scholarly journals Prevalence of anti-leptospiral antibodies and frequency distribution of Leptospira serovars in small ruminants in enzootic South Peninsular India

2021 ◽  
pp. 2023-2030
Author(s):  
Vinayagamurthy Balamurugan ◽  
Anusha Alamuri ◽  
Kirubakaran Vinod Kumar ◽  
Bibitha Varghese ◽  
Gurrappanaidu Govindaraj ◽  
...  
Keyword(s):  
Frequency Distribution ◽  
Small Ruminants ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Chi Square ◽  
Peninsular India ◽  
Administrative Division ◽  
Chi Squared ◽  
Leptospira Serovars ◽  
Apparently Healthy

Background and Aim: For understanding the epidemiology of leptospirosis, the confined abundance of several species of pathogenic leptospires and knowledge on the serovar(s) prevalent in the reservoir and carrier hosts may be a useful indicator of transmission to incidental/accidental hosts in a geographical niche. The present study was carried out to ascertain the frequency distribution of Leptospira serovars and the prevalence of anti-leptospiral antibodies in small ruminants (sheep and goats) in the epidemiological units (villages) in the coastal districts of enzootic regions in South Peninsular India. Materials and Methods: A total of 1167 serum samples (sheep n=299 and goats n=868) from apparently healthy animals, randomly collected from various epidemiological units were tested in microscopic agglutination test (MAT) using 18 reference Leptospira serovars antigens. Results: The overall seroprevalence of 40% (at 95% confidence intervals [CI]: 36.82-42.43) in small ruminants (44% [95% CI: 40.49-52.26] in sheep and 38% [95% CI: 34.96-41.41] in goats) was observed with the predominance of Icterohaemorrhagiae, Javanica, Australis, Hurstbridge, and Pyrogenes serogroup anti-leptospiral antibodies in the studied region. The Chi-squared test revealed that the presence of anti-leptospiral antibodies is significantly not independent (associated) across the administrative division (Chi-square=105.80, p<0.05) as well as for sheep (Chi-square=34.67, p<0.01) and goats (Chi-square=68.78, p<0.01). Among seropositive samples (n=462 reactors), the MAT was positive for more than one serovar in 73% of sheep (95/131) and 53% of goats (177/331), representing an overall 59% cross-reactive prevalence in small ruminants. The determined frequency distribution (varied among small ruminants) of the employed serovars representing major reactive serogroup was Icterohaemorrhagiae (29.87), Javanica (20.78), Australis (20.35), Hurstbridge (16.23), Pyrogenes (15.8), Djasmin (15.58), Bataviae (15.37), Autumnalis (14.5), Canicola (14.5), Hebdomadis (14.07), Shermani (13.64), Panama (13.42), Sejroe (12.77), etc. Conclusion: This study indicates alarmingly high seroprevalence of leptospirosis in small ruminants with existing endemicity in the studied region in South Peninsular India. Further, these prevalent serovars in the administrative division may be of use in the reference panels of antigens in the MAT in both humans and animal disease diagnostic laboratories for effective and timely diagnosis of leptospirosis and to combat the challenges in public health.

scholarly journals Serological Investigation of Peste Des Petits Ruminants in East Shewa and Arsi Zones, Oromia Region, Ethiopia

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Getachew Gari ◽  
Biressaw Serda ◽  
Dejene Negesa ◽  
Fethu Lemma ◽  
Hagos Asgedom
Keyword(s):  
Significant Variation ◽  
Small Ruminants ◽  
Control Measures ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Seroprevalence ◽  
Serum Samples ◽  
Male And Female ◽  
Significant Difference ◽  
Important Disease

