scholarly journals Decreased circulating clusterin reflects severe liver complications after hepatoportoenterostomy of biliary atresia

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wanvisa Udomsinprasert ◽  
Yong Poovorawan ◽  
Voranush Chongsrisawat ◽  
Paisarn Vejchapipat ◽  
Sittisak Honsawek
Keyword(s):  
Characteristic Curve ◽  
Linear Regression Analysis ◽  
Diagnostic Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multivariate Linear Regression Analysis ◽  
Severe Liver ◽  
Non Invasive ◽  
Kasai Operation ◽  
Poor Outcomes ◽  
Extracellular Chaperone

AbstractThis study aimed to determine whether circulating levels of clusterin (CLU), an extracellular chaperone implicated in cholestatic and fibrotic processes, are associated with clinical parameters of post-operative BA patients and could serve as a BA biomarker. Ninety-six BA patients and 56 healthy controls were recruited. Circulating CLU levels were measured using enzyme-linked immunosorbent assay. Circulating CLU levels were significantly reduced in BA patients – especially those with worse outcomes including jaundice, severe liver fibrosis, and late-stage of hepatic dysfunction. Multivariate linear regression analysis revealed that circulating CLU levels were negatively associated with outcome parameters indicating jaundice status, degree of fibrosis, and liver dysfunction, but positively correlated with serum albumin and platelet number of BA patients. Lower circulating CLU levels were considerably associated with poor survival of post-operative BA patients. Receiver-operating characteristic curve analysis demonstrated a diagnostic value of circulating CLU as a non-invasive indicator for poor outcomes of BA patients (AUC = 0.85), with a sensitivity of 81.5% and a specificity of 73.5%. All findings indicate that reduced circulating CLU might reflect poor outcomes of BA patients and have potential as a novel biomarker for the disease severity following Kasai-operation.

Three plasma-based microRNAs as potent diagnostic biomarkers for endometrial cancer

2021 ◽  
pp. 1-12
Author(s):  
Xingchen Fan ◽  
Minmin Cao ◽  
Cheng Liu ◽  
Cheng Zhang ◽  
Chunyu Li ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Operating Characteristic ◽  
Characteristic Curve ◽  
External Validation ◽  
Diagnostic Value ◽  
Diagnostic Biomarkers ◽  
Non Invasive ◽  
Polymerase Chain ◽  
Public Datasets ◽  
Plasma Mirnas

BACKGROUND: MicroRNAs (miRNAs), with noticeable stability and unique expression pattern in plasma of patients with various diseases, are powerful non-invasive biomarkers for cancer detection including endometrial cancer (EC). OBJECTIVE: The objective of this study was to identify promising miRNA biomarkers in plasma to assist the clinical screening of EC. METHODS: A total of 93 EC and 79 normal control (NC) plasma samples were analyzed using Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) in this four-stage experiment. The receiver operating characteristic curve (ROC) analysis was conducted to evaluate the diagnostic value. Additionally, the expression features of the identified miRNAs were further explored in tissues and plasma exosomes samples. RESULTS: The expression of miR-142-3p, miR-146a-5p, and miR-151a-5p was significantly overexpressed in the plasma of EC patients compared with NCs. Areas under the ROC curve of the 3-miRNA signature were 0.729, 0.751, and 0.789 for the training, testing, and external validation phases, respectively. The diagnostic performance of the identified signature proved to be stable in the three public datasets and superior to the other miRNA biomarkers in EC diagnosis. Moreover, the expression of miR-151a-5p was significantly elevated in EC plasma exosomes. CONCLUSIONS: A signature consisting of 3 plasma miRNAs was identified and showed potential for the non-invasive diagnosis of EC.

