scholarly journals Hyperbaric Oxygen (HBO) Reduce Expression of Hypoxia Inducible Factor-1 Alpha (HIF-1 alpha) and Endometriotic Tissue Size in Mice Model of Endometriosis

2020 ◽  
Vol 151 ◽  
pp. 01003
Author(s):  
Dedy Syahrizal ◽  
Cut Mustika ◽  
Teuku Renaldi ◽  
Mohammad G. Suryokusumo ◽  
Hendy Hendarto
Keyword(s):  
Hyperbaric Oxygen ◽  
Hypoxia Inducible Factor ◽  
Control Group ◽  
Mice Model ◽  
Control Group Design ◽  
Hypoxia Inducible Factor 1 ◽  
Peritoneal Tissue ◽  
Implant Size ◽  
Experimental Laboratory ◽  
Tissue Size

Hypoxia in endometriosis will increase the expression of Hypoxia Inducible Factor-1alpha (HIF1alpha) and its expression could be decreased by Hyperbaric Oxygen (HBO). This study aimed to analyze the effect of HBO 2.4 ATA for 3x30 minutes per day for 10 days on the expression of HIF-1 alpha and endometriotic tissue size on mice model of endometriosis. This study was an experimental laboratory study with a separate pretest-posttest control group design. The mice were divided into three groups, the first was a pretest control group (which describes the condition after endometrium transplantation), the second was the endometriotic group that received hyperbaric oxygen, and the third was the endometriotic group that did not receive hyperbaric oxygen therapy. The endometriosis implant size in the peritoneal tissue was assessed and the immunohistochemistry examination was conducted to determine the expression of HIF-1 alpha. The endometriosis tissue size was reduced in the HBO group compared to the control and nonHBO group. The lowest expression of HIF-1 alpha was significantly found in HBO over the other group. The decrease of HIF1 alpha expression mediates the reduction of size endometriotic tissue due to the therapy of HBO.

scholarly journals Hypoxia Inducible Factor-1-Alpha Expression on Preeclampsia Mice Model With L-Arginine Administration

2021 ◽  
Vol 57 (3) ◽  
pp. 226
Author(s):  
Nutria Widya Purna Anggraini ◽  
Sri Sulistyowati ◽  
Muhammad Adrianes Bachnas ◽  
Eric Edwin Yuliantara ◽  
Wisnu Prabowo ◽  
...  
Keyword(s):  
Treatment Group ◽  
Statistical Tests ◽  
Hypoxia Inducible Factor ◽  
Data Distribution ◽  
Hypoxic Condition ◽  
Control Group ◽  
Mice Model ◽  
Hypoxia Inducible Factor 1 ◽  
Minimum Value ◽  
Maximum Value

Preeclampsia is hypertension in pregnancy that affects 2% to 8% of pregnancies worldwide and causes significant maternal and perinatal morbidity and mortality. In the pathogenesis of preeclampsia, placental hypoxia plays an important role, associated with excessive trophoblast apoptosis resulting in decreased trophoblast and spiral arteries invasion. This placental hypoxic condition will induce increased expression of Hypoxia Inducible Factor -1-Alpha (HIF-1-A). L-Arginine is a potent vasodilator presumably to improve preeclampsia placental hypoxic conditions and reduce HIF-1-A expression. This study was an experimental study with a parallel-group post-test only design. Thirty-six preeclamptic mice models were divided into 2 groups. The control group (K1) 18 preeclamptic mice model without treatment and the treatment group (K2) 18 preeclamptic mice given L-Arginine. The independent variable was the administration of L-Arginine and the dependent variable is the placental HIF-1-A expression. Statistical analysis used unpaired t-test on normal data distribution, and Mann Whitney test on abnormal data distribution. The mean of placental HIF-1-A expression K1 was 2.47 ± 1.65 with a minimum value of 0.4 and a maximum value of 6.6. At K2 0.93 ± 0.55 with a minimum value of 0.0 and a maximum value of 2.0. Statistical tests showed that the placental HIF-1-A expression in the treatment group was significantly lower than that in the control group (p <0.001). In conclusion, the expression of HIF-1-A in preeclamptic mice model placenta decreased with L-Arginine administration.

scholarly journals Uji Sensitisasi Dermal Masker Gel Kombinasi Ekstrak Ampas Daun Teh dan Air Cucian Beras

2019 ◽  
pp. 57-64
Author(s):  
Andi Nurul Annisa ◽  
Wahyu Utaminingrum ◽  
Erza Genatrika
Keyword(s):  
Oryza Sativa L ◽  
Sample Selection ◽  
Challenge Test ◽  
Simple Random Sampling ◽  
Control Group ◽  
Control Group Design ◽  
Experimental Laboratory ◽  
Delayed Hypersensitivity Reaction ◽  
Tea Leaf ◽  
Washing Water

