scholarly journals Rituals of Containment: Many Pandemics, Body Politics, and Social Dramas During COVID-19 in Pakistan

2021 ◽  
Vol 6 ◽  
Author(s):  
Inayat Ali
Keyword(s):  
Scientific Evidence ◽  
The Body ◽  
Body Politics ◽  
Ethnographic Research ◽  
Purposive Sampling ◽  
Previous Argument ◽  
Interview Guide ◽  
Science And Politics ◽  
The Government ◽  
Visible Body

Infecting millions of people, causing around two million deaths, and affecting billions of people worldwide during January 2021, the coronavirus 2019 (COVID-19) pandemic is not merely one pandemic but many. These many pandemics, which I identify herein, have revealed the overt and subtle entanglements among religion, science, and politics around COVID-19. Building on my current ethnographic research on COVID-19 using purposive sampling and interview guide in Pakistan, and borrowing from various anthropological concepts such as “social drama,” proposed by Victor Turner, and ritual, I have developed a concept that I call rituals of containment. With this concept, I extend my previous argument regarding “symbolic ownership” to show a visible “body politics” by demonstrating how religion, science, and politics around COVID-19 are entangled at individual and government levels. This has become observable through the rituals of the Pakistani government of containment to deal with COVID-19. Such entanglements are visible in the case of strategies to tackle infected “viral bodies,” as the government has enacted its authority: (1) to bury what I am terming the dead viral body without its beloved ones present; (2) to return or not to return this body to family members in a coffin; (3) or to provide the grieving family with a symbolic empty coffin. These Covidian politics have led to the question: Who in actuality owns the body? In conclusion, I argue that the problem lies in the discriminatory and contradictory rituals of containment of the government, not in using scientific evidence and guidelines.

The Biopolitics of Sacrifice: Securing Infrastructure at the G20 Summit

2013 ◽  
Vol 30 (5) ◽  
pp. 45-69
Author(s):  
Alessandra Renzi ◽  
Greg Elmer
Keyword(s):  
Economic Growth ◽  
War On Terror ◽  
Global Financial Crisis ◽  
The Body ◽  
Body Politics ◽  
Growth Property ◽  
Control Techniques ◽  
Security Practices ◽  
The Government ◽  
Clash Of Cultures

This article investigates infrastructure spending from a biopolitical perspective and rethinks its connections to emerging regimes of (in)securitization. Starting with a study of the organization and contestation of the G8/G20 summits in Toronto in June 2010, the analysis moves through the shifty territory of a governmental logic that is reconfiguring the body politics of civic participation, as well as the ways in which discourses on economic growth, property and public safety intertwine in the aftermath of the 2008 global financial crisis. The case of Toronto shows significant changes to recent security practices, control techniques and funding arrangements. Rather than ‘war on terror’, ‘austerity and crisis’ are the new keywords sustaining current governmental rationality and the criminalization of dissent, which are no longer funded by defence budgets but by economic stimuli packages. This new rationality, while still relying on fear as an affective mode to mobilize masses, has exchanged a set of discourses on the clash of cultures or civilization for one on sacrifice. Following Foucault’s work on the government of populations and security, it is now possible to talk about ‘a sacrifice series’ to describe the series of elements that connect military or economic logics of (in)securitization.

scholarly journals Lytninger i Alternativ Behandling: En fænomenologisk analyse

2014 ◽  
Author(s):  
Inge Kryger Pedersen
Keyword(s):  
Alternative Medicine ◽  
Scientific Evidence ◽  
Healthcare Research ◽  
The Body ◽  
Physiological Data ◽  
Ethnographic Research ◽  
The Gaze ◽  
Based Medicine ◽  
Clinical Gaze ◽  
Analytical Practice

