scholarly journals Toothpaste Activity Test of Laban Leaf Methanol Extract (Vitex pinnata) Against the Growth of Streptococcus mutans Bacteria

2021 ◽  
Vol 9 (F) ◽  
pp. 95-100
Author(s):  
Cut Nuraskin ◽  
Reca Reca ◽  
Teuku Salfiyadi ◽  
Abdurrahman Abdurrahman ◽  
Teuku Iskandar Faisal ◽  
...  
Keyword(s):  
Particle Size ◽  
Streptococcus Mutans ◽  
Methanol Extract ◽  
Ph Value ◽  
Antibacterial Properties ◽  
Control Group ◽  
Foam Formation ◽  
Test Results ◽  
Anova Test ◽  
Activity Test

BACKGROUND: One of the natural ingredients that can be used as a substitute for preventing dental caries is the methanol extract of Laban leaves. Laban contains various compounds that are known to have antibacterial properties. The traditional ability of Laban Leaf Methanol Extract has been proven to treat diseases caused by pathogenic microorganisms. AIM: The aim of the study was to analyzing the activity test of Laban leaf methanol extract toothpaste (Vitex pinnata) against the growth of Streptococcus mutans bacteria. METHODS: This type of laboratory experimental research (true experimental design) with post-test only control group design. This research was conducted at the Poltekkes Pharmacy Laboratory of the Ministry of Health in Aceh in July-August. The research sample consisted of Laban leaves collected randomly in the Ie Seu Um Geothermal area of Selawah Agam Aceh Besar. Data analysis was carried out descriptively. Antibacterial activity data were statistically processed with one-way ANOVA at a confidence level of α 0.05. RESULTS: The organoleptic test results observed for 3 weeks showed that there were differences in color and aroma; the higher the concentration of the resulting color, the darker it was, and resulting in a soft, sweet taste in the toothpaste of laban leaf methanol extract. The pH acidity test results showed an effect of storage time (the week I, week II, and week III) on toothpaste’s pH value. The viscosity test results showed that the toothpaste formula combined with the methanol extract of Laban leaves met the SNI 12-524-1995 standards regarding toothpaste. Toothpaste formula F3. 4.5% has the greatest viscosity, ranging from 241.0 (PDAs), and followed by F2. 240.4%, and F1. 237.6%. The higher the active ingredient concentration, the greater the viscosity value. The foam formation test results show that the foam’s stability is greatly influenced by particle size. The more the particle size, the lower the foam stability. ANOVA test results based on the concentration of significance value (0.000) <0.05, there is a difference in the number of bacterial colonies based on the concentration variable. ANOVA test results based on the repetition of significant values (0.423)> 0.05, which means that all repetitions have the same effect on the number of colonies, so there is no difference in the number of colonies based on the repetition variable. CONCLUSION: Minimum inhibitory concentration of toothpaste laban leaf methanol extract against S. mutans growth was aimed at a concentration of 4.5%, with an average number of bacterial colonies 108.5 × 10−7 CFU/mL).

scholarly journals The role of Kuniran (U. moluccensis) and Gurami (O. goramy) fish thorns and scales in increasing salivary leukocyte and monocyte cells viability against Streptococcus mutans

2019 ◽  
Vol 52 (1) ◽  
pp. 45
Author(s):  
I Dewa Ayu Ratna Dewanti ◽  
I Dewa Ayu Susilawati ◽  
P. Purwanto ◽  
Pujiana Endah Lestari ◽  
Roedy Budirahardjo ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Microtiter Plate ◽  
Control Group ◽  
Fish Scales ◽  
Test Results ◽  
Omega 3 ◽  
Viability Analysis ◽  
Omega 6 ◽  
3 Omega

