scholarly journals Dietary Supplementation Effects of Noni (Morinda Citrifolia L.) Fruit Flour on Uric Acid and Blood Glucose of Quails (Coturnix Coturnix Japonica) Layer Phase

2017 ◽  
Vol 2 (6) ◽  
pp. 80
Author(s):  
Lovita Adriani ◽  
Bigtha Yeane
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Animal Husbandry ◽  
Basal Diet ◽  
Experimental Period ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Blood Uric Acid

This study was aimed to determine the effect of noni fruit flour in ration on  uric acid and blood glucose of  quails layer  phase. The research was conducted from  March to May 2015 at  Faculty of Animal Husbandry Universitas Padjadjaran. One hundred commercialquails aged 4 weeks were randomly allocated to four treatment groups as T1, T2, T3 and T4. The quails  in the control group (T1) were given normal basal diet without the addition of noni fruit flour, while as other groups (T2, T3, T4) were supplemented with0.25, 0.50, and 0.75% noni fruit flour  respectively with 25 quails per treatment group replicated five times with five quails per replicate in a Complete Randomized Design (CRD). The blood samples were randomly collected, five birds per replicate at the end experimental period (7th week) and analyzed for the estimation of uric acid and blood glucose. The results revealed that blood glucose was significantly (p>0.05) increased in the groups fed noni at various levels when compared to the control. Further, a significantly (p<0.05) proportional decreasing  in blood uric acid levels was found with increase in the level of dietary  noni fruit flour, with highest reduction in the group supplemented with 0.5% noni fruit flour (T3) compared to the control group and in the group suplemented with 0.25% (T2) for blood glucose. In conclusion, dietary inclusion of noni fruit flours had beneficial effect with regard to its ability in reducing the blood uric acid levels and increasing blood glucose of quails. Keywords: Quail, uric acid, blood glucose, Noni

scholarly journals Effect of different levels of L-carnitine and lysine-methionine on broiler blood parameters

2015 ◽  
pp. 4698-4708 ◽  
Author(s):  
Babak Hosseintabar ◽  
Mohammad Dadashbeiki ◽  
Mehrdad Bouyeh ◽  
Alireza Seidavi ◽  
René Van den Hoven ◽  
...  
Keyword(s):  
Uric Acid ◽  
Total Cholesterol ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Blood Parameters ◽  
Control Group ◽  
Blood Concentrations ◽  
Treatment Groups ◽  
Para Determinar ◽  
Different Levels

