scholarly journals Relation of R-Spondin 3 Gene expression android obesity and Susceptibility to Cardiovascular disease in Sudanese Patients in Khartoum State.

2019 ◽  
Vol 9 (2) ◽  
pp. 355-361
Author(s):  
Ashraf M.A. Alkinain ◽  
Kamal Eldin Ahmed Abdelsalam ◽  
Mutaz Ibrahim Hassan
Keyword(s):  
Gene Expression ◽  
Cardiovascular Disease ◽  
Abdominal Obesity ◽  
Cardiometabolic Risk ◽  
Negative Control ◽  
Obese Group ◽  
Control Group ◽  
Obese Patients ◽  
Abdominal Adiposity ◽  
Heart Group

Background: Obesity is a major risk factor for the development of cardiovascular disease. A growing database of clinical evidence implicates intra-abdominal adiposity as a powerful driving force for elevated cardiometabolic risk. Addressing intra-abdominal adiposity should play a central role in future strategies aimed at improving cardiovascular outcomes in patients with abdominal obesity and its associated cardiometabolic risk in Sudan. Several studies aimed to identify some factors controlling the size and function of different areas of fat. Our research is focusing on a particular gene called R-SPONDIN3, Objectives: It is to find the amount of R-Spondin3 Gene expression in Abdominal obesity and Susceptibility to Cardiovascular disease in Sudanese Patients in Khartoum State Material and methods: - The study was including 300 participants (156 males and 144 females) classified into three groups. The first group was including one hundred participants with abdominal obesity (obese), the second group was including one hundred participants already diagnosed with CVD entangled with obesity (Heart Group as positive control group), while the third group was include one hundred healthy lean volunteers (negative control group) Results: - The findings of this study showed Conventional PCR results were significantly different (P <0.001) in Heart group subjects as compared to healthy controls and obese group. Among heart group mutation was detected in some subjects (19%) and the rest without mutation (81%) but in obese group no mutation was detected. Comparison between the different studied groups according to gene expression showed significant differences (P <0.001) mean value of gene expression in healthy group subjects was 1.0 ± 0.0, Obesity group was 2.44 ± 0.50 and heart group subjects was 4.54 ± 0.87 respectively.  Conclusion: The amount of R-SPONDIN3 gene expression among the obese and CVD patients is show up significant different and the amount of gene expressing among the CVD patients is higher than obese which is suggested that the amount of gene expressed in obese patients with heart disease more than obese patients without cardiovascular complications  

scholarly journals Role of Roof plate-specific SPONDIN3 Mutation in the Determination of Obesity Phenotypes/Fat Distribution and Susceptibility to Cardiovascular disease in Sudanese Patients in Khartoum State

2019 ◽  
Vol 9 (2) ◽  
pp. 118-124
Author(s):  
Ashraf M.A. Alkinain ◽  
Kamal Eldin Ahmed Abdelsalam ◽  
Mutaz Ibrahim Hassan
Keyword(s):  
Gene Expression ◽  
Cardiovascular Disease ◽  
Abdominal Obesity ◽  
Cardiometabolic Risk ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Abdominal Adiposity ◽  
Conventional Pcr ◽  
Heart Group

Background: Obesity is a major risk factor for the development of cardiovascular disease. A growing database of clinical evidence implicates intra-abdominal adiposity as a powerful driving force for elevated cardiometabolic risk (1). Addressing intra-abdominal adiposity should play a central role in future strategies aimed at improving cardiovascular outcomes in patients with abdominal obesity and its associated cardiometabolic risk in Sudan. Objectives: It is to find the mutation in R-SPONDIN3gene and its association to both of fat deposition around the abdomen and susceptibility to cardiovascular disease in Sudanese patients in Khartoum State. Material and methods: Conventional PCR was done to detect R- SPONDIN3 in 300 participants (males and females) classified into three groups. The first group will include one hundred participants with abdominal obesity, the second group will include one hundred participants already diagnosed with CVD entangled with obesity (positive control group), while the third group will include one hundred healthy lean volunteers (negative control group). Data was analyzed using SPSS Version 22 software. P value < 0.05 was considered as statistically significant. Results: In this study, the results of Conventional PCR were significantly different in (P <0.001) in Heart group subjects as compared to healthy controls and obese group. Comparison between the different studied groups according to gene expression showed significant differences (P <0.001) mean value of gene expression in healthy group subjects was 1.0 ± 0.0, Obesity group was 2.44 ± 0.50 and heart group subjects was 4.54 ± 0.87 respectively . Conclusion: clinically, detect R- SPONDIN3 mutation in patients with diagnosed with CVD entangled with obesity and amount of the gene expressed cleared different between obese and CVD subjects entangled with obesity. Keywords: R-SPONDIN3 gene, abdominal Obesity, CVD.

