scholarly journals Comparative Phytochemical Analyses and Metabolic Profiling of Different Phenotypes of Chinese Cabbage (Brassica Rapa ssp. Pekinensis)

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 587 ◽  
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Soo-Yun Park ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
High Performance ◽  
Total Carotenoids ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Phenolic Contents ◽  
Flight Mass Spectrometry ◽  
The Relationship

This study aimed to comprehensively examine the interface between primary and secondary metabolites in oval- and rectangular-shaped Chinese cabbage (Brassica rapa ssp. pekinensis) using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In addition to differences in shape, there was significant morphological variation between the two cultivars. The rectangular variety had greater height and deeper green color, whereas the oval variety had more leaves and greater width. A total of 42 primary metabolites identified by GC-TOFMS were subjected to partial least-squares discriminant, which indicated significant differences in the primary and secondary metabolisms of the two cultivars. Furthermore, total glucosinolate and phenolic contents were higher in the oval cultivar, whereas the rectangular cultivar contained a higher level of total carotenoids. This metabolome study comprehensively describes the relationship between primary and secondary metabolites in the oval and rectangular cultivars of Chinese cabbage and provides information useful for developing strategies to enhance the biosynthesis of glucosinolates, phenolics, and carotenoids in Chinese cabbage. Additionally, this work highlights that HPLC and GC-TOFMS–based metabolite profiling is suitable techniques to determine metabolic differences in Chinese cabbage.

scholarly journals Metabolomic analysis reveals the interaction of primary and secondary metabolism in white, pale green, and green pak choi (Brassica rapa subsp. chinensis)

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Hyeon Ji Yeo ◽  
Seung-A Baek ◽  
Ramaraj Sathasivam ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
High Performance ◽  
Reducing Power ◽  
Total Phenolic ◽  
Pak Choi ◽  
Primary And Secondary Metabolites ◽  
Chlorophyll Contents ◽  
Significant Difference ◽  
Pale Green

AbstractThis study aimed to comprehensively analyze primary and secondary metabolites of three different-colored (white, pale green, and green) pak choi cultivars (Brassica rapa subsp. chinensis) using gas chromatography attached with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In total, 53 primary metabolites were identified and subjected to partial least-squares discriminant analysis. The result revealed a significant difference in the primary and secondary metabolites between the three pak choi cultivars. In addition, 49 hydrophilic metabolites were detected in different cultivars. Total phenolic and glucosinolate contents were highest in the pale green and green cultivars, respectively, whereas total carotenoid and chlorophyll contents were highest in the white cultivar. Superoxide dismutase activity, 2,2-diphenyl-1-picrylhydraz scavenging, and reducing power were slightly increased in the white, pale green, and green cultivars, respectively. In addition, a negative correlation between pigments and phenylpropanoids was discovered by metabolite correlation analysis. This approach will provide useful information for the development of strategies to enhance the biosynthesis of phenolics, glucosinolates, carotenoids, and chlorophyll, and to improve antioxidant activity in pak choi cultivars. In addition, this study supports the use of HPLC and GC-TOFMS-based metabolite profiling to explore differences in pak choi cultivars.

scholarly journals Comparative GC-MS analysis of the composition of metabolites of aboveground and underground parts of Comarum palustre L

2021 ◽  
Vol 10 (4) ◽  
pp. 95-103
Author(s):  
J. Strugar ◽  
A. A. Orlova ◽  
M. N. Povydysh
Keyword(s):  
Secondary Metabolites ◽  
Raw Materials ◽  
Biologically Active ◽  
Quantitative Composition ◽  
Leningrad Region ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Plant Raw Materials ◽  
Comarum Palustre ◽  
The Relationship

