scholarly journals Three Novel Bacteria Associated with Two Centric Diatom Species from the Mediterranean Sea, Thalassiosira rotula and Skeletonema marinoi

2021 ◽  
Vol 22 (24) ◽  
pp. 13199
Author(s):  
Federica Di Costanzo ◽  
Valeria Di Dato ◽  
Leonardo Joaquim van Zyl ◽  
Adele Cutignano ◽  
Francesco Esposito ◽  
...  
Keyword(s):  
Close Association ◽  
Antibiotic Resistance Genes ◽  
Gene Clusters ◽  
Biologically Active ◽  
Centric Diatom ◽  
Marine Food Web ◽  
Gulf Of Naples ◽  
The North ◽  
Successful Group ◽  
Skeletonema Marinoi

Diatoms are a successful group of microalgae at the base of the marine food web. For hundreds of millions of years, they have shared common habitats with bacteria, which favored the onset of interactions at different levels, potentially driving the synthesis of biologically active molecules. To unveil their presence, we sequenced the genomes of bacteria associated with the centric diatom Thalassiosira rotula from the Gulf of Naples. Annotation of the metagenome and its analysis allowed the reconstruction of three bacterial genomes that belong to currently undescribed species. Their investigation showed the existence of novel gene clusters coding for new polyketide molecules, antibiotics, antibiotic-resistance genes and an ectoine production pathway. Real-time PCR was used to investigate the association of these bacteria with three different diatom clones and revealed their preference for T. rotula FE80 and Skeletonema marinoi FE7, but not S. marinoi FE60 from the North Adriatic Sea. Additionally, we demonstrate that although all three bacteria could be detected in the culture supernatant (free-living), their number is up to 45 times higher in the cell associated fraction, suggesting a close association between these bacteria and their host. We demonstrate that axenic cultures of T. rotula are unable to grow in medium with low salinity (<28 ppt NaCl) whereas xenic cultures can tolerate up to 40 ppt NaCl with concomitant ectoine production, likely by the associated bacteria.

scholarly journals A captorhinid-dominated assemblage from the palaeoequatorial Permian of Menorca (Balearic Islands, western Mediterranean)

2021 ◽  
pp. 1-21
Author(s):  
Rafel MATAMALES-ANDREU ◽  
Francesc X. ROIG-MUNAR ◽  
Oriol OMS ◽  
Àngel GALOBART ◽  
Josep FORTUNY
Keyword(s):  
Western Mediterranean ◽  
Balearic Islands ◽  
Distribution Area ◽  
Lower Permian ◽  
Incertae Sedis ◽  
The North ◽  
Low Latitudes ◽  
High Fibre ◽  
Successful Group ◽  
Neighbouring Island

ABSTRACT Moradisaurine captorhinid eureptiles were a successful group of high-fibre herbivores that lived in the arid low latitudes of Pangaea during the Permian. Here we describe a palaeoassemblage from the Permian of Menorca (Balearic Islands, western Mediterranean), consisting of ichnites of small captorhinomorph eureptiles, probably moradisaurines (Hyloidichnus), and parareptiles (cf. Erpetopus), and bones of two different taxa of moradisaurines. The smallest of the two is not diagnostic beyond Moradisaurinae incertae sedis. The largest one, on the other hand, shows characters that are not present in any other known species of moradisaurine (densely ornamented maxillar teeth), and it is therefore described as Balearosaurus bombardensis gen. et sp. nov. Other remains found in the same outcrop are identified as cf. Balearosaurus bombardensis gen. et sp. nov., as they could also belong to the newly described taxon. This species is sister to the moradisaurine from the lower Permian of the neighbouring island of Mallorca, and is also closely related to the North American genus Rothianiscus. This makes it possible to suggest the hypothesis that the Variscan mountains, which separated North America from southern Europe during the Permian, were not a very important palaeobiogeographical barrier to the dispersion of moradisaurines. In fact, mapping all moradisaurine occurrences known so far, it is shown that their distribution area encompassed both sides of the Variscan mountains, essentially being restricted to the arid belt of palaeoequatorial Pangaea, where they probably outcompeted other herbivorous clades until they died out in the late Permian.

