scholarly journals Increased Plasma GLP-1 Levels after Resistant Starch Type 3 From Cassava Starch (Manihot Esculanta Crantz) Diet on Diabetic Rat

2018 ◽  
Vol 4 (2) ◽  
pp. 116
Author(s):  
Elly Nurus Sakinah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Resistant Starch ◽  
Nutritional Therapy ◽  
Positive Control ◽  
Cassava Starch ◽  
Negative Control ◽  
Diabetic Rat ◽  
Sugar Levels ◽  
Type 3

Diabetes mellitus (DM) occur when human body is unable to produce enough insulin and/or unable to use insulin effectively, resulting in an increase of blood sugar levels (hyperglycemia). Indonesia  has  ranks  fourth  after  United  States,  China  and  India  in  the  prevalence  of  DM. Controling blood sugar and insulin levels can be done through the stimulation effect of Glucagon- like peptide-1 (GLP-1). The goal of this study was to determine the effects of resistant starch type 3 (RS3) from cassava starch in increasing plasma GLP-1 levels. This reasearch use male mouse (Ratttus novergicus) as a diabetic model by providing a high-fat diet (HFD) for one mounth than combine with intraoperitonel injection of low dose streptozotocin (STZ). The mouse then grouped into 4 groups randomly (1) normal or negative control, (2) cassava starch diet, (3) RS3 diet, (4) positive control. Blood sugar levels were measured before and after STZ injection to determine the diabetic conditions (blood sugar > 200 mg /dL). After 4 weeks of dietary administration blood sugar  and  plasma  GLP-1  levels  were  examined  using  ELISA.  Statistical  analysis  showed decreased in blood sugar levels and increased in plasma GLP-1 levels after one mounth of RS3 diet. This research shows that RS3 from cassava starch has a potential role as a nutritional therapy on diabetes mellitus condition. Keywords: Resistant starch type 3, cassava starch, GLP-1, Blood Sugar, Diabetes Mellitus

Improved cellular immunity and increased insulin in streptozotosin-induced mice using ethanol coriander (Coriandrum sativum) extract

2021 ◽  
pp. 3689-3694
Author(s):  
Dahliatul Qosimah ◽  
Sri Widyarti ◽  
MAG Beltran ◽  
Muhaimin Rifa’i
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Gastric Tube ◽  
Fat Metabolism ◽  
Relative Number ◽  
Positive Control ◽  
Negative Control ◽  
Coriandrum Sativum ◽  
Treatment Groups ◽  
Sugar Levels

An increase in blood sugar levels is an indication of diabetes mellitus (DM). DM is a metabolic disease characterized by disorders of carbohydrate and fat metabolism due to impaired activity, insulin secretion or both. Alternatively, the body's inability to secrete insulin. The purpose of this study was to determine the effect of coriander extract (Coriandrum sativum) on mice induced by streptozotocin (STZ) on the relative number of CD11b and insulin-IL6 cells. The study used Balb-C strain mice, aged 2.5-3 months, with five (5) treatment groups which were given STZ at a dose of 145mg/kg BW on the 8th day IP (Intraperitoneal) after adaptation for seven days, then add coriander extract with successive doses of 25 (T1), 50 (T2), and 100mg/kg BW (T3), negative control (healthy) (T4) and positive control (STZ) (T5) through the administration of intraperitoneal gastric tube. Blood Glucose is measured on the 13th day. Herbs are given on days 14-28. On the 29th day, the mice. An increase in blood sugar levels is an indication of diabetes mellitus (DM). DM is a metabolic disease characterized by disorders of carbohydrate and fat metabolism due to impaired activity, insulin secretion or both Alternatively, the body's inability to secrete insulin. The purpose of this study was to determine the effect of coriander extract (Coriandrum sativum) on mice induced by streptozotocin (STZ) on the relative number of CD11b and insulin-IL6 cells. The study used Balb-C strain mice, aged 2.5-3 months, with five (5) treatment groups which were given STZ at a dose of 145mg/kg BW on the 8th day IP (Intraperitoneal) after adaptation for seven days, then add coriander extract with successive doses of 25 (T1), 50 (T2), and 100mg/kg BW (T3), negative control (healthy) (T4) and positive control (STZ) (T5) through the administration of intraperitoneal gastric tube. Blood Glucose is measured on the 13th day. Herbs are given on days 14-28. On the 29th day, the mice were sacrificed Furthermore, the pancreas was taken for insulin examination, and the spleen was checked with the relative number of CD11b-IL6 and T CD4 cells using a flow cytometer. Data were quantitatively analyzed using the One Way ANOVA test (p≤0.05). The results showed that doses of coriander in T1 treatment causes an increase in insulin levels, decreased blood sugar levels, weight gain, proliferation and activation of CD4+ T cells, and decrease inflammation (CD11b cell-IL6) on the tissue. This research concludes that extracts of coriander can reduce Hyperglycemia in mice by modulation of cellular immunity and increased levels of insulin

