scholarly journals Performance and Small Intestine Morphology and Ultrastructure of Male Broilers Injected in Ovo with Bioactive Substances

2017 ◽  
Vol 17 (1) ◽  
pp. 179-195 ◽  
Author(s):  
Joanna Bogucka ◽  
Agata Dankowiakowska ◽  
Gabriela Elminowska-Wenda ◽  
Adrianna Sobolewska ◽  
Jan Jankowski ◽  
...  
Keyword(s):  
Small Intestine ◽  
Broiler Chickens ◽  
Histological Analysis ◽  
Physiological Saline ◽  
Goblet Cells ◽  
Control Group ◽  
In Ovo ◽  
Bioactive Substances ◽  
Broiler Performance ◽  
Intestinal Villi

AbstractThe objective of the present study was to determine the effect of prebiotic and synbiotic preparations injected in ovo on day 12 of embryogenesis on both development of intestinal villi and the number of neutral goblet cells in the small intestine of male broiler chickens on day 35 of rearing. Eggs containing live embryos were randomly separated into five experimental groups (1800 eggs per group), and treated with different bioactive compounds by in ovo injection. The control group (C) was injected with physiological saline. The prebiotic groups (PI and PB) were injected with a solution containing 1.76 mg of inulin or with a solution containing 0.528 mg of Bi2tos. The injection solution for both synbiotic groups (SI and SB) consisted of 1.76 mg Inulin + 1000 CFU of L. lactis spp. lactis 2955 (SL1) or 0.528 mg Bi2tos + 1000 CFU of L. lactis spp. cremoris 477 (SC1). Samples for histological analysis were taken from the three segments of the small intestine: the duodenum, jejunum and ileum. Broiler performance increased in the prebiotic group injected with Bi2tos when compared to both the control group and the prebiotic group injected with inulin. In relation to other groups, in the duodenum and ileum the highest intestinal villi were observed in chickens with the lowest body weight, i.e. groups C and PI. The smaller surface area of villi was found in the jejunum and ileum in group SB. As far as the jejunum and ileum are concerned, a significantly higher number of goblet cells was noted in groups PB and SB.

Performance of broilers fed with different levels of sunflowermeal supplemented with or without enzymes

2016 ◽  
Author(s):  
Ergin Ozturk
Keyword(s):  
Small Intestine ◽  
Broiler Chickens ◽  
Positive Impact ◽  
Feed Consumption ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Broiler Performance ◽  
Treatment Groups ◽  
Randomized Design

In this study, it was aimed to determine the performances and some carcass and small intestine properties of broiler chickens fed diets in which sunflower meal (SFM) replaced soybean meal (SBM) and supplemented or not with multi-enzyme. With this aim, a total of 450 1-day-old age mixed-sex Ross 308 broiler chicks were used in a completely randomized design, with five treatments and three replicates of thirty birds each. The experimental diets consisted of a control group and two levels of SFM with or without enzyme replacing 50 or 100% of SBM protein, respectively. Hence, treatment groups were: control (100SBM), 50SFM, 100SFM, 50SFME, and 100SFME). Feed and fresh water was given freely. At the end of the experiment (42 days of age), 100SBM broilers had higher body weight and cold carcass weight than all other groups (P is less than 0.05) and had lower feed conversion ratio (FCR) compared to 50SFME broilers (P less than 0.05). Feed consumption, weights of hearth, liver, gizzard, abdominal fat and small intestine and length of small intestine were not found different among the groups (P is less than0.05). Our observations demostrated that addition of the 0.05% multi-enzyme mixture into SFM diets of broiler chickens had not positive impact on the availability of SFM and broiler performance

scholarly journals Effects of in ovo Injection of Astragalus Polysaccharide on the Intestinal Development and Mucosal Immunity in Broiler Chickens