Introduction. Understanding the mechanisms of accumulation of individual groups of biologically active substances in promising types of plant raw materials and the possibility of predicting them is important for solving fundamental and applied problems of pharmaceuticals. To date, differences have been revealed in the qualitative and quantitative composition of secondary metabolites in the aboveground and underground of Comarum palustre L., however, the issue remains unstudied.Aim. Comparative metabolomic study of the composition of the primary metabolites of the aboveground and underground parts of Comarum palustre L.Materials and methods. The object of the study was the aboveground and underground parts of Comarum palustre L., harvested in the vicinity of the nursery of medicinal plants of the St. Petersburg State Chemical and Pharmaceutical University (Leningrad Region, Vsevolozhsky District, Priozerskoe Highway, 38 km) in 2019 and dried. Metabolomic studies based on GC-MS method was perfomed. A statistical analysis based on the MetaboAnalyst 5.0 platform was used.Results and discussion. Analysis of the chromatograms obtained using the GC-MS method revealed the content of 933 primary metabolites in the aboveground and underground parts of Comarum palustre L., 120 of which were identified. Using a number of statistical methods, 10 metabolites from monosaccharides, acids and alcohols, making the greatest contribution to the manifestation of differences between the studied samples, were identified.Conclusion. The study revealed the relationship between the composition of primary and secondary metabolites in medicinal plant raw materials.

scholarly journals INFLUENCE OF TEMPERATURE CONDITIONS ON THE ACCUMULATION OF PRIMARY AND SECONDARY METABOLITES BY LICHENS FLAVOCETRARIA CUCULLATA AND CETRARIA LAEVIGATA

2021 ◽  
pp. 227-233
Author(s):  
Il'ya Andreyevich Prokopiev ◽  
Igor Vitalievich Sleptsov ◽  
Lena Nikolayevna Poryadina
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Unsaturated Fatty Acids ◽  
Effect Of Temperature ◽  
Gas Chromatography Mass Spectrometry ◽  
Climatic Chamber ◽  
Protective Properties ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
General Activation

The study of the effect of temperature on the accumulation of primary and secondary metabolites by lichens Flavocetraria cucullata (Bellardi) Kärnefelt & Thell and Cetraria laevigata Rass was carried out. Lichen samples were taken out from under the snow (-20 °C) together with the soil substrate and transferred to the climatic chamber. Then the temperature in the climatic chamber was sequentially increased to +10 and +20 °C. The lichen exposure was carried out for 30 days for each temperature regime. The analysis of primary metabolites was performed by gas chromatography-mass spectrometry. It was shown that in lichens F. cucullata and C. laevigata at temperatures of +10 and +20 °C, an increase in the content of mannitol, ribitol, sucrose, and hydroxyproline was observed, as well as a decrease in the content of unsaturated fatty acids as compared to the initial samples. At the same time, the content of glycerol and arabitol in the thalli of the initial lichens (-20 °C) was higher than after exposure at +10 and +20 °C, which, apparently, is associated with the cryoprotective properties of these compounds. The content of secondary metabolites in lichens was determined by high performance liquid chromatography. It was shown that the content of usnic, allo-protolichesterinic, and protolichesterinic acids in F. cucullata increased after exposure in a climatic chamber, which may be associated with a general activation of metabolic processes with an increase in temperature. At the same time, the content of fumarprotocetraric acid in C. laevigata lichens decreased at temperatures of +10 and +20 °C compared to the initial samples, which may be associated with its protective properties under the action of low-temperature stress.

scholarly journals Impact of Raspberry (Rubus idaeus L.) Primocane Tipping on Fruit Yield and Quality

2017 ◽  
Vol 45 (2) ◽  
pp. 417-424 ◽  
Author(s):  
Zala ZORENC ◽  
Robert VEBERIC ◽  
Darinka KORON ◽  
Maja MIKULIC-PETKOVSEK
Keyword(s):  
Secondary Metabolites ◽  
Fruit Quality ◽  
High Performance ◽  
Great Influence ◽  
High Temperature Stress ◽  
Quality Parameters ◽  
Rubus Idaeus ◽  
Fruit Yield ◽  
Primary And Secondary Metabolites ◽  
The Impact