scholarly journals UJI AKTIVITAS ANTIDIABETES KOMBINASI EKSTRAK ETANOL DAUN ECENG GONDOK (Eichhornia crassipes (Mart.)Solms) DAN DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 44-53
Author(s):  
Andri Priyoherianto ◽  
◽  
Panji Ratih Suci ◽  
Putri Rizki Cahya Fatimah ◽  
Atik Nur Wijayanti
Keyword(s):  
Blood Glucose ◽  
Blood Sugar ◽  
Water Hyacinth ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Glucose Levels ◽  
Normal Limits ◽  
Blood Glucose Levels ◽  
Sugar Levels

Diabetes mellitus is a chronic metabolic disorder that occurs due to destruction of pancreatic ? cells characterized by blood glucose levels that exceed normal limits. The purpose of this study was to determine the therapeutic effect of the combination of water hyacinth leaf extract and Sintrong leaf in reducing blood glucose levels in alloxan-induced experimental animals. Water hyacinth leaf powder and Sintrong leaves were extracted using the maceration method with 70% ethanol as a solvent. A total of 25 mice were divided into 5 groups, namely negative control CMC-Na 0.5%, positive control glibenclamide 0.00065 mg / g BW, water hyacinth leaf extract 17.5 mg / kg BW, Sintrong leaf extract 10.5 mg/ kg BW and a combination of water hyacinth leaf extract 17.5 mg / kg BW and sintrong leaf 10.5 mg / kg BW. The results showed that a single extract of water hyacinth and Sintrong leaves was able to reduce blood sugar levels for 7 days. The combination dose was effective in reducing blood sugar levels with a combination dose of 17.5 mg/ kg water hyacinth leaf extract in combination for 7 days.

scholarly journals Efektivitas Ekstrak Buah Merah (Pandanus conoideus De Vriese) Asal Kabupaten Poso Sebagai Alternatif Penurun Kadar Gula Darah

2017 ◽  
Vol 5 (4) ◽  
pp. 185
Author(s):  
Anang Wahid M. D. Diah ◽  
Ni Kadek Ana Diani ◽  
Minarni Rama Jura
Keyword(s):  
Free Radicals ◽  
Blood Sugar ◽  
Positive Control ◽  
Negative Control ◽  
Effective Concentration ◽  
Fruit Extract ◽  
Control Data ◽  
Significance Level ◽  
The Third ◽  
Sugar Levels

Bioactive compounds contained in red fruit (pandanus conoideus De Vriese) among others are flavonoids and tannins. The compounds are classified as very powerful antioxidants and can inhibit free radicals. This study aimed to determine the effective concentration of the red fruit extract from Poso as an alternative for lowering blood sugar levels. The separatin method used was boiling. The animals test were 15 male of mice (Mmus musculus) induced by ethylene diamine tetra acetic (EDTA). The mice were divided randomly into 5 groups with different treatments. The first, the second, and the third treatments were given red fruit extract each with a concentration of 10%, 20% and 30%. The fourth treatment was given glibenclamide suspension as a positive control, and the fifth treatments was given Na-CMC as a negative control. Data were analyzed using a statistical analysis of variance (Anova) test followed by Duncan test. The results showed that the preclinical test of red fruit extract reduced blood sugar levels of mice, and the most effective concentration was 20% as much as 68% (w/v) with significance level a = 0.05.

scholarly journals Resistant starch, amylose, and amylopectin content in breadfruit cookies as an alternative snack for individuals with diabetes mellitus

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 394-400
Author(s):  
Z.A. Fitriani ◽  
F.F. Dieny ◽  
A. Margawati ◽  
F.F. Jauharany
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Resistant Starch ◽  
Blood Sugar Level ◽  
Friedman Test ◽  
Lifestyle Management ◽  
Diet Management ◽  
Sugar Levels ◽  
The One ◽  
One Way Anova