2021 ◽  
Vol 8 ◽  
Author(s):  
Shu-bao Yang ◽  
Yan-jun Qin ◽  
Xin Ma ◽  
Wei-min Luan ◽  
Peng Sun ◽  
...  
Keyword(s):  
Body Weight ◽  
Mucosal Immunity ◽  
Broiler Chickens ◽  
Early Stage ◽  
Physiological Saline ◽  
The Body ◽  
Control Group ◽  
Intestinal Development ◽  
In Ovo ◽  
Time Points

The purpose of this study was to examine the effects of in ovo injection of Astragalus polysaccharide (APS) on hatchability, body weight (BW), intestinal histomorphology, the number of IgA+ cells and sIgA content in intestine, and the expression of intestinal immune-related genes in broiler chickens. On day 18 of the incubation, a total of 960 live embryo eggs were weighed and randomly divided into 4 treatment groups: a control group and three APS groups. The eggs in the control group were injected with 0.5 mL physiological saline. The eggs in the APS groups were injected with 3 different amounts of APS in 0.5 mL physiological saline: 1 mg (APSL), 2 mg (APSM) and 4 mg (APSH). The solution was injected into the amnion of each egg. The results showed that in ovo injection of APS did not affect the hatchability but increased the body weight of the 14 d and 21 d chickens, with a significant increase observed in the APSM group (P < 0.05). At most time points, the villus height (VH) was increased (P < 0.05) and the crypt depth (CD) was decreased (P < 0.05) in the small intestine of the broilers, with higher VH/CD ratios in the APSL and APSM groups compared with the control group. The number of IgA+ cells in the mucosa and the secretory immunoglobulin A (sIgA) levels in the intestinal washings were higher in the APSM and APSH groups than in the APSL and control groups. The gene expression levels of interleukin (IL)-2, interleukin (IL)-4, interferon gamma (IFN-γ), and Toll-like receptor (TLR)-4 were significantly enhanced by APS stimulation at most time points (P < 0.05). These results indicated that in ovo injection of APS has the potential of promoting intestinal development and enhancing intestinal mucosal immunity of broiler chickens in the early stage after hatching.

scholarly journals Calcium Carbonate Nanoparticles—Toxicity and Effect of In Ovo Inoculation on Chicken Embryo Development, Broiler Performance and Bone Status

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 932
Author(s):  
Arkadiusz Matuszewski ◽  
Monika Łukasiewicz ◽  
Jan Niemiec ◽  
Maciej Kamaszewski ◽  
Sławomir Jaworski ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Embryo Development ◽  
Bone Quality ◽  
Chicken Embryo ◽  
Broiler Chickens ◽  
Selection Procedure ◽  
High Specificity ◽  
Control Group ◽  
In Ovo

The use of intensive selection procedure in modern broiler chicken lines has led to the development of several skeletal disorders in broiler chickens. Therefore, current research is focused on methods to improve the bone quality in birds. In ovo technology, using nanoparticles with a high specificity to bones, is a potential approach. The present study aimed to evaluate the effect of in ovo inoculation (IOI) of calcium carbonate nanoparticles (CCN) on chicken embryo development, health status, bone characteristics, and on broiler production results and bone quality. After assessing in vitro cell viability, the IOI procedure was performed with an injection of 500 μg/mL CCN. The control group was not inoculated with CCN. Hatchability, weight, and selected bone and serum parameters were measured in embryos. Part of hatchlings were reared under standard conditions until 42 days, and production results, meat quality, and bone quality of broilers were determined. CCN did not show cytotoxicity to cells and chicken embryo and positively influenced bone parameters of the embryos and of broilers later (calcification) without negatively affecting the production results. Thus, the IOI of CCN could modify the molecular responses at the stage of embryogenesis, resulting in better mineralization, and could provide a sustained effect, thereby improving bone quality in adult birds.