The combination of tea leaf dregs extract and rice washing water as a peel-off gel mask was found to be stable up to 28 days of storage and had an IC50 value 20.4 ppm. This potential can provide an optimal effects as cosmetic ingredients.However, to meet the regulatory requirements of Badan Pengawas Obat Makanan (BPOM) RI and ISO 10993, a dermal sensitization test also need to be done. The aim of this study was to determine the dermal sensitization reaction from peel-off gel mask containing a combination of tea leaf dregsextract (Camellia sinensis L.) and rice washing water (Oryza sativa L.) in test animal. Thisstudy was designed based on experimental laboratory methods and sample selection by simple random sampling. The intervention was given based on posttest-only control group design. The test results for dermal sensitization were determined using the Guinea Pig Maximization Test (GPMT) method. The results showed erythema and edema reactions according to the Magnusson and Kligman scale after administration of peel-off gel masks as samples in the topical tests in test animals, appeared on the 11th day and in the challenge test appeared on the 24th day. This was due to incubation period for one week where the hypersensitivity state can develop, so that this reaction is also called the delayed hypersensitivity reaction

scholarly journals P068 Hyperbaric oxygen reduces hypoxia-inducible factor-1 alpha expression in experimental acute distal colitis

2014 ◽  
Vol 8 ◽  
pp. S91
Author(s):  
R.S. Parra ◽  
E. Uchoa Carreira ◽  
A.H.P. Lopes ◽  
T.M. Cunha ◽  
S.B. Garcia ◽  
...  
Keyword(s):  
Hyperbaric Oxygen ◽  
Hypoxia Inducible Factor ◽  
Hypoxia Inducible Factor 1

scholarly journals JUMLAH ION KROMIUM (Cr) DAN NIKEL (Ni) KAWAT ORTODONTIK STAINLESS STEEL YANG TERLEPAS DALAM PERENDAMAN SALIVA

e-GIGI ◽  
2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Ciendy O. Jura ◽  
Lydia E. N. Tendean ◽  
P. S. Anindita
Keyword(s):  
Stainless Steel ◽  
Oral Cavity ◽  
Artificial Saliva ◽  
Steel Wire ◽  
Stainless Steel Wire ◽  
Control Group ◽  
Ion Release ◽  
Control Group Design ◽  
Orthodontic Wire ◽  
Experimental Laboratory

Abstract: Orthodontic stainless steel wire is one of the instrument components oftenly used in orthodontic treatment and may persist in the oral cavity for a long period of time. Orthodontic stainless steelwire in the oral cavity can be corrosive in the presence of Cr and Ni ion release which can be harmful to the human body and the stainless steel wire itself. This study aimed to determine the amount of Cr and Ni ions released from the stainless steel orthodontic wire that was immersed in artificial saliva. This was an experimental laboratory study with a posttest only control group design. Samples were analyzed by using a UV-Vis spectrophotometry to determine the released Cr and Ni ions in the saliva. Samples consisted of 4 brands of orthodontic stainless steel wires immersed in artificial saliva for 30 days with a temperature of 370C. Data were analyzed by using a computer program. The results showed that the release of Cr ions in samples A, B, C, and D respectively were: 0.302 ppm, 0.331ppm, 0,311 ppm, and 0.483 ppm meanwhile of Ni ions were 1.930 ppm, 1.778 ppm, 1.654 ppm, and 1.391ppm. Conclusion: The release of Cr and Ni ions varied in each sample of orthodontic stainless steel wire .Keywords: orthodontic stainless steel, Cr, Ni, artificial saliva, UV-Vis spectrophotometryAbstrak: Kawat ortodontik stainless steel merupakan salah satu komponen alat yang sering digunakan dalam perawatan ortodontik dan dapat bertahan dalam rongga mulut untuk jangka waktu yang lama. Kawat ortodontik stainless steel yang berada di dalam rongga mulut dapat mengalami korosi dengan adanya pelepasan ion Cr dan Ni yang bersifat merugikan bagi tubuh manusia dan kawat ortodontik itu sendiri. Penelitian ini bertujuan untuk mengetahui besarnya jumlah pelepasan ion Cr dan Ni dari kawat ortodontik stainless steel yang direndam dalam saliva buatan. Penelitian ini menggunakan metode eksperimental laboratorium dengan rancangan posttest only control group. Sampel diuji dengan menggunakan spektrofotometri UV-Vis untuk mengetahui pelepasan ion Cr dan Ni dalam saliva. Sampel terdiri dari 4 merek kawat ortodontik stainless steel direndam dalam saliva buatan selama 30 hari dengan suhu 370C. Data hasil penelitian dianalisis menggunakan program komputer. Hasil penelitian menunjukkan pelepasan ion Cr pada sampel A, B, C, dan D berturut-turut 0,302 ppm; 0,331 ppm; 0,311 ppm; dan 0,483 ppm sedangkan pelepasan ion Ni 1,930 ppm; 1,778 ppm; 1,654 ppm; dan 1,391 ppm. Simpulan: Pelepasan ion Cr dan Ni bervariasi dari masing-masing sampel kawat ortodontik stainless steel.Kata kunci : Kawat ortodontik stainless steel, Cr, Ni, saliva buatan, spektrofotometri UV-Vis

