scholarly journals Sterility Test of Syringes As A Pharmaceutical Preparation That Obtained From Pasar Pramuka

2021 ◽  
Vol 12 (2) ◽  
pp. 186-198
Author(s):  
Junie Suriawati ◽  
Reni Anggraini ◽  
Siti Rahayu Rachmawati ◽  
Yulis Adriana
Keyword(s):  
Escherichia Coli ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Salmonella Typhi ◽  
High Demand ◽  
Clostridium Sporogenes ◽  
Selective Media ◽  
Aspergillus Brasiliensis ◽  
Bacillus Subtilis Atcc ◽  
Pathogenic Microbes

Syringes are one of the pharmaceutical preparations that are in high demand. In healthcare institutions, syringes are used to aid in patient care and examination. Pharmaceutical preparations, such as various syringes, are widely available in the Pasar Pramuka. Syringes must be free from microbes and used syringes should not be reused. Microbiological sterility tests can be performed on a syringe to determine whether it is sterile or not. The purpose of the study is to test the sterility of syringes obtained from the Pasar Pramuka. A random sample of a syringe is being used in the study as an experimental method. The syringes were isolated and incubated for 14 days in Fluid Thioglycollate Medium (FTM) and Trypticase Soy Broth (TSB) at 30-35 oC and 20-25 oC, respectively, with frequent observations. If FTM and TSB media were turbid, then isolated into selective media based on their microbe as controls, namely Clostridium sporogenes ATCC 19404, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, Salmonella typhi. The results showed that the B syringe was turbid on FTM media and did not contain pathogenic microbes after being identified on selective media, as in controls. The A and C syringes on TSB media were turbid, and after identification on selective media, Candida albicans and Aspergillus brasiliensis were found. In conclusion, the A, B, and C syringes are not steril.

scholarly journals Design, Synthesis, and Antimicrobial Evaluation of New Annelated Pyrimido[2,1-c][1,2,4]triazolo[3,4-f][1,2,4]triazines

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1339 ◽  
Author(s):  
Islam H. El Azab ◽  
Nadia A.A. Elkanzi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Positive Control ◽  
Design Synthesis ◽  
Aspergillus Brasiliensis ◽  
In Vitro Investigation ◽  
Bacillus Subtilis Atcc ◽  
Antimicrobial Evaluation

A series of 34 new pyrimido[2,1-c][1,2,4]triazine-3,4-diones were synthesized and fully characterized using IR, NMR, MS, and microanalytical analysis. In vitro investigation of 12 compounds of this series revealed promising antimicrobial activity of the conjugates 15a and 15f–j that were tagged with electron-withdrawing groups, with sensitivities ranging from 77% to as high as 100% of the positive control. The investigation of antimicrobial activity included Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6535, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 8739 (EC), and fungal strains Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404.

scholarly journals Identification and antibiogram study of bacterial species isolated from milk samples of different locations in Bangladesh

2016 ◽  
Vol 1 (3) ◽  
pp. 457-462 ◽  
Author(s):  
Md Nuruzzaman Munsi ◽  
Nathu Ram Sarker ◽  
Razia Khatun ◽  
Mohammed Khorshed Alam
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Public Health Importance ◽  
Milk Samples ◽  
First Choice

Cow’s milk containing pathogenic bacteria is an important threat to the consumers. The objectives of the present study were to identify the bacterial agents of public health importance in milk samples (n=35) of different locations and to determine their sensitivity to different antibiotics. The milk samples were collected and transported aseptically and subsequently allowed for culture in bacteriological media, Gram’s staining and biochemical tests for the identification of bacterial species. The bacteria identified were Staphylococcus aureus, Escherichia coli and Salmonella typhi, and their prevalence, in case of vendor milk specimens (n=28), were 96.43%, 53.57% and 35.71% respectively, and of brand milk specimens (n=7), were 42.86 %, 28.57% and 0%, respectively. This suggests that cautionary measures should be taken for quality milk production and consumption. The antibiotic sensitivity test was done by disc diffusion method and the average inhibition zones, in case of Staphylococcus aureus, were 32 mm for oxytetracycline, 26 mm for amoxicillin, 35 mm for ciprofloxacin, 27 mm for cefotaxime, 30 mm for ceftriaxone, 30 mm for azithromycin, and 26 mm for erythromycin; in case of Escherichia coli, were 5 mm for oxytetracycline, 9 mm for amoxicillin, 22 mm for ciprofloxacin, 30 mm for cefotaxime, 31 mm for ceftriaxone, 15 mm for azithromycin, and 0 mm for erythromycin; in case of Salmonella typhi., were 25 mm for oxytetracycline, 24 mm for amoxicillin, 38 mm for ciprofloxacin, 31 mm for cefotaxime, 34 mm for ceftriaxone, 24 mm for azithromycin, and 0 mm for erythromycin. Therefore, ciprofloxacin and ceftriaxone may be the antibiotics of first choice, and cefotaxime and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.Asian J. Med. Biol. Res. December 2015, 1(3): 457-462