Peste des petits ruminant (PPR) is an economically important disease of small ruminants with a rapidly expanding geographical distribution. There are fragmented reports to the occurrence and distribution of the disease in Ethiopia. A total of 700 serum samples were collected from goats and sheep to detect the presence of antibody against PPR virus using Competitive Enzyme-Linked Immunosorbent Assay (C-ELISA). An overall PPR seropositivity was reported to be 48.43% in the area. There is no statistically significant difference in the seroprevalence of the disease between sheep and goats (50.85% and 46.68%), respectively. However, there was statistically significant variation (P<0.05) in the seroprevalence of the disease in young (33.9%) and adult (55.8%) age categories. The seroprevalence in male and female was 42.07% and 50.09%, respectively, where the variation was statistically not significant (P>0.05). High seroprevalence of Peste des petites ruminants in the study area indicated the virus circulation and endemicity of the disease. The disease causes substantial economic losses by affecting the livelihood of the farmers. Therefore, control measures should be put in place to minimize the loss associated with the disease.

scholarly journals Pola SegregasiKarakter KetahananTanaman Kedelai(Glycine max [L]. Merrill)Terhadap Infeksi Soybean Mosaic Virus Populasi F2 Keturunan Taichung X Tanggamus

2017 ◽  
Vol 15 (1) ◽  
Author(s):  
Nidya Wanda ◽  
Maimun Barmawi ◽  
Hasriadi Mat Akin ◽  
Nyimas Sa’diyah
Keyword(s):  
Frequency Distribution ◽  
Soybean Mosaic Virus ◽  
Single Gene ◽  
Disease Spread ◽  
Soybean Plant ◽  
Chi Square ◽  
Chi Squared ◽  
Chi Square Test ◽  
Severity Of The Disease ◽  
F2 Generation

This study aims to determine the pattern of segregation character soybean plant resistance to infection SMV and other agronomic characters as well as many genes that play a role in the inheritance of these traits in the F2 populations from crosses Taichung x Tanggamus follow of Mendel ratio or a modification of expectations and knowing the number of crosses. This research was conducted at the Laboratory LapanganTerpadu Faculty of Agriculture, University of Lampung from September 2013 until January 2014, and then continued in the laboratory observations Seed and Plant Breeding, University of Lampung. Propagation of virus carried in Kampung Baru, Bandar Lampung. This research uses experimental design without repetition with a single treatment design nested structured data is analyzed using the chi-square test for the suitability of the normal distribution and chi-squared test to test the of Mendel ratio and modifications . The results of this study indicate the character frequency distribution of plant height, grain weight per plant soybeans F2 generation from crosses Taichung x Tanggamus normal spread, while the frequency distribution for character flowering age, harvesting, healthy seed number per plant, number of productive branches, number of pods per plants and severity of disease spread is not normal. Estimates of the number of genes that control the character of harvesting is controlled by two genes are recessive epistasis duplicate the ratio 9: 7, days to flowering follow the ratio 1: 2: 1 which is controlled by a single gene that reacts not perfect, healthy character of the number of seeds per plant followed the ratio 3: 1 and for the total character of pods per plant, number of productive branches as well as the severity of the disease follows the ratio of 13: 3. the character dikendalikanolehdua genes dominant-recessive epistasis react. There are 21 numbers belonging expectations resistant to SMV. Keywords: Soybean, segregation, resistance characteristics, and SMV.

scholarly journals Anaplasma spp. in dairy ruminants in Jordan: high individual and herd-level seroprevalence and association with abortions

2019 ◽  
Vol 31 (3) ◽  
pp. 481-484 ◽  
Author(s):  
Mohammad M. Obaidat ◽  
Alaa E. Bani Salman
Keyword(s):  
Small Ruminants ◽  
Dairy Farms ◽  
Fisher Exact Test ◽  
High Seroprevalence ◽  
Exact Test ◽  
Sheep And Goats ◽  
Associated Risk Factors ◽  
The Individual ◽  
Apparently Healthy

