scholarly journals UJI EFEKTIVITAS GEL KOMBINASI EKSTRAK ETANOL DAUN BANGUN-BANGUN (Coleus amboinicus L.) DAN DAUN KELAPA SAWIT (Elaeis guieensis Jacq) SEBAGAI OBAT LUKA BAKAR TAHUN 2020

2020 ◽  
Vol 3 (1) ◽  
pp. 62-68
Author(s):  
Evi Depiana Gultom
Keyword(s):  
Wound Healing ◽  
Elaeis Guineensis ◽  
Ethanolic Extract ◽  
Rabbit Skin ◽  
Iron Plate ◽  
Treatment Groups ◽  
Single Form ◽  
Gel Preparation ◽  
Coleus Amboinicus ◽  
All Treatment

Coleus amboinicus L leaves and Elaeis guieensis Jacq leaves contain various secondary metabolites such as saponins, tannins, alkaloids and flavonoids that function in wound healing. The purpose of this study was to determine the healing effect of burns using a gel preparation combination of ethanolic extract of Coleus amboinicus L. (EECAL)  And ethanolic extract of Elaeis guineensis Jacq. (EEEGJ) In healing burns. Coleus amboinicus L leaves and Elaeis guieensis Jacq leaves contain are separated from the petiole and then dried in a drying cupboard so that it becomes a simplicia. Simplisia is extracted by maceration method then evaporated with a rotary evaporator and evaporated again on a water bath to produce a thick extract. EECAL and EEEGJ are then formulated into gel preparations with varying concentrations. Each rabbit was shaved on its back then cleaned with 70% alcohol. Furthermore, rabbits were anesthetized using 0.5 ml Lidocain HCL as much as 0.5 ml subcutaneously. Then mark the part that will be injured with a diameter of 2 cm, by lifting the rabbit skin using tweezers and then made a wound using a hot iron plate that has been sterilized beforehand with 70% alcohol. Wound diameter measurements showed that all treatment groups from day 1 to day 20 experienced changes in wound diameter. EECAL 15% and EEEGJ 10% have a more effective effect in healing of burn than in their single form.           

scholarly journals EFEKTIVITAS SALEP KOMBINASI EKSTRAK DAUN BANGUN – BANGUN (Coleus amboinicus Lour.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) SEBAGAI OBAT LUKA SAYAT

2020 ◽  
Vol 3 (1) ◽  
pp. 48-56
Author(s):  
Rika Puspita Sari
Keyword(s):  
Wound Healing ◽  
Single Dose ◽  
Elaeis Guineensis ◽  
Ethanol Extract ◽  
Rabbit Skin ◽  
Treatment Groups ◽  
Healing Wound ◽  
Elaeis Guineensis Jacq ◽  
Coleus Amboinicus ◽  
All Treatment

Bangun-bangun leaves and palm leaves contain various secondary metabolites such as tannins, alkaloids and flavonoids which can healing wound. The aim of this study was to determine the effect of ointment combination of ethanol extract of leaves of bangun- bangun (Coleus amboinicus Lour.) And ethanol extract of palm leaves (Elaeis guineensis Jacq.) In healing wound. The bangun- bangun leaves and the palm leaves are separated from the petiole and then dried in a drying cupboard so that it becomes a simplisia. Simplisia is extracted by maceration method then evaporated with a rotary evaporator and evaporated again on a water bath to produce a thick extract. Thick extracts from the leaves of bangun- bangun and palm leaves are then formulated into ointment preparations with varying concentrations. Each rabbit was shaved on its back then cleaned with 70% alcohol. Furthermore, rabbits were anesthetized using 0.5 ml Lidocain HCL as much as 0.5 ml subcutaneously. Next mark the part that will be injured with a diameter of 2 cm, by lifting the rabbit skin using tweezers and then made a wound using surgical scissors that have been sterilized first with 70% alcohol. Wound diameter measurements showed that all treatment groups from day 1 to day 23 experienced changes in wound diameter. The combination ointment of ethanol extract of leaves wake-up (EEDB) 10% and ethanol extract of palm oil leaves (EEDKS) 10% have a more effective effect in wound healing than single dose.

scholarly journals THE EFFECTIVENESS OF COLEUS AMBOINICUS LEAF EXTRACTS AS AN ANALGETIC ACTIVITY ON MICE EXPOSURED TO ACETIC ACID

