scholarly journals Sublethal Effects of Organophosphate Chlorpyrifos on Hemato-Immunological Parameters of the Gercacinid Crab, Cardiosoma armatum (Herklots, 1851)

Bio-Research ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 1185-1191
Author(s):  
Christian Chinonso Ugwu ◽  
Rasheed Olatunji Moruf ◽  
Aderonke Omolara Lawal-Are
Keyword(s):  
Sedimentation Rate ◽  
Significant Variation ◽  
Biochemical Parameters ◽  
Sublethal Effects ◽  
Hematological Parameters ◽  
Serum Biochemistry ◽  
Control Group ◽  
Immunological Parameters ◽  
Insecticide Exposure ◽  
Serum Protein Level

Low insecticide exposure has been shown to cause profound effects on non-target organisms, including crabs. Therefore, the changes in hematological parameters, serum biochemistry and antioxidant enzymes in the Gercacinid crab, Cardiosoma armatum were assessed during 28-day exposure to four concentrations of organophosphate chlorpyrifos (0.003, 0.006, 0.03 and 0.06 mg/l). The results showed a significant (P= 0.0) decrease in packed cell volume and total haemocyte count of the exposure crabs (except in 0.003 mg/l concentration) compared to control group. There were no significant changes in hemocyte sedimentation rate, granulocyte and agranulocyte, although all exposure groups increased in hemocyte sedimentation rate and agranulocyte with respective ranges of 3.00-3.02 mm/hr and 64.00-67.00 %. Except for alkaline phosphatase, there were no significant variation in the biochemical profile of both the control crabs and exposure crabs, although organophosphate chlorpyrifos exposure induced increase in all the measured biochemical parameters. The serum protein level and the activities of the enzymes (superoxide dismutase, catalase and malondialdehyde) were inhibited in exposure groups. The changes in these hemato-immunological parameters of the crabs were suitable biomarkers of a sub-lethal exposure to chlorpyrifos at the concentrations tested, and this will be useful in biomonitoring of aquatic environment.

scholarly journals Haematological parameters in patients with rheumatoid arthritis and gene variants HLA-DRB1 * 04 and HLA-DRB1 * 03

2020 ◽  
Vol 4 (1) ◽  
pp. 30
Author(s):  
Biljana Klimenta ◽  
Hilada Nefić ◽  
Nenad Prodanović ◽  
Fatima Hukić ◽  
Aner Mešić
Keyword(s):  
Rheumatoid Arthritis ◽  
Sedimentation Rate ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Unknown Etiology ◽  
Control Group ◽  
Gene Variants ◽  
Underdeveloped Countries ◽  
Erythrocyte Sedimentation ◽  
Hematological And Biochemical Parameters

Rheumatoid arthritis is a polygenic disease of unknown etiology, occurs worldwide in both developed and underdeveloped countries and involves all races. The aim of this study is to determine the correlation between hematological parameters (DBC and ESR) and biomarkers of inflammation (CRP) in patients with RA predisposing gene variants HLA-DRB1*04 or HLA-DRB1*03. This study analyzed the results of hematological and biochemical parameters of 33 patients diagnosed with RA, carriers ofgene variants of HLA-DRB1*04 or HLA-DRB1*03, and 33 subjects of control group non-carriers for HLA-DRB1*04 or HLA-DRB1*03. All hematological parameters (DBC) were analyzed on a Beckman Coulter DxH 800 hematology counter. The erythrocyte sedimentation rate was expressed in mm/h. The CRP biochemical test was performed on a Cobas c311 automatic analyzer. In group of RA patients carriers of HLA-DRB1*04 or HLA-DRB1*03 gene variants, the values of HGB and HCTwere significantlylower(p < 0.05) while the values of RDW, RDW-SD, MO, BA, MO#, BA#, ESR and CRP were statistically increased (p < 0.05) from the control group without these variants.

scholarly journals Comparative Effects of Using New Multi-Strain Synbiotics on Chicken Growth Performance, Hematology, Serum Biochemistry and Immunity

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1555
Author(s):  
Artur Żbikowski ◽  
Karol Pawłowski ◽  
Katarzyna Śliżewska ◽  
Beata Dolka ◽  
Joanna Nerc ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Serum Biochemistry ◽  
Control Group ◽  
Normal Ranges ◽  
Rbc Count