We estimated the seroprevalence of, and associated risk factors for, anaplasmosis in dairy cows, sheep, and goats in all regions of Jordan. Apparently healthy animals from 135 farms (31 cow, 68 sheep, and 36 goat farms) were tested for Anaplasma spp. antibodies by a competitive ELISA. At the farm level, 36%, 94%, and 94% of cow, sheep, and goat farms, respectively, were seropositive for Anaplasma spp. At the individual animal level, 22%, 89%, and 82% of the tested cows, sheep, and goats, respectively, were seropositive, which demonstrates widespread and high seroprevalence of Anaplasma spp. in dairy farms in Jordan. Seroprevalence did not vary between regions. Using the Fisher exact test, there was a significant association between high seroprevalence and recent abortion in small ruminants. There is an urgent need for further investigation of this disease, and the potential role of Anaplasma spp. in abortions, on dairy farms in Jordan.

PENERAPAN CHAID DENGAN PENDEKATAN SMOTE PADA KEMATIAN BALITA DI KAWASAN TIMUR INDONESIA TAHUN 2017

2020 ◽  
Vol 2019 (1) ◽  
pp. 357-367
Author(s):  
Isti Samrotul Hidayati ◽  
I Made Arcana
Keyword(s):  
Sampling Technique ◽  
Chi Square ◽  
Interaction Detection ◽  
Chi Squared

Metode Chi-squared Automatic Interaction Detection (CHAID) merupakan metode segmentasi berdasarkan hubungan variabel respon dan penjelas menggunakan uji chi-square, yang dalam penerapannya perlu memperhatikan keseimbangan data untuk meminimalkan kesalahan dalam klasifikasi. Salah satu pendekatan yang dapat digunakan pada data yang tidak seimbang adalah metode Synthetic Minority Over-sampling Technique (SMOTE). Dalam penelitian ini, metode CHAID dengan pendekatan SMOTE diterapkan pada Angka Kematian Balita (AKBa) di Kawasan Timur Indonesia (KTI). Tujuannya adalah untuk mengetahui variabel-variabel yang mencirikan kematian balita berdasarkan metode analisis CHAID yang diterapkan dan membandingkannya dengan pendekatan SMOTE. Hasil perbandingan menunjukkan bahwa pendekatan SMOTE lebih baik digunakan dengan nilai sensitivitas sebesar 48,3% dan nilai presisi sebesar 75,9%. Variabel yang signifikan mencirikan kematian balita di KTI adalah berat badan saat lahir, jenis kelahiran, status bekerja ibu dan kekayaan rumah tangga, dengan karakteristik utama adalah balita yang memiliki berat badan lahir rendah dan terlahir kembar.

scholarly journals Molecular Detection and Phylogeny of Anaplasmaphagocytophilum and Related Variants in Small Ruminants from Turkey

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 814
Author(s):  
Münir Aktaş ◽  
Sezayi Özübek ◽  
Mehmet Can Uluçeşme
Keyword(s):  
16S Rrna ◽  
Small Ruminants ◽  
Rrna Gene ◽  
Infection Rates ◽  
Sheep And Goats ◽  
Significant Difference ◽  
Pcr Rflp ◽  
First Time ◽  
Japan And China ◽  
Apparently Healthy

Anaplasma phagocytophilum causes tick-borne fever in small ruminants. Recently, novel Anaplasma variants related to A. phagocytophilum have been reported in ruminants from Tunisia, Italy, South Korea, Japan, and China. Based on 16S rRNA and groEL genes and sequencing, we screened the frequency of A. phagocytophilum and related variants in 433 apparently healthy small ruminants in Turkey. Anaplasma spp. overall infection rates were 27.9% (121/433 analyzed samples). The frequency of A. phagocytophilum and A. phagocytophilum-like 1 infections was 1.4% and 26.5%, respectively. No A. phagocytophilum-like 2 was detected in the tested animals. The prevalence of Anaplasma spp. was comparable in species, and no significant difference was detected between sheep and goats, whereas the prevalence significantly increased with tick infestation. Sequencing confirmed PCR-RFLP data and showed the presence of A. phagocytophilum and A. phagocytophilum-like-1 variant in the sampled animals. Phylogeny-based on 16S rRNA gene revealed the A. phagocytophilum-like 1 in a separate clade together with the previous isolates detected in small ruminants and ticks. In this work, A. phagocytophilum-like 1 has been detected for the first time in sheep and goats from Turkey. This finding revealed that the variant should be considered in the diagnosis of caprine and ovine anaplasmosis.