Urine β-2-glycoprotein 1 as a biomarker for diagnosis of systemic lupus erythematosus

Lupus ◽  
2021 ◽  
pp. 096120332110142
Author(s):  
Jung Sun Lee ◽  
Eun-Ju Lee ◽  
Jeonghun Yeom ◽  
Ji Seon Oh ◽  
Seokchan Hong ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Medical Center ◽  
Characteristic Curve ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Systemic Lupus ◽  
Asan Medical

Objective The need for a biomarker with robust sensitivity and specificity in diagnosing systemic lupus erythematosus (SLE) remains unmet. Compared with blood samples, urine samples are more easily collected; thus, we aimed to identify such a biomarker based on urinary proteomics which could distinguish patients with SLE from healthy controls (HCs). Methods Urine samples were collected from 76 SLE patients who visited rheumatology clinic in 2019 at Asan medical center and from 25 HCs. Urine proteins were analyzed using sequential windowed acquisition of all theoretical fragment ion spectra-mass spectrometry, and the candidate marker was confirmed by enzyme-linked immunosorbent assay (ELISA). Receiver operating characteristic curve analysis was used to determine the diagnostic value of the candidate biomarker. Results Of 1157 proteins quantified, 153 were differentially expressed in urine samples from HCs. Among them were previously known markers including α-1-acid glycoprotein 1, α-2-HS-glycoprotein, ceruloplasmin, and prostaglandin-H2 D-isomerase. Moreover, the amount of β-2 glycoprotein (APOH) was increased in the urine of patients with SLE. The ELISA results also showed the level of urine APOH was higher in patients with SLE than in HCs and patients with rheumatoid arthritis. Moreover, the level was not different between SLE patients with and without nephritis. The urine APOH had an area under the curve value of 0.946 at a cut-off value of 228.53 ng/mg (sensitivity 91.5%, specificity 92.0%) for the diagnosis of SLE. Conclusion The results indicate that the urine APOH level can be an appropriate screening tool in a clinical setting when SLE is suspected.

scholarly journals Immunoglobulin G Antibody against Helicobacter pylori: Clinical Implications of Levels Found in Serum

2002 ◽  
Vol 9 (5) ◽  
pp. 1044-1048 ◽  
Author(s):  
Tseng-Shing Chen ◽  
Fen-Yau Li ◽  
Full-Young Chang ◽  
Shou-Dong Lee
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Predictive Value ◽  
Linear Regression Analysis ◽  
Serum Antibody ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multivariate Linear Regression Analysis ◽  
H Pylori ◽  
Antibody Levels

ABSTRACT The clinical significance of high levels of antibody against Helicobacter pylori is still unclear. We sought to evaluate whether the serum antibody levels could predict the presence of macroscopic gastroduodenal disease, to identify factors that correlate with antibody levels in a multivariate context, and to determine the predictive value of antibody levels for diagnosing H. pylori infection. The grades of gastritis and density of H. pylori colonization were scored separately using the updated Sydney system for antral and body mucosa. An enzyme-linked immunosorbent assay (ELISA) for the quantitative detection in serum of IgG antibodies to H. pylori was performed. Of the 170 dyspeptic patients, 105 (62%) had H. pylori infection. There was no difference in antibody levels among endoscopic findings of normal mucosa, chronic gastritis, and duodenal ulcer. On multivariate linear regression analysis, the status of H. pylori infection, mononuclear cell infiltration of body mucosa, and age correlated with antibody levels. The negative predictive value for antibody levels of <30 U/ml is 94%, and the positive predictive value of antibody levels of >70 U/ml is 98%. We conclude that serum antibody levels do not predict the severity of gastroduodenal diseases or the density of H. pylori colonization in H. pylori-infected dyspeptic patients. Higher levels are associated with the presence of H. pylori infection, the chronic gastritis score of the corpus, and older age. Setting a gray zone is necessary for ELISA, since the accuracy in this zone does not allow a precise determination of H. pylori status.

scholarly journals Relationship Between Arterial Stiffness and Cardiovascular Risk Scores Predicted by 2 Different Models

2021 ◽  
Vol 34 (2) ◽  
pp. 226-227
Author(s):  
Ting-jun Wang ◽  
Xiao-qi Cai ◽  
Ling-yu Zhang ◽  
Yi-hua Shen ◽  
Qun-fang Xie ◽  
...  
Keyword(s):  
Waist Circumference ◽  
Arterial Stiffness ◽  
Characteristic Curve ◽  
Linear Regression Analysis ◽  
Risk Scores ◽  
Atherosclerotic Cardiovascular Disease ◽  
Multivariate Linear Regression Analysis ◽  
Cross Sectional ◽  
Receiver Operation Characteristic ◽  
Ascvd Risk