The influence of obesity on functional outcome and quality of life after total knee arthroplasty

2018 ◽  
Vol 100-B (5) ◽  
pp. 579-583 ◽  
Author(s):  
S. Xu ◽  
J. Y. Chen ◽  
N. N. Lo ◽  
S. L. Chia ◽  
D. K. J. Tay ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Total Knee Arthroplasty ◽  
Functional Outcome ◽  
Knee Arthroplasty ◽  
Obese Group ◽  
Control Group ◽  
Obese Patients ◽  
Knee Score ◽  
Total Knee

AimsThis study investigated the influence of body mass index (BMI) on patients’ function and quality of life ten years after total knee arthroplasty (TKA).Patients and MethodsA total of 126 patients who underwent unilateral TKA in 2006 were prospectively included in this retrospective study. They were categorized into two groups based on BMI: < 30 kg/m2(control) and ≥ 30 kg/m2(obese). Functional outcome was assessed using the Knee Society Function Score (KSFS), Knee Society Knee Score (KSKS), and Oxford Knee Score (OKS). Quality of life was assessed using the Physical (PCS) and Mental Component Scores (MCS) of the 36-Item Short-Form Health Survey.ResultsPatients in the obese group underwent TKA at a younger age (mean, 63.0 years, sd 8.0) compared with the control group (mean, 65.6 years, sd 7.6; p = 0.03). Preoperatively, both groups had comparable functional and quality-of-life scores. Ten years postoperatively, the control group had significantly higher OKS and MCS compared with the obese group (OKS, mean 18 (sd 5) vs mean 22 (sd 10), p = 0.03; MCS, mean 56 (sd 10) vs mean 50 (sd 11), p = 0.01). After applying multiple linear regression with the various outcomes scores as dependent variables and age, gender, and Charlson Comorbidity Index as independent variables, there was a clear association between obesity and poorer outcome in KSFS, OKS, and MCS at ten years postoperatively (p < 0.01 in both KSFS and OKS, and p = 0.03 in MCS). Both groups had a high satisfaction rate (97.8% in the control group vs 87.9% in the obese group, p = 0.11) and fulfillment of expectations at ten years (98.9% in the control group vs 100% in the obese group, p = 0.32).ConclusionAlthough both obese and non-obese patients have significant improvements in function and quality of life postoperatively, obese patients tend to have smaller improvements in the OKS and MCS ten years postoperatively. It is important to counsel patients on the importance of weight management to achieve a more sustained outcome after TKA. Cite this article: Bone Joint J 2018;100-B:579–83.

scholarly journals Effects of siRNA-mediated silencing of Bmi-1 gene expression on proliferation of gastric cancer cells

2019 ◽  
Vol 17 ◽  
pp. 205873921984553
Author(s):  
Ying Guo ◽  
Li Zhang ◽  
Guangyu Zhou ◽  
Qingjie Ma ◽  
Shi Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Flow Cytometry ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Negative Control ◽  
Control Group ◽  
Gastric Cancer Cells ◽  
Ags Cells

