Optimizing the Extraction and Encapsulation of Mucilage from Brasenia Schreberi

The mucilage from Brasenia schreberi (BS) exhibits various biological activities, including antialgal, antibacterial, soluble-fiber properties, and excellent lubricating behavior. Thus, the extraction and wide use of mucilage in the food industry are crucial. In this study, the high-speed shear-assisted extraction of mucilage from BS was optimized by using response surface methodology (RSM). The optimal extraction conditions were as follows: Extraction temperature of 82 °C, extraction time of 113 min, liquid–solid ratio of 47 mL/g, and shear speed of 10,000 rpm. Under these conditions, the actual yield of BS mucilage was 71.67%, which highly matched the yield (73.44%) predicted by the regression model. Then, the BS mucilage extract was powdered to prepare the capsule, and the excipients of the capsule were screened using a single-factor test to improve the disintegration property and flowability. The final capsule formulation, which consisted of: 39% BS mucilage powder (60 meshes); 50% microcrystalline cellulose (60 meshes) as the filler; both 10% sodium starch glycolate and PVPP XL-10 (3:1, 60 meshes) as the disintegrant; both 1% colloidal silicon dioxide and sodium stearyl fumarate (1:1, 100 meshes) as the glidant by weight; were used for preparing the weights of a 320 mg/grain of capsule with 154.7 ± 0.95 mg/g polysaccharide content. Overall, the optimized extraction process had a high extraction rate for BS mucilage and the capsule formulation was designed reasonably.

Download Full-text

Optimization of Ultrasonic-Assisted Extraction of Polysaccharides from Ottelia acuminata (Gagnep.) Dandy Using RSM

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.396-398.292 ◽

2011 ◽

Vol 396-398 ◽

pp. 292-296

Author(s):

Ai Shi Zhu

Keyword(s):

Processing Parameters ◽

Extraction Time ◽

Extraction Temperature ◽

Independent Variables ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Technology ◽

Ultrasonic Assisted ◽

Experimental Yield ◽

Ultrasonic Assisted Extraction

Ultrasonic technology was applied to polysaccharides extraction from Ottelia acuminata (Gagnep.) Dandy and Response Surface Methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. Three independent variables such as liquid-solid ratio (ml/g, X1), extraction temperature (°C, X2) and extraction time (hour, X3) were investigated respectively. The statistical analysis indicated that the three variables and the quadratic of X1 and X3 had significant effects on the yields and followed by the significant interaction effects between the variables of X1 and X3, X2 and X3 (p<0.05). A mathematical model with high determination coefficient was gained. The optimal extraction conditions of polysaccharides were determined as follows: liquid-solid ratio 43 ml/g, extraction temperature 90 °C and extraction time 3.45 hours. Under these conditions, the experimental yield of polysaccharides was 107.44 mg/g, which was agreed closely with the predicted value 108.71 mg/g.

Download Full-text

Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

Applied Sciences ◽

10.3390/app10020560 ◽

2020 ◽

Vol 10 (2) ◽

pp. 560

Author(s):

Gregorio Iván Peredo Pozos ◽

Mario Alberto Ruiz-López ◽

Juan Francisco Zamora Nátera ◽

Carlos Álvarez Moya ◽

Lucia Barrientos Ramírez ◽

...

Keyword(s):

Antioxidant Activity ◽

Human Lymphocytes ◽

Dry Weight ◽

Extraction Process ◽

Hibiscus Sabdariffa ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

Download Full-text

Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization, HPLC Analysis, and Evaluation of Antioxidant Activity

Antioxidants ◽

10.3390/antiox8100425 ◽

2019 ◽

Vol 8 (10) ◽

pp. 425 ◽

Cited By ~ 4

Author(s):

Mengyang Hou ◽

Wenzhong Hu ◽

Aosheng Wang ◽

Zhilong Xiu ◽

Yusheng Shi ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Pteris Cretica ◽