Background: Kuniran thorns and Gurami fish scales are rich in protein and minerals such as dentin believed to increase cell viability against Streptococcus mutans (S. mutans) that causes dental caries. These, in turn, can cause systemic diseases if left untreated. Purpose: This study aims to analyze the influence of Kuniran thorns and Gurami fishes scales on the viability of monocytes and salivary leukocytes against S. mutans. Methods: Monocytes and leukocytes salivary cells were placed on a microtiter plate and treated according to the nature of each group. This study comprised the following groups: control group: untreated; S. mutans group: induced by S. Mutans; Gurami thorn group: thorns + S. Mutans; Gurami scales group: scales + S. Mutans; Kuniran thorn group: thorns + S. Mutans; Kuniran scales group: scales + S. mutans. Viability analysis involved staining with Tripan Blue. Furthermore, the number of viable cells (white) was calculated under an inverted microscope at 200 times magnification from five fields of view. Data was analyzed by means of an ANOVA test followed by LSD test. Results: The ANOVA and LSD tests confirmed significant differences (0.01<P). Kuniran thorns and Gurami fish scales increased the viability of monocytes and salivary leukocytes, but not significantly. The content of flavonoids, amino acids, omega 3, omega 6 and antioxidants increased leukocyte metabolism, thereby increasing cell resistance to S. mutans infection. Conclusion: Kuniran thorns (U. moluccensis) and Gurami (O. goramy) fish scales increase the viability of salivary leukocyte and monocyte cells against Streptococcus mutans.

scholarly journals Effects of the fixed orthodontic therapy on biochemical and microbiological parameters of saliva

2020 ◽  
Vol 148 (5-6) ◽  
pp. 270-274
Author(s):  
Tanja Ivanovic ◽  
Ljiljana Stojanovic ◽  
Dragan Ivanovic ◽  
Predrag Nikolic ◽  
Zeljko Milosavljevic ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Ph Value ◽  
Periodontal Diseases ◽  
Buffering Capacity ◽  
Control Group ◽  
Orthodontic Appliances ◽  
Orthodontic Therapy ◽  
Fixed Orthodontic Appliances ◽  
Number Of Bacteria ◽  
Lactobacillus Spp

Introduction/Objective. Malocclusions are one of the most frequent disorders in dentistry, and pose a risk for the onset of caries and periodontal diseases. Fixed orthodontic treatment solves the problem of malocclusions; however, it requires simultaneous cooperation of the patients, parents, and dentists involved. The objective of this study is to examine the effects of fixed orthodontic therapy on the Streptococcus mutans and Lactobacillus spp. bacteria in saliva, the pH value, and buffering capacity of saliva. Methods. The research was carried out at the Faculty of Medicine in Foca, Department of Dentistry. The study included 100 respondents, aged 13 to 17 years. The respondents were divided into two groups: the study group (respondents wearing fixed braces) and the control group (respondents not subjected to fixed braces therapy). Saliva samples were taken from the respondents four, 12, and 18 weeks after the start of the orthodontic therapy. The study used the bacteria caries risk test (CRT) and CRT buffer (Ivoclar Vivadent). Results. The study showed an increased number of bacteria in saliva of the respondents during all three follow-up periods (?2 test, p = 0.001). The largest numbers of the Streptococcus mutans and Lactobacillus spp. bacteria were found in week 12 of the therapy. Saliva pH value and buffering capacity of saliva increased statistically significantly in week 12 of the therapy (?2 test, p = 0.001). Conclusion. Oral conditions in patients changed during the fixed orthodontic therapy: the number of bacteria increased, the pH value and buffering capacity of saliva changed. It was necessary to use preventive measures in order to avoid complications during the fixed orthodontic appliances therapy.

scholarly journals EFFECT OF BREADFRUIT LEAF EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS BIOFILM IN VITRO

2019 ◽  
pp. 96-99
Author(s):  
SEPTI WARDA ZULFIKAR ◽  
SRI UTAMI ◽  
RATNA FARIDA
Keyword(s):  
Oral Cavity ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
The Other ◽  
Control Group ◽  
Maturation Phase ◽  
Accumulation Phase

Objective: Breadfruit leaf has potent antibacterial properties that could be used to reduce biofilms in the oral cavity. The purpose of this study was toanalyze the antibacterial effect of the breadfruit leaf extract on the growth of Streptococcus mutans in vitro.Methods: S. mutans ATCC 25175 was cultured in a 96-well plate and incubated at 37°C for 20 h (accumulation phase) and 24 h (maturation phase).The breadfruit leaf extract was added at the following concentrations: 5%, 10%, 15%, 20%, 40%, 80%, and 100%. The viability of S. mutans was testedwith the MTT assay at a wavelength of 490 nm. The results were analyzed by one-way analysis of variance.Results: In the accumulation phase, a significant decrease was found in S. mutans viability at different concentrations of the breadfruit leaf extract, butin the maturation phase, a significant decrease was found in the S. mutans viability at the 5% concentration. The other groups decreased significantlycompared with the control group (*p<0.05). The viability of S. mutans after adding the breadfruit leaf extract at all concentrations was lower in theaccumulation phase than that in the maturation phase.Conclusion: In the accumulation phase, breadfruit leaf extract at concentrations of 5%, 10%, 20%, 40%, 80%, and 100% can reduce S. mutans biofilmviability.

