THE ANATOMICAL-MORPHOLOGICAL FEATURES OF OREGANO ORDINARY ($ORIGANUM~VULGARE~\mathrm{L.}$) WIDLY GROWING IN ARMENIAN FLORA DURING THE PRE-BLOSSOMING PERIOD

2021 ◽  
Vol 55 (1 (254)) ◽  
pp. 46-51
Author(s):  
Armenuhi V. Moghrovyan
Keyword(s):  
Essential Oil ◽  
Raw Materials ◽  
Climatic Conditions ◽  
Morphological Features ◽  
High Density ◽  
Origanum Vulgare ◽  
Oil Glands ◽  
Capitate Trichomes ◽  
Microscopic Studies ◽  
Climatic Features

The article reports the results of microscopic studies of the oregano leaf in the pre-blossom period. In particular, the following anatomical and morphological features of the oregano leaf during the pre-blossom period were revealed: sinuous cuticle, multicellular simple trichomes, capitate trichomes, stomata, essential oil glands. The latter were located only in the central part, and were absent at the margins. The essential oil glands were of high density mostly in the central part of the raw materials preparation, which were harvested from the regions of dry climatic conditions as opposed to the raw materials harvested from the region of wet climatic conditions. The results of the investigation were definitely conditioned by the climatic features of the regions.

STUDY OF MICROSCOPIC FEATURES OF TANACETUM SANTOLINA PLANT

2022 ◽  
pp. 38-44
Author(s):  
М.С. Жанибек ◽  
К.К. Орынбасарова
Keyword(s):  
Essential Oil ◽  
Calcium Oxalate ◽  
Raw Materials ◽  
Microscopic Analysis ◽  
Anatomical Structure ◽  
Raw Material ◽  
Plant Fibers ◽  
Oil Glands ◽  
Plant Raw Materials ◽  
Diagnostic Signs

В данной статье представлены результаты микроскопического исследования особенностей анатомического строения (листья, цветки) растения пижмы сантолинной (Tanacetum Santolina). Цель работы: изучение анатомического строения надземных органов, определение подлинности и отличия от других видов. Материалы и методы: объект исследования - цветки пижмы сантолинной, собранные в период цветения в мае 2020 года в предгорных равнинах Каратау. Микроскопическое исследование цветков пижмы сантолинной проводилось в соответствии с требованиями государственной фармакопеи Республики Казахстан. С помощью микроскопа Биолам-5 изучался объект с увеличением от х40 до х400. Исследование срезов и создание микрорисунков проводили с помощью цифрового камерного тринокулярного микроскопа «МТ300L» (Япония) (увеличенный 7х1,5х4,5; 7х1,5х8; 7х1,5х40). Результаты и обсуждения: изучены диагностические признаки для определения цельного сырья: диагностические признаки листьев, форма клеток эпидермиса, тип аппарата устьиц, строение волосков и эфирномасличных желез; формы оксалата кальция. Выводы: проведен микроскопический анализ цветков пижмы сантолинной. Для определения подлинности этого сырья были выявлены анатомодиагностические признаки. Во всех частях растения встречаются одноклеточные волоски и пучковые волокна. На обеих частях листовой пластины хорошо видны волокна и волоски, устьица, пигментные полости. Кристаллы оксалата кальция, находятся в мезофильных полостях листа. В цветках оксалат кальция встречается только в виде четырехугольного кристалла. На анатомическом признаке измельченного сырья видны эфирномасличные железки. Форма эфирно-масличных желез обнаруживает основные диагностические признаки цветков и листьев пижмы сантолинной. Анатомические характеристики позволяют повысить уровень стандартизации, дальнейшее изучение лекарственного растительного сырья This article presents the results of a microscopic study of the anatomical structure (leaves, flowers) of the Santolina’s tansy plant (Tanacetum Santolina). The purpose of the work: to study the anatomical structure of aerial organs, to determine authenticity and differences from other species. Materials and methods: the object of research is the flowers of Santolina’s tansy collected during the flowering period in May 2020 in the foothill plains of Karatau. Microscopic examination of the flowers of Santolina’s tansy was carried out in accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan. Using a Biolam-5 microscope with magnification from x40 to x400 an object was studied. The study of sections and the creation of micro-nozzles was carried out using a digital chamber trinocular microscope "MT300L" (Japan) (enlarged 7x1.5x4.5; 7x1.5x8; 7x1.5x40). Results and discussions: diagnostic signs for the determination of whole raw materials were studied: diagnostic signs of leaves, the shape of epidermis cells, the type of stomatal apparatus, the structure of hairs and essential oil glands; forms of calcium oxalate. Conclusions: microscopic analysis of flowers of Santolina’s tansy was carried out. Anatomical and diagnostic signs were identified to determine the authenticity of this raw material. Unicellular hairs and beam fibers are found in all parts of the plant. Fibers and hairs, stomata, pigment cavities are clearly visible on both parts of the leaf plate. Calcium oxalate crystals are located in the mesophilic cavities of the leaf. In flowers, calcium oxalate occurs only in the form of a quadrangular crystal. On the anatomical feature of the crushed raw materials, essential oil glands are visible. The shape of the essential oil glands reveals the main diagnostic signs of flowers and leaves of santolina tansy. Anatomical characteristics make it possible to increase the level of standardization, further study of medicinal plant raw materials.