I den evidensbaserede medicins æra prioriteres tekniske færdigheder, standardise- ringer og behandlinger med videnskabeligt beviste virkninger i effektiviseringen af pleje og behandling. Ikke desto mindre er alternativ behandling, som hverken er reguleret, kontrolleret, standardiseret eller autoriseret og med yderst be- grænset videnskabelig evidens, meget populær i Danmark og andre lande. I denne artikel, der bygger på etnografiske undersøgelser inden for tre af de mest populære former for alternativ behandling i Danmark, undersøges lytning som fænomen med henblik på at bidrage til forklaringerne og forståelsen af behand- lingernes popularitet. Forskellige former for lytning som fænomenologiske og intersubjektive processer er i fokus: behandlernes lytning til deres klienter, klienternes lytning til deres behandlere og egen krop, forskerens lytning som en fænomenologisk analytisk praksis og undersøgelsesstrategi. Lytning er et overset område sammenlignet med blikket, for eksempel det medicinske eller kliniske blik i forskning inden for sundhedsområdet. I den eksisterende litteratur om lytning er der kun få undersøgelser af patienters eller brugeres oplevelser af lytning. Denne artikel anvender et lyttebegreb, der adskiller sig fra det at høre som en fysiologisk proces, for at analysere, hvad den lyttende gør, når lytning opleves – her af klienter i alternative behandlingsklinikker. Hvad er det for en praksis, der overbeviser klienten om at være forstået af behandleren? Alle sanser synes at være i spil, og lytning er mere end konversation. Brugere af alternativ behandling værdsætter behandleres inddragelse af andet end det, der bliver sagt, for eksempel opbygningen af relationen mellem behandler og klient og ikke kun vægtningen af fysiologiske data. Artiklen anlægger et dobbeltperspektiv ved at tage udgangspunkt i såvel brugeres erfaringer som forskerens egne erfaringer og fremgangsmåde. Således demonstreres værdien af at praktisere lytning i en behandlings-, men også i en fænomenologisk undersøgelsessammenhæng. Søgeord: alternativ behandling, brugerperspektiv, praksis, kropsfænomenologi, Merleau-Ponty.In the era of evidence based medicine, technical skills, standardization and healthcare tested by research are at a premium to help determine the effectiveness of caring and healing practice. However, alternative medicine, which is less rule-bound than official bio-medicine and based on very limited scientific evidence, represents an increasingly popular medical option for many Danes. In this article, which is based on in-depth ethnographic research on three of the most popular forms of session-based alternative medicine in Denmark the phenomenon of listening is highlighted to make this popularity intelligible. Different forms of listening as a phenomenological and intersubjective process are explored: practitioners’ listening to their clients, clients’ listening to their practitioners as well as to their own body and finally the researcher’s listening as a phenomenological analytical practice and strategy. Listening has been a much neglected area compared to the gaze (for example the medical or clinical gaze) in healthcare research. In the scarce literature on listening within healthcare, only few studies have explored patients’ perception of listening. Drawing on a concept of listening that distinguishes listening from hearing as a physiological process this article argues in favor of another element of listening, namely the practitioner’s involvement that gives the client a sensation of being understood (or listened to). The true listener uses all the senses and listening is more than conversation. Users of alternative treatments benefit when practitioners in their listening draw on the context, i.e. the practitioner-client relationship, and not only on physiological data. Addressing the users’ as well as the researcher’s perspective, this article demonstrates the value of paying more attention to different forms of listening, both in healthcare and in ethnographic research. Keywords: alternative medicine, user’s perspective, bodily practice, phenome- nology of the body, Merleau-Ponty.

scholarly journals Queensland making a splash: Memorial pools and the body politics of reconstruction

2016 ◽  
Vol 23 (2) ◽  
pp. 178-195
Author(s):  
Janina Gosseye ◽  
Alice Hampson
Keyword(s):  
Cultural Identity ◽  
Large Scale ◽  
Social Space ◽  
The Body ◽  
Body Politics ◽  
Way Of Life ◽  
Social Foundations ◽  
The Public ◽  
The Government ◽  
Post War

AbstractIn April 2015, The Pool emerged as the winning proposal for Australia's exhibition at the 2016 Venice Architecture Biennale.1 Creative directors Aileen Sage and Michelle Tabet explained that the pool was ‘a lens through which to explore Australian cultural identity’ and ‘aptly represents a distinctively Australian democratic and social space’.2 In Australia, the public pool was popularised in the post-war period, particularly in Queensland where it offered relief from the long, hot and humid summers. Although Brisbane already had several floating baths along the Brisbane River from the mid-nineteenth century, large-scale, in-ground pool construction in the state did not start in earnest until the mid-1950s, when the personal and social benefits of recreational time with family and friends became well established. In Queensland, as elsewhere in the country, the government encouraged the construction of swimming pools, and many became memorial pools, dedicated to those who had fought to defend an Australian ‘way of life’. Their design was to reflect the civic and social foundations of the initiative, and in Queensland architects took delight in all the opportunities it afforded. The result was a widely diverging collection of predominantly humble and economical structures that were rarely ordinary or dull. Analysing three key pools that were constructed in regional Queensland between 1955 and 1965 — in Rockhampton, Mackay and Miles — this article draws out some of the defining features of Queensland's modern memorial pools, and highlights how this typology became the quintessential ‘Australian democratic and social space’.3

