scholarly journals Aktivitas Antioksidan dan Kandungan Komponen Bioaktif Sari Buah Namnam

2015 ◽  
pp. 130-136 ◽  
Author(s):  
Adawiah Adawiah ◽  
Dede Sukandar ◽  
Anna Muawanah
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Oxidative Reaction ◽  
High Antioxidant Activity

Antioxidant is a compoud  inhibit that have an important role in protecting health due to it can absorb free radical molecules and inhibit oxidative reaction which cause anykinds of diseases. This study aims to determine the chemical compositition and antioxidant  activity of namnam juice, characteristic of namnam. In this experimental, proximat analysis, TPC, TFC, Vitamin C, antioxidant activity were evaluated. Namnam juice contains 996.03 mg/L of phenolics, 421.09 mg/Lof flavonoids, 121.44 mg/100mL of vitamin C and high antioxidant activity with IC50 5µL/mL.DOI :http://dx.doi.org/10.15408/jkv.v0i0.3155.

scholarly journals Synthesis and Antioxidant of Some New Pyrazolo[1,5-a]pyrimidine, Pyrazolo[5,1-b]quinazoline and Imidazo[1,2-b]pyrazole Derivatives Incorporating Phenylsulfonyl Moiety

2021 ◽  
Vol 10 (3) ◽  
pp. 2414-2428
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Protective Effects ◽  
Oxidative Hemolysis ◽  
Elemental Analyses ◽  
Phenolic Group ◽  
High Antioxidant Activity ◽  
New Compounds

The synthesis and antioxidant of some new pyrazole analogs were described. It is achieved by the reaction of phenyl-4-(phenylsulfonyl)-1H-pyrazole-3,5-diamine (3) with different bifunctional reagents. The free radical-induced damage and the protective effects of the newly synthesized pyrazoles were studied. These new compounds inhibit the free radical-induced oxidative hemolysis of red blood cells effectively. It was found that these compounds effectively inhibit the free radical-induced oxidative hemolysis of red blood cells. Compound 5, which contain phenolic group, and 17, which bear sulfur, nitrogen atoms, and benzothiazole ring, respectively displayed high antioxidant activity. Analogs 15, 11, 10, and 9 were proved to exhibit antioxidative activity. Structures of new pyrazoles were confirmed by spectroscopic and elemental analyses and have been screened for their antioxidant activity.

PERBANDINGAN METODE EKSTRAKSI MASERASI DAN SOKLETASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN JAMBU BOL (Syzygium malaccense L.)

2017 ◽  
Vol 3 (1) ◽  
pp. 91
Author(s):  
Henny Nurhasnawati ◽  
Sukarmi Sukarmi ◽  
Fitri Handayani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Extraction Methods ◽  
Natural Antioxidant ◽  
Comparison Of Methods ◽  
Activity Test ◽  
Ic50 Value ◽  
The Difference ◽  
Free Radical Activity

Malay apple (Syzygium malaccense L.) is a plant that can be used for treatment. Antioxidants have the activity to reduce free radical compounds which is one of the causes of the emergence of various diseases in humans. The purpose of this study to determine the comparison of methods of maceration and socletation extraction of antioxidant activity of malay apple leaf. Research stages include sampling, plant determination, making of simplisia, maceration extraction and socletation with 70% ethanol solvent and determine the antioxidant activity by UV-Vis spectrophotometry with DPPH (1,1-diphenyl-2-picrylhydrazyl) as free radical. The parameter is IC50 value that is the concentration of antioxidant compound which can cause 50% loss of DPPH free radical activity. Although both methods show very strong antioxidant activity, it can be concluded that the difference in extraction methods has an effect on the antioxidant activity produced. The results of antioxidant activity test showed that socletation methods gave an average IC50 value of 37.67 ppm, this value is higher than maceration methods with an average IC50 value of 47.80 ppm. Malay apple leaf has the potential as a natural antioxidant, although it has a lower IC50 value than vitamin C which is 9.72 ppm.

scholarly journals Partition Behavior in Aqueous Two-Phase System and Antioxidant Activity of Flavonoids from Ginkgo biloba