In this study, the influence of new multi-strain synbiotics on chicken growth performance, hematology, serum biochemistry and immunity was explored. Each synbiotic preparation (A, B and C) comprised three, four or five strains of Lactobacillus sp., respectively, as well as S. cerevisiae and inulin. All strains used in the synbiotics originated from wild-type strains from animal farms in Poland. Six groups of chickens, ROSS 308 line, were fed with three different synbiotics at a dose of 0.5 g/1 kg of feed. Body weight, as well as the biochemical and hematological parameters of the animals in each study group, were determined on the 7th, 14th, 28th and 42nd day of life. Body weight on day 42 differed between groups and was the highest in control group. This group also had the highestfeed conversion ratio (FCR) value. All measured biochemical parameters were in the normal ranges for poultry; however, we observed a lower alkaline phosphatase (AP) concentration on day 7 in the groups fed with synbiotics, which correlated with a lower level of triglycerides in those groups. The aspartate transaminase (AST) concentration was significantly lower in all groups on day 42 in comparison with the control. On day 7, the control group showed the highest concentration of Ca, K and P. Other parameters did not differ significantly throughout the experiment. All groups showed a similar tendency of increase in the red blood cells (RBC) count according to the age of the birds. Every white blood cells (WBC) population showed differences in the proportions between T and B lymphocytes. The T cell and monocyte counts increased until day 28 in all groups. The results showed that our newly developed synbiotic formulas do not have any unfavorable influence on chicken health and may modulate immune response and biochemical parameters. However, this hypothesis needs to be evaluated in future experiments.

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

scholarly journals Influence of Pesticide on Biochemical and Hematological Parameters in Beninese Vegetable Farmers

2018 ◽  
Vol 9 (1) ◽  
pp. 65
Author(s):  
Lucie AYI-FANOU
Keyword(s):  
Blood Cell ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Study Group ◽  
Blood Samples ◽  
Vegetable Farmers ◽  
Insignificant Difference ◽  
Hematological And Biochemical Parameters

In several countries, a lot of researches have shown the toxicity of pesticides on farmers. Meanwhile, few of them have dealt with vegetable farmers in Benin. Therefore, this study has been carried out to assess the effects of pesticides on vegetable farmers’ health. This study is conducted on 30 vegetable farmers who used pesticides and 20 subjects as control group. Pesticides used by vegetable farmers and their risky behavior were investigated. Their blood samples were collected and some tests were performed for hematological and biochemical parameters. For the statistical analysis of the results, Student’s test was used. Our investigations revealed that vegetable farmers of Benin were exposed to different active ingredients of pesticides such as (Abamectin, Acetamiprid, Chlorpyriphos ethyl, Cyfluthrin, Cypermethrin, Emamectin benzoat, Flubendiamid, Lambdacyhalothrin, Mancozeb, Profenofos, Spinosad and Spirotétramate). The hematological parameters showed significant decrease in White blood cell (WBC), Red blood cell (RBC), Hemoglobin (HGB), Hematocrit (HTC), Mean Corpuscular Volume (MCV), Platelet (PLT) count among study group. The enzymatic activities of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) have significantly raised between vegetable farmers and the control group, while the serum concentration in urea and creatinine showed insignificant difference in the study group. These results suggest that pesticides have adverse effects on vegetable farmers of South of Benin.

scholarly journals A 90-Day Oral Toxicity of Hydroethanolic Root Extract of Carissa spinarum in Wistar Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Komlan M. Dossou-Yovo ◽  
Aboudoulatif Diallo ◽  
Povi Lawson-Evi ◽  
Yendubé T. Kantati ◽  
Tchin Darré ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Relative Weight ◽  
Control Group ◽  
Root Extract ◽  
Weight Changes ◽  
Oral Toxicity ◽  
Significant Difference

