Spinal Structure of Mice Fetus (Mus musculus L.) Treated by Acanthus ilicifolius L. Leaves Extract

Jeruju had various chemical components. This study was conducted on the teratogenic test of jeruju leaf extract to determine abnormalities in fetal mice (Mus musculus L.). This study used a completely randomized design (CRD) method with 20 pregnant female mice divided into 4 groups: 1 group as a control and 3 groups treated with a dose of 3.75 mg/30grBB, 7.5 mg/30grBB, 15 mg/grBB, each with jeruju leaf extract consists of 5 repetitions. The results showed that the doses of 7.5 mg/30grBB and 15 mg/grBB significantly decreased fetal weight and length when compared with controls. This is suspected because there is an active substance in the jeruju leaf extract which is cytotoxic. Anatomically the administration of jeruju leaf extract does not cause abnormalities in the structure of the fetal spine of mice. This is due to the growing fetus taking priority from the many types of nutrients in the mother fluid so that the fetal bone can continue to grow and develop.

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Pengaruh Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Yang Terpapar Asap Rokok.

BIOSAINTROPIS (BIOSCIENCE-TROPIC) ◽

10.33474/e-jbst.v7i1.445 ◽

2021 ◽

Vol 7 (1) ◽

pp. 74-84

Author(s):

I Komang Alit Saputra ◽

Ni Gusti Ayu Manik Ermayanti ◽

A.A. S. A. Sukmaningsih

Keyword(s):

Cigarette Smoke ◽

Mus Musculus ◽

Leaf Extract ◽

Coffea Canephora ◽

Robusta Coffee ◽

Randomized Design ◽

Completely Randomized Design ◽

One Way Anova

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.

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STRUKTUR HISTOLOGI HATI MENCIT(Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN EKOR NAGA (Rhapidhophora pinnata Schott)

SIMBIOSIS Journal of Biological Sciences ◽

10.24843/jsimbiosis.2017.v05.i02.p02 ◽

2017 ◽

Vol 5 (2) ◽

pp. 43

Author(s):

Ni Komang Tia Pramesti ◽

Ngurah Intan Wiratmini ◽

Ni Putu AdrianiAstiti

Keyword(s):

Oral Administration ◽

Mus Musculus ◽

Leaf Extract ◽

Fatty Degeneration ◽

Histopathological Changes ◽

Treatment Groups ◽

Randomized Design ◽

Treatment Of Hypertension ◽

Medical Plant ◽

Completely Randomized Design

ABSTRACT Rhapidhophora pinnata, Schott is a traditional medical plant that has been use remedy for treatment of hypertension, stroke. Aims of this research is to determine the effect of Rhapidhophora pinnata Schott leaf extract toward mouse (Mus musculus L) liverhistological structure. This research used completely randomized design with 28 female mice that were divide into 4 groups of P0 (control) received 0,9% NaCl, group P1, P2, and P3 received 50, 100 and 150 mg/kg bw respectively by oral administration. The treatment were given daily for 14 days. All of the mice were sacrificed by ether after 15 days. The liver were examined for their histopathological changes, namely fatty degeneration, hydropic degenaration and necrosis. Results were statistically analised by Kruskal-Wallis method. Treatment groups showed no significant differences with regard of liver histopatological changes, however hemorrhage, sinusoid congestion and inflamatory cell infiltration were found in liver. Keyword : Rhapidhophora pinnata Schott, liver, mice

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PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

EKSAKTA Berkala Ilmiah Bidang MIPA ◽

10.24036/eksakta/vol19-iss1/124 ◽

2018 ◽

Vol 19 (1) ◽

pp. 43-55

Author(s):

Ramadhan Sumarmin

Keyword(s):