Nitric Oxide Radical ◽

Ultrasonic Assisted ◽

Ic50 Values

In the present work, the ultrasonic-assisted extraction (UAE) of total flavonoids (TF) from Pteris cretica L. was optimized by response surface methodology (RSM) on the basis of a single-factor experiment. The optimized UAE parameters were as follows: Ethanol concentration 56.74%, extraction time 45.94 min, extraction temperature 74.27 °C, and liquid/solid ratio 33.69 mL/g. Under the optimized conditions, the total flavonoids yield (TFY) was 4.71 ± 0.04%, which was higher than that obtained by heat reflux extraction (HRE). The extracts were further analyzed by HPLC, and five major flavonoids, including rutin, quercitrin, luteolin, apigenin, and luteolin-7-O-glucoside, were identified and quantified. Furthermore, the results of the antioxidant test showed that the TF extract obtained under optimized UAE conditions exhibited good 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS+•), nitric oxide radical (NO•) scavenging activities, and ferrous ion (Fe2+) chelating capacity, with IC50 values of 74.49, 82.92, 89.12, and 713.41 µg/mL, respectively. Results indicated that the UAE technique developed in this work was an efficient, rapid, and simple approach for the extraction of flavonoids with antioxidant activity from P. cretica.

Download Full-text

Optimization of Ultrasound-Assisted Extraction of Antioxidant Polyphenols from the Seed Coats of Red Sword Bean (Canavalia gladiate (Jacq.) DC.)

Antioxidants ◽

10.3390/antiox8070200 ◽

2019 ◽

Vol 8 (7) ◽

pp. 200 ◽

Cited By ~ 5

Author(s):

Yue Zhou ◽

Xiao-Yu Xu ◽

Ren-You Gan ◽

Jie Zheng ◽

Ya Li ◽

...

Keyword(s):

Extraction Method ◽

Natural Antioxidants ◽

Extraction Time ◽

Antioxidant Compounds ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Seed Coats

The seed coat of red sword bean (Canavalia gladiata (Jacq.) DC.) is rich in antioxidant polyphenols. It is often discarded as a byproduct with the consumption of red sword bean, since it is very thick and not consumed by people. The aim of this study was to develop an ultrasound-assisted extraction method to extract natural antioxidants from the seed coats. The extraction process was optimized by using response surface methodology. After the single-factor experiments, three key factors, including ethanol concentration, liquid/solid ratio, and extraction time, were selected and their interactions were studied using a central composite design. The optimal extraction condition was 60.2% hydroethanol, a liquid/solid ratio of 29.3 mL/g, an extraction time of 18.4 min, an extraction temperature of 50 °C, and ultrasound power of 400 W. Under the optimal conditions, antioxidant activity of the extract was 755.98 ± 10.23 μmol Trolox/g dry weight (DW), much higher than that from maceration (558.77 ± 14.42 μmol Trolox/g DW) or Soxhlet extraction (479.81 ± 12.75 μmol Trolox/g DW). In addition, the main antioxidant compounds in the extract were identified and quantified by high-performance liquid chromatography–diode array detection–tandem mass spectrometry (HPLC–DAD–MS/MS). The concentrations of digalloyl hexoside, methyl gallate, gallic acid, trigalloyl hexoside, and digallic acid were 15.30 ± 0.98, 8.85 ± 0.51, 8.76 ± 0.36, 4.27 ± 0.21, and 2.89 ± 0.13 mg/g DW. This study provides an efficient and green extraction method for the extraction of natural antioxidants from the bean coat of red sword bean. The extract of antioxidants might be added into functional foods or nutraceuticals with potential beneficial functions.

Download Full-text

Optimization, Modeling and Antibacterial Activity of Extraction from Fructus Alpinia Oxyphylla

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.827 ◽

2013 ◽

Vol 781-784 ◽

pp. 827-831

Author(s):

Dan Wang ◽

Wen Xue Chen ◽

Yue Ying Hu ◽

Mei Fang Peng

Keyword(s):

Model Simulation ◽

Effective Diffusion ◽

Antibacterial Activities ◽

Extraction Process ◽

Actual Process ◽

Extraction Temperature ◽

Solid Ratio ◽

Alpinia Oxyphylla ◽

Solvent Density ◽

Good Agreement

Substances from Fructus Alpinia Oxyphylla (AOF) were extracted by using solvent extraction. In accordance with central composited design of response surface methodology system, the extracts were analyzed for the index which is the criteria formula of multiplying OD value by the yield. The optimum conditions obtained were extraction temperature of 60°C, liquid-solid ratio of 9mL/g, and solvent density of 80%. According to Ficks second law of diffusion, experimental parameters of K (rate constant), Ea (activation energy), Gs (effective diffusion coefficient) were gained, which represents a good agreement between the model simulation and the results of the actual process. The results can provide the valuable theory basis for the technical design and further research of extraction process. Antibacterial activities of the extraction were shown through MIC of which represents a significant effect.

