scholarly journals Production of Endoglucanases by Streptomyces thermocoprophilus CP1 using Rice Straw as a Substrate

2021 ◽  
Author(s):  
Suriya Tingthong ◽  
Pitchayaporn Suwanakood ◽  
Pongsak Rattanachaikunsopon ◽  
Jidapa Sangswan
Keyword(s):  
Phylogenetic Analysis ◽  
Rice Straw ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Agar Plate ◽  
Alternative Substrate ◽  
Cmcase Activity ◽  
Semi Solid ◽  
Alignment Analysis ◽  
Optimal Ph

Rice straw is a major agricultural waste that can be used as an alternative substrate to expensive raw materials for endoglucanases (CMCase) production by microorganisms. This study aimed to search for a microorganism having the potential to produce endoglucanase from rice straw. From compost samples, 40 bacterial colonies were isolated on carboxymethylcellulose (CMC) agar. Among them, 16 isolates showed a hydrolysis zone on a CMC agar plate with hydrolysis (HC) values ranging from 1.15±0.02 to 4.40±0.52. Based on hydrolysis zone diameter and HC value, isolates CP1, CP2 and CP3 were further examined for their CMCase production in CMC broth. According to CMCase production and stability, isolate CP1 was selected for further study. The optimal pH and temperature for CMCase production of isolate CP1 were 5 and 45 °C, respectively. When using pre-treated rice straw as a substrate for semi-solid-state fermentation, the highest CMCase activity of 0.142 ± 0.008 U/mL was obtained in a medium containing pre-treated rice straw of 60 g/L. The sequence alignment analysis and phylogenetic analysis suggested that the isolate CP1 was likely to be Streptomyces thermocoprophilus. The microorganism obtained from this study may be not only industrially important but also beneficial to the environment.

scholarly journals Bioteknologi Fermentasi Jerami Padi Tinggi Nutrisi, Guna Meningkatkan Kemandirian dan Kesejahteraan Peternak di Desa Kalibendo

2021 ◽  
Vol 2 (1) ◽  
pp. 23-32
Author(s):  
Roni Yulianto ◽  
Nurwidodo Nurwidodo ◽  
Desy Cahya Widianingrum ◽  
Himmatul Khasanah
Keyword(s):  
Rice Straw ◽  
Animal Feed ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Educational Institutions ◽  
Feed Processing ◽  
Long Period ◽  
Fermented Feed ◽  
Village Government

Abstrak: Kabupaten Lumajang memiliki lahan pertanian dan perkebunan dengan komoditi utamanya adalah tanaman padi, jagung dan kopi. Banyak sekali limbah yang belum termanfaatkan, berawal dari program KKN, potensi daerah di Desa Kalibendo, Kecamatan Pasirian, Kabupaten Lumajang sangat potensial untuk dikembangkan salah satunya “Bioteknologi fermentasi jerami padi tinggi nutrisi, guna meningkatkan kemandirian dan kesejahteraan peternak di Desa Kalibendo, Kecamatan Pasiria, Kabupaten Lumajang”. Pendampingan dari instansi terkait edukasi dalam bioteknologi pengolahan pakan berkualitas secara mandiri dari bahan baku lokal yang ada dari limbah pertanian setempat dapat memenuhi ketersediaan pakan ternak baik dari segi kualitas maupun kuantitas. Pemanfaatan Limbah jerami padi menggunakan bioteknologi fermentasi untuk pakan ternak ruminansia (sapi, kambing/domba) perlu terus dikembangkan, karena dengan fermentasi dapat meningkatkan kualitas nutrisi pakan ternak, dan memanfatkan limbah hasil pertanian menjadi berdaya guna sehingga tidak terbuang begitu saja, dan bisa digunakan dalam jangka waktu yang lama untuk ketersediaan pakan ternak pada saat musim kemarau atau disaat hijauan sudah mulai berkurang. Target dari Program Pengabdian Desa Binaan yaitu bersama dengan pemerintah Desa Kalibendo melakukan pemberdayaan petani-peternak dalam pengolahan pakan ternak fermentasi, sehingga dapat mewujudkan kemandirian dan kesejahteraan peternak.Abstract: Lumajang Regency has extensive agricultural and plantation land, especially rice, corn, and coffee crops, a lot of waste that has not been utilized. Starting from the KKN program, regional potential in Kalibendo Village, Pasirian District, Lumajang Regency is very potential to be developed, one of which is Biotechnology for fermented rice straw with high nutrition, to increase the independence and welfare of breeders in Kalibendo village, Pasiria district, Lumajang Regency. Assistance from educational institutions related to quality feed processing biotechnology independently from existing local raw materials from local agricultural waste can meet animal feed availability in terms of quality and quantity. Utilization of rice straw waste uses biotechnology for ruminant animal feed (cows, goats/sheep), it needs to be continuously developed because fermentation can improve the nutritional quality of animal feed, and utilize agricultural waste to be efficient so that it is not wasted, and can be used in a long period for the availability of fodder during the dry season when the forage has diminished. The Assisted Village Service Program's target is to collaborate with the Kalibendo Village government to empower farmers in the processing of fermented feed so that they can realize the independence and welfare of the breeders.