ABSTRACT Objetive. In the present study a completely randomized 3×3 factorial design was used to analyze the effects of different levels of L-Carnitine, lysine(Lys) and methionine (Met) on the blood concentrations of energy, protein and lipid metabolites of male broiler chickens. Materials and methods. A total of 270 newly hatched male broiler chickens (Ross 308) were randomly assigned to 9 groups (ten broilers per replicate and three replicates per treatment). The control group was fed a basal diet, whereas the treatment groups were fed basal diets supplemented with L-Carnitine (0 mg/kg, 75 mg/kg and 150 mg/kg) and lysine-methionine (0, 15 and 30%) for 42 days. On day 42, one bird was randomly chosen per replication, a blood sample was taken and the blood concentrations of glucose (GLU), uric acid (UAc), triglyceride (TG), VLDL, HDL, LDL, total protein (TP), albumin (Alb) and total cholesterol (TC) were analyzed. Results. Dietary L-carnitine supplementation had a significant effect (p<0.05) on uric acid (UAc), HDL, LDL, and total cholesterol (TC). The birds feed L-carnitine plus Lys and Met presented the highest plasmatic UAc level and the lowest plasmatic TC and LDL level. Moreover, L-carnitine significantly reduced total cholesterol (TC) when compared with both the control group and the birds feed Lys and Met without L-carnitine. Conclusions. A diet with 150 mg/kg L-carnitine plus 15% Lys and Met seems to be enough to sustain low plasmatic TC, LDL and HDL concentrations on male broiler. RESUMEN Objetivo. Se realizó un estudio para determinar el efecto de la suplementación deL-carnitina, lisina (Lys) y metionina (Met) sobre los metabolitos sanguíneos de pollos de engorde. Materiales y métodos. Se utilizaran 270 pollos de la línea Ross 308 de un día de edad y se dividieron en 9 tratamientos en un diseño al azar con arreglo factorial 3x3: tres niveles de L-carnitina (0 mg/kg, 75 mg/kg y 150 mg/kg) y tres de lisina-metionina (0, 15 y 30%) durante 42 días. Cada tratamiento constó de 3 repeticiones con 10 pollos por repetición. El día 42 de edad, se tomaron muestras de sangre de tres aves por tratamiento para cuantificar niveles séricos de glucosa (GLU), ácido úrico (UAc), triglicéridos (TG), VLDL, HDL, LDL, proteínas totales (PT), albúmina (Alb) y colesterol total (TC). Resultados. La suplementación de L-carnitina en la dieta tuvo un efecto significativo (p<0.05) en los niveles de ácido úrico en suero (UAC), HDL, LDL y colesterol total (CT). Las aves alimentadas con L-carnitina más Lys y Met mostraron niveles séricos más altos de UAc y menor TCy LDL. Por otra parte, la L-carnitina redujó significativamente el colesterol total (CT), cuando se comparó con el grupo control y con los pollos alimentados con Lys y Met, sin L-carnitina. Conclusiones. Una dieta con 150 mg/kg de L-carnitina y 15% Lys y Met parece ser suficiente para mantener bajas concentraciones plasmáticas de TC, LDL y HDL en pollos de engorde.

scholarly journals Effect of adding L-carnitine and Protexin(R) probiotic on performance and some blood parameters of ostrich chickens

2016 ◽  
Vol 3 (5) ◽  
pp. 153 ◽  
Author(s):  
Rozbeh Fallah ◽  
Enayat Mirzaei
Keyword(s):  
Blood Glucose Concentration ◽  
Basal Diet ◽  
Blood Parameters ◽  
The Other ◽  
Control Group ◽  
Feed Conversion ◽  
Treatment Groups ◽  
Randomized Design ◽  
Dietary Treatments ◽  
Diet Supplementation

This study was conducted to investigate the effects of adding L-carnitine and Protexin(R) probiotic on performance and some blood parameters of ostrich chickens. In this study, a total of 48 one-day old ostrich chicks randomly divided into four treatment groups. Each treatment comprised of 4 replicates and 3 animals in each replicate and reared for 8 week. The experiment was arranged in a completely randomized design. The dietary treatments were: Treatment 1 (Control): Basal diet without supplementation; Treatment 2: Protexin(R) probiotic with level of 1 kg/t; Treatment 3: L-carnitine 500 mg/kg diet; Treatment 4: Protexin(R) probiotic with level of 500 kg/t + L-carnitine 250 mg/kg diet. The results of this study indicated that diet supplementation with L-carnitine and Protexin(R) probiotic increased ostrich chickens weight and feed intake at 8 weeks of age in comparison to other treatment groups. The other results showed no significant differences in feed conversion ratio (FCR) among different treatments at 8 week of ages and Broilers receiving L-carnitine + Protexin(R) probiotic had lowest FCR compared to the other tratment groups. Furthermore, diet supplementation with L-carnitine and Protexin(R) probiotic increased blood glucose concentration of ostrich chickens at 8 weeks of age in comparison to other treatment groups. Futher result showed that birds received L-carnitine + Protexin(R) probiotic had lowest total cholesterol in compersion to other treatment groups and birds receiving L-carnitine and Protexin(R) probiotic had lower HDL, LDL, and VLDL concentrations in compersion control group. There were no significant effect in calcium and phosphorus concentrations between control and other treatment groups.

