Digestate as Biofertilizer for the Growth of Selected Vegetables and Illumina analysis of Associated Bacterial Community

ABSTRACTChemical content of crops above desirable level, high cost, in addition to land and water pollution is a major drawback of applying chemical fertilizers. In this study, Digestate was used as a biofertilizer for the growth of selected vegetables. Furthermore, Illumina platform was employed to unravel the bacteria community of the digestate. Corchorus olitorius, Amaranthus hybridus, Bot-celosia argentia and Talinum triangulare were grown in 16 experimental pots for 35days using cured digestate. Soil treated with chemical fertilizer was used as a positive control while the soil without any fertilizer was used as a negative control. The plant height of Talinum triangulare for soil treated with digestate was 23.5cm and 34cm by days 7 and 35 respectively after plant emergence. Corchorus olitorius had stunted growth under same treatment. Whereas, Bot-celosia argentia and Amaranthus hybridus grew poorly on all treatments. The statistical analysis showed a significant difference (p≤0.05) between Talinum triangulare grown in soil treated with digestate (plant height = 23.50, plant number =10 at day 7) compared with those treated with NPK (plant height = 18.50, plant number = 6.50 at day 7). The plant height and number for untreated soil at day 7 were 10.90 and 5.0 respectively). The Illumina sequencing of the digestate revealed the presence of some beneficial soil bacteria including Clostridium, Bacillus, Pseudomonas, Actinobacteria, and Micrococcus. The presence of these bacteria known to be Nitrogen fixers and Phosphate solubilizers confer biofertilizer potential to the digestate.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v2i2.59 ◽

2017 ◽

Vol 2 (2) ◽

pp. 147

Author(s):

Triswanto Sentat ◽

Susiyanto Pangestu

Keyword(s):

Analgesic Effect ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Control Treatment ◽

Treatment Groups ◽

Analgesic Effects ◽

Significant Difference ◽

Analgesic Dose ◽

Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

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Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00548 ◽

2021 ◽

pp. 3143-3149

Author(s):

Endang Sri Purwanti Ningsih ◽

Noorlaila Noorlaila ◽

Ikhwan Rizki Muhammad ◽

Windy Yuliana Budianto

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Leaf Extract ◽

Wound Care ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

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Evaluation of the Sealing Ability of Three Obturation Techniques Using a Glucose Leakage Test

BioMed Research International ◽

10.1155/2017/2704094 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Katarzyna Olczak ◽

Halina Pawlicka

Keyword(s):

Continuous Wave ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Thermal Methods ◽

Root Canals ◽

Anterior Teeth ◽

Sealing Ability ◽

Significant Difference

The aim of this study was to evaluate the sealing ability of three different canal filling techniques. Sixty-four roots of extracted human maxillary anterior teeth were prepared using ProTaper® rotary instruments. The specimens were then randomly divided into 3 experimental groups (n=16) and 2 control groups (n=8). The root canals were filled using cold lateral compaction (CLC group), continuous wave condensation technique using the Elements Obturation Unit® (EOU group), and ProTaper obturators (PT group). For the negative control group, 8 roots were filled using lateral compaction as in the CLC group, and the teeth were covered twice with a layer of nail varnish (NCG group). Another 8 roots were filled using lateral compaction, but without sealer, and these were used as the positive control (PCG group). A glucose leakage model was used for quantitative evaluation of microleakage for 24 hours and 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 weeks. No significant difference in the cumulative amount of leakage was found between the three experimental groups at all observation times. The lateral condensation of cold gutta-percha can guarantee a similar seal of canal fillings as can be achieved by using thermal methods, in the round canals.

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The Effectiveness Differences Between Watermelon (Citrullus lanatus) Extract 100% and Carbamide Peroxide Gel 10% in Tooth Whitening (ex vivo)

Journal of Indonesian Dental Association ◽

10.32793/jida.v3i1.429 ◽

2020 ◽

Vol 3 (1) ◽

pp. 31

Author(s):

Any Setyawati ◽

Syifa Nabila Farah Fauziah Nur

Keyword(s):

