scholarly journals The Effect of Eight Weeks of Testosterone Enanthate Consumption on Oxidative Indicators of Kidney Tissue in Resistance Trained Rats

2020 ◽  
Vol 5 (4) ◽  
pp. 155-159
Author(s):  
Masoumeh Mehrabi ◽  
Yaser Kazemzadeh ◽  
Ali Gorzi ◽  
Seyed Ali Hosseini ◽  
Saeid Sedaghati
Keyword(s):  
Oxidative Stress ◽  
Resistance Training ◽  
Anabolic Steroids ◽  
Kidney Tissue ◽  
The Body ◽  
Stress Factors ◽  
Male Rats ◽  
Control Group ◽  
Average Weight ◽  
Testosterone Enanthate

Introduction: Taking anabolic steroids can affect the state of oxidative stress in different tissues of the body such as kidneys. This study aimed to examine the effect of 8 weeks of resistance training with testosterone enanthate consumption on tissue oxidation indicators in male rats. Methods: In the current experimental study, 18 rats with an average weight of 250 ± 50 g were divided into 3 groups of 6 animals including: (1) control, (2) training, and (3) training + testosterone. Rats in the training and training + testosterone groups underwent resistance training for 8 weeks and 5 sessions per week. Also, rats in the training + testosterone group received 20 mg/kg of testosterone enanthate by intramuscular injection 3 days a week. The resistance training protocol consisted of climbing a 1-meter ladder 5 days a week (4 sets of 6 with a rest interval of 60 to 90 seconds) in which the weights were 60% of the rats’ body weights in the first week, and 20% of the body weight was added to the weights every week. One-way analysis of variance was used to analyze the research findings (P≤0.05). Results: Glutathione peroxidase (GPx) level in the training + testosterone group was significantly lower than the control group (P=0.007); however, there were no significant differences in malonaldehyde (MDA) (P=0.64) and superoxide dismutase (SOD) (P=0.29) levels between the research groups. Conclusion: It seems that testosterone consumption can be effective in increasing oxidative stress factors in the kidney tissue of resistance-trained rats.

scholarly journals Comparative Assessment on Mechanism Underlying Renal Toxicity of Commercial Formulation of Roundup Herbicide and Glyphosate Alone in Male Albino Rat

2018 ◽  
Vol 37 (4) ◽  
pp. 285-295 ◽  
Author(s):  
Gabriel A. Dedeke ◽  
Folarin O. Owagboriaye ◽  
Kehinde O. Ademolu ◽  
Olanrewaju O. Olujimi ◽  
Adeyinka A. Aladesida
Keyword(s):  
Oxidative Stress ◽  
Active Ingredient ◽  
High Performance ◽  
Kidney Tissue ◽  
Male Rats ◽  
Control Group ◽  
Albino Rat ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

There have been major concerns that the nephrotoxicity of commercial formulations of Roundup herbicide is due to the active ingredient glyphosate. We therefore investigated and compared the mechanisms underlining the nephrotoxicity of Roundup herbicide and glyphosate alone in rat. Fifty-six adult male rats randomized into 7 groups of 8 rats per group were exposed to Roundup formulation and glyphosate alone daily by gavage at 3.6, 50.4, and 248.4 mg/kg body weight (bw) of glyphosate concentrations for 12 weeks with distilled water administered to the control group. Kidney biomarker (serum urea and creatinine, plasma cystatin-C, and neutrophil gelatinase-associated lipocalin), oxidative stress indices in the kidney tissue, activities of kidney membrane-bound enzymes (Mg-adenosine triphosphatase [ATPase], Ca-ATPase, Na/K-ATPase, and total ATPase), and histopathological changes in the kidney were monitored. Glyphosate concentration in the kidney was quantified by high-performance liquid chromatography with ultraviolet detection. Significant ( P < 0.05) alterations in the levels of the kidney biomarker, oxidative stress markers, and membrane-bound enzymes were observed in the rats exposed to Roundup compared to the rats exposed to glyphosate alone. Rats exposed to Roundup accumulated more glyphosate residue in their kidney tissue. Severe histopathological lesions were only seen in the kidneys of rats exposed to Roundup. The nephrotoxicity observed cannot be due to the active ingredient in the Roundup formulation, as glyphosate alone has virtually no effect on the renal function of the exposed animals. Therefore, the general claim attributing nephrotoxicity of a glyphosate-based herbicide to its active ingredient should be discouraged.

