scholarly journals PENGARUH GEL EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav) TERHADAP PENYEMBUHAN LUKA BAKAR PADA KELINCI (Oryctolagus cuniculus)

2016 ◽  
Vol 2 (2) ◽  
pp. 103-110
Author(s):  
Fina Ulviani ◽  
Yusriadi Yusriadi ◽  
Khildah Khaerati
Keyword(s):  
Oryctolagus Cuniculus ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Metal Plate ◽  
Hot Metal ◽  
Burn Wounds ◽  
Treatment Groups ◽  
Betel Leaf ◽  
Ability Tests

Research on Gel Formulation of red betel leaf extract (Piper crocatum Ruiz & Pav) for the treatment of burn wounds in rabbits (Oryctolagus cuniculus) has been conducted. This study aimed to determine variation’s effect of concentration in gel of red betel leaf extract for the treatment of burn wounds in rabbits which had been wounded using hot metal. In this study, the viscous extract was formulated into gel with concentrations of 1%, 2% and 3%. The Gel’s Physical quality evaluation included organoleptic, pH, homogeneity and dispersive ability tests on day 0 until day 28. The gel activity test was performed on 5 rabbits divided into five treatment groups. Each rabbit was burn-wounded using hot metal plate at diameter of 20 mm. Each group was given five wounds consisting of a negative control, 1%, 2%, 3% of extract formula and a positive control. The diameter measurement of the wounds was done on the 1st, 3rd, 5th, 7th, 9th and the 21st day. Data of wound healing percentage was statistically analyzed with Two-Way ANOVA. The results showed that the gel with 3% of red betel leaf extract of which percentage of healing as much as 85.81% compared to gel extract 1% and 2% with percentage 65,32% dan 76,58%.

scholarly journals Analgesic effectiveness comparison between red betel leaf extract (Piper crocatum) and therapy dosage of aspirin in mice

2017 ◽  
Vol 11 (1) ◽  
pp. 1-6
Author(s):  
DIAN AJENG ATIKANINGRUM ◽  
ENDANG EDININGSIH ◽  
CR. SITI UTARI
Keyword(s):  
Treatment Group ◽  
Analgesic Effect ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Betel Leaf ◽  
Significant Difference

Atikaningrum DA, Ediningsih E, Utari CRS. 2013. Analgesic effectiveness comparison between red betel leaf extract (Piper crocatum) and therapy dosage of aspirin in mice. Biofarmasi 11: 1-6. The aim of this research was to find out an analgesic effectiveness of red betel leaf extract (Piper crocatum) compared to the therapy dosage of aspirin in mice. This research used a completely randomize experimental design. The subject in this research were 30 male mice of Swiss which in age of 2-3 months with 20-30 grams weight. The subjects were divided into five treatment groups: (i) negative control group (aquadest), (ii) positive control group (aspirin), (iii) first treatment group (3.64 mg red betel leaf extract), (iv) second treatment group (7.28 mg red betel leaf extract), and (v) third treatment group (14.56 mg red betel leaf extract). Analgesic effect was determined by counting the mice jump on 42oC in hotplate during 5 minutes, 2 hours after treatment. The obtained data were tested statistically by Anova and Pos-Hoc processed by Lead Significance Difference. The Anova test showed that there were significant differences among five treatment groups, while the LSD test showed that there were significant difference between negative control group and treatment groups, and the second and third treatment groups showed no significant difference with positive control group. The red betel leaf extract had an analgesic effect when it was given orally in mice. The treatment groups that had the same efficacy with the aspirin treatment group were the second (7.28 mg red betel leaf extract) and the third groups (14.56 mg red betle leaf extract).

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals Uji Efektivitas Ekstrak Etanol Daun Angsana (Pterocarpus Indicus Willd) sebagai Biolarvasida terhadap Larva Nyamuk Culex Sp.

2021 ◽  
Vol 1 (1) ◽  
pp. 33-37
Author(s):  
Mohammad Ihsan Abdurrozak ◽  
Livia Syafnir ◽  
Esti Rachmawati Sadiyah
Keyword(s):  
Human Health ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Probit Analysis ◽  
Mosquito Larvae ◽  
Treatment Groups ◽  
Lc50 Value ◽  
Probit Method ◽  
Culex Sp