Background. Herbal medication is a worldwide and ancient practice, mostly in developing countries, where a large part of the population is involved in this practice. Hence, studies must be conducted to evaluate their safety and efficiency to avoid or prevent toxicological risks due to their usage. In Togo, Carissa spinarum is a medicinal plant belonging to Apocynaceae family, used as an aphrodisiac or to heal some ailments including malaria, sickle cell anemia, hypertension, pain, and asthma. Notwithstanding its several ethnomedicinal benefits, just a few toxicological data associated with its chronic use are available. Objective. Therefore, this study aims to assess the toxicity of an ethanolic root extract of Carissa spinarum in Wistar rats. Methods. The 90-day oral toxicity process following OECD TG 408 guidelines is used. Male Wistar rats received Carissa spinarum root hydroethanolic extract at 500 and 1000 mg/kg for 90 days by oral gavage. Body weight changes, hematological and blood biochemical parameters, organ weight changes, malondialdehyde as a lipoperoxidation marker expressed according to tissue proteins, and histopathology of vital organs were assessed. Results. No signs of toxicity or mortality were observed during the 90 days experiment. Hematological parameters have not shown any treatment-related abnormalities. According to biochemical parameters, an increase in the chloride ion level was observed at 1000 mg/kg p < 0.01 . There was no significant difference between the treated groups and the control group concerning the malondialdehyde concentration, body weight, and organ relative weight. No changes in necropsy and histopathology of vital organs associated with extract treatment were observed. Conclusion. The results indicated that an ethanolic root extract of Carissa spinarum does not cause adverse effects, which can lead to Wistar rats’ death after 90-day oral administration at 500 and 1000 mg.

scholarly journals Acute and sublethal intoxication of malathion in an Indian major carp, Labeo rohita: haematological and biochemical responses.

2021 ◽  
Vol 36 (3) ◽  
pp. e2021016
Author(s):  
Juginu Mankuzhiyil Sivanandan ◽  
Binukumari
Keyword(s):  
Sublethal Effects ◽  
Labeo Rohita ◽  
Hematological Parameters ◽  
Organophosphorus Pesticide ◽  
Control Group ◽  
Indian Major Carp ◽  
Biochemical Responses ◽  
Lc50 Value ◽  
Hematological And Biochemical Parameters ◽  
The Impact

This study aimed to determine the median lethal concentration (96-h LC50), acute and sublethal effects of malathion, an organophosphorus pesticide on hematological and biochemical responses in an Indian major carp, Labeo rohita. In this study, the LC50 value of malathion for 96 h was found to be 3.4 ppm. During acute (3.4 ppm) and sublethal [1/10th of 96 h LC50 value (0.34 ppm) studies, all the hematological parameters except WBC were significantly decreased (p<0.05). Besides, when compared with the control group, a significant (p<0.05) decrease in biochemical activity was also observed in malathion treated fish during acute and sublethal exposure periods. These results suggest that the tested concentrations of malathion could have significant adverse effects on the hematological and biochemical parameters of fish, Labeo rohita. The changes in the parameters can be effectively used to determine the impact of malathion in the aquatic ecosystem.

scholarly journals Assessment of Toxicity and Therapeutic Effects of Goose Bone in a Rat Model

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Md. Yousuf Ali ◽  
Alamgir Kabir ◽  
Shahad Saif Khandker ◽  
Tareq Hossan ◽  
Md. Salman Shakil ◽  
...  
Keyword(s):  
Rat Model ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Atherogenic Index ◽  
Control Group ◽  
Histopathological Study ◽  
Therapeutic Effects ◽  
Related Disorder ◽  
Serum Biochemical ◽  
Hematological And Biochemical Parameters

Goose bone is traditionally used in the treatment of many ailments including in bone fracture. The aim of the present study was to evaluate the subacute toxicity of goose bone in a rat model by investigating some hematological and biochemical parameters in rats. Subsequently, a histopathological study was performed to confirm the presence of pathological lesions in the rat’s vital organs including the liver, kidney, heart, brain, pancreas, lung, spleen, and stomach. Adult Wistar rats were divided into four groups (n = 8) and were orally administrated with three doses (30, 60, and 120 mg/kg) of goose bone once daily for 21 days as compared to control animals (received only drinking water). Goose bone did not cause any significant changes on body weight, relative organ weight, and percentage water content at any of the administered doses. There were also no significant alterations in hematological parameters seen. All three doses administered significantly reduced the triglyceride levels as well as the atherogenic index of plasma (AIP). Animals treated with 120 mg/kg doses had significantly reduced alkaline phosphatase (ALP) activity as compared to the control group. There was no significant alteration on other serum biochemical parameters seen. Additionally, histopathological findings confirmed that there was no inflammatory, necrotic, or other toxicological feature seen for all three doses. It is concluded that goose bone is nontoxic and is safe for consumption besides having the potential to be investigated for the treatment of high triglycerides or liver-related disorder.