This study was designed to investigate the effects of siRNA-mediated silencing of Bmi-1 gene expression on proliferation of AGS gastric cancer cell. siRNA Bmi-1 was transfected into human AGS gastric cancer cells by liposome (as siRNA Bmi-1 group) with negative control (as control group); the expressions of Bmi-1 and apoptosis-related genes like P21, Bax, and Bcl-2 in AGS cells were determined by Western blot method; the apoptosis of AGS cells was detected by flow cytometry double staining and Hoechst staining; and cell cycle was measured by flow cytometry. Compared with the control group, the expression of Bmi-1 in the siRNA Bmi-1 group was significantly decreased ( P < 0.05), the apoptosis rate was increased ( P < 0.05), and cell cycles were arrested at G1 phase (P < 0.05); the expression level of P21 and Bax in cells was significantly up-regulated while that of Bcl-2 down-regulated ( P < 0.05). The down regulation of Bmi-1 can inhibit the proliferation of AGS gastric cancer cell and promote its apoptosis, which takes such effects mainly by up-regulating P21 as well as Bax and down-regulating Bcl-2.

scholarly journals Intra-abdominal adiposity, abdominal obesity, and cardiometabolic risk

2008 ◽  
Vol 10 (suppl_B) ◽  
pp. B4-B10 ◽  
Author(s):  
Ele Ferrannini ◽  
Anna Maria Sironi ◽  
Patricia Iozzo ◽  
Amalia Gastaldelli
Keyword(s):  
Abdominal Obesity ◽  
Cardiometabolic Risk ◽  
Abdominal Adiposity

scholarly journals Interleukin gene expression in broiler chickens infected by different Escherichia coli serotypes

2021 ◽  
pp. 2727-2734
Author(s):  
Reham Elnagar ◽  
Rasha Elkenany ◽  
Gamal Younis
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Broiler Chickens ◽  
Quantitative Polymerase Chain Reaction ◽  
Clinical Signs ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
E Coli ◽  
High Prevalence

Background and Aim: Escherichia coli is the cause of avian colibacillosis, a significant threat to the poultry industry and public health. Thus, this study investigated the prevalence of E. coli in diseased chicken broilers, pathological effects of these bacteria, and interleukin (IL) gene expression of different serotypes of E. coli (O78, O26, O44, and O55) on experimentally infected chickens. Materials and Methods: A total of 295 organ samples (liver, lungs, heart, and spleen) from 59 diseased broiler chickens were used for conventional identification of E. coli. Chickens were orally infected with one of the following E. coli serotypes (O78, O26, O44, or O55) and examined for clinical signs, mortality, macroscopic and microscopic lesions, and IL gene expression using real-time quantitative polymerase chain reaction. Results: E. coli was isolated from 53.2% of broiler chicken organs with a high prevalence in lungs (26.1%). The most prevalent serotypes were O78, O26, O44, O55, O157, and O127 prevalence of 27.8, 22.2, 16.7, 16.7, 5.6, and 5.6%, respectively. In the experimental design, five groups (G1-G5) of birds were established. G1 served as the negative control group, while G2-G5 were challenged orally with E. coli O78, O26, O55, or O44, respectively. Chickens infected with E. coli O78 or O26 showed significant clinical signs in comparison to the other infected birds. Mortality (13.3%) was only observed in birds infected with E. coli O78. Necropsy of dead birds after E. coli O78 infection showed pericarditis, enteritis, airsacculitis, and liver and lung congestion. More severe histopathological changes were observed in intestines, spleen, liver, and lung from chickens infected with either E. coli O78 or O26 than for birds infected with other serotypes. On the 2nd day post-infection, E. coli challenge, particularly with E. coli O78, displayed significantly upregulated levels of ileal IL-6 and IL-8, but ileal IL-10 level tended to be downregulated in comparison to the control group. Conclusion: This study assessed the application of cytokines as therapeutic agents against infectious diseases, particularly colibacillosis.

scholarly journals Antihiperlipidemi and Atheroprotective activity of Kepuh (Sterculia foetida) Leaves Ethanolic Extract on High-Fat-Diet Rat Models

2017 ◽  
Vol 3 (1) ◽  
pp. 44
Author(s):  
Ika Rahmawati Sutejo ◽  
Imama Rasyada ◽  
Annafira Yuniar
Keyword(s):  
Cardiovascular Disease ◽  
High Fat Diet ◽  
Foam Cell ◽  
Ethanolic Extract ◽  
Foam Cells ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aortic Tissue ◽  
High Fat

