scholarly journals Antioxidant Activity of Purslane (Portulaca oleracea L.) Leaf Extract on the Levels of Ovarian Oxidative Stress and Reproductive Hormone in Rattus norvegicus Exposed to Cigarette Smoke

2021 ◽  
Vol 9 (B) ◽  
pp. 1535-1540
Author(s):  
Astika Gita Ningrum ◽  
Endyka Erye Frety ◽  
Ivon Diah ◽  
Zidni Hasbuna Shabran ◽  
Rida Eka Setiani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Portulaca Oleracea ◽  
Wild Plant ◽  
Assay Method ◽  
Reproductive Hormone ◽  
White Rats

BACKGROUND: Purslane (Portulaca oleracea L.) is a wild plant that grows in Indonesia and is considered a weed, but it has the highest antioxidant activity in the leaves and stems. Cigarette smoke is one of the exogenous free radicals that can enter blood circulation, interfering with all cells and tissues in the body, including reproductive organs. AIM: The research aims to examine the antioxidant effect of purslane leaf extract on oxidative stress levels (ovarian malondialdehyde [MDA] levels) and reproductive hormone levels (follicle-stimulating hormone [FSH] and 17ß-estradiol) in female white rats exposed to cigarette smoke. METHODS: This study is an experimental study with randomized post only control group design, using 12-week-old female Wistar strain Rattus norvegicus weighing about 200–250 g divided into four treatment groups (n = 6). Negative control without exposure to cigarette smoke and purslane leaf extract (K), cigarette group with cigarette smoke 1 stick/day administration without purslane leaf extract (P1), purslane group with purslane leaf extract 200 mg/Kg BW without exposure to cigarette smoke (P2), and group the group with cigarette smoke 1 stick/day accompanied by purslane leaf extract 200 mg/Kg BW administration (P3). Ovarian MDA measurement measures MDA levels as an indicator of oxidative stress in the ovaries determined by the spectrophotometry method. The enzyme-linked immunosorbent assay method measured the measurement of FSH and 17ß-estradiol levels in blood taken intracardially through the right ventricle of the heart. RESULTS: The results showed that purslane leaf extract at a 200 mg/Kg BW dose reduced ovarian MDA levels of female white rats exposed to cigarette smoke significantly with p < 0.05. CONCLUSION: However, there has been no significant change in FSH and 17ß-estradiol.

Antioxidant activity of purslane (portulaca oleracea l.) Leaf extract on ovarian oxidative stress levels and reproductive hormone levels of female white rats (rattus norvegicus) exposed to cigarette smoke

2021 ◽  
Vol 15 (8) ◽  
pp. 2279-2283
Author(s):  
Astika Gita Ningrum ◽  
Endyka Erye Frety ◽  
Ivon Diah Wittiarika ◽  
Zidni Hasbuna Shabaran ◽  
Rida Eka Setiani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Cigarette Smoke ◽  
Leaf Extract ◽  
Follicle Stimulating Hormone ◽  
Portulaca Oleracea ◽  
Control Group ◽  
Reproductive Hormone ◽  
White Rats ◽  
Estradiol Levels

Purslane (Portulaca oleracea L.) is a wild plant that grows in Indonesia and is considered a weed, but the highest antioxidant activity was found in the leaves and stems. Cigarette smoke is one of the exogenous free radicals that can enter the blood circulation, so that it can interfere with all cells and tissues in the body, including the reproductive organs. The research that will be conducted is about the antioxidant effect of purslane leaf extract on oxidative stress levels, namely ovarian malondialdehyde levels and reproductive hormone levels, namely Follicle Stimulating Hormone (FSH) and 17ß-estradiol levels in female white rats exposed to cigarette smoke. This study is an experimental study with Randomized Post Test Only Control Group Design, using 24 female white rats aged 3 months weighing 200-250 g divided into 4 groups, namely the control group (K), the cigarette smoke group (P1), the purslane leaf extract group. (P2) and the group of cigarette smoke accompanied by purslane leaf extract (P3). Ovarian MDA measurement is a measurement of MDA levels as an indicator of oxidative stress in the ovaries determined by the spectrophotometry method. Measurement of FSH and 17ß-estradiol levels in blood taken intracardially through the right ventricle of the heart as measured by the ELISA method. The results showed that purslane leaf extract at a dose of 200mg/KgBW reduced ovarian MDA levels of female white rats exposed to cigarette smoke significantly with p-value <0.05. However, there has been no significant change in FSH and 17ß-estradiol. Keywords: purslane leaf extract, cigarette smoke, Follicle Stimulating Hormone, 17ß-estradiol, malondialdehyde, health risk

