Regenerative endodontics model in immature infected rat molars using two step protocol

Aim: to develop a model for regenerative endodontics using newly-weaned Wistar rats immature molars with pulp necrosis to histologically describe the evolution of apical tissues following treatment with a bi-antibiotic paste, induced bloodclot formation and MTA. Methods: Ten 25-day-old female Wistar rats were divided into an initial control group (Ci) and two experimental groups in which pulp necrosis was experimentally induced on the left mandibular first molar by exposing the pulp chamber and leaving it open to the oral environment. One of the experimental groups was left untreated (E1) while the other was submitted to a protocol of regenerative endodontics 10 days thereafter (E2). Fifteen days after placement of a bi-antibiotic paste, bleeding was induced into the root canal space and MTA was placed upon. Animals were euthanized 30 days later. Right mandibular first molars served as an 80-day-old final control group (Cf). Each hemimandible was histologically processed to analyse parameters associated with root development. Statistical analysis was carried by means of ANOVA; p values below 0.05 were considered statistically significant. Results: baseline (i.e. 25-days old) mean root length and apical diameter of the distal root canal were 1.84±0.25 and 0.38±0.02mm respectively. Following the regenerative endodontic protocol, cells lining the walls of the root canal and significant increase to both length (2.37±0.22mm) and diameter (0.32±0.03 mm) were observed. Conclusions: newly-weaned Wistar rats serve as a suitable model to evaluate regenerative endodontic protocols. However, further research is needed in order to disclose the nature of the cells and/or cell mediators involved.

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ANTIFUNGAL EFFECT OF NIGELLA SATIVA EXTRACT ON FEMALE WISTAR RATS VULVOVAGINAL CANDIDIASIS MODEL

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i3.38308 ◽

2020 ◽

pp. 66-68

Author(s):

MUHAMMAD RUSDA

Keyword(s):

Candida Albicans ◽

Therapeutic Effect ◽

Wistar Rats ◽

Nigella Sativa ◽

Vulvovaginal Candidiasis ◽

Analytic Study ◽

Control Group ◽

Antifungal Effect ◽

Female Wistar Rats

Objective: To assess the antifungal effect of Nigella sativa extract on female Wistar rats vulvovaginal candidiasis. Methods: This was an analytic study that assess the therapeutic effect of Nigella sativa in rats’ vulvovaginal candidiasis model. The subjects were 28 rats that had been inoculated with Candida albicans and divided into 4 groups: control group (G1), fluconazole group (G2), Nigella sativa group (G3) and combination of Nigella sativa with fluconazole group (G4). Candida albicans colony was measured to assess the therapeutic effect of the treatment. Results: There were no difference number of Candida albicans colony between all group before inoculation (p = 0,274) and after inoculation (p = 0,323). There were a significant decreased number of Candida albicans colony on the 72 h after the treatment between the three types of treatment with the control group (Nigella sativa group p = 0,002; Fluconazole group p = 0,001; Nigella sativa+fluconazole p = 0,001) Conclusion: Nigella sativa has a potential antifungal effect by reducing the number of Candida albicans colonies.

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Comparison of the absorption efficiency of α- and β-cryptoxanthin in female Wistar rats

British Journal Of Nutrition ◽

10.1017/s0007114507336751 ◽

2007 ◽

Vol 97 (2) ◽

pp. 329-336 ◽

Cited By ~ 2

Author(s):

Dietmar E. Breithaupt ◽

Elhadi M. Yahia ◽

Francisco J. Valdés Velázquez

Keyword(s):