scholarly journals The Effect of Administration of a Phytobiotic Containing Cinnamon Oil and Citric Acid on the Metabolism, Immunity, and Growth Performance of Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 399
Author(s):  
Magdalena Krauze ◽  
Monika Cendrowska-Pinkosz ◽  
Paulius Matuseviĉius ◽  
Anna Stępniowska ◽  
Paweł Jurczak ◽  
...  
Keyword(s):  
Drinking Water ◽  
Small Intestine ◽  
Citric Acid ◽  
Beneficial Effect ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Coliform Bacteria ◽  
Control Group ◽  
Cinnamon Oil ◽  
Nonesterified Fatty Acids

It was postulated that a phytobiotic preparation containing cinnamon oil and citric acid added to drinking water for chickens in a suitable amount and for a suitable time would beneficially modify the microbiota composition and morphology of the small intestine, thereby improving immunity and growth performance without inducing metabolic disorders. The aim of the study was to establish the dosage and time of administration of such a phytobiotic that would have the most beneficial effect on the intestinal histology and microbiota, production results, and immune and metabolic status of broiler chickens. The experiment was carried out on 980 one-day-old male chickens until the age of 42 days. The chickens were assigned to seven experimental groups of 140 birds each (seven replications of 20 individuals each). The control group (G-C) did not receive the phytobiotic. Groups CT-0.05, CT-0.1, and CT-0.25 received the phytobiotic in their drinking water in the amount of 0.05, 0.1, and 0.2 mL/L, respectively, at days 1–42 of life (continuous application, CT). The birds in groups PT-0.05, PT-0.5, and PT-0.25 received the phytobiotic in the same amounts, but only at days 1–7, 15–21, and 29–35 of life (periodic application, PT). Selected antioxidant and biochemical parameters were determined in the blood of the chickens, as well as parameters of immune status and redox status. The morphology of the intestinal epithelium, composition of the microbiome, and production parameters of chickens receiving the phytobiotic in their drinking water were determined as well. The addition of a phytobiotic containing cinnamon oil and citric acid to the drinking water of broiler chickens at a suitable dosage and for a suitable time can beneficially modify the microbiome composition and morphometry of the small intestine (total number of fungi p < 0.001, total number of aerobic bacteria p < 0.001; and total number of coliform bacteria p < 0.001 was decreased) improving the immunity and growth performance of the chickens (there occurred a villi lengthening p = 0.002 and crypts deepening p = 0.003). Among the three tested dosages (0.05, 0.1, and 0.25 mL/L of water) of the preparation containing cinnamon oil, the dosage of 0.25 mL/L of water administered for 42 days proved to be most beneficial. Chickens receiving the phytobiotic in the amount of 0.25 mL/L had better growth performance, which was linked to the beneficial effect of the preparation on the microbiome of the small intestine, metabolism (the HDL level p = 0.017 was increased; and a decreased level of total cholesterol (TC) p = 0.018 and nonesterified fatty acids (NEFA) p = 0.007, LDL p = 0.041, as well as triacylglycerols (TAG) p = 0.014), and immune (the level of lysozyme p = 0.041 was increased, as well as the percentage of phagocytic cells p = 0.034, phagocytosis index p = 0.038, and Ig-A level p = 0.031) and antioxidant system (the level of LOOH p < 0.001, MDA p = 0.002, and the activity of Catalase (CAT) p < 0.001 were decreased, but the level of ferric reducing ability of plasma (FRAP) p = 0.029, glutathione p = 0.045 and vitamin C p = 0.021 were increased).

scholarly journals Splenic Gene Expression Signatures in Slow-Growing Chickens Stimulated in Ovo with Galactooligosaccharides and Challenged with Heat

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 474 ◽  
Author(s):  
Elzbieta Pietrzak ◽  
Aleksandra Dunislawska ◽  
Maria Siwek ◽  
Marco Zampiga ◽  
Federico Sirri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Broiler Chickens ◽  
Physiological Saline ◽  
Negative Effects ◽  
In Ovo ◽  
Stress Related Genes ◽  
The Impact ◽  
Slow Growing ◽  
Resistance To Heat