scholarly journals Ethanol Extract of Black Cumin Seed (Nigella Sativa) Reduces Expression Kidney iNOS in Mice Model Preeclampsia

2019 ◽  
Vol 5 (2) ◽  
pp. 88
Author(s):  
Rubiati Hipni
Keyword(s):  
Nigella Sativa ◽  
Ethanol Extract ◽  
Negative Control ◽  
Inos Expression ◽  
Control Group ◽  
Mice Model ◽  
Control Group Design ◽  
Black Cumin ◽  
Black Cumin Seed ◽  
Cumin Seed

This study aims to analyze the effect of ethanol extract of black cumin seeds (Nigella Sativa) on iNOS expression in the kidney of mice model preeclampsia. Design This study uses an experimental design with a posttest only control group design. Using a mice model preeclampsia. This study was divided into 6 groups: negative control, positive control (preeclampsia model), model + black cumin seed extract 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day days, after surgery on mice then examined iNOS Kidney expression by methods Immunohistochemical. Data from observations were analyzed by one way ANOVA test. The result there is an effect of ethanol extract of black cumin seeds on renal iNOS expression in mice model preeclampsia (p <0.05) at doses of 500mg, 1000mg, 1500mg, and 2000mg on decreasing renal iNOS expression. The conclusion Ethanol extract of black cumin seeds (Nigella sativa) can reduce iNOS Kidney expression in Mice Preeclampsia.

scholarly journals Perbedaan daya antiglukan NaOCl 2,5% dan ekstrak kulit manggis (Garcinia mangostana L.) 0,09% terhadap Enterococcus faecalis (Comparison of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract (Garcinia mangostana Linn) 0.09% against Enterococcus faecalis)

2019 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Nabiela Rahardia ◽  
M Rulianto ◽  
Dian Agustin Wahjuningrum
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Bacterial Biofilm ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Control Group Design ◽  
Root Canals ◽  
Viable Cells ◽  
Experimental Laboratory ◽  
The Difference

Background. Failure of endodontic treatment is commonly caused by the persistent microorganisms remaining in the root canal such as Enterococcus faecalis. Enterococcus faecalis can form a biofilm in tough environmental conditions within the root canals and caused biofilm-mediated infections which needs more complicated treatment due to the increasing of antimicrobial resistance. The biofilm formation initial and most important step is bacteria adherence to the solid surface that is mediated by glucan. NaOCl 2.5% is a commonly used root canal medicaments but can cause injury of periapical tissue. Mangosteen pericarp extract contains flavonoid, tannin, and xanthone have mechanism for inhibiting adherence of bacterial biofilm. Difference of antibacterial activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% can be determined by experimental laboratory to determine the adherence of bacteria in each treatment. Purpose. The aim of this study was to assess the difference of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% on Enterococcus faecalis. Method. This study was designed as an experimental laboratory study with post  test only control group design using Enterococcus faecalis ATCC 29212. Mangosteen pericarp was extracted using maceration method. Adherence analysis was observed after 24 hours by examining the viable cells in suspension. These viable cells are measured by UV-Vis spectrophotometer to compare the suspensions’ turbidity. Using the Independent T-Test, significantly less bacteria were found adhering to the mangosteen pericarp extract. Results. Absorbancy difference level by mangosteen pericarp extract 0.09% is significantly greater than the NaOCl 2.5% (p<0.05). Conclusion. Antiglucan activity that generated by mangosteen pericarp extract 0.09% is greater than NaOCl 2.5%

scholarly journals Gambaran Histopatologi Pankreas Tikus Putih (Rattus norvegicus) Pasca Perlakuan Iskemia-Reperfusi Ginjal