Treatment Group ◽

Wistar Rats ◽

Corn Oil ◽

Absorption Efficiency ◽

Array Detector ◽

Control Group ◽

Plasma Samples ◽

Female Wistar Rats ◽

Positive Correlations ◽

Rat Livers

Xanthophylls, such as lutein and zeaxanthin, have received increasing interest in recent years because of positive correlations between their consumption and the prevention of eye diseases. Numerous human intervention studies have been conducted with lutein to estimate the bioavailability from different formulations. The present study was designed to obtain basic data on the absorbance efficiency of the monohydroxylated counterparts of lutein and zeaxanthin: α- and β-cryptoxanthin. A corn-oil-based diet comprising ß-cryptoxanthin from papaya purée and α-cryptoxanthin from green carrot leaves was fed to five female Wistar rats for 8 consecutive days at a rate of 17·3 nmol/d and 9·2 nmol/d, respectively. The identity of the xanthophylls in the supplement was ascertained by LC-(APCI)MS analyses, and xanthophylls present in liver and plasma samples were determined by HPLC/diode array detector (DAD). The β-cryptoxanthin concentrations of rat livers in the treatment group were statistically distinguishable (P < 0·01) from those present in the livers of the control group that were fed a basic diet. α-Cryptoxanthin, the second xanthophyll present in the supplement, was not found in rat livers in the treatment group. Plasma samples were free of xanthophylls. This is the first report proving that β-cryptoxanthin has a higher absorption efficiency than α-cryptoxanthin in rats, at least from a minimally processed oil-based xanthophyll supplement.

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Fluoxetine effect on gestation and fetal development

Acta Medica Marisiensis ◽

10.2478/amma-2014-0034 ◽

2014 ◽

Vol 60 (4) ◽

pp. 157-159

Author(s):

Bianca Eugenia Ösz ◽

C. E. Vari ◽

Maria Dogaru

Keyword(s):

Body Weight ◽

Weight Gain ◽

Fetal Development ◽

Wistar Rats ◽

Selective Serotonin Reuptake Inhibitors ◽

Gravid Uterus ◽

Control Group ◽

Increased Risk ◽

Female Wistar Rats ◽

In Pregnancy

Abstract The prenatal exposure to selective serotonin reuptake inhibitors (SSRIs) is very controversial. There is no conclusive evidence for increased risk of malformations after SSRI use in pregnancy. The aim of the study was to determine how fluoxetine is affecting gestation and fetal development in rats. Twenty sexually mature female Wistar rats weighting between 250-260 g received 20 mg/kg body weight fluoxetine from the first day of gestation and during the entire gestation period.The drug was administered by oral route. Healthy, primipareus animals were selected along with 20 female Wistar rats, as control group. Mature males were caged with virgin females for an entire week. Rat’s behaviour during gestation, after birth and rats body weight was examined. The number of healthy pups was also noted. The females not giving birth after 21 days to any pup were anesthetized (halothane through gas scavenging apparatus untilled death) and the gravid uterus were dissected out and examined. Compared to the controlled group, in which weight gain was more significant, the animals from the experimental group had a slight increase in body weight. The weight gain normally induced by gestation, is less significant in fluoxetine treated rats due to the increase serotonin levels in the brain. The uteri examination of pregnant rats showed an increase in the number of dead and resorbed rat embryos. Preclinical studies suggest that the inclusion of fluoxetine in pregnancy category C is justified and the appropriateness of its administration in pregnancy is still an unresolved issue.

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Mung bean sprout extract suppresses Monosodium Glutamate (MSG) effect on the reproductive hormones (FSH and Estrogen) in female Wistar rats

Majalah Obstetri & Ginekologi ◽

10.20473/mog.v1i12019.24-27 ◽

2019 ◽

Vol 27 (1) ◽

pp. 24 ◽

Cited By ~ 1

Author(s):

Widati Fatmaningrum ◽

Woro Setia Ningtyas

Keyword(s):

