RENDEMEN DAN pH GELATIN KULIT IKAN NILA (Oreochromis niloticus) YANG DIRENDAM PADA BERBAGAI KOSENTRASI HCl

Gelatin is derivative protein of collagen fiber which is found in the skin, bone, and cartilage. The composition of amino acids is almost similar to collagen, where glycine as the main amino acid and as 2/3 of all the amino acids that make it up, where 1/3 of the amino acids contained by proline and proline hydroxine. This research aims at knowing the physical charateristic of rendement and pH by using the skin of tilapia (Oreochromis niloticus) in concentration of HCL as immersion solution. The benefits of this research are expected to be the information sources of gelatin making in production scale, especially the gelatin production from the skin of tilapia (Oreochromis niloticus). The information are expected to support the development of information science and technology (IPTEK). Gelatin of tilapia skin (Oreochromis niloticus) used Completely Randomized Design (RAL) with 4 treatments. The treatments were HCl immersion to Tilapia’s skin (Oreochromis nilaticus) with N1 (4%), N2 (5%), N3 (6%) and N4 (7%) in 4 times repetitions

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PENGARUH PERBEDAAN SPESIES IKAN TERHADAP HIDROLISAT PROTEIN IKAN DENGAN PENAMBAHAN ENZIM PAPAIN (The Effect of Various Fish Species On Fish Protein Hydrolysate With The Addition of Papain Enzyme)

SAINTEK PERIKANAN Indonesian Journal of Fisheries Science and Technology ◽

10.14710/ijfst.13.1.24-30 ◽

2017 ◽

Vol 13 (1) ◽

pp. 24 ◽

Cited By ~ 1

Author(s):

Strata Annisa ◽

Yudhomenggolo Sastro Darmanto ◽

Ulfah Amalia

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Fish Species ◽

Research Method ◽

Protein Hydrolysate ◽

Amino Acid Profile ◽

Fish Protein ◽

Randomized Design ◽

Fish Protein Hydrolysate ◽

Completely Randomized Design

Hidrolisat protein ikan (HPI) dihasilkan dari proses penguraian protein ikan menjadi peptida sederhana maupun asam amino melalui proses hidrolisis oleh enzim, asam, atau basa. Enzim yang digunakan adalah enzim protease, baik yang berasal dari tanaman, hewan maupun mikroba. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan enzim papain terhadap karakteristik HPI dan menentukan optimum terbaik hidrolisat protein dari jenis ikan yang berbeda dengan penambahan enzim papain. Metode penelitian ini adalah experimental laboratories dengan menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang diterapkan adalah perbedaan jenis ikan yang meliputi ikan nila, ikan bandeng, dan ikan cucut. Data yang diperoleh dianalisis dengan menggunakan sidik ragam ANOVA dengan uji lanjut untuk menentukan nilai yang berpengaruh maupun tidak dengan uji BNJ (Beda Nyata Jujur). Hasil penelitian dari ketiga perlakuan didapatkan HPI terbaik yaitu rendemen HPI nila 5,64% dengan kadar protein 30,17%, kadar air 9,06%, dan viskositas 1,91 cP sedangkan profil asam amino terbanyak adalah asam glutamat terutama pada HPI ikan cucut sebesar 4,183%. Fish protein hydrolysate (FPH) is produced from the decomposition of fish protein become peptides and amino acids through hydrolysis by enzymes, acids or bases. The enzyme used are protease enzymes, both derived from plants, animals, and microbes. The purpose of this study was to determine the effect of papain enzyme addition on FPH characteristics and to determine the characteristics of the FPH and determine the best optimum of FPH from different fish species with the addition of the papain enzyme. This research method is experimental laboratories by using a Completely Randomized Design (CRD). The applied treatments applied are different fish species including tilapia, milkfish, and shark. The data obtained were analyzed by using ANOVA with further test to determine the value that influencd or not with BNJ test. The results of the research from the three treatments are yield FPH tilapia 5,64% with protein content 30,17%, water content 9,06%, and viscosity 1,91 cP, while the highest amino acid profile is glutamic acid, especially in FPH shark 4,183%.