scholarly journals Valorization of rice straw waste: production of porcelain tiles

Cerâmica ◽  
2015 ◽  
Vol 61 (360) ◽  
pp. 442-449 ◽  
Author(s):  
Álvaro Guzmán A ◽  
Silvio Delvasto A ◽  
Maria Francisca Quereda V ◽  
Enrique Sánchez V
Keyword(s):  
Rice Straw ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Research Work ◽  
Clay Content ◽  
Environmental Benefits ◽  
Raw Material ◽  
Waste Production ◽  
Porcelain Tiles ◽  
Porcelain Tile

Abstract The rice industry generates huge amounts of rice straw ashes (RSA). This paper presents the results of an experimental research work about the incorporation of RSA waste as a new alternative raw material for production of porcelain tiles. The RSA replaces, partially or completely, the non-plastic raw materials (quartz (feldspathic sand in this research) and feldspar), that together with the clays, constitute the major constituents of formulations of porcelain tiles. A standard industrial composition (0% RSA) and two more compositions in which feldspar and feldspathic sand were replaced with two percentages of RSA (12.5% RSA and 60% RSA) were formulated, keeping the clay content constant. The mixtures were processed, reproducing industrial porcelain tile manufacturing conditions by the dry route and fired at peak temperatures varying from 1140-1260 ºC. The results showed that additions of 12.5% RSA in replacement of feldspar and feldspathic sand allowed producing porcelain tiles that did not display marked changes in processing behaviour, in addition to obtain a microstructure and the typical mineralogical phases of porcelain tile. Thus, an alternative use of an agricultural waste material is proposed, which can be translated into economic and environmental benefits.

Utilization of rice straw and tea composts for strawberry and tomato production

2020 ◽  
Vol 30 (4) ◽  
pp. 335-343
Author(s):  
R Ashrafi ◽  
RM Saiem ◽  
M Kamruzzaman ◽  
MSAA Mamun ◽  
HA Begum
Keyword(s):  
Rice Straw ◽  
Agricultural Waste ◽  
Total Sugar ◽  
Fruit Yield ◽  
Compost Tea ◽  
Tomato Production ◽  
Horticultural Crops ◽  
Beneficial Effects ◽  
Combined Application ◽  
Yield And Quality

The use of agricultural waste is of great interest to sustainable agriculture. An investigation was carried out to evaluate the effects of compost and compost tea made from agricultural waste rice straw on the yield and quality of two horticultural crops strawberry and tomato. In strawberry experiment, six treatments were considered which wereT1: 100% soil (as a control), T2: 80% soil + 20% compost, T3: 60% soil + 40% compost, T4: 40% soil + 60% compost, T5: 20% soil + 80% compost & T6: 100% compost. Results revealed that number of fruit, fruit yield and total sugar (%)was increased with the increasing level of compost up to 80% after that decreased at 100% compost. The treatment T5: 20% soil + 80% compost gave the best results among the treatments to grow strawberry with good yield (185.3 g/plant) and sweetness (total sugar 5.19%). On the other hand, six treatments i.e. T1: Control, T2: Compost (5 t/ha), T3: Compost tea (50% concentration), T4: Compost (5 t/ha) + Compost tea (50% concentration), T5: Compost tea (100% concentration) and T6: Compost (5t/ha) + Compost tea (100% concentration) were considered in tomato experiment. As a result, it was observed that combined application of compost (5 t/ha) along with compost tea (100% conc.) (T6) gave the highest yield of tomato than not only control (T1) but also single application of compost or compost tea (T2, T3, T4&T5). Level of compost tea concentration also showed significant effect on fruit yield of tomato. Comparing between treatment T4 and T6, fruit yield was found higher (1027.67 g/plant) in treatment T6: Compost (5t/ha) + Compost tea (100% conc.) than 961.3 g/plant in T4: Compost (5 t/ha) + Compost tea (50% conc.). So, it could be summarized that use of rice straw as compost and compost tea affects positively both in two experiments. The results of this study confirm the beneficial effects of compost to increase the yield and sweetness of strawberry and combination of compost and compost tea to increase the yield of tomato. Progressive Agriculture 30 (4): 335-343, 2019

