scholarly journals Pemanfaatan ekstrak Wortel (Daucus carrota L.) sebagai imunostimulan pada ikan Kakap Putih (Lates calcarifer)

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Muhammad Aris ◽  
. Juharni ◽  
Taufiq Abdullah

This study aims to determine the effect of carrot extract on the phagocyte activity of Vibrio alginolyticus in white snapper. This study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used respectively as follows, treatment A: 0 µl extract/individu, B: 100 µl extract/individu, C: 150 μl extract/individu, and D: 200 μl extract/individu. The average value of phagocytic activity tended to increase, namely treatment A 8.67%; treatment B 9.33%; treatment C 11.00%; and treatment D 14.00%. Carrot extract can increase phagocytic activity of white snapper. An effective dose that can increase the immune response of white snapper was 200 µl extract/individu of fish.

2020 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Mihwan Sataral ◽  
Hendra Heri Robika ◽  
Zaedar A Masese

Salah satu faktor yang menyebabkan penurunan produksi kakao yaitu serangan hama penggerek buah kakao. Semut hitam (Dolichoderus thoracicus) diketahui sebagai agen hayati pengendali hama penggerek buah kakao. Penelitian ini bertujuan untuk menghitung kemampuan memangsa semut hitam pada larva penggerek buah kakao, dan menghitung persentase larva penggerek buah kakao yang dimangsa serta mengukur potensi semut hitam sebagai agen hayati penggerek buah kakao. Penelitian ini di laksanakan di Desa Jaya Makmur Kecamatan Nuhon Kabupaten Banggai. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 ulangan, dengan masing-masing perlakuan sebagai berikut : P1 (20 larva:10 predator), P2 (20 larva:15 predator), P3 (20 larva:20 predator), P4 (20 larva:25 predator), P5 (20 larva:30 predator), P6 (20 larva:35 predator). Hasil penelitian menunjukkan bahwa perlakuan semut hitam pada larva penggerek buah kakaoSalah satu faktor yang menyebabkan penurunan produksi kakao yaitu serangan hama penggerek buah kakao. Semut hitam (Dolichoderus thoracicus) diketahui sebagai agen hayati pengendali hama penggerek buah kakao. Penelitian ini bertujuan untuk menghitung kemampuan memangsa semut hitam pada larva penggerek buah kakao, dan menghitung persentase larva penggerek buah kakao yang dimangsa serta mengukur potensi semut hitam sebagai agen hayati penggerek buah kakao. Penelitian ini di laksanakan di Desa Jaya Makmur Kecamatan Nuhon Kabupaten Banggai. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 ulangan, dengan masing-masing perlakuan sebagai berikut : P1 (20 larva:10 predator), P2 (20 larva:15 predator), P3 (20 larva:20 predator), P4 (20 larva:25 predator), P5 (20 larva:30 predator), P6 (20 larva:35 predator). Hasil penelitian menunjukkan bahwa perlakuan semut hitam pada larva penggerek buah kakao (Conopomorpha cramerella) berpengaruh sangat nyata terhadap larva penggerek buah kakao. Jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 (20 larva:35 predator) dua minggu setelah aplikasi dengan nilai rata-rata 16,3 kemudian satu minggu setelah aplikasi dengan nilai rata-rata 14,3. Persentase jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 yaitu 81% pada saat dua minggu setelah aplikasi dan 0,71% pada saat satu minggu setelah aplikasi. (Conopomorpha cramerella) berpengaruh sangat nyata terhadap larva penggerek buah kakao. Jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 (20 larva:35 predator) dua minggu setelah aplikasi dengan nilai rata-rata 16,3 kemudian satu minggu setelah aplikasi dengan nilai rata-rata 14,3. Persentase jumlah larva penggerek yang dimangsa tertinggi pada perlakuan P6 yaitu 81% pada saat dua minggu setelah aplikasi dan 0,71% pada saat satu minggu setelah aplikasi. One of the factors that caused the decline in cocoa production was the attack of cocoa pod borer. Black ants (Dolichoderus thoracicus) are known to be biological agents to control cocoa pod borer. This study aims to calculated the ability to prey on black ants in the cocoa pod borer larvae, the percentage of cocoa pod borer larvae that are eaten and to measured the potential of black ants as biological agents of cocoa pod borer. This research was conducted in Jaya Makmur Village, Nuhon District, Banggai Regency. The research used a completely randomized design consisted of 6 treatments and 3 replications, with each treatment as follows : P1 (20 larvae: 10 predators), P2 (20 larvae: 15 predators), P3 (20 larvae: 20 predators), P4 (20 larvae: 25 predators), P5 (20 larvae: 30 predators), and P6 (20 larvae : 35 predators). The results showed that the treatment of black ants on cocoa pod borer larvae (Conopomorpha cramerella) had a very significant effected on the cocoa pod borer larvae. The highest number of dead borer larvae was in the P6 treatment (20 larvae: 35 predators) two weeks after application with an average value of 16.3 then one week after application with an average value of 14.3. The highest percentage of borer larvae preyed on the P6 treatment was 81% at two weeks after application and 0.71% at one week after application.