PENGARUH PEMBERIAN KOMBINASI EKSTRAK BUAH MENGKUDU (Morinda citrifolia L.) DAN KUNYIT (Curcuma longa) TERHADAP HISTOPATOLOGI GINJAL TIKUS WISTAR YANG DIINDUKSI ALLOXAN

2020 ◽  
Vol 5 (2) ◽  
pp. 319-327
Author(s):  
Pelastri Rahayu ◽  
◽  
Retno Hestiningsih ◽  
Martini Martini ◽  
Dwi Sutiningsih ◽  
...  
Keyword(s):  
Body Weight ◽  
Diabetic Nephropathy ◽  
Blood Glucose ◽  
Curcuma Longa ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Type I ◽  
Turmeric Extract ◽  
Measurement Results ◽  
Diabetes Nephropathy

The prevalence of DM in Riskesdas in 2018 according to the Perkeni consensus in 2015 is higher than according to the Perkeni consensus in 2011, the prevalence was10.9%. The disease can develop into diabetes nephropathy, Increased prevalence of diabetic nephropathy directly proportional with an increase in diabetes prevalence. Diabetic nephropathy is a microvascular complication in diabetics that develops around 30% in patients with type I DM and about 40% in patients with type II DM. Turmeric extract has antioxidant and anti-inflammatory effects to prevent the bad development of diabetes nephropathy. This study looked at the effect of giving a combination of noni and turmeric extract on histopathology of alloxan-induced renal rats. A total of 25 mice were divided into 5 treatment groups, namely the PI group (250 mg / kgBB extract dose), PII group (500 mg / kgBB extract dose), PIII group (750 mg / kgBB extract dose), positive control group (glibenklamid) and negative control group (without extract and glibenklamid). The study used Post Test Only Group. The highest percentage decrease in blood glucose in the PI group was 56.11% and the lowest decrease in the PIII group was 24.12% with p = 0.012. The results of the study were not based on the number of extract doses. The measurement results of rat body weight and glomerular diameter were not affected by blood glucose level with p = 0.700 for body weight and p = 0.187 for glomerular measurement results.

scholarly journals Dietary purslane (Portulaca oleracea L.) promotes the growth performance of broilers by modulation of gut microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cong Wang ◽  
Qing Liu ◽  
Fengchun Ye ◽  
Hongbo Tang ◽  
Yanpeng Xiong ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Growth Performance ◽  
Basal Diet ◽  
Scientific Basis ◽  
Control Group ◽  
Good Growth ◽  
Feed Conversion ◽  
Illumina Hiseq ◽  
Treatment Groups ◽  
Dietary Treatments

AbstractPurslane is a widespread wild vegetable with both medicinal and edible properties. It is highly appreciated for its high nutritional value and is also considered as a high-quality feed resource for livestock and poultry. In this study, Sanhuang broilers were used to investigate the effect of feeding purslane diets on the growth performance in broilers and their gut microbiota. A total of 48 birds with good growth and uniform weight were selected and randomly allocated to four treatment groups A (control), B, C and D. Dietary treatments were fed with basal diet without purslane and diets containing 1%, 2% and 3% purslane. The 16S rDNA was amplified by PCR and sequenced using the Illumina HiSeq platform to analyze the composition and diversity of gut microbiota in the four sets of samples. The results showed that dietary inclusion of 2% and 3% purslane could significantly improve the growth performance and reduce the feed conversion ratio. Microbial diversity analysis indicated that the composition of gut microbiota of Sanhuang broilers mainly included Gallibacterium, Bacteroides and Escherichia-Shigella, etc. As the content of purslane was increased, the abundance of Lactobacillus increased significantly, and Escherichia-Shigella decreased. LEfSe analysis revealed that Bacteroides_caecigallinarum, Lachnospiraceae, Lactobacillales and Firmicutes had significant differences compared with the control group. PICRUSt analysis revealed bacteria mainly enriched in carbohydrate metabolism pathway due to the additon of purslane in the diet. These results suggest that the addition of purslane to feed could increase the abundance of Lactobacillus in intestine, modulate the environment of gut microbiota and promote the metabolism of carbohydrates to improve its growth performance. This study indicates that the effect of purslane on the growth-promoting performance of broilers might depend on its modulation on gut microbiota, so as to provide a certain scientific basis for the application of purslane in the feed industry.