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Energy values and standardized ileal digestibility of amino acids in some feedstuffs for broilers

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n6p2651 ◽

2019 ◽

Vol 40 (6) ◽

pp. 2651

Author(s):

Felipe Santos Dalólio ◽

Diego Ladeira Silva ◽

Luiz Fernando Teixeira Albino ◽

Ricardo Vianna Nunes ◽

Valdir Ribeiro Junior ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Control Diet ◽

Brown Rice ◽

Metabolizable Energy ◽

Experimental Unit ◽

Ileal Digestibility ◽

Randomized Design ◽

Completely Randomized Design ◽

Amino Acid Contents

The objective of this study was to determine the energy values, standardized ileal digestibility coefficients and digestible amino acid contents of parboiled brown rice bran (PBRB), protein sorghum meal (PSM) and bread waste meal (BWM) in broiler diets. In the first trial, 144 broiler chicks at 14 days of age were evaluated in a completely randomized design involving three feedstuffs + a control diet, with six replicates and six birds per experimental unit. The total excreta collection method was adopted to determine the energy values of the feedstuffs. In the second trial, 144 broilers at 24 days of age were used in a completely randomized design involving three feedstuffs + a protein-free diet, with six replicates and six birds per experimental unit. Ileal digesta was harvested to determine the standardized ileal digestibility coefficients and digestible amino acid contents of the feedstuffs. The nitrogen-corrected apparent metabolizable energy (AMEn) values of PBRB, PSM and BWM were 2638, 2799 and 3399 kcal.kg-1, respectively. The metabolizability coefficients of AMEn (CAMEn) of PBRB, PSM and BWM were 54.13, 57.35 and 86.66%, respectively. Bread waste meal had the highest CAMEn (p < 0.05) and standardized ileal digestibility of amino acids (p < 0.01), followed by PBRB and PSM. The main limiting digestible amino acids in PBRB, PSM and BWM were found at the following respective levels: methionine - 0.19, 0.33 and 0.24%; lysine - 0.40, 0.46 and 0.40%; threonine - 0.25, 0.50 and 0.45%; and valine - 0.44, 0.73 and 0.48%.

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Performance, gut morphometry and enzymatic activity of broilers fed neonatal supplementation in housing

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v40i1.38423 ◽

2018 ◽

Vol 40 (1) ◽

pp. 38423

Author(s):

Tatiane Souza dos Santos ◽

Adriano Barbieri ◽

Robert Guaracy Aparecido Cardoso Araujo ◽

Gustavo Do Valle Polycarpo ◽

Daniela Felipe Pinheiro ◽

...

Keyword(s):

Amino Acids ◽

Polynomial Regression ◽

Pancreatic Enzyme ◽

Broiler Chicks ◽

Quadratic Effect ◽

Linear Effect ◽

Intestinal Length ◽

Randomized Design ◽

Relative Organ Weight ◽

Completely Randomized Design

The objective was to evaluate the performance, relative organ weight, morphometry, intestinal length and pancreatic enzyme activity of broilers receiving neonatal supplementation. A total of 900 Cobb 500 broiler chicks was housed in 30 boxes of 2.5 m². The treatments were divided in a completely randomized design with six replications, and consisted of five inclusion levels (0.0, 2.5, 5.0, 7.5 and 10 grams per bird). The supplement used was based of amino acids, vitamins and minerals, which was provided on the ration on the first day in the housing. Statistical analysis was performed using SAS (2008) and when significant broken down using a polynomial regression. Due to the inclusion of the neonatal supplement in the period from one to seven and one to 21 days of age, the feed intake presented a linear effect and the weight gain, a quadratic effect. The relative weights of the gizzard and liver showed a quadratic effect, and the inclusions of 4.18 and 3.41 grams/bird provided the lowest weights, respectively. The jejunum villi height decreased with supplementation as well as the activity of pancreatic lipase. It can be concluded that neonatal supplementation benefited the performance of birds up to 21 days of age.

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Methionine plus cystine requirements for the maintenance and efficiency of utilization with tambaqui of different body weights

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044014920 ◽

2020 ◽

Vol 44 ◽

Author(s):

Neliane Galvão Porto ◽

Felipe Barbosa Ribeiro ◽

Jefferson Costa de Siqueira ◽

Marcos Antonio Delmondes Bomfim ◽

Rafael Silva Marchão ◽

...

Keyword(s):

Amino Acid ◽

Control Treatment ◽

Native Fish ◽

Amino Acid Requirement ◽

Treatment Groups ◽

Protein Retention ◽

Lack Of Information ◽

Randomized Design ◽

Body Weights ◽

Completely Randomized Design

ABSTRACT Methionine is an essential amino acid, and generally, the first limiting one in the diets of tambaqui, the main native fish produced in continental aquaculture in South America. However, there is a lack of information on their amino acid requirement, especially for maintenance and efficiency of utilization. The present study aimed to determine the requirement of [methionine plus cystine] for the maintenance and efficiency of utilization using tambaqui of different body weights. Two experiments were carried out using 250 tambaqui with weights of 100 and 300 g. They were distributed in a completely randomized design with four treatment groups (0.264, 0.396, 0.528, and 0.660% of digestible [Met + Cys]), five replications. The control treatment (CT) was performed with the addition of methionine at the first level (0.264%) until the second level was reached (0.396%). The maintenance requirement was determined by the regression analysis between the consumption of [Met + Cys] and protein retention equal to zero. The efficiency was determined using the value of the slope coefficient of the line obtained by the linear regression. The protein and methionine retention values of tambaqui weighing 100 to 300 g were compared by the parallelism test, and no difference was observed between the parameters of the equations indicating the need for only one equation to describe the responses to body weights. The requirement of digestible [Met + Cys] for the maintenance of tambaqui with weights from 100 to 300 g was 60.47 mg kg-0.7 day-1 and the efficiency of utilization of 42%.