Efektivitas Aroma Terapi Lemon untuk Menangani Emesis Gravidarum

2019 ◽  
Vol 11 (4) ◽  
pp. 277-284
Author(s):  
Vitrianingsih Vitrianingsih ◽  
Sitti Khadijah
Keyword(s):  
Pregnant Women ◽  
Sampling Technique ◽  
T Test ◽  
The Body ◽  
Nausea And Vomiting ◽  
P Value ◽  
Purposive Sampling ◽  
Body Tissues ◽  
Quasi Experimental ◽  
Paired T Test

Studi memperkirakan emesis gravidarum terjadi pada 50-90% kehamilan. Mual muntah pada kehamilan memberikan dampak yang signifikan bagi tubuh dimana ibu menjadi lemah, pucat dan cairan tubuh berkurang sehingga darah menjadi kental (hemokonsentrasi). Keadaan ini dapat memperlambat peredaran darah dan berakibat pada kurangnya suplay oksigen serta makanan ke jaringan sehingga dapat membahayakan kesehatan ibu dan janin. Salah satu terapi yang aman dan dapat dilakukan untuk mengurangi keluahan mual muntah pada ibu hamil adalah pemberian aromaterapi lemon. Penelitian bertujuan untuk mengetahui efektifitas aroma terapi lemon untuk menangani emesis gravidarum. Penelitian ini menggunakan rancangan Quasi experiment  dengan  one group pre-post test design. Populasi penelitian adalah ibu hamil yang mengalami emesis gravidarum di Kecamatan Berbah, Sleman. Jumlah sampel 20 ibu hamil trimester pertama yang diambil dengan teknik purposive sampling. Pengukuran mual muntah dilakukan debelum dan setelah  pemberian aromaterapi lemon menggunakan Indeks Rhodes. Analisa data menggunakan uji Paired t-test. Hasil penelitian didapatkan rata-rata skor mual muntah sebelum pemberian aromaterapi lemon berdasarkan Indeks Rhodes pada Ibu Hamil dengan emesis gravidarum yaitu 22,1 dan terjadi penurunan skor setelah pemberian aromaterapi lemon menjadi 19,8. Ada pengaruh pemberian aromaterapi lemon dengan pengurangan mual muntah pada ibu hamil (p-value = 0.017). Berdasarkan hasil penelitian dapat disimpulkan pemberian aromaterapi lemon efektif untuk mengurangi emesis gravidarum pada ibu hamil trimester pertama.  Kata kunci: aromaterapi lemon, emesis gravidarum THE EFFECTIVENESS OF LEMON AROMATHERAPY FOR HANDLING EMESIS GRAVIDARUM   ABSTRACT Studies estimate that nausea and vomiting (emesis gravidarum) occur in 50 – 90% of pregnancies. Nausea and vomiting of pregnancy have a significant impact on the body in which it makes a mother becomes weak, pale, and decreasing body fluid so that the blood becomes thick (hemoconcentration). This situation can slow down blood circulation and inflict the lack of oxygen and food supplies to the body tissues so that it can endanger the health of the mother and fetus. One of the therapies that is safe and can be conducted to reduce nausea and vomiting of pregnancy is by giving the lemon aromatherapy treatment. The research aims to determine the effectiveness of the aroma of lemon therapy to deal with emesis gravidarum. This study applied quasi-experimental research with one group pretest-posttest design. The population of this study was pregnant women who experienced emesis gravidarum. Furthermore, samples were 20 mothers from Berbah, Sleman taken by using a purposive sampling technique. Nausea and vomiting were assessed between before and after giving lemon aromatherapy using the Rhodes Index. The data were analyzed using the paired t-test. The mean score of nausea and vomiting before giving lemon aromatherapy on mother with emesis gravidarum based on the Rhodes Index was 22.1. However, it decreased after given lemon aromatherapy treatment to 19.8. Therefore, there was an effect on giving lemon aromatherapy treatment toward the decrease of nausea and vomiting for pregnant women (p-value = 0.017). Lemon aromatherapy is effective to reduce emesis gravidarum.  Keywords: lemon aromatherapy, emesis gravidarum