2021 ◽  
Vol 11 (1) ◽  
pp. 39-47
Author(s):  
Bagus Uda Palgunadi ◽  
Ratna Widyawati ◽  
Dian Ayu Kartika Sari ◽  
Halimah Tusadiah

The purpose of this research aims to determine the effect of adding enzymes (amylase, protease, xylanase) in feed on egg weight and egg yolk diameter in mixed ducks. The experimental animals used were 24 laying ducks. The design used was a completely randomized design (CRD) with 4 treatments and 6 replications. The four treatments were P0 (Control), P1 (6 ducks with Avizym1502®5gram / kg), P2 (6 ducks with Avizym1502® 10gram / kg), and P3 (6 ducks given Avizym1502). ® 15gram / kg). This treatment is carried out once a day for 9 weeks. Before the treatment, the ducks were adapted for a week. After that, the initial egg weight and yolk diameter were measured at week 0, week 6, and week 9. Data obtained from the results of weighing eggs found an average value of P0 (59.1667 ± 3.9200a) g, P1 5gram / kg (56.1667 ± 2.9268a) g, P2 10gram / kg (60.1667 ± 4.9966a) g, and P3 15gram / kg (67.6667 ± 2.7325ᵇ) g. The results of data from examining the diameter of the egg yolk found an average value of P0 (45.03 ± 1.2956a) mm, P1 5gram / kg (44.35 ± 0.7969a) mm, P2 10gram / kg (45.75 ± 1.5694a) mm, and P3 15gram / kg (48.83 ± 0.7394ᵇ) mm. The data obtained were then analyzed using the ANOVA test which statistically showed that there was a significant difference (P <0.05) between egg weight and yolk diameter in mixed ducks treated with enzymes (amylase, protease, xylanase) in the feed.


2021 ◽  
Vol 10 (3) ◽  
pp. 380
Author(s):  
Linayati Linayati ◽  
Bramantiar Rizkyansyah ◽  
Tri Yusufi Mardiana ◽  
Muhammad Zulkham Yahya

The purpose of this study was to determine the effect of adding honey to the feed for growth and the best dosage for the growth of white snapper seeds.The white snapper seeds used have an average weight of ± 2.5 g / fish.This study used an experimental method with a Completely Randomized Design (CRD).The experiment was carried out in 5 (five) treatments and each treatment had 3 (three) repetitions.The treatment consisted of adding honey to the feed with a treatment dose of (A) 0 ml / kg of feed, (B) 50 ml / kg of feed, (C) 100 ml / kg of feed, (D) 150 ml / kg of feed and (E) 200 ml / kg of feed.The results indicated that the addition of honey to the feed had a significant effect on the growth of white snapper seeds.The best dose of honey is at a dose of 200 ml / kg which has the best effect on fish growth.The condition of water quality during the study was in a suitable range for the life of white snapper seeds.