scholarly journals Effect of Nandrolone Treatment with And Without Resistance Training on Superoxide Dismutase Concentration and Pathology of Kidney Tissue in Rats

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Esfandiyar Heidari ◽  
Seyed Ali Hosseini ◽  
Mohammad Ali Azarbayjani
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Resistance Training ◽  
Anabolic Steroids ◽  
Kidney Tissue ◽  
Sod Activity ◽  
Significant Difference ◽  
Stress Levels ◽  
Sh Group ◽  
Male Wistar Rats

Background: The prevalence of anabolic steroids abuse in athletes and non-athletes is associated with the risk of injury to various organs, but there are limited studies of oxidative changes in kidney tissue following nandrolone (N) administration. Objectives: the aim of this study was to investigate the effect of N treatment with and without resistance training (RT) on superoxide dismutase (SOD) concentration and tissue pathology of kidney tissue in rats. Methods: In this experimental study, 20 male Wistar rats were randomly divided into four groups of five rats including 1) control (C), 2) sham (normal saline) (Sh), 3) N, and 4) N + RT. Groups 3 and 4 received 10 mg/kg N peritoneally, and the N + RT group performed 1 m ladder climbing for eight weeks and three sessions per week. SOD levels of kidney tissue were measured by ELISA and radioimmunoassay. Hematoxylin-eosin (H&E) staining was used to evaluate oxidative stress levels in kidney tissue. One-way ANOVA with Bonferroni’s post- hoc tests were used for analysis of research findings in SPSS version 22 (P ≤ 0.05). Results: SOD levels in the C group were higher than the Sh (P = 0.001), N (P = 0.001), and N + RT (P = 0.001) groups. SOD levels were lower in the Sh group than in the N (P = 0.049) and N + RT (P = 0.001) groups. However, there was no significant difference in SOD levels in the N + RT group and N group (P = 0.28). Also, oxidative stress levels were normal in tissue studies in all groups. Conclusions: It seems that Ntreatment with and without RT reduces SOD activity in kidney tissue, but more studies are needed in this regard given the normality of tissue oxidative stress results.

scholarly journals The Effect of Superoxide Dismutase on Inhibition of Acute Kidney Injury Induced by Sepsis Based on Kidney Tissue Histology and Murine Sepsis Score

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Jufitriani Ismy ◽  
Maimun Syukri ◽  
Dessy R. Emril ◽  
Nanan Sekarwana ◽  
Jufriady Ismy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Kidney Injury ◽  
Superoxide Dismutase ◽  
Kidney Tissue ◽  
Kidney Injury ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Group I ◽  
Sepsis Score

Sepsis is one of the leading causes contributing to the incidence of acute kidney injury (AKI). Oxidative stress can be used as the main approach against sepsis-induced AKI. One of the primary antioxidants that plays a role in warding off oxidative stress is superoxide dismutase (SOD). This research aimed to observe the effect of antioxidant SOD in inhibiting sepsis in AKI based on kidney tissue histopathology. The research method was an experimental laboratory with a post-test-only control group design. Twenty-five adult male rats aged 12–16 weeks, weighing between 200 and 250 g, were randomly divided into five groups: Group I, as a positive control, where rats were injected with lipopolysaccharides (LPS); Group II, as a negative control; Group III, as treatment 1, where rats were injected with LPS and administered orally with SOD (Glisodin®) 250 IU daily; Group IV, as treatment 2, where rats were injected with LPS and administered orally with SOD (Glisodin®) 500 IU daily; and Group V, as treatment 2, where rats were injected with LPS and administered orally with SOD (Glisodin®) 1000 IU daily. Rats were administered with SOD (Glisodin®) by oral gavage with a flexible feeding tube for 16 weeks, given once daily in the morning, and then injected with LPS of 10 mg/kg body weight. Glisodin SOD had a significant effect on murine sepsis score (MSS). MSS influenced the tubular injury score linearly. We conclude that the optimal dose of SOD at 1000 IU for inhibiting sepsis-induced AKI incidence is compared to SOD at a dose of 250 and 500 IU. The antioxidant effect of SOD can prevent sepsis-induced AKI with oxidative stress events.

