scholarly journals Biochemical analysis of ginger-infused water with a combination of lemon and mint leaves

2021 ◽  
Vol 332 ◽  
pp. 06002
Author(s):  
Rika Sepriani ◽  
Deswandi
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Biochemical Analysis ◽  
Ph Value ◽  
A Value ◽  
Dpph Method ◽  
Red Ginger

Infused water is a beverage made possible by the incorporation of fruit or herbs into the water. The purpose of this study is to determine the antioxidant activity, vitamin C content, and pH value of ginger-infused water with lemon and mint leaves. This study used three ginger varieties: red ginger, small (emprit) ginger, and elephant ginger, with immersion times of 6, 9, and 12 hours. The DPPH method was used to determine antioxidant activity, and UV-Vis spectrometry was used to determine vitamin C levels. A pH meter was used to determine the degree of acidity (pH). According to the study’s findings, red ginger had the highest antioxidant activity at 12 hours of immersion by 89 percent. At 72.44 ppm immersion for 12 hours, the highest levels of vitamin C were found in infused water with small (emprit) ginger and a combination of lemon and mint leaves. Red ginger with a combination of lemon and mint leaves had the lowest pH, with a value of 3.48 after 12 hours of immersion. Research shows the biochemical content of infused water that is often used in society, especially ginger infused water combined with lemon and mint leaves.

scholarly journals FORMULASI DAN UJI EFEKTIVITAS ANTIOKSIDAN SEDIAAN KRIM EKSTRAK ETANOL DAUN SALAM (Syzygium Polyanthum (Wight) Walpers.) DENGAN MENGGUNAKAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 720
Author(s):  
Etserlisa A. Apitalau ◽  
Hosea Jaya Edy ◽  
Karlah L.R. Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Experimental Methods ◽  
Physical Test ◽  
A Value ◽  
Activity Test ◽  
Test Parameters ◽  
Dpph Method ◽  
The Impact

ABSTRACTBay leaf (Syzygium Polyanthum (Wight) Walpers.) is known contains flavonoids, saponins, tannins and vitamin C. Flavonoids and vitamin C have an antioxidant activity, since they have the ability to remove and effectively reduce a damaging oxidizer species. The aim of the study is to determine the impact of  the bay leaf ethanol extract enhancement concentration towards  an antioxidant activity of cream preparation by using the DPPH method. This research used laboratory experimental methods. Cream formulations are made with various concentrations 1%, 3%, 6% and 9%. The evaluation results of the bay leaf cream preparation has fulfilled the requirements of homogeneity, organoleptic, pH, adhesion, dispersion, and centrifugation. The results of the antioxidant activity test using the DPPH method using the UV-Vis spectrophotometer which was the most effective as an antioxidant cream from bay leaf extract was 3% with a value IC_50 = 1.4630 ppm and vitamin C as a comparison with a value IC_50 = 0.1131 ppm. From this research, it can be concluded that the cream formulation of bay leaf ethanol extract has fulfilled the physical test parameters, stabile, and had a powerful antioxidant activity.Keywords: : Bay Leaf (Sysigium Polyanthum (Wight) Walpers.), Antioxidant Cream, DPPH (1.1-Diphenyl-2-Picrylhydrazyl) Method  ABSTRAKDaun salam (Syzygium Polyanthum (Wight) Walpers.) diketahui mengandung flavonoid, saponin, tanin dan vitamin C. Senyawa flavonoid dan vitamin C memiliki aktivitas sebagai antioksidan, karena memiliki kemampuan untuk menghilangkan dan secara efektif mengurangi spesies pengoksidasi yang merusak. Penilitian ini bertujuan untuk mengetahui pengaruh peningkatan kosentrasi ekstrak etanol daun salam terhadap aktivitas antioksidan pada sediaan krim dengan menggunakan metode DPPH. Penelitian ini mengggunakan metode eksperimental laboratorium. Formulasi sediaan krim dibuat dengan variasi konsentrasi 1%, 3%, 6% dan 9%. Hasil evaluasi sediaan krim daun salam memenuhi persyaratan homogenitas, organoleptis, pH , daya lekat, daya sebar, sentrifugasi. Hasil uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometer UV-Vis yang paling efektif sebagai krim antioksidan dari ekstrak daun salam adalah 3% memiliki nilai = 1,4630 ppm dan vitamin C sebagai pembanding memiliki nilai = 0,1131 ppm. Dari penilitian ini dapat disimpulkan bahwa sediaan krim ekstrak etanol daun salam memenuhi parameter uji fisik, stabil dan memiliki aktivitas antioksidan yang sangat kuat. Kata kunci : Daun Salam (Sysigium Polyanthum (Wight) Walpers.) Krim Antioksidan, Metode DPPH (1.1-Diphenyl-2-Picrylhydrazyl)