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The Effect Of Rajalele Probiotic On Survival Rate Of Fish Juvenile Pangasius pangasius, Oreochromis niloticus, Cyprinus carpio

JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS ◽

10.36987/jpbn.v6i1.1597 ◽

2020 ◽

Vol 6 (1) ◽

pp. 88-95

Author(s):

Marlina Doloksaribu ◽

Robert Martua Simanjuntak ◽

Ied Hidayani Parinduri

Keyword(s):

Social Sciences ◽

Experimental Study ◽

Data Analysis ◽

Survival Rate ◽

Cyprinus Carpio ◽

Oreochromis Niloticus ◽

Fish Farming ◽

Randomized Design ◽

The Social ◽

Completely Randomized Design

The use of probiotics is certainly one of the solutions in overcoming the problem of increasing seed in fish farming. One of them is the use of probiotic king catfish, in this study tested on catfish, tilapia and goldfish. This research is an experimental study. The design used in this study was a Completely Randomized Design with 4 treatments 3 replications. Data analysis used Statistical Package for the Social Sciences version 15. The results of the analysis of the highest seed survival rate were obtained on Pangasius pangasius (95%), on Oreochromis niloticus (83,33 %), and Cyprinus carpio (80%). The treatment of Rajalele probiotics has a very significant effect (very significant) Fanalysis (23.01) > Ftable 0.01 (7.59) on the graduation of Pangasius pangasius, Oreochromis niloticus, and Cyprinus carpio. The conclusion of this study shows that the survival rate the highest in Pangasius pangasius

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PENGARUH PEMBERIAN PROBIOTIK Bacillus spp. MELALUI PAKAN TERHADAP KELANGSUNGAN HIDUP DAN LAJU PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v16i1.212 ◽

2016 ◽

Vol 16 (1) ◽

Author(s):

Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Oreochromis Niloticus ◽

Specific Growth ◽

Good Combination ◽

Randomized Design ◽

Bacillus Spp ◽

Completely Randomized Design ◽

Total Bacteria ◽

Different Doses

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase, total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.

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Pengaruh Suplementasi Probiotik A3-51 terhadap Derajat Imunitas Oreochromis niloticus Didasarkan pada Angka Kuman pada Ginjal Setelah Uji Tantang dengan Aeromonas hydrophila dan Aeromonas salmonicida achromogenes

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.126 ◽

2006 ◽

Vol 8 (2) ◽

pp. 144

Author(s):

Agus Irianto ◽

Hernayanti Hernayanti ◽

Ning Iriyanti

Keyword(s):

Aeromonas Hydrophila ◽

Oreochromis Niloticus ◽

Challenge Test ◽

Aeromonas Salmonicida ◽

Control Fish ◽

System Response ◽

Randomized Design ◽

Immune System Response ◽

Completely Randomized Design ◽

Number Of Bacteria

Objectives of this research was to know the effect of probiotic A3-51 supplementation on food to the total number of bacteria in kidney and mortality of Oreochromis niloticus after challenge test with Aeromonas hydrophila and Aeromonas salmonicida achromogenes. This research was consisted of two experiment, the first was to know the highest immunity level of fish based on the number of macrophage and its phagocytic activity. Meanwhile, the second was to know the immunity level of fish challenged with A. hydrophila and A. salmonicida achromogenes by intra-peritoneal injection. The experiment used Completely Randomized Design in triplicates. The result from the first experiment showed that the highest non spesific immune system response, by means number of macrophage, was found 21 days treatment. The second experiment showed that the highest total number of bacteria in kidney and mortality level were 20.23 x 108 cells/g and 46.67%, respectively, both was found in control fish injected intra-peritoneally with A. salmonicida achromogenes.