2018 ◽  
Vol 8 (11) ◽  
pp. 2058 ◽  
Author(s):  
Can Liu ◽  
Shuochen Liu ◽  
Lingli Zhang ◽  
Xiaoxue Wang ◽  
Lanqing Ma
Keyword(s):  
Antioxidant Activity ◽  
Polyethylene Glycol ◽  
Free Radical ◽  
Vitamin C ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phase System ◽  
Two Phase ◽  
Extraction Parameters ◽  
Partition Behavior

In this work, the aqueous two-phase extraction parameters and in vitro antioxidant activity of total flavonoids from Ginkgo biloba were investigated. The partition behavior of the flavonoids in an aqueous two-phase system (ATPS) was analyzed, the extraction parameters were optimized using response surface methodology, and the antioxidant activity of the flavonoids was determined by free-radical scavenging tests (1,1-diphenyl-2-picrylhydrazyl (DPPH) assay) and the ferric reducing/antioxidant power (FRAP) assay. The results showed that the concentration of ammonium sulfate was negatively correlated with the phase-volume ratio, whereas the concentration of polyethylene glycol was positively correlated. The maximum yield of flavonoids (4.11 mg g−1) was obtained under the following optimal extraction conditions: Concentration of polyethylene glycol (PEG)1500 30% and (NH4)2SO4 22% (mass fraction), and liquid/solid ratio 40:1 (mL g−1). The antioxidant activity tests showed that the flavonoids from the G. biloba leaf exhibited free-radical scavenging activity, with an IC50 of 2.66 mg L−1, which was superior to that of vitamin C. The free-radical scavenging ability of the flavonoids was proportional to the flavonoid concentration. The total reducing power of the Ginkgo flavonoids was slightly lower than that of vitamin C. In this study, the distribution of flavonoids in an ATPS was analyzed and a mathematical model for the ATPS extraction of Ginkgo flavonoids was established, which provides a reference for further development and utilization of G. biloba.

scholarly journals Antioxidant Activity of Indian Propolis - An In Vitro Evaluation

2016 ◽  
Vol 5 ◽  
pp. 79-85 ◽  
Author(s):  
Shubharani Ramnath ◽  
Sivaram Venkataramegowda
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Tamil Nadu ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Activity Level ◽  
In Vitro Assays ◽  
Antioxidant Compounds

Antioxidants from the natural products are essential to prevent the progression of free radical mediated diseases. In the present study, ethanol extract of propolis collected from different geographical origin were evaluated for their free radical scavenging potential by employing different in-vitro assays such as DPPH, ABTS, Nitric oxide and Hydrogen peroxide. All the tested samples contained considerable amount of total phenols and vitamin C content. In the entire assay, the percentage of inhibition increased with the increase in concentration. Among the propolis samples collected, the highest activity was found in Tamil Nadu, Kerala, Karnataka and Haryana. The difference in the antioxidant activity level was obtained from the assay may reflect a relative difference in the ability of antioxidant compounds to scavenge different free radicals in the extract. Phenols and vitamin C are the major contributors to antioxidant activity in propolis. The propolis from these locations may be of considerable interest in preventing the ill effects of excessive free radical generation in the human body.

scholarly journals ANTIOXIDANT ACTIVITY TEST of SIJUKKOT (Lactuca indica L.) LEAVES EXTRACT LEAF

2020 ◽  
Vol 3 (2) ◽  
pp. 49
Author(s):  
Sumiati Nainggolan ◽  
Ida Duma Riris
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
A Value ◽  
Activity Test ◽  
Radical Method ◽  
Standard Of Comparison ◽  
Lactuca Indica

Has been investigated the antioxidant activity of  sijukkot ( Lactuca indica L.) The antioxidant activity was tested by capturing 2,2’-diphenyl-picrylhydrazyl (DPPH) free radical method and Vitamin C as a standard of comparison, to absorption using UV-Vis  spectrophotometry at a wavelength of 517nm. The data obtained were analyzed using the SPSS for windows, resulting in a value of y = 0.2036x + 30.35 for sijukkot extract and y = 29.625x - 73.664 for vitamin C. It was found the leaves have an antioxidant site with 96,51 ppm through IC50 test 4,17 ppm which used Vitamin C as a control. 