Malic Acid ◽

Ex Vivo ◽

Citrullus Lanatus ◽

Black Tea ◽

Positive Control ◽

Negative Control ◽

Carbamide Peroxide ◽

Extrinsic Factors ◽

Significant Difference ◽

Group 2

Introduction: Discoloration can be caused by intrinsic or extrinsic factors. One of the discoloration treatments is teeth whitening. Teeth whitening process usually uses chemicals such as hydrogen peroxide or carbamide peroxide which can cause side effects, namely gingival irritation. Previous research has found that malic acid in strawberries can whiten teeth. Watermelons contain greater malic acid than strawberries. Objective: To analyze the effectiveness of 100% watermelon (Citrullus lanatus) extract on teeth whitening. Methods: The study was a laboratory experimental study with a total of 15 anterior post-extraction teeth which were discolored using black tea, divided into 3 groups. Group 1 was immersed in 100% watermelon extract, group 2 was immersed in 10% carbamide peroxide as positive control and group 3 was immersed in sterile aquades as negative control, for 56 hours, measured using a shade guide and spectrophotometer. Data were analyzed using one way Anova. Results: The 100% watermelon extract was effective for teeth whitening. There was a significant difference between 100% watermelon extract compared to negative control (p < 0.05). However, there was also a significant difference between 100% watermelon extract, and 10% carbamide peroxide gel (p = 0.003). Conclusion: The watermelon extract has the ability as teeth whitening agent. However, further study is still needed to explore this result and determine the proper concentration for teeth whitening.

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Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

Revista de Biología Tropical ◽

10.15517/rbt.v66i4.33140 ◽

2018 ◽

Vol 66 (4) ◽

pp. 1519

Author(s):

Mailen Ortega Cuadros ◽

Adriana Patricia Tofiño Rivera ◽

Luciano Jose Merini ◽

Maria Cecilia Martinez Pabon

Keyword(s):

Antimicrobial Activity ◽

Dental Caries ◽

Essential Oil ◽

Streptococcus Mutans ◽

Human Fibroblasts ◽

Positive Control ◽

Negative Control ◽

Cymbopogon Citratus ◽

Significant Difference ◽

Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

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Alternative sources of potassium for maize cultivation

Ciência e Agrotecnologia ◽

10.1590/1413-7054201943023619 ◽

2019 ◽

Vol 43 ◽

Author(s):

Paulo Fernandes Boldrin ◽

Hugo Ferreira Souto ◽

Lenise Selaysim Salles ◽

Antonio Eduardo Furtini Neto

Keyword(s):

Plant Height ◽

Clayey Soil ◽

Poultry Manure ◽

Dry Mass ◽

Positive Control ◽

Negative Control ◽

Cattle Manure ◽

Stem Diameter ◽

Chicken Manure ◽

Alternative Sources

ABSTRACT Brazil is the largest importer of potassium (K) fertilizers in the world, importing about 95% of all the potassium consumed by its agriculture. Thus, new sources and technologies to increase K availability have been studied. This study aimed to evaluate the agronomic viability of the use of calcined glauconite and phonolite blended and granulated with two organic materials on maize growth in pots, using two soils of contrasting texture in a controlled environment. Were evaluated eight sources of potassium: phonolite; phonolite + poultry manure; phonolite + cattle manure; calcined glauconite; calcined glauconite + poultry manure; calcined glauconite + cattle manure; negative control (no K); and positive control (K - KCl), and two soils of contrasting texture. The experiment was carried out for 38 d. The following parameters were analyzed: plant height, stem diameter, shoot dry mass, leaf number, leaf contents of nitrogen (N), K and phosphorus (P), and the SPAD chlorophyll meter. In the sandy soil, the source consisting of phonolite + chicken manure was similar to KCl application regarding plant height, stem diameter and shoot dry mass. As for the clayey soil, when both rocks were blended with the organic wastes, they were similar to KCl for shoot dry mass. The use of pure granulated rocks, calcined glauconite and phonolite without blending with the organic residues is not efficient to provide available K to maize plants.

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A Digital Microscopic Inspection of Dentinal Defects after Using Endodontic Retreatment Files

International Journal of Dentistry ◽

10.1155/2021/6661387 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Ammar AbuMostafa ◽

Hala Almoqayyad ◽

Al-Omari Mohammad

Keyword(s):

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Filling Material ◽

Root Canals ◽

Root Canal Retreatment ◽

Significant Difference ◽

Digital Microscope ◽

Microscopic Inspection

Aim. The study aimed at evaluating the incidence of dentinal defects after root canal retreatment with ProTaper Universal retreatment (PTUR) and XP-endo Shaper and Finisher R (XP). Materials and Methods. Sixty extracted single-rooted human premolars were selected and divided into 4 groups of 15 teeth each. In the negative control group, the teeth were left unprepared. In the positive control group, the teeth were prepared with ProTaper Next and obturated with no further retreatment. In the PTUR and XP groups, the teeth were prepared and obturated followed by removal of the filling material at body temperature using PTUR and XP instruments, respectively. The roots were then sectioned at 3, 6, and 9 mm from the apex and observed under a digital microscope to detect defects. Results. PTUR group showed significantly higher ( p value <0.05) incidence of defects than the other groups. Comparison of no defects versus defects between groups in different areas of root canals demonstrated significant difference among the groups in the apical and cervical regions. Conclusion. Within the limitations of the present study, PTUR files created significantly more dentinal defects than XP files, with most of those defects at the cervical and apical areas of the root canals.