scholarly journals Seropositivity to canine tick-borne pathogens in a population of sick dogs in Italy

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jairo Alfonso Mendoza-Roldan ◽  
Giovanni Benelli ◽  
Marcos Antonio Bezerra-Santos ◽  
Viet-Linh Nguyen ◽  
Giuseppe Conte ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Serological Test ◽  
Control Strategies ◽  
Central Italy ◽  
Rickettsia Conorii ◽  
High Seroprevalence ◽  
The South ◽  
Serum Samples ◽  
Serological Tests ◽  
Distribution Maps

Abstract Background Canine vector-borne diseases (CVBDs) associated to ticks are among the most important health issues affecting dogs. In Italy, Ehrlichia canis, Anaplasma spp., Rickettsia conorii and Borrelia burgdorferi (s.l.) have been studied in both healthy canine populations and those clinically ill with suspected CVBDs. However, little information is currently available on the overall prevalence and distribution of these pathogens in the country. The aim of this study was to assess the prevalence and distribution of tick-borne pathogens (TBPs) in clinically suspect dogs from three Italian macro areas during a 15-year period (2006–2020). Methods A large dataset (n = 21,992) of serological test results for selected TBPs in three macro areas in Italy was analysed using a Chi-square test to evaluate the associations between the categorical factors (i.e. macro area, region, year, sex and age) and a standard logistic regression model (significance set at P = 0.05). Serological data were presented as annual and cumulative prevalence, and distribution maps of cumulative positive cases for TBPs were generated. Results Of the tested serum samples, 86.9% originated from northern (43.9%) and central (43%) Italy. The majority of the tests was requested for the diagnosis of E. canis (47%; n = 10,334), followed by Rickettsia spp. (35.1%; n = 7725), B. burgdorferi (s.l.) (11.6%; n = 2560) and Anaplasma spp. (6.2%; n = 1373). The highest serological exposure was recorded for B. burgdorferi (s.l.) (83.5%), followed by Rickettsia spp. (64.9%), Anaplasma spp. (39.8%) and E. canis (28.7%). The highest number of cumulative cases of Borrelia burgdorferi (s.l.) was recorded in samples from Tuscany, central Italy. Rickettsia spp. was more prevalent in the south and on the islands, particularly in dogs on Sicily older than 6 years, whereas Anaplasma spp. was more prevalent in the north and E. canis more prevalent in the south and on the islands. Conclusions The results of this study highlight the high seroprevalence and wide distribution of the four TBPs in dogs with clinically suspected CVBDs from the studied regions of Italy. The very high seroprevalence of B. burgdorferi (s.l.) exemplifies a limitation of this study, given the use of clinically suspect dogs and the possibility of cross-reactions when using serological tests. The present research provides updated and illustrative information on the seroprevalence and distribution of four key TBPs, and advocates for integrative control strategies for their prevention. Grapic abstract

scholarly journals Leptospirosis in Ruminants in Yogyakarta, Indonesia: A Serological Survey with Mixed Methods to Identify Risk Factors

2021 ◽  
Vol 6 (2) ◽  
pp. 84
Author(s):  
Dyah Ayu Widiasih ◽  
Johanna Frida Lindahl ◽  
Wayan T. Artama ◽  
Adi Heru Sutomo ◽  
Pande Made Kutanegara ◽  
...  
Keyword(s):  
Risk Factor ◽  
Management Practices ◽  
Small Ruminants ◽  
Case Fatality ◽  
Open Water ◽  
Serum Samples ◽  
Public Health Importance ◽  
Group Discussions ◽  
The Individual ◽  
And Behavior

