EFEK EKSTRAK ETANOL DAUN SINGKONG (Manihot Utilissima Pohl) SEBAGAI OBAT ALTERNATIF ANTI REMATIK TERHADAP RASA SAKIT PADA MENCIT

2018 ◽  
Vol 3 (2) ◽  
pp. 133
Author(s):  
Setya Enti Rikomah ◽  
Elmitra Elmitra ◽  
Diana Gustina Yunita

Medicinal plants used for generations one of which is crop cassava leaves. Cassava leaves is believed to cure rheumatism, gout, anemia, constipation, and to increase endurance. This study aims to determine the Effects Of Ethanol Leaf Extract Singkong (Manihot Utilissima Pohl) As Drug Alternatif Antirheumatic Of Pain On Mice. This study was an experimental study using 25 mice. These animals were divided into five groups where each group consisted of 5 mice. Cassava leaf extracts as a control test, flamar gel as a positive control, and distilled water as a negative control. Observations were made for 45 minutes using a hot excitatory response in the form of a decrease in a lick or jump after the treatments. The results showed a decline in the response of mice to lick the feet or jump to the heat stimuli were administered after administration of cassava leaf extract. Cassava leaf extract has an effect antirheumatic to pain in mice. Based on the analysis of SPSS best dose in this study is the first dose (0.65mg / 20gr mice BB).

MEDISAINS ◽  
2021 ◽  
Vol 19 (3) ◽  
pp. 72
Author(s):  
Gandi Ari Savitri Widayani ◽  
Muhammad Choiroel Anwar ◽  
Sudirman Sudirman

Background: Gonorrhea cases experiencing antibiotic resistance are increasing due to inadequate treatment or failure to contribute to an increase in antibiotic resistance cases; WHO suggests adding herbal therapy treatment methods is expected to minimize the occurrence of drug resistance as much as possible. Previous studies that have been carried out using other herbal plants have not shown any potential to inhibit the growth of Neisseria gonorrhoeae bacteria, seen from the amount of extract concentration and the dosage and content of the compounds in the contents monitoring the time.Objective: To analyze the difference in the effect of various concentrations of Matoa Leafs Extract (Pometia Pinnata) compared to 500mg Levofloxacin on the increase in the growth inhibition of Neisseria gonorrhoeae.Methods: This is true-experimental research with a post-test-only control group with a randomized design. The sample in this study was the microorganism Neisseria gonorrhoeae obtained through vaginal swabs in 2 females (FSW) positive for Gonorrhea by gram staining and microscopic examination and culture on Chocolate Agar Plant (CAP) media. The culture was then suspended in CAP media. Matoa Leaf extract concentration of 100%, 80%, 60%, 40%, 20%, positive control with Levofloxacin 500 mg and negative control with distilled water with four replications and monitoring in 24 hours, 48 hours, 72 hours, and 96 hours. Data analysis used Kruskal-Wallis, Mann-Whitney, and cohen's test. Result: Levofloxacin 500mg in inhibiting Neisseria Gonorrhoeae bacteria is very effective, and Matoa Leaf extracts 60% and 40% P=0,026 have a strong enough potential to inhibit the growth of Neisseria gonorrhoeae bacteria with the same potential strength. The cohen's test 1.4 effect the levofloxacin 500mg provides a significant influence inhibiting Neisseria Gonorrhoeae.Conclusion: Matoa Leaf extract 60%, and 40% have solid antibacterial potential, although not as strong as Levofloxacin 500mg.


2015 ◽  
Vol 33 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Edson LL Baldin ◽  
Thiago LM Fanela ◽  
Luiz ER Pannuti ◽  
Massuo J Kato ◽  
Renata Takeara ◽  
...  