Abstract Background To investigate the relationship between arterial stiffness and 10-year atherosclerotic cardiovascular disease (ASCVD) risk predicted by models of pooled cohort equations (PCEs) or ASCVD risk in China (China-PAR). Methods A cross-sectional clinical study was carried out, in which clinical data were collected from 1,090 subjects aged 30–75 years who were admitted to a university affiliated hospital from 2016 to 2018. Arterial stiffness was examined by carotid–femoral pulse wave velocity (cfPWV). All subjects were divided into 2 groups: normal arterial stiffness (cfPWV &lt;10 m/second) and elevated arterial stiffness (cfPWV ≥10 m/second). Ten-year ASCVD risk was predicted by either PCE or China-PAR. Results ASCVD risks predicted by 2 models in the elevated arterial stiffness group were higher than those in the normal arterial stiffness group [PCE: 16.5% (8.6%–28.3%) vs. 6.3% (2.7%–12.3%); China-PAR: 8.8% (6.1%–12.4%) vs. 3.9% (2.1%–6.6%), both P &lt; 0.001]. The correlation coefficient between cfPWV and ASCVD risk predicted by China-PAR was greater than that by PCE (0.573 vs. 0.503, z = 5.272, P &lt; 0.001). Multivariate linear regression analysis showed that 10-year ASCVD risk predicted by PCE model (β = 0.475, P &lt; 0.001) and waist circumference (β = 0.092, P = 0.001) correlated with cfPWV. However, when PCE was replaced by China-PAR, only ASCVD risk (β = 0.573, P &lt; 0.001), not waist circumference, was associated with arterial stiffness. Receiver operation characteristic curve showed that the discrimination of 10-year ASCVD risk predicted by China-PAR for arterial stiffness was better than PCE (area under curve 0.814 vs. 0.767, z = 4.992, P &lt; 0.001). A stratification analysis revealed that the better discrimination by China-PAR mainly came from males. Conclusions Ten-year ASCVD risks predicted by either China-PAR or PCE are associated with arterial stiffness. The association is stronger when the risk is predicted by China-PAR, especially in males.

scholarly journals Serum SARS-COV-2 Nucleocapsid Protein: A Sensitivity and Specificity Early Diagnostic Marker for SARS-COV-2 Infection

2020 ◽  
Author(s):  
Tao Li ◽  
Li Wang ◽  
Huihui Wang ◽  
Xuemei Li ◽  
Shubing Zhang ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Sensitivity And Specificity ◽  
Early Stage ◽  
Characteristic Curve ◽  
Diagnostic Value ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cutoff Value ◽  
Protein Assay ◽  
N Protein ◽  
Positive Rate

AbstractObjectiveThis study aimed to explore the diagnostic value of serum severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid(N) protein assay in the early stage of SARS-COV-2 infection.MethodSerum N protein in SARS-COV-2 infected patients and non-SARS-COV-2 infected population was measured by enzyme-linked immunosorbent assay (ELISA) double antibody sandwich assay. Colloidal gold immunochromatography assay is used to detect serum N protein antibodies in the above population.Results50 cases of SARS-CoV-2 nucleic acid positive and SARS-CoV-2 antibody negative patients had a serum N protein positive rate of 76%, including 2% with a concentration of 10.00-49.99 pg / mL, 8% with a concentration of 50.00-99.99 pg / mL, 22% with a concentration of 100.00 - 299.99 pg/mL, and 44% with a concentration≥ 300.00 pg / mL. 37 samples of patients with serum SARS-CoV-2 antibody positive after infection had a serum SARS-CoV-2 N protein positive rate of 2.7%, of which 2.7% had the concentration of 10.00-49.99 pg / mL and 0% had the concentration of 50.00-99.99 pg / mL, 100.00 −299.99 pg / mL, and >300.00 pg / mL. Serum N protein test results of 633 non-SARS-COV-2 infected patients including pregnant women, other respiratory infections, and increased rheumatoid factor were all negative, having a serum N protein concentration less than 10.00 pg/mL, with a specificity of 100%. Using SPSS 19.0 to calculate the receiver operating characteristic curve, the area under the curve was 0.9756 (95% confidence interval 0.9485-1.000, p <0.0001), sensitivity and specificity were 92% (95% confidence interval 81.16% to 96.85%) and 96.84% (95% confidence interval 95.17% to 97.15%). The best CUTOFF value is 1.850 pg / mL.ConclusionThe measurement of SARS-COV-2 serum N protein has a high diagnostic value for the infected patients before the antibody appears, and shortens the window period of serological diagnosis. The laboratory needs to establish an individual CUTOFF value according to purpose of the application.