Blood Glucose ◽

Mus Musculus ◽

Leaf Extract ◽

Negative Control ◽

State University ◽

Betel Leaf ◽

Randomized Design ◽

The Subject ◽

Completely Randomized Design ◽

Zoology Department

Patients withdiabetes mellitus(DM) continues to grow becauseprosperityandpeople's lifestyles.Treatment of diabetesoften useinjectionsof insulinandoralantidiabeticdrugs. Thetreatmenthas no side effects. Therefore, it isnecessary to findeffective drugsusing plantsthatred betelleaf(Piper crocatumRuiz&Pav.). Red betel leafcontains flavonoidswhichare antioxidants. This study aimsto determine the effectand dose ofextractof redbetel leaf(Piper crocatumRuiz&Pav.) The mosteffectiveagainstbloodglucosein mice(Mus musculusL.) maleinducedsucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

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PENGARUH EKSTRAK DAUN SIRIH MERAH (Piper crocatum Ruiz & Pav.) TERHADAP GLUKOSA DARAH MENCIT (Mus musculus L.) JANTAN YANG DIINDUKSI SUKROSA

BioScience ◽

10.24036/02018219965-0-00 ◽

2018 ◽

Vol 2 (1) ◽

pp. 61

Author(s):

Elsa Yuniarti

Keyword(s):

Blood Glucose ◽

Mus Musculus ◽

Leaf Extract ◽

Negative Control ◽

State University ◽

Betel Leaf ◽

Randomized Design ◽

The Subject ◽

Effective Drugs ◽

Zoology Department

Patients withdiabetes mellitus (DM) continues to grow because prosperity and people's lifestyles.Treatment of diabetes often usei njections of insulin and oral antidiabetic drugs. Thetreatment has no side effects. Therefore, it is necessary to find effective drugs using plants thatred betel leaf (Piper crocatum Ruiz & Pav.). Red betel leaf contains flavonoids which are antioxidants. This study aims to determine the effect and dose of extract of red betel leaf (Piper crocatum Ruiz & Pav.) The most effective agains blood glucose in mice(Mus musculusL.) male induced sucrose.This study was an experimental study. The research was conducted in October 2015 in the Division of Laboratory Animal and Zoology Department of Biology, State University of Padang. The subject of research in the form of mice (Mus musculus L.) males totaled 24 tails. The design used was completely randomized design (CRD) with 6 treatments and 4 repetitions. The treatment is given as follows: treatment I: the diabetes control without any treatment given, treatment II: as a negative control (sucrose 3 g/kg bw), treatment III: sucrose+suspension of red betel leaf extract (dosage 0,7 g/kg bw), treatment IV: sucrose+suspension of red betel leaf extract (dosage 1,4 g/kg bw), treatment V: sucrose+suspension of red betel leaf extract (dosage of 2,1 g/kg bw) and treatment VI: sucrose+suspension extracts red betel leaf (dosage 2,8 g/kg bw).The results showed that the extract of red betel leaf (Piper crocatum Ruiz & Pav.) at a dose of 0,8 g/kg bw 1,4 g/kg bw 2,1 g/kg bw and 2,8 g/kg bw can lowers blood glucose in mice. However, the most appropriate dose in lowering blood glucose in mice (Mus musculus L.) at 2,8 g/kg bw in mice.

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PENGARUH EKSTRAK DAUN MIMBA TERHADAP PERKEMBANGAN HAMA Callosobruchus chinensis L. (Coleoptera : Bruchidae)

AGRICA ◽

10.37478/agr.v1i2.306 ◽

2019 ◽

Vol 1 (2) ◽

pp. 32-37

Author(s):

Yustina M.S.W Puu ◽

Hildegardis Nalti Nansi

Keyword(s):

Mung Bean ◽

Leaf Extract ◽

Callosobruchus Chinensis ◽

Spawning Activity ◽

Green Beans ◽

Leaf Extracts ◽

Randomized Design ◽

Completely Randomized Design ◽

Contact Poison ◽

Neem Leaf Extract

Callosobruchus Chinensis is a pest that damages mung bean seeds in storage and causes damage to both the quality and quantity of seeds. This study aims to determine the effectiveness of neem leaf extracts in suppressing the development of the Callosobruchus Chinensis pest as one of the postharvest pests in the commodity green beans. This research conducted at the Laboratory of the Faculty of Agriculture, University of Flores, by using a Completely Randomized Design (CRD) with five treatments and three replications. The concentration of neem leaf extract treatment is 0 ml / l, 25 ml / l, 30 ml / l, 35 ml / l, and 40 ml / l. The results showed that the concentration of neem leaf extract 40 ml / l caused mortality of C. Chinensis imago as contact poison by 99% and nerve poison by 47%. While the effectiveness of the limb leaf extract on spawning activity was 86%.

