Phytochemical Constituents Identified from the Aerial Parts of Lespedeza cuneata and Their Effects on Lipid Metabolism during Adipocyte Maturation

Lespedeza cuneata, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural compounds with biological activities from Korean medicinal plants, the phytochemical investigation of L. cuneata resulted in the isolation of five chemical constituents: α-tocopherol (1), 7a-methoxy-α-tocopherol (2), 13(R)-hydroxy-octadeca-(9Z,11E,15Z)-trien-oic acid (3), α-dimorphecolic acid (4), and lupeol (5). The structural determination of the isolated compounds was elucidated from data gathered through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography–mass spectrometry (LC/MS). Until now, this study is the first to report these five compounds from the plant L. cuneata. Moreover, these isolated compounds (1–5) were evaluated for their anti-adipogenesis effects and their role in lipid metabolism during adipocyte maturation. As a result, the upregulation of mRNA expression levels of Fabp4 from 3T3-L1 pre-adipocytes treated with compounds 3 and 4 demonstrated that these compounds efficiently induced adipocyte differentiation. Furthermore, compounds 3 and 4 were found to regulate lipid metabolism by the induction of lipolytic and of lipogenic gene expressions. Therefore, experimental data from these findings supported that the compounds 3 and 4 induce the adipogenesis of 3T3-L1 pre-adipocytes and regulate lipid metabolism.

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ISOLATION AND IDENTIFICATION OF NON-POLAR CHEMICAL ENTITY FROM LEPTADENIA RETICULATA AERIAL PARTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i2.28683 ◽

2019 ◽

pp. 226-229

Author(s):

JAYESH DHALANI ◽

PANKAJ NARIYA

Keyword(s):

Biological Activities ◽

Petroleum Ether Extract ◽

Stem Bark ◽

Chemical Entity ◽

Resonance Spectroscopy ◽

Chromatographic Technique ◽

Isolation And Identification ◽

Aerial Parts ◽

Phytochemical Constituents

Objective: Leptadenia reticulata is the medicinal plant having many biological activities. It is necessary to find out which types of phytochemical constituents are present in the plant. The objective of this investigation was to isolate and identify the non-polar chemical entity of the areal parts of L. reticulata a plant used for medicinal purpose in folklore. Methods: Petroleum ether extract of the stem bark was used for this study. Non-polar solvent was used to extract non-polar chemical entity from areal parts of leptadeniareticulata. Through the saponification process, saponifiable and unsaponifiable matter was separated. Phytochemical constituents were separated using column chromatography. Separated fractions were analyzed on gas chromatography and mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Results: Hentriacontane compound was isolated and confirmed from GC-MS and NMR whereas phytol, Lupeol, β-amyrin, Campesterol, Stigmasterol, gamma-sitosterol identified through GC and mass spectroscopy. Conclusion: The present study showed that L. reticulata areal parts three sterols chemical entity Campesterol, Stigmasterol, gamma-sitosterol and phytol (diterpene alcohol), lupeol (triterpenoid), beta-amyrin (triterpene), and hentriacontane (alkane hydrocarbon). Core determination of the experiment was the development efficient method to isolate or identify the non-polar chemical entity through chromatographic technique.

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Chemical Constituents and Biological Activities of Galinsoga parvifl ora Cav. (Asteraceae) from Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2013-7-805 ◽

2013 ◽

Vol 68 (7-8) ◽

pp. 285-292 ◽

Cited By ~ 1

Author(s):

Islam Mostafa ◽

Ehsan Abd El-Aziz ◽

Samia Hafez ◽

Assem El-Shazly

Keyword(s):

Ethyl Acetate ◽

Biological Activities ◽

Chemical Constituents ◽

Enzyme Level ◽

Ethanolic Extract ◽

Ethyl Acetate Fraction ◽

Diabetic Rats ◽

Isolation And Identification ◽

Aerial Parts ◽

Phytochemical Investigation

The phytochemical investigation of an aqueous ethanolic extract of Galinsoga parviflora Cav. (Asteraceae) resulted in the isolation and identification of eleven compounds namely: triacontanol, phytol, β-sitosterol, stigmasterol, 7-hydroxy-β-sitosterol, 7-hydroxystigmasterol, β-sitosterol-3-O-β-D-glucoside, 3,4-dimethoxycinnamic acid, protocatechuic acid, fumaric acid, and uracil. Furthermore, 48 volatile constituents were identified in the hydrodistilled oil of the aerial parts. The ethanolic extract at a content of 400 mg/kg body weight (BW) exerted 87% reduction in the alanine aminotransferase enzyme level in cirrhotic rats compared with the standard silymarin (150 mg/kg BW) and also exerted a reduction in the blood glucose level equivalent to that of glibenclamide (5 mg/kg BW) in diabetic rats. The ethanolic extract, light petroleum and ethyl acetate fractions exhibited substantial antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Aspergillus niger, and Candida albicans. The ethyl acetate fraction showed strong antioxidant activity at a concentration of 150 mg/mL as compared with 0.1 M ascorbic acid. The cytotoxic effect against the MCF-7 cell line was found to be weak