Uncontrolled hyperglycaemia in individuals with diabetes mellitus can increase the risk factors for disease complications. Therefore, lifestyle management becomes a vital measure for those individuals, especially in diet management, to control their blood sugar level. Breadfruit starch-based cookies contain resistant starch, amylose, and amylopectin that can inhibit the increment of blood sugar levels. Hence, these cookies can be used as an alternative snack for those individuals. This study was aimed to analyse the content of resistant starch, amylose, and amylopectin, as well as the acceptability level of breadfruit starch-based cookies. We used a completely randomised experiment using four formulations with various breadfruit flour content: 25%, 50%, 75% and 100%. The breadfruit formulation containing breadfruit flour (BF) and wheat flour (WF). Formula 25% (25% BF 75% WF, 50% (50% BF and WF), 75% (75% BF 25% WF), and 100% BF. The one-way ANOVA was used to compare the content of resistant starch, amylose, and amylopectin among the cookies. Furthermore, the Friedman test was used to analyse the acceptance level of the cookies, which includes colour, odour, texture, and flavour. The cookies contain 19.38-20.51% of resistant starch, 13.55-16.60% of amylose, and 83.39- 86.44% of amylopectin. The highest resistant starch and amylose contents were found in cookies with 100% breadfruit flour content, while the highest amylopectin content was found in the 25% formulation. The acceptability level of the cookies included colour, odour, texture, and flavour. Cookies made with 100% breadfruit flour contained the highest resistant starch and amylose, but the lowest amylopectin. It also received the highest acceptability among the panellists.

scholarly journals Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan

2020 ◽  
Vol 1 (1) ◽  
pp. 24-31
Author(s):  
Rachmat Hidayat ◽  
Lusia Hayati
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Muscle Tissue ◽  
Leaf Extract ◽  
Negative Control ◽  
Diabetes Group ◽  
White Rats ◽  
Target Blood Glucose ◽  
Sugar Regulation ◽  
Sugar Levels

Diabetes mellitus is a chronic condition that disturbs the body's bloodsugar regulation. Bay leaves contain entirely various secondary metabolites,where this plant is rich in flavonoids, alkaloids, terpenes and glycosides. Thisstudy aims to assess the effect of bay leaf extract (Syzygium polyanthum) onblood sugar levels and the expression of GLUT4 protein in muscle tissue. A totalof 30 white rats (Rattus norvegicus) Wistar strain obtained from the EurekaResearch Laboratory (Palembang, Indonesia) weighing between 200 - 250 grams.Bay leaf simplicia was obtained from the Tawangmangu Herbal Research Center,Karanganyar, Indonesia. After 1 week of adaptation, the mice were randomlydivided into the following six groups, each containing 5 animals: Normal controlgroup, diabetes group (negative control), diabetes + metformin group (Met; 45mg / kg), Diabetes + BLE (75 mg/kg), diabetes + BLE group (150 mg / kg) anddiabetes + BLE group (300 mg/kg). Alloxan-induced white rats showed a verysignificant increase in blood sugar levels, where the use of the drug metforminwas able to reduce blood sugar levels significantly even though they had notreached the target blood glucose target of less than 200 mg / dL. The treatmentwith bay leaf extract was able to reduce blood sugar levels significantly. Theadministration of metformin drugs or bay leaf extract showed the ability toincrease the level of GLUT4 protein. In conclusion, bay leaf extract affectsreducing blood sugar levels in diabetes mellitus white rats by increasing glucoseintake to cells and tissues.

scholarly journals Innovation Of The Utilization Of Musa Acuminata Leather Waste To Be Cocupi (Coockies Banana Leather) As A Health Promotion In Prevention Of Diabetes Mellitus

2020 ◽  
Vol 14 (3) ◽  
pp. 187-200
Author(s):  
Mukhlisah Mukhlisah
Keyword(s):  
Diabetes Mellitus ◽  
Health Promotion ◽  
Human Life ◽  
Positive Control ◽  
Negative Control ◽  
Musa Acuminata ◽  
Banana Peel ◽  
Nutritional Content ◽  
Sugar Levels ◽  
Prevention Of Diabetes

Health is the most important thing in human life. Lifestyles and diets that are not suitable for the increase in the prevalence of regenerative diseases such as diabetes mellitus. Diabetes mellitus is a chronic disease characterized by a lack of the hormone insulin produced by the pancreas to regulate the amount of sugar levels in the blood. People do not realize that banana peels (Musa acuminata) can be used to prevent diabetes. Banana peel (Musa acuminata) has abundant nutritional content, including carbohydrates, protein, potassium. Based on other research articles, banana peels also contain a number of active compounds to prevent diabetes, namely pectin (10-21%), lignin (6-12%), cellulose (7.6% -9.6%), and hemicellulose (6). , 4-9.4%). Therefore, researchers have innovated to process banana peel waste (Musa acuminata) into COKUPI (Banana Skin Cookies) as a health promotion in preventing diabetes mellitus. The purpose of this study was to determine the potential of banana peel waste (Musa acuminata) as a prevention of diabetes mellitus. The benefit of this research is as a community guide in the utilization of banana peel waste (Musa acuminata). The material used in this study included banana peel cookies, alloxan, and mice. This study used an experimental method, namely by using the same 25 mice were given alloxan and measured blood sugar levels. After being measured, the white mice (Mus musculus) did fast for 18 hours. COKUPI was given with different treatments, namely negative control, positive control, doses of 100g, 200g, and 300g for 15 days. The results of this study were Kepok banana peels (Musa acuminata) which were processed into effective cookies as an alternative to prevent diabetes mellitus.