ANALISIS YURIDIS NORMATIF PUTUSAN PERKARA MAHKAMAH AGUNG KONSTITUSI RI No. 004/PUU-II/2004

2013 ◽  
Vol 7 (1) ◽  
pp. 7
Author(s):  
Doni Budiono
Keyword(s):  
The Body ◽  
Supreme Courts ◽  
Military Justice ◽  
State Administration ◽  
The Supreme Court ◽  
Directorate General ◽  
The Government ◽  
Government Rules ◽  
The Right ◽  
Tax Court

The  authority  of justice in Indonesia  is executed by  the Supreme Courts and  the  justice  boards/body under the Supreme Courts, including  the general  justice, religious affairs justice, military justice,  state administration  justice,  and  the Constitution Court. According to  certainty in  the Act of  Tax Court, Article1, clause  (5),  tax  dispute   refers to the legal dispute arising in the  taxation  affairs between the  tax payer or the  body  responsible for the  tax with   the government   executives  ( Directorate General of Tax) as the consequence of   the issue of  the decree for the  appeal  to the Tax  Court in accordance with the  tax Act, including the  charge  against the  execution of collection   in accordance with the  Act of Tax Collection by force. The  formation of Tax Court is  designed by  the Executives, in this case, the  Department of Finance, specifically  the Directorate   General  of Tax  which has the right to issue  law  more technical about  tax accord to Article 14,  letter A,  President Decree  no. 44  year 1974,  concerning the  basic  organization of the Department.  Based on  it,  it  is clear that  in addition to execute the government  rules and policy,  this body  has to execute judicial   rules and policy. This is against the  principles of  Judicative  Power/Authority in Indonesia,  which   clearly states that this body  should be under the Supreme Court.   Therefore. It is suggested that   the Act  No UU no.14 Year 2012 concerning  Tax Court   be revised  in accordance with the system of  Power Division  of Justice  as  stated in 45 Constitutions.

Implementasi Kebijakan Agraria dan Ketimpangan Penguasaan Lahan (Kasus Lahan Eks HGU di Desa Cipeuteuy, Kecamatan Kabandungan, Kabupaten Sukabumi, Provinsi Jawa Barat)

2020 ◽  
Vol 4 (6) ◽  
pp. 731
Author(s):  
Muhammad Febri Ramdani
Keyword(s):  
Policy Implementation ◽  
Land Tenure ◽  
Quantitative Method ◽  
Qualitative Data ◽  
Agrarian Reform ◽  
Purposive Sampling ◽  
Reform Agenda ◽  
Agrarian Policy ◽  
Strong Relation ◽  
The Government

ABSTRAKMUHAMAD FEBRI RAMDANI. Implementasi Kebijakan Agraria dan Ketimpangan Penguasaan Lahan (Kasus Lahan Eks HGU di Desa Cipeuteuy, Kecamatan Kabandungan, Kabupaten Sukabumi, Provinsi Jawa Barat). Dibimbing oleh MARTUA SIHALOHO.Redistribusi lahan yang dilakukan oleh pemerintah sebuah upaya implementasi kebijakan agraria. Kebijakan agraria tersebut berupa asset reform (penataan aset) eks lahan perkebunan dengan skema legalisasi aset berwujud sertifikasi bidang lahan. Namun dalam pelaksanannya pemerintah mengklaim bahwa kebijakan tersebut merupakan agenda reforma agraria. Atas dasar klaim tersebut, penting untuk meninjau access reform (penataan akses) bekerja, karena pada hakikatnya reforma agraria merupakan asset reform (penataan aset) dan access reform (penataan akses) yang berjalan beriringan, dengan bertujuan untuk menata ketimpangan penguasaan lahan agar terwujudnya keadilan agraria (agrarian justice). Penelitian ini dilakukan dengan metode kuantitatif yang didukung kualitatif dengan pendekatan sensus. Metode yang digunakan untuk menentukan responden ialah purposive sampling dengan teknik non probability sampling. Pengolahan data menggunakan uji statistik rank spearman untuk melihat hubungan variabel. Hasil penelitian yang diperoleh menunjukan bahwa tingkat asset reform berada pada kategori rendah. Hasil uji statistik menunjukan bahwa asset reform berhubungan dengan access reform dan asset reform berhubungan dengan ketimpangan penguasaan lahan.Kata kunci: access reform, asset reform, keadilan agraria, reforma agrariaABSTRACTMUHAMAD FEBRI RAMDANI. Implementation of Agrarian Policy and Land Tenure Inequality (Case of Ex-HGU Land in Cipeuteuy Village, Kabandungan District, Sukabumi Regency, West Java Province). Supervised by MARTUA SIHALOHO.Land redistribution has been done by government as an effort of agrarian policy implementation. The policy came in form of asset reform of ex-plantation land with asset legalization scheme (land-part certification). But the government claimed that this policy is one of the agrarian reform agenda. It is important to observe how this access reform works, because agrarian and access reform can’t be separated one another, with purpose to reduce the inequality of land tenure so that the agrarian justice can be reach. This research used quantitative method supported by qualitative data, using the census approachment. Purposive sampling with non probabilty sampling used to specify the respondent. The data processed by rank spearman statistic test to analyze the relation between variable. This research shows a low level of asset reform. The statistic test shows that the low asset reform have a strong relation to low access reform, and high inequality of land tenure.Key words: access reform, agrarian justice, agrarian reform, asset reform