scholarly journals Níveis de sensibilidade de enterobactérias, em particular Salmonella typhi, a rifamicina S.V.

1969 ◽  
Vol 3 (2) ◽  
pp. 87-93
Author(s):  
Ivone R. Suassuna ◽  
I. Suassuna ◽  
C. E. de V. Serpa
Keyword(s):  
Escherichia Coli ◽  
Proteus Mirabilis ◽  
Salmonella Typhi ◽  
E Coli

Em face à predominante eliminação biliar da rifamicina S.V. atingindo concentrações muitas vêzes superiores aos níveis séricos obtidos com as doses terapêuticas, e pelo possível interêsse dessa verificação para o tratamento dos portadores biliares crônicos de Salmonella typhi determinou-se a concentração mínima inibitória de 165 estirpes de enterobactérias, incluindo 77 amostras de S. typhi. Foi verificado que a maioria das cepas de Escherichia coli, Shigella e Proteus mirabilis correspondiam a uma concentração inibitória mínima entre 33 a 65 μg/ml. Entre 65 e 128 μg/ml foram determinadas as concetrações inibitórias mínimas da maioria das outras espécies de Proteus, de Providencia e de Klebsiella. Para Salmonella e Enterobacter o limite mínino de sensibilidade foi, em regra, igual ou superior a 128 μg/ml. Diferenças mais acentuadas de comportamento entre as enterobactérias foram observadas quanto à ação bactericida da rifamicidas S.V. De uma maneira geral, para E. coli e Shigella, as concentrações inibitórias mínimas já referidas. Para as espécies de Proteus e Providencia houve variação maior de comportamento, mas tendência a que o efeito bactericidas fôsse encontrado em concentrações que correspondiam a 4 vêzes as bacteriostáticas para as mesmas espécies. Finalmente, de modo pouco feliz para os propósitos visados, em Salmonella, com a inclusão de S. typhi, não foi atingido um efeito bactericida, com as mais altas concentrações usadas as quais corresponderam em média a 6 vêzes as concentrações bacteriostáticas para esse gênero.

scholarly journals Stability indicating HPLC-Fluorescence detection method for the simultaneous determination of linagliptin and empagliflozin in their combined pharmaceutical preparation

2021 ◽  
Vol 12 (2) ◽  
pp. 168-178
Author(s):  
Mohamed Rizk ◽  
Ali Kamal Attia ◽  
Heba Yosry Mohamed ◽  
Mona Elshahed
Keyword(s):  
Fluorescence Detection ◽  
Mobile Phase ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Significant Difference