Agricultural waste as a source of innovative and compostable composite biopolymers for food packaging: a scientific review

2021 ◽  
Author(s):  
Anna Ronzano ◽  
Roberta Stefanini ◽  
Giulia Borghesi ◽  
Giuseppe Vignali
Keyword(s):  
Food Packaging ◽  
Agricultural Land ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Natural Fibers ◽  
Scientific Review ◽  
Food And Agriculture ◽  
Food And Agriculture Organization ◽  
Regional Project ◽  
Materials Used

"The recovery of agriculture waste is one of the challenges of 2030 Agenda. Food and Agriculture Organization states that 30 % of the world’s agricultural land is used to produce food that is later lost or wasted, and the global carbon footprint corresponds to 7% of total greenhouse gases emissions. Alternatively, natural fibers contained in food and agricultural waste could be a valuable feedstock to reinforce composite biopolymers contributing to increase mechanical properties. In addition, the use of biopolymers matrix could contribute significantly to reduce the environmental footprint of the biobased compounds. Based on these premises, a regional project in Emilia-Romagna, aims to enhance agricultural waste to produce food packaging materials which in turn would contribute to the reduction of green raw materials used. This article reviews the state of art of composite biopolymers added with fillers extracted by food and agricultural waste, analyzing the literature published on scientific databases such as Scopus. The characteristics, advantages and drawbacks of each innovative sustainable material will be studied, trying to compare their various properties. The results of the work could guide companies in the choice of eco-sustainable packaging and lay the foundations for the development of the mentioned regional project."

Research of pneumatic conveyor for overloading of fuel pellets

2021 ◽  
pp. 88-96
Author(s):  
N. Spodyniuk ◽  
◽  
L. Horbachenko ◽  
Keyword(s):  
Key Words ◽  
Agricultural Sector ◽  
Raw Materials ◽  
Agricultural Waste ◽  
High Quality ◽  
Fuel Pellets ◽  
Energy Consuming ◽  
Loading And Unloading

The constant development of the country's agricultural sector allows the production of biofuels, such as fuel pellets, from agricultural waste - straw, corn and sunflower husks. However, the transportation of fuel pellets is quite energy consuming. The process of loading and unloading, transportation over long distances requires complex mechanized equipment. The use of a pneumatic conveyor for grain reloading will allow to provide high-quality transportation of fuel pellets. The aim of the article was to investigate the operation of the pneumatic conveyor for overloading fuel pellets, to determine the optimal indicators that affect the productivity of the pneumatic conveyor. Since fuel pellets, as raw materials, are structurally similar to cereals, a study of the pneumatic grain conveyor PTZ-25 was conducted. The dependence of the productivity of the pneumatic conveyor on the lifting height h, m and the length of the pipelines L, m was obtained. The obtained results showed that by reducing the length of the pipelines by four times and the lifting height by half, the productivity of the pneumatic conveyor will increase by 1.15 times. Key words: fuel pellets, pneumatic conveyor, productivity

scholarly journals New methods of acid hydrolysis of cellulose and plant raw materials