Download Full-text

Optimization of Ultrasound-Assisted Extraction on Antioxidative Activity of Malus toringoides Using Response Surface Methodology

Processes ◽

10.3390/pr7050270 ◽

2019 ◽

Vol 7 (5) ◽

pp. 270 ◽

Cited By ~ 1

Author(s):

Qiang Ye ◽

Li Guo ◽

Hongmei Liu ◽

Yushi Liu ◽

Cunyan Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Response Surface Methodology ◽

Response Surface ◽

Umbilical Vein ◽

Extraction Temperature ◽

Least Squares Regression ◽

Protective Activity ◽

Ultrasound Assisted Extraction ◽

Solid Ratio ◽

Assisted Extraction

Ultrasonic-assisted extraction (UAE) was optimized using response surface methodology (RSM) to maintain the cyto-protective activity of M.toringoides against oxidative stress. The optimal conditions for UAE were a 58 mL/g liquid-solid ratio, a 38 °C extraction temperature, an 85% solvent concentration, and a 19-min extraction time, which resulted in a protection rate of 54.57% against hydrogen peroxide-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). These results were comparable to the predicted value of 53.75%. The extracts showed excellent antioxidant activity, and phlorizin was detected in the dried leaves of Malus.toringoides. The highest yield of phlorizin (101.239 mg/g) was also obtained using these conditions. Taken together, these results showed that the method successfully integrated RSM and partial least squares regression methods to optimize M.toringoides extraction to yield the highest cyto-protective activity and effectively increase the yield of phlorizin from M.toringoides.

Download Full-text

Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Moringa oleifera Leaves Using Response Surface Methodology

Asian Journal of Research in Chemistry ◽

10.52711/0974-4150.2021.00062 ◽

2021 ◽

pp. 363-367

Author(s):

Abdelhakim Benarima ◽

Mohamed Nasreddine Raache ◽

Moulay Rachid Kouadri ◽

Yassine Belaiche ◽

Salah Eddine Laouini

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Moringa Oleifera ◽

Extraction Time ◽

Extraction Temperature ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Moringa Oleifera Leaves ◽

Ultrasonic Assisted Extraction

Moringa oleifera is considered one of the most useful plants in the world because it's rich in bioactive substances, which employing on modern medical treatment, also can be used for many fields: pharmaceutical, food and cosmetics purposes. In this study, the response surface methodology (RSM) based on a Box–Behnken design (BBD) was employed to optimize the extraction time (X1: 20–60 min), extraction temperature (X2: 15–45 °C) and solvent-solid ratio (X3: 5–7 ml/g), to obtain a high crude of flavonoids yield from Moringa oleifera Leaves by ultrasonic-assisted extraction technique (UAE). The optimum conditions were an extraction time 23 (min), extraction temperature 44 (°C) and solvent-solid ratio 5.05 (ml/g). Under these conditions, the experimental yield was 72.65 (mg ER/g), well matched with the predicted yield 74.34 (mg ER/g) with the coefficients of determination (R2= 0.9861), thus indicating the suitability of response surface methodology in optimizing the ultrasound-assisted extraction of flavonoids from Moringa oleifera Leaves.

Download Full-text

Study on Microwave-Assisted Extraction of Polysaccharides from Radix astragali

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.675-677.1634 ◽

2014 ◽

Vol 675-677 ◽

pp. 1634-1637 ◽

Cited By ~ 1

Author(s):

Cong Liang Huang

Keyword(s):

Irradiation Time ◽

Good Alternative ◽

Extraction Yield ◽

Extraction Temperature ◽

Microwave Assisted Extraction ◽

Radix Astragali ◽

Solid Ratio ◽

Microwave Assisted ◽

Assisted Extraction ◽

Orthogonal Test Design

In this study, microwave-assisted extraction (MAE) was employed for the extraction of polysaccharides from Radix Astragali (PRA). The extraction parameters that influenced the extraction yield of PRA were optimized by orthogonal test design. The results showed that the optimal MAE conditions were as followings: liquid-solid ratio of 18 ml/g, irradiation time of 8 min, irradiation power of 300 W and extraction temperature of 65°C, while extraction yield of PRA was 8.39 %. The developed MAE method provided a good alternative for the extraction of PRA.