scholarly journals Evaluation of onion samples (Allium cepa L.) of various origins in the foothill zone of the North Caucasus and creation of source material fo

2021 ◽  
pp. 58-64
Author(s):  
I. S. Mastyaev ◽  
A. F. Agafonov ◽  
L. V. Krivenkov
Keyword(s):  
Climatic Conditions ◽  
Source Material ◽  
Biologically Active ◽  
F1 Hybrids ◽  
North Caucasus ◽  
F1 Hybrid ◽  
The North ◽  
Long Term Storage ◽  
Economically Valuable Traits ◽  
High Yields

Relevance. The success of breeding work is largely determined by the source material, the search and creation of which for target breeding begins with the collection and study of collectible samples for the alignment of morphological features; for the precocity and amity of maturation; for the keeping quality and transportability of bulbs; for the quality of vegetable products (high content of biologically active substances and antioxidants); for resistance to biotic and abiotic stressors of a specific growing zone.Materials and methods. On the basis of the North Caucasus branch FSBSI FSVC, 90 samples of spring onions from 26 countries of the world were evaluated in the number nursery for the period from 2017 to 2019, 71 of them were varieties and 19 were F1 hybrids. In a hybrid nursery, 25 hybrid combinations obtained from FSBSI FSVC (VNIISSOK) were evaluated. The laying and placement of experiments, agricultural techniques of cultivation, accounting and evaluation of economically valuable traits, biochemical analyses were carried out according to generally accepted methods. The standards are the new Primo and Ampex varieties of the selection of the FSBSI FSVC and the Italian F1 hybrid Ranko, which were placed every 10 collection samples.Results.According to the results of the assessment, among the studied F1 varieties and hybrids, sources of economically valuable traits such as yield (20-27 t/ha), precocity (80-90 days), preservation (more than 85%) when laid for long-term storage up to 7 months, purple, white and pink color of bulbs, as well as sources of long-shaped bulbs were identified. The greatest interest for breeding for yield is represented by 9 promising varieties and 9 F1 hybrids of onions of various origins, as well as 4 hybrid combinations that were distinguished by high yields, the yield of marketable products and the mass of marketable bulbs. These samples are recommended for use in the breeding process as sources for the creation of new varieties and hybrids with high yields for the agro-climatic conditions of the foothill zone of the North Caucasus.r breeding

scholarly journals Review on Bioactive and Antioxidant Potential of Coloured Fruits and Vegetables

2019 ◽  
Vol 9 (2) ◽  
pp. 433-441
Author(s):  
Sarvesh Patel ◽  
Manoj Kumar Chaubey ◽  
Ishwar Das ◽  
V. N. Pandey
Keyword(s):  
Fruits And Vegetables ◽  
Food Poisoning ◽  
Foot And Mouth Disease ◽  
Uttar Pradesh ◽  
Calorific Value ◽  
Biologically Active ◽  
Bioactive Substances ◽  
Nutritional Values ◽  
The North ◽  
North Eastern

The colour of fruits and vegetables represent a lot about their nutritional value. These nutritional values are due to presence of bioactive substances like vitamins, minerals, antioxidants and phytochemicals. Among natural dietary supplements, fruits and vegetables, in spite of low in calorific value, play very important role in human diet as a major source of biologically active compounds. Now a days, fruits and vegetables are gaining popularity and new ways of using as nutraceutical, antioxidants and medicines for treating diabetes, atherosclerosis, mastitis, cancer, Alzheimer’s disease, foot and mouth disease, gastric disorders, night blindness, skin allergies, hypersensitivity reaction, food poisoning, retention of placenta etc. The medicinal benefits are suitable for both the human as well as animals, being cost economic without side effect. The North Eastern Terai region of Uttar Pradesh harbors green lush vegetation having coloured fruits and vegetables. Till date 25 fruits and 21 vegetables plants are reported, which have enormous biological power and potential of nutraceutically active biomolecules. Therefore, the present study has undertaken to ascertain the possibilities of nutraceutical potential of coloured fruits and vegetables. Keywords; Nutraceuticals, Antioxidants, Phytochemicals, Bioactive molecule.