Bemisia tabaci (Hemiptera: Aleyrodidae) biotype B is one of the most limiting pests of tomato crops in the world. Tomato yield is currently dependent on the use of pesticides, which are problematic to farmers, consumers and the environment. A promising alternative to reduce the harmful effects caused by the indiscriminated use of synthetic insecticides is the use of insecticides of botanical origin. This study aimed to evaluate the effect of 3% (w/v) aqueous extracts from different structures of thirteen botanical species on the behavior of B. tabaci biotype B adults, as well as insecticidal activity of such aqueous extracts on B. tabaci eggs, nymphs, and adults infesting tomato plants. A distilled water solution was used as a negative control, and thiamethoxam insecticide (18 g/100 L of water) as a positive control. Leaf extract of Toona ciliata was observed to have the most efficient inhibitory effect in tests of extracts on whitefly behavior. Furthermore, the use of leaf extract of Toona ciliata led to the most drastic reduction in the number of adults and eggs on tomato leaflets. Leaf extract of Piper aduncum led to the greatest observed ovicidal effect (78.00% of non-hatched nymphs); however it was not effective against nymphs and adults. The leaf extracts of Trichilia pallida, Trichilia casaretti, and Toona ciliata showed the highest control indexes (67.9, 60.3, and 55.1%, respectively). For adults mortality, T. pallida was the most effective (72.8%). Our results indicate that application of extracts of T. pallida, T. ciliata, and T. casaretti are promising strategies to manage B. tabaci biotype B on tomato.


PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 268
Author(s):  
Serly D. S. Toding ◽  
Herny E. I. Simbala ◽  
Deby A. Mpila

ABSTRACT One of the potential medicinal plants as traditional medicine is gardenia plant (Gardenia augusta). Gardenia leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and triterpenoids, which are known to have antibacterial abilities..The aim of this research was to determine the antibacterial activity of gardenia leaf extract on the growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi. The examination on the inhibitory power using the Kirby-Bauer method. The Kirby-Bauer method is referring to the diffusion method using paper disc with five treatments, namely the extract of gardenia leaves with a substance concentration of 20%, 40%, and 60% as well positive control (Ciprofloxacin) and negative control (aquades). Based on the research results obtained, it can be concluded that gardenia leaf extract can inhibit growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi at a concentration of 20%, 40% and 60% and classified as strong and medium category. Keywords  :    Gardenia augusta, antibacterial, of Staphylococcus aureus, Escherichia coli, Salmonella thypiABSTRAK  Salah satu tanaman obat dengan potensi sebagai obat tradisional adalah tanaman kacapiring (Garddenia augusta). Daun kacapiring mengandung senyawa alkaloid, flavonoid, tanin, saponin dan triterpenoid, yang berpotensi sebagai antibakteri sehingga digunakan dalam penelitian ini.Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kacapiring terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Salmonella thypi. Pengujian daya hambat menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas dengan 5 perlakuan yaitu ekstrak daun kacapiring dengan konsentrasi 20%, 40%, dan 60% serta kontrol positif (ciprofloxacin) dan kontrol negatif (akuades). Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak daun kacapiring dapat menghambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella thypi pada konsentrasi 20%, 40% dan 60% dan tergolong dalam kategori kuat dan sedang. Kata Kunci  : Kacapiring, Antibakteri, Staphylococcus aureus, Escherichia coli, Salmonella thypi


2020 ◽  
Vol 2 (1) ◽  
pp. p47
Author(s):  
Nyasha Sakadzo ◽  
Kasirai Makaza ◽  
Liberty Chikata