scholarly journals EFEKTIVITAS ANTIDIABETES EKSTRAK DAUN RAMBUSA (Passiflora foetida L.) PADA MENCIT (Mus musculus) DENGAN INDUKSI GLUKOSA

2015 ◽  
Vol 1 (2) ◽  
pp. 99-104
Author(s):  
Khildah Khaerati ◽  
Ihwan Ihwan ◽  
Musdalifah S Maya
Keyword(s):  
Blood Sugar ◽  
Leaf Extract ◽  
Drug Research ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antidiabetic Drug ◽  
Passiflora Foetida ◽  
Sugar Levels

Passiflora foetida leaves used by the society for generations as a natural antidiabetic drug research aims to test the effectiveness of the leaf extract of Passiflora foetida with variations of a dose of 250 mg / kg, 500 mg / kg, and 750 mg / kg in lowering blood sugar levels in mice. Passiflora foetida leaves were extracted by sokhletasi method with 70% ethanol, the extract was tested on 15 mice, the test animals were randomized into 5 groups consist of negative control group given 0.5% Na-CMC, positive control group was given glibenclamide 0, 65 mg / kg, and the treatment group were given the leaf extract of Passiflorafoetida with variations of each dose of 250 mg / kg, 500 mg / kg, 750 mg / kg. To increase blood sugar levels in mice induced glucose 50%. Measurement of blood sugar levels in mice were performed using an easy touch glucometer and blood is taken via the lateral tail vein. The results showed that the percentage of blood sugar levels drop to a negative control is 13.1%, for a dose of 250 mg / kg body weight of 29.52%, for a dose of 500 mg / kg was 38.79%, to dosis750 mg / kg was 49, 21%, and 48.1% for the positive control. The results showed that the leaf extract of Passiflorafoetida at a dose of 750 mg / kg is most effective as an antidiabetic

scholarly journals Type 3 Resistant Starch Effect of Cassava (Manihot esculenta Crantz) on Fasting Blood Glucose in Diabetes Mellitus Wistar Rat Models

2018 ◽  
Vol 4 (3) ◽  
pp. 133
Author(s):  
T Ariani Widiastini ◽  
Elly Nurus Sakinah ◽  
Yudha Nurdian ◽  
Jauhar Firdaus
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Resistant Starch ◽  
Manihot Esculenta ◽  
Fasting Blood Glucose ◽  
Cassava Starch ◽  
Control Group ◽  
Manihot Esculenta Crantz ◽  
Rat Models ◽  
Type 3

Type 2 Diabetes Mellitus (T2DM) is a condition in which both insulin secretion and insulin sentivity disturbed. One of therapeutic approach of T2DM is through diet modification using resistant starch (RS) that has proven controlling both postprandial and fasting blood glucose. Cassava starch has 28,57% amylose and 51,24% amylopectin that can be increased through autoclaving-cooling process. Therefore, it can resist the digestion proess by amylose enzyme. The aim was to describe the effect of type 3 resistant starch cassava (Manihot esculenta Crantz) on fasting blood glucose (FBG) in Wistar diabetic rat models. This study was quasy experimental post-test only control group design using 16 Wistar rats divided into 4 groups, KN (normal), K- (DM), P1 (DM + cassava starch diet), and P2 (DM + type 3 RS cassava diet). High fat diet (HFD) and low dose streptozotocin (STZ) used as diabetic agents. Rats fed cassava starch or type 3 RS cassava diet for 28 days. GOD-PAP method used to measure the FBG level. The data analyzed using One Way ANOVA test with p>0,05 as significant value. The result shows no significant differences within experimental groups. Keywords: cassava, autoclaving-cooling, resistant starch, FBG

scholarly journals Pengaruh Ekstrak Tanaman Putri Malu (Mimosa pudica Linn) Terhadap Penurunan Kadar Glukosa Darah Pada Mencit (Mus musculus)

2017 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Kasmudin Mustapa ◽  
Amalia Rizky ◽  
Minarni Rama Jura
Keyword(s):  
Blood Sugar ◽  
Plant Extract ◽  
Positive Control ◽  
Negative Control ◽  
Effective Concentration ◽  
Mimosa Pudica ◽  
Group Vi ◽  
Test Analysis ◽  
Group I ◽  
Sugar Levels