scholarly journals Cafeteria Diet-Induced Metabolic and Cardiovascular Changes in Rats: The Role of Piper nigrum Leaf Extract

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Dorothee L. E. Mballa ◽  
Fanta S. A. Yadang ◽  
Armelle D. Tchamgoue ◽  
Jean R. Mba ◽  
Lauve R. Y. Tchokouaha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Weight Gain ◽  
Relative Weight ◽  
The Body ◽  
Cafeteria Diet ◽  
Male Rats ◽  
Piper Nigrum ◽  
Control Group ◽  
Reference Drug ◽  
Cardiovascular Changes

Background. Cafeteria diet is known to induce excessive body fat accumulation (obesity) that could cause metabolic and cardiovascular changes and even death. The increase in prevalence over time and the failure in treatment options make obesity a real public health problem. The present study assessed the preventive effect of the hydro-ethanolic extract of the Piper nigrum leaf on the development of metabolic and cardiovascular changes in cafeteria diet fed Wistar rats. Methods. Thirty-six male rats were divided into 5 groups of 6 rats each: a normal control group (Nor.), a negative control group (Neg.), two groups administered different doses of extract in mg/kg (E250 and E500), and a group administered atorvastatin 10 mg/kg (Ator., reference drug). The animals were fed with experimental diets (standard and cafeteria) for a period of 5 weeks. Food and water intake were assessed daily, and the body weight assessed weekly. At the end of the feeding, plasma lipid profile and markers of hepatic and renal function were assessed. Furthermore, the relative weights of the adipose tissue and the organs were assessed. The liver, kidneys, and heart homogenates were assessed for markers of oxidative stress while the aorta was histopathologically examined. Results. Cafeteria diet-induced weight gain of 30% and increased triglyceride, total cholesterol, and low-density lipoprotein cholesterol level of more than 50%. Equally, an increase in the relative weight of accumulated adipose tissues of more than 90%, oxidative stress, and alteration in the organ structure were visible in cafeteria diet fed rats (Neg). Treatment with P. nigrum extract significantly prevented weight gain, dyslipidemia, oxidative stress, and alteration in the architecture of the aorta. The effect of P. nigrum extract was comparable to that of the reference drug. Conclusion. Piper nigrum leaf may prevent weight gain and possess cardioprotective activity with a strong antioxidant activity.

scholarly journals Ethanolic Extract of Moringa oleifera Leaves Influences NF-κB Signaling Pathway to Restore Kidney Tissue from Cobalt-Mediated Oxidative Injury and Inflammation in Rats

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1031 ◽  
Author(s):  
Mohamed M. Abdel-Daim ◽  
Samah R. Khalil ◽  
Ashraf Awad ◽  
Ehsan H. Abu Zeid ◽  
Reda Abd El-Aziz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Moringa Oleifera ◽  
Expression Profiles ◽  
Quantitative Polymerase Chain Reaction ◽  
Kidney Tissue ◽  
Ethanolic Extract ◽  
Male Rats ◽  
Control Group ◽  
The Impact