scholarly journals Antioxidant Effect of the Castanea sativa Mill. Leaf Extract on Oxidative Stress Induced upon Human Spermatozoa

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Marco Biagi ◽  
Daria Noto ◽  
Maddalena Corsini ◽  
Giulia Baini ◽  
Daniela Cerretani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Sperm Motility ◽  
Leaf Extract ◽  
Colorimetric Assay ◽  
Castanea Sativa ◽  
High Concentration ◽  
Tem Analysis ◽  
Dpph Test

This study was aimed at evaluating in vitro the effects of a 75% v/v ethanolic extract of leaves of Castanea sativa Mill. (var. Bastarda Rossa, Mount Amiata, Tuscany, Italy) on ejaculated human sperm. Total polyphenols and total flavonoids contained in the extract were determined by a colorimetric assay and HPLC-DAD. The DPPH test and electrochemistry were utilized to study the antioxidant activity of the extract. Swim-up-selected sperm from 8 healthy men were treated with the C. sativa leaf extract at different dilutions (1 : 100, 1 : 200, and 1 : 500), and sperm motility was assessed following the WHO guidelines. Swim-up-selected spermatozoa were incubated with 100 μM H2O2 to induce lipid peroxidation (LPO) and with H2O2 and the leaf extract (1 : 100, 1 : 200, and 1 : 500) to test the antioxidant activity of the extract. The levels of LPO were determined by measuring malondialdehyde (MDA) concentrations. The treated samples were also analyzed by transmission electron microscopy (TEM) for ultrastructural evaluation. The chemical analysis showed that one-third ca. of the polyphenols in the C. sativa extract were made up of flavonoids, with hyperoside present in high concentration. A good antioxidant activity was demonstrated by both the DPPH test and electrochemical analysis. The C. sativa leaf extract did not decrease sperm motility at all tested dilutions. Treatment with H2O2 alone caused a significant increment in MDA levels (P=0.006993), while the treatment with H2O2 plus C. sativa extract diluted to 1 : 100 and 1 : 200 significantly reduced MDA levels (P=0.01476 and P=0.01571, respectively), with respect to H2O2 alone. TEM analysis confirmed the protective effect of the extract on damage induced by LPO, in particular that occurring at the plasma membrane level. The C. sativa leaf extract could be used in human and farm animal protocols for gamete handling, such as techniques of assisted reproduction and cryopreservation of semen, all conditions in which oxidative stress is exacerbated.

scholarly journals Amaranth Leaf Extract Protects Against Hydrogen Peroxide Induced Oxidative Stress in Drosophila Melanogaster

2021 ◽  
Author(s):  
Johnmark Ndinawe ◽  
Hellen W. Kinyi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Drosophila Melanogaster ◽  
Leaf Extract ◽  
Dependent Manner ◽  
Polyphenol Compounds ◽  
Dose Dependent

Abstract ObjectiveAmaranths leaves are rich in ascorbic acid and polyphenol compounds which have antioxidant activity. The aim of this study was to evaluate their in vivo antioxidant activity. The effect of consumption of Amaranth leaf extract on in vivo antioxidant activity, catalase enzyme activity and H2O2 induced oxidative stress in Drosophila melanogaster flies was assessed.ResultsConsumption of Amaranth leaf extract was associated with increased survival on exposure to H202 in a dose dependent manner in Drosophila melanogaster flies.