Rattus Norvegicus ◽

Mung Bean ◽

Wistar Rats ◽

Monosodium Glutamate ◽

Follicle Stimulating Hormone ◽

Control Group ◽

Bean Sprout ◽

Mung Bean Sprouts ◽

Female Wistar Rats ◽

Bean Sprouts

Objectives: The purpose of this study was to analyze the effects of mung bean sprout extracts on Follicle stimulating hormone (FSH) and estrogen hormone in female Wistar rats (Rattus norvegicus) exposed to monosodium glutamate (MSG).Materials and Methods: This true experimental study was conducted by using post-test only control group design in the laboratory for animal experimentation of Faculty of Veterinary Medicine - Airlangga University in which Wistar rats (Rattus norvegicus) aged 2 months weighing 150-200 grams were used in this experiment. The samples comprised of 5 rats distributed in each group, totaling 7 groups. I Control Group (P1) was provided with Aquades for 37 days; II (P2) was provided with Aquades for 7 days + MSG 0.03 mg/g of weight on day 8-37; III (P3) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0,03mg/g of weight; IV (P4) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.03mg/g of weight; V (P5) was provided with Aquades for 7 days + MSG 0.7 mg/g of weight on day 8-37; VI (P6) was provided with extract of mung bean sprouts 72mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight; VII (P7) was provided with extract of mung bean sprouts 144mg/200g of weight on day 1-37 + MSG 0.7mg/g of weight.Serum samples were taken for Follicle Stimulating Hormone (FSH) and Estrogen with ELISA method. Data analysis to test the differences between groups was done by using one way Anova statistical test.Results: MSG dosage 0,03mg/gBB or 0,7mg/g BB was significant difference in FSH (p = 0,011) and estrogen (p = 0,008).Conclusion: obtained from this research that giving green bean sprout extract influence to the level of FSH and estrogen hormone.

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Methyl Gallate Attenuates Doxorubicin-Induced Cardiotoxicity in Rats by Suppressing Oxidative Stress

Scientifica ◽

10.1155/2021/6694340 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Akheruz Zaman Ahmed ◽

Shakta Mani Satyam ◽

Prakashchandra Shetty ◽

Melanie Rose D’Souza

Keyword(s):

Oxidative Stress ◽

Normal Control ◽

Wistar Rats ◽

Group Iv ◽

Control Group ◽

Methyl Gallate ◽

Group Iii ◽

Group I ◽

Female Wistar Rats ◽

Group Ii

Doxorubicin-induced cardiotoxicity is the leading cause of morbidity and mortality among cancer survivors. The present study was aimed to investigate the cardioprotective potential of methyl gallate; an active polyphenolic nutraceutical, against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats (150–200 g) were divided into four groups (n = 6) which consist of normal control (group I), doxorubicin control (group II), test-A (group III), and test-B (group IV). Group III and group IV animals were prophylactically treated with methyl gallate 150 mg/kg/day and 300 mg/kg/day orally, respectively, for seven days. Doxorubicin (25 mg/kg; single dose) was administered through an intraperitoneal route to group II, III, and IV animals on the seventh day to induce acute cardiotoxicity. On the 8th day, besides ECG analysis, serum CK, CK-MB, LDH, AST, MDA, and GSH were assayed. Following gross examination of isolated hearts, histopathological evaluation was performed by light microscopy. A significant ( p < 0.05) cardiac injury, as well as oxidative stress, was observed in doxorubicin control rats in comparison to normal control rats. Methyl gallate at both the doses significantly ( p < 0.05) reduced doxorubicin-induced ECG changes, dyslipidaemia, and elevation of CK, CK-MB, LDH, AST, MDA and increased GSH level. Methyl gallate reversed the doxorubicin-induced histopathological changes in the heart. The present study revealed that methyl gallate exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats by suppressing oxidative stress. Our study opens the perspective to clinical studies for consideration of methyl gallate as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

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Haematological Study on the Effects of Goko Cleanser (Herbal Mixture) on Adult Female Wistar Rats

International Blood Research & Reviews ◽

10.9734/ibrr/2021/v12i130141 ◽

2021 ◽

pp. 8-19

Author(s):

Darlington Nnamdi Onyejike ◽

McWilliams, Winifred Chidera ◽

Mmaju, Chidinma Ifeyinwa ◽

Okeke, Somadina Nnamdi ◽

Obiesie, Ifechukwu Justicia ◽

...

Keyword(s):

Adult Female ◽

Wistar Rats ◽

The Body ◽

Control Group ◽

Herbal Mixture ◽

Female Wistar Rats ◽

Group 3 ◽

Haematological Study ◽

The Brain ◽

Herbal Mixtures

Introduction: Goko cleanser is one of the popular herbal mixtures used by the Nigerian populace. This study was carried out to evaluate the effects of this herbal mixture on some haematological parameters of adult female Wistar rats. Methodology: A total of twenty five Wistar rats weighing about 160 – 280g were divided into five groups according to their corresponding weights. The groups were designated as groups 1 – 5. Group 1 served as the control group, while groups 2, 3, 4, and 5 received 1000mg/Kg, 1500mg/Kg, 2000mg/Kg and 4000mg/Kg respectively. Results: The TWBC, PCV, PLATELET count, and RBC and Hb showed a statistically significant (p<0.05) increase for the test groups 2 – 5 when compared to the control group. The body weight had a statistically significant (p<0.05) decrease in groups 2, 4, and 5; while group 3 had a statistically insignificant (p>0.05) decrease. Conclusion and Recommendation: This study revealed that Goko Cleanser contains some haemopoietic benefits, but inhibit appetite centres. Hence, this study recommends that further study be carried out on the effects of this herbal mixture on the brain so as to reveal the extent of its effect on the appetite centres.

