scholarly journals Pharmacognostic and Analytical Profile of Leaves of Erythroxylum Moonii Hochr. – An Ethno-Medicinal Plant

2021 ◽  
Vol 12 (2) ◽  
pp. 353-359
Author(s):  
Perera P A N G ◽  
Acharya Rabinarayan ◽  
Pandya P ◽  
Harisha C R ◽  
Shukla V J

Erythroxylum moonii Hochr. is a shrub or small tree in family Erythroxylaceae and folklore claims report the use of its leaves in the treatment of helminthiasis. Aim: Hence, present study aims to evaluate the pharmacognostic and analytical characters including HPTLC of its leaves. Methods: E. moonii fresh and powdered leaves were explored for the macroscopic and microscopic features along with their physico-chemical, phytochemical properties and HPTLC following standard procedures. Results: The leaf of E. moonii is simple, alternate, petiolate, stipulate, elliptic and lanceolate with obtusely caudate-acuminate apex, acute base and entire margins. Fresh leaf is light green, slight astringent with characteristic odour and smooth texture. Powdered dry leaves were light green, slight astringent with aromatic odour and coarse, fibrous texture. Microscopic evaluation revealed the presence of simple, bilobed and stellate trichomes (with and without lignification), rosette, rhomboidal, prismatic and cluster crystals, paracytic stomata and starch grains as key characteristics both in fresh leaves and powdered leaves. Obtained values for loss on drying, total ash, acid insoluble ash, pH, water and methanol soluble extractive were 10.44±0.31%, 2.2±0.67%, 0.93±0.06%, 5, 7.63±0.57% and 6.44±0.38% respectively. Qualitative phytochemical analysis suggested the presence of carbohydrates, steroids, glycosides, saponins, alkaloids, tannins and flavonoids in the test sample. HPTLC study revealed 17 and 11 peaks at short (254nm) and long (366nm) ultraviolet consecutively. Conclusion: Obtained results can be used to establish pharmacognostic and analytical standards of leaves of E. moonii which can serve as an important source to determine the quality, purity and strength of the powdered drug. 

Author(s):  
Akhilraj A.R ◽  
Rukmini S ◽  
Amalraj A.R

Background: Citraka or Sveta Citraka (Plumbago zeylanica L.) is an important medicinal plant mentioned in Ayurvedic classics, belongs to the family Plumbaginaceae. The root of the plant exhibits medicinal properties. It has a broad range of pharmacological activities, including anti-inflammatory, antibacterial, antifungal, anticancer, antidiabetic, antioxidant, hepatoprotective, wound healing and cytotoxic. Objective: The aim of the study was to evaluate phyto-pharmacognostical standardization with HPTLC fingerprinting of root of P. zeylanica, one of the most accepted and beneficial medicinal plant in Ayurveda. Materials and Methods: P. zeylanica was collected from Konni, Kerala, India during the month of May 2018 and its root was studied for macroscopic, microscopic, physico-chemical, preliminary phytochemical screening and HPTLC fingerprinting following the standard protocol recommended by The Ayurvedic Pharmacopoeia of India and Quality Standards of Indian Medicinal Plants. Results: The root is long, stout, cylindrical and reddish to dark brown in colour. The transverse section of root showed 5-7 rows of small cubical or rectangular celled cork with light yellow walls, polygonal or slightly tangentially elongated cortex cells containing starch grains and coloured contents, phloem containing small groups of fibres, radially arranged xylem vessels and narrow, 1-4 seriate medullary rays. Physico-chemical analysis showed water soluble extractive value as 26.11±0.01 w/w and alcohol soluble extractive value as 15.85±0.00 w/w. Preliminary phytochemical analysis of root decoction showed the presence of alkaloids, carbohydrates, tannins, saponins, resin and quinones. Conclusion: The information obtained from this research work may be useful to establish the botanical as well as analytical standards for the root of P. zeylanica.