2018 ◽  
Vol 11 (9) ◽  
pp. 301 ◽  
Author(s):  
Yunita Sari Pane ◽  
Sufitni Sufitni ◽  
Fitriani Lumongga ◽  
Nurfida Alrasyid ◽  
Dini Permata Sari ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Water Extract ◽  
Ethanol Extract ◽  
Mean Value ◽  
Leukocyte Migration ◽  
Leaf Extracts ◽  
Mean Values ◽  
Treatment Groups ◽  
Coleus Amboinicus ◽  
All Treatment

Objective: This study aims to observe the analgesic activity of Bangun-bangun (Coleus amboinicus) leaves by induction of pain arising from chemical excitement after exposure to acetic acid.Methods: The effectiveness test of water extract and ethanol extract of Bangun-bangun leaves as an analgesic was measured by comparing the mean of quantitative and the writhing between control and treatment group. In addition, observations on the decrease in inflammatory processes occurred by calculating the reduction in the number of leukocyte migration to the inflammatory area.Result: The decrease of excitatory pain in all treatment Groups (I-IV) were significantly different. The comparison of mean values ± SEM decreased excitatory pain Groups I-II (255.00±22.22; 88.33±14.58), p=0.000. Groups I-III (255.00±22.22; 60.00±10.04), p=0.000 and Groups I-IV (255.00±22.22; 50.83±3.09), p=0.000. On histopathology examination, all treatment groups were significantly different (p=0.006). The comparison of mean value ± SEM decrease number of leukocyte Groups I-II (31.73±5.22; 14.70±3.71), p=0.025. Groups I-III (31.73±5.22; 15.67±3.22), p=0.036 and Groups I-IV (31.73±5.22; 11.77±2.77), p=0.008. However, the comparison of decrease of excitatory of pain and number of leukocyte migration between each group did not show significantly different (p>0.05).Conclusions: This study concluded that the Bangun-bangun leaves both in the form of water extract and ethanol extract proved efficacious as a pain reliever in the mice after induction of acetic acid.

scholarly journals Hypoxia Mesenchymal Stem Cells Accelerate Wound Closure Improvement by Controlling α-smooth Muscle actin Expression in the Full-thickness Animal Model

2021 ◽  
Vol 9 (A) ◽  
pp. 35-41
Author(s):  
Nur Fitriani Hamra ◽  
Agung Putra ◽  
Arya Tjipta ◽  
Nur Dina Amalina ◽  
Taufiqurrachman Nasihun
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Smooth Muscle ◽  
Wound Closure ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Full Thickness ◽  
Treatment Groups ◽  
All Treatment

BACKGROUND: The active myofibroblast producing extracellular matrix deposition regarding wound closure is characterized by alpha-smooth muscle actin (α-SMA) expression. However, the persistence of α-SMA expression due to prolonged inflammation may trigger scar formation. A new strategy to control α-SMA expression in line with wound closure improvement uses hypoxic mesenchymal stem cells (HMSCs) due to their ability to firmly control inflammation for early initiating cell proliferation, including the regulation of α-SMA expression associated with wound closure acceleration. AIM: This study aimed to explore the role of HMSCs in accelerating the optimum wound closure percentages through controlling the α-SMA expression. MATERIALS AND METHODS: Twenty-four full-thickness rats wound model were randomly divided into four groups: Sham (Sh), Control (C) by NaCl administration only, and two treatment groups by HMSCs at doses of 1.5×106 cells (T1) and HMSCs at doses of 3×106 cells (T2). HMSCs were incubated under hypoxic conditions. The α-SMA expression was analyzed under immunohistochemistry staining assay, and the wound closure percentage was analyzed by ImageJ software. RESULTS: This study showed a significant increase in wound closure percentage in all treatment groups that gradually initiated on days 6 and 9 (p < 0.05). In line with the increase of wound closure percentages on day 9, there was also a significant decrease in α-SMA expression in all treatment groups (p < 0.05), indicating the optimum wound healing has preceded. CONCLUSION: HMSCs have a robust ability to accelerated wound closure improvement to the optimum wound healing by controlling α-SMA expression depending on wound healing phases.

Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Emmanuel Tiyo Ayikobua ◽  
Josephine Kasolo ◽  
Keneth Iceland Kasozi ◽  
Ejike Daniel Eze ◽  
Abass Safiriyu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Side Effects ◽  
Motor Activity ◽  
Ethanolic Extract ◽  
Synergistic Action ◽  
Content Type ◽  
Treatment Groups ◽  
Ethanolic Extract Of Propolis ◽  
Different Doses

AbstractBackgroundThe Phosphatase and tensin-induced putative kinase 1 (PINK1B9) mutant for Drosophila melanogaster is a key tool that has been used in assessing the pathology of Parkinsonism and its possible remedy. This research was targeted toward determining the effects of ethanolic extract of propolis, with levodopa therapy in the management of Parkinsonism.MethodThe PINK1B9 flies were divided into groups and fed with the different treatment doses of ethanoic extract of propolis. The treatment groups were subjected to 21 days of administration of propolis and the levodopa at different doses after which percentage climbing index, antioxidant activity and lifespan studies were done.ResultsPropolis alone improved motor activity, antioxidant and lifespan in Drosophila melanogaster than in PINK1 flies. Propolis in combination with levodopa significantly (P<0.05) improved physiological parameters at higher than lower concentrations in Parkinsonism Drosophila melanogaster demonstrating its importance in managing side effects associated with levodopa.ConclusionPropolis is a novel candidate as an alternative and integrative medicinal option to use in the management of Parkinsonism in both animals and humans at higher concentrations.

scholarly journals Functional Monitoring after Trabeculectomy or XEN Microstent Implantation Using Spectral Domain Optical Coherence Tomography and Visual Field Indices—A Retrospective Comparative Cohort Study

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 273
Author(s):  
Marc Schargus ◽  
Catharina Busch ◽  
Matus Rehak ◽  
Jie Meng ◽  
Manuela Schmidt ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Cohort Study ◽  
Visual Field ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Treatment Groups ◽  
Rnfl Thickness ◽  
Comparative Cohort Study ◽  
The Mean ◽  
All Treatment

The aim of this study was to compare the efficacy of trabeculectomy (TE), single XEN microstent implantation (solo XEN) or combined XEN implantation and cataract surgery (combined XEN) in primary open-angle glaucoma cases, naïve to prior surgical treatment, using a monocentric retrospective comparative cohort study. Intraocular pressure (IOP) and the number of IOP-lowering drugs (Meds) were monitored during the first 24 months after surgery. Further disease progression was monitored using peripapillary retinal nerve fiber layer (RNFL) thickness examinations using spectral domain optical coherence tomography (OCT) as well as visual acuity (VA) and visual field (VF) tests. In the TE group (52 eyes), the mean IOP decreased from 24.9 ± 5.9 to 13.9 ± 4.2 mmHg (p < 0.001) and Meds decreased from 3.2 ± 1.2 to 0.5 ± 1.1 (p < 0.001). In the solo XEN (38 eyes) and the combined XEN groups, the mean IOP decreased from 24.1 ± 4.7 to 15.7 ± 3.0 mmHg (p < 0.001) and 25.4 ± 5.6 to 14.7 ± 3.2 mmHg (p < 0.001), while Meds decreased from 3.3 ± 0.8 to 0.8 ± 1.2 (p < 0.001) and 2.7 ± 1.2 to 0.4 ± 1.0 (p < 0.001), respectively. The VF and VA indices showed no sign of further deterioration, the RNFL thickness further decreased in all treatment groups after surgery. TE and XEN led to comparable reductions in IOP and Meds. Although the VA and VF indices remained unaltered, the RNFL thickness continuously decreased in all treatment groups during the 24-month follow-up.

Influence of Cisplatin on Wound Healing—An Experimental Model

1986 ◽  
Vol 95 (2) ◽  
pp. 210-212 ◽  
Author(s):  
Charles M. Stiernberg ◽  
R. Mark Williams ◽  
James A. Hokanson
Keyword(s):  
Wound Healing ◽  
Chemotherapeutic Agents ◽  
Optimal Time ◽  
Subcutaneous Injections ◽  
Treatment Groups ◽  
Cell Counts ◽  
Proliferative Stage ◽  
Significant Difference ◽  
Surgical Staples ◽  
Outbred Mice