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Phytochemical Constituents of Annona reticulata and their Cytotoxic Activity

Letters in Organic Chemistry ◽

10.2174/1570178616666190724123004 ◽

2020 ◽

Vol 17 (3) ◽

pp. 206-210

Author(s):

Ty Viet Pham ◽

Thang Quoc Le ◽

Anh Tuan Le ◽

Hung Quoc Vo ◽

Duc Viet Ho

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Structural Determination ◽

Phytochemical Investigation ◽

Ic50 Values ◽

Phytochemical Constituents ◽

First Time ◽

Annona Reticulata

A phytochemical investigation of the leaves of Annona reticulata led to the isolation and structural determination of β-sitosterol (1), ent-pimara-8(14),15-dien-19-oic acid (2), ent-pimara- 8(14),15-dien-19-ol (3), quercetin (4), quercetin 3-O-α-L-arabinopyranoside (5), and a mixture of quercetin 3-O-β-D-galactopyranoside (6a) and quercetin 3-O-β-D-glucopyranoside (6b). Of these, compounds 2 and 3 were isolated from the genus Annona for the first time. Compound 3 showed strong cytotoxicity against SK-LU-1 and SW626 cell lines with IC50 values of 17.64 ± 1.07 and 19.79 ± 1.41 μg mL-1, respectively.

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Update on Phytochemical and Biological Studies on Rocaglate Derivatives from Aglaia Species

Planta Medica ◽

10.1055/a-1401-9562 ◽

2021 ◽

Author(s):

Garima Agarwal ◽

Long-Sheng Chang ◽

Djaja Doel Soejarto ◽

A. Douglas Kinghorn

Keyword(s):

Biological Activities ◽

Chemical Constituents ◽

Protein Translation ◽

Biological Properties ◽

Translation Initiation Factor ◽

Initiation Factor ◽

Eukaryotic Translation ◽

Ability To Act ◽

Biological Studies ◽

Phytochemical Constituents

AbstractWith about 120 species, Aglaia is one of the largest genera of the plant family Meliaceae (the mahogany plants). It is native to the tropical rainforests of the Indo-Australian region, ranging from India and Sri Lanka eastward to Polynesia and Micronesia. Various Aglaia species have been investigated since the 1960s for their phytochemical constituents and biological properties, with the cyclopenta[b]benzofurans (rocaglates or flavaglines) being of particular interest. Phytochemists, medicinal chemists, and biologists have conducted extensive research in establishing these secondary metabolites as potential lead compounds with antineoplastic and antiviral effects, among others. The varied biological properties of rocaglates can be attributed to their unusual structures and their ability to act as inhibitors of the eukaryotic translation initiation factor 4A (eIF4A), affecting protein translation. The present review provides an update on the recently reported phytochemical constituents of Aglaia species, focusing on rocaglate derivatives. Furthermore, laboratory work performed on investigating the biological activities of these chemical constituents is also covered.

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The Phytochemistry of Cherokee Aromatic Medicinal Plants

Medicines ◽

10.3390/medicines5040121 ◽

2018 ◽

Vol 5 (4) ◽

pp. 121 ◽

Cited By ~ 1

Author(s):

William Setzer

Keyword(s):

Native Americans ◽

Medicinal Plants ◽

Biological Activities ◽

Herbal Medicines ◽

Black Cohosh ◽

Cimicifuga Racemosa ◽

Traditional Uses ◽

Aromatic And Medicinal Plants ◽

Phytochemical Investigation ◽

Phytochemical Constituents

Background: Native Americans have had a rich ethnobotanical heritage for treating diseases, ailments, and injuries. Cherokee traditional medicine has provided numerous aromatic and medicinal plants that not only were used by the Cherokee people, but were also adopted for use by European settlers in North America. Methods: The aim of this review was to examine the Cherokee ethnobotanical literature and the published phytochemical investigations on Cherokee medicinal plants and to correlate phytochemical constituents with traditional uses and biological activities. Results: Several Cherokee medicinal plants are still in use today as herbal medicines, including, for example, yarrow (Achillea millefolium), black cohosh (Cimicifuga racemosa), American ginseng (Panax quinquefolius), and blue skullcap (Scutellaria lateriflora). This review presents a summary of the traditional uses, phytochemical constituents, and biological activities of Cherokee aromatic and medicinal plants. Conclusions: The list is not complete, however, as there is still much work needed in phytochemical investigation and pharmacological evaluation of many traditional herbal medicines.