A sensitive, accurate, and precise liquid chromatographic method has been developed and validated for the determination of Linagliptin (LNG) and Empagliflozin (EMP) in their combined tablets. Chromatographic separation was carried out on ODS-3 Inertsil® C18 column (150×4.6 mm, 5 µm). The mobile phase A (consisting of 0.30% Triethyl amine buffer (TEA) at pH = 4.5, adjusted using ortho-phosphoric acid); the mobile phase B (consisting of acetonitrile) was pumped through the column whose temperature was maintained at 40 °C, with a flow rate 1.7 mL/min, using gradient elution from 0-3 min A:B (75:25, v:v), then from 3-6 min the ratio changed to be A:B (60:40, v:v). Fluorescence detection (FLD) was performed at 410 nm after excitation at 239 nm. Acceptable linearity, accuracy and precision values of the proposed method were found over the concentration ranges of 0.5-15 µg/mL for LNG and 1.0-30 µg/mL for EMP with correlation coefficients of 0.9997 and 0.9998 in the case of LNG and EMP, respectively. The recoveries and relative standard deviations percentages were found in the following ranges: 98.56-101.85 and 0.53-1.52% for LNG and 98.00-101.95 and 0.31-1.05% for EMP. The detection and quantification limits were 0.15 and 0.45 µg/mL for LNG and 0.22 and 0.67 µg/mL for EMP. The optimized method was validated and proved to be specific, robust, accurate and reliable for the determination of the drugs in pure form or in their combined pharmaceutical preparations. No significant difference was found regarding accuracy and precision upon statistical comparison between the obtained results of the proposed method and those of the reported method. Furthermore, the proposed method is proved to be a stability-indicating assay after exposure of the studied drugs to variable forced degradation parameters, such as acidic, alkaline and oxidative conditions, according to the recommendations of the International Conference on Harmonization guidelines. The simplicity and selectivity of the proposed method allows its use in quality control laboratories.

scholarly journals Anthill Inhibiting Bacteria, a Promising Source of Bio Efficacy

2020 ◽  
pp. 14-21
Author(s):  
A. A. Katun ◽  
A. R. Abdulmumin ◽  
M. U. Yahaya ◽  
N. K. Habeeb ◽  
A. Bala
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Typhi ◽  
Biologically Active ◽  
Bacterial Strains ◽  
Active Metabolites ◽  
Bacillus Lentus ◽  
Total Prevalence ◽  
Promising Source ◽  
And Staphylococcus Aureus

The investigation into soil bacteria has been widely studied and becoming increasingly appreciated as an exceptional reservoir of unique naturally occurring biologically active metabolites with pharmaceutical applications. This article aimed to isolate, identify and biochemically characterize antibiotic-producing bacteria from anthill soils in the permanent site of Ibrahim Badamasi Babangida University, Lapai (IBBUL), Niger State, Nigeria. The sum of ten samples were collected from five sampling sites, the sampling was done in threefold (morning, noon and evening) and analyzed adopting standard microbiological protocols. The obtained result revealed that the total bacteria count in the morning ranges from 2.1×107 cfu/mL to 1.4×106 cfu/mL, noon count ranges from 3.1×107 to 2.6×106 cfu/mL while evening count was in the range of 2.1×107 cfu/mL to 1.7×106 cfu/mL. A total number of five (5) bacteria were isolated as Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Bacillus lentus and Micrococcus reseus. The total prevalence of the bacterial isolates in the morning, noon and evening were calculated as B. subtilis (109.08%), S. epidermidis (36.36%), M. reseus (36.36%), B. lentus (63.63%), and S. aureus (54.54%) respectively. These isolates were further assayed against Escherichia coli, Salmonella typhi, Klebsiella sp. and Staphylococcus aureus. The antibacterial outcome showed that two (2) (40%) anthill isolates exhibited antibacterial activity against three (3) tested bacteria (Escherichia coli, Salmonella typhi and Staphylococcus aureus). This research study has showcased that the production of inhibitory substances are common among some of the bacterial strains isolated from anthills.

scholarly journals Bacterial aerobic Bacteraemia at AL- Kindi Teaching Hospital 2007-2009: Etiology diversity, Clinical features, and Outcome.

2012 ◽  
Vol 6 (1) ◽  
pp. 3-9
Author(s):  
Suzan I. Majeed ◽  
Khawlah J. Khalaf ◽  
Mazin K. Ameen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Clinical Features ◽  
Teaching Hospital ◽  
Blood Stream ◽  
Salmonella Typhi ◽  
Blood Stream Infections ◽  
Blood Samples

Data regarding the incidence, etiology and outcome of Bacteraemia taken from AL-Kindi hospital were collected, and compared the situation between the three years: 2007, 2008, 2009. A total number of blood samples was (505), from this number only (70) (13.8%) is positive. The rate of positivity was significant greater in 2009 (17.7%) than 2008 (11.8%) and 2007 (9.1%). The calculated incidence of significant episodes of bacteraemia was 12.6% .The five most commonly isolated microorganisms were: Salmonella typhi30(46.8%), Staphylococcus aureus 12 (18.7%), Escherichia coli 10 (15.6%),Klebsiella 4(6.2%) and Enterobacter sp.3(4.6%) varied throughout this period, significant increase was noted in 2009. The acquisition of blood stream infections was nosocomial in 8.5% of cases.60% of Salmonella typhi were Amoxicillin resistant. Also Staph.aureus isolates, 58%were resistant to Amoxicillin and Erythromycin. Cephalexin-resistant E.coli in70% of the isolates and Gentamycin-resistant Klebsiella occurred in 75% of the isolates. The incidence of BSI did not differ significantly from other reported studies.