scholarly journals The resistome of common human pathogens

2017 ◽  
Author(s):  
Christian Munck ◽  
Mostafa M. Hashim Ellabaan ◽  
Michael Schantz Klausen ◽  
Morten O.A. Sommer
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Bacterial Pathogens ◽  
Antibiotic Resistance Genes ◽  
Gene Clusters ◽  
Species Level ◽  
Natural Environments ◽  
Human Pathogens ◽  
Bacterial Genomes ◽  
Risk Ranking

AbstractGenes capable of conferring resistance to clinically used antibiotics have been found in many different natural environments. However, a concise overview of the resistance genes found in common human bacterial pathogens is lacking, which complicates risk ranking of environmental reservoirs. Here, we present an analysis of potential antibiotic resistance genes in the 17 most common bacterial pathogens isolated from humans. We analyzed more than 20,000 bacterial genomes and defined a clinical resistome as the set of resistance genes found across these genomes. Using this database, we uncovered the co-occurrence frequencies of the resistance gene clusters within each species enabling identification of co-dissemination and co-selection patterns. The resistance genes identified in this study represent the subset of the environmental resistome that is clinically relevant and the dataset and approach provides a baseline for further investigations into the abundance of clinically relevant resistance genes across different environments. To facilitate an easy overview the data is presented at the species level at www.resistome.biosustain.dtu.dk.

scholarly journals BIOLOGICALLY ACTIVE PEPTIDES IN METABOLISM REGULATION. PEPTONS, PEPTIDES, AMINO ACIDS, FATTY ACIDS, LIPOPROTEINS, LIPIDS, AND THE EFFECT OF NUTRICEUTICALS

2019 ◽  
Vol 64 (1) ◽  
pp. 14-23 ◽  
Author(s):  
A. V. Aripovsky ◽  
V. N. Titov
Keyword(s):  
Homo Sapiens ◽  
Close Association ◽  
Biologically Active ◽  
Functional Difference ◽  
Small Molecular Weight ◽  
Active Peptides ◽  
Metabolism Regulation ◽  
General Pathology ◽  
Biologically Active Peptides

According to phylogenetic theory of general pathology, formation of multicellular organisms started when each cell (a unicellular organism) reached the first level of relative biological perfection. By that time the stimuli for perfection of the unicellular exhausted, and formation of the multicellular became a biological necessity. All cells, being associated, formed the second level of relative biological perfection within the principle of biological succession. The association included highly organized unicellular organisms with their specific autocrine biological functions and reactions. At the second level of relative biological perfection all humoral mediators in paracrine regulated cell communities (PC) and organs were predominantly hydrophilic and short living. They had a small molecular weight and were probably biologically active peptides (BAP). We believe that functional difference of PC and later of organs is based on differentiation of lysosomal function and production of various enzymes involved in proteolysis of dietary proteins. This allowed various PC and organs to form chemically and functionally different BAP pools from one protein upon proteolysis. Individual peptide pools in PC created the basis for morphologically and functionally different cells and organs. Cell that produces peptides can modify their concentration, chemical parameters and ratios by varying the selectivity of its proteases. In vivo regulation of metabolism by BAP has a common root in bacteria, plants and vertebrates, including Homo sapiens. The third level of relative biological perfection in the organism has formed in close association with cognitive biological function.

scholarly journals Secondary metabolites of a marine-derived Penicillium ochrochloron

2021 ◽  
Vol 13 (3) ◽  
pp. 11020
Author(s):  
Peter M. EZE ◽  
Ying GAO ◽  
Yang LIU ◽  
Lasse Van GEELEN ◽  
Chika P. EJIKEUGWU ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Biological Properties ◽  
Industrial Applications ◽  
Biologically Active ◽  
Fungal Isolation ◽  
The North ◽  
Wide Range ◽  
Extremophilic Fungi ◽  
Fungal Secondary Metabolites