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Stocking densities and feeding strategies in shrimp and tilapia polyculture in tanks

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000800039 ◽

2013 ◽

Vol 48 (8) ◽

pp. 1088-1095 ◽

Cited By ~ 7

Author(s):

Bruno Rodrigo Simão ◽

Luis Otavio Brito ◽

Alex Sandro Campos Maia ◽

Laizy Cabral Miranda ◽

Celicina Maria da Silveira Borges Azevedo

Keyword(s):

Oreochromis Niloticus ◽

Feeding Strategies ◽

Feed Conversion ◽

Shrimp Culture ◽

Main Crop ◽

Randomized Design ◽

Marine Shrimp ◽

Completely Randomized Design ◽

Feed Conversion Rate ◽

Fish Performance

The objective of this work was to evaluate the performance of Pacific marine shrimp (Litopenaeus vannamei) and tilapia (Oreochromis niloticus), in a polyculture in tanks subjected to different stocking densities and feeding strategies, in comparison with monoculture. Two experiments were performed, at the same time, in a completely randomized design with three treatments and four replicates each. Treatments for experiment I were: monoculture with 10 shrimp per m² (10S:0T); polyculture with 10 shrimp and 0.5 tilapia per m² (10S:0.5T); and polyculture with 10 shrimp and 1 tilapia per m² (10S:1T). Shrimp was the main crop, and feed was provided based on shrimp biomass. Treatments for experiment II were: monoculture with 2 tilapia per m² (2T:0S); polyculture with 2 tilapia and 2.5 shrimp per m² (2T:2.5S); and polyculture with 2 tilapia and 5 shrimp per m² (2T:5S). Tilapia was the main crop, and feed was provided based on fish requirements. In the experiment I, tilapia introduction to shrimp culture resulted in lower shrimp growth and poor feed conversion rate. In experiment II, shrimp introduction to tilapia culture did not interfere with fish performance. Polyculture is more efficient with the combination of 2 tilapia and 2.5 or 5 shrimp per m² and feed based on fish requirements.

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PENINGKATAN DAYA TERIMA DAN KADAR PROTEIN NUGGET SUBSTITUSI IKAN LELE (CLARIAS BATRACHUS) DAN KACANG MERAH (VIGNA ANGULARIS)

Media Gizi Indonesia ◽

10.20473/mgi.v11i1.106-112 ◽

2017 ◽

Vol 11 (1) ◽

pp. 106

Author(s):

S R Widya Areta Humaniora Justisia ◽

Annis Catur Adi

Keyword(s):

Statistical Analysis ◽

Amino Acid ◽

Protein Content ◽

Essential Amino Acid ◽

Clarias Batrachus ◽

Vigna Angularis ◽

Treatment Combination ◽

Randomized Design ◽

Composition Formula ◽

Completely Randomized Design

Catfi sh and red beans are high in protein and essential amino acid than can be processed into nuggets and used as high-protein snack alternative. This research aimed to determine the effect of catfi sh and red beans toward the acceptance and protein content, as well as determine the best composition to produce a good quality nuggets. Type of research in the development formula using true experimental with a completely randomized design. There were four (4) types of treatment (combination of catfi sh, catfi sh bones, and red beans) with three (3) times replication. Panelist used was 35 untrained panelists. Product acceptance was tested using statistical analysis Kruskal Wallis and Mann Whitney (ƒ¿ . 0.05). The result of organoleptic tests showed the most preferred nugget is F1 (combination of catfi sh and red beans). Highest increased protein per 100g nugget is F1 (combination of catfi sh and red beans) with 14.5 gcompare to F0 (catfi sh) with 11,5 g, there was signifi cant difference between F0 and F1, F2, F3, F4 to the characteristic of odour (p = 0.000), texture (p = 0.008), fl avor (p = 0.000), and form (p = 0.000). The best composition formula is F1 (combination of catfi sh and red beans).The best formula in terms of its composition, acceptability, and nutritionalvalue was F1 (combination of catfi sh and red beans).Keywords: acceptance, catfi sh, red beans, protein

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Profile of Amino Acid and Fatty Acid from Different Type of Worm as Feed of Broodstock Candidates for Domesticated Uceng (Nemacheilus fasciatus)

Research Journal of Life Science ◽

10.21776/ub.rjls.2020.007.02.5 ◽

2020 ◽

Vol 7 (2) ◽

pp. 108-114

Author(s):

Lik Anatus Sholikah ◽

Agoes Soeprijanto ◽

Yuni Kilawati

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Fatty Acid ◽

Amino Acid ◽

Amino Acid Content ◽

Gonadal Development ◽

Lumbricus Rubellus ◽

Market Demand ◽

The People ◽

Randomized Design

Uceng fish (Nemacheilus fasciatus) is a fish that lives wild on the river. All this time, the people have only relied on fishing from nature to consume, but the high market demand for fish will increase river fishing activities in excess. One of the steps to suppress the decline in fish populations is domestication because currently, the fish is not yet cultivated. The domestication process by feeding worms containing amino acids and fatty acids accelerates broodstock candidates' gonadal development. This research aims to determine the type of worms suitable as feed for the growth and gonadal development of broodstock candidates. The method that used in this research is Completely Randomized Design (CRD) with 3 treatments and 3 repetitions. Based on the analysis of amino acids and fatty acids in each type of worm, the best results were obtained on Lumbricus rubellus with an amino acid content of 50.91 gr/100gr and fatty acid 292.27%.

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