2021 ◽  
Vol 57 (1) ◽  
pp. 119-128
Author(s):  
V. S. Boltovsky
Keyword(s):  
Acid Hydrolysis ◽  
Plant Biomass ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Feed Additives ◽  
Process Conditions ◽  
Catalytic Systems ◽  
Plant Raw Materials ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Prospects for the development of hydrolysis production are determined by the relevance of industrial use of plant biomass to replace the declining reserves of fossil organic raw materials and increasing demand for ethanol, especially for its use as automobile fuel, protein-containing feed additives that compensate for protein deficiency in feed production, and other products. Based on the review of the research results presented in the scientific literature, the analysis of modern methods of liquid-phase acid hydrolysis of cellulose and various types of plant raw materials, including those that differ from traditional ones, is performed. The main directions of increasing its efficiency through the use of new catalytic systems and process conditions are identified. It is shown that the most promising methods for obtaining monosaccharides in hydrolytic processing of cellulose and microcrystalline cellulose, pentosan-containing agricultural waste and wood, are methods for carrying out the process at elevated and supercritical temperatures (high-temperature hydrolysis), the use of new types of solid-acid catalysts and ionic liquids. 

scholarly journals PEMBUATAN PUPUK ORGANIK DARI KOTORAN SAPI DAN JERAMI PADI DENGAN PROSES FERMENTASI MENGGUNAKAN BIOAKTIVATOR M-DEC

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Ahmad Shobib
Keyword(s):  
Rice Straw ◽  
Fermentation Process ◽  
Raw Materials ◽  
Organic Fertilizer ◽  
Organic Matter Content ◽  
Organic Content ◽  
Matter Content ◽  
Cow Manure ◽  
Aerobic Fermentation ◽  
Fermentation Time

Cow manure can be used for making organic fertilizer because it contains nutrients such as Nitrogen (N), Phosphorus (P), and Potassium (K). Rice straw has a high C-Organic content. Adding straw compost will increase soil organic matter content. The study aims to determine the fermentation process that occurs so as to produce organic fertilize, know the effect of fermentation time and the effect of the composition of raw materials for cow manure and rice straw on the process of making organic fertilizer to the content of organic fertilizer according to SNI 7603 : 2018. The method use by aerobic fermentation is by mixing cow manure and rice straw and M-Dec bioactivators by comparison 3 : 1, 2 : 2,1 : 3 and fermentation time 7, 14, 21, 28 days. The parameters tested are C-organic, Nitrogen (N), C/N ratio, Phosphorus (P), and Potassium (K). The best quality organic fertilizer in the treatment of cow manure : rice straw with a ratio of 2 : 2 on the 28th day fermentation process namely C-organic content 34,63 %, C/N ratio is 25, macro nutrient content N+P2O5+K2O of 3,14 % that has met SNI 7763 : 2018. Keyword: M-Dec bioactivator, aerobic fermentation, cow manure, rice straw, C/N ratio

scholarly journals A novel high performance metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

2020 ◽  
Author(s):  
Shohreh Ariaeenejad ◽  
Atefeh Sheykhabdolahzadeh ◽  
Morteza Maleki ◽  
Kaveh Kavousi ◽  
Mehdi Foroozandeh Shahraki ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Specific Activity ◽  
Agricultural Waste ◽  
Extreme Temperature ◽  
In Silico Analysis ◽  
Cellulose Content ◽  
Biomass Hydrolysis ◽  
Harsh Condition

Abstract Background: Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a wide search for novel effective enzymes to improve the exploitation of lignocellulose, among which the importance of thermostable and halotolerant cellulase enzymes with high pH performance is significant. Results: The primary aim of this study was to discover a novel alkali-thermostable endo-β-1,4-glucanase from the sheep rumen metagenome. Using a multi-step in-silico analysis, primary candidates with desired properties were found and subjected to cloning, expression, and purification followed by functional and structural characterization. The enzymes' kinetic parameters, including V max , Km, and specific activity, were calculated. The PersiCel4 demonstrated its optimum activity at pH 8.5 and a temperature of 85°C and was able to retain more than 70% of its activity after 150 hours of storage at 85°C. Furthermore, this enzyme was able to maintain its catalytic activity in the presence of different concentrations of NaCl, MgCl 2 , CaCl 2 , and MnCl 2 . Our results showed that treatment with MnCl 2 could enhance the enzyme’s activity by 89%. PersiCel4 was ultimately used for enzymatic hydrolysis of autoclave pretreated rice straw, the most abundant agricultural waste with rich cellulose content. In autoclave treated rice straw, enzymatic hydrolysis with the PersiCel4 increased the release of reducing sugar up to 260% after 72 hours in the harsh condition ( T= 85°C, pH = 8.5). Conclusion: Considering the urgent demand for stable cellulases that are operational on extreme temperature and pH conditions and due to several proposed distinctive characteristics of PersiCel4, it can be used in the harsh condition for bioconversion of lignocellulosic biomass.