Perbedaan Efektivitas Pemberian Origami Dan Playdough Terhadap Perkembangan Pada Anak Prasekolah Kelompok A Di TK Aisyiyah Bustanul Athfal Kota Kediri

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Halimatus Saidah ◽  
Yunida Septiyanty
Keyword(s):  
Child Development ◽  
Sampling Technique ◽  
The Body ◽  
Purposive Sampling ◽  
Organ Systems ◽  
Post Test ◽  
Group A ◽  
Key Words Development ◽  
Children Development ◽  
Research Show

ABSTRAKPerkembangan (development) adalah bertambahnya kemampuan atau fungsi semua sistem organ tubuh sebagai akibat bertambahnya kematangan atau maturitas fungsi sistem organ tubuh (Dewi, 2013). Tujuan penelitian ini adalah mengetahui perbedaan efektivitas pemberian origami dan playdough terhadap perkembangan pada anak prasekolah kelompok A di TK Aisyiyah Bustanul Athfal tahun 2018.Desain penelitian yang digunakan adalah penelitian pre eksperiment dengan pendekatan pre-test and post-test Design. Populasi yang diteliti adalah seluruh anak kelompok A di TK Aisyiyah Bustanul Athfal berjumlah 56 anak dengan teknik purposive sampling diperoleh sampel 36 responden. Instrumen penelitian yang digunakan adalah lembar KPSP. Hasil penelitian kemudian dianalisa dengan menggunakan wilcoxon signed rank.Hasil penelitian yang dilakukan menunjukan bahwa perkembangan anak sebelum pelaksanaan pemberian Origami didapatkan setengahnya perkembangan anak meragukan, setelah pelaksanaan didapatkan hampir seluruhnya perkembangan anak sesuai. Perkembangan anak sebelum pelaksanaan pemberian Playdough didapatkan sebagian besar perkembangan anak meragukan, setelah pelaksanaan didapatkan sebagian besar perkembangan anak sesuai. Hasil analisis menunjukkan ada pengaruh pemberian permainan origami dan permainan Playdough terhadap perkembangan anak kelompok A di TK Aisyiyah Bustanul Athfal Tahun 2018 dengan hasil ρ-value = 0,001 ɑ = 0,05 dari kelompok origami dan ρ-value = 0,007 ɑ = 0,05 dari kelompok playdough, sedangkan hasil analisis perbedaan adanya perbedaan efektivitas pengaruh pemberian permainan origami dan playdough terhadap perkembangan anak pada kelompok A di TK Aisyiyah Bustanul Athfal Tahun 2018 dengan hasil ρ-value = 0,043 ɑ = 0,05.Berdasarkan hasil penelitian dapat disimpulkan ada pengaruh permainan origami dan playdough terhadap perkembangan anak pada kelompok A di TK Aisyiyah Bustanul Athfal Tahun 2018. Diharapkan kepada orang tua maupun guru untuk lebih meningkatkan frekuensi dalam memberi stimulus permainan terutama permainan origami yang diberikan kepada anak. Kata Kunci : Perkembangan , Anak Prasekolah,  Origami, Playdough ABSTRACTDevelopment is increasing ability or function of all organ systems of the body as a result of increasing maturity or maturity function of the organ system of the body (Dewi, 2013). The purpose of this research is to know the effectiveness difference of origami and playdough on development in preschoolers group A in Aisyiyah Bustanul Athfal Kindergarten in 2018.The research design used is research pre eksperiment with approach pre-test dan post-test. The population studied was all group A children in kindergarten Aisyiyah Bustanul Athfal amounted to 56 children with purposive sampling technique obtained sample 36 respondents. The research instrument used is KPSP sheet. The results were then analyzed by using wilcoxon signed rank.The results of the research show that the development of children before the implementation of giving Origami found half of child development doubt, after the implementation is obtained almost entirely the child's development accordingly. Child development prior to the implementation of Playdough gift obtained most of the development of children doubt, after the implementation is obtained most of the child's development accordingly.  The results of the analysis show that there is an effect of giving origami game and Playdough game to the child development group A in Aisyiyah Bustanul Athfal Kindergarten Year 2018 with the result ρ-value = 0.001 ɑ = 0.05 from the origami group and ρ-value = 0.007 ɑ = 0.05 of the playdough group, while the result of difference analysis that is difference between origami and playdough influence to children development in group A diiyah Aisyiyah Bustanul Athfal Year 2018 with result of ρ-value = 0,043 ɑ = 0,05.Based on the research results can be concluded there is influence of origami and playdough on the development of children in group A in Aisyiyah Bustanul Athfal Kindergarten Year 2018. Expected to parents and teachers to increase the frequency of giving stimulus especially the origami given to the child. Key Words : Development, children preschool, Origami, Playdough