scholarly journals In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

2020 ◽  
pp. 48-60
Author(s):  
Udedi Stanley Chidi ◽  
Ani Onuabuchi Nnenna ◽  
Asogwa Kingsley Kelechi ◽  
Maduji Fitzcharles Chijindu ◽  
Okafor Clinton Nebolisa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Dpph Radical ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

scholarly journals Therapeutic Effect of Red Spinach (Amaranthus tricolor L.) Extract on Pancreatic MDA Levels Rats (Rattus norvegicus) Exposed to MLD-STZ

2021 ◽  
Vol 7 (3) ◽  
pp. 129-133
Author(s):  
Kartika Rahma ◽  
Ois Nurcahyanti
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Rattus Norvegicus ◽  
Amaranthus Tricolor ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Spinach Extract ◽  
Sugar Levels

Background: Several studies have reported that an increase in blood glucose in patients with diabetes mellitus (DM) can cause complications to death that occur due to oxidative stress conditions due to excessive ROS production. Red spinach extract was found to be rich in antioxidant compounds. However, there has been no further research on the ability of red spinach to lower blood glucose levels and prevent oxidative stress, which can be seen from the levels of malondialdehyde (MDA; a marker of oxidative stress) in the DM body.Objective: This study aims to determine whether there is a change in blood glucose levels and MDA levels in DM animal models between the groups that were given red spinach extract and not.Methods: This study used white rats (Rattus norvegicus) which were divided into 5 groups: K(-) group in which the rats were not induced by diabetogenic agent and were not treated; K(+) group, the rats were made DM induced by multiple low dose streptozotocin (MLD-STZ); and T1, T2, and T3 groups were exposed to MLD-STZ and treated with red spinach extract (Amaranthus tricolor L.) at a dose of 200 mg/kgBW, 300 mg/kgBW and 400 mg/kgBW, respectively. Blood sugar levels were checked before and after treatment with extract using glucometer digital. Meanwhile, MDA levels were measured by TBA test using protein isolates from the pancreas of each rat.Results: The results showed that the average MDA levels in the K-, K+, T1, T2, and T3 groups were 1.759±0.08, 2.280±0.15, 2.303±0.11, 1.927±0.06, and 1.801±0.04. While the average blood sugar levels in the K-, K+, T1, T2, and T3 groups were 114.4±8.82, 464±72.78, 421.2±37.60, 140.6±20.19, and 176±13.06.Conclusion: It can be concluded that the administration of red spinach extract therapy in DM model rats was able to reduce both glucose levels and MDA levels. By lowering the levels of MDA compounds, it is also believed to be able to prevent oxidative stress in cells which causes tissue damage.

scholarly journals Aktivitas Antioksidan Ekstrak Fenol Daun Gayam (Inocarpus fagiferus Fosb)

Biosfera ◽  
2018 ◽  
Vol 35 (1) ◽  
pp. 37
Author(s):  
Dwi Marga Lestari ◽  
Nurul Mahmudati ◽  
Sukarsono Sukarsono ◽  
Nurwidodo Nurwidodo ◽  
Husamah Husamah
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Assay Method ◽  
Total Phenolic ◽  
Powder Samples ◽  
Leaf Powder ◽  
Dark Green

This study aims to determine the total phenolic content and antioxidant activity in gayam leaf extract (Incarpus fagiferus Fobs). The research method used is a quasi-experiment that aims to predict the situation to be achieved through actual experiments but no treatment. The sample used is old gayam leaves, with the characteristic of dark green leaf and rough leaf surface. The process of preparing simplicia, ie preparing fresh gayam leaves, dried in an oven temperature 45-50oC, and then dried to produce gayam leaf powder. Samples were extracted with methanol solvent and ethanol for 5 days. The total phenol assay method uses Folin-Ciocalteau method and antioxidant activity test using DPPH free radical retardation method (1,1-diphenyl-2-picrylhydrazyl). The results showed that the total phenolic content of gayam leaf extract with ethanol and methanol solvent was 313,704 GAE (Gallic Acid Equivalent) and 273,913 GAE, respectively. Antioxidant activity as a free antidote to free radical DPPH is known to be valued with IC50 (inhibitory concentration).