This study aimed to describe the protective efficacy of Moringa oleifera ethanolic extract (MOEE) against the impact of cobalt chloride (CoCl2) exposure on the rat’s kidney. Fifty male rats were assigned to five equal groups: a control group, a MOEE-administered group (400 mg/kg body weight (bw), daily via gastric tube), a CoCl2-intoxicated group (300 mg/L, daily in drinking water), a protective group, and a therapeutic co-administered group that received MOEE prior to or following and concurrently with CoCl2, respectively. The antioxidant status indices (superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)), oxidative stress markers (hydrogen peroxide (H2O2), 8-hydroxy-2-deoxyguanosine (8-OHdG), and malondialdehyde (MDA)), and inflammatory response markers (nitric oxide (NO), tumor necrosis factor (TNF-α), myeloperoxidase (MPO), and C-reactive protein (CRP)) were evaluated. The expression profiles of pro-inflammatory cytokines (nuclear factor-kappa B (NF-kB) and interleukin-6 (IL-6)) were also measured by real-time quantitative polymerase chain reaction (qRT-PCR). The results showed that CoCl2 exposure was associated with significant elevations of oxidative stress and inflammatory indices with reductions in the endogenous tissue antioxidants’ concentrations. Moreover, CoCl2 enhanced the activity of the NF-κB inflammatory-signaling pathway that plays a role in the associated inflammation of the kidney. MOEE ameliorated CoCl2-induced renal oxidative damage and inflammatory injury with the suppression of the mRNA expression pattern of pro-inflammatory cytokine-encoding genes. MOEE is more effective when it is administered with CoCl2 exposure as a prophylactic regimen. In conclusion, MOEE administration exhibited protective effects in counteracting CoCl2-induced renal injury in rats.

Influence of selenium and/or magnesium on alleviation alcohol induced oxidative stress in rats, normalization function of liver and changes in serum lipid parameters

2011 ◽  
Vol 30 (11) ◽  
pp. 1811-1827 ◽  
Author(s):  
Iwona Markiewicz-Górka ◽  
Marcin Zawadzki ◽  
Lidia Januszewska ◽  
Katarzyna Hombek-Urban ◽  
Krystyna Pawlas
Keyword(s):  
Oxidative Stress ◽  
Liver Function ◽  
Total Cholesterol ◽  
Cholesterol Metabolism ◽  
Body Weight Gain ◽  
Total Antioxidant Status ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Histopathological Changes

The aim of this study was to evaluate the attenuating effect of given selenium and/or magnesium on ethanol-induced oxidative stress, disturbances of liver function and cholesterol metabolism. Forty male rats were divided into five groups: C – control, Et – intoxicated with alcohol (15% solution in drinking water), Et + Mg, Et + Se, Et + Mg + Se – intoxicated with alcohol and supplemented with selenium (0.4 mg Se/l water), magnesium (100 mg Mg/l water) and combination of Se and Mg, respectively. The experiment was carried out over the 3 months. The results show that the chronic ingestion of alcohol induces lipid peroxidation and histopathological changes in liver. Supplementation with magnesium only partially alleviates oxidative stress and damages in this tissue. The both selenium alone and combination of magnesium and selenium significantly elevated total antioxidant status (TAS) in serum, activity of glutathione peroxidase and ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) in liver and retarded oxidative stress and histopathological changes in this tissue. Chronic administration of ethanol (alone and with magnesium) resulted in significant decrease in the serum total cholesterol and retardation in the body weight gain in comparison with the control group. In the groups supplemented with selenium and selenium and magnesium simultaneously, concentration of total cholesterol in serum and body gains was similar to the control group. Supplementation of Se or selenium and magnesium simultaneously significantly enhances antioxidant defence and is more effective against alcohol-induced oxidative stress, disturbance of liver function and cholesterol metabolism than the separate use of magnesium.

Effects of quercetin on methotrexate-induced nephrotoxicity in rats

2016 ◽  
Vol 36 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Yasemin Yuksel ◽  
Ramazan Yuksel ◽  
Murat Yagmurca ◽  
Hacer Haltas ◽  
Husamettin Erdamar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Structural Changes ◽  
Caspase 3 ◽  
Periodic Acid ◽  
Antioxidant Properties ◽  
Kidney Tissue ◽  
Male Rats ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Control Group ◽  
Therapeutic Effects