Pesticidal plants offer valuable and sustainable options for managing Lepidopteran pests with considerable health, environmental and economic benefits in smallholder agro-ecosystem. This study Biopesticidal properties of aqueous crude extracts of tobacco (Nicotiana tabacum L.) against fall armyworm (Spodoptera frugiperda J.E Smith) on maize foliage (Zea mays L.) diets. Bio-efficacy of aqueous crude N. tabacum leaf extracts was evaluated under average room temperature at Great Zimbabwe University, Biology laboratory. The treatments were tobacco leaf extracts at four dosage levels (25 %, 33.33%, 41.67 % and 50% W/V ) and a negative control of untreated maize leaf foliage (distilled water) was used. A positive control of Carbaryl 85% WP was also used at label rates. The experiment was arranged in a Complete Randomized Design (CRD) replicated three times. Five larvae were placed into each of the experimental jars with maize foliage diets drenched into 10ml of distilled water in each treatment extract to keep the maize leaf foliage moist. Mortality for each treatment was recorded at 2 hourly intervals for 20 hours. Results showed that tobacco crude aqueous leaf extracts had Antifeedent activities against FAW larvae. The highest dose of 50% had significantly higher mean FAW larval mortalities (p< 0.05) than lower dosage (25%) and the negative control after 20 hours. However, 50% concentration was not significantly different (p>0.05) from the positive control and that of 33.33% and 41.67% dosages. The bioassay indicated that the 33.33% extract was superior in toxicity to 25% dose and the negative control but similar to higher extract doses though inferior to positive control. However, the mean mortality of 50% extract was not significantly different (p<0.05) from that of the positive control. This study recommends that 50% tobacco aqueous crude leaf extract dose to be used when controlling FAW in maize in the smallholder sector.


2021 ◽  
Vol 6 (2) ◽  
pp. 115-123
Author(s):  
Nunuk Shofiati ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok  tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans. 


2021 ◽  
pp. 2282-2289
Author(s):  
Gina Saptiani ◽  
Slamet Budi Prayitno ◽  
Sari Anggarawati

Background and Aim: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black tiger shrimp (Penaeus monodon). Materials and Methods: A total of 330 shrimps were equally assigned into three extract forms (crude extract, ethyl acetate extract, and n-butanol extract, respectively) in which three levels were provided for each extract. Negative control (without leaf extract) and positive control (with oxytetracycline at 0.05 mg/mL) were used, giving a total of 11 experimental treatments. Results: The results showed that shrimps induced into all form of leaf extracts had significantly higher survival rates, clinical symptoms, and pathological anatomy than those negative control (C–) and positive control (C+). Total hemocyte cells, granulocytes, percentage of phagocytic, and prophenoloxidase activity were similar among leaf extract treatments (p>0.05), but those groups were significantly higher than those of C– and C+ (p<0.05). Conclusion: n-butanol leaf extract at 300 mg/L is suggested to be the most effective treatment since it showed the highest efficacy on the parameters observed. Thus, it is possible to use the leaf extract of A. ilicifolius on-farm as a strategy to enhance bacterial disease resistance and prevent mortality.


2019 ◽  
Vol 1 (2) ◽  
pp. 11-16
Author(s):  
Yanna Rotua Sihombing ◽  
Debi Dinha Sitepu

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno®  32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno®  32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.


Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.


2015 ◽  
Vol 10 (3) ◽  
pp. 562 ◽  
Author(s):  
Fatema Nasrin ◽  
Md. Lukman Hakim

<p class="Abstract">In this study the antidiarrheal activity of ethanolic extracts of the leaves of <em>Mikania cordata</em> and <em>Litsea monopetala</em> was evaluated. Diarrhea was induced in mice by oral administration of castor oil (0.5 mL) 30 min after the administration of the extracts. During a 4 hour study the number of diarrheal feces and percentage inhibition of the extracts (200 and 400 mg/kg body weight) was determined. Loperamide (3 mg/kg body weight) served as standard and belonged to the positive control group. The extracts exhibited potent antidiarrheal activity as well as achieved statistically significant p value (p&lt;0.01 and p&lt;0.05) compared to control group. Among the extracts the highest percentage inhibition of defecation (60%) was recorded for leaf extract (400 mg/kg body weight) of <em>L. monopetala</em>. So, the study corroborates the significant antidiarrheal activity of <em>M. cordata</em> and <em>L. monopetala</em> leaf extracts and raises the demand of further sophisticated investigation.</p><p> </p>


Author(s):  
Nur Fitri

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group


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