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PHYTOCHEMICAL CONSTITUENTS AND PHARMACOLOGICAL ACTIVITIES OF Kigelia africana AND Mansoa alliacea - A COMPREHENSIVE REVIEW

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s4.31703 ◽

2018 ◽

Vol 11 ◽

Author(s):

Sowmyalakshmi Venkataraman ◽

Gunda Srilakshmi

Keyword(s):

Rural Areas ◽

Biological Activities ◽

Chemical Constituents ◽

Reproductive Organ ◽

Human Beings ◽

Pharmacological Activities ◽

Phytochemical Constituents ◽

Athlete’S Foot ◽

Collective Information

Context: The focus of the present review is to compile the different phytochemical constituents and pharmacological activities of bignoniaceous plants, namely Kigelia africana and Mansoa alliacea. Objective: Medicinal plants are routinely used in clinical practice for the treatment of various diseases of human beings over thousands of years across the world. In rural areas of the developing countries, they are still used in practice due to the easy availability of the medication. The main objective of this work is to explore the phytochemical studies and biological activities of plants belonging to Bignoniaceae family. Methods: The present study also discusses about the different phytochemical constituents of K. africana and M. alliacea that were isolated and characterized using various analytical methods. Different extracts of these plants were subjected to different in vitro anticancer, analgesic, antimicrobial, and antimalarial activities using earlier reported methods were also discussed. Results: K. africana is commonly referred to as sausage or cucumber tree due to its huge sausage or cucumber-like fruit. These plants find application as traditional medicine for treating several ailments such as malignant tumor, ulcer, aging, and malaria among others. It is conjointly used for the treatment of reproductive organ infections, renal ailments, dizziness, epilepsy, sickle cell disease, depression, metabolism disorders, skin grievance, leprosy, impetigo, helminthic infections, athlete’s foot, tumors, etc. These plant extracts have also been used as a cosmetic by enhancing the collagen action and thus exhibit antiaging properties. Conclusion: The present study attempts to provide collective information on various phytochemical constituents and pharmacological actions of K. africana and M. alliacea belonging to Bignoniaceae. This review in its present form is believed to help the researchers to provide adequate information about the chemical constituents and biological uses of these plants.

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Phytochemical Investigation of Aerial Parts of Iraqi Cardaria draba

Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) ◽

10.31351/vol29iss2pp27-36 ◽

2020 ◽

Vol 29 (2) ◽

pp. 27-36

Author(s):

Alaa M. Abd ◽

Enas J. Kadhim

Keyword(s):

High Performance Liquid Chromatography ◽

Phenolic Acid ◽

High Performance ◽

Chemical Constituents ◽

Aqueous Methanol ◽

Plant Materials ◽

Aerial Parts ◽

Phytochemical Investigation ◽

Soxhlet Apparatus ◽

Layer Chromatography

The aim of this study was to study chemical constituents of aerial parts of Cardaria draba since no phytochemical investigation had been studied before in Iraq. Aerial parts of Cardaria draba were defatted by maceration in hexane for 72 h. The defatted plant materials were extracted using Soxhlet apparatus, the aqueous Methanol 90% as a solvent extraction for 18 h, and fractionated with petroleum ether- chloroform (CHCl3)- ethylacetate- and n-butanol respectivly. The ethyl acetate, n-butanol, and n-butanol after hydrolysis fractions were investigated by high performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) for its phenolic acid and flavonoid contents. Flavonoids and phenolic acid derivative were isolated from the ethylacetate of leaf fraction and n-butanol after hydrolysis fraction of the aerial parts and identified by TLC, FTIR and HPLC. A various chromatographic and spectroscopic results shown the presence of luteolin, chlorogenic acid, caffeic acid, and resorcinol in aerial parts of C. draba.