PSVII-23 Experimental evaluation of the effectiveness of the Quercus cortex extract in the carp feeding system

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 339-339
Author(s):  
Mariya Miroshnikova ◽  
Elena Miroshnikova ◽  
Alexey Sizentsov ◽  
Azamat Arinzhanov ◽  
Yuliya Kilyakova
Keyword(s):  
Calcium Content ◽  
Fish Farming ◽  
Basal Diet ◽  
Experimental Period ◽  
Atomic Emission ◽  
Essential Elements ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Microbial Profile ◽  
Combined Feed

Abstract One of the most promising ways to improve the effectiveness of fish farming is the use of phytobiotics in the diet of fish. On this basis, we set the aim to evaluate the effectiveness of the Quercus cortex extract in the concentration of 1 mg/kg on biological features and the productivity of carp. The object of the research was yearling carp grown in the conditions of Orenburgskiy Osetr LLC. Two groups (n = 20) were formed by the method of analogs to conduct the research. After the adaption period (7 days), the groups came to the experimental period (35 days). The feed KRK-110–1 produced by PJSC Orenburg Combined-Feed Plant was used as the basal diet. The live fish were monitored weekly by individual weighing during the accounting period. The elemental composition was determined by the method of atomic emission and mass spectrometry (ICP-AES and ICP-MS). The microflora analysis was carried out by the method of metagenomic sequencing. The use of the Quercus cortex extract in the diet in the studied concentration increased body weight by 15.1% (P ≤ 0.05) compared to the control group. There was experimentally revealed a stimulating effect on the population growth of individual representatives of the microbiome (Luteolibacter, Lactococcus) (P ≤ 0.05) without significantly changing the overall picture of the microbial profile, which, in our view, affects the metabolic processes, in particular, by stimulating the formation of biologically available forms of essential elements and their subsequent accumulation in the tissues of the studied fish. Thus, the experimental group found: (against the background of an increase in the total mineralization (ash residue) by 17.95 % (P ≤ 0.05)) the calcium content increased by 133.9% (P ≤ 0.05), phosphorus by 83% (P ≤ 0.05), iron by 337.7% (P ≤ 0.05), respectively, in comparison with the control group.

scholarly journals Pengaruh Ekstrak Rebusan Daun Tithonia diversifolia (Hemsl.) A. Gray Terhadap Kadar Glukosa Darah

2019 ◽  
Vol 7 (1) ◽  
pp. 41
Author(s):  
Rinawati Rinawati ◽  
E Suharyanto ◽  
Nastiti Wijayanti
Keyword(s):  
Blood Glucose ◽  
Young Leaf ◽  
Tithonia Diversifolia ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Randomized Design ◽  
Adult Leaf ◽  
Reduce Blood Glucose ◽  
Completely Randomized Design

Tithonia diversifolia is a plant that has the potential to reduce blood glucose levels that is crucial in Diabetes Mellitus therapy. This study aims to determine the active compounds contained in the extract of T. diversifolia leaves decoction, the effect of decoction extract on the reduction of blood glucose, and the most effective decoction extract to reduce blood glucose levels as well. The study used a Completely Randomized Design (CRD) with the research treatment groups, namely: Kn = normal control (normal mice and not given leaves decoction extract), Ka = STZ control (DM mice, which are normal mice induced by 65mg/Kg of STZ), Kp = treatment control (normal mice given leaf decoction extract) , P1 = DM + mice were given young leaf decoction extract, P2 = DM + mice were given a mixture of young leaf and adult leaf decoction extract, P3 = DM + mice were given adult leaf decoction extract. The leaves used for decoction were 1-6 leaves from the shoots. The analysis of T. diversifolia leaves decoction compounds is using the visible spectrophotometers for tannins, phenols and flavonoids and GC-MS for terpenoid analysis. The result found that Tithonia diversifolia leaves decoction extracts contain tannins, flavonoids and phenols, while terpenoids are not detected. The leaves decoction extracts of T. diversifolia influenced the decreases in blood glucose levels of DM mice, especially the decoction extract of adult leaves which reduced blood glucose levels to 71.16%.