2021 ◽  
Vol 3 (2) ◽  
pp. 76-90
Author(s):  
Fransiskus X. Manehat ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk

This study aims to determine the quality of Bali cattle spermatozoa diluted using sugarcane juice-egg yolk. This research was carried out in the stables and Laboratory of the Faculty of Agriculture, University of Timor in August-September 2020. Semen was collected from an adult male bali cattle, aged ± 4.5 years in healthy condition. The method used in this study is an experimental method using a completely randomized design consisting of 4 treatments and 5 replications. Each treatment was T1: 0.075 ml semen + 0.3 ml of egg yolk sugarcane juice diluent and stored for 24 hours, T2: semen 0.075 ml + 0.3 ml of diluent for egg yolk cane juice and stored for 48 hours, T3: semen 0.075 ml + 0.3 ml of diluent for egg yolk sugarcane juice and stored for 72 hours, T4: semen 0.075 ml + 0 ,3 ml of egg yolk sugarcane juice diluent and stored for 96 hours. The variables measured were individual motility, viability, spermatozoa abnormalities, and semen pH. The results showed that the T4 treatment on a shelf life of 96 hours still showed a good average value with individual spermatozoa motility values of 45%, spermatozoa viability of 77.3%, spermatozoa abnormalities of 12.5% and semen pH 6.6. It can be concluded that the use of cane juice-egg yolk diluent has a positive impact because it is able to maintain the viability of spermatozoa for 96 hours.    


2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Frangki Sigaha ◽  
Ellen J Saleh ◽  
Srisukmawati Zainudin

This study aims to determine the evaluation of the percentage of super native chicken carcasses by providing fermented corn straw. The research design used in the study was a Completely Randomized Design consisting of 5 treatments and 4 replications namely P0 (ration without treatment), P1 (ration containing 5% fermented corn straw), P2 (ration containing 10% fermented corn straw), P3 (ration ration containing 15% fermented corn straw) and P4 (ration containing 20% fermented corn straw). The data obtained were then analyzed using the Analysis of variance (ANOVA) and if the results were influential then proceed with the Duncan Distance test. The results showed that the treatment had no significant effect (P> 0.05) on the variables of life weight, carcass weight and percentage of carcass cut. The highest living weight was found in the P0 treatment (without using fermented corn straw) with the acquisition of an average value of 850.75 gr / head and produced the highest carcass weight of 197.25 gr. The highest percentage of carcass cuts was obtained at P3 treatment (15% fermented corn straw) with an average value of 23.88%.


2006 ◽  
Vol 8 (1) ◽  
pp. 25
Author(s):  
Des Roza ◽  
Fris Johnny ◽  
Tridjoko Tridjoko

An experiment to evaluate the effectiveness immunostimulant and bacterin on humpback grouper have been conducted at the Disease Laboratory of Research Institute for Mariculture, Gondol, Bali. The experiment was designed in completely randomized design with four treatments in duplicates. Two hundred of humpback grouper juveniles (15-18 cm of total length, 55-65 g of body weight) were injected intraperitoneally with (A) bacterine at 107 cfu/kg body weight (BW), (B) peptidoglycan at 100 mg/kg BW, and (C) immuno star at 100 ml/kg BW, (D) control. The fish were then challenged with VNN by intramuscular injection at 10 days post treatment. Results showed that survival rates of juvenile after challenged with VNN were 60.00% (B & C), 53.34% (A), and 1.67% (control). Parameters of non-spesific immune respons showed that phagocytic activities were 17.56% (B), 17.55% (C), 13.11% (A), and 9.33% (control). In addition, lisozyme activities were 1.64 cm (B), 1.58 cm (C), 1.55 cm (A), and 1.46 cm (control). Immunostimulant and bacterin stimulated non-specific immune response, and increased survival rate of humpback grouper juvenile.


2019 ◽  
Vol 7 (1) ◽  
pp. 71
Author(s):  
Mohamad Yusuf ◽  
Gunanti Mahasri ◽  
Mufasirin Mufasirin