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STUDY ON CHEMICAL CONSTITUENTS AND CYTOTOXICITY OF ASPIDISTRA LETREAE

HUE UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCE ◽

10.26459/hueuni-jns.v129i2a.5656 ◽

2020 ◽

Vol 129 (1B) ◽

Author(s):

Duc Viet Ho

Keyword(s):

Methanol Extract ◽

Human Cancer ◽

Chemical Constituents ◽

Hep G2 ◽

Pure Compound ◽

Human Cancer Cell Lines ◽

Phytochemical Investigation ◽

Reported Data ◽

First Time

A phytochemical investigation of whole Aspidistra letreae plants led to the isolation of 2H-chromen-2-one (1), α-tocopherol (2), (E)-phytol (3), asparenydiol (4) and (25S)-spirost-1β,3α,5β-triol (5). Their structures were determined on the basis of NMR spectral evidences and in comparison with the reported data. Of these, asparenydiol (4) was isolated from the genus Aspidistra for the first time. This is also the first report on the separation and structural determination of (25S)-spirost-1β,3α,5β-triol (5) as a pure compound. The methanol extract exhibited a moderate cytotoxicity against the LU-1, HeLa, MDA-MB-231, Hep-G2, and MKN-7 human cancer cell lines with IC50 values ranging from 52.58 ± 3.65 to 64.78 ± 4.89 μg/mL.

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COMPONENT COMPOSITION AND BIOLOGICAL ACTIVITY OF GENUS VIOLA (VIOLACEAE) OF RUSSIAN FLO-RA

chemistry of plant raw material ◽

10.14258/jcprm.2020026305 ◽

2020 ◽

pp. 19-45

Author(s):

Natal'ya Valeriyevna Petrova ◽

Nina Anatol'yevna Medvedeva

Keyword(s):

Biological Activity ◽

Biological Activities ◽

Chemical Constituents ◽

Component Composition ◽

The Past ◽

Aerial Parts ◽

Different Types ◽

The Subject ◽

The Russian Federation ◽

Chemical Formulas

The subject of the review is the component composition and biological activity of species of genus Viola L. (Violaceae) from the flora of the Russian Federation according publications appeared over the past few decades. Chemical constituents and biological activities have been reported for 24 species of the 100 Viola species occurring in Russian. Components of various structures: flavonoids, cyclotides, anthocyanidins, phenylpropanoic acids, coumarins, alkaloids, fatty acids and its derivatives and ect. were found in the roots, aerial parts, flowers and leaves. Composition of 137 flavonoids, alkaloids, coumarins etc. was expanded with references to their chemical formulas and literature sources. Data of the essential oils isolated from the leaves of V. tricolor L., V. arvensis Murray, V. yedoensis Makino и V. odorata L. is systematized. It was demonstrated that extracts, their fractions, and some components showed different types of biological activity, including anti-inflammatory, cytotoxic and antiviral ones. The revealed range of biological activity partially confirmed expediency of using species of genus Viola in folk and standard practice medicine.

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Chemical constituents and cytotoxicity of Aspidistra letreae

HUE UNIVERSITY JOURNAL OF SCIENCE NATURAL SCIENCE ◽

10.26459/hueuni-jns.v129i1b.5656 ◽

2020 ◽

Vol 129 (1B) ◽

pp. 31-39

Author(s):

Duc Viet Ho ◽

Hanh Nhu Thi Hoang ◽

Khue Minh Vo ◽

Anh Tuan Le ◽

Hoai Thi Nguyen

Keyword(s):

Human Cancer ◽

Chemical Constituents ◽

Hep G2 ◽

Human Cancer Cell Lines ◽

Phytochemical Investigation ◽

Ic50 Values ◽

Reported Data ◽

Whole Plants ◽

First Time

A phytochemical investigation of whole Aspidistra letreae plants led to the isolation of 2H-chromen-2-one (1), α-tocopherol (2), (E)-phytol (3), asparenydiol (4) and (25S)-spirost-1β,3α,5β-triol (5). Their structures were determined on the basis of NMR spectral evidences and in comparison with the reported data. Of these, asparenydiol (4) was isolated from the genus Aspidistra for the first time. This is also the first report on the separation and structural determination of (25S)-spirost-1β,3α,5β-triol (5) as a pure compound. The methanol extract from the whole plants of Aspidistra letreae exhibits moderate cytotoxicity against the LU-1, HeLa, MDA-MB-231, Hep-G2, and MKN-7 human cancer cell lines with IC50 values ranging from 52.58 ± 3.65 to 64.78 ± 4.89 μg/mL.

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