Galactooligosaccharides (GOS) that are delivered in ovo improve intestinal microbiota composition and mitigate the negative effects of heat stress in broiler chickens. Hubbard hybrids are slow-growing chickens with a high resistance to heat. In this paper, we determined the impact of GOS delivered in ovo on slow-growing chickens that are challenged with heat. The experiment was a 2 × 2 × 2 factorial design. On day 12 of incubation, GOS (3.5 mg/egg) was delivered into the egg (n = 300). Controls (C) were mock-injected with physiological saline (n = 300). After hatching, the GOS and C groups were split into thermal groups: thermoneutral (TN) and heat stress (HS). HS (30 °C) lasted for 14 days (days 36–50 post-hatching). The spleen (n = 8) was sampled after acute (8.5 h) and chronic (14 days) HS. The gene expression of immune-related (IL-2, IL-4, IL-6, IL-10, IL-12p40, and IL-17) and stress-related genes (HSP25, HSP90AA1, BAG3, CAT, and SOD) was detected with RT-qPCR. Chronic HS up-regulated the expression of the genes: IL-10, IL-12p40, SOD (p < 0.05), and CAT (p < 0.01). GOS delivered in ovo down-regulated IL-4 (acute p < 0.001; chronic p < 0.01), IL-12p40, CAT and SOD (chronic p < 0.05). The obtained results suggest that slow-growing hybrids are resistant to acute heat and tolerant to chronic heat, which can be supported with in ovo GOS administration.

scholarly journals The use of amaranth (genus AmaranthusL.) in the diets for broiler chickens

2012 ◽  
Vol 51 (No. 7) ◽  
pp. 399-407 ◽  
Author(s):  
B. Pisarikova ◽  
Z. Zraly ◽  
S. Kracmar ◽  
M. Trckova ◽  
I. Herzig
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Control Group ◽  
Feed Conversion ◽  
Feeding Trial ◽  
Control Groups ◽  
Broiler Performance ◽  
Amaranth Grain ◽  
Percentage Yield ◽  
Experimental Group

The objective of our study was to test amaranth grain or dried biomass in the diet for broiler chickens as a resource of protein replacing animal protein. Further, the effect of amaranth on broiler performance, carcass characteristics and meat quality were assessed in a feeding trial. The experimental groups of broilers were fed feed mixtures with crude amaranth grain (AC), heat processed amaranth grain (AP) or dried above-ground biomass (AB). Control groups (C) received the diet containing animal proteins. The results obtained in the experimental groups of broiler chickens can be compared to the control group in all the production indicators under investigation. On day 42 of the experiment, live weight (g) of female broilers ranged from 2 205.1 &plusmn; 152.5 (AP) to 2 254.0 &plusmn; 136.5 (C), and that of male broilers from 2 375.1 &plusmn; 233.0 (AC) to 2 506.0 &plusmn; 286.0 (C). Feed conversion ranged from 1.80 kg in control group of male broiler chickens to 1.91 kg in experimental group of male broilers (AB). Percentage yield in female broiler chickens ranged from 70.8 &plusmn; 1.74 (AB) to 72.4 &plusmn;3.34(AC) or 72.4 &plusmn; 1.98 (C), and in male broilers from 72.8 &plusmn;3.55(AP) to 75.1 &plusmn;2.44(AB). Our results showed that amaranth can fully replace meat-and-bone meals in the diets for broiler chickens.

scholarly journals Impact of Prebiotics and Synbiotics Administered in ovo on the Immune Response against Experimental Antigens in Chicken Broilers

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 643
Author(s):  
Tadeusz Stefaniak ◽  
Jan P. Madej ◽  
Stanisław Graczyk ◽  
Maria Siwek ◽  
Ewa Łukaszewicz ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Physiological Saline ◽  
Treated Group ◽  
Delayed Type Hypersensitivity ◽  
Saline Control ◽  
Control Group ◽  
In Ovo ◽  
Humoral Immune ◽  
Air Chamber