Abstract Myxobolus is one of parasites on koi fish that belongs to a class myxosporea that can infect and systemic and can cause harm to the fish farming. Vaccination is an attempt to cause-specific endurance through vaccination. Observations differential leukocytes and increased optical density values can be used to determine the effectiveness of the vaccine is given. This study aims to analyze the immune response koi fish vaccinated with Myxobolus koi spores whole protein for vaccine development myxobolusis in koi fish. The method used in this study is Completely Randomized Design with 4 treatments and 5 replications. The results showed that a change in the total number and types of leukocytes that can be used as indicators of the presence of certain infectious diseases that occure in fish. The highest value of lymphocytes in treatment B, monocytes highest in treatment D, neutrophils on treatment D, eosinophils on treatment A and basophils highest in treatment A. The observation of the highest optical density value in treatment B (fish vaccinated and infected 80 M. koi spores / tail) of 0.593 at day 30, while the lowest in treatment D (fish are not vaccinated but diinveksi 80 M. koi spores / tail) of 0,064 in 30 days


2019 ◽  
Vol 4 (1) ◽  
Author(s):  
Muslim Muslim ◽  
Ade Dwi Sasanti ◽  
Apriana Apriana

The aim of this research was to determine the best immertion duration to increase growth rate of snakehead fish larva. The research was conducted in Fish Breeding unit Batanghari Sembilan Indralaya, Kabupaten Ogan Ilir. This research used a Completely Randomized Design (CRD), with five treatments (0, 12, 24, 36, and 48 hours of immertion), with three replications. The parameters observed were growth, survival rate and water quality. The results of  this research show that the highest growth rate found in the 24 hours of treatment  with the average value of 0.17 g of weight and increase in length of 1.90 cm. The best survival contained in treatment of immersion for 36 hours with the average value of 71.67%.


bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Darmawan Jamaluddin ◽  
Nurhaeda Nurhaeda ◽  
Rasbawati Rasbawati

Abstract. The purpose of this study was to determine the increase in protein content and decrease in crude fiber in complete feed silage based on rice straw and lamtoro leaves as ruminant feed. This study used a completely randomized design (CRD) with 4 treatment levels and 3 replications namely J0 = 100% rice straw (control), J1 = 60% rice straw and lamtoro leaves 40% J2 = 70% rice straw and lamtoro leaves 30% J3 = 80% rice straw and lamtoro leaves 20%. The results showed that the combination of rice straw and lamtoro leaves had a very significant effect on the value of crude protein and crude fiber in complete feed silage. The highest average to the lowest protein content was J1 (11.45), J2 is equal to (9.50), J3 is equal to (8.44), and J0 is equal to (6.60). The average value of the highest crude fiber content to the lowest is (35.19), J3 is equal to (33.82), J2 is equal to (32.35), and J1is equal to (30.55). From the results of the study it is known that the best treatment that can be recommended is J1 treatment with a combination of 60% rice straw and lamtoro leaves 40%. Keywords: coarse protein, coarse fiber, complete feed silage, lamtoro leaves and rice straw.


Author(s):  
Yuniel Méndez-Martínez ◽  
Ginger Pacheco-Morales ◽  
Karla Del Barco-Ibarra ◽  
Yenny Torres-Navarrete ◽  
Martha Hernández-Vergara

The use of biostimulants in fish diets is a promising strategy to reduce the use of antibiotics, enhance the biochemical and immune response, which contributes to improving productive yields and reducing economic losses. The biochemical and immune response was evaluated in juvenile red tilapia (Oreochromis mossambicus × O. niloticus), with different levels: 0 (control), 1, 2, 3, 4 and 5% chitosan in diet. 270 animals (7.53 ± 0.50 g of initial weight), distributed in a completely randomized design of 6 treatments with 3 replicates and 45 tilapia.treatment-1, were sown in 18 tanks with 90 L of water in a closed system. The juveniles were fed for 55 days with the experimental diets and at the end of the bioassay the tissue and blood plasma samples were taken. Differences were found (p<0.05) in the variables evaluated responses, where the content of lipids, triglycerides, cholesterol, carbohydrates and glucose showed higher levels (p<0.05) at lower chitosan concentrations. However, for protein content, a better response (p<0.05) was found at higher levels of chitosan in the diet. Regarding dry matter and ash, no differences were found (p˃0.05). While superoxide dismutase and leukocytes were found in higher concentration (p<0.05) when supplemented with chitosan by 3 and 4%. It was concluded that the juvenile biochemical and immune response of red tilapia was favored with dietary chitosan supplementation.


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