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In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2021/v23i930256 ◽

2021 ◽

pp. 12-20

Author(s):

Udeme O. Georgewill ◽

Festus Azibanigha Joseph ◽

Elias Adikwu

Keyword(s):

Plasmodium Berghei ◽

Blood Cells ◽

Hematological Parameters ◽

White Blood Cells ◽

Positive Control ◽

Negative Control ◽

Antiplasmodial Activity ◽

Significant Difference ◽

Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite. NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

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Pengaruh Induksi Resistensi oleh Khamir Candida tropicalis terhadap Pertumbuhan Tanaman Cabai Terinfeksi Colletotrichum acutatum

Agrikultura ◽

10.24198/agrikultura.v30i1.22699 ◽

2019 ◽

Vol 30 (1) ◽

pp. 17

Author(s):

Sri Hartati ◽

Linda Tarina ◽

Endah Yulia ◽

Luciana Djaya

Keyword(s):

Plant Height ◽

Induced Resistance ◽

Candida Tropicalis ◽

Yield Loss ◽

Biocontrol Agent ◽

Dry Weight ◽

Positive Control ◽

Negative Control ◽

Leaf Number ◽

Colletotrichum Acutatum

ABSTRACTEffect of induced resistance by Candida tropicalis on the growth of chili plants infected by Colletotrichum acutatumColletotrichum acutatum is a major pathogen of chili that causes a big yield loss. Candida tropicalis has been reported as a biocontrol agent antagonistic to C. acutatum. The research was objected tofind out the effect of induced resistance by C. tropicalis on the growth of chili plants infected by C. acutatum. The application of the yeast was carried out by seed dipping and seedling spraying methods. As treatments, C. acutatum, the pathogen was inoculated at different time, i.e. A = inoculation at 3 days after the induction (dai) by C. tropicalis, B = inoculation at 7 dai, C = inoculation at 10 dai, D = positive control on inoculation at 3 dai, E = positive control on inoculation at 7 dai, F = positive control on inoculation at 10 dai, G = negative control with no induction nor inoculation. The results showed that the induction by C. tropicalis was able to increase the plant height, leaf number, and dry weight. The highest increase of plant height of 30.72% was caused by induction of C. tropicalis inoculated at 7 dai. Induction of C. tropicalis inoculated at 3 dai increased the leaf number by 49.47%, and the plant dry weight by 50%. C. tropicalis has a potency to be developed as plant growth inducer.Keywords: Antagonist, Inoculation, Fungi, YeastABSTRAKJamur Colletotrichum acutatum merupakan patogen penyebab kehilangan hasil panen yang cukup besar pada tanaman cabai. Salah satu mikrob antagonis jamur ini yang telah dilaporkan adalah khamir Candida tropicalis. Penelitian ini bertujuan untuk mengetahui pengaruh induksi resistensioleh C. tropicalis terhadap pertumbuhan tanaman cabai terinfeksi C. acutatum. Penelitian dilaksanakan dengan menggunakan teknik perendaman benih dan penyiraman bibit tanaman cabaimenggunakan suspensi C. tropicalis dengan waktu inokulasi patogen C. acutatum yang berbeda. Perlakuan tersebut adalah A = Inokulasi C. acutatum 3 hari setelah perlakuan induksi C. tropicalis (hsp), B = Inokulasi C. acutatum 7 hsp, C = Inokulasi C. acutatum 10 hsp, D = Kontrol (+) dengan inokulasi C. acutatum 3 hsp, E = Kontrol (+) dengan inokulasi C. acutatum 7 hsp, F = Kontrol (+) dengan inokulasi C. acutatum 10 hsp, G = Kontrol (-) tanpa induksi dan inokulasi. Hasil penelitianmenunjukkan bahwa perlakuan induksi resistensi oleh khamir C. tropicalis mampu meningkatkan tinggi, jumlah daun, dan bobot kering tanaman cabai. Kemampuan meningkatkan tinggi tanamancabai tertinggi terjadi pada perlakuan C. tropicalis dengan waktu inokulasi 7 hsp yaitu sebesar 30,72%. Perlakuan khamir C. tropicalis dengan inokulasi patogen 3 hsp mampu meningkatkan jumlah daun tanaman cabai sebesar 49,47%. Perlakuan C. tropicalis dengan waktu inokulasi 3 hsp mampu meningkatkan bobot kering tanaman sebesar 50%. Khamir C. tropicalis berpotensi untuk dikembangkan sebagai agens pemacu pertumbuhan tanaman.Kata Kunci: Antagonis, Inokulasi, Jamur, Patogen

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