Abstract. Mosquitoes are often associated with health problems because mosquito bites not only cause itching but some species can also transmit various types of parasites that are harmful to human health. One of them is Culex sp mosquito which is a class of infectious insects (vectors). This study aimed to test the activity of compounds in angsana leaf extract (Pterocarpus indicus Willd) as biolarvasides on Culex sp. mosquitoes and determine the concentration of LC50 value needed. Extraction was carried out by maceration method using 70% ethanol solvent. The extract collected was then tested biolarvaside activity on Culex sp. The study subjects were divided into 8 treatment groups, namely aquades (negative control), 0.05%, 0.1%, 0.2%, 0.5%, 1%, 2% and ABATE 0.1% (positive control). Each group contained 20 mosquito larvae with three replications (triplo). Observations were made for 24 hours at intervals of 1 hour, 4 hours, 8 hours, 12 hours and 24 hours. The biolarvaside activity of angsana leaf extract was analyzed using the probit method. Based on the results of the Probit analysis, the LC50 value was 0.83%. These results indicate that the angsana leaf extract (Pterocarpus indicus Willd) was effective as a biolarvaside. Abstrak. Nyamuk sering dikaitkan dengan masalah kesehatan karena gigitan nyamuk tidak hanya menimbulkan gatal saja tetapi beberapa spesies nyamuk juga dapat menularkan berbagai jenis parasit yang berbahaya bagi kesehatan manusia. Salah satunya yaitu nyamuk Culex sp yang merupakan golongan serangga penular (vektor). Penelitian ini bertujuan untuk pengujian aktivitas senyawa dalam ekstrak daun angsana (Pterocarpus indicus Willd) sebagai biolarvasida pada nyamuk Culex sp dan penentuan nilai LC50. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, Ekstrak yang diperoleh kemudian diuji aktivitas biolarvasida pada nyamuk Culex sp. Subjek penelitian dibagi menjadi 8 kelompok perlakuan, yaitu akuades (kontrol negatif), 0,05%, 0,1%, 0,2%, 0,5%, 1%, 2% dan ABATE 0,1% (kontrol positif).Setiap kelompok berisi 20 ekor larva nyamuk dengan tiga kali pengulangan (triplo) . Pengamatan dilakukan selama 24 jam dengan interval 1jam, 4jam, 8jam, 12jam dan 24jam. Aktivitas biolarvasida ekstrak daun angsana dianalisis dengan menggunakan metode probit. Berdasarkan hasil analisis Probit didapatkan nilai LC50 berada pada konsentrasi 0,83%.Hasil tersebut menunjukkan bahwa Ekstrak daun angsana (Pterocarpus indicus Willd) efektif sebagai biolarvasida.

scholarly journals Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) terhadap Struktur Histologis Pankreas Tikus Putih (Rattus norvegicus L.) Hiperglikemia

2021 ◽  
Vol 6 (2) ◽  
pp. 115-123
Author(s):  
Nunuk Shofiati ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Histological Structure ◽  
Distilled Water ◽  
Treatment Groups ◽  
Randomized Design ◽  
Neem Leaves ◽  
Neem Leaf Extract

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok  tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans. 

scholarly journals The Addition of Red Betel Leaf Extract (Piper crocatum) in the Feed of Vannamei Shrimps (Litopenaeus vannamei) for Vibriosis Prevention

2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fariq Azhar ◽  
Muhammad Junaidi ◽  
Bagus Dwi Hari Setyono ◽  
Andre Rachmat
Keyword(s):  
Survival Rate ◽  
Bacterial Infection ◽  
Litopenaeus Vannamei ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Betel Leaf ◽  
Shrimp Feed ◽  
Number Of Bacteria ◽  
Total Bacteria

Vibriosis is a type of shrimp disease caused by Vibrio sp. In the disease control of consumption biota, it is highly recommended to use natural ingredients that are not carcinogenic, one of which is red betel leaves (Piper crocatum). This study aims to determine the best dose of red betel leaf extract (P. crocatum) mixed in Vannamei shrimp feed to prevent vibriosis. In this study, shrimps were reared for 40 days at a density of 20 fish/container. The treatments included positive control, P1 (without extract + bacterial infection), negative control P2 (without extract and without bacterial infection), P3 (0.5% extract + bacterial infection), P4 (1% extract + infection), and P5 (2% extract + bacterial infection). The application of red betel leaf extract at a dose of 0.5% resulted in 75% survival after infection with V. parahaemolyticus, Vannamei shrimps of THC 7.70×106 cells/mL, and DHC (hyaline 82.94% granular 20.10%). The number of bacteria and the number of vibrio in the intestine were 52×108 CFU/mL and 12×108 CFU/mL accordingly. The best dose was obtained at P3 (Feed +0.5% red betel leaf extract), seen from the increase in survival rate, the number of hemocytes, differential haemocyte counts, and a decrease in total bacteria, so the application of red betel leaf extract can be used in the cultivation of Vannamei shrimps as an immunostimulant.

PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

BioScience ◽  
2018 ◽  
Vol 2 (1) ◽  
pp. 61
Author(s):  
Elsa Yuniarti
Keyword(s):  
Blood Glucose ◽  
Mus Musculus ◽  
Leaf Extract ◽  
Negative Control ◽  
State University ◽  
Betel Leaf ◽  
Randomized Design ◽  
The Subject ◽  
Effective Drugs ◽  
Zoology Department

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

2019 ◽  
Vol 1 (2) ◽  
pp. 11-16
Author(s):  
Yanna Rotua Sihombing ◽  
Debi Dinha Sitepu
Keyword(s):  
Treatment Group ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno®  32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno®  32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

Sign in / Sign up

Export Citation Format

Share Document