scholarly journals Ethanolic extract of black grapes (Vitis Vinifera) ameliorates overtraining-induced pancreatic β-cells and muscle damage

2020 ◽  
Author(s):  
Ferbian Milas Siswanto ◽  
Risha Catra Pradhany ◽  
Boedi Prihatini Yenniastoeti ◽  
Alex Pangkahila
Keyword(s):  
Vitis Vinifera ◽  
Muscle Damage ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
High Volume ◽  
Control Group ◽  
Albino Rats ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Control Group Design

Overtraining is a high-volume, high-intensity, long-duration, and high-frequency training. The heavier physical training, the more oxygen needed for metabolism. Increasing oxygen usage causes an elevation of electron leakage in mitochondria;thus,produce a higher amount of reactive oxygen species (ROS). Antioxidant inhibits oxidative damage in a target molecule. Grapes contain a lot of antioxidants, such as polyphenols and anthocyanins. The purpose of this study was to examine the effect of ethanol extract of Balinese grapes (Vitis vinifera) on β-cells and muscle damage in overtraining-induced rats. This study was a completely randomized experimental study using a posttest only control group design. Samples were 36 male albino rats (Rattus norvergicus), aged 2.5-3 months, divided randomly into two groups. The control group (P0), 18 rats, were given overtraining and placebo of 2 ml distilled water; the treatment group (P1) 18 rats, were given overtraining and 25g/kg.BW ethanol extract of Balinese grapes. The result showed the average amount of pancreatic β-cells in the P0 group was higher than the P1 group (p< 0.001). In addition, the average level of creatine kinase was also higher in the P1 group than those of the P0 group (p<0,001). It can be concluded that the administration of ethanolic extract of Balinese grapes mitigates the damage on pancreatic β-cells and muscle cells induced with overtraining.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

2020 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Viani Anggi ◽  
Joni Tandi ◽  
Veronika Veronika
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Total Flavonoid ◽  
Male Rats ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
White Rats ◽  
Group I ◽  
Streptozotocin Induced Diabetes

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.  

scholarly journals The Effect of Co-Administration of Telang Leaves Ethanolic Extract Towards Fluoxetin’s Sedative Effect on Male Balb/C Mice Based on Sleeping Duration Parameter

2017 ◽  
Vol 6 (1) ◽  
pp. 7
Author(s):  
Anif Nur Artanti ◽  
Maria Ulfah
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sedative Effect ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Control Group Design ◽  
Group Design ◽  
Sleep Analysis ◽  
Post Test ◽  
The Mean

One of Telang plant’s advantages is its activity as sedative agent. Previous studies indicate that Telang plant has anticonvulsant activity on mice at certain dose. This study was conducted to find out the sedative effect of Telang plant, particularly its leaf, when being co-administered with fluoxetin. Phytochemical analysis was carried out qualitatively on Telang ethanolic extract leaves (TEE) to find out the content of chemical compound first.This study was an experimental research with post-test only control group design, employing male balb/c mice. The effect of Telang ethanolic extract co-administration with fluoxetin was observed. The parameter being used in the study was the duration of sleep. Analysis was done by comparing mice sleeping duration prior to administration of fluoxetin alone and in combination with Telang ethanolic extract. The data was then analyzed using SPSS 17.0 for Windows.The results showed that TEE contained tannin, saponin, and flavonoid compounds, and co-administration of TEE and fluoxetin at various doses could provide sedative effect on mice. The co-administration of 400 mg/kgBW extract and 15 mg/kgBW fluoxetin could provide the mean sleeping duration 43 minutes longer than positive control.Keywords:  telang leaves ethanol extract, fluoxetin, sleeping duration

scholarly journals No Acute Toxicity Tests of Purwoceng (Pimpinella pruatjan Molk.) Ethanolic Extract on Male Albino Rat by Determined Hepatorenal Function Test and Histopathology

Molekul ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 117
Author(s):  
Fitranto Arjadi ◽  
Dhadhang Wahyu Kurniawan ◽  
Yudhi Wibowo ◽  
Wahyu Siswandari ◽  
Lantip Rujito
Keyword(s):  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Toxicity Tests ◽  
Control Group ◽  
Acute Administration ◽  
Albino Rat ◽  
Control Group Design ◽  
Post Test ◽  
Liver And Kidney ◽  
Group B