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PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus

el–Hayah ◽

10.18860/elha.v5i4.3476 ◽

2016 ◽

Vol 5 (4) ◽

pp. 169

Author(s):

Sri Puji Astuti Wahyuningsih ◽

Virid Gibson ◽

Alfiah Hayati

Keyword(s):

Molecular Weight ◽

Treatment Group ◽

Mus Musculus ◽

Control Group ◽

Protein Profiles ◽

Testosterone Levels ◽

Randomized Design ◽

Sds Page ◽

Elisa Technique ◽

Completely Randomized Design

<em>The purpose of this research was to determine the effect of polysaccharide krestin</em> (<em>PSK) </em><em>on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of 15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of 60 mg/kgBW had lowest level at dosage, i.e. 25946.8 ρg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.</em>

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Potensi Ekstrak Karamunting (Melastoma malabathricum L.) sebagai Insektisida Nabati untuk Mengendalikan Ulat Grayak (Spodoptera litura F.)

Agrotekma Jurnal Agroteknologi dan Ilmu Pertanian ◽

10.31289/agr.v4i1.2806 ◽

2019 ◽

Vol 4 (1) ◽

pp. 28

Author(s):

Kartina Kartina ◽

Shulkipli Shulkipli ◽

Mardhiana Mardhiana ◽

Saat Egra

Keyword(s):

Spodoptera Litura ◽

Leaf Extract ◽

Feeding Activity ◽

Larval Mortality ◽

Wild Plant ◽

Botanical Insecticides ◽

Botanical Insecticide ◽

Melastoma Malabathricum ◽

Randomized Design ◽

Completely Randomized Design

<h1>Spodoptera litura F. is one of the important pests in Horticulture commodities which can cause damage 80% and up to 100%. Karamunting (Melastome malabathricum) is a wild plant that grows in Tarakan City. This plant is known to contain secondary metabolites which are thought to have the potential to control pests. This study aims to determine the potential of Karamunting extract as a botanical insecticide material to control armyworms. This study uses a completely randomized design (CRD) method with 1 treatment factor, namely the concentration of Karamunting leaf extract. Potential extracts as botanical insecticides were tested by dye method using 4 concentrations, namely 0 (control); 500; 1000; 2000; 4000; and 8000 ppm. The results showed that Karamunting leaf extract was able to control armyworm pests. Giving extracts of 8000 ppm (P5) in feed caused the highest decrease in eating activity up to 41,2%, larval mortality up to 85%, and the highest mortality rate after 7 days of observation. Decreased feeding activity and larval mortality were thought to be influenced by the presence of phenol compounds, fatty acids, terpenoids, sterols and alkaloids identified in the extract. Thus, Karamunting leaf extract has the potential to be developed as an active ingredient in botanical insecticides to control armyworm.<strong></strong></h1>

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PENGARUH WAKTU MASERASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban)

Jurnal Ilmu dan Teknologi Pangan (ITEPA) ◽

10.24843/itepa.2020.v09.i04.p12 ◽

2020 ◽

Vol 9 (4) ◽

pp. 482

Author(s):

Ni Komang Trisna Rahayu ◽

I Dewa Gede Mayun Permana ◽

GA. Kadek Diah Puspawati

Keyword(s):