scholarly journals Evaluación de sales de cura (nitrito) y de los tratamientos físico-químico en la conservación de carne de concha Anadara tuberculosa; y su análisis microbiológico.

AKADEMOS ◽  
2017 ◽  
pp. 89-101
Author(s):  
Guillermo Antonio Bonilla Henríquez
Keyword(s):  
Escherichia Coli ◽  
Vibrio Cholerae ◽  
Salmonella Typhi ◽  
Para Determinar

Durante la investigación se midió el efecto del uso de sales de cura (nitrito a 200 ppm) y un tratamiento térmico a 70ºC por 3 minutos y 80ºC por 2 minutos para determinar la posibilidad de conservar las propiedades organolépticas y prolongas la vida de anaquel.Las pruebas microbiológicas presentaron resultados negativos en los análisis de Recuento total de bacterias, Coliformes, Coliformes fecales así como también respuestas negativas en los patógenos: Escherichia Coli, Salmonella typhi y Vibrio cholerae, indicando esto que los productos tratados no representan un peligro para la saludNo así las muestras que no fueron tratadas con ninguno de los métodos explicados donde su número más probable de bacterias fue de más de 1100 NMP/ml también un Recuento total de bacterias de 9.9 X10 4 UFC/ml, bacterias Coliformes 7.9X10 4 UFC/ml y Coliformes fecales 2.9X10 4 UFC/ml (Enterobacterias aerogenes) demostrando que una muestra “al fresco” de conchas representa un peligro para el consumidor. El análisis sensorial determinó que la Hipótesis Nula fue rechazada ya que el F Observado es mayor que el F Crítico por que este se encuentra en la zona de rechazo a un nivel de significancia del 5%. Esto demuestra que los tratamientos inciden de diferente manera sobre las muestras afectando sus propiedades sensoriales.AKADEMOS, Año 10, Vol. 2, No 27, Julio Diciembre 2016: 89-101

scholarly journals 1439. High Rates of Drug Resistance in Escherichia coli from a Pilot Antimicrobial Resistance Surveillance System in Cambodia

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S723-S724
Author(s):  
Vandarith Nov ◽  
Darapheak Chau ◽  
Chhorvann Chhea
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Blood Culture ◽  
Klebsiella Pneumoniae ◽  
Surveillance System ◽  
Methicillin Resistance ◽  
Salmonella Typhi ◽  
Blood Samples

Abstract Background Antimicrobial resistance (AMR) is a major and growing global public health problem. The Cambodia Ministry of Health established a pilot laboratory-based AMR surveillance system for blood specimens in 2017. The objective of this study is to characterize AMR among pathogenic isolates from blood samples. Methods A retrospective analysis was performed using one year of data from a pilot AMR Surveillance system in Cambodia. Four blood culture isolate pathogens were included: Escherichia coli, Klebsiella pneumoniae, Salmonella Typhi /Salmonella Paratyphi A and Staphylococcus aureus. Blood culture isolates that were referred from eleven sentinel sites were analyzed at the National Public Health Laboratory for identification. Antibiotic susceptibility testing (AST) was done using disk diffusion, minimum inhibitory concentration method following Clinical Laboratory Standard Institute (CLSI) guidelines. Results Among 214 pathogenic isolates from blood samples, E.coli was the most common (56.1%), followed by Salmonella Typhi/Salmonella Paratyphi A (18.7%), Staphylococcus aureus (13.5%), and Klebsiella pneumoniae (11.7%). Methicillin Resistance Staphylococcus aureus (MRSA) was detected in half of the isolates. E.coli was resistant to ampicillin (94.4%), trimethoprim-sulfamethoxazole (84.5%), and ceftriaxone (79.2%). Salmonella Typhi was resistant to ampicillin (73.3%) and trimethoprim-sulfamethoxazole (60.0%) and Salmonella Paratyphi A were resistant to fluoroquinolones (91.7%). For last resort antibiotics, E.coli was resistant to carbapenem groups (3.2% for imipenem, 4.9% for meropenem, and 5.0% for ertapenem). Klebsiella pneumoniae was not resistant to any groups. Conclusion E.coli was found at high rates in blood samples and was resistant to common antibiotics used in Cambodia. These pilot data show the importance of establishing a national AMR surveillance system in Cambodia to monitor AMR trends following GLASS guidelines. Disclosures All Authors: No reported disclosures