2019 ◽  
Vol 13 (1) ◽  
pp. 58
Author(s):  
Elva Rosiana ◽  
Wiwit Ade Fidiawati ◽  
Darmawi Darmawi
Keyword(s):  
Tissue Damage ◽  
Control Treatment ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
White Rats ◽  
Post Test ◽  
Experimental Laboratory ◽  
Histopathologic Feature

Ischemic-reperfusion injury is defined by a condition of hypoperfusion in the spesific organ followed by reperfusion(reoxygenation) inducing tissue damage. Tissue damage produces reactive oxygen species (ROS) leading to oxidativestress condition. Oxidative stress mediate the lipid peroxidation reactions, harm the cell and finally facilitate the celldeath. The aim of this study was to evaluate the histopathologic feature of white rat’s pancreas post renal ischemiareperfusion.This study was an experimental laboratory research with post-test-only control group design including 20male white rats. Rats were divided into 5 groups, control, treatment 1 (45 minutes ischemia), treatment 2 (45 minutesischemia followed by 1 hour reperfusion), treatment 3 (45 minutes ischemia followed by 2 hours reperfusion) andtreatment 4 (45 minutes ischemia followed by24 hours reperfusion). We found the change of the histopathologicalcharacteristics of white rat’s pancreas in term of edema, leukocyte infiltration and vacuolization.

scholarly journals JUS SEMANGKA MENURUNKAN NEUTROFIL TIKUS JANTAN GALUR WISTAR YANG TERPAPAR ASAP ROKOK

2018 ◽  
Vol 11 (2) ◽  
pp. 166
Author(s):  
Rizky Prihandari ◽  
Lailatul Muniroh
Keyword(s):  
Cigarette Smoke ◽  
Hazardous Substances ◽  
Male Rats ◽  
Laboratory Research ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Post Test ◽  
Experimental Laboratory

Cigarette smoke contains many hazardous substances and free radical which affect respiratory system. Watermelon “Sugar Baby” juice contain lycopene as antiinfl ammation. The aim of this research was to analyze the effect of watermelon as antiinfl ammation toward neutrophils count in wistar strain male rats exposed by  cigarette smoke. This study was an experimental laboratory research with pre and post test control group design. Sample consisted of 24 wistar strain male rats with ±150-230 grams weight and 3 months old which were divided into four groups. Control group was given placebo (aquadest) and treatment groups were given w atermelon juice with terraced quantity (1.5 ml, 2.5 ml, and 3.5 ml). Every group was given two sticks of cigarette smoked per day during two weeks then smoked with treatment during one week. Neutrophil from peripheral smear was observed by manual method and statistically analyzed using Anova Mixed Design and continued with One Way Anova. The result showed signifi cant increased of  neutrophil at second observation on control group (p=0.002) and treatment groups (p=0.000). Signifi cance decreased also found in the treatment groups’s at third observation p=0.000. Signifi cant between each group p= 0.017. There was signifi cant different between control and treatment groups but there was no signifi cant different between each treatment groups. This study concluded that there was decreased of  neutrophil in wistar strain male rats which were given w atermelon juice 1.5 ml, 2.5 ml, and 3.5 ml during infl ammation. 3.5 ml w atermelon juice which were given showed better result as antiinfl ammation..

scholarly journals The Effect of Hyperbaric Oxygen Therapy to the Amount of Lymphocytes in Oral Candidiasis Immunosuppressed Model

DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 36
Author(s):  
Agni Febrina Pargaputri ◽  
Dwi Andriani
Keyword(s):  
Immune System ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Wistar Rats ◽  
Oxygen Therapy ◽  
Oral Candidiasis ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
The Difference

<p><strong><em> </em></strong></p><p><strong><em>Background: </em></strong><em>Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. <strong>Purpose: </strong>To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. <strong>Methods:</strong> This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. <strong>Results:</strong> The data were analyzed with one way Anova test and showed significant difference among groups (p&lt;0,05). To show the difference between each groups we used LSD test and showed significant difference (p&lt;0,05) between K+ compared with P1, and K+ compared with K-. <strong>Conclusion:</strong> Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models.</em></p><p><em> </em><strong><em>Keywords:</em></strong><em> Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes</em></p><p> <strong><em>Correspondence</em></strong><strong><em>: </em></strong><em>Agni Febrina Pargaputri</em><em>, Departmen</em><em>t of</em><em> Oral </em><em>Biology </em><em>F</em><em>aculty of</em><em> </em><em>Dentistry</em><em>, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. </em><em>Phone:</em><em> 031-5945864, fax: 031-5912191, Email: </em><em><span style="text-decoration: underline;">agni.febrina</span></em><em><span style="text-decoration: underline;">@hangtuah.ac.id</span></em></p><p> </p>

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