High temperature stress, which has been occurring more often in recent years, usually coincides with the flowering of primocane raspberries and causes a negative effect on fruit quality parameters. One of the methods of delaying raspberry flowering and fruit development to avoid high summer temperatures is tipping the young primocanes. The aim of the study was to investigate how this practice affects the fruit characteristics and primary and secondary metabolites of two primocane raspberry cultivars (‘Amira’ and ‘Polka’). For this purpose, we performed primocane tipping on two different dates in late spring and analyzed the berries from three subsequent sampling dates. High performance liquid chromatography-mass spectrometry (HPLC-MS) analyses were used for the identification and quantification of individual phenolic compounds and HPLC analyses for individual sugars and organic acids. Primocane tipping had great influence on the beginning of the harvest season of both cultivars. The impact on fruit yield was insignificant. Sampling date had a greater influence on fruit metabolite contents than did different treatments, with cultivar ‘Polka’ showing a greater response to primocane tipping than cultivar 'Amira'. Based on primary and secondary metabolites, it is difficult to say which treatment provided the best results, since dissimilar patterns were shown at different sampling dates and between cultivars. With negligible differences in fruit quality, primocane tipping was shown to be a good cultivation practice for delaying the production season of raspberries.

scholarly journals Comparative Metabolic Profiling of Grape Pulp during the Growth Process Reveals Systematic Influences under Root Restriction

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 377
Author(s):  
Feng Leng ◽  
Shuyan Duan ◽  
Shiren Song ◽  
Liping Zhao ◽  
Wenping Xu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
High Performance ◽  
Developmental Stages ◽  
Grape Berry ◽  
Vitis Vinifera L ◽  
Pulp Tissue ◽  
Primary And Secondary Metabolites ◽  
Amino Acids And Derivatives ◽  
Root Restriction

The compositions and contents of metabolites in the pulp tissue play critical roles in the fruit quality for table grape. In this study, the effects of root restriction (RR) on the primary and secondary metabolites of pulp tissue at five developmental stages were studied at the metabolomics level, using “Red Alexandria” grape berry (Vitis vinifera L.) as materials. The main results were as follows: 283 metabolites were annotated by using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS); 28 and 16 primary metabolites contents were increased and decreased, and 11 and 19 secondary metabolites contents were increased and decreased, respectively, along the berry development; RR significantly decreased 12 metabolites (four amino acids and derivatives, three organic acids, four flavonoids and one other compound) contents, and improved 40 metabolites (22 amino acids and derivatives, six nucleotides, four carbohydrates, four cofactors, three cinnamic acids and one other compound) accumulation at the different developmental stages. Altogether, our study would be helpful to increase our understanding of grape berry’s responses to RR stress.

scholarly journals The Effect of Different Drying Methods on Primary and Secondary Metabolites in Korean Mint Flower

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 698
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Chanung Park ◽  
Yong Suk Chung ◽  
Sang Un Park
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
High Performance ◽  
Freeze Drying ◽  
Sinapic Acid ◽  
Drying Methods ◽  
Oven Drying ◽  
Primary And Secondary Metabolites ◽  
Freeze Dried ◽  
Β Carotene

Edible flowers have been used in the food and beverage industries because of their high nutritional value, flavor, and scent. For the storage of edible flowers used in these industries, drying is a necessity to store the materials more easily and prevent the damage of metabolites in the flowers. However, drying may affect metabolite retention because drying conditions can differ according to the various methods. In this study, Agastache rugosa flowers were dried using four different methods (oven drying at 25 ± 1 °C, 50 ± 1 °C, 80 ± 1 °C, and freeze drying) and primary and secondary metabolites were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOF/MS). Freeze-dried flower samples contained higher levels of carotenoids (lutein, 13Z-β-carotene, β-carotene, and 9Z-β-carotene) and phenolics (rosmarinic acid, ferulic acid, and sinapic acid). Contrarily, the 80 °C oven-dried flower samples contained higher levels of most amino acids and flavonoids (including acacetin and tilianin) and at 25 °C and 50 °C contained higher levels of carbohydrates. Therefore, freeze-drying is a suitable method for retaining carotenoids and phenolics. In contrast, oven drying at 50 °C was highly recommended to retain amino acids and flavonoids.

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