scholarly journals Pemuliaan Tanaman Nilam (Progestemon cablin) Lokal Melalui Perkembangbiakan Vegetatif

2016 ◽  
Vol 18 (2) ◽  
pp. 123
Author(s):  
Hermin Pancasakti Kusumaningrum ◽  
Endang Dwi Purbajanti ◽  
Agus Setiadi
Keyword(s):  
Essential Oil ◽  
Vegetative Propagation ◽  
Rainy Season ◽  
Raw Materials ◽  
Climatic Conditions ◽  
Dry Season ◽  
The Other ◽  
Surrounding Area ◽  
Local Varieties ◽  
Different Seasons

Patchouli essential oil is an dominant product from Batang but its production has not been able to meet export needs. The main problem in the production of essential oil of patchouli is limited amount of seeds and dry season effect on the growth of patchouli. On the other hand, society units of patchouli essential oil is very dependent on the availability of raw materials  of patchouli. Searching of patchouli local varieties must  develop in order to  encourages of sustainibility of patchouli production. The purpose of this activity is search and propagate patchouli plant from local varieties that can survive in different seasons of the year according to the climatic conditions of Batang. The study was conducted with the search and propagation of patchouli plant varieties used by farmers in Batang which is Sidikalang varieties and local varieties originating from Batang region and the surrounding area. Furthermore, the vegetative propagation using cuttings will be use followed by its growth observation during dry and rainy season. Results showed that the activities of patchouli plant local varieties of Bandungan, Kendal and Batang showed the best growth in the dry season. Key words: patchouli, vegetatif growth, essential oil.

scholarly journals VARIABILITY OF COMPONENT COMPOSITION OF MELISSA OFFICINALIS L. ESSENTIAL OIL DEPENDING ON THE CLIMATIC CONDITIONS OF THE REGION OF CULTIVATION

2020 ◽  
pp. 257-263
Author(s):  
Natal'ya Vladimirovna Nevkrytaya ◽  
Vladimir Stepanovich Pashtetskiy ◽  
Ilya Aleksandrovich Novikov ◽  
Irina Nikolaevna Korotkikh ◽  
Ruslan Ramazanovich Tkhaganov
Keyword(s):  
Essential Oil ◽  
Raw Materials ◽  
Weather Conditions ◽  
Component Composition ◽  
Climatic Conditions ◽  
Melissa Officinalis ◽  
Oil Accumulation ◽  
Temperature And Precipitation ◽  
Air Temperatures ◽  
The Crimea