Personhood and youth-making in contemporary Indigenous Amazonia

2020 ◽  
Author(s):  
Pirjo Kristiina Virtanen ◽  
Alessandra Severino da Silva Manchinery
Keyword(s):  
Young People ◽  
Global South ◽  
The Body ◽  
Body Politics ◽  
Indigenous Youth ◽  
Social Responsibilities ◽  
Brazilian Amazonia ◽  
University Studies ◽  
Social Belonging ◽  
Indigenous Voices

This essay looks at the construction of personhood in Brazilian Amazonia from the perspective of Indigenous youth. In Amazonian sociocosmology, personhood is constructed relationally, a process in which the body is a distinctive factor. Consequently, during schooling and university studies, young people have responded to and resisted representations and policies that have often silenced Indigenous voices and limited their fabrication of bodies. The contemporary social responsibilities of Indigenous youth and the challenges faced in undertaking them shape how their subjectivity, agency, and recognized social belonging are being constantly increased, removed, or even denied. The essay draws from anthropological theories of relational personhood, as well as ideas of geo- and body-politics present in theorizing on the Global South.

scholarly journals Subcellular Localization and Vesicular Structures of Anthocyanin Pigmentation by Fluorescence Imaging of Black Rice (Oryza sativa L.) Stigma Protoplast

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 685
Author(s):  
Enerand Mackon ◽  
Yafei Ma ◽  
Guibeline Charlie Jeazet Dongho Epse Mackon ◽  
Qiufeng Li ◽  
Qiong Zhou ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Subcellular Localization ◽  
Oryza Sativa L ◽  
Scientific Evidence ◽  
Brown Rice ◽  
The Body ◽  
Central Vacuole ◽  
In Planta ◽  
Black Rice

Anthocyanins belong to the group of flavonoid compounds broadly distributed in plant species responsible for attractive colors. In black rice (Oryza sativa L.), they are present in the stems, leaves, stigmas, and caryopsis. However, there is still no scientific evidence supporting the existence of compartmentalization and trafficking of anthocyanin inside the cells. In the current study, we took advantage of autofluorescence with anthocyanin’s unique excitation/emission properties to elucidate the subcellular localization of anthocyanin and report on the in planta characterization of anthocyanin prevacuolar vesicles (APV) and anthocyanic vacuolar inclusion (AVI) structure. Protoplasts were isolated from the stigma of black and brown rice and imaging using a confocal microscope. Our result showed the fluorescence displaying magenta color in purple stigma and no fluorescence in white stigma when excitation was provided by a helium–neon 552 nm and emission long pass 610–670 nm laser. The fluorescence was distributed throughout the cell, mainly in the central vacuole. Fluorescent images revealed two pools of anthocyanin inside the cells. The diffuse pools were largely found inside the vacuole lumen, while the body structures could be observed mostly inside the cytoplasm (APV) and slightly inside the vacuole (AVI) with different shapes, sizes, and color intensity. Based on their sizes, AVI could be grouped into small (Ф < 0.5 um), middle (Ф between 0.5 and 1 um), and large size (Ф > 1 um). Together, these results provided evidence about the sequestration and trafficking of anthocyanin from the cytoplasm to the central vacuole and the existence of different transport mechanisms of anthocyanin. Our results suggest that stigma cells are an excellent system for in vivo studying of anthocyanin in rice and provide a good foundation for understanding anthocyanin metabolism in plants, sequestration, and trafficking in black rice.

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