Cardiovascular disease causes the highest mortality rate in the world. Atherosclerotic plaque is the most common etiology of cardiovascular disease. High levels of cholesterol in circulation is the pathophysiology of atherosclerosis formation. Atherosclerosis risk can be lowered to 20-40% with statins. Statins are effective in lowering total and LDL cholesterol, but these drugs have been reported to cause side effects. Therefore, it needed an alternative medicine to prevent the process of atherosclerosis using kepuh leaves (Sterculia foetida). Flavonoids contained in Kepuh leaves improve lipid profiles. The aims of this study is to prove the effectiveness of Sterculia foetida in lowering cholesterol, triglyceride and reduce the amount of foam cells on high-fat-diet induced rats. The research design is quasi-experimental with post test only design. Animals are grouped into normal group, negative control group and three treatment groups that were given kepuh leaves extract at the dose of 200 mg/kgBB, 400 mg/kgBB, and 800 mg/ kgBB. Rats were given high-fat diet of initial intravenous adrenaline injection 0.006 mg/200 gBB and duck egg yolks 5 g/kgBB for 21 days. Cholesterol and triglycerides were measured by enzymatic methods and aortic tissue stained with HE. ESf reduce cholesterol significantly and reduce the amount of foam cells on aorta.Keywords: cholesterol, triglyceride, kepuh leaves, foam cell, high-fat-diet

scholarly journals The chemerin production changes in obese patients with different carbohydrate metabolism state

2017 ◽  
Vol 63 (6) ◽  
pp. 582-590 ◽  
Author(s):  
M.A. Vasilenko ◽  
E.V. Kirienkova ◽  
D.A. Skuratovskaya ◽  
P.A. Zatolokin ◽  
N.I. Mironyuk ◽  
...  
Keyword(s):  
Gene Expression ◽  
Type 2 Diabetes ◽  
Adipose Tissue ◽  
Mrna Expression ◽  
Carbohydrate Metabolism ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Obese Patients ◽  
Tissue Specific

Chemerin is a mediator of adipose tissue involved in the regulation of many processes, including lipogenesis, and inflammatory response. The role of chemerin in the development of insulin resistance has been insufficiently studied and needs detailed understanding. The aim of the study was to investigate chemerin production in obese patients with different states of carbohydrate metabolism. The study included 155 patients with a diagnosis of obesity; 34 patients with overweight. The control group 1 consisted of 43 conditionally healthy donors who did not have obesity. For comparison of the results of a study to determine the levels of tissue-specific mRNA expression of the genes IL-6, TNF-a, RARRES2, (encoding IL-6, TNF-a and chemerin) in adipose tissue introduced a control group 2 – 30 patients without obesity. Study on the relative level of mRNA expression of the genes IL-6, TNF-a and RARRES2 (encoding IL-6, TNF-a and chemerin) was carried out using real time PCR. Concentrations of IL-6, TNF-a, and chemerin were measured in serum/plasma using an enzyme-linked immunosorbent assay (ELISA). We found significant differences in the plasma level of chemerin and tissue-specific features of RARRES2 gene expression in obese patients, depending on the degree of obesity and the state of carbohydrate metabolism. Multidirectional associations of RARRES2 gene expression with TNF-a and IL-6 genes in adipose tissues of different localization are shown: in obese patients (BMI £40 kg/m2) without type 2 diabetes – negative, and type 2 diabetes – positive. Identified relationship chemerin plasma content and the expression level of its gene in biopsies with various parameters of carbohydrate and lipid metabolism, proinflammatory molecules indicate chemerin involved in metabolic and immune processes in obesity.

scholarly journals Antiplatelet Effectivity between Aspirin with Honey on Cardiovascular Disease Based on Bleeding Time Taken on Mice

2019 ◽  
Vol 7 (20) ◽  
pp. 3416-3420
Author(s):  
Sake Juli Martina ◽  
Logaselvi A. P. Ramar ◽  
Michael R. I. Silaban ◽  
Muhammad Luthfi ◽  
Pradeepa A. P. Govindan
Keyword(s):  
Cardiovascular Disease ◽  
Bleeding Time ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Post Test ◽  
The Mean ◽  
The University ◽  
North Sumatra ◽  
Aspirin Group