Pimpinella pruatjan Molkis a local indegineuos plant speciescommonly used  aphrodisiac.This studywas performed to analyze the effect of acute administration of purwoceng (Pimpinella pruatjan Molk.) roots ethanol extract to liver and kidney histological damagein rats. The study used an experimental approachusing post test only with control group design. Rats were randomly divided into five groups; 3 rats in each group. Group A as a control group received aquadest, group B, C, D, and E were given purwoceng roots ethanol extract dose of 5 mg/kgBW, 50 mg/kgBW, 300 mg/kgBW, and 2000 mg/kgBW respectively.Liver histological damage was assessed by a modification of the Roenigk score, whereaskidney damage was by the semiquantitative scoring of proximal tubular necrosis. UV test was used to quantify the AST and ALT levels, the measurement of blood urea levels was using the Urease-GLDH method, and Jaffe methodwas used to access the creatinine levels.Kruskal-Wallis test showed that liver and kidney histologicalparameterswere not significantly affected, as well as theblood urea and creatinine levels (p>0.05).Meanwhile,ALT level wasonly parameters which showed the significant test (p <0.05)among groups. Study concluded that the liver and kidney histological appearance, AST, blood urea, and creatinine levels in the male albino rat were not significantly affected by acute administration of Purwoceng roots in various dosesbut  the ALT level was significantly affected

Hepatoprotective Role of Propolis Extract to Prevent Hepatotoxic Effects of ATT in patients with Tuberculosis

2021 ◽  
Vol 15 (9) ◽  
pp. 2867-2869
Author(s):  
Asma Arshad ◽  
Saira Munawar ◽  
Rabia Sajjad Toor ◽  
Saba Saleem ◽  
Kanwal Sharif ◽  
...  
Keyword(s):  
Standard Dose ◽  
Ethanolic Extract ◽  
Control Group ◽  
Albino Rats ◽  
P Value ◽  
Average Weight ◽  
Propolis Extract ◽  
Measured Weight ◽  
Group B ◽  
Group D

Objective: The main purpose of this study is to evaluate the hepatoprotective effects of propolis in hepatocytes injury caused by ATT due to isoniazid and rifampicin. Methods: Healthy albino rats of with average weight of 200-250g were under this study. These rats dividing into main four groups, A group is taken a control group and then further into the group B, group C, and group D as group for experiments. The control group had 15 rats with measured weight, they were given distilled water. Group B had 15 rats, they were given with standard dose of rifampicin and isoniazid. Group c had 15 rats, they were also given with standard dose of rifampicin and isoniazid. Group D had 15 rats, they were given with standard dose of rifampicin and isoniazid and also extract of the propolis we prepared. Results: Serum ALT in the experimental group B with group C, group D were also found to be of statistically significant with p-value < 0.001. ALT serum level observed high in group B. Multiple comparison between groups revealed that group B with a significantly increase in the serum enzyme AST level in comparison to group A, group C and group D with p-value <0.001. Conclusion: This study showed that ethanolic extract of propolis prevents isoniazid and rifampicin induced hepatotoxicity in the albino rats. Key words; Propolis, Anti-tuberculosis treatment, Hepato-toxicity.

scholarly journals Subacute Toxicity Effect of Psidium guajava Leaves Ethanolic Extract on Gaster in Wistar Rat

2018 ◽  
Vol 16 (1) ◽  
pp. 61
Author(s):  
Esti Dyah Utami ◽  
Hanif Nasiatul Baroroh ◽  
Nuryanti Nuryanti
Keyword(s):  
Toxic Effect ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Psidium Guajava ◽  
Albino Rats ◽  
Respiratory Disorder ◽  
Subchronic Toxicity ◽  
Toxicity Effect ◽  
Test Materials

Psidium guajava leaves have been proven be antiallergic. From the results of previous research, the ethanol extract of leaves is included in the class materials "Practically Non-Toxic." However, repeated administration may cause toxic effects on body organs, one of which was gaster. Subchronic toxicity had been carried out on 20 male Wistar rats and 20 female wistar albino rats. The test materials were administered orally, every day for 28 days. There are 3 (three) doses of test materials which were 250 mg, 500 mg, and 750 mg/kg BW. Observation of the general toxic effect was performed everyday, then organ sampling was performed on day 29 to examined gaster histology and score using Barthel-Manja methods. The result showed that administration of Psidium guajava leaves extract for 28 days caused physical and behavioral changes, caused soft and mucous feces, and also respiratory disorder. But the extract at dose 250 mg, 500 mg and 750 mg/kg BW did not caused changes on gaster histology profile and score. In conclusion, the extract did not have subchronic toxicity effect on gaster rat, but might caused general toxic effect in the higher dose.

scholarly journals The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

2017 ◽  
Vol 15 (2) ◽  
pp. 196
Author(s):  
Much Ilham Novalisa Aji Wibowo ◽  
Nur Aeni ◽  
Zidna Mazayatul Huda ◽  
Nunuk Aries Nurulita
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Group Iv ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Male Wistar Rats ◽  
Hepatoprotective Agent ◽  
Better Than