scholarly journals FORMULASI DAN UJI EFEKTIVITAS ANTIOKSIDAN SEDIAAN KRIM EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L. ) DENGAN MENGGUNAKAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 501
Author(s):  
Feiby Ariem ◽  
Paulina V.Y Yamlean ◽  
Julianri Sari Lebang
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Test Results ◽  
Cycling Test ◽  
A Value ◽  
Dpph Method ◽  
Before And After

ABSTRACTBelimbing wuluh leaves (Averrhoa bilimbi L.) Contains  flavonoids, phenols, alkaloids, tannins and coumarin. The aim of this research is formulated ethanol extract of Belimbing Wuluh leaves in the form of cream preparations, as well as test the effectiveness of antioxidants using the DPPH method. Extract was made by maceration method using ethanol 96% formulated into a cream with a concentration of 3%, 5%, 7%. The results of the cream preparations evaluation before and after cycling test meet the requirements of organoleptis, homogeneity, pH, adhesiveness, coverage. The test results of antioxidant activity with the DPPH method using the most effective UV-Vis spectrophotometer as the antioxidant cream of the leaves extract Belimbing Wuluh is 7% has a value of IC50 = 0.118 ppm and vitamin C as comparator has a value of IC50 = 0.0327 ppm. From this research can be concluded that Belimbing Wuluh leaves ethanol extract cream can be formulated as a stable cream and has a very strong antioxidant activity. Keywords: : Belimbing Wuluh Leaves,cream, antioxidant,DPPH ABSTRAK Daun Belimbing Wuluh (Averrhoa bilimbi L.)  mengandung senyawa flavonoid, fenol, alkaloid, tanin dan kumarin. Tujuan penelitian ini membuat formulasi ekstrak etanol daun Belimbing Wuluh dalam bentuk sediaan krim, serta menguji efektivitas antioksidan menggunakan metode DPPH. Pembuatan ekstrak dilakukan dengan metode maserasi menggunakan etanol 96 % diformulasi menjadi krim dengan konsentrasi 3%, 5% ,7%. Hasil evaluasi sediaan krim sebelum dan sesudah cycling test memenuhi persyaratan organoleptis, homogenitas, pH, daya lekat, daya sebar. Hasil uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometer UV-Vis yang paling efektif sebagai krim antioksidan dari ekstrak daun Belimbing Wuluh adalah 7 % memiliki nilai IC50 = 0,118 ppm dan vitamin C sebagai pembanding memiliki nilai IC50 = 0,0327 ppm. Dari penelitian ini dapat disimpulkan bahwa krim ekstrak  etanol daun Belimbing Wuluh dapat diformulasikan sebagai sediaan krim yang stabil dan memiliki aktivitas antioksidan yang sangat kuat.  Kata kunci : Daun Belimbing Wuluh, Krim, Antioksidan, DPPH

Optimization of the Extraction Technology and Assessment of Antioxidant Activity of Chlorogenic Acid-Rich Extracts From Eucommia ulmoides Leaves

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110461
Author(s):  
Hua Jiang ◽  
Jun Li ◽  
Ning Zhang ◽  
Hai-Yang He ◽  
Jia-Min An ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorogenic Acid ◽  
Ph Value ◽  
Extraction Process ◽  
Eucommia Ulmoides ◽  
Extraction Technology ◽  
Dpph Method ◽  
Contour Plots ◽  
Technology And Assessment ◽  
Design Optimum