Leptospirosis is a zoonotic disease occurring worldwide with reproductive symptoms and production losses in livestock, while humans can suffer fatal renal failure. In Yogyakarta Special Province, Indonesia, there have been several outbreaks with high case fatality, demonstrating the public health importance, but there is limited understanding of the epidemiology. This study used an EcoHealth approach to ensure transdisciplinarity and community participation. Seroprevalence of Leptospira in animals was studied between October 2011 and May 2013 in 15 villages. Serum samples from 1404 cattle and 60 small ruminants were screened by a Microscopic Agglutination Test (MAT), first in pools, and then the individual positive samples were identified. Focus group discussions including farmers, village officials, and official stakeholders were used to explore knowledge and behavior of zoonotic diseases, particularly leptospirosis. Two small ruminants were seropositive for Leptospira icterohemorrhagiae. From the cattle, 3.7% were seropositive, and the most common serovars were Leptospira hardjo, followed by L. icterohemorrhagiae. Out of all farms, 5.6% had at least one positive cattle. Risk factor analyses showed that the risk of the farm being seropositive increased if the farmer used water from an open source, or if farming was not the main occupation. This study showed the presence of Leptospira spp. in ruminants in Yogyakarta and identified use of open water as a risk factor for the livestock. We also observed that the knowledge related to leptospirosis was low, and risky farm management practices were commonly employed.

scholarly journals Seroepidemiological study of toxoplasmosis in women referred to a pre-marriage counseling center in Alborz Province, Iran

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Melica Shahighi ◽  
Aliehsan Heidari ◽  
Hossein Keshavarz ◽  
Amir Bairami ◽  
Saeedeh Shojaee ◽  
...  
Keyword(s):  
Congenital Toxoplasmosis ◽  
Pcr Analysis ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Serum Samples ◽  
Chi Square ◽  
Igg Avidity ◽  
Two Samples ◽  
Chi Square Test ◽  
Associated Risk Factors

Abstract Objectives The aim of the current study was to assess prevalence of Toxoplasma infection and its associated risk factors in women of childbearing-age in central Iran. Results Of 400 serum samples assessed for anti-T. gondii antibodies, 81 (20.25%) samples were positive for anti-T. gondii antibodies, including 74 positive samples (91.3%) for anti-T. gondii IgG and seven positive samples (8.7%) for IgG and IgM. Of seven IgG and IgM positive samples, five and two samples were high and low in IgG avidity, respectively. Based on PCR analysis, Toxoplasma infection was detected in one sample with anti-T. gondii IgM and low IgG avidity. The Chi-square test showed significant correlations of T. gondii seropositivity with history of undercooked meat consumption and contacts with cats (p < 0.05). In the present study, 79.75% of the participants were negative for IgG against T. gondii infection. Furthermore, recently acquired Toxoplasma infection was found using IgG avidity and PCR assays among women of childbearing-age in the study area, which would increase the risk of their fetus becoming infected. Educational program and antenatal screening of childbearing-age women for T. gondii infection may be important primary prevention strategies and help reduce the risk of congenital toxoplasmosis in this population.

scholarly journals Review of Peste des Petits Ruminants Occurrence and Spread in Tanzania

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1698
Author(s):  
Daniel Pius Mdetele ◽  
Erick Komba ◽  
Misago Dimson Seth ◽  
Gerald Misinzo ◽  
Richard Kock ◽  
...  
Keyword(s):  
Economic Impact ◽  
Disease Surveillance ◽  
Grey Literature ◽  
Small Ruminants ◽  
Peste Des Petits Ruminants ◽  
Serum Samples ◽  
Eradication Programme ◽  
Sheep And Goats ◽  
Ruminant Species ◽  
Socio Economic Impact