2021 ◽  
Vol 11 (6) ◽  
pp. 2507
Author(s):  
Zina T. Alkanan ◽  
Ammar B. Altemimi ◽  
Asaad R. S. Al-Hilphy ◽  
Dennis G. Watson ◽  
Anubhav Pratap-Singh

Various technologies have been evaluated as alternatives to conventional heating for pasteurization and sterilization of foods. Ohmic heating of food products, achieved by passage of an alternating current through food, has emerged as a potential technology with comparable performance and several advantages. Ohmic heating works faster and consumes less energy compared to conventional heating. Key characteristics of ohmic heating are homogeneity of heating, shorter heating time, low energy consumption, and improved product quality and food safety. Energy consumption of ohmic heating was measured as 4.6–5.3 times lower than traditional heating. Many food processes, including pasteurization, roasting, boiling, cooking, drying, sterilization, peeling, microbiological inhibition, and recovery of polyphenol and antioxidants have employed ohmic heating. Herein, we review the theoretical basis for ohmic treatment of food and the interaction of ohmic technology with food ingredients. Recent work in the last seven years on the effect of ohmic heating on food sensory properties, bioactive compound levels, microbial inactivation, and physico-chemical changes are summarized as a convenient reference for researchers and food scientists and engineers.


Author(s):  
Chandana Majee ◽  
Rupa Mazumder ◽  
Alka N. Choudhary

Background: Trapa natans L., is annual aquatic plant generally kwon as Water caltrp, Water chest nut belonging to the Trapaceae or Lytraceae family. Trapa natans L is use for the treatment of wide no of diseases without proper standardization. Objective: To give the right pharmacognostical and photochemical information of the Trapanatan L leaves. In this study pharmacognostical investigation of the fresh leaves and powder drug were done to determine the macroscopical, microscopical, quantitative physicochemical and phytochemical property of the drugs. Method: Macroscopical, quantitative and qualitative microscopy, physicochemical evaluation, extractive value, florescence analysis and phytochemical analysis were done according to the WHO guideline. Result: Macroscopical analysis showed that, leaves are greenish to purplish color, rhomboidal shape; alternate, acute, margin is dentate, pinnate venation. Microscopic evaluation showed that leaf is dorsi ventral in nature, upper layer epidermis cells were covered with cuticle layer. Single layer of barrel shape cell were present bellow the upper epidermis layer. Trichomes are generally multicellular. Anomocytic stomata were observed in upper epidermis. From the experiment it was found that methanolic extract give the highest extractive value. Phytochemical analysis gives the evidence for the presence of carbohydrate, alkaloids, glycoside, steroids, flavonoids, tannin, and triterpenoids. Qualitative phytochemical analysis give the evidence for presence of high amount total phenolic content. Conclusion: Different pharmacognostical parameters assessed in this examination help to detection and standardization of Trapa natans L., leaves.


Author(s):  
Jigyasa Pathak ◽  
Aswathi M. P. ◽  
B. R. Patel ◽  
Harisha C. R. ◽  
Shukla Vinay J.

Background: Desmodium velutinum (Fabaceae) is one of the wild edible plants used traditionally to treat diarrhoea, haematuria, infertility and also externally used to treat scorpion bites. Though various parts of the plant have been used for therapeutic purpose, panchanga of the plant has not been studied for its Pharmacognostical and phytochemical characteristics in detail, following scientific parameters. Hence in the present study Desmodium velutinum whole plant has been studied for its pharmacognostical and phytochemical characters and compared with commonly used species Desmodium gangeticum. Methods: Whole plant of Desmodim velutinum was collected from Paikmal, Odisha and Desmodium gangeticum was collected from Junagarh, Gujarat in the month of November 2019 following good collection practices and shade dried. Pharmacognostical study and analytical study were performed following standard pharmacopoeial procedures. Results: Whole plant (Panchanga) of former is sweetish and latter is bitter. Powder microscopy of Desmodium gangeticum showed presence of rhomboidal crystals and lignified annular and spiral vessels. Desmodium velutinum showed presence of prismatic crystals and lignified pitted vessels. Physico-chemical analysis showed higher loss on drying in Desmodium gangeticum, and higher water soluble extractive values of Desmodium velutinum. Phytochemical estimation showed the presence of Proteins, tannins, and carbohydrates in both samples. HPTLC study showed similar Rf value 0.82 and 0.94 at 254 and 0.03 and 0.41 at 366nm. Conclusion: Basing upon the analytical and microscopic similarities Desmodium velutinum may be considered as substitute of Desmodium gangeticum