Chlorogenic acid has been proved to have cardiovascular protection, antibacterial, antiviral, hemostatic, and hypolipidemia effects. Modern scientific research on the bioactivity of chlorogenic acid has been extended to the fields of food, medicine, health care and daily-use chemical industry. The aim of this research was to optimize the extraction conditions for chlorogenic acid from Eucommia ulmoides (Eucommiaceae) leaves. The significant variables were screened and optimized by a combination of Plackett-Burman test and Box-Behnken design. Optimum extraction parameters with ethanol concentration of 50%, solvent pH value of 3, and particle size of 60 mesh were determined according to variance analysis and contour plots. Under these conditions, the yield of chlorogenic acid was up to 4.36 mg/g, which was basically consistent with the theoretical prediction value of 4.50 mg/g. This study also proved the potential antioxidant activity of E. ulmoides leaves. The optimal extract of E. ulmoides leaves rich in chlorogenic acid showed the highest antioxidant activity in the FRAP method, which was 219.8 μM Trolox equivalents (TE) per g extract weight (EW) (μM TE/g EW). The DPPH method gave a similar value (168 μM TE/g EW) to the ABTS method (152 μM TE/g EW). The established extraction process was efficient in the recovery of chlorogenic acid from E. ulmoides leaves, encouraging its valorization as a cheap and sustainable alternative for the isolation of chlorogenic acid.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL SPONS Ianthella basta DARI DESA TUMBAK KECAMATAN PUSOMAEN KABUPATEN MINAHASA TENGGARA

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 713
Author(s):  
Nurhati Anton ◽  
Adithya Yudistira ◽  
Jainer Pasca Siampa
Keyword(s):  
Antioxidant Activity ◽  
Coral Reef ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Positive Control ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACT Sponges are a component of coral reef biota that has bioactive compounds with a greater percentage of activity compared to compounds produced by terrestrial plants. This study aims to determine the presence of antioxidant activity in the ethanol extracts of sponge Ianthella basta from the waters of Tumbak Village. Ianthella basta sponge was extracted using maceration method with 96% ethanol solvent. Antioxidant activity testing was carried out using the DPPH method with a concentration of 100 µg / mL, 75 μg / mL, 50 μg / mL, and 25 μg / mL and Vitamin C p.a as positive control. Each sample was made three repetitions of the test. Testing using a UV-Vis spectrophotometer. The results showed that the ethanol extract of the Ianthella basta sponge had antioxidant activity with a percentage of 48.73% at a concentration of 100 µg / mL. Keywords: Ianthella basta sponge, Antioxidants, Extraction, DPPH  ABSTRAK Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai senyawa bioaktif dengan persentase keaktifan lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan di dalam ekstrak etanol Spons Ianthella basta dari perairan Desa Tumbak. Spons Ianthella basta diekstraksi menggunakan metode maserasi dengan pelarut etanol 96%. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH dengan konsentrasi 100 µg/mL, 75 µg/mL, 50 µg/mL, dan 25 µg/mL dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ektrak etanol Spons Ianthella basta memiliki aktivitas antioksidan dengan persentase sebesar 48,73% pada konsentrasi 100 µg/mL. Kata Kunci: Spons Ianthella basta, Antioksidan, Ekstraksi, DPPH

scholarly journals Uji Kualitatif dan Uji Aktivitas Antioksidan Ekstrak Etanolik Buah Maja (Aegle Marmelos (L.)Correa) dengan Metode DPPH

2021 ◽  
Vol 1 (1) ◽  
pp. 1-8
Author(s):  
Muhammad Nur Fauzi
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Aegle Marmelos ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
The Third ◽  
Dpph Method ◽  
Fruit Samples ◽  
The World