scholarly journals Design of Healthy Snack Based on Kiwifruit

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3309 ◽  
Author(s):  
Urszula Tylewicz ◽  
Malgorzata Nowacka ◽  
Katarzyna Rybak ◽  
Kinga Drozdzal ◽  
Marco Dalla Rosa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Freeze Drying ◽  
Bioactive Compound ◽  
Convective Drying ◽  
Drying Methods ◽  
Hot Air ◽  
Sugar Addition ◽  
High Antioxidant Activity

Kiwifruit is an excellent source of vitamin C and other bioactive compounds, which contribute to its high antioxidant activity. However, the fruits with small size and low weight are considered waste and are unprofitable; therefore, the production of healthy kiwifruit-based dried snacks, which contain a lot of health-beneficial ingredients, could be a viable alternative for their use. The aim of this study was to develop formulations and methods to produce attractive and nutritionally valuable dried snacks based on yellow kiwifruit. Three different puree formulations (kiwifruit; fennel; and strawberry, lemon, or spinach) with or without addition of sugar were subjected to two drying methods: freeze-drying (fruit bars) and conventional hot air drying (fruit leathers). The obtained products were analysed for their content of total polyphenols (TPs), flavonoids, and vitamin C, as well as their antioxidant activity. The results showed that snacks prepared by freeze-drying (fruit bars) presented higher TP, vitamin C, and flavonoids content than those prepared by convective drying; however, the antioxidant activity did not always follow this trend. The amount of bioactive compounds depended on the formulation used for the preparation of snacks. The effect of the sugar addition seems to be strictly related to the mix used and specific bioactive compound investigated.

scholarly journals Pengaruh Metode Pengeringan Terhadap Aktivitas Antioksidan Ekstrak Etanol

2019 ◽  
Vol 3 (1) ◽  
pp. 44-52
Author(s):  
Endra Pujiastuti ◽  
Rahma Sani Saputri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethanol Extract ◽  
Drying Methods ◽  
Drying Method ◽  
Oven Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Qualitative Test ◽  
High Antioxidant Activity

Drying is the most important step to keep the compound stability in simplicia and is very influential to produce good quality igredients in the antioxidant activity contained in the simplicia. Antioxidants are oxidation-free inhibitors of free radical reactions. Parijoto is one of the plants that have antioxidant compounds.This study aims to determine the effect of drying, concentration of antioxidant activity of parijoto (Medinilla speciosa Blume) fruit. The drying method used is direct sunlight drying, indirect sunlight and oven. The antioxidant activity of ethanol extract on drying method was determined by 2,2 diphenyl-1-picrylhidrazyl (DPPH) method which is free radical. The qualitative test DPPH showed that the higher levels of ethanol extract from the three drying methods further diminished the color of DPPH solution in ethanol.The result showed thet the drying method gave different effect to the antioxidant activity of parijoto fruit which is significant(p0,05). Drying methods wich have high antioxidant activity are given by drying using oven method with an IC50 value 33,75µg/ml.

scholarly journals Pengaruh Perendaman Asam Askorbat dan Natrium Bisulfit pada Dua Varietas Bawang Merah (Allium ascalonicum L.) terhadap Sifat Fisikokimia dan Organoleptik Tepungnya (Effect of Ascorbic Acid Soaking and Sodium Bisulphite on Two Varieties of Shallot (Allium ascalonicum L.) on Physical and Organoleptical Character of Shallot Powder)

2016 ◽  
Vol 36 (03) ◽  
pp. 270 ◽  
Author(s):  
Ermi Sukasih ◽  
Setyadjit Setyadjit
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Vitamin C ◽  
Moisture Content ◽  
Acid Treatment ◽  
Total Phenolics ◽  
Ash Content ◽  
Sodium Bisulphite ◽  
Significant Data