The aim of the research was to detect the dependence of content and component composition of Melissa officinalis L. essential oil from climatic peculiarities of the region of cultivation and to specify the optimum conditions for growing high-quality raw materials. We presented the results of the comparative assessment of essential oil component composition (three main elements) obtained from air-dried raw materials of two cultivars Krymchanka and Lada. These Melissa officinalis L. cultivars were grown in three regions that sharply differed in weather conditions. To make the comparison accurate, we determined the component composition of essential oil in the Laboratory of Biochemistry at FSBSI “Research Institute of Agriculture of Crimea” using gas chromatograph Kristall 5000.2. The most favorable climatic conditions for essential oil accumulation were in the Foothill zone of the Crimea (moderate rainfalls combined with high air temperatures). Cultivar Krymchanka exceeded cultivar Lada by the amount of essential oil in raw materials. Excess sometimes reached 170%. Dependence of the main components such as citral, β-caryophyllene, caryophyllene oxide, germacrene D from the ratio of temperature and precipitation was revealed. After obtaining research results, we could suggest that by controlling the conditions of cultivation of M. officinalis, it is possible to adjust the ratio of the components of essential oil.

scholarly journals Epidermal structures of leaves in some Mentha х piperita L. varieties in connection with they productivity

2019 ◽  
pp. 67-71
Author(s):  
E. L. Malankina ◽  
L. N. Kozlovskaya ◽  
E. N. Tkatcheva
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Raw Materials ◽  
Intraspecific Variability ◽  
Botanical Garden ◽  
Weight Percent ◽  
Medicinal And Aromatic Plants ◽  
Oil Glands ◽  
Mentha X Piperita ◽  
Wide Range

Relevance. It is known that peppermint (Mentha х piperita L.) is characterized by significant polymorphism, due to both genetic factors and growing conditions. Cultivated varieties and populations are distinguished by their economically significant characteristics, such as yield, winter hardiness, resistance to diseases, the content and composition of essential oil. Identification of new highly productive varieties and populations of Mentha x piperita L., distinguished by high productivity of essential oil in the Non-chernozem zone of the Russian Federation, as well as identification of morphological features characteristic of highly productive varieties, remains relevant.Methods. The object of the study was plants of 8 varieties of 2 year old plants and samples of Mentha x piperita L. from the collection of the SI Botanical Garden Rostovtsev RGAU-Moscow Agricultural Academy named after K.A. Timiryazev and from the collection of the Botanical Garden of All-Russian Institute of Medicinal and Aromatic Plants (VILAR), were used as the object of the study. Peppermint leaves (FS.2.5.0029.15 Peppermint leaves) and essential oil (GOST R 53593-2009) are used as herbal medicinal products. Microscopy used Primo Star Carl Zeiss light microscopes and LOMO MIKMED-1. Quantitative determination of the essential oil was carried out by distillation with water vapor, followed by measuring the volume of the resulting oil (GF RF XIV). The oil content was expressed in volumetric-weight percent in terms of dry raw materials.Results. As a result of a comparative study of the epidermal structures of the leaves of plants of 8 varieties of Mentha х piperita L., the density of stomata, the type of stomatal apparatus, density of location and length of multicellular trichomes, density and diameter of essential oil glands, and content of essential oil were determined. The variety specificity and variation of these indices are noted over a wide range, which is explained by the significant intraspecific variability characteristic of the genus Mint (Mentha L.). The most promising varieties of essential oil content were identified. 

scholarly journals Quality Variation of the Moldovan Origanum vulgare L. ssp. vulgare L. and Origanum vulgare L. ssp. hirtum (Link) Ietsw. Varieties in Drought Conditions

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1211
Author(s):  
Maria Gonceariuc ◽  
Mircea Valentin Muntean ◽  
Violeta Butnaraş ◽  
Marcel Matei Duda ◽  
Anna Benea ◽  
...  
Keyword(s):  
Essential Oil ◽  
Plant Protection ◽  
Climatic Conditions ◽  
The Other ◽  
Origanum Vulgare ◽  
Polyphenol Content ◽  
Drought Conditions ◽  
Polyphenolic Content ◽  
Quality Variation ◽  
Selection Of