Abstract. Indonesia is a country with the third largest tropical forest in the world. The number of medicinal plants in Indonesia is estimated to be around 1,260 types of plants. Plants produce secondary metabolites that have potential as antioxidants. One of the plants that contains a lot of secondary metabolites is maja (Aegle marmelos (L.) Corr). The purpose of this study was to determine the content of secondary metabolites in maja fruit extracts and to determine the antioxidant activity contained in maja fruit extracts using the DPPH method. The research was conducted by extracting maja fruit samples using maceration method to obtain a thick extract. The extracts obtained were tested for secondary metabolites, TLC test, and antioxidant activity tests using the DPPH method using Uv-vis spectrophotometry. The results of this study indicate that maja fruit extract contains secondary metabolites of flavonoids, tannins, alkaloids, saponins, and glycosides. TLC results obtained Rf 0.512. The result of antioxidant activity of maja fruit extract obtained by IC50 was 269.153 µg / mL. and IC50 vitamin C as a comparison obtained 28,907µg / mL. This shows that the antioxidant activity of maja fruit extract is smaller than the antioxidant activity of vitamin C. Abstrak. Indonesia adalah negara dengan hutan tropis paling besar ketiga di dunia, Jumlah tumbuhan berkhasiat obat di Indonesia diperkirakan sekitar 1.260 jenis tumbuhan. Tumbuhan menghasilkan metabolit sekunder yang berpotensi sebagai antioksidan. Salah satu tanaman yang banyak mengandung metabolit sekunder adalah tanaman maja (Aegle marmelos (L.) Corr). Tujuan dari penelitian ini adalah untuk mengetahui kandungan metabolit sekunder pada ekstrak buah maja dan untuk mengetahui aktivitas antioksidan yang terdapat pada ekstrak buah maja dengan metode DPPH. Penelitian dilakukan dengan mengekstraksi sampel buah maja dengan metode maserasi untuk memperoleh ekstrak kental. Ekstrak yang diperoleh dilakukan uji metabolit sekunder, uji KLT, dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri Uv-vis. Hasil penelitian ini menunjukkan bahwa ekstrak buah maja mengandung metabolit sekunder flavonoid, tanin, alkaloid, saponin, dan glikosida. Hasil KLT diperoleh Rf 0,512. Hasil aktivitas antioksidan ektrak buah maja yang diperoleh dengan IC50 adalah 269,153 µg/mL. dan IC50 vitamin c sebagai pembanding diperoleh 28,907µg/mL. hal ini menunjukan bahwa daya aktivitas antioksidan ekstrak buah maja lebih kecil dibanding dengan daya aktivitas antioksidan vitamin C. Kata Kunci: , , 

scholarly journals Effects of Different Weather Conditions and Addition of Mangosteen Peel Powder on Chemical Properties and Antioxidant Activity of Coconut Sap

2019 ◽  
Vol 38 (3) ◽  
pp. 295
Author(s):  
Pepita Haryanti ◽  
Supriyadi Supriyadi ◽  
Djagal Wiseso Marseno ◽  
Umar Santoso
Keyword(s):  
Antioxidant Activity ◽  
Ph Value ◽  
Chemical Properties ◽  
Weather Condition ◽  
Weather Conditions ◽  
Reducing Sugar ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Mangosteen Peel ◽  
Dpph Method

Coconut sap is sweet, oyster-white and translucent liquid which can be obtained by tapping coconut flowers for 8  ̶  12 hours. Since it contains sugar with nearly neutral pH, coconut sap is highly susceptible to spontaneous fermentation. In order to maintain the quality of the sap during the tapping process, coconut farmers usually add preservative substances. The preservatives can be produced either naturally, such as mixtures of lime, mangosteen peel and sliced jackfruit wood, or synthetic one, such as sodium metabisulphite. The chemical properties of coconut sap might be affected by weather conditions during the tapping process. This research aimed to determine the effect of weather conditions during tapping process and variations of mangosteen peel powder concentration on the chemical properties and antioxidant activity of the coconut sap. The results showed that the optimum preservative concentration of mangosteen peel powder was 0.84 g/L of coconut sap which was tapped in sunny weather condition. This treatment yielded sap with chemical properties i.e. pH value of 6.7; total soluble solids of 16.2 °brix; water content of 85.62%; reducing sugar of 0.04 g/100 g; sucrose content of 13.64 g/100 g; total free amino acids of 0.1738 g/100 g; total phenolic of 323.23 mgGAE/100 g; and vitamin C of 26.06 mg/100 g. The antioxidant activity of this treatment was 34.39 % RSA evaluated by DPPH method, this value, was relatively similar to that of the addition of 0.28 and 0.56 g/L mangosteen peel powder. The coconut sugar produced had reducing sugar of 5.01 g/100 g; total sugar of 86.50 g/100 g; total phenolic content of 470 mgGAE/100 g; browning index of 16254 IU (ICUMSA); and antioxidant activity of 61.05% RSA.