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

scholarly journals Effectiveness of Antidiarrheal Unripe Wooden Banana (Musa paradisiaca L.) in Male Balb-C/Mice Induced with Escherichia coli

2020 ◽  
Vol 56 (3) ◽  
pp. 208
Author(s):  
Arista Wahyu Ningsih ◽  
Maftuchah Rochmanti ◽  
Achmad Basori
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Negative Control ◽  
Morbidity Rate ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Musa Paradisiaca ◽  
Fecal Weight ◽  
Antidiarrheal Activity

The survey results in Indonesia in 2017 showed that the diarrhea morbidity rate for all age groups was 6.897.463. In Indonesia, unripe wooden banana has been used empirically as an antidiarrheal by the people in Senduro village, Lumajang, East Java. The study aimed to prove the antidiarrheal effect of ethanol extract of unripe wooden banana (Musa paradisiaca L.) in male Balb-C/mice induced by Escherichia coli bacteria. This study was a laboratory experimental study with post-test only control group design that used 40 mice divided into 8 groups, namely 1 group without treatment (normal mice) and 7 groups induced by Escherichia coli (1 negative control group given CMC-Na, 3 groups positive controls were given Loperamid HCL 0,5 mg/kgBW, 1 mg/kgBW and 2 mg/kgBW and 3 treatment groups were given extracts 100 mg/kgBW, 200 mg/kgBW and 400 mg/kgBW). Observation of animals in this study was carried out for 4 hours. Data were analyzed of frequency of diarrhea, fecal weight and fecal consistency used Kruskal Wallis and the results showed significant differences between treatment groups (p <0.05). From the results of the Mann-Whitney test, the ethanol extract of unripe wooden banana with a dose of 100 mg/kgBW was not significantly different (p> 0.05) with the control group of Loperamid HCL dose of 0.5 mg/kgBW. Unripe wooden banana had been shown to have antidiarrheal activity at an extract dose of 100 mg/kg BW in terms of the parameters of fecal consistency, frequency of diarrhea, and fecal weight. The results showed that the higher the dose, the better antidiarrheal activity.

scholarly journals BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (4) ◽  
pp. 257
Author(s):  
Mutiara Indah Sari ◽  
Maya Anjelir Antika ◽  
Dwi Rita Anggraini
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Wistar Rat ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Control Group Design ◽  
Glucose Levels

 Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

Ethanol Extract Combinations Effect of Celery Herb (Apium graveolens L.) and Bay Leaf (Syzygium polyanthum W.) Toward Hypertensive Mice Induced by Sodium Chloride and High Fat Feed

2021 ◽  
Vol 1162 ◽  
pp. 151-158
Author(s):  
Moch. Saiful Bachri ◽  
Widyasari Putranti ◽  
Lina Widiyastuti ◽  
Resalianti Sintiana Devie
Keyword(s):  
Blood Pressure ◽  
Sodium Chloride ◽  
Research Result ◽  
Ethanol Extract ◽  
Apium Graveolens ◽  
Control Group ◽  
High Fat ◽  
Control Group Design ◽  
Group Design ◽  
Hypertension Therapy

The combination of herb medicine is alternative option in hypertension because it has more potential for treatment with complications like hyperlipida. Plant which can be used for anti-hypertension therapy is combination of celery herb (Apium graveolens) and bay leaf ethanol extract (Syzygium polyanthum). This research aims to determine the activity of a combination of celery herb ethanol extract (CHEE) and bay leaf ethanol extract (BLEE) and find out how much the decrease of blood pressure on the combination of both toward hypertension with high fat Wistar mice. The design of this research used an experimental design with pre-post control group design. Hypertensive mice are induced with high fat feed and orally with sodium chloride 8%, then the mice are supplied with combinations extract with the dose of 1.125 ; 6.25 ; 2.25 ; 12.5 and 4.5 ; 25 mg/kg, hydrochlorotiazide 2.25 mg/kg, Simvastatin 0.9 mg/kg, CHEE 4.5 mg/kg, BLEE 25 mg/kg and CMC-Na 0.5%. Research result shows that the combination can decrease systole blood pressure in the 22nd day. The extract combination has anti-hypertension effect (it is able to decrease systole blood pressure ≥ 20 mmHg) and it is not significantly different with normal group (p<0.05). Based on the research, it can be concluded that ethanol extract combination can decrease systole blood pressure with high fat complications after using it for 22 days.

Sign in / Sign up

Export Citation Format

Share Document