The effect of the in ovo application of selected prebiotics and synbiotics on the humoral immune response against T-dependent (SRBC) and T-independent (dextran) antigens and delayed-type hypersensitivity (DTH) to phytohemagglutinin was studied. On the 12th day of incubation, 800 eggs (Ross 308) were divided into five groups and injected into the egg air chamber with prebiotic inulin (Pre1), Bi2tos (Pre2), a synbiotic composed of inulin and Lactococcus lactis subsp. lactis IBB SL1 (Syn1), a synbiotic composed of Bi2tos and L. lactis subsp. cremoris IBB SC1 (Syn2), and physiological saline (control group; C). The chickens were immunized twice at the 7th and 21st day of life with SRBC and dextran. A DTH test was performed on the 7th, 21st, and 35th day. The application of prebiotics and synbiotics had no significant effect on the humoral immune response. SRBC-immunized in ovo Pre1- and Pre2-treated chickens showed significantly higher serum IgG levels than the control. A significant effect on the DTH reaction was detected on the 7th (Pre1 < C) and 21st (Pre2 > Syn2) day. However; Bi2tos may transiently stimulate the cellular immune response on the 21st day. It may be concluded that the application of inulin in an egg air chamber on the 12th day of incubation may stimulate the secondary immune response. The inulin-treated group exhibited a lower mortality rate than the control group.

scholarly journals The effects of in ovo feeding of glutamine in broiler breeder eggs on hatchability, development of the gastrointestinal tract, growth performance and carcass characteristics of broiler chickens

2016 ◽  
Vol 59 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Mehdi Salmanzadeh ◽  
Yahya Ebrahimnezhad ◽  
Habib Aghdam Shahryar ◽  
Jamshid Ghiasi Ghaleh-Kandi
Keyword(s):  
Gastrointestinal Tract ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Characteristics ◽  
Abdominal Fat ◽  
Control Group ◽  
Villus Height ◽  
In Ovo ◽  
Crypt Depth ◽  
In Ovo Feeding

Abstract. The aim of the present study was to investigate the effect of in ovo feeding (IOF) of glutamine on hatchability, development of the gastrointestinal tract, growth performance and carcass characteristics of broiler chickens. Fertilized eggs were subjected to injections with glutamine (Gln) (10, 20, 30, 40 or 50 mg dissolved in 0.5 mL of dionized water) on day 7 of incubation. Hatchability, growth performance, carcass characteristics (carcass weight and relative weights of breast, thigh, heart, liver, gizzard, abdominal fat, intestine, pancreas and spleen) and jejunal morphometry (measurement of villus height and width and crypt depth) were determined during the experiment. The weight of newly hatched chickens was significantly greater in groups with Gln injection than in control and sham groups. But IOF caused lower hatchability than in the control group (non-injected eggs) (p < 0.05). Chickens from IOF of Gln showed better weight gain and feed conversion ratio (0–42 days of age), when compared to chickens hatched from control and sham groups. The IOF of Gln significantly increased villus height, villus width and crypt depth at hatch period and villus height at 42 days of age. In addition, carcass weights and relative weights of breast, thigh and gizzard were also markedly increased in chickens treated in ovo with Gln; whereas heart, liver, abdominal fat, intestine, pancreas and spleen were not significantly altered at the end of the experimental period. These data suggest that the IOF of Gln may improve jejunum development, leading to an increased nutrient assimilation and consequently to greater performance in broiler chickens.

