impact on growth performance is variable compared to that of soybean meal.


Author(s):  
Dianne Toe ◽  
Louise Paatsch ◽  
Amy Szarkowski

Deaf and hard-of-hearing (DHH) children who use spoken language face unique challenges when communicating with others who have typical hearing, particularly their peers. In such contexts, the social use of language has been recognized as an area of vulnerability among individuals in this population and has become a focus for research and intervention. The development of pragmatic skills intersects with many aspects of child development, including emotional intelligence and executive function, as well as social and emotional development. While all these areas are important, they are beyond the scope of this chapter, which highlights the impact of pragmatics on the specific area of cognition. Cognitive pragmatics is broadly defined as the study of the mental processes involved in the understanding of meaning in the context of a cooperative interaction. This chapter explores how DHH children and young people construe meaning in the context of conversations and expository interactions with their peers. The chapter aims to examine the role played by the cognitive processes of making inferences and comprehending implicature, within the overall display of pragmatic skills. Further, the authors use this lens in the analysis of interactions between DHH children and their peers in order to shed light on the development of pragmatic skills in children who are DHH.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anna Czech ◽  
Eugeniusz Ryszard Grela ◽  
Martyna Kiesz

AbstractThe aim of the study was to assess the effect of fermented dried soybean (FSBM) and/or fermented rapeseed meal (FRSM) in diets for weaned piglets on production results, nutrient digestibility, gastrointestinal tract histology, and the composition of the gut microbiota. Piglets in the control group received standard diets with soybean meal. Animals in all experimental groups received diets in which a portion of the soybean meal was replaced: in group FR—8% FRSM; in group FR/FS—6% FRSM and 2% FSBM; in group FS/FR—2% FRSM and 6% FSBM and in group FS—8% FSBM. The use of 8% FRSM or 6% FRSM and 2% FSBM in the piglet diets had a positive effect on average daily gains. Piglets from the FR and FR/FS groups had the highest feed conversion rate. Group FS/FR and FS piglets had significantly lower mortality and lower incidence of diarrhoea. Piglets fed a diet with the fermented components, in particular with 8% FRSM or 6% FRSM and 2% FSBM, exhibited a positive effect on the microbiological composition and histology of intestines, which resulted in improved nutrient digestibility coefficients (ATTD and AID).


Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 528
Author(s):  
Roswitha Siener ◽  
Norman Bitterlich ◽  
Hubert Birwé ◽  
Albrecht Hesse

Despite the importance of dietary management of cystinuria, data on the contribution of diet to urinary risk factors for cystine stone formation are limited. Studies on the physiological effects of diet on urinary cystine and cysteine excretion are lacking. Accordingly, 10 healthy men received three standardized diets for a period of five days each and collected daily 24 h urine. The Western-type diet (WD; 95 g/day protein) corresponded to usual dietary habits, whereas the mixed diet (MD; 65 g/day protein) and lacto-ovo-vegetarian diet (VD; 65 g/day protein) were calculated according to dietary reference intakes. With intake of the VD, urinary cystine and cysteine excretion decreased by 22 and 15%, respectively, compared to the WD, although the differences were not statistically significant. Urine pH was significantly highest on the VD. Regression analysis showed that urinary phosphate was significantly associated with cystine excretion, while urinary sulfate was a predictor of cysteine excretion. Neither urinary cystine nor cysteine excretion was affected by dietary sodium intake. A lacto-ovo-vegetarian diet is particularly suitable for the dietary treatment of cystinuria, since the additional alkali load may reduce the amount of required alkalizing agents.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 402
Author(s):  
Anne M.S. Huting ◽  
Anouschka Middelkoop ◽  
Xiaonan Guan ◽  
Francesc Molist