Effects of Compound Probiotics on Growth Performance, Intestinal Microflora and Immune Function of Broilers

2014 ◽  
Vol 997 ◽  
pp. 178-184
Author(s):  
Hong Ling Wang ◽  
Yu Hong Yang ◽  
Jia Qing Wang ◽  
Zhuo Wang ◽  
Shuang Ma
Keyword(s):  
Immune Function ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Intestinal Microflora ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Group Iii ◽  
Serum Biochemical ◽  
Anti Body

This study aimed to investigate the efficacy of compound probiotics on broiler performance, intestinal microflora, immune function and serum biochemical indicators. Four hundred one-d-old female broiler chickens were randomly divided into 4 groups with 4 replicates in each group and 25 chickens per replication. The chickens in groupI(control group) were fed a basal diet, experimental groups II, III and IVwere fed the basal diet supplemented with 0.1%, 0.2% and 0.4% compound probiotics. The results of this work were as follows: (1) during the experimental period, compared with control group, the average daily in group III was significantly increased (P<0.05), the feed/gain and average daily feed intake were significantly lower (P<0.05); (2) at the age of 14 and 28 days, the number of cecal Lactic acid in group II and III were significantly higher than the control (P<0.05) and the content of E.coli has the opposite results. (3) the anti-body titer in group III was the highest, and significantly higher compared to the control (P<0.05), but there were no significantly differences between group IV and control group (P>0.05). (4) serum biochemical indexes of broilers in group III and IVwere significantly increased than that in control group (P<0.05). In conclusion, the supplementation of 0.2% compound probiotics could promote the growth performance, improve the cecal mico-ecosystem, enhance the anti-body titer and biochemical indexes in serum.

Transcriptomics and Transmission Ultrastructural Examination Reveals the Nephrotoxicity of Cadmium in Laying Hens

2021 ◽  
Author(s):  
Man Zhao ◽  
Wenbo He ◽  
Can Tao ◽  
Beiyu Zhang ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Laying Hens ◽  
Basal Diet ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Renal Tubules ◽  
Rna Seq ◽  
Ultrastructural Examination ◽  
Treatment Groups ◽  
Kegg Pathway Analysis ◽  
Transcriptome Expression

Abstract The objective of this study was to reveal the effects of cadmium (Cd) on ultrastructural changes, oxidative stress, and transcriptome expression in the kidneys of laying hens. Seventy-two healthy Hy-Line brown laying hens at 41 weeks old were randomly allocated to four treatment groups with six replicates. The control group received a basal diet without additional Cd incorporation, and the other three treatment groups received diets supplemented with 15, 30, or 60 mg Cd /kg of feed. After 6 weeks of exposure the results show that administration of 60 mg/kg Cd significantly reduced (P < 0.05) eggshell thickness. With an increase in the Cd concentration in feed, the concentrations of renal Zn, Fe also had changed. Renal histopathology and ultrastructure also showed aggravated damage to glomeruli and renal tubules, and the deformation of nuclei and mitochondria in all Cd treatment groups. With an increase in Cd in feed, the activity of GPX and CAT was significantly reduced (P＜0.05), while the activity of T-AOC was decreased (P＜0.05) only in the 60 mg/kg Cd group. RNA-seq analysis revealed that 410 genes displayed differential expression (≥ 1.5-fold) in the 60 mg/kg supplementation group, compared to the control group. GO and KEGG pathway analysis results showed that Cd affected many genes involved in mitochondria and ion transport. In conclusion, this study elaborates the mechanisms underlying renal toxicity caused by Cd, which might provide target candidate genes for alleviating Cd poisoning in laying hens.