In this paper, we have comparatively analyzed two subspecies of Origanum vulgare (O. vulgare ssp. hirtum and O. vulgare ssp. vulgare) in climatic conditions with higher than usual temperatures from the Institute of Genetics, Physiology and Plant Protection Chisinau, Rep. Moldova. The aim of the research was to study the producing capacity, content, composition of essential oil, as well as the polyphenols content in O. vulgare varieties, their drought testing and the selection of promising cultivars. Seven O. vulgare ssp. vulgare (Ov) varieties and fourteen O. vulgare ssp. hirtum (Oh) varieties were used as the biological material. The essential oil (EO) separated by hydrodistillation was analyzed by GC-MS techniques. The polyphenolic content was assessed using spectrophotometric techniques. The presence of a direct correlation between the content, production of essential oil and content of polyphenols has been highlighted. Thus, the varieties of O. vulgare ssp. hirtum demonstrate a higher content of essential oil than the varieties of O. vulgare ssp. vulgare, while the polyphenol content, on the other hand, is higher for the varieties of O. vulgare ssp. vulgare than for those of O. vulgare ssp. hirtum. This research has resulted in the selection of two varieties, i.e., “Savoare” of O. vulgare ssp. hirtum and “Panacea” of O. vulgare ssp. vulgare.

scholarly journals Can Glandular Hair Density be a Breeding Marker for Origanum Vulgare subsp. Hirtum with High Essential Oil Content?

2010 ◽  
Vol 5 (9) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Krisztina Szabó ◽  
Szilvia Sárosi ◽  
Beatrix Cserháti ◽  
Antal Ferenczy
Keyword(s):  
Essential Oil ◽  
Plant Material ◽  
Oil Content ◽  
Morphological Characteristics ◽  
Glandular Hair ◽  
High Ratio ◽  
Morphological Features ◽  
Origanum Vulgare ◽  
Hair Density ◽  
Morphological Marker

Origanum vulgare subsp. hirtum (Link) Ietswaart is an essential oil rich plant traditionally used as oregano. Based on the interest of the essential oil producing sector, in 2000 we have started a breeding program of O. vulgare subsp. hirtum. Plant material for our breeding work consists of 6 progeny. Individual evaluation of the plant material was carried out in 2008-2009 with the primary aim of finding mother plants with appropriate morphological features, high essential oil content (>7%) and with carvacrol as the main essential oil component. Among the survey of morphological characteristics special attention was given to glandular hair density in order to test the usability of it as a morphological marker for screening progeny for high essential oil content. The characteristics of the progeny can be described with high variability ensuring the possibility of a good selection base. Evaluating the morphology, essential oil content and constitution of the individuals, 20 plants were selected on the grounds of their high (7-8.6%) essential oil content, high ratio (70-93%) of carvacrol in the essential oil and typical morphological features of O. vulgare subsp. hirtum. From the results of glandular hair density it can be stated that the correlation between glandular hair density of the upper, middle and lower leaves either on vegetative or generative shoots and essential oil content was never strong enough (correlation coefficient ≤ 0.5) to use it exclusively as a morphological marker for individual selection.

scholarly journals BIOLOGICALLY ACTIVE SUBSTANCES EXHIBITING ANTIOXIDANT ACTIVITY, SOME REPRESENTATIVES OF THE LAMIACEAE FAMILY CULTIVATED IN THE STAVROPOL REGION

2019 ◽  
pp. 163-172
Author(s):  
Ivan Viktorovich Popov ◽  
Vera Vladimirovna Chumakova ◽  
Ol'ga Ivanovna Popova ◽  
Valeriy Fedorovich Chumakov
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Essential Oil ◽  
Raw Materials ◽  
Salvia Officinalis ◽  
Origanum Vulgare ◽  
Salvia Sclarea ◽  
The Family ◽  
Stavropol Region ◽  
Hyssopus Officinalis