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Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome

International Journal of Infertility & Fetal Medicine ◽

10.5005/jp-journals-10016-1104 ◽

2015 ◽

Vol 6 (2) ◽

pp. 67-72

Author(s):

Manizheh Karami ◽

Fatemeh Lakzaei ◽

MohammadReza Jalali Nadoushan

Keyword(s):

Nitric Oxide ◽

Polycystic Ovary Syndrome ◽

Wistar Rats ◽

Polycystic Ovary ◽

Treated Group ◽

Female Rats ◽

Control Group ◽

Ovary Syndrome ◽

Crown Rump Length ◽

Female Wistar Rats

ABSTRACT Background and objective Polycystic ovary syndrome (PCOS) can be induced in Wistar rats by over production of nitric oxide (NO). This study evaluated the efficacy of naloxone on the breeding characteristics of rats suffering from nitric oxide induced PCOS. Materials and methods Twenty-four female Wistar rats(200–250 gm) were kept as virgin under standard conditions. They were divided into four groups (n = 6). One group of the animals received L-arginine (50 mg/kg) intraperitoneally (i.p.) for 9 days/once a day. Another group was administered naloxone hydrochloride (0.4 mg/kg, i.p.) prior to injection of L-arginine. The third group was injected solely naloxone. Control group received saline solution (1 ml/kg, i.p.). After the treatments, all female rats were coupled with the intact males. They were then separated by observation of vaginal plaques; it was considered as day 0 of pregnancy. Eventually, they were operated on days 18 to 19 of the gestation to collect the animals’ ovaries. The samples were studied for pathological evidence. The fetal number and weight along with the fetal crown-rump length (CRL) were measured. Results The ovaries obtained from the L-arginine treated group had large cysts with thickened granulosa cell layer in contrast to those of the control or naloxone treated rats (p < 0.0001). The number of fetus though showed a decrease in the L-arginine treated rats (3 ± 1), but the fetal weight or fetal CRL did not change (p > 0.05). Conclusion This study may clearly illustrate the polycystic characteristics in the L-arginine treated group. It may particularly display the breeding efficacy of naloxone in rats with PCOS. How to cite this article Karami M, Lakzaei F, Nadoushan MRJ. Naloxone Breeding Effectiveness in Rat Suffering from Nitric Oxide-induced Polycystic Ovary Syndrome. Int J Infertil Fetal Med 2015;6(2):67-72.

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Evaluation of the toxic influence of vitamin E (dl-alpha-tocopheryl acetate) and treatment with aqueous extracts of cinnamon or anise on lipid profile and liver functions of female wistar rats

International Journal of Medicine ◽

10.14419/ijm.v5i2.8315 ◽

2017 ◽

Vol 5 (2) ◽

pp. 248

Author(s):

Sabah Ibrahim ◽

Murwan Sabahelkhier

Keyword(s):