Influence of whole wheat feeding on the development of gastrointestinal tract and performance of broiler chickens

2006 ◽  
Vol 57 (1) ◽  
pp. 21 ◽  
Author(s):  
V. Ravindran ◽  
Y. B. Wu ◽  
D. G. Thomas ◽  
P. C. H. Morel
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Broiler Chickens ◽  
Relative Size ◽  
Relative Weight ◽  
Broiler Performance ◽  
Gain Ratio ◽  
Whole Wheat ◽  
And Performance ◽  
Ground Wheat

The main aim of the present study was to investigate the changes in the relative growth of digestive organs of broilers as influenced by whole wheat feeding and age of birds. The secondary aim was to examine the effects of whole wheat feeding on broiler performance over a 5-week feeding period. Diets containing ground wheat and whole wheat (100 and 200 g/kg whole wheat replacing ground wheat during 1–21 days and 22–35 days, respectively) were placed in 4 replicate pens (46 birds/pen) each. Throughout the 5-week trial, the birds offered feed containing wheat as whole grain grew slower (P < 0.05) and consumed less (P < 0.05) feed than those offered feed containing ground wheat. Feed-to-gain ratio was not influenced (P > 0.05) by the form of wheat during the first 2 weeks, but was significantly lowered (P < 0.05) by whole wheat feeding from 3 weeks onwards. Over the 5-week period, the feed-to-gain ratio of birds fed the whole-wheat diet was 7 points better (P < 0.05) than of those fed the ground wheat diet. Improved feed efficiency from Week 3 onwards coincided with significant increases (P < 0.05) in the relative weights of the gizzard in birds fed the whole-wheat diet. Whole-wheat inclusion had no effect (P > 0.05) on the relative weights of crop, proventriculus, and pancreas or on the relative weight and length of the small intestine. The relative weights of storage sections of the gastrointestinal tract (crop, proventriculus, and gizzard) steadily declined during the 5-week period. The relative weights of pancreas and liver increased to Day 14 and decreased thereafter. The relative weights of intestinal segments were maximal during Weeks 1 and 2 of life, and declined rapidly with age. In general, the growth of different segments of the small intestine (duodenum, jejunum, and ileum) followed a similar pattern. The intestinal mass, measured as g tissue/cm tissue, steadily increased over the trial period, indicating that, although the relative size of the intestine declines with age, this decline is compensated by increased intestinal mass to support the nutrient supply function to the demand tissues.

Performance, intestinal morphometry, and incubation parameters of broiler chickens submitted to in ovo feeding with different techniques and amino acids

2019 ◽  
Vol 99 (4) ◽  
pp. 732-740
Author(s):  
Priscila Groff-Urayama ◽  
Joselaine Padilha ◽  
Suelen Einsfeld ◽  
Simone Pertile ◽  
Mateus Gorges ◽  
...  
Keyword(s):  
Amino Acids ◽  
Broiler Chickens ◽  
Relative Weight ◽  
Control Group ◽  
In Ovo ◽  
Air Chamber ◽  
Significant Difference ◽  
Hatching Eggs ◽  
Intestinal Morphometry ◽  
In Ovo Feeding

The objective of this research was to evaluate techniques, incubation period, and effects of amino acids in ovo feeding. First, 240 hatching eggs were selected and distributed in a 2 × 3 factorial arrangement (two techniques and 3 d). The parameters of incubation, relative weight of gastrointestinal organs, and classification of embryonic mortality were evaluated. In the second stage, 720 hatching eggs were incubated with five treatments: control, methionine (20 and 30 mg), and lysine (20 and 30 mg). These animals were housed during 14 d. The animals were divided with five treatments, nine replicates, and groupings based on sex. Incubation parameters, performance, intestinal morphometry, and relative weight of digestive tract organs were evaluated. The results indicate that the technique using a 45° needle axis passing through the air chamber hinders hatchability. In the organ weighing, there was no difference between the evaluated factors. For intestinal morphometry, there was only a significant difference between the dose factor for the crypt villi and crypt diameter. To conclude, the technique using a 90° angle without passing through the air chamber is safer for the embryos. The inoculation of methionine (20 and 30 mg) obtained data similar to the control group.

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