MicroRNA-96 is downregulated in sepsis neonates and attenuates LPSinduced inflammatory response by inhibiting IL-16 in monocytes

2020 ◽  
Vol 23 ◽  
Author(s):  
Chunlei Zhang ◽  
Xiuting Li ◽  
Na Liu ◽  
Zijian Feng ◽  
Chengyuan Zhang
Keyword(s):  
Inflammatory Response ◽  
Inflammatory Responses ◽  
Diagnostic Value ◽  
Luciferase Reporter ◽  
Enzyme Linked Immunosorbent Assay ◽  
Real Time Quantitative Pcr ◽  
Non Invasive ◽  
Causes Of Mortality ◽  
Underlying Mechanisms ◽  
Relationship Of

Background: Neonatal sepsis (NS) remains one of the leading causes of mortality among newborns. This study found the deregulated microRNA-96 (miR-96) in NS neonates, and aimed to evaluate the clinical significance of miR-96, as well as its effect on LPS-induced inflammatory response in monocytes. In addition, the relationship of interleukin-16 (IL16) and miR-96 was investigated to understand the underlying mechanisms. Methods: Expression of miR-96 was examined using real-time quantitative PCR. Monocytes stimulated by LPS was used to mimic excessive inflammation in the pathogenesis of NS. The enzyme-linked immunosorbent assay was applied to evaluate pro-inflammatory cytokines levels. A luciferase reporter assay was used to confirm the interaction between miR-96 and IL16. Results: Serum miR-96 expression was decreased in NS newborns and had considerable diagnostic value for NS screening. LPS inhibited miR-96 expression in monocytes, and the overexpression of miR-96 could reverse the effects of LPS on the inflammation of monocytes. IL-16 was a target gene of miR-96 and negatively correlated with miR-96 levels in NS neonates. The inhibited inflammatory responses induced by miR-96 overexpression was abolished by the elevated IL-16 in monocytes. Conclusion: All the data reveal that serum decreased miR-96 may serve as a candidate non-invasive biomarker for NS diagnosis. In addition, miR-96 inhibits LPS-induced inflammatory responses by targeting IL-16 in monocytes. The miR96/IL-16 axis may provide novel therapeutic targets for NS treatment.

scholarly journals Relationship Between Arterial Stiffness and Cardiovascular Risk Scores Predicted by 2 Different Models

2021 ◽  
Vol 34 (3) ◽  
pp. 299-300
Author(s):  
Ting-jun Wang ◽  
Xiao-qi Cai ◽  
Ling-yu Zhang ◽  
Yi-hua Shen ◽  
Qun-fang Xie ◽  
...  
Keyword(s):  
Waist Circumference ◽  
Arterial Stiffness ◽  
Characteristic Curve ◽  
Linear Regression Analysis ◽  
Risk Scores ◽  
Atherosclerotic Cardiovascular Disease ◽  
Multivariate Linear Regression Analysis ◽  
Cross Sectional ◽  
Receiver Operation Characteristic ◽  
Ascvd Risk