Objective: This experimental study was conducted to elucidate the possible protective/therapeutic effects of quercetin against methotrexate (Mtx)-induced kidney toxicity with biochemical and histopathological studies. Methods: Twenty-four adult male rats were randomly divided into four groups, as follows: control group (saline intraperitoneally (i.p.), 9 days), Mtx group (20 mg/kg i.p., single dose), Mtx + quercetin group (50 mg/kg quercetin was orally administered 2 days before and 6 days after Mtx administration) and only quercetin group (50 mg/kg oral, 9 days). Structural changes were evaluated by hematoxylin–eosin and periodic acid–Schiff stainings. Apoptotic changes were investigated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay and caspase-3 antibody. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels were measured in tissue and plasma samples. Results: Mtx compared with the control group, there was significant increase in nephrotoxic tissue damage findings, in addition to apoptotic index (APOI) and caspase-3 expression ( p < 0.05). Mtx + quercetin group revealed significantly lower histopathological damage and APOI and caspase-3 expression decreased when compared to Mtx group. MDA levels were increased in Mtx group compared to others, and by the use of quercetin, this increase was significantly reduced. SOD levels were higher in Mtx group than others. This increase was evaluated as a relative increase arising from oxidative damage caused by Mtx. Conclusion: As a result, Mtx administration may involve oxidative stress by causing structural and functional damage in kidney tissue in rats. Quercetin reduced the Mtx-induced oxidative stress through its antioxidant properties and so quercetin may be promising to alleviate Mtx-induced renal toxicity.

scholarly journals The Effect of Mangosteen Rind Extract (Garcinia mangostana L.) Toward Stress Oxidative Parameter, Leukocytes, Leukocytes Type Counts on Male Rats (Rattus norvegicus) with Excessive Physical Activity

2020 ◽  
Vol 8 (A) ◽  
pp. 904-909
Author(s):  
Rika Nailuvar Sinaga ◽  
Zulaini Zulaini
Keyword(s):  
Oxidative Stress ◽  
Physical Activity ◽  
Rattus Norvegicus ◽  
Leukocyte Count ◽  
The Body ◽  
Male Rats ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Average Percentage ◽  
White Rats

BACKGROUND: Excessive and heavy physical activity could lead to the oxidative stress in the body. This condition can affect several blood parameters such as malondialdehyde (MDA), glutathione peroxidase (GPx), leukocytes, and its type counts. One way to overcome the effects of oxidative stress due to strenuous physical activity is by giving natural antioxidants. Mangosteen rind (Garcinia mangostana L.) contains considerable antioxidants, namely, xanthones and procyanidin. AIM: This research aimed to investigate the effect of mangosteen rind extract (MRE) on oxidative stress parameters, leukocyte count, and leukocyte count in male rats (Rattus norvegicus) that received excessive physical activity. METHODS: This study was purely experimental research with a post-test only control group design, using 29 white rats (R. norvegicus) as experimental animals, which were divided into four groups. The first group (P1) received distilled water and the other group received MRE at a dose of 50 mg/KgBW (Body Weight) (P2), 100 mg/KgBW (P3), and 200 mg/KgBW (P4) for 3 weeks, respectively. Swimming as a physical activity was carried out 3 times a week in all groups. Examination of leukocyte count and species was carried out on the last day of treatment after the rats swam to fatigue. RESULTS: MDA levels did not decrease significantly while GPx activity increased significantly. Moreover, the average leukocytes and lymphocytes showed a decrease but not significant. A significant decrease in the percentage of neutrophils and basophils was obtained by P4 group compared to others. In addition, a significant decrease in the average percentage of eosinophils for P3 and P4 groups was obtained. Furthermore, P3 and P4 group exhibit significant increase of the average percentage of monocytes. CONCLUSION: MRE as an antioxidant affects the levels of neutrophils, basophils, eosinophils, and monocytes but not in MDA, GPx, leukocytes, and lymphocytes.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Diabetes Type 2 ◽  
Male Rats ◽  
Control Group ◽  
Standard Drug ◽  
High Fructose ◽  
Group A ◽  
Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

Sign in / Sign up

Export Citation Format

Share Document