scholarly journals Antimicrobial Activity Jangjingki (Oxalis corniculata L.) Against The Growth Of Staphylococcus Aureus, Escherichia Coli, and Candida Albicans

2021 ◽  
Vol 4 (1) ◽  
pp. 1-11
Author(s):  
Misrahanum Misrahanum ◽  
Syarifah Dhea Almunawwarah ◽  
Hira Helwati ◽  
Hilda Maysarah ◽  
Sadli Sadli
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Water Soluble ◽  
Test Results ◽  
Soluble Extract ◽  
E Coli ◽  
Activity Test ◽  
Water Soluble Extract

Jangjingki (Oxalis corniculata L.) is a plant from Oxalidaceae that has potential as a natural antimicrobial agent. The purpose of this research is to see the antimicrobial activity of methanol extract of jangjingki against the growth of Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. The thick extract of jangjingki was obtained by the maceration method with methanol solvent. At the same time, the antimicrobial activity test on S. aureus and E. Coli bacteria was carried out using the hole diffusion method. The simplicia characterization showed 7.17% water, 9.68% of total ash, 11.67% water-soluble extract, and 9.17% of the ethanol-soluble section. At the same time, the methanol extract of jangjingki characterization showed 22.5% of water, 10.16% of total ash, 55.83% water-soluble extract, and 62.91% of the ethanol-soluble section. Phytochemical test results showed that the methanol extract of jangjingki contains alkaloids, saponins, flavonoids, tannins, and steroids. The results of the antimicrobial activity test with variations in the concentration of jangjingki methanol extract 15, 20, 30, and 40% on S. aureus and E. Coli bacteria showed growth inhibition activity of these two bacteria, the largest diameter of the inhibition zone was formed when the extract was given a concentration of 40% with a diameter of the area. Resistance of 8,07 mm and 11 mm, respectively. Meanwhile, the test results of inhibition of growth of the fungus C. Albicans by presenting variations in the concentration of jangjingki methanol extract of 5, 10, 15, and 20% could not inhibit the growth fungus C. Albicans.

scholarly journals Effect of Pit and Fissure Sealants on the Prevention of Enamel Demineralization After Exposure to Streptococcus mutans Biofilm: An Vitro Study

2020 ◽  
pp. 12-19
Author(s):  
Patrícia da Silva Lopes Pereira DA SILVA
Keyword(s):  
Streptococcus Mutans ◽  
Metabolic Activity ◽  
Negative Control ◽  
Control Area ◽  
Test Results ◽  
Fissure Sealants ◽  
Enamel Demineralization ◽  
Activity Test ◽  
Natural Flow ◽  
Knoop Microhardness

Objective: The aim of this study was to evaluate the effect of sealants on the prevention of enamel demineralization and on biofilm metabolic activity. Materials and Methods: Cavity preparations were performed on 45 blocks of bovine teeth (4x4x4 mm) randomly assigned to three groups (n=15): RI-Riva Light Cure®/SDI; EM-Embrace™ WetBond™ Pulpdent Corp.®; and CO-Natural Flow/DFL resin (negative control). The sealed blocks were subjected to thermocycling (500 cycles/30 s). Half of the blocks were covered with acidresistant varnish to create a control area (RI, EM, and CO) and an experimental area (RI-EX, EM-EX, and CO-EX). They were subsequently exposed to Streptococcus mutans biofilm for assessment of demineralization by the Knoop microhardness test (50 g/15 s), at 25, 50, 100, 150, and 200 μm from the interface. In another test, the cylinders of the sealants (4 mmx3 mm) were subjected to the biofilm metabolic activity test. Results: The RI-EX group showed higher microhardness than CO-EX (p<0.05) and less demineralization when compared to RI at all distances. Microbial activity was lower in EM compared to CO, but not statistically significant in relation to RI. Conclusion: Both sealants can inhibit enamel demineralization in the presence of S. mutans biofilm.