Recent clinical studies have shown that adjuvant chemotherapy may improve response rates to treatment for advanced head and neck carcinomas. Given preoperatively, some chemotherapeutic agents adversely affect wound heallng. The specific purpose of this study was to evaluate the Influence of cisplatin on wound healing when it is given preoperatively. Forty-four Swiss outbred mice were divided into control and treatment groups. One week before surgery, the treatment group received cisplatin (2 mg/ kg body weight) by subcutaneous injections on 2 consecutive days. Each control animal was given an equal volume of normal saline. A 1.5 cm transverse incision was made in each animal, and wounds were closed with surgical staples. The mean woundbreaking strength was determined for a minimum of 5 treatment and 5 control mice on postoperative days 6, 10, 13, and 16. Serum creatinine, blood cell counts, and changes in weight were also monitored. Results showed wound strength on postoperative day 10 to be significantly reduced in mice treated with cisplatin (P < 0.05). There was no significant difference for wound strength on any other days and all other variables were simllar between both groups. In conclusion, cisplatin has an adverse effect on wound healing, the peak of which probably occurs during the proliferative stage of wound healing. Further studies are needed to determine the optimal time for surgery after preoperative chemotherapy. All new chemotherapeutic agents, particularly those being considered in a preoperative regimen, should be tested in this manner.

scholarly journals Biohydrogel Based on Dynamic Covalent Bonds for Wound Healing Applications

2021 ◽  
Vol 11 (15) ◽  
pp. 6945
Author(s):  
Chukwuma O. Agubata ◽  
Cynthia C. Mbaoji ◽  
Ifeanyi T. Nzekwe ◽  
César Saldías ◽  
David Díaz Díaz
Keyword(s):  
Wound Healing ◽  
Interfacial Tension ◽  
Blood Clotting ◽  
Phenylboronic Acid ◽  
Moisture Loss ◽  
Self Healing ◽  
Covalent Bonds ◽  
Treatment Groups ◽  
Acid Soluble Collagen ◽  
Thixotropic Behavior

In this work, a biohydrogel based on alginate and dynamic covalent B-O bonds, and derived composites, has been evaluated for wound healing applications. In particular, a phenylboronic acid–alginate (PBA-Alg) complex was synthesized by coupling 3-aminophenylboronic acid onto alginate, and used to prepare varied concentrations of hydrogels and silicate-based nanocomposites in PBS. The resulting hydrogels were characterized in terms of interfacial tension, moisture uptake and loss, interaction with fresh acid-soluble collagen, self-healing ability, effects on blood clotting and wound healing. The interfacial tension between the hydrogels and biorelevant fluids was low and moisture loss of 55%–60% was evident without uptake from the environment. The components of the hydrogels and their mixtures with collagen were found to be compatible. These hydrogels showed efficient self-healing and thixotropic behavior, and the animals in the treatment groups displayed blood clotting times between 9.1 min and 10.7 min. In contrast, the composites showed much longer or shorter clotting times depending on the silicate content. A significant improvement in wound healing was observed in 3% w/v PBA-Alg formulations. Overall, the PBA-Alg hydrogels exhibit self-healing dynamic covalent interactions and may be useful in dressings for incision wounds.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

scholarly journals Can Lipoic Acid Attenuate Cardiovascular Disturbances Induced by Ethanol and Disulfiram Administration Separately or Jointly in Rats?

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Anna Bilska-Wilkosz ◽  
Magdalena Kotańska ◽  
Magdalena Górny ◽  
Barbara Filipek ◽  
Małgorzata Iciek
Keyword(s):  
Blood Pressure ◽  
Rat Liver ◽  
Lipoic Acid ◽  
Aldh Activity ◽  
Dihydrolipoic Acid ◽  
Treatment Groups ◽  
Intracellular Compartments ◽  
Electrocardiogram Ecg ◽  
First Time ◽  
All Treatment

The exogenous lipoic acid (LA) is successfully used as a drug in the treatment of many diseases. It is assumed that after administration, LA is transported to the intracellular compartments and reduced to dihydrolipoic acid (DHLA) which is catalyzed by NAD(P)H-dependent enzymes. The purpose of this study was to investigate whether LA can attenuate cardiovascular disturbances induced by ethanol (EtOH) and disulfiram (DSF) administration separately or jointly in rats. For this purpose, we measured systolic and diastolic blood pressure, recorded electrocardiogram (ECG), and estimated mortality of rats. We also studied the activity of aldehyde dehydrogenase (ALDH) in the rat liver. It was shown for the first time that LA partially attenuated the cardiac arrhythmia (extrasystoles and atrioventricular blocks) induced by EtOH and reduced the EtOH-induced mortality of animals, which suggests that LA may have a potential for use in cardiac disturbance in conditions of acute EtOH intoxication. The administration of EtOH, LA, and DSF separately or jointly affected the ALDH activity in the rat liver since a significant decrease in the activity of the enzyme was observed in all treatment groups. The results indicating that LA is an inhibitor of ALDH activity are very surprising.

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