The object of the study was the samples of raw materials of the family Lamiaceae plants created in FSBSI "North Caucasus Federal agricultural research center", using a variety of breeding methods: Salvia Officinalis Leaves (cultivar "Dobrynya"), Origanum Vulgare Herb (cultivar "Karamelka"), Salvia Sclarea Herb (cultivar "Salyut"), Satureja Hortensis Herb (cultivar "Karapuz"), Lophanthus Anisatus Herb (cultivar "Premier"), Hyssopus Officinalis Herb (cultivar "Rozovy flamingo"). As a result of the studies conducted by the method of high-performance liquid chromatography, the component composition of phenolic compounds (flavonoids and phenolcarbonic acids) was established. The quantitative content of essential oil, flavonoids and tannins was determined in the studied objects. In salvia officinalis leaves identified 11 phenolic compounds, 2.72% essential oil, 1.22% flavonoids, 12.20% tannins; in origanum vulgare herb – 9 compounds, 1.80% essential oil, 2.10% flavonoids, 8.64% tannins; in salvia sclarea herb – 11 compounds, 0.45% essential oil, 2.25% flavonoids, 10.51% tannins; in satureja hortensis herb – 11 compounds, 0.68% essential oil, 0.85% flavonoids, 9.37% tannins; in lophanthus anisatus herb – 11 compounds, 2.15% essential oil, 2.06% flavonoids, 8.30% tannins; in hyssopus officinalis herb – 9 compounds, 0.78% essential oil, 0.91% flavonoids, 9.55% tannins. Determination of antioxidant activity, conducted in two ways (amperometric and titrimetric) showed that water-alcohol extraction of plant raw materials of new varieties of representatives of the family Lamiaceae, created and maximally adapted to the conditions of Stavropol region can be the basis for the production of herbal remedies with antioxidant activity.

The basis of quality of sheep pelts and fur products is morphological features of the skin of sheep

2020 ◽  
pp. 50-57
Author(s):  
I. Dmitrik ◽  
G. Zavgorodnyaya
Keyword(s):  
Mechanical Strength ◽  
Breaking Load ◽  
Hair Follicles ◽  
Morphological Features ◽  
High Density ◽  
Fat Cells ◽  
High Quality ◽  
Histological Features ◽  
Weakened Zone

The morphological and histological features of the skin and wool cover of sheep as the basis for the quality of fur sheep pelts have been studied. The most important properties of sheep pelts (uniformity, thinness and density of wool) are provide the possibility of producing high-quality fur semi-finished products from them. However, the features of the histostructure of fine-wool sheep determine the low mechanical strength of the “facial” layer of skin. As a result, the “front” layer during processing often cracks to the upper border of the reticular layer or even peels off from the latter, making the sheep pelt unsuitable for use on fur products. These defects in fur practice are called “cracking” and “peeling” of the facial layer. They are mainly peculiar to sheep pelts of fine-wooled sheep. In these animals due to the high density and tone of the coat, the roots and hair follicles, root vaginas, secretory departments, excretory ducts of the glands and other structures occupy a significant share of the volume in the thickness of the Pilar layer (up to 25–30 %). The share of fibrous structures remains less volume, and these structures themselves are relatively weakly developed, located loosely and loosely intertwined with each other. The accumulations of fat cells that occur here also cannot be attributed to skin-strengthening elements. In fine-fleece sheep the pilar layer is on average 60 % of the thickness of the dermis. Therefore, more than half of its thickness is a weakened zone. The strength of the “front” layer is not the same in different fine-wool breeds of sheep and in different animals within the breed. For example, the average breaking load for cod of the “front” layer in Soviet Merino pelts is 1,25 kg, and in Precoce is 2,49 kg.

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