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

scholarly journals Effect of Ethanol Extract of Mangosteen Peel on Serum Malondialdehyde and Leukotriene B4 Levels in Male White Rats (Rattus norvegicus) Wistar Strain Exposed with Electric Cigarette Smoke

2021 ◽  
Vol 56 (4) ◽  
pp. 283
Author(s):  
Jemima Lewi Santoso ◽  
Harianto Notopuro ◽  
Ema Qurnianingsih
Keyword(s):  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Leukotriene B4 ◽  
The Body ◽  
Control Group ◽  
Test Results ◽  
White Rats ◽  
Mangosteen Peel ◽  
Significant Difference

This study aims to analyze the administration of mangosteen peel ethanol extract at different doses can reduce levels of malondialdehyde (MDA) and leukotriene B4 (LTB4) serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke. Electric cigarette induces mitochondrial ROS production and triggers oxidative stress. Antioxidants are needed by the body if there are many free radicals, for example mangosteen peel. This laboratory experimental study uses a randomized post test only control group design. Thirty male white rats were divided into 5 groups randomly (KK, KR, P1, P2, P3). KK given 0.5% Na-CMC, KR given smoke and 0.5% Na-CMC, P1, P2, P3 given smoke and mangosteen peel ethanol extract 100, 200, 300 mg / kg weight. Data were tested for normality and homogeneity tests. Data distribution was found not normal. Data were tested by Kruskal-Wallis Test and Mann-Whitney. Kruskal-Wallis Test results showed significant differences in MDA and LTB4 levels in each experimental group (KS, KR, P1, P2 and P3), p <0.05. Mann-Whitney test results showed a significant difference in the MDA levels of the KR against P3 and in the LTB4 level of the KR against P2 (p <0.05). The conclusion of the study was the administration of mangosteen peel ethanol extract can reduce MDA and LTB4 levels serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke.

scholarly journals Antioxidant Activity of an Aqueous Leaf Extract from Uncaria tomentosa and Its Major Alkaloids Mitraphylline and Isomitraphylline in Caenorhabditis elegans

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3299 ◽  
Author(s):  
Bruna C. Azevedo ◽  
Mariana Roxo ◽  
Marcos C. Borges ◽  
Herbenya Peixoto ◽  
Eduardo J. Crevelin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Transcription Factor ◽  
Caenorhabditis Elegans ◽  
Leaf Extract ◽  
Therapeutic Potential ◽  
Lethal Dose ◽  
Uncaria Tomentosa ◽  
Aqueous Leaf Extract

Uncaria tomentosa (Rubiaceae) has a recognized therapeutic potential against various diseases associated with oxidative stress. The aim of this research was to evaluate the antioxidant potential of an aqueous leaf extract (ALE) from U. tomentosa, and its major alkaloids mitraphylline and isomitraphylline. The antioxidant activity of ALE was investigated in vitro using standard assays (DPPH, ABTS and  FRAP), while the in vivo activity and mode of action were studied using Caenorhabditis elegans as a model organism. The purified alkaloids did not exhibit antioxidant effects in vivo. ALE reduced the accumulation of reactive oxygen species (ROS) in wild-type worms, and was able to rescue the worms from a lethal dose of the pro-oxidant juglone. The ALE treatment led to a decreased expression of the oxidative stress response related genes sod-3, gst-4, and hsp-16.2. The treatment of mutant worms lacking the DAF-16 transcription factor with ALE resulted in a significant reduction of ROS levels. Contrarily, the extract had a pro-oxidant effect in the worms lacking the SKN-1 transcription factor. Our results suggest that the antioxidant activity of ALE in C. elegans is independent of its alkaloid content, and that SKN-1 is required for ALE-mediated stress resistance.

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