scholarly journals WOOD COMPONENTS AS SOURCES OF PENTO-CONTAINING RAW MATERIALS FOR SYNTHESIS OF USEFUL COMPOUNDS, PRODUCTS AND REAGENTS

2021 ◽  
Vol 64 (3) ◽  
pp. 107-115
Author(s):  
Irina A. Chetvertneva ◽  
Oleg Kh. Karimov ◽  
Galina A. Teptereva ◽  
Natalia S. Tivas ◽  
Eldar M. Movsumzade ◽  
...  
Keyword(s):  
Raw Materials ◽  
Condensation Reaction ◽  
Agricultural Waste ◽  
Monomer Unit ◽  
Paper Industry ◽  
Raw Material ◽  
Chemical Transformations ◽  
Covalent Bonds ◽  
Industry Waste ◽  
Wood Processing

The paper considers the main components and products of wood processing, agricultural waste, pulp and paper industry waste and qualifies them as sources of pentose-containing resource-renewable domestic raw materials. The article describes in detail the structural components of wood as a natural polymer, which contains aromatic and carbohydrate parts. It is noted that these poly-mers are promising as raw materials for the production of useful chemical products. The role of lignin, cellolose and hemicellulose in the design of mechanical and structural properties of wood is considered. The article considers the features of the sulfonation reactions of the lignin monomer unit depending on the pH of the medium: acidic, neutral and alkaline. There are three main reac-tions that occur simultaneously with lignin in the process of wood delignification during sulfite cooking, such as the sulfonation reaction, the hydrolytic destruction reaction, and the condensation reaction. It is shown that the lignin-hemicellulose matrix contains three types of interconnected mesh structures: the lignin itself; a network of covalent bonds of lignin with hemicelluloses, and a network whose structure is obtained due to the hydrogen bond and the forces of the physical inter-action of lignin and hemicelluloses. The features of chemical transformations of the monomeric aromatic link of lignosulfonate – phenylpropane unit in the processes of wood delignification, the main chemical reactions of wood raw material delignification under the conditions of sulfite and neutral-sulfite brews are shown. The method of quantitative determination of monosaccharides in the composition of the carbohydrate part is proposed.

scholarly journals Pemanfaatan Pelepah Sawit dan Kotoran Ayam Ras untuk Pembuatan Pupuk Bokashi pada Kelompok Wanita Tani Padang Baru Babatan, Seluma, Bengkulu

2021 ◽  
Vol 3 (1) ◽  
pp. 16
Author(s):  
Nurlianti Nurlianti ◽  
Prihanani Prihanani
Keyword(s):  
Oil Palm ◽  
Broiler Chicken ◽  
Family Income ◽  
Raw Materials ◽  
Agricultural Waste ◽  
Chicken Manure ◽  
Lecture Demonstration ◽  
Farmer Groups ◽  
Chicken Waste ◽  
Palm Fronds

The Utilization of Oil Palm Midrib-leaf and Chicken Manure for Making Bokashi Fertilizer in the Women's Farmers Group Padang Baru Babatan, Seluma, Bengkulu The purpose of the community service program is to utilize agricultural waste, namely oil palm midrib-leaf and chicken manure which are often found in this Babatan Village, Sukaraja Subdistrict, Seluma Regency, Bengkulu. In addition, this program was aimed to increase knowledge and skill to produce bokashi fertilizer with raw materials found around them. The methods used were lecture, demonstration plots, and mentoring. The results of the activity were the increasing of family income by making bokashi utilizing waste raw materials around their lives, namely bokashi made from palm fronds and broiler chicken waste that is ready to be sold. In limited quantities, they have been able to sell their products to meet the needs of other farmer groups or the general public in need.

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