scholarly journals Effect of Carthamus Tinctorius Safflower Aqueous Extract Against Nickel Chloride Induces Hematotoxicity and Immunotoxicity in Adult Male Rabbits

2017 ◽  
Vol 30 (3) ◽  
pp. 19
Author(s):  
Layla Abd-Al-Sattar Sadiq Laylani
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Nickel Chloride ◽  
Carthamus Tinctorius ◽  
Stress Factors ◽  
Control Group ◽  
Immunological Parameters ◽  
And Oxidative Stress

  This study was designed to show, the role of Carthamus tinctorius safflower aqueous extract against toxicity of nickel chloride (NiCl2). Twenty male, rabbits were used and divided into four groups (with 5 rabbits in each group); group (control group) received normal diet, group II received orally 100mg/kg NiCl2 for six weeks, group III received 100mg/kg NiCl2 and 100mg/kg extract six weeks, group IV received 100mg/kg NiCl2 and 200mg/ kg extract six weeks. Hematological parameters showed (RBC (Red blood cells), Hb (Hemoglobin), PCV (Packed cells volume) decreased and WBC (White blood cells) increased) significant changes (P < 0.05) compared with control group. Immunological parameters (IgG, IgA and IgM increased) and oxidative stress factors (MDA increased and GSH decreased) show significant changes (P < 0.05) compared with control group. While, safflower aqueous adverse the negative effects of NiCl2 and causing ameliorative effects on all hematological parameters, hematological immunological parameters and oxidative stress factors showed no significant changes (P < 0.05) compared with control group. It was concluded that flower extract of Carthamus tinctorius has been antioxidant role against nickel chloride toxicity in rabbits.  

scholarly journals The effect of the insect-acaricidal drug Delcid 7.5® on the body of rabbits

2021 ◽  
Author(s):  
M.I. Burmistrova ◽  
F.I. Vasilevich ◽  
A.A. Deltsov
Keyword(s):  
Peripheral Blood ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Spinal Column ◽  
The Body ◽  
Control Group ◽  
Toxicological Effect ◽  
Hematological And Biochemical Parameters ◽  
External Stimuli ◽  
Average Hemoglobin

The effect of the drug Delcid 7.5 ® on the clinical, hematological and biochemical parameters of rabbits of the New Zealand breed, 6 months of age, when used in doses of 2 ml or 4 ml. Three times with a cutaneous application along the vertebral column from the head to the tail. The conducted studies allow us to conclude that after applying the drug in these doses for 3 days, there were no external signs of intoxication in rabbits. All the animals of the experimental groups were active. The reaction to external stimuli is preserved. Hematological parameters of peripheral blood (number of erythrocytes, leukocytes, hemoglobin, color index, the average hemoglobin content of PG in erythrocytes, mean corpuscular volume, the exponent of anisocytosis of red blood cells, etc.) of the experimental group after treatment in 5, 15, 25 days had no significant differences from those in the control group, this suggests that the applied drug to the skin along the spine from the withers to the rump at a dose of 2 and 4 ml has no Toxicological effect on peripheral blood. The leukogram of the peripheral blood of rabbits when using the drug did not differ from the control parameters. The analysis of biochemical parameters (activity of aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatinine, urea, glucose, total protein, bilirubin, etc.) of the blood of the experimental groups when applying the drug Delcid 7.5 ® along the spinal column at the maximum therapeutic dose did not reveal statistically significant differences from the control, which indicates that the drug does not have a genotoxic effect, does not interfere with the kidneys and metabolism in the body of rabbits. The results of the conducted studies show that the insecticide-acaricide drug Delcid 7.5® for veterinary use, developed by NVC Agrovetzashchita LLC, is safe for rabbits, and therefore it can be recommended for studying the effectiveness of acarosis in rabbits and other animal species.

Sign in / Sign up

Export Citation Format

Share Document