scholarly journals Antibacterial Mouthwash of Kesum Leaf (Polygonum Minus Huds) Essential Oil on Streptococcus Mutans and Sanguinis

2020 ◽  
Vol 18 (2) ◽  
pp. 128-136
Author(s):  
Sri Rezki ◽  
Halimah Halimah
Keyword(s):  
Essential Oil ◽  
Streptococcus Mutans ◽  
Anaerobic Bacteria ◽  
Antibacterial Properties ◽  
Negative Control ◽  
Chemistry Laboratory ◽  
Control Group ◽  
Control Group Design ◽  
Streptococcus Sanguinis ◽  
Polygonum Minus

Polygonum Minus Huds methanol extract from several previous studies has antibacterial properties in bacteria in the mouth such as Streptococcus Mutans, Staphylococcus Aureus, Salmonella Typhi, Escherichia Coli, etc. The cause of gingivitis is Streptococcus Sanguinis and anaerobic bacteria in the subgingival, which is porphyromonas gingivalis. Polygonum Minus Huds has the potential to be an antibacterial mouthwash for bacteria that causes caries and gingivitis. This research aims to make a mouthwash based on the essential oil of Polygonum Minus Huds and determine its antibacterial properties in mouthwashes of 0.025% and 0.05% concentrations to find antibacterial activity on Streptococcus Mutans and Sanguinis. The research was conducted in June 2019 at the Chemistry Laboratory of Faculty of Mathematics and Natural Sciences, the University of Tanjungpura in Pontianak Lab and Health Polytechnique Pontianak Integrated Lab. Design Quasi-experimental research used post-test only control group design, mouthwash formulation with essential oil concentrations of 0.025% and 0.05%, positive control using non-alcoholic mouthwash, and negative control (Aquades). Statistical analysis was performed univariately. The results showed that the best mouthwash was 2.5 ml of Tween 80 and 2.5 ml of glycerin. The results showed the essential oil gargle of Kesum leaves (Polygonum Minus Huds) at concentrations of 0.05%, and 0.02%, which is the bacteria of Streptococcus Mutans and Streptococcus Sanguinis were not able to inhibit the growth of the two bacteria. Increasing the amount of concentration or making mouthwash in other forms is expected to increase its antibacterial effect.

Antibacterial Effect of Experimental Orthodontic Adhesives Containing Gold Nanoparticles against Streptococcus mutans and Streptococcus sobrinus

2021 ◽  
Vol 904 ◽  
pp. 301-308
Author(s):  
Pinyada Akarajarasrod ◽  
Surachai Dechkunakorn ◽  
Pornpen Tantivitayakul ◽  
Primana Punnakitikashem ◽  
Wassana Wichai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Bacterial Growth ◽  
Bacterial Colonization ◽  
Antibacterial Properties ◽  
Control Group ◽  
White Spot Lesion ◽  
Streptococcus Sobrinus ◽  
Orthodontic Adhesives

Enamel decalcification usually can be found around orthodontic bracket after debonding. Orthodontic adhesives resistant to bacterial colonization were used to prevent white spot lesion and dental caries in orthodontic treatment with fixed appliance. The objectives of this study were to evaluate the antibacterial properties of expeimental orthodontic adhesive containing gold nanoparticles (AuNPs). 108 orthodontic adhesive discs containing 1.0 wt%, 0.5 wt%, 0.25 wt% AuNPs and without AuNPs were prepared from in-house orthodontic adhesive (27 discs per group). The antibcterial properties of adhesive discs were evaluated by direct contact test. Streptococcus mutans and Streptococcus sobrinus suspensions were placed on the discs and incubated at 37 °C for 1 hour. The adhesive discs were transferred to BHI broth and were incubated at 37°C for 16, 20 and 24 h. Bacterial growth was evaluated by optical density (OD) measurement at 590nm. The results showed that experimental orthodontic adhesives with 1.0 wt% and 0.5 wt% AuNPs exhibited higher antibacterial activity compared to the control group (p < 0.05). At 24 h of incubation, the median OD of 1.0 wt%, 0.5 wt%, 0.25 wt% and control in S. mutans were 0.109, 0.006, 0.007 and 0.372, rescpectively. In S. sobrinus, the median OD were 0.173, 0.012 , 0.007 and 0.328. The incorporation of gold nanoparticles into orthodontic adhesives exhibited antibacterial activity against cariogenic microorganism. At a concentration of 0.5 wt% and 1.0 wt% AuNPs, experimental orthodontic adhesive could reduce bacterial growth of both S. mutans and S. sobrinus.