Serum apolipoprotein A-1 is related to inflammatory factors in the acute phase of gout

2020 ◽  
Author(s):  
Baoyu Zhang ◽  
Yuan Wang ◽  
Xiaoxia Jia ◽  
Lin Mu ◽  
Xiaobo Wang
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Acid Level ◽  
Serum Uric Acid Level ◽  
Blood Lipid ◽  
Inflammatory Factors ◽  
Control Group ◽  
Apolipoprotein A ◽  
Increase Blood Glucose ◽  
Liver And Kidney

Abstract BACKGROUNDTo observe the correlation between serum apolipoprotein A-1(apoA-1) and inflammatory factors in patients with gouty arthritis. METHODSFrom February to September 2020, 97 patients with gout (gout group) and 70 healthy controls (control group) were selected as the study subjects，who were admitted to the outpatient department of Beijing lu he hospital affiliated to capital medical university.97 patients in the gout group were in the acute phase. Serum concentrations of apoA-1, NLRP3 inflammasome （NACHT-LRR-PYD protein 3,NLRP3), interleukin-1 (IL-1) and interleukin-9 (IL-9) were detected. The correlation of serum apoA-1 concentration, gout related inflammatory factors (NLRP3, IL-1 beta, IL-9) and other clinical and laboratory indicators was analyzed. RESULTSThe serum apoA-1 concentration in the gout group was lower than that in the control group (P<0.05). With the increase of serum uric acid level, serum apoA-1 concentration decreased（R2=-0.3160，P<0.05）. Multiple linear analyses were performed to increase blood glucose, blood lipid, liver and kidney, etc, and the correlation remained（OR=-3.36，P<0.05）. With the increase of serum IL-1 beta concentration, serum apoA-1 concentration decreased（R2=-0.3993，P<0.05）. Multiple linear analyses were performed to increase blood glucose, blood lipid, liver and kidney, etc., and the correlation remained（OR=-2.95，P<0.05）. CONCLUSIONSIn the acute stage of gout, as the serum uric acid level increases, the serum IL-1β concentration increases and the apoA-1 concentration gradually decreases, which may indicate that apoA-1 participates in the inflammatory response of gout to a certain extent.

Effects of probiotics-encapsulated live feed on growth and survival of juvenile Clarias batrachus (Linnaeus, 1758) after differential exposure to pathogenic bacteria

2018 ◽  
Vol 16 (1) ◽  
pp. 105-113 ◽  
Author(s):  
A Dey ◽  
K Ghosh ◽  
N Hazra
Keyword(s):  
Pathogenic Bacteria ◽  
Experimental Period ◽  
Clarias Batrachus ◽  
Control Group ◽  
Continuous Exposure ◽  
Protective Effects ◽  
Growth And Survival ◽  
Treatment Groups ◽  
Live Feed ◽  
Differential Exposure

Growth and survival of Clarias batrachus juveniles (10-day old) fed probiotic Bacillus cereus (KR809412) encapsulated live feed (chironomid larvae) have been evaluated after differential exposure to the pathogenic Aeromonas hydrophila (MTCC 1739). Catfish juveniles were stocked at a density of 30 fish per tank in five experimental groups (T1-T5) along with a control group in triplicate and fed twice @ 5% of body weight day-1 for four weeks. Groups T1 and T2 were fed probiotic-encapsulated (PR) or pathogen-inoculated (PGN) live feed respectively, for initial three weeks. During this period groups T3 (PGN-PR-PR), T4 (PR-PGN-PR), and T5 (PR-PR-PGN) were differentially exposed to the pathogen. Live feed without probiotic and pathogen was offered to the control group throughout the experimental period and all other treatment groups (T1-T5) during the 4th week. Continuous exposure to probiotics in group T1 resulted in significantly higher (P<0.05) specific growth rate (SGR, % d-1) and survivability than other groups, whereas, pathogen exposed and probiotic deprived group (T2) noticed with the lowest SGR and the highest mortality. Among other treatment groups (T3, T4 and T5), group T4 resulted in improved SGR and survivability. The coefficient (r value) of 0.867 along with regression slope suggested a positive correlation (0.01 levels) between RNA: DNA and SGR. The study might suggest protective effects of probiotic B. cereus in pathogen exposed C. batrachus juveniles.SAARC J. Agri., 16(1): 105-113 (2018)

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