scholarly journals Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Fitmawati Fitmawati ◽  
Nery Sofiyanti ◽  
Rodesia Mustika Roza ◽  
Isnaini Isnaini ◽  
Yulisa Resti Irawan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Vitamin C ◽  
Natural Resources ◽  
The Other ◽  
Distilled Water ◽  
Water Solvent ◽  
Activity Test ◽  
Dpph Method ◽  
Riau Archipelago

<p><em>Obat Pahit</em> is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely <em>Bauhunia semibifida, Cnestis palala</em> and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in <em>Obat Pahit</em> from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of <em>Obat Pahit</em> from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of <em>Bauhinia semibifida</em> (6.6247), Penawa Root (5.0124) and <em>Cnestis palala</em> (5.9968) were much lower than IC50 of mangosteen’s rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesia’s local ethnicities.</p>

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG MAHONI (Swietenia mahagoni Jacq.) MENGGUNAKAN METODE DPPH (1,1 diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 548
Author(s):  
Amanda T. M. Turangan ◽  
Defny S. Wewengkang ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Medicinal Plant ◽  
Positive Control ◽  
Antioxidant Compounds ◽  
Phytochemical Screening ◽  
Oxidation Reactions ◽  
Dpph Method ◽  
Swietenia Mahagoni

Antioxidant are compounds that are able to inactivate the development of the oxidation reactions of the molecules or neutralize free radicals. Mahogany bark (Swietenia mahagoni Jacq.) has properties as a medicinal plant. Through the phytochemical screening, the mahogany bark (Swietenia mahagoni Jacq.) contains antioxidant compounds such as alkaloids, saponins and phenolics. The aim of this study was to determine the presence of antioxsidant activity in the extract of mahogany bark (Swietenia mahagoni Jacq.). using the DPPH method with a concentration of 250 mg / L, 200 mg / L, 150 mg / L and 100 mg / L and Vitamin C p.a as a positive control. Each sample made three repetitions of the test. The test uses a UV-Vis Spectrophotometer. The result of the study showed that mahogany bark (Swietenia mahagoni Jacq.) showed antioxidant activity with the percentage of 21,50% at the concentration at 250 mg / LKeywords : Swietenia mahagoni Jacq., Antioxidant, Ethanol, DPPHABSTRAKAntioksidan ialah senyawa yang mampu menginaktivasi berkembangnya reaksi oksidasi molekul lain atau menetralisir radikal bebas. Kulit Batang Mahoni (Swietenia mahagoni Jacq.) memiliki khasiat sebagai tanaman obat. Melalui skrining fitokimia, Swietenia mahagoni Jacq. Mengandung senyawa antioksidan seperti alkaloid, saponin dan fenolik. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan didalam ekstrak tanaman Kulit Batang Switenia mahagoni Jacq. menggunakan metode DPPH dengan konsentrasi 250 mg/L, 200 mg/L, 150 mg/L dan 100 mg/L dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat Spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak Kulit Batang Swietenia mahagoni Jacq. memiliki aktivitas antioksidan dengan presentase sebesar 21,50% pada konsentrasi 250 mg/L Kata Kunci : Swietenia mahagoni Jacq., Antioksidan, Etanol, DPPH

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