Abstract Background To investigate the relationship between arterial stiffness and 10-year atherosclerotic cardiovascular disease (ASCVD) risk predicted by models of pooled cohort equations (PCEs) or ASCVD risk in China (China-PAR). Methods A cross-sectional clinical study was carried out, in which clinical data were collected from 1,090 subjects aged 30–75 years who were admitted to a university affiliated hospital from 2016 to 2018. Arterial stiffness was examined by carotid–femoral pulse wave velocity (cfPWV). All subjects were divided into 2 groups: normal arterial stiffness (cfPWV &lt;10 m/second) and elevated arterial stiffness (cfPWV ≥10 m/second). Ten-year ASCVD risk was predicted by either PCE or China-PAR. Results ASCVD risks predicted by 2 models in the elevated arterial stiffness group were higher than those in the normal arterial stiffness group [PCE: 16.5% (8.6%–28.3%) vs. 6.3% (2.7%–12.3%); China-PAR: 8.8% (6.1%–12.4%) vs. 3.9% (2.1%–6.6%), both P &lt; 0.001]. The correlation coefficient between cfPWV and ASCVD risk predicted by China-PAR was greater than that by PCE (0.573 vs. 0.503, z = 5.272, P &lt; 0.001). Multivariate linear regression analysis showed that 10-year ASCVD risk predicted by PCE model (β = 0.475, P &lt; 0.001) and waist circumference (β = 0.092, P = 0.001) correlated with cfPWV. However, when PCE was replaced by China-PAR, only ASCVD risk (β = 0.573, P &lt; 0.001), not waist circumference, was associated with arterial stiffness. Receiver operation characteristic curve showed that the discrimination of 10-year ASCVD risk predicted by China-PAR for arterial stiffness was better than PCE (area under curve 0.814 vs. 0.767, z = 4.992, P &lt; 0.001). A stratification analysis revealed that the better discrimination by China-PAR mainly came from males. Conclusions Ten-year ASCVD risks predicted by either China-PAR or PCE are associated with arterial stiffness. The association is stronger when the risk is predicted by China-PAR, especially in males.

scholarly journals CA72-4 may contribute to real-time reconnaissance of gastric cancer

F1000Research ◽  
2012 ◽  
Vol 1 ◽  
pp. 33 ◽  
Author(s):  
Jutta Keller ◽  
Ella Reiss-Sklan ◽  
Miri Refael ◽  
Viola Andresen ◽  
Yael Levy-Herman ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Screening ◽  
Gastric Carcinoma ◽  
Positive Impact ◽  
Linear Regression Analysis ◽  
Gastric Fluid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multivariate Linear Regression Analysis ◽  
Screening Programs ◽  
Gastric Cancer Screening

Objective: Data from prospective studies indicate a positive impact of gastric cancer screening programs on mortality associated with the disease. Unfortunately, endoscopic procedures, widely regarded as uncomfortable, face low patient compliance, thus underscoring the need for reliable biological markers capable of detection of tumor growth in bodily fluids. Furthermore, in light of the emerging patient-friendly, still devoid of histopathological capabilities, capsule endoscopy, gastric fluid may prove valuable for biomarker-assisted cancer diagnosis. We set out to determine whether CA72-4 measurement in gastric fluid may be of benefit for detection of gastric cancer.Design: Open prospective study.Setting: Sample collection was performed at a tertiary referral center for patients with gastroenterological diseases; immunological analysis was performed at the R&D facility of a commercial biotechnology company. Studies were part of an EU-FP6 project (NEMO).Patients: 176 patients referred for endoscopy due to gastrointestinal complaints.Interventions: Gastric juice was aspirated endoscopically according to standard operating procedures, volume and pH were measured immediately and samples stored at -80°C.Outcome measures: Concentration of CA72-4 tumor marker was evaluated by enzyme-linked immunosorbent assay (ELISA).Results: Median CA72-4 levels were about 4-fold higher in cancer patients compared with patients with normal gastric findings, gastric inflammation, intestinal metaplasia or other diseases (p=0.001). Multivariate linear regression analysis revealed that elevated CA72-4 was significantly predicted by gastric carcinoma adjusted for H. pylori status, age, smoking status, PPI dose, and pH of aspirate (R2=0.27, p<0.0001). In this model, diagnosis of gastric carcinoma had by far the greatest influence. At a cut-off level of 100 U/ml, CA72-4 had 75% sensitivity and 89% specificity for detection of gastric cancer.Conclusions: Based on our findings, CA72-4 level assessment in gastric fluid, featuring yet unmatched accuracy of malignant neoplasia detection may prove beneficial for gastric cancer screening.

scholarly journals AHSG Thr256Ser Gene Polymorphism as a Predictor of Low Serum Fetuin-A Levels in Indonesian Maintenance Hemodialysis Patients

2020 ◽  
Vol 8 (A) ◽  
pp. 185-190
Author(s):  
Riri Andri Muzasti ◽  
Suhardjono Suhardjono ◽  
M. B. Purwanto ◽  
R. J. Sembiring
Keyword(s):  
Gene Polymorphism ◽  
Linear Regression Analysis ◽  
Assay Method ◽  
Maintenance Hemodialysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Multivariate Linear Regression Analysis ◽  
Cross Sectional ◽  
Fetuin A ◽  
Cardiovascular Morbidity And Mortality ◽  
Low Serum