BACKGROUND: The prevalence of cardiovascular disease (CVD) and its mortality continues to increase. Various studies have shown aspirin can reduce CVD mortality but has adverse side effects. Research on a comparison between aspirin and honey has not been done, but both have antiplatelet effects. AIM: This study is aimed to prove the antiplatelet effects on honey and compare the antiplatelet effects of aspirin with honey based on the bleeding time in mice. METHODS: This study is a true experimental design with a post-test only control group using 32 male mice, Double Ditsch Webster, ± 3 months old, the weight of 20-30 g, divided into 4 groups. Consisting of a negative control group (placebo), aspirin and honey. The suspension has given orally for 12 days using the probe. The research was conducted at the Laboratory of Pharmacology Department of Pharmacology and Therapeutics Faculty of Medicine, the University of North Sumatra in September until December 2015. The data collected was bleeding time in mice. Data analysed by Shapiro Wilk test, Kruskal Wallis and Mann Whitney. RESULTS: The mean bleeding time was a placebo (102.88 seconds), aspirin (369.38 seconds) and honey (304.63 seconds). Mann Whitney test showed significant results in the aspirin and honey groups against the control group (placebo) with p = 0.001. There were no significant differences in the aspirin group against honey (p = 0.172). Honey has an antiplatelet effect in mice. The mean bleeding time in mice given honey is longer or closer to the mean bleeding time in the aspirin group. CONCLUSION: The results could be used as a basis for further research to determine its use in humans with cardiovascular disease.

Peripheral nerve dysfunction and its correlation to interleukin-6 level in obese patients

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mary Fayez Francis ◽  
Mona Mahmoud Arafa ◽  
Irene Raouf Amin ◽  
Manal Samier Ahmad
Keyword(s):  
Peripheral Nerve ◽  
Median Nerve ◽  
Ulnar Nerve ◽  
Interleukin 6 ◽  
Nerve Conduction ◽  
Obese Group ◽  
Control Group ◽  
Obese Patients ◽  
Posterior Tibial ◽  
Significant Difference

Abstract Background Obesity is now a worldwide health problem that predisposes to development of numerous complications, one of which is peripheral neuropathy (PN). Elevated interleukin-6 (IL-6) cytokine levels associating the chronic inflammatory state in obesity is thought to play a major role in obesity complications. Methods We conducted a Cross-sectional study on 30 obese patients and 10 matched healthy lean controls. All participants were subjected to full history taking, neurological examination, motor and sensory peripheral nerve conduction studies (NCS). Serum IL-6 levels were assessed using a commercially available ELISA. Laboratory investigations such as glycated haemoglobin, thyroid profile, liver and kidney function tests were done to exclude other known causes of PN. Results Radial, Posterior Tibial and Peroneal nerves had statistically significantly lower motor amplitudes in obese groups compared to control group. Sensory amplitudes of Median nerve showed statistically significant lower values in obese group with PN compared to control group. Conduction velocities (CV) of Ulnar nerve (motor and sensory) and sensory Median nerve showed statistically significant lower CVs in the obese group with PN compared to obese group without PN. Motor Ulnar nerve CV showed highly statistically significant higher velocities in the obese group without PN compared to control group. Posterior Tibial nerve distal motor latencies and Sensory nerve peak latencies of Median nerve had statistically significant higher values in obese group with PN compared to obese group without PN while statistically significant lower sensory peak latencies of Radial nerve were found in obese group without PN compared to control group. Regarding serum IL-6 levels, highly statistically significant higher levels were found in either obese groups when compared to the control group while no statistical significant difference was present between both obese groups and no correlation was found between serum IL-6 and NCS parameters in obese group with PN. Conclusion Our study revealed that despite only 6.667% of our non diabetic obese patients complained of symptoms of PN, by NCS 66.67% of patients were found to have electrophysiological evidence of PN while the remaining 33.33% of patients showed no evidence of PN and no correlation was found between serum IL-6 levels and nerve conduction parameters changes in obese patients diagnosed with PN.

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