Vitamin E ◽

Wistar Rats ◽

Low Dose ◽

Density Lipoprotein ◽

Control Group ◽

High Dose ◽

Toxic Dose ◽

Liver Functions ◽

Female Wistar Rats ◽

Better Than

The toxic effects of vitamin E and its treatment with aqueous extracts of Cinnamon or anise on lipid profile and liver functions of female wistar rats were examined for six weeks during September 2016 at labs in al-Neelain University. 18 rats were divided into six groups: 1. negative control group (sunflower oil), 2. Positive control group and rest groups given (1500 mg/Kg/BW/day of Vitamin E). After two hours, the four treated groups received a low dose (2.13g/Kg) and a high dose (3.20g/Kg) 20g/Kg from Cinnamon aqueous extract (CAE) and Anise aqueous extract (AAE). At the end rats were sacrificed then serum and liver tissues were analyzed. Vitamin E toxic dose had caused a significant increase in serum Triglycerides (TG), Alanine amino transferase (ALT) levels, while it decreased the levels of High-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC) and Aspartate amino transferase (AST). All treatments decreased TG and ALT levels. CAE low dose significantly increased TC, LDL and HDL levels. CAE high dose caused a significant decrease in AST, TC, and LDL. Both doses of AAE, caused significant increases on AST levels, and only anise low dose caused a significant decrease on TC and LDL levels. Vitamin E toxic dose caused severe fatty change in liver histology, which was near normal in both doses of CAE with a small necrosis in a low dose. Only AAE low dose normalized the liver. To conclude Vitamin E oral administration with a dose of (1500 mg/Kg) induced liver injury with an elevation in ALT and TG levels, which was significantly ameliorated by both treatments. Cinnamon was better than anise in ameliorating the toxicity. Cinnamon high dose was better than Cinnamon low dose; in contrast anise low dose was better than Anise high dose.

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Gossypol Promotes Degeneration of Ovarian Follicles in Rats

The Scientific World JOURNAL ◽

10.1155/2014/986184 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Ivana Cristina Nunes Gadelha ◽

Michelly Fernandes de Macedo ◽

Sílvia Catarina Salgado Oloris ◽

Marilia Martins Melo ◽

Benito Soto-Blanco

Keyword(s):

Estrous Cycle ◽

Wistar Rats ◽

Serum Levels ◽

Ovarian Follicles ◽

The Other ◽

Control Group ◽

Stages Of Development ◽

Hormone Levels ◽

Stage Of Development ◽

Female Wistar Rats

The present study aimed to determine if gossypol interferes with ovarian follicles in rats. Twenty-four female Wistar rats were assigned to two equal groups: one control group and the other dosed with gossypol (25 mg/kg/day, subcutaneously) for 15 days. Ovarian follicles were histologically classified according to the stage of development and as normal or atretic. Gossypol treatment reduced the length of estrous with an increase in the duration of the diestrus phase. This compound was responsible for reduced serum levels of T4 and progesterone. Treatment with gossypol was responsible for a significant reduction in the number of normal ovarian follicles and a significant increase in the number of atretic follicles, both in all stages of development. Thus, treatment of rats with gossypol was responsible for reduction in the number of viable follicles and changes in hormone levels that resulted in interference of the estrous cycle.

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Histological and hormonal studies of calabash chalk on ovarian function in adult female wistar rats

Journal of Morphological Sciences ◽

10.4322/jms.114317 ◽

2017 ◽

Vol 34 (03) ◽

pp. 173-177

Author(s):

A. Oyewopo ◽

K. Obasi ◽

K. Anumudu ◽

E. Yawson

Keyword(s):

Body Weight ◽

Adult Female ◽

Wistar Rats ◽

Ovarian Function ◽

Female Rats ◽

Control Group ◽

Treatment Groups ◽

Female Wistar Rats ◽

Group A ◽

Group B

Abstract Introduction: Calabash chalk is a naturally occurring mineral, chiefly composed of fossilized sea shells. It is prepared from clay and mud mixed with other ingredients, including lead, arsenic, sand and wood ash. Clay consumption is correlated with pregnancy, and also to eliminate morning sickness in women. The aim of this study is to evaluate the effects of calabash chalk on the ovarian function in adult female Wistar rats. Methods: Eighteen (18) adult female Wistar rats. Group A served as the control group, group B received 40 mg/kg body weight of Non-salted calabash chalk while group C received 40 mg/kg body weight of Salted calabash chalk for 14 days. On day 15, the animals were sacrificed for histological and biochemical examination. Results: Results showed a significant (P< 0.05) reduction in follicle stimulating hormone (FSH) levels of the treatment groups when compared with the control group and an increase in luteinizing hormone (LH) levels of the treatment groups when compared with the control group. Histological examination of the ovaries showed severe deterioration of the ovarian follicles, necrosis and follicular atresia. Conclusion: Calabash chalk is toxic to ovarian function. These alterations have been shown to be the leading cause ofinfertility in female rats. Hence, proper monitoring, education, and regulation of the product is needed.

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