scholarly journals Prevalence of shigellosis and associated risk factors among undergraduate students of a private university in Ogun State, Nigeria

2021 ◽  
Vol 1 (1) ◽  
pp. 25-36
Author(s):  
Seyi Samson Enitan ◽  
Joan Odigie ◽  
Oluyemisi Ajike Adekunbi ◽  
Okondu Ogechukwu Emmanuel ◽  
Nwachi Idume Ogbonna ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Hand Hygiene ◽  
Undergraduate Students ◽  
Salmonella Typhi ◽  
Enteric Infection ◽  
Ogun State ◽  
Associated Risk Factors ◽  
Study Participants

This cross-sectional, institutional-based study aimed to determine the prevalence of shigellosis and associated risk factors among Babcock University students in Ogun State, Nigeria. One hundred and twenty (120) undergraduate students, 80 females and 40 males, were asked to provide stool specimen in a sterile leak-proof single-use universal bottle with a screw-capped lid. Standard microbiological techniques were used to analyse the stool samples. A questionnaire was designed to collect demographic and clinical information from participants. The data obtained were statistically analysed using the SPSS Statistics software (version 18.0). The study showed that there was no occurrence of shigellosis among the study participants (0%), but other enteric pathogens infection were present in all participants (100%). The three most common mono-infections found among the study participants included Staphylococcus aureus (40%), followed by Escherichia coli (16.7%), and Salmonella typhi and Bacillus spp. (3.3%, each). While the most common prevalence of dual-infections included Staphylococcus aureus and Escherichia coli (33.3%), followed by Staphylococcus aureus and Salmonella typhi (3.3%). The proportion of participants who were asymptomatic (66.7%) was significantly higher than the symptomatic ones (33.3%). Identified risk factors of non-Shigella enteric infection included consumption of beef, vegetables, fruits, poultry/poultry products, as well as street foods, poor hand hygiene before eating, anal cleaning, and poor hand hygiene after toileting. Although shigellosis did not exist among undergraduate students of Babcock University, this study highlighted the existence of other enteric infections among the study participants. Therefore, the necessary preventive measures should be ensured and sustained.

scholarly journals Antifungal and Antibacterial Activities of Apple Vinegar of Different Cultivars

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Driss Ousaaid ◽  
Hassan Laaroussi ◽  
Meryem Bakour ◽  
Hayat Ennaji ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Plate Method ◽  
Golden Delicious ◽  
Pathogenic Microbes

This study was designed to assess the antimicrobial potencies of apple vinegar against pathogenic microbes. The acidity and total phenolic content were carried out by titration with NaOH 0.1 N and the Folin–Ciocalteu method, respectively, while the spread plate method, agar well diffusion, and MIC assays were used to determine the antimicrobial activities of different vinegar samples. Acidity and phenolic content were dependent on the variety, where the highest values were observed in S2 with 4.02 ± 0.04% and 1.98 ± 0.05 mg GAE/mL for acidity and total phenolic content, respectively. The spread plate method revealed that samples S1 and S2 obtained from the Red delicious variety and Golden delicious variety, respectively, inhibit the growth of all tested strains, while S3 obtained from different varieties and S4 obtained from the Gala royal variety inhibit only two microbes (Escherichia coli and Vibrio cholerae). Sample S1 presented moderate antimicrobial effect against all examined strains with a diameter of inhibition ranging from 11 ± 0.7 to 19 ± 0.5 mm and with MIC values ranging between 1/2 and 1/100. The findings of the current study confirm the usefulness of apple vinegar as a natural sanitizer that inhibits the growth of pathogenic microbes.

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