Author(s):  
Attah M.O.O ◽  
Jacks T.W ◽  
Garba S.H. ◽  
Mshelia H.E.

Background:  Leptadenia hastata is a leafy, climbing plant of whose root, leaf and bark is widely consumed in Northern Nigeria. Decoctions made from the leaf is locally used in treatment of diabetes as well as for numerous trado-medicinal processes. This study investigates the physico-chemical as well as phytochemical compounds present in the n-hexane extract of Leptadenia hastata. Method: Physical and chemical profile was determined using Smart Spectro test kits and a mass spectrometer to determine the presence of elements and physical properties. Results: Phytochemical analysis revealed the presence of steroids, triterpenoids and cardiac glycosides. The elemental analysis revealed that Na, SO4 and PO4 had the highest levels in the extract. Pb, Hg and Cd were not detected which showed that the plant extract was not toxic. Other elements (NO4, Mn, Fe, K, Ni, Si, Cn, Zn, Ca, Mg, NH4, Cr and F) were present in varying concentrations which may contribute to its nutritive composition. Conclusions: Various elements present in Leptadenia hastata may have either a direct or indirect role in the control and management of diabetes mellitus, since diabetes is associated with marked alterations in trace elements concentrations and may replace diminished element stores in the treatment of diabetes mellitus.


2014 ◽  
Vol 1 (2) ◽  
pp. 105-108
Author(s):  
Sharmila S ◽  
Kalaichelvi K ◽  
Jayanthi G

Medicinal plants are an integral component of alternative medical care. For millennia, Indian people traditionally played an important role in the management of biological resources and were custodians of related knowledge that they acquired through trial and error over centuries. India has a rich wealth ofmedicinal plants and the potential to accept the challenge to meet the global demand for them. In recent time, focus on plant research has increased all over the world and a large body evidence has collected to show immense potential of medicinal plants used in various traditional system. Plumbago zeylanica L.(Plumbaginaceae) is a tropical plant and the source for the well known ayurvedic drug ‘chitrakah’. Different parts of this plant are widely used for variety of medicinal treatments. Especially used for appetite, indigestion, piles, worms, liver diseases and cancer treatments. The present study was therefore carried outto provide pharmacognostic details of root of Plumbago zeylanica. The physico-chemical analysis of ash content indicated the presence of inorganic matter and siliceous matter in the drug. The phytochemical analysis indicated the presence of alkaloids, phenols, flavonoids, tannins and terpenoids. Plumbagin is anaturally occurring yellow pigment, produced by member of plumbaginaceae that can be obtained from roots


2021 ◽  
Vol 8 (8) ◽  
pp. 440-449
Author(s):  
Fathima Suman P ◽  
Minhaj . ◽  
I Arthi

This study deals with the Pharmacognostical physicochemical and phytochemical evaluation of the whole plant of Piper longum L. Pharmacognostical studies include macroscopic, microscopic and physicochemical evaluation. Physicochemical constants showed the values of total ash (10.8%), acid insoluble ash (1.3%), water soluble ash (3.2%), sulphated ash (12%), nitrated ash (1.85%), hot extraction (11.2%), water soluble extractive (4.8%), ethanol soluble extractive (12.4%), ether soluble extractive (5.6%), loss on drying (29%), swelling index (0.33) and mucilage content (0.8%) w/w. The preliminary phytochemical screening of the alcoholic extract showed presents of alkaloids, phenolic compounds, tannins, mucilage, starch, steroid and flavonoids. This study helps to development of different standard parameters of the plant which help the plant identification and authentication. Keywords: Piper longum L., Whole plant, Pharmacognostic, Phytochemical, Extract.