BACKGROUND: Vascular calcification (VC) is a risk factor for cardiovascular morbidity and mortality in maintenance hemodialysis (MHD) patients. The effect of alpha2-Heremans Schmid glycoprotein (AHSG) Thr256Ser gene polymorphism toward serum fetuin-A levels as one of the VC inhibitors in MHD patients is still unclear. OBJECTIVE: This study aims to investigate the relationship between AHSG Thr256Ser gene polymorphism toward serum fetuin-A levels in MHD patients in Indonesia. METHODS: The research study design used was cross-sectional. Serum fetuin-A levels were assessed by enzyme-linked immunosorbent assay method, and polymerase chain reaction-restriction fragment length polymorphism determined AHSG Thr256Ser gene polymorphism. Multivariate linear regression analysis was used to analyze the factors related to serum fetuin-A levels. p < 0.05 was considered as statistically significant. RESULTS: Median value of serum fetuin-A levels was 235.0 pg/ml (ranging 78–756) with mean 259.99 ± 119.36 pg/ml. Out of 106 patients involved, the distribution of AHSG Thr256Ser genotype frequency was 54 (50.9%) respondents had CG genotype, 46.2% had CC genotype, and the others (2.8%) had GG genotype. Patients with homozygous C allele (CC genotype) were 0.1 times more protected (CI 95%: 0.1–0.3) to have low serum fetuin-A levels, compared with patients with G allele either in a homozygote (GG genotype) or in the heterozygote (CG genotype). From the multivariate analysis results, it can be obtained a formulation in predicting serum fetuin-A level, which is: Serum fetuin-A level prediction = 276,59 + 150,49 DM - 0,26 serum interleukin -6 level - 43,58 AHSG Thr256Ser gene polymorphism CONCLUSIONS: AHSG Thr256Ser gene polymorphism had a significant relationship with serum fetuin-A levels in MHD patients in Indonesia. Subjects with G allele (CG and GG genotypes) had lower serum fetuin-A levels than those with CC genotype.

Diagnostic and Therapeutic Value of Procalcitonin and D-dimer in Sepsis Study on the Expression of Procalcitonin (PCT) and D-dimer (D-D) in Serum of Sepsis Patients and Their Correlation

2021 ◽  
Vol 7 (4) ◽  
pp. 557-567
Author(s):  
Jingyuan Zhao ◽  
Long Dong ◽  
Xue Wu ◽  
Xiaowei Yan ◽  
Chong Liu ◽  
...  
Keyword(s):  
Characteristic Curve ◽  
Diagnostic Value ◽  
Inflammatory Factors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Positive Correlation ◽  
Increased Risk ◽  
Tnf Α ◽  
Experimental Group ◽  
D Dimer

Altogether 89 septic patients admitted to our hospital from February 2018 to March 2019 were selected as the experimental group, and 60 healthy people underwent physical examination during the same period were selected as the control group. The expression of PCT and D-D in serum was detected by enzyme-linked immunosorbent assay (ELISA), and the diagnostic value of PCT and D-D in septic patients was evaluated by receiver operating characteristic curve (ROC). Peason correlation coefficient was used to analyze the correlation between PCT and D-D, and the correlation among inflammatory factors interleukin-6 (IL-6), platelet activating factor (PAF), tumor necrosis factor -α (TNF-α). Logistic regression was used to analyze the independent risk factors affecting the onset of sepsis. The expressions of serum PCT and D-D in septic patients were higher than those in the control group. AUC of patients with sepsis diagnosed by serum PCT and D-D were 0.929 and 0.905. The expression of IL-6, PAF and TNF-α in the experimental group was significantly higher than that in the control group. There was a positive correlation between PCT and D-D in the experimental group, and a positive correlation of PCT, D-D with IL-6, PAF and TNF-α. Patients with old age, high lactic acid, high CRP, high urea nitrogen, high serum creatinine, high PCT and high D-D expression have an increased risk of sepsis. Our study showed that the expression of PCT and D-D increases in septic patients, which has a satisfactory diagnostic value for septic patients, and inhibition of PCT and D-D expression can be used as a potential therapeutic target for septic patients.

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