Antioxidant Activity ◽

Experimental Design ◽

Leaf Extract ◽

Centella Asiatica ◽

Total Flavonoid ◽

Time Duration ◽

Time Treatment ◽

Randomized Design ◽

Completely Randomized Design ◽

Effect On Yield

This research aimed to determine the effect of maceration time on the antioxidant activity of pegagan leaf extract and to determine the best maceration time with highest antioxidant activity of pegagan leaf extract. The experimental design used was Completely Randomized Design with maceration time duration there were 18, 24, 30, 36, 42, and 48 hours. All of the treatment repeated three times to obtained 18 units of experiments. The data analyzed by analyze of variance, if the treatment had a significant effect followed by The Duncan Test. The results showed that the maceration time treatment had a significant effect on yield, total phenolik, total flavonoid, total tannin and antioxidant activity of pegagan leaf extract. The 24 hour maceration time was the best treatment with antioxidant activity 66.67%, IC50 632.82 ppm, a yield of 24.30%, a total phenolik of 57.85 mgGAE / g, a total flavonoid of 105.28 mgQE / g. total tannin 54.09 mgTAE / g.

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KAJIAN SIFAT ORGANOLEPTIK DAN DAYA TERIMA ES KRIM JALOR (JAMBU BIJI DAN SARI DAUN KELOR)

Jurnal Gizi Prima ◽

10.32807/jgp.v4i1.128 ◽

2019 ◽

Vol 4 (1) ◽

pp. 47

Author(s):

Nadya Natasya

Keyword(s):

Adolescent Girls ◽

Experimental Research ◽

Young Women ◽

Leaf Extract ◽

Ice Cream ◽

Food Product ◽

Acceptance Test ◽

Randomized Design ◽

Completely Randomized Design ◽

Moringa Leaf Extract

Background: Young women generally have characteristics of unhealthy meal habits and many of them lack of nutrients needed, so there is a need to have an alternative nutritionally rich and attractive food product through the manufacture of JALOR Ice Cream which is produced from Milk, Guava and Moringa Leaf Extract, to increase iron in order to prevent iron deficiency.Methods: This study was conducted using an experimental research design. The experiment was carried out using a Completely Randomized Design (CRD) using 5 treatments with the addition of Moringa Leaf Extract: t1 (10%), t2 (20%), t3 (30%), t4 (40%) and t5 (50%). The experiment were carried out on hedonic tests on 20 panelists with 5 scales, namely: 5 = Very like, 4 = Like, 3 = Neutral, 2 = Dislike, and 1 = Very dislike. Acceptance test was conducted on young women.Result: The results of the organoleptic test showed that JALOR Ice Cream was chosen on treatment t2 with the addition of 20% Moringa Leaf Extract. The results of the adolescent girls' acceptance of selected JALOR Ice Cream products showed that all (100%) panelists were at the level of good category.Conclusion: JALOR Ice Cream is with the addition of 20% Moringa Leaf Extract is the most preffered. The acceptance of Ice Cream can be well received by panelists.

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Bioactivity of Torch Ginger Umbut Extract (Etlingera elatior) Against Heal Wounds of Mice (Mus musculus)

EKSAKTA Berkala Ilmiah Bidang MIPA ◽

10.24036/eksakta/vol21-iss2/230 ◽

2020 ◽

Vol 21 (2) ◽

pp. 124-130

Author(s):

Mhd Zalil Efendi ◽

Ramadhan Sumarmin ◽

M. Syukri Fadil

Keyword(s):

Wound Healing ◽

Adult Male ◽

Mus Musculus ◽

Male Mice ◽

Pharmacological Activities ◽

Active Compounds ◽

Randomized Design ◽

Wide Range ◽

Completely Randomized Design ◽

Etlingera Elatior

Torch ginger has a wide range of good antimicrobial, antioxidant, anticancer, larvicidal and repellent activities. Active compounds in Torch ginger that affect pharmacological activities are phenols, polyphenols, flavonoids, and terpenoids. Based on these ingredients Torch ginger can be used to heal wounds. This study aims to observe the effect of Torch ginger Umbut extract on wound healing in mice. This study hopes to add information about the effect of Torch ginger umbut extract on the healing of cuts in mice so that it can be another alternative for wound healing and can be a reference for other researchers. This study used a completely randomized design with 5 treatments and 3 replications. Tests carried out on adult male mice. The results showed that the optimal wound healing in P2 is treatment with 10% Torch ginger umbut extract which requires a range of wound healing 7-8 days. Based on these results, Torch ginger umbut extract can heal wounds.

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