2021 ◽  
pp. 114-124
Author(s):  
. Shilpa ◽  
Sangita Sood ◽  
Farhan Mohiuddin Bhat

The present research was done to analyse the physico-chemical, Nutritional, Mineral, functional and phytochemical analysis in Functional cereals; oats, pearl millet, sorghum and finger millet. The protein content of the analyzed cereals varied from 7.45% to 14.69% with oats having higher concentration of protein content. The higher fibre content in oats accounted for its highest WAC while as the lowest fibre content in sorghum (2.35%) masked its WAC despite containing the highest carbohydrate content. Neutral detergent fiber that gives the measurement insoluble fibre was found highest in sorghum (11.29%) and lowest in pearl millet (5.56%). Total sugars comprising of reducing and non reducing sugars were found highest in pearl millet (2.88%) followed by sorghum (2.14%) and the least content was found in finger millet (1.69%). Oats were found to possess highest content of phosphorus (381.02mg/100g) and finger millet the lowest (8.21mg/100g). Resistant starch that is inaccessible to enzymes was found highest in oats (2.69g/100g) and lowest in sorghum (1.74g/100g).


Author(s):  
M. I. Uneze ◽  
D. N. Azionu

Various parts of psidium guajava linn are used in ethnomedicine in controls and treatments of different disorders such as liver, heart, and blood related disorders [11]. This research work investigate qualitative phytochemical composition of ethanol leaf extract of psidium guajava linn and its effect on some hematological parameters in albino rats. Qualitative phytochemical analysis of the extract was performed by standard procedures. A total of 20 adult male albino rats were used, they were randomly placed into 5 groups (A, B, C, D and E) each group containing 4 rats groups A, B, C, and D were administered, via oral intubation, the extract at doses of 200, 400, 600, and 800 mg/kg body weight respectively of the extract for 7 consecutive days. Phytochemical screening shows that the extract was rich in tannins anthraquinones, and flavonoid while alkaloids, saponins, terpenes and glycoside were slightly present. There was a decrease in the average body weight, physical activities, and feed and water intake of the rats in the test groups relative to the control hemoglobin concentrate, total white blood cell and packed cell volume recorded in the groups  administered the extract were significantly (p<0.05) higher than in the control this effect on  the haemotological parameters was found to be dose dependent. The findings of this study suggest that the aqueous extract of the fruit plants may be useful in control and treatment of blood related disorders.  These useful applications may be as a result of the phytochemical present in the extract.


Author(s):  
SILPA M ◽  
SURESH JOGHEE ◽  
HAMSALAKSHMI

Objective: Eupatorium glandulosum is a useful medicinal plant belongs to the family Asteraceae. It is traditionally used to treat various diseases such as wound healing, antioxidant, and antiproliferative. The present study was aimed to investigate the physicochemical and phytochemical properties of various extracts of aerial parts of E. glandulosum. Methods: The E. glandulosum plant materials were extracted using the solvents alcohol, ethyl acetate, and chloroform by Soxhlet method. The extracts were screened for physiochemical constants, preliminary phytochemical analysis for carbohydrates, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The quantitative phytochemical analysis was carried out for total flavonoid and total phenols using standard procedures. Results: The physiochemical constituents such as total ash, acid-insoluble ash, and water-soluble ash were found to be 14.25% (w/w), 5% (w/w), and 7.30% (w/w), respectively. The preliminary phytochemical screening confirmed the presence of carbohydrates, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The flavonoid content of the plant extracts was found to be in the descending order ethyl acetate ˃alcohol ˃chloroform and the phenolic content was found to be alcohol ˃ethyl acetate ˃chloroform. Conclusion: The result showed the presence of phytochemical constituents and higher values of phenolic and flavonoid content make the plant useful for the formulation of the different drugs for human uses for treating various diseases.


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