Recently, there is an increase of shallot production in Indonesia. One alternative of flesh shallot processing is shallot powder making which can be used during the off season. The aim of the study was to observe the effect of immersion in ascorbic acid and sodium bisulphite on two varieties of shallot on physical and organoleptic characters of shallot powder. Drying was done using tray dryer. Experimental design used was factorial completely randomised design. The first factor was the type of immersion agent and duration time and the second factor was shallot varieties (Bima and Sembrani). Statistical mean test was using Tukey Honestly Significant (HSD). The result showed that the treatments significantly affected parameters such as moisture content, ascorbic acid, total phenol, anthocyanin, and antioxidant activity, free radical inhibition and color (chroma). From ranking test the best treatment was Bima variety with 0.2% ascorbic acid treatment for 30 min. Characteristic of the best treatment was with moisture content 4.03±0.79 % (w/w), ash content 4.45±0.13 % (w/w), fat content 1.24±0.64 % (w/w), protein content 15.56±0.28 % (w/w), 61.94±4.11 mg/100g, total phenolics 256.39±16.26 ppm, anthocyanins 50.70±2.31 ppm, quercetin 2612.40±408.94 ppm, antioxidant activity ekuivalent, 225.72±4.0 µg/mL, inhibition free radical 83.05±1.69 %, 85.02 hue value (yellowish red) and aroma score 3.2 (strong), brightness score 4.2 (bright) and color 1.25 (pale red). ABSTRAKKenaikan cukup signifikan pada produksi bawang merah terjadi di Indonesia. Salah satu alternatif pengolahan bawang merah segar adalah melalui pengolahan menjadi tepung sehingga tetap tersedia saat langka dan harganya tinggi. Penelitian ini bertujuan untuk melihat pengaruh perendaman asam askorbat dan natrium bisulfit pada dua varietas bawang merah terhadap sifat fisikokimia dan organoleptik tepung yang dihasilkan. Pengeringan dilakukan dengan tray dryer. Percobaan menggunakan rancangan acak lengkap faktorial, faktor pertama jenis bahan  dan lama perendaman dan faktor kedua adalah varietas bawang merah (Bima dan Sembrani). Analisis statistik menggunakan metode Tukey Honestly Significant Data (HSD). Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata terhadap parameter kadar air, vitamin C, total fenolik, antosianin, aktivitas antioksidan, inhibisi radikal bebas dan warna (chroma). Berdasarkan uji peringkat diperoleh perlakuan terbaik adalah tepung bawang merah dari varietas Bima dengan perlakuan perendaman asam askorbat 0,2 % selama 30 menit. Karakteristik dari tepung bawang ini memiliki  kadar air 4,03 ± 0,79 % (bb), kadar abu 4,45 ± 0,13 % (bb), lemak 1,24 ± 0,64 % (bb) protein 15,56 ± 0,28 % (bb), vitamin C 61,94 ± 4,11 mg/100 g, total fenol 256,39 ± 16,26 ppm, antosianin 50,70 ± 2,31 ppm, kuersetin 2612,40 ± 408,94 ppm, aktivitas antioksidan ekuivalen 225,72 ± 4,07 µg/mL, inhibisi radikal bebas 83,05 ± 1,69 %, nilai oHue 85,02 (kuning kemerahan) dan skor aroma 3,2 (kuat), skor kecerahan 4,2 (terang) dan skor warna 1,25 (merah pucat).   Kata kunci: Asam askorbat; tepung bawang merah; perendaman; natrium bisulfit

scholarly journals Identifikasi Senyawa Kimia Aktif Antioksidan Dari Ekstrak Etil Asetat Buah Andaliman (Zanthoxylum acanthopodium DC)

2018 ◽  
Vol 1 (3) ◽  
pp. 162-166
Author(s):  
Wiwi Winarti ◽  
Partomuan Simanjuntak ◽  
Muhammad Fahmi Syahidin
Keyword(s):  
Antioxidant Activity ◽  
Fourier Transform ◽  
Free Radical ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Positive Control ◽  
Fruit Extract ◽  
Stomach Pain ◽  
Infra Red ◽  
Ft Ir