Download Full-text

Identification and Antioxidant Activity of Flavonoids Extracted from Xinjiang Jujube (Ziziphus jujube Mill.) Leaves with Ultra-High Pressure Extraction Technology

Molecules ◽

10.3390/molecules24010122 ◽

2018 ◽

Vol 24 (1) ◽

pp. 122 ◽

Cited By ~ 13

Author(s):

Li Zhang ◽

Pengzhan Liu ◽

Linlin Li ◽

Ying Huang ◽

Yunfeng Pu ◽

...

Keyword(s):

High Pressure ◽

Large Scale ◽

Radical Scavenging ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Extraction Technology ◽

Solid Ratio ◽

Ultra High Pressure ◽

Assisted Extraction ◽

Pressure Extraction

In this study, the ultra-high pressure extraction (UHPE) conditions for obtaining the maximum flavonoid yield from Xinjiang jujube (Ziziphus jujuba Mill.) leaves (XJL) were optimized by response surface methodology (RSM). Box–Behnken design (BBD) was applied to evaluate the effects of four variables (extraction temperature, pressure, time and liquid-to-solid ratio) on flavonoid yield. The results showed that the optimal flavonoid yield (25.45 ± 0.21 mg/g) was derived at 50.0 °C, 342.39 MPa, 11.56 min, and a liquid-to-solid ratio of 43.95 mL/g. Eight compounds were tentatively identified and quantified as kaempferol and quercetin glycosides with UPLC-ESI-MS. Compared to ultrasound-assisted extraction (UAE), UHPE can obtain higher concentrations of total flavonoids and stronger DPPH and ABTS radical-scavenging activities in a much shorter time. Therefore, UHPE is an alternative to UAE for obtaining flavonoids from XJL, which may be an optional method for large-scale industrial flavonoid extraction from XJL.

Download Full-text

Concurrent Extraction and Purification of Gentiopicroside from Gentiana scabra Bunge Using Microwave-Assisted Ethanol-Salt Aqueous Two-Phase Systems

Journal of Chromatographic Science ◽

10.1093/chromsci/bmz101 ◽

2019 ◽

Vol 58 (1) ◽

pp. 60-74

Author(s):

Zhenyu Cheng ◽

Haiyan Song ◽

Yuewei Zhang ◽

Dandan Han ◽

Xue Yu ◽

...

Keyword(s):

Potential Method ◽

Extraction Yield ◽

Phase System ◽

Extraction Temperature ◽

Two Phase ◽

Gentiana Scabra ◽

Solid Ratio ◽

Microwave Assisted ◽

Extraction And Purification ◽

Assisted Extraction

Abstract A potential method called microwave-assisted aqueous two-phase extraction (MA-ATPE) was developed for concurrent extraction and purification of gentiopicroside from Gentiana scabra Bunge. Formation characteristics of aqueous two-phase system (ATPS) composed of ethanol and 25 kinds of salts were investigated; K2HPO4 (w/w, 21.71%) and ethanol (w/w, 40.72%) were determined to be the optimal compositions of ATPS. Response surface methodology based on Box–Behnken design was used to investigate the extraction conditions, the optimal parameters were summarized as follows: 80°C of extraction temperature, 31 s of extraction time, 11:1 (mL/g) of liquid-to-solid ratio, 100 meshes of particle size and 806 W of microwave power. Under these conditions, the extraction yield of gentiopicroside was 65.32 ± 0.24 mg/g with a recovery of 96.51%. Compared with other four methods, the purity of gentiopicroside in the crude extracts reached 17.16 ± 0.25%, which was significantly higher than that of smashing tissue extraction, microwave assisted-extraction, ultrasonic-assisted extraction and heat reflux extraction, respectively. In addition, the phase-forming salt can be recyclable. Therefore, MA-ATPE was an excellent and alternative technique to the conventional extraction approaches of gentiopicroside.

Download Full-text