scholarly journals Pump It Up and Zig Zag Run Training Improve Children Agility Age 7-8 Years Old

2020 ◽  
Vol 9 (1) ◽  
pp. 54-59
Author(s):  
Kurniani Fatma Hardini ◽  
Gadis Meinar Sari ◽  
Choesnan Effendi
Keyword(s):  
Experimental Research ◽  
Test Group ◽  
Control Group ◽  
Test Results ◽  
Anova Test ◽  
Group Design ◽  
Test Instrument ◽  
Post Test ◽  
Shuttle Run

This study used experimental research, with a Randomized Pre and Post Test Group Design. This study consisted of 3 groups: the Pump It Up group, the Zig Zag Run game group, and the Shuttle Run game control group with a sample of each group of 10. The Agility Illionis Test was chosen as the agility test instrument. The exercise was done three days a week for four weeks. Anova test results showed that there was a significant effect of Pump It Up and Zig Zag Run exercises on agility with indigo p <0.05, and LSD test showed there were differences in Pump It Up with Ziz Zag Run, there were differences in Pump It Up and Zig Zag Run with indigo p <0.05. The conclusion of this research was that Pump It Up and Zig Zag Run exercises increase agility

scholarly journals Antibacterial Activity Tests of N-hexane, Ethyl Acetate, and Methanol Leaves (Vitex) Extract (pinnata) against Streptococcus mutans

2020 ◽  
Vol 8 (A) ◽  
pp. 181-184
Author(s):  
Cut A. Nuraskin ◽  
Marlina Marlina ◽  
R. Idroes ◽  
C. Soraya ◽  
Djufri Djufri
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Standardized Test ◽  
Raw Materials ◽  
Animal Health ◽  
Control Group ◽  
Growth Media

BACKGROUND: Vitex pinnata is known as Laban, which is a medicinal plant used traditionally for generations to generations. Laban leaf extract with various concentrations has antibacterial activity. Laban leaf extract is known to inhibit the formation of Streptococcus mutant in human teeth. AIM: To exam, the minimum inhibitory concentration (MIC) and minimum kill concentration (KBM) extract Laban leaves (V. pinnata) as an antibacterial against Streptococcus mutants. METHODS: This research was purely experimental research with design randomized pretest-posttest and control group. The study was conducted at the Laboratory Microbiology Faculty of Animal Health, Syiah Kuala University, Banda Aceh, from March to June 2019. The sample of this study was the Laban leaf from the Aceh Besar geothermal area. This research included preparation raw materials, chemical characterization of raw materials, determination of minimum and maximum components mixture, determining the optimum formula based on the best inhibitory potential, inhibitory testing antibacterial, standardized test, and test (one-way ANOVA). RESULTS: MIC of n-hexane extract showed the results of calculations; the percentage of bacterial inhibition was at a MIC of 1.56% on average colony −1.45 CFU/ml. In ethyl acetate extract, the MIC was 0.20% on the average colony −0.17 CFU/ml. The methanol extract can inhibit bacteria at the smallest concentration of 0.05% average colony −1.48 CFU/ml. Methanol extract inhibits bacteria more quickly. Concentration results minimum kill was 1.56%, 0.78%, 0.39%, 0.20%, 0.10%, and 0.05%. The smallest concentration of extract (n-hexane, ethyl acetate, and methanol) can kill Streptococcus mutans bacteria that are marked in the absence of bacterial colonies on microbiological growth media. CONCLUSION: Extracts of n-hexane, ethyl acetate, and methanol from Laban leaves have inhibitory activity on the growth of S. mutans bacteria. The smallest concentration of extract (n-hexane, ethyl acetate, and methanol) is able to kill S. mutans bacteria.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L)

2019 ◽  
Vol 7 (1) ◽  
pp. 25-31
Author(s):  
Fensia Analda Souhoka ◽  
Nikmans Hattu ◽  
Marsye Huliselan
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Flavonoid Compound ◽  
Bixa Orellana ◽  
Test Results ◽  
Red Pigment ◽  
Absorbance Measurement ◽  
Activity Test ◽  
Ic50 Value ◽  
Deterrent Activity

Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.

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