This is a comprehensive review on the use of nutritional strategies to shape the functioning of the gastro-intestinal tract in suckling and weaned piglets. The progressive development of a piglet’s gut and the associated microbiota and immune system offers a unique window of opportunity for supporting gut health through dietary modulation. This is particularly relevant for large litters, for which sow colostrum and milk are insufficient. The authors have therefore proposed the use of supplemental milk and creep feed with a dual purpose. In addition to providing nutrients to piglets, supplemental milk can also serve as a gut modulator in early life by incorporating functional ingredients with potential long-term benefits. To prepare piglets for weaning, it is important to stimulate the intake of solid feed before weaning, in addition to stimulating the number of piglets eating. The use of functional ingredients in creep feed and a transition diet around the time of weaning helps to habituate piglets to solid feed in general, while also preparing the gut for the digestion and fermentation of specific ingredients. In the first days after weaning (i.e., the acute phase), it is important to maintain high levels of feed intake and focus on nutritional strategies that support good gastric (barrier) function and that avoid overloading the impaired digestion and fermentation capacity of the piglets. In the subsequent maturation phase, the ratio of lysine to energy can be increased gradually in order to stimulate piglet growth. This is because the digestive and fermentation capacity of the piglets is more mature at this stage, thus allowing the inclusion of more fermentable fibres. Taken together, the nutritional strategies addressed in this review provide a structured approach to preparing piglets for success during weaning and the period that follows. The implementation of this approach and the insights to be developed through future research can help to achieve some of the most important goals in pig production: reducing piglet mortality, morbidity and antimicrobial use.


2020 ◽  
Vol 41 (S1) ◽  
pp. s258-s259
Author(s):  
James Harrigan ◽  
Ebbing Lautenbach ◽  
Emily Reesey ◽  
Magda Wernovsky ◽  
Pam Tolomeo ◽  
...  

Background: Clinically diagnosed ventilator-associated pneumonia (VAP) is common in the long-term acute-care hospital (LTACH) setting and may contribute to adverse ventilator-associated events (VAEs). Pseudomonas aeruginosa is a common causative organism of VAP. We evaluated the impact of respiratory P. aeruginosa colonization and bacterial community dominance, both diagnosed and undiagnosed, on subsequent P. aeruginosa VAP and VAE events during long-term acute care. Methods: We enrolled 83 patients on LTACH admission for ventilator weaning, performed longitudinal sampling of endotracheal aspirates followed by 16S rRNA gene sequencing (Illumina HiSeq), and bacterial community profiling (QIIME2). Statistical analysis was performed with R and Stan; mixed-effects models were fit to relate the abundance of respiratory Psa on admission to clinically diagnosed VAP and VAE events. Results: Of the 83 patients included, 12 were diagnosed with P. aeruginosa pneumonia during the 14 days prior to LTACH admission (known P. aeruginosa), and 22 additional patients received anti–P. aeruginosa antibiotics within 48 hours of admission (suspected P. aeruginosa); 49 patients had no known or suspected P. aeruginosa (unknown P. aeruginosa). Among the known P. aeruginosa group, all 12 patients had P. aeruginosa detectable by 16S sequencing, with elevated admission P. aeruginosa proportional abundance (median, 0.97; IQR, 0.33–1). Among the suspected P. aeruginosa group, all 22 patients had P. aeruginosa detectable by 16S sequencing, with a wide range of admission P. aeruginosa proportional abundance (median, 0.0088; IQR, 0.00012–0.31). Of the 49 patients in the unknown group, 47 also had detectable respiratory Psa, and many had high P. aeruginosa proportional abundance at admission (median, 0.014; IQR, 0.00025–0.52). Incident P. aeruginosa VAP was observed within 30 days in 4 of the known P. aeruginosa patients (33.3%), 5 of the suspected P. aeruginosa patients (22.7%), and 8 of the unknown P. aeruginosa patients (16.3%). VAE was observed within 30 days in 1 of the known P. aeruginosa patients (8.3%), 2 of the suspected P. aeruginosa patients (9.1%), and 1 of the unknown P. aeruginosa patients (2%). Admission P. aeruginosa abundance was positively associated with VAP and VAE risk in all groups, but the association only achieved statistical significance in the unknown group (type S error <0.002 for 30-day VAP and <0.011 for 30-day VAE). Conclusions: We identified a high prevalence of unrecognized respiratory P. aeruginosa colonization among patients admitted to LTACH for weaning from mechanical ventilation. The admission P. aeruginosa proportional abundance was strongly associated with increased risk of incident P. aeruginosa VAP among these patients.Funding: NoneDisclosures: None


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