Buah andaliman, (Zanthoxylum acanthopodium DC),banyak dikonsumsi oleh masyarakat sebagai bumbu makanan dan obat tradisional seperti obat sakit perut, tonikum, dan anti mikroba. Penelitian ini bertujuan untuk mengidentifikasi senyawa yang berkhasiat sebagai antioksidan dari ekstrak buah andaliman. Ekstraksi dilakukan secara maserasi kinetik menggunakan pelarut n-heksan, dan etil asetat. Ekstrak kental yang diperoleh kemudian diuji aktivitas antioksidan dengan metode peredaman radikal bebas menggunakan DPPH. Sebagai kontrol positif digunakan vitamin C. Selanjutnya dilakukan pemurnian dengan kromatografi kolom sampai diperoleh isolat EA.X.6.1 yang mempunyai aktivitas antioksidan tertinggi. Hasil uji aktivitas antioksidan dengan metode peredaman radikal bebas DPPH (1,1-difenil-2-pikrilhidrazil) menunjukkan bahwa ekstrak etil asetat buah andaliman mempunyai aktivitas antioksidan dengan nilai IC50 sebesar 66,91 bpj, dan isolat EA.X.6.1 yang memiliki aktivitas antioksidan tertinggi dengan nilai IC50 sebesar 135,58 bpj. Berdasarkan hasil analisis dengan spektrofotometri UV-Vis, spektrofotometri Fourier Transform Infra Red (FT- IR) dan Kromatografi Gas-Spektrometri Massa (KG-SM) diduga senyawa aktif antioksidan yang terdapat dalam ekstrak etil asetat buah andaliman adalah 2-metoksi-4-vinilfenol.   Andaliman fruit, (Zanthoxylum acanthopodium DC), is widely consumed by people as a food spice and traditional medicine to treat stomach pain, tonic, and as anti-microbial drugs. This study aimed to identify compounds that wereefficacious as antioxidants of andaliman fruit extract. The extraction was performed by kinetic maceration method using n-hexane and ethyl acetate. Then, the crude was tested for its antioxidant activity by free radical DPPH scavenging method. Vitamin C was used as  a positive control. Furthermore, the extract was purified by column chromatography to obtain isolate  EA.X.6.1 which has the highest antioxidant activity. The results of evaluation on antioxidant activity by free radical DPPH (1,1-difenil-2-pikrilhidrazil)scavenging method showed that ethyl acetate andaliman fruit extract revelaed antioxidant activity with IC50value of 66,91 bpj and isolate EA.X.6.1 which showed the highest antioxidant activity with IC50value of 135,58 bpj. Based on analysis using UV-Vis spectrophotometer, spectrophotometer Fourier Transform Infra Red (FT- IR) and Gas Chromatography- Mass Spectrophotometry (GC-MS), it was considered that antioxidant chemical active compound in ethyl acetate andaliman fruit extract was 2-methoxy-4-vinylphenol

Optimization of preparation of rosehip tea from the area of Stara Planina Mt

Etnobotanika ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 57-69
Author(s):  
Danijela Kostić ◽  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Therapeutic Effects ◽  
Bioactive Substances ◽  
Rosa Canina ◽  
Maximum Extraction ◽  
High Antioxidant Activity ◽  
Medicinal Properties

Stara Planina Mt is rich in medicinal plants, that are natural sources of various bioactive substances and have many useful medicinal properties. Rosehip, the fruit of wild rose (Rosa canina L.) is very popular and widely used. There is extensive empirical evidence of its therapeutic effects. The high content of vitamins, organic acids, phenolic compounds, carotenoids and pleasant taste define it as a valuable dietary supplement. There are different procedures for making rosehip teas. In this paper, we have optimized the process of preparation of rosehip tea from the aspect of maximum extraction and preservation of vitamin C content in tea. The antioxidant activity of aqueous extracts of rosehips, depending on the extraction time and the temperature at which the extraction is performed, was also examined..The free radical scavenging activity of the plant extracts was analyzed by using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay. Optimal conditions are 30 minutes and a temperature of 40 °С. In this way, vitamin C is best extracted and its stability is preserved, which results in the high antioxidant activity of the tested teas.

Sign in / Sign up

Export Citation Format

Share Document