Peste des petits ruminants (PPR) is an important transboundary animal disease of domestic small ruminants, camels, and wild artiodactyls. The disease has significant socio-economic impact on communities that depend on livestock for their livelihood and is a threat to endangered susceptible wild species. The aim of this review was to describe the introduction of PPR to Tanzania and its subsequent spread to different parts of the country. On-line databases were searched for peer-reviewed and grey literature, formal and informal reports were obtained from Tanzanian Zonal Veterinary Investigation Centres and Laboratories, and Veterinary Officers involved with PPR surveillance were contacted. PPR virus (PPRV) was confirmed in northern Tanzania in 2008, although serological data from samples collected in the region in 1998 and 2004, and evidence that the virus was already circulating in Uganda in 2003, suggests that PPRV might have been present earlier than this. It is likely that the virus which became established in Tanzania was introduced from Kenya between 2006–7 through the cross-border movement of small ruminants for trade or grazing resources, and then spread to eastern, central, and southern Tanzania from 2008 to 2010 through movement of small ruminants by pastoralists and traders. There was no evidence of PPRV sero-conversion in wildlife based on sera collected up to 2012, suggesting that they did not play a vectoring or bridging role in the establishment of PPRV in Tanzania. PPRV lineages II, III and IV have been detected, indicating that there have been several virus introductions. PPRV is now considered to be endemic in sheep and goats in Tanzania, but there has been no evidence of PPR clinical disease in wildlife species in Tanzania, although serum samples collected in 2014 from several wild ruminant species were PPRV sero-positive. Similarly, no PPR disease has been observed in cattle and camels. In these atypical hosts, serological evidence indicates exposure to PPRV infection, most likely through spillover from infected sheep and goats. Some of the challenges for PPRV eradication in Tanzania include movements of small ruminants, including transboundary movements, and the capacity of veterinary services for disease surveillance and vaccination. Using wildlife and atypical domestic hosts for PPR surveillance is a useful indicator of endemism and the ongoing circulation of PPRV in livestock, especially during the implementation of vaccination to control or eliminate the disease in sheep and goats. PPR disease has a major socio-economic impact in Tanzania, which justifies the investment in a comprehensive PPRV eradication programme.

Detection of Protein Changes in Serum of Workers following Inhalation Exposure to Toluene Diisocyanate Vapors

1992 ◽  
Vol 8 (6) ◽  
pp. 407-413 ◽  
Author(s):  
Adam B. Czuppon ◽  
Boleslaw Marczynski ◽  
Xaver Baur
Keyword(s):  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Inhalation Exposure ◽  
Staining Intensity ◽  
Peak Height ◽  
Toluene Diisocyanate ◽  
Serum Samples ◽  
Chi Square ◽  
Challenge Tests

Serum samples of 10 workers undergoing occupational type inhalative challenge tests by toluene diisocyanate (TDI) were investigated by anion-exchange fast-protein-liquid-chromatography (FPLC) and polyacrylamide-gel electrophoresis (PAGE-SDS). Their serum chromatography profiles were compared to those of 20 unexposed individuals. The peak height of the first prealbumin peak in sera of workers after inhalative challenge tests was significantly different (p > 0, 01 Chi-square test) compared to that obtained before exposure and to that of unexposed subjects. In addition, qualitative changes of these peaks were also noted in sera of workers exposed to TDI. In the cases of exposed individuals, that peak was more diffuse with some shoulders and less symmetric in appearance. Similarly, PAGE-SDS of the serum proteins, followed by silver nitrate staining, revealed a different banding pattern after in vivo TDI exposure. One of the serum components at approximately 15 kD showed an increase of staining intensity after exposure (n = 10), compared to unexposed subjects or to patients before exposure. This serum fraction has not yet been identified. The results here demonstrate that it is possible to detect changes of serum proteins in TDI-exposed individuals within a relatively short analysis time. This could be useful for biological monitoring of exposure, since no method for such is yet available.

Sign in / Sign up

Export Citation Format

Share Document