The research aim is to determine the effect of mimosa plant (mimosa pudica Linn) to reduce blood sugar levels in mice and determining the effective concentration of mimosa plant extracts as an alternative to decrease blood sugar levels. The extract is produced by the infusion method. The animal test used is male mice with amount 18 mice that induced by EDTA (ethylene diamine tetraacetic acid). Mice are divided into 6 groups randomly with different treatment. The group I, II, III and IV were given the mimosa plant extract with a concentration of 10, 20, 30, and 40% (w/v) respectively while group V is given glibenclamide suspension as positive control (+) and group VI is given Na-CMC 1% as negative control (-). Data were analyzed using the statistical test analysis of variance (ANOVA) and then followed by Duncan test. The results showed that the mimosa plant extract contains flavonoids, alkaloids, and tannins. In preclinical testing of mimosa plant extract proven to reduce blood sugar levels in mice and the most effective concentration is a concentration of 20% (w/v) with significantly α = 0.05.

scholarly journals AKTIVITAS ANTIHIPERGLIKEMIK PATI TAPIOKA TERMODIFIKASI EKSTRAK DAUN JAMBU BIJI MERAH PADA TIKUS DIABETES [ANTIHYPERGLICEMIC ACTIVITY OF TAPIOCA STARCH MODIFIED WITH RED GUAVA LEAF EXTRACT ON DIABETIC RAT]

2020 ◽  
Vol 43 (1) ◽  
pp. 1-10
Author(s):  
Elisa Diana Julianti ◽  
Nunung Nurjanah ◽  
Deddy Muchtadi ◽  
Nurheni Sri Palupi ◽  
Dewi Ratih Agungpriyono
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Leaf Extract ◽  
Diabetic Rat ◽  
Sprague Dawley ◽  
Tapioca Starch ◽  
Antihyperglycemic Activity ◽  
Sugar Levels ◽  
Guava Leaves ◽  
Guava Leaf

Guava leaves extract were known to be rich in polyphenols and had an antihyperglycemic activity. Carbohydrate source food modified with polyphenols had lower digestibility and glycemic index than native. For that reason, tapioca (Manihot utilissima) modified with guava leaves extract was expected for reducing the risk of diabetes mellitus. The antihyperglycemic activity of tapioca starch modified with red guava leaf extract and its native starch were evaluated in streptozotocin-induced diabetic Sprague Dawley rats aged 2 months (weight 175-250 g). Rats were fed with tapioca starch modified with 4 percent guava leaf extract and native tapioca for 35 days. The rats, the blood glucose level, and pancreatic Langerhans islets were assayed. The result showed that tapioca starch modified with 4 percent guava leaves extract had antihyperglycemic activity compared to its native starch. Tapioca starch modified with 4 percent guava leaves extract significantly increased beta-cell pancreas density and even though it did not significantly lower blood sugar levels in diabetic rats, but at the end of treatment, blood sugar levels were lower than the diabetic rat fed with native tapioca.ABSTRAK Ekstrak daun jambu biji diketahui kaya akan polifenol dan memiliki aktivitas antihiperglikemik. Modifikasi pangan sumber karbohidrat dengan polifenol memiliki daya cerna dan indeks glikemik lebih rendah dibanding aslinya. Oleh karena itu pati tapioka (Manihot utilissima) termodifikasi ekstrak daun jambu biji merah diharapkan dapat mengurangi risiko diabetes mellitus. Aktivitas antihiperglikemik dari pati tapioka termodifikasi dengan ekstrak daun jambu biji merah dan pati aslinya dievaluasi menggunakan tikus Sprague Dawley berusia 2 bulan (175-250 g) yang diinduksi dengan streptozotocin. Tikus diabetes diberi pakan tapioka terdimodifikasi 4 persen ekstrak daun jambu dan tapioka asli sebagai pembanding selama 35 hari. Parameter yang dinilai adalah glukosa darah dan gambaran histopatologis pankreas tikus. Hasil menunjukkan bahwa tapioka termodifikasi 4 persen ekstrak daun jambu memiliki aktivitas antihiperglikemik dibandingkan pati aslinya. Tapioka termodifikasi ekstrak daun jambu biji merah secara signifikan meningkatkan kepadatan sel beta pankreas dan meskipun tidak signifikan menurunkan kadar gula darah pada tikus diabetes, namun pada akhir perlakuan, kadar gula darah lebih rendah dibandingkan kelompok tikus diabetes yang diberi pakan tapioka asli.

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