Extremophilic fungi have received considerable attention recently as new promising sources of biologically active compounds with potential pharmaceutical applications. This study investigated the secondary metabolites of a marine-derived Penicillium ochrochloron isolated from underwater sea sand collected from the North Sea in St. Peter-Ording, Germany. Standard techniques were used for fungal isolation, taxonomic identification, fermentation, extraction, and isolation of fungal secondary metabolites. Chromatographic separation and spectroscopic analyses of the fungal secondary metabolites yielded eight compounds: talumarin A (1), aspergillumarin A (2), andrastin A (3), clavatol (4), 3-acetylphenol (5), methyl 2,5-dihydro-4-hydroxy-5-oxo-3-phenyl-2-furanpropanoate (6), emodin (7) and 2-chloroemodin (8). After co-cultivation with Bacillus subtilis, the fungus was induced to express (-)-striatisporolide A (9). Compound 1 was evaluated for antibacterial activity against Staphylococcus aureus, Acinetobacter baumannii, Mycobacterium smegmatis, and M. tuberculosis, as well as cytotoxicity against THP-1 cells. The compound, however, was not cytotoxic to THP-1 cells and had no antibacterial activity against the microorganisms tested. The compounds isolated from P. ochrochloron in this study are well-known compounds with a wide range of beneficial biological properties that can be explored for pharmaceutical, agricultural, or industrial applications. This study highlights the bioprospecting potential of marine fungi and confirms co-cultivation as a useful strategy for the discovery of new natural products.

scholarly journals Plant Species-Dependent Increased Abundance and Diversity of IncP-1 Plasmids in the Rhizosphere: New Insights Into Their Role and Ecology

2020 ◽  
Vol 11 ◽  
Author(s):  
Masaki Shintani ◽  
Eman Nour ◽  
Tarek Elsayed ◽  
Khald Blau ◽  
Inessa Wall ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Anthropogenic Pollution ◽  
Gene Clusters ◽  
Mobile Elements ◽  
Sequence Comparisons ◽  
Class 1 Integron ◽  
Class 1 ◽  
Abundance And Diversity

IncP-1 plasmids, first isolated from clinical specimens (R751, RP4), are recognized as important vectors spreading antibiotic resistance genes. The abundance of IncP-1 plasmids in the environment, previously reported, suggested a correlation with anthropogenic pollution. Unexpectedly, qPCR-based detection of IncP-1 plasmids revealed also an increased relative abundance of IncP-1 plasmids in total community DNA from the rhizosphere of lettuce and tomato plants grown in non-polluted soil along with plant age. Here we report the successful isolation of IncP-1 plasmids by exploiting their ability to mobilize plasmid pSM1890. IncP-1 plasmids were captured from the rhizosphere but not from bulk soil, and a high diversity was revealed by sequencing 14 different plasmids that were assigned to IncP-1β, δ, and ε subgroups. Although backbone genes were highly conserved and mobile elements or remnants as Tn501, IS1071, Tn402, or class 1 integron were carried by 13 of the sequenced IncP-1 plasmids, no antibiotic resistance genes were found. Instead, seven plasmids had a mer operon with Tn501-like transposon and five plasmids contained putative metabolic gene clusters linked to these mobile elements. In-depth sequence comparisons with previously known plasmids indicate that the IncP-1 plasmids captured from the rhizosphere are archetypes of those found in clinical isolates. Our findings that IncP-1 plasmids do not always carry accessory genes in unpolluted rhizospheres are important to understand the ecology and role of the IncP-1 plasmids in the natural environment.

THE DEVELOPMENT AND REGIONAL SIGNIFICANCE OF RIFT-RELATED DEPOSITIONAL SYSTEMS IN THE DAMPIER SUB-BASIN

1996 ◽  
Vol 36 (1) ◽  
pp. 369 ◽  
Author(s):  
L.R. Miller ◽  
S.A. Smith
Keyword(s):  
Time Scale ◽  
Close Association ◽  
Temporal Frequency ◽  
Absolute Error ◽  
Geological Time ◽  
Sediment Distribution ◽  
North West ◽  
The North ◽  
Depositional Systems ◽  
The Relationship

The influence of tectonic control is more apparent than eustatic control on the rift-related stratigraphy of the Dampier Sub-basin. The correlation of observed depositional events to causative processes and global events is problematic due to the use of alternative geological time scales, causing ambiguity and uncertainty. The Harland (1989) time scale with a revised palynological allocation, combined with genetic sequence stratigraphy, and Prosser's concept of the tectonic systems tract, has proved useful during evaluation of the stratigraphy of the Dampier Sub-basin.Palaeo-topography was a major factor in sediment distribution and facies architecture of rift-related strata in the Dampier Sub-basin. This must be considered when assessing the stratigraphic trapping potential for hydrocarbons. There is a close association between the styles of depositional systems observed in the Dampier Sub-basin and stage of rifting and basin development. Five tectonic systems tracts, each with unique depositional systems have been identified and described; pre-rift, rift initiation, rift climax, immediate post-rift and late post-rift tectonic systems tracts.The use of a single time scale has enhanced the relationship between tectonic systems tracts (super-cycles) and the timing of depositional events recorded during previous genetic stratigraphic studies in the North West Shelf. The tectonic nature of super-cycle scale events should be temporally and spatially assessed in detail before the effects of eustatic change are evaluated for rift-related successions of the North West Shelf. The problem can be further exacerbated when the absolute error of chronological dating exceed the temporal frequency of eustacy, causing tenuous correlations of depositional events to a global eustatic curve.

A Parapatric Boundary between Ranidella signifera and R. riparia (Anura: Leptodactylidae) in South Australia

1982 ◽  
Vol 30 (1) ◽  
pp. 49 ◽  
Author(s):  
FJ Odendaal ◽  
CM Bull
Keyword(s):  
Competitive Advantage ◽  
Close Association ◽  
South Australia ◽  
Wide Distribution ◽  
Habitat Types ◽  
South Eastern ◽  
The North ◽  
Flinders Ranges ◽  
Eastern Australia ◽  
Overlap Area

Ranidella signifera has a wide distribution in south-eastern Australia; R. riparia is endemic to the Flin- ders Ranges in South Australia. The ranges of the two species are largely allopatric, but they contact and overlap in a zone about 10 km wide, in the southern Flinders Ranges. The nature of the creeks changes across this zone. Immediately to the south and east, where only R. signifera is found, the creeks are slow-flowing and heavily vegetated, with mud or sand substrates. To the north and west the creeks are swift-flowing, and have rocky substrates and little vegetation; only R. riparia is found in these. In the sympatric overlap zone creeks are heterogeneous, with both habitat types represented. The close association between species and creek habitat is lost in populations not immediately adjacent to the overlap zone. This implies that each species can survive in both creek habitats but that R. riparia has a competitive advantage in swift, rocky creeks and R, signifera has an advantage in slow, vegetated creeks. This prevents either species from expanding its distribution beyond the narrow overlap area.

scholarly journals Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus

2020 ◽  
Vol 10 (9) ◽  
pp. 3417-3433
Author(s):  
Javier F Tabima ◽  
Ian A Trautman ◽  
Ying Chang ◽  
Yan Wang ◽  
Stephen Mondo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Secondary Metabolism ◽  
Gene Clusters ◽  
Biologically Active ◽  
Constitutive Gene Expression ◽  
Gut Symbionts ◽  
Phylogenomic Analyses ◽  
Siderophore Activity

Abstract Research into secondary metabolism (SM) production by fungi has resulted in the discovery of diverse, biologically active compounds with significant medicinal applications. The fungi rich in SM production are taxonomically concentrated in the subkingdom Dikarya, which comprises the phyla Ascomycota and Basidiomycota. Here, we explore the potential for SM production in Mucoromycota and Zoopagomycota, two phyla of nonflagellated fungi that are not members of Dikarya, by predicting and identifying core genes and gene clusters involved in SM. The majority of non-Dikarya have few genes and gene clusters involved in SM production except for the amphibian gut symbionts in the genus Basidiobolus. Basidiobolus genomes exhibit an enrichment of SM genes involved in siderophore, surfactin-like, and terpene cyclase production, all these with evidence of constitutive gene expression. Gene expression and chemical assays also confirm that Basidiobolus has significant siderophore activity. The expansion of SMs in Basidiobolus are partially due to horizontal gene transfer from bacteria